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12 results on '"Letko, Juraj"'

7. Mitostatin is down-regulated in human prostate cancer and suppresses the invasive phenotype of prostate cancer cells.

Author

Fassan, Matteo, D'Arca, Domenico, Letko, Juraj, Vecchione, Andrea, Gardiman, Marina P, McCue, Peter, Wildemore, Bernadette, Rugge, Massimo, Shupp-Byrne, Dolores, Gomella, Leonard G, Morrione, Andrea, Iozzo, Renato V, Baffa, Raffaele, Fassan, Matteo, D'Arca, Domenico, Letko, Juraj, Vecchione, Andrea, Gardiman, Marina P, McCue, Peter, Wildemore, Bernadette, Rugge, Massimo, Shupp-Byrne, Dolores, Gomella, Leonard G, Morrione, Andrea, Iozzo, Renato V, and Baffa, Raffaele

Abstract

MITOSTATIN, a novel putative tumor suppressor gene induced by decorin overexpression, is expressed in most normal human tissues but is markedly down-regulated in advanced stages of mammary and bladder carcinomas. Mitostatin negatively affects cell growth, induces cell death and regulates the expression and activation levels of Hsp27. In this study, we demonstrated that ectopic expression of Mitostatin in PC3, DU145, and LNCaP prostate cancer cells not only induced a significant reduction in cell growth, but also inhibited migration and invasion. Moreover, Mitostatin inhibited colony formation in soft-agar of PC3 and LNCaP cells as well as tumorigenicity of LNCaP cells in nude mice. Conversely, targeting endogenous Mitostatin by siRNA and anti-sense strategies in PC3 and DU145 prostate cancer cells enhanced the malignant phenotype in both cell lines. In agreement of these anti-oncogenic roles, we discovered that Mitostatin was absent in ∼35% (n = 124) of prostate tumor samples and its overall reduction was associated with advanced cancer stages. Collectively, our findings indicate that MITOSTATIN may acts as a tumor suppressor gene in prostate cancer and provide a novel cellular and molecular mechanism to be further exploited and deciphered in our understanding of prostate cancer progression.

Published
2011

8. Mitostatin Is Down-Regulated in Human Prostate Cancer and Suppresses the Invasive Phenotype of Prostate Cancer Cells

Author

Fassan, Matteo, primary, D'Arca, Domenico, additional, Letko, Juraj, additional, Vecchione, Andrea, additional, Gardiman, Marina P., additional, McCue, Peter, additional, Wildemore, Bernadette, additional, Rugge, Massimo, additional, Shupp-Byrne, Dolores, additional, Gomella, Leonard G., additional, Morrione, Andrea, additional, Iozzo, Renato V., additional, and Baffa, Raffaele, additional

Published
2011
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12. Effects of Oral, Vaginal, and Transdermal Hormonal Contraception on Serum Levels of Coenzyme Q10, Vitamin E, and Total Antioxidant Activity.

Author

Palan, Prabhudas R., Strube, Felix, Letko, Juraj, Sadikovic, Azra, and Mikhail, Magdy S.

Subjects
VAGINAL contraceptives, SIDE effects of oral contraceptives, UBIQUINONES, VITAMIN E, ANTIOXIDANTS, ETHINYL estradiol, FREE radicals, OXIDATIVE stress, NUCLEIC acids, T-test (Statistics), ANALYSIS of variance
Abstract

The use of the transdermal contraceptive patch is associated with greater bioavailability of ethinyl estradiol (EE) compared with contraceptive vaginal ring or oral contraceptives (OC). We compared the influences of three contraceptive methods (OC, vaginal ring, and transdermal patch) on serum levels of coenzyme Q10, α-tocopherol, γ-tocopherol and total antioxidant capacity in premenopausal women. Blood samples from 30 premenopausal women who used hormonal contraception for at least 4 months were collected. Forty subjects who did not use any contraception were studied as control. Serum levels of coenzyme Q10, α- tocopherol and γ-tocopherol were measured by high-pressure liquid chromatography. Serum samples were also assayed for total antioxidant capacity (TAOC). Serum levels of coenzyme Q10 and α-tocopherol were found to be significantly lower (P < .05) in all three contraceptive users compared with controls. Contraceptive patch users had the lowest levels of coenzyme Q10 levels compared with normal subjects. Serum TAOC levels were significantly lower (P < .05) among the contraceptive user groups. Alterations in coenzyme Q10 and α-tocopherol induced by hormonal contraception and the potential effect(s) of exogenous ovarian hormones should be taken into consideration in future antioxidant research. [ABSTRACT FROM AUTHOR]

Published
2010
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