Search

Global sustainability rests on a myriad of benefits provided by natural ecosystems that support human livelihoods and well-being, from biodiversity persistence to climate regulation. The undeniable importance of conserving tropical forests has drawn most of the conservation spotlight towards it. However, open ecosystems such as the Brazilian Campo Rupestre (rupestrian grassland), have been historically overlooked despite their high diversity and key associated ecosystem services. We highlight major current threats to the persistence of the Campo Rupestre emphasizing its ecological, social, cultural, geo-environmental, and economic importance. We call attention to the importance of the Campo Rupestre as a reservoir of biodiversity and ecosystem services and offer priority actions that resulted from discussions involving scientists, industry representatives, environmental managers, and other members of civil society. Proposed actions include efforts related to ecological restoration, sustainable ecotourism, protection of traditional ecological knowledge, identification of emerging research questions, and development of tailored public policies. Such issues are integrated into a framework that collectively represents a road map to safeguard the Campo Rupestre from further degradation and steer its historical overexploitation towards sustainable management. Safeguarding the future of non-forest biomes like this poses a challenge to current paradigms of nature conservation. By establishing priorities and guidelines, we propose an actionable plan, which we hope can support informed decision-making policy towards a sustainable use of the Campo Rupestre.

Abstract The Cerrado (Brazilian Savanna) biome suffers intense degradation due to several anthropic activities and its recovery is extremely important for the restoration of environmental quality. Therefore, the present study tested the survival and growth of seedlings of the grass Schizachyrium tenerum produced by the division of clumps. The experiment was carried out in a greenhouse using Reddish Oxisol, dystrophic of “cerrado sensu stricto” and Litholic Neosol dystrophic of quartzitic rupestrian grassland with and without the addition of limestone and NPK. Survival of S. tenerum was also compared between being grown in a greenhouse and directly in the field in full sun. Seedlings of S. tenerum had greater survival, total growth, number of tillers and accumulation of biomass when cultivated in Reddish Oxisol and Reddish Oxisol with limestone added than in the other treatments. Seedling survival was three times greater when grown in a greenhouse than in full sun.

AbstractINTRODUCTION:Despite chemical and physical vector control strategies, persistent infestations of Triatoma sordida have been reported in a large part of Minas Gerais, Brazil, and the cause for this is little investigated. We aimed to characterize the deltamethrin toxicological profile in peridomestic T. sordidapopulations from Triângulo Mineiro area of Minas Gerais.METHODS:Susceptibility to deltamethrin was assessed in seventeen peridomestic T. sordida populations. Serial dilutions of deltamethrin in acetone (0.2µL) were topically applied on the first instar nymphs (F1; five days old; fasting weight, 1.2 ± 0.2mg). Dose response results were analyzed using Probit software, and the lethal doses, slope and resistance ratios were determined. Qualitative tests were also performed.RESULTS:The deltamethrin susceptibility profile of T. sordida populations revealed resistance ratios ranging from 0.84 to 2.8. The percentage mortality in response to a diagnostic dose was 100.0% in all populations.CONCLUSIONS:From our results, the lack of resistance to insecticides but persistent T. sordida infestations in the Triângulo Mineiro area may be because of: 1) environmental degradation facilitating dispersion of T. sordida , allowing colonization in artificial ecotopes; 2) operational failures; and 3) complexity of the peridomicile in the study area.These variables are being investigated.

INTRODUCTION: Triatoma brasiliensis is the species of greatest epidemiological relevance in the semi-arid region of Brazil. This species is predominantly found in domestic environments, and it has the ability to build large colonies with high levels of natural infection via Trypanosoma cruzi. Thus, T. brasiliensis is one of the most efficient transmitters of Chagas disease (CD) to humans. Despite household spraying with residual insecticides, many areas report persistent reinfestations for reasons that remain poorly understood. Therefore, this study sought to characterize the toxicological profile of deltamethrin in T. brasiliensis from areas with persistent reinfestation in State of Ceará, Brazil. METHODS: The susceptibility reference lineage (SRL) was derived from Umari. Serial dilutions of deltamethrin were prepared and applied to the dorsal abdomen of first instar nymphs. The control group received only pure acetone. Mortality was evaluated after 72h. Qualitative tests assessed mortality in response to a diagnostic dose of 1xLD99 (0.851 nanograms of active ingredient per treated nymph) of the SRL. RESULTS: The susceptibility profile characterization of the T. brasiliensis populations revealed 50% resistance ratios (RR50) that ranged from 0.32 to 1.21. The percentage of mortality in response to the diagnostic dose was 100%. CONCLUSIONS: We demonstrated that T. brasiliensis was highly susceptible to deltamethrin. The control difficulties found might be related to the recolonization of the triatomines originating from neighboring environments and the possible operational failures related to the process of spraying that enabled specimens less susceptible to deltamethrin to survive.

Introduction Triatoma sordida is the most captured Triatomine species in the Brazilian artificial environment. In 2008, the discovery of three Triatomine populations with altered susceptibilities to deltamethrin highlighted the importance of investigating the genetic potential for resistance in triatomines. The purpose of this study was to characterize the susceptibility to deltamethrin of peridomestic T. sordida populations in Minas Gerais, Brazil. Methods A susceptibility reference lineage derived from Uberaba, Minas Gerais, Brazil was used. Serial dilutions of deltamethrin were prepared and applied to the dorsal abdomen of first instar nymphs. The control group received only pure acetone. Mortality was evaluated after 72h. Qualitative tests assessed mortality in response to a diagnostic dose of 1xLD99 of the susceptibility reference lineage. Results Susceptibility profile characterization of T. sordida populations revealed resistance ratios (RR50s) ranging from 0.42 to 3.94. The percentage mortality in response to the diagnostic dose varied from 70% to 100%. A comparison of the results obtained in the quantitative and qualitative assays demonstrated a lack of correspondence for some populations. Conclusions We demonstrated that only T. sordida populations that present a RR50>1.0 have altered susceptibility, and the execution of simultaneous field and laboratory tests is required to understand the actual effect of vector control. A possible cause of the observed resistance ratios might be the continuous use of pyrethroids in Brazil since the 1980s.

Abstract Background Triatoma sordida, a vector of Trypanosoma cruzi, is native of Brazil, Bolivia, Paraguay, Argentina, and Uruguay, and occurs primarily in peridomiciles. Currently, it is the species most frequently captured by the Chagas Disease Control Program in Brazil. For this reason, population genetic studies attract great interest, as they can provide further information about the dispersal and household invasion processes of this species. In the absence of suitable markers, the objective of this study was to test the cross amplification of microsatellite primers. Findings 23 primers were tested for microsatellite loci already described for other species of the genus Triatoma sp. Forty four specimens of T. sordida captured in the north of Minas Gerais were used to validate the use of standardized loci for population genetic analyses. It was possible to amplify 10 of the 23 loci tested for T. sordida. Conclusions This is the first study that provides 10 microsatellite markers for population analysis of this triatomine species. Cross-amplification of primers can be used among other phylogenetically related species whose loci are already available for study.

After controlling Triatoma infestans in Brazil, other species of triatomine that were considered minor in the transmission of Chagas disease became important. The persistence of Triatoma brasiliensis in Northeastern Brazil, associated with reinfection of domestic environments recently sprayed with pyrethroids, may be a signal of susceptibility alteration of this species to this insecticide. Specimens of T. brasiliensis from the municipality of Tauá, state of Ceará, were captured before and one year after spraying. They were submitted to bioassays using deltamethrin. The LD50 ranged from 0.19-0.33 ng of deltamethrin/nymph. The resistance ratio among samples from Tauá varied from 1.16-1.79 in the samples captured before the spraying and 1.00-1.74 in the samples captured one year after spraying, demonstrating that the two populations were equally susceptible to deltamethrin. The small difference in susceptibility between the two captures suggests that T. brasiliensis obtained in the second capture are from new invasions of the domestic environment and that the insecticide did not select resistant individuals. Therefore, it is suggested that T. brasiliensis control be carried out supplementing the regular use of pyrethroids with complementary measures, such as improvement of the dwellings and health education.

Wastewater Treatment Plant (WWTP) generate pasty wastes, known biosolids, which can be toxic and recalcitrant, motivating studies aiming at their degradation. Filamentous fungi were investigated to degradation of biosolids from a WWTP in the Goiânia, Goiás, Brazil. All grew in the presence of biosolids, being inhibited by increase in concentration, except SXS629, which increased proportionally to the concentration. All grew in the middle with biosolids at the original pH (10.5), although the correction (6.8) provided higher growth. Except SXS90, the others (SXS37, SXS615 and SXS628) degraded the biosolid, growing in medium containing biosolids as the only source of carbon; highlighting SXS628, whose growth in the biosolids exceeded the control. All evaluated isolates synthesize at least two prospected enzymes, especially SXS630 and SXS634, which synthesize all (carboxymethyl cellulase, tannase, polyphenoloxidase). This shows the potential use of these isolates (combined or not) in biotechnological processes aiming at the degradation of biosolids, especially SXS37.