1. BRCA mutations cause reduction in miR-200c expression in triple negative breast cancer
- Author
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Ismet Tasdelen, Gulsah Cecener, Gulcin Tezcan, Unal Egeli, Berrin Tunca, Elif Erturk, Sahsine Tolunay, Sehsuvar Gokgoz, Uludağ Üniversitesi/Sağlık Meslek Yükseokulu/Tıbbi Laboratuvar Teknikleri Programı., Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Patoloji Anabilim Dalı., Ertürk, Elif, Çeçener, Gülşah, Tezcan, Gülçin, Egeli, Ünal, Tunca, Berrin, Gökgöz, Şehsuvar, Tolunay, Şahsine, Taşdelen, İsmet, AAK-3371-2021, AAP-9988-2020, AAH-3843-2020, AAH-1420-2021, ABI-6078-2020, EXK-4525-2022, AAI-1612-2021, and EBN-1186-2022
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Vascular Endothelial Growth Factor A ,Mırn200 microrna, human ,Resistance ,Triple Negative Breast Neoplasms ,Bioinformatics ,Subclass ,Metastasis ,Cancer growth ,Pathology ,Mirnlet7 microRNA, human ,Triple negative breast cancer ,Disease course ,skin and connective tissue diseases ,Down-regulation ,Triple-negative breast cancer ,Zeb1 ,Gene expression regulation ,Genetics & Heredity ,Progression ,BRCA1 Protein ,Circulating Microrna ,Stomach Neoplasms ,Breast Neoplasms ,MicroRNA ,General Medicine ,Middle Aged ,Epithelial-mesenchymal transition ,MicroRNA-200c ,Gene expression profiling ,Gene Expression Regulation, Neoplastic ,Vascular endothelial growth factor A ,Reverse transcription polymerase chain reaction ,Disease Progression ,Female ,BRCA1/2 gene mutations ,Feedback loop ,Human ,Adult ,Clinical article ,Tumor invasion ,Vasculotropin A ,Ovarian-cancer ,MiR-200c ,Biology ,Article ,Breast cancer ,VEGFA protein, human ,microRNA ,Genetics ,medicine ,Humans ,Family ,Gene mutation ,Human tissue ,Oncogene ,Loss function ,BRCA1 protein, human ,BRCA2 Protein ,Neoplastic ,BRCA mutation ,medicine.disease ,BRCA2 protein, human ,Let 7a gene ,MicroRNAs ,VEGFA gene ,Mutation ,Cancer research ,Controlled study - Abstract
Triple negative breast cancer (TNBC) is the most aggressive and poorly understood subclass of breast cancer (BC). Over the recent years, miRNA expression studies have been providing certain detailed overview that aberrant expression of miRNAs is associated with TNBC. Although TNBC tumors are strongly connected with loss of function of BRCA genes, there is no knowledge about the effect of BRCA mutation status on miRNA expressions in TNBC cases. The aims of this study were to evaluate the expression profile of miRNAs that plays role in TNBC progression and the role of BRCA mutations in their regulation. The expression level of BC associated 13 miRNAs was analyzed in 7 BRCA mutations positive, 6 BRCA mutations negative TNBC cases and 20 non-tumoral tissues using RT-PCR. According to RT2 Profiler PCR Array Data Analysis, let-7a expression was 4.67 fold reduced in TNBCs as compared to normal tissues (P = 0.031). In addition, miR-200c expression was 5.75 fold reduced in BRCA mutation positive TNBC tumors (P = 0.005). Analysis revealed a negative correlation between miR-200c and VEGFA expressions (r = -468). Thus, miR-200c may be involved in invasion and metastasis in TNBC cases with BRCA mutation. In this study we provide the knowledge on the first report of association between microRNA-200c and BRCA mutations in TNBC. Further studies and evaluations are required, but this miRNA may provide novel therapeutic molecular targets for TNBC treatment and new directions for the development of anticancer drugs.
- Published
- 2015
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