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13. Influence of Core ?-1,2-Xylosylation on Glycoprotein Recognition by Murine C-type Lectin Receptors and Its Impact on Dendritic Cell Targeting

Author

Brzezicka K., Vogel U., Serna S., Johannssen T., Lepenies B., and Reichardt N.-C.

Abstract

Targeting antigens to dendritic cell subsets is a promising strategy to enhance the efficacy of vaccines. C-type lectin receptors (CLRs) expressed by dendritic cells are particularly attractive candidates since CLR engagement may promote cell uptake and may further stimulate antigen presentation and subsequent T cell activation. While most previous approaches have involved antibody-mediated CLR-targeting, glycan-based CLR targeting has become more and more attractive in recent years. In the present study, we show that small structural glycan modifications may markedly influence CLR recognition, dendritic cell targeting, and subsequent T cell activation. A biantennary N-glycan (G0) and its analogous O-2 core xylosylated N-glycan (XG0) were synthesized, covalently conjugated to the model antigen ovalbumin, and analyzed for binding to a set of murine CLR-Fc fusion proteins using lectin microarray. To evaluate whether the differential binding of G0 and XG0 to CLRs impacted dendritic cell targeting, uptake studies using murine dendritic cells were performed. Finally, effects of the ovalbumin glycoconjugates on T cell activation were measured in a dendritic cell/T cell cocultivation assay. Our results highlight the utility of glycan-based dendritic cell targeting and demonstrate that small structural differences may have a major impact on dendritic cell targeting efficacy. ¸ 2016 American Chemical Society.

Published
2016

14. The C-Type Lectin Receptor SIGNR3 Binds to Fungi Present in Commensal Microbiota and Influences Immune Regulation in Experimental Colitis

Author

Eriksson, M., Johannssen, T., von Smolinski, D., Gruber, A., Seeberger, P., and Lepenies, B.

Subjects
C-type lectin receptor, colitis, Immunology, carbohydrate recognition, microbiota, SIGNR3, Immunology and Allergy, fungi, host innate immunity
Abstract

Inflammatory bowel disease is a condition of acute and chronic inflammation of the gut. An important factor contributing to pathogenesis is a dysregulated mucosal immunity against commensal bacteria and fungi. Host pattern- recognition receptors (PRRs) sense commensals in the gut and are involved in maintaining the balance between controlled responses to pathogens and overwhelming innate immune activation. C-type lectin receptors (CLRs) are PRRs recognizing glycan structures on pathogens and self-antigens. Here we examined the role of the murine CLR specific intracellular adhesion molecule-3 grabbing non-integrin homolog-related 3 (SIGNR3) in the recognition of commensals and its involvement in intestinal immunity. SIGNR3 is the closest murine homolog of the human dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) receptor recognizing similar carbohydrate ligands such as terminal fucose or high-mannose glycans. We discovered that SIGNR3 recognizes fungi present in the commensal microbiota. To analyze whether this interaction impacts the intestinal immunity against microbiota, the dextran sulfate sodium-induced colitis model was employed. SIGNR3(-/-) mice exhibited an increased weight loss associated with more severe colitis symptoms compared to wild-type control mice. The increased inflammation in SIGNR3(-/-) mice was accompanied by a higher level of TNF-α in colon. Our findings demonstrate for the first time that SIGNR3 recognizes intestinal fungi and has an immune regulatory role in colitis.

Published
2013
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15. Chemical synthesis of phosphatidylinositol mannoside glycans from Mycobacterium tuberculosis

Author

Seeberger, P., Boonyarattanakalin, S., and Lepenies, B.

Subjects
humanities
Abstract

The efficient synthesis of phosphatidylinositol mono- to hexa-mannoside (PIM1 to PIM6) is reported. The invention relates to these phosphatidylinositol mono- to hexa-mannosides carrying a linker and a reactive functional group, e.g. the sulfhydryl group, a protein, a fluorescent probe, or a solid phase. The invention further relates to vaccines comprising the PIMs linked to a carrier protein or an antigen.

Published
2010

17. The classical lancefield antigen of group a streptococcus is a virulence determinant with implications for vaccine design

Author

Sorge, N.M. van, Cole, J.N., Kuipers, K., Henningham, A., Aziz, R.K., Kasirer-Friede, A., Lin, L., Berends, E.T., Davies, M.R., Dougan, G., Zhang, F., Dahesh, S., Shaw, L., Gin, J., Cunningham, M., Merriman, J.A., Hütter, J., Lepenies, B., Rooijakkers, S.H., Malley, R., ., Walker, M.J., Shattil, S.J., Schlievert, P.M., Choudhury, B., Nizet, V., Sorge, N.M. van, Cole, J.N., Kuipers, K., Henningham, A., Aziz, R.K., Kasirer-Friede, A., Lin, L., Berends, E.T., Davies, M.R., Dougan, G., Zhang, F., Dahesh, S., Shaw, L., Gin, J., Cunningham, M., Merriman, J.A., Hütter, J., Lepenies, B., Rooijakkers, S.H., Malley, R., ., Walker, M.J., Shattil, S.J., Schlievert, P.M., Choudhury, B., and Nizet, V.

Abstract

Item does not contain fulltext

Published
2014

26. Advances in the Chemical Synthesis of Carbohydrates and Glycoconjugates

Author

Malik, Ankita, Seeberger, Peter H., Varón Silva, Daniel, Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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27. Enzymatic Synthesis of Glycans and Glycoconjugates

Author

Rexer, Thomas, Laaf, Dominic, Gottschalk, Johannes, Frohnmeyer, Hannes, Rapp, Erdmann, Elling, Lothar, Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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28. Interplay of Carbohydrate and Carrier in Antibacterial Glycoconjugate Vaccines

Author

Moeller, Tyler D., Weyant, Kevin B., DeLisa, Matthew P., Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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29. Glyco-Engineering of Plant-Based Expression Systems

Author

Fischer, Rainer, Holland, Tanja, Sack, Markus, Schillberg, Stefan, Stoger, Eva, Twyman, Richard M., Buyel, Johannes F., Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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30. Engineering of Yeast Glycoprotein Expression

Author

De Wachter, Charlot, Van Landuyt, Linde, Callewaert, Nico, Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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31. Bacterial Glycoengineering as a Biosynthetic Route to Customized Glycomolecules

Author

Yates, Laura E., Mills, Dominic C., DeLisa, Matthew P., Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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32. Recombinant Proteins and Monoclonal Antibodies

Author

Jefferis, Roy, Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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33. Glycobiotechnology of the Insect Cell-Baculovirus Expression System Technology

Author

Palomares, Laura A., Srivastava, Indresh K., Ramírez, Octavio T., Cox, Manon M. J., Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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34. Animal Cell Expression Systems

Author

Butler, M., Reichl, U., Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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35. Glycoengineering of Mammalian Expression Systems on a Cellular Level

Author

Heffner, Kelley M., Wang, Qiong, Hizal, Deniz Baycin, Can, Özge, Betenbaugh, Michael J., Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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36. Glycoproteomics Technologies in Glycobiotechnology

Author

Alagesan, Kathirvel, Hoffmann, Marcus, Rapp, Erdmann, Kolarich, Daniel, Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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37. State-of-the-Art Glycomics Technologies in Glycobiotechnology

Author

Pralow, Alexander, Cajic, Samanta, Alagesan, Kathirvel, Kolarich, Daniel, Rapp, Erdmann, Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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38. Impact of Protein Glycosylation on the Design of Viral Vaccines

Author

Schön, Kathleen, Lepenies, Bernd, Goyette-Desjardins, Guillaume, Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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39. Glycan Array Technology

Author

Martinez, Juana Elizabeth Reyes, Thomas, Baptiste, Flitsch, Sabine Lahja, Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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40. Correction to: Animal Cell Expression Systems

Author

Butler, M., Reichl, U., Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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41. Erratum to: Animal Cell Expression Systems

Author

Butler, M., Reichl, U., Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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42. Correction to: Enzymatic Synthesis of Glycans and Glycoconjugates

Author

Rexer, Thomas, Laaf, Dominic, Gottschalk, Johannes, Frohnmeyer, Hannes, Rapp, Erdmann, Elling, Lothar, Scheper, Thomas, Series Editor, Belkin, Shimshon, Editorial Board Member, Bley, Thomas, Editorial Board Member, Bohlmann, Jörg, Editorial Board Member, Gu, Man Bock, Editorial Board Member, Hu, Wei-Shou, Editorial Board Member, Mattiasson, Bo, Editorial Board Member, Olsson, Lisbeth, Editorial Board Member, Seitz, Harald, Editorial Board Member, Silva, Ana Catarina, Editorial Board Member, Ulber, Roland, Editorial Board Member, Zeng, An-Ping, Editorial Board Member, Zhong, Jian-Jiang, Editorial Board Member, Zhou, Weichang, Editorial Board Member, Rapp, Erdmann, editor, Reichl, Udo, editor, Alagesan, K., With Contri.By, Betenbaugh, M. J., With Contri.By, Butler, M., With Contri.By, Buyel, J. F., With Contri.By, Cajic, S., With Contri.By, Callewaert, N., With Contri.By, Can, Ö., With Contri.By, Cox, M. M. J., With Contri.By, DeLisa, M. P., With Contri.By, De Wachter, C., With Contri.By, Elling, L., With Contri.By, Fischer, R., With Contri.By, Flitsch, S. L., With Contri.By, Frohnmeyer, H., With Contri.By, Gottschalk, J., With Contri.By, Goyette-Desjardins, G., With Contri.By, Heffner, K. M., With Contri.By, Hizal, D. B., With Contri.By, Hoffmann, M., With Contri.By, Holland, T., With Contri.By, Jefferis, R., With Contri.By, Kolarich, D., With Contri.By, Laaf, D., With Contri.By, Lepenies, B., With Contri.By, Malik, A., With Contri.By, Martinez, J. E. R., With Contri.By, Mills, D. C., With Contri.By, Moeller, T. D., With Contri.By, Palomares, L. A., With Contri.By, Pralow, A., With Contri.By, RamÚrez, O. T., With Contri.By, Rapp, E., With Contri.By, Reichl, U., With Contri.By, Rexer, T., With Contri.By, Sack, M., With Contri.By, Schillberg, S., With Contri.By, Schön, K., With Contri.By, Seeberger, P. H., With Contri.By, Srivastava, I. K., With Contri.By, Stoger, E., With Contri.By, Thomas, B., With Contri.By, Twyman, R. M., With Contri.By, Van Landuyt, L., With Contri.By, Varón Silva, D., With Contri.By, Wang, Q., With Contri.By, Weyant, K. B., With Contri.By, and Yates, L. E., With Contri.By

Published
2021
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43. The C-type lectin receptor MINCLE interferes with eosinophil function and protective intestinal immunity in Strongyloides ratti-infected mice.

Author

Linnemann L, Antwi-Ekwuruke J, Gnanapragassam V, Bang C, Rühlemann M, Ruland J, Hartmann W, Heepmann L, Dörken S, Yunus SM, Viebrock B, Schlosser A, Lepenies B, and Breloer M

Abstract

Strongyloides ratti is a helminth parasite that displays tissue-migrating and intestinal life stages. Myeloid C-type lectin receptors (CLRs) are pattern recognition receptors that recognize pathogen-derived ligands and initiate immune responses. To date, the role of CLRs in S. ratti infection has not been investigated. Here, we show that S. ratti-derived ligands are recognized by the CLR Macrophage inducible Ca 2+ -dependent lectin receptor (MINCLE). While MINCLE-deficiency did not affect initiation of a protective anti-S. ratti type 2 immunity, MINCLE-deficient mice had a transient advantage in intestinal immunity. Unravelling the underlying mechanism, we show that next to macrophages, dendritic cells and neutrophils, a fraction of eosinophils express MINCLE and expand during S. ratti infection. MINCLE-deficient eosinophils exhibited a more active phenotype and prolonged expansion in vivo and displayed increased capacity to reduce S. ratti motility and produce reactive oxygen species in vitro, compared to wild-type (WT) eosinophils. Depletion of eosinophils in S. ratti-infected mice after the tissue-migration phase elevated intestinal worm burden in MINCLE-deficient mice to the WT level. Thus, our findings establish a central contribution of eosinophils to parasite ejection from the intestine and suggest that S. ratti-triggered signalling via MINCLE interferes with eosinophil mediated ejection of S. ratti from the intestine., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)

Published
2024
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44. Myeloid C-type lectin receptors in host-pathogen interactions and glycan-based targeting.

Author

Stegmann F and Lepenies B

Subjects
Humans, Animals, Immunity, Innate, Myeloid Cells metabolism, Lectins, C-Type metabolism, Polysaccharides metabolism, Host-Pathogen Interactions
Abstract

Lectin-glycan interactions play a crucial role in the immune system. An important class of lectins in the innate immune system is myeloid C-type lectin receptors (CLRs). Myeloid CLRs act as pattern recognition receptors and are predominantly expressed by myeloid cells, such as macrophages, dendritic cells, and neutrophils. In innate immunity, CLRs contribute to self/non-self discrimination. While the recognition of pathogen-associated molecular patterns (PAMPs) by CLRs may contribute to a protective immune response, CLR engagement can also be exploited by pathogens for immune evasion. Since various CLRs act as endocytic receptors and trigger distinct signaling pathways in myeloid cells, CLR targeting has proven useful for drug/antigen delivery into antigen-presenting cells and the modulation of immune responses. This review covers recent discoveries of pathogen/CLR interactions and novel approaches for CLR targeting within the period of the past two years., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Published
2024
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45. Dose-dependent inactivation of Plasmodium falciparum in red blood cell concentrates by treatment with short-wavelength ultraviolet light.

Author

Fischer S, Zilkenat S, Rosse M, Schulze TJ, Seltsam A, Handke W, Lepenies B, and Gravemann U

Subjects
Humans, Malaria, Falciparum parasitology, Dose-Response Relationship, Radiation, Animals, Ultraviolet Rays, Plasmodium falciparum radiation effects, Erythrocytes parasitology, Erythrocytes radiation effects
Abstract

Background and Objectives: Plasmodium species are naturally transmitted by Anopheles mosquitos. The parasite infects red blood cells (RBCs) and can be transfused with blood products. In non-endemic areas, the main risk of infection arises from travellers coming back and people immigrating from malaria-endemic regions. Endemic countries face a permanent risk of infection from transfusion-transmitted malaria (TTM). TTM may cause life-threatening complications in patients dependent on blood donations. This study aimed to investigate the efficacy of Plasmodium falciparum inactivation in RBC units by treatment with short-wavelength ultraviolet C (UVC) light in the absence of photochemical additives., Materials and Methods: RBC units were spiked with P. falciparum to a parasite density of 0.1%-1% and irradiated with up to 4.5 J/cm 2 UVC. The parasite density of UVC-treated dilution series and untreated controls were compared over 3 weeks after irradiation., Results: The lowest dose of 1.5 J/cm 2 UVC led to a 3.1 log reduction in parasite load compared with the untreated control. The inactivation capacity was dose-dependent. Strikingly, 4.5 J/cm 2 led to ≥5.3 log unit reduction, which was equivalent to a complete inactivation in two out of three experiments., Conclusion: Pathogen reduction with UVC light was previously shown to be effective for different bacteria and viruses, but the inactivation of parasites in RBC concentrates was not addressed until now. The present study provides evidence for significant inactivation of P. falciparum-infected RBCs by UVC light., (© 2024 The Author(s). Vox Sanguinis published by John Wiley & Sons Ltd on behalf of International Society of Blood Transfusion.)

Published
2024
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46. Legionella pneumophila modulates macrophage functions through epigenetic reprogramming via the C-type lectin receptor Mincle.

Author

Stegmann F, Diersing C, and Lepenies B

Abstract

Legionella pneumophila is a pathogen which can lead to a severe form of pneumonia in humans known as Legionnaires disease after replication in alveolar macrophages. Viable L. pneumophila actively secrete effector molecules to modulate the host's immune response. Here, we report that L. pneumophila -derived factors reprogram macrophages into a tolerogenic state, a process to which the C-type lectin receptor Mincle (CLEC4E) markedly contributes. The underlying epigenetic state is characterized by increases of the closing mark H3K9me3 and decreases of the opening mark H3K4me3, subsequently leading to the reduced secretion of the cytokines TNF, IL-6, IL-12, the production of reactive oxygen species, and cell-surface expression of MHC-II and CD80 upon re-stimulation. In summary, these findings provide important implications for our understanding of Legionellosis and the contribution of Mincle to reprogramming of macrophages by L. pneumophila ., Competing Interests: The authors declare no competing interest., (© 2024 The Author(s).)

Published
2024
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47. Metabolic modulation: Pneumocystis phosphoglucomutase is a target influencing host recognition.

Author

Kottom TJ, Carmona EM, Lepenies B, and Limper AH

Abstract

Herein, this manuscript explores the significance of the phosphoglucomutase (PGM) enzyme in Pneumocystis spp., focusing on its role in fungal surface mannoprotein formation. Through expression of the Pneumocystis murina Pmpgm2 in a Saccharomyces cerevisiae pgm2Δ strain, we demonstrate restoration of binding to the mannose receptor (MR) and macrophages to wildtype yeast levels in this complemented strain. Gas Chromatography-Mass Spectroscopy (GC-MS) confirmed reduced mannose content in the pgm2Δ yeast strain compared to the wild-type and complemented Pmpgm2 cDNA-expressing strains. This study underscores fungal PGM function in dolichol glucosyl phosphate biosynthesis, crucial for proper cell wall mannoprotein formation. Furthermore, highlighting the conservation of targetable cysteine residues across fungal pathogens, PGM inhibition maybe a potential therapeutic strategy against a broad spectrum of fungal infections., Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Andrew H Limper reports financial support was provided by National Institutes of Health. Theodore J Kottom reports financial support was provided by National Institutes of Health. Eva Carmona reports financial support was provided by National Institutes of Health. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Author(s).)

Published
2024
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48. Secreted NS1 proteins of tick-borne encephalitis virus and West Nile virus block dendritic cell activation and effector functions.

Author

Camarão AAR, Gern OL, Stegmann F, Mulenge F, Costa B, Saremi B, Jung K, Lepenies B, Kalinke U, and Steffen I

Abstract

The flavivirus non-structural protein 1 (NS1) is secreted from infected cells into the circulation and the serum levels correlate with disease severity. The effect of secreted NS1 (sNS1) on non-infected mammalian immune cells is largely unknown. Here, we expressed recombinant sNS1 proteins of tick-borne encephalitis virus (TBEV) and West Nile virus (WNV) and investigated their effects on dendritic cell (DC) effector functions. Murine bone marrow-derived DCs (BMDCs) showed reduced surface expression of co-stimulatory molecules and decreased release of pro-inflammatory cytokines when treated with sNS1 of TBEV or WNV prior to poly(I:C) stimulation. Transcriptional profiles of BMDCs that were sNS1-exposed prior to poly(I:C) stimulation showed two gene clusters that were downregulated by TBEV or WNV sNS1 and that were associated with innate and adaptive immune responses. Functionally, both sNS1 proteins modulated the capacity for BMDCs to induce specific T-cell responses as indicated by reduced IFN-γ levels in both CD4 + and CD8 + T cells after BMDC co-cultivation. In human monocyte-derived DCs, poly(I:C)-induced upregulation of co-stimulatory molecules and cytokine responses were even more strongly impaired by TBEV sNS1 or WNV sNS1 pretreatment than in the murine system. Our findings indicate that exogenous flaviviral sNS1 proteins interfere with DC-mediated stimulation of T cells, which is crucial for the initiation of cell-mediated adaptive immune responses in human flavivirus infections. Collectively, our data determine soluble flaviviral NS1 as a virulence factor responsible for a dampened immune response to flavivirus infections. IMPORTANCE The effective initiation of protective host immune responses controls the outcome of infection, and dysfunctional T-cell responses have previously been associated with symptomatic human flavivirus infections. We demonstrate that secreted flavivirus NS1 proteins modulate innate immune responses of uninfected bystander cells. In particular, sNS1 markedly reduced the capacity of dendritic cells to stimulate T-cell responses upon activation. Hence, by modulating cellular host responses that are required for effective antigen presentation and initiation of adaptive immunity, sNS1 proteins may contribute to severe outcomes of flavivirus disease.

Published
2023
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49. CLEC12A Binds to Legionella pneumophila but Has No Impact on the Host's Antibacterial Response.

Author

Klatt AB, Diersing C, Lippmann J, Mayer-Lambertz S, Stegmann F, Fischer S, Caesar S, Fiocca Vernengo F, Hönzke K, Hocke AC, Ruland J, Witzenrath M, Lepenies B, and Opitz B

Subjects
Animals, Humans, Mice, Macrophages metabolism, Macrophages, Alveolar metabolism, Immunity, Innate, Lectins, C-Type metabolism, Legionella pneumophila immunology, Legionnaires' Disease immunology, Legionnaires' Disease microbiology, Receptors, Mitogen immunology, Host-Pathogen Interactions
Abstract

Legionella pneumophila is an intracellular pathogen that can cause severe pneumonia after the inhalation of contaminated aerosols and replication in alveolar macrophages. Several pattern recognition receptors (PRRs) have been identified that contribute to the recognition of L. pneumophila by the innate immune system. However, the function of the C-type lectin receptors (CLRs), which are mainly expressed by macrophages and other myeloid cells, remains largely unexplored. Here, we used a library of CLR-Fc fusion proteins to search for CLRs that can bind the bacterium and identified the specific binding of CLEC12A to L. pneumophila . Subsequent infection experiments in human and murine macrophages, however, did not provide evidence for a substantial role of CLEC12A in controlling innate immune responses to the bacterium. Consistently, antibacterial and inflammatory responses to Legionella lung infection were not significantly influenced by CLEC12A deficiency. Collectively, CLEC12A is able to bind to L. pneumophila -derived ligands but does not appear to play a major role in the innate defense against L. pneumophila .

Published
2023
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50. The Vi Capsular Polysaccharide of Salmonella Typhi Promotes Macrophage Phagocytosis by Binding the Human C-Type Lectin DC-SIGN.

Author

Zhang LF, Lepenies B, Nakamae S, Young BM, Santos RL, Raffatellu M, Cobb BA, Hiyoshi H, and Bäumler AJ

Subjects
Humans, Polysaccharides, Bacterial metabolism, Lectins, C-Type metabolism, Phagocytosis, Macrophages metabolism, Salmonella typhi, Typhoid Fever microbiology
Abstract

Capsular polysaccharides are common virulence factors of extracellular, but not intracellular bacterial pathogens, due to the antiphagocytic properties of these surface structures. It is therefore paradoxical that Salmonella enterica subspecies enterica serovar Typhi, an intracellular pathogen, synthesizes a virulence-associated (Vi) capsule, which exhibits antiphagocytic properties. Here, we show that the Vi capsular polysaccharide has different functions when S. Typhi interacts with distinct subsets of host phagocytes. The Vi capsular polysaccharide allowed S. Typhi to selectively evade phagocytosis by human neutrophils while promoting human macrophage phagocytosis. A screen of C-type lectin receptors identified human DC-SIGN as the receptor involved in macrophage binding and phagocytosis of capsulated S. Typhi. Consistent with the anti-inflammatory activity of DC-SIGN, purified Vi capsular polysaccharide reduced inflammatory responses in macrophages. These data suggest that binding of the human C-type lectin receptor DC-SIGN by the Vi capsular polysaccharide contributes to the pathogenesis of typhoid fever. IMPORTANCE Salmonella enterica subspecies enterica serovar Typhi is the causative agent of typhoid fever. The recent emergence of S. Typhi strains which are resistant to antibiotic therapy highlights the importance of vaccination in managing typhoid fever. The virulence-associated (Vi) capsular polysaccharide is an effective vaccine against typhoid fever, but the role the capsule plays during pathogenesis remains incompletely understood. Here, we identify the human C-type lectin receptor DC-SIGN as the receptor for the Vi capsular polysaccharide. Binding of capsulated S. Typhi to DC-SIGN resulted in phagocytosis of the pathogen by macrophages and induction of an anti-inflammatory cytokine response. Thus, the interaction of the Vi capsular polysaccharide with human DC-SIGN contributes to the pathogenesis of typhoid fever and should be further investigated in the context of vaccine development.

Published
2022
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