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1. Root responses to aluminium and iron stresses require the SIZ1 SUMO ligase to modulate the STOP1 transcription factor.

Author

Mercier, Caroline, Roux, Brice, Have, Marien, Le Poder, Léa, Duong, Nathalie, David, Pascale, Leonhardt, Nathalie, Blanchard, Laurence, Naumann, Christin, Abel, Steffen, Cuyas, Laura, Pluchon, Sylvain, Nussaume, Laurent, and Desnos, Thierry

Subjects
ALMT1, Arabidopsis, SIZ1, STOP1, SUMO, aluminium, iron, phosphate, root, Aluminum, Arabidopsis, Arabidopsis Proteins, Gene Expression Regulation, Plant, Iron, Ligases, Mutation, Plant Roots, Signal Transduction, Stress, Physiological, Sumoylation, Transcription Factors
Abstract

STOP1, an Arabidopsis transcription factor favouring root growth tolerance against Al toxicity, acts in the response to iron under low Pi (-Pi). Previous studies have shown that Al and Fe regulate the stability and accumulation of STOP1 in roots, and that the STOP1 protein is sumoylated by an unknown E3 ligase. Here, using a forward genetics suppressor screen, we identified the E3 SUMO (small ubiquitin-like modifier) ligase SIZ1 as a modulator of STOP1 signalling. Mutations in SIZ1 increase the expression of ALMT1 (a direct target of STOP1) and root growth responses to Al and Fe stress in a STOP1-dependent manner. Moreover, loss-of-function mutations in SIZ1 enhance the abundance of STOP1 in the root tip. However, no sumoylated STOP1 protein was detected by Western blot analysis in our sumoylation assay in Escherichia coli, suggesting the presence of a more sophisticated mechanism. We conclude that the sumo ligase SIZ1 negatively regulates STOP1 signalling, at least in part by modulating STOP1 protein in the root tip. Our results will allow a better understanding of this signalling pathway.

Published
2021

2. AtMRP6/AtABCC6, an ATP-Binding Cassette transporter gene expressed during early steps of seedling development and up-regulated by cadmium in Arabidopsis thaliana

Author

Jacquet Hélène, Gaillard Stéphane, Vavasseur Alain, Leonhardt Nathalie, and Forestier Cyrille

Subjects
Botany, QK1-989
Abstract

Abstract Background ABC proteins constitute one of the largest families of transporters found in all living organisms. In Arabidopsis thaliana, 120 genes encoding ABC transporters have been identified. Here, the characterization of one member of the MRP subclass, AtMRP6, is described. Results This gene, located on chromosome 3, is bordered by AtMRP3 and AtMRP7. Using real-time quantitative PCR (RT-Q-PCR) and the GUS reporter gene, we found that this gene is essentially expressed during early seedling development, in the apical meristem and at initiation point of secondary roots, especially in xylem-opposite pericycle cells where lateral roots initiate. The level of expression of AtMRP6 in response to various stresses was explored and a significant up-regulation after cadmium (Cd) treatment was detected. Among the three T-DNA insertion lines available from the Salk Institute library, two knock-out mutants, Atmrp6.1 and Atmrp6.2 were invalidated for the AtMRP6 gene. In the presence of Cd, development of leaves was more affected in the mutants than wild-type plants, whereas root elongation and ramification was comparable. Conclusion The position of AtMRP6 on chromosome 3, flanked by two other MRP genes, (all of which being induced by Cd) suggests that AtMRP6 is part of a cluster involved in metal tolerance, although additional functions in planta cannot be discarded.

Published
2008
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3. Isolation of a strong Arabidopsis guard cell promoter and its potential as a research tool

Author

Siegel Robert S, Leonhardt Nathalie, Costa Alex, Yang Yingzhen, and Schroeder Julian I

Subjects
Plant culture, SB1-1110, Biology (General), QH301-705.5
Abstract

Abstract Background A common limitation in guard cell signaling research is that it is difficult to obtain consistent high expression of transgenes of interest in Arabidopsis guard cells using known guard cell promoters or the constitutive 35S cauliflower mosaic virus promoter. An additional drawback of the 35S promoter is that ectopically expressing a gene throughout the organism could cause pleiotropic effects. To improve available methods for targeted gene expression in guard cells, we isolated strong guard cell promoter candidates based on new guard cell-specific microarray analyses of 23,000 genes that are made available together with this report. Results A promoter, pGC1(At1g22690), drove strong and relatively specific reporter gene expression in guard cells including GUS (beta-glucuronidase) and yellow cameleon YC3.60 (GFP-based calcium FRET reporter). Reporter gene expression was weaker in immature guard cells. The expression of YC3.60 was sufficiently strong to image intracellular Ca2+ dynamics in guard cells of intact plants and resolved spontaneous calcium transients in guard cells. The GC1 promoter also mediated strong reporter expression in clustered stomata in the stomatal development mutant too-many-mouths (tmm). Furthermore, the same promoter::reporter constructs also drove guard cell specific reporter expression in tobacco, illustrating the potential of this promoter as a method for high level expression in guard cells. A serial deletion of the promoter defined a guard cell expression promoter region. In addition, anti-sense repression using pGC1 was powerful for reducing specific GFP gene expression in guard cells while expression in leaf epidermal cells was not repressed, demonstrating strong cell-type preferential gene repression. Conclusion The pGC1 promoter described here drives strong reporter expression in guard cells of Arabidopsis and tobacco plants. It provides a potent research tool for targeted guard cell expression or gene silencing. It is also applicable to reduce specific gene expression in guard cells, providing a method for circumvention of limitations arising from genetic redundancy and lethality. These advances could be very useful for manipulating signaling pathways in guard cells and modifying plant performance under stress conditions. In addition, new guard cell and mesophyll cell-specific 23,000 gene microarray data are made publicly available here.

Published
2008
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5. Arabidopsis hydathodes are sites of auxin accumulation and nutrient scavenging.

Author

Routaboul, Jean‐Marc, Bellenot, Caroline, Olympio, Aurore, Clément, Gilles, Citerne, Sylvie, Remblière, Céline, Charvin, Magali, Franke, Lars, Chiarenza, Serge, Vasselon, Damien, Jardinaud, Marie‐Françoise, Carrère, Sébastien, Nussaume, Laurent, Laufs, Patrick, Leonhardt, Nathalie, Navarro, Lionel, Schattat, Martin, and Noël, Laurent D.

Subjects
PLANT cell walls, LEAF anatomy, VASCULAR plants, GENE expression, AUXIN
Abstract

SUMMARY: Hydathodes are small organs found on the leaf margins of vascular plants which release excess xylem sap through a process called guttation. While previous studies have hinted at additional functions of hydathode in metabolite transport or auxin metabolism, experimental support is limited. We conducted comprehensive transcriptomic, metabolomic and physiological analyses of mature Arabidopsis hydathodes. This study identified 1460 genes differentially expressed in hydathodes compared to leaf blades, indicating higher expression of most genes associated with auxin metabolism, metabolite transport, stress response, DNA, RNA or microRNA processes, plant cell wall dynamics and wax metabolism. Notably, we observed differential expression of genes encoding auxin‐related transcriptional regulators, biosynthetic processes, transport and vacuolar storage supported by the measured accumulation of free and conjugated auxin in hydathodes. We also showed that 78% of the total content of 52 xylem metabolites was removed from guttation fluid at hydathodes. We demonstrate that NRT2.1 and PHT1;4 transporters capture nitrate and inorganic phosphate in guttation fluid, respectively, thus limiting the loss of nutrients during this process. Our transcriptomic and metabolomic analyses unveil an organ with its specific physiological and biological identity. Significance Statement: Hydathodes are vascular discontinuities at leaf margins of all vascular plants which allow the release of excess water during a process known as guttation. The physiology of this organ is poorly characterised. We conducted comprehensive transcriptomic, metabolomic and physiological analyses of mature hydathodes of Arabidopsis and demonstrated that those organs are sites of auxin accumulation and active nutrient scavenging. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Cesium Translocation in Rice

Author

Tanoi, Keitaro, Nobori, Tatsuya, Shiomi, Shuto, Saito, Takumi, Kobayashi, Natsuko I., Leonhardt, Nathalie, Nakanishi, Tomoko M., Nakanishi, Tomoko M., editor, O`Brien, Martin, editor, and Tanoi, Keitaro, editor

Published
2019
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7. PlantACT! - how to tackle the climate crisis

Author

King Abdullah University of Science and Technology, European Commission, Hirt, Heribert [0000-0003-3119-9633], Al-Babili, Salim [0000-0003-4823-2882], Almeida-Trapp, Marilia [0000-0002-9980-322X], Aranda, Manuel [0000-0001-6673-016X], Bartels, D. [0000-0003-0505-3083], Bennett, Malcom [0000-0003-0475-390X], Blilou, Ikram [0000-0001-8003-3782], Boer, Damian [0000-0003-1240-2253], Boulouis, Alix [0000-0002-2552-3938], Bowler, Chris [0000-0003-3835-6187], Brunel-Muguet, Sophie [0000-0001-6940-4618], Chardon, Fabien [0000-0001-7909-3884], Colcombet, Jean [0000-0002-0176-3857], Colot, Vincent [0000-0002-6382-1610], Daszkowska-Golec, Agata [0000-0001-9479-0813], Dinneny, Jose R. [0000-0002-3998-724X], Field, Ben [0000-0003-2142-4606], Gardener, Catherine H. [0000-0001-8528-3772], Gojon, Alain [0000-0001-5412-8606], Gomès, Eric [0000-0003-2582-1203], Gomez-Alvarez, Eva María [0000-0002-1284-7212], Gutiérrez Armenta, Crisanto [0000-0001-8905-8222], Havaux, Michel [0000-0002-6434-393X], Hayes, Scott [0000-0001-8943-6238], Heard, Edith [0000-0001-8052-7117], Alghamdi, Amal Khalaf [0000-0002-3491-5271], Laplaze, Laurent [0000-0002-6568-6504], Lauersen, Kyle J. [0000-0002-5538-7201], Leonhardt, Nathalie [0000-0002-5392-9732], Johnson, Xenie [0000-0003-3424-7047], Jones, Jonathan [0000-0002-4953-261X], Kollist, Hannes [0000-0002-6895-3583], Kopriva, Stanislav [0000-0002-7416-6551], Krapp, Anne [0000-0003-2034-5615], Masson, Mauricio Lopez-Portillo [0000-0003-3895-6254], McCabe, Matthew F. [0000-0002-1279-5272], Merendino, Livia [0000-0003-1593-5442], Molina, Antonio [0000-0003-3137-7938], Moreno Ramirez, Jose L. [0000-0002-0907-1361], Mueller-Roeber, Bernd [0000-0002-1410-464X], Nicolas, Michael [0000-0002-7311-5306], Nir, Ido [0000-0002-6223-6700], Pardo, José M. [0000-0003-4510-8624], Reichheld, Jean-Philippe [0000-0001-6884-0602], Rodriguez, Pedro [0000-0002-5886-9425], Rouached, Hatem [0000-0002-7751-0477], Saad, Maged M. [0000-0002-5655-8674], Schlögelhofer, Peter [0000-0002-0909-3587], De Smet, Ive [0000-0003-4607-8893], Stanschewski, Clara [0000-0002-3596-9108], Stra, Alice [0000-0002-0856-6039], Tester, Mark [0000-0002-5085-8801], Weber, A. [0000-0003-0970-4672], Weigel, Detlef [0000-0002-2114-7963], Wigge, Philip [0000-0003-4822-361X], Wrzaczek, Michael [0000-0002-5946-9060], Wulff, Brande B.H. [0000-0003-4044-4346], Hirt, Heribert, Al-Babili, Salim, Almeida-Trapp, Marilia, Martin, Antoine, Aranda, Manuel, Bartels, D., Bennett, Malcom, Blilou, Ikram, Boer, Damian, Boulouis, Alix, Bowler, Chris, Brunel-Muguet, Sophie, Chardon, Fabien, Colcombet, Jean, Colot, Vincent, Daszkowska-Golec, Agata, Dinneny, Jose R., Field, Ben, Froehlich, Katja, Gardener, Catherine H., Gojon, Alain, Gomès, Eric, Gomez-Alvarez, Eva María, Gutiérrez Armenta, Crisanto, Havaux, Michel, Hayes, Scott, Heard, Edith, Hodges, Michael, Alghamdi, Amal Khalaf, Laplaze, Laurent, Lauersen, Kyle J., Leonhardt, Nathalie, Johnson, Xenie, Jones, Jonathan, Kollist, Hannes, Kopriva, Stanislav, Krapp, Anne, Masson, Mauricio Lopez-Portillo, McCabe, Matthew F., Merendino, Livia, Molina, Antonio, Moreno Ramirez, Jose L., Mueller-Roeber, Bernd, Nicolas, Michael, Nir, Ido, Orduna, Izamar Olivas, Pardo, José M., Reichheld, Jean-Philippe, Rodriguez, Pedro, Rouached, Hatem, Saad, Maged M., Schlögelhofer, Peter, Singh, Kirti A., De Smet, Ive, Stanschewski, Clara, Stra, Alice, Tester, Mark, Walsh, Catherine, Weber, A., Weigel, Detlef, Wigge, Philip, Wrzaczek, Michael, Wulff, Brande B.H., Young, Iain M., King Abdullah University of Science and Technology, European Commission, Hirt, Heribert [0000-0003-3119-9633], Al-Babili, Salim [0000-0003-4823-2882], Almeida-Trapp, Marilia [0000-0002-9980-322X], Aranda, Manuel [0000-0001-6673-016X], Bartels, D. [0000-0003-0505-3083], Bennett, Malcom [0000-0003-0475-390X], Blilou, Ikram [0000-0001-8003-3782], Boer, Damian [0000-0003-1240-2253], Boulouis, Alix [0000-0002-2552-3938], Bowler, Chris [0000-0003-3835-6187], Brunel-Muguet, Sophie [0000-0001-6940-4618], Chardon, Fabien [0000-0001-7909-3884], Colcombet, Jean [0000-0002-0176-3857], Colot, Vincent [0000-0002-6382-1610], Daszkowska-Golec, Agata [0000-0001-9479-0813], Dinneny, Jose R. [0000-0002-3998-724X], Field, Ben [0000-0003-2142-4606], Gardener, Catherine H. [0000-0001-8528-3772], Gojon, Alain [0000-0001-5412-8606], Gomès, Eric [0000-0003-2582-1203], Gomez-Alvarez, Eva María [0000-0002-1284-7212], Gutiérrez Armenta, Crisanto [0000-0001-8905-8222], Havaux, Michel [0000-0002-6434-393X], Hayes, Scott [0000-0001-8943-6238], Heard, Edith [0000-0001-8052-7117], Alghamdi, Amal Khalaf [0000-0002-3491-5271], Laplaze, Laurent [0000-0002-6568-6504], Lauersen, Kyle J. [0000-0002-5538-7201], Leonhardt, Nathalie [0000-0002-5392-9732], Johnson, Xenie [0000-0003-3424-7047], Jones, Jonathan [0000-0002-4953-261X], Kollist, Hannes [0000-0002-6895-3583], Kopriva, Stanislav [0000-0002-7416-6551], Krapp, Anne [0000-0003-2034-5615], Masson, Mauricio Lopez-Portillo [0000-0003-3895-6254], McCabe, Matthew F. [0000-0002-1279-5272], Merendino, Livia [0000-0003-1593-5442], Molina, Antonio [0000-0003-3137-7938], Moreno Ramirez, Jose L. [0000-0002-0907-1361], Mueller-Roeber, Bernd [0000-0002-1410-464X], Nicolas, Michael [0000-0002-7311-5306], Nir, Ido [0000-0002-6223-6700], Pardo, José M. [0000-0003-4510-8624], Reichheld, Jean-Philippe [0000-0001-6884-0602], Rodriguez, Pedro [0000-0002-5886-9425], Rouached, Hatem [0000-0002-7751-0477], Saad, Maged M. [0000-0002-5655-8674], Schlögelhofer, Peter [0000-0002-0909-3587], De Smet, Ive [0000-0003-4607-8893], Stanschewski, Clara [0000-0002-3596-9108], Stra, Alice [0000-0002-0856-6039], Tester, Mark [0000-0002-5085-8801], Weber, A. [0000-0003-0970-4672], Weigel, Detlef [0000-0002-2114-7963], Wigge, Philip [0000-0003-4822-361X], Wrzaczek, Michael [0000-0002-5946-9060], Wulff, Brande B.H. [0000-0003-4044-4346], Hirt, Heribert, Al-Babili, Salim, Almeida-Trapp, Marilia, Martin, Antoine, Aranda, Manuel, Bartels, D., Bennett, Malcom, Blilou, Ikram, Boer, Damian, Boulouis, Alix, Bowler, Chris, Brunel-Muguet, Sophie, Chardon, Fabien, Colcombet, Jean, Colot, Vincent, Daszkowska-Golec, Agata, Dinneny, Jose R., Field, Ben, Froehlich, Katja, Gardener, Catherine H., Gojon, Alain, Gomès, Eric, Gomez-Alvarez, Eva María, Gutiérrez Armenta, Crisanto, Havaux, Michel, Hayes, Scott, Heard, Edith, Hodges, Michael, Alghamdi, Amal Khalaf, Laplaze, Laurent, Lauersen, Kyle J., Leonhardt, Nathalie, Johnson, Xenie, Jones, Jonathan, Kollist, Hannes, Kopriva, Stanislav, Krapp, Anne, Masson, Mauricio Lopez-Portillo, McCabe, Matthew F., Merendino, Livia, Molina, Antonio, Moreno Ramirez, Jose L., Mueller-Roeber, Bernd, Nicolas, Michael, Nir, Ido, Orduna, Izamar Olivas, Pardo, José M., Reichheld, Jean-Philippe, Rodriguez, Pedro, Rouached, Hatem, Saad, Maged M., Schlögelhofer, Peter, Singh, Kirti A., De Smet, Ive, Stanschewski, Clara, Stra, Alice, Tester, Mark, Walsh, Catherine, Weber, A., Weigel, Detlef, Wigge, Philip, Wrzaczek, Michael, Wulff, Brande B.H., and Young, Iain M.

Abstract

Greenhouse gas (GHG) emissions have created a global climate crisis which requires immediate interventions to mitigate the negative effects on all aspects of life on this planet. As current agriculture and land use contributes up to 25% of total GHG emissions, plant scientists take center stage in finding possible solutions for a transition to sustainable agriculture and land use. In this article, the PlantACT! (Plants for climate ACTion!) initiative of plant scientists lays out a road map of how and in which areas plant scientists can contribute to finding immediate, mid-term, and long-term solutions, and what changes are necessary to implement these solutions at the personal, institutional, and funding levels.

Published
2023

15. A conserved signaling axis integrates conflicting environmental drought and heat signals to control stomatal aperture in plants

Author

De Smet, Ive, primary, Xu, Xiangyu, additional, Praat, Myrthe, additional, Pizzio, Gaston, additional, Jiang, Zhang, additional, Driever, Steven, additional, Wang, Ren, additional, de Cotte, Brigitte van, additional, Villers, Selwyn, additional, Gevaert, Kris, additional, LEONHARDT, Nathalie, additional, Nelissen, Hilde, additional, Kinoshita, Toshinori, additional, Vanneste, Steffen, additional, Rodriguez, Pedro, additional, Zanten, Martijn van, additional, and Vu, Lam, additional

Published
2023
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20. PlantACT! – how to tackle the climate crisis

Author

Hirt, Heribert, primary, Al-Babili, Salim, additional, Almeida-Trapp, Marilia, additional, Martin, Antoine, additional, Aranda, Manuel, additional, Bartels, Dorothea, additional, Bennett, Malcolm, additional, Blilou, Ikram, additional, Boer, Damian, additional, Boulouis, Alix, additional, Bowler, Chris, additional, Brunel-Muguet, Sophie, additional, Chardon, Fabien, additional, Colcombet, Jean, additional, Colot, Vincent, additional, Daszkowska-Golec, Agata, additional, Dinneny, Jose R., additional, Field, Ben, additional, Froehlich, Katja, additional, Gardener, Catherine H., additional, Gojon, Alain, additional, Gomès, Eric, additional, Gomez-Alvarez, Eva María, additional, Gutierrez, Crisanto, additional, Havaux, Michel, additional, Hayes, Scott, additional, Heard, Edith, additional, Hodges, Michael, additional, Alghamdi, Amal Khalaf, additional, Laplaze, Laurent, additional, Lauersen, Kyle J., additional, Leonhardt, Nathalie, additional, Johnson, Xenie, additional, Jones, Jonathan, additional, Kollist, Hannes, additional, Kopriva, Stanislav, additional, Krapp, Anne, additional, Masson, Mauricio Lopez-Portillo, additional, McCabe, Matthew F., additional, Merendino, Livia, additional, Molina, Antonio, additional, Moreno Ramirez, Jose L., additional, Mueller-Roeber, Bernd, additional, Nicolas, Michael, additional, Nir, Ido, additional, Orduna, Izamar Olivas, additional, Pardo, Jose M., additional, Reichheld, Jean-Philippe, additional, Rodriguez, Pedro L., additional, Rouached, Hatem, additional, Saad, Maged M., additional, Schlögelhofer, Peter, additional, Singh, Kirti A., additional, De Smet, Ive, additional, Stanschewski, Clara, additional, Stra, Alice, additional, Tester, Mark, additional, Walsh, Catherine, additional, Weber, Andreas P.M., additional, Weigel, Detlef, additional, Wigge, Philip, additional, Wrzaczek, Michael, additional, Wulff, Brande B.H., additional, and Young, Iain M., additional

Published
2023
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21. A conserved signaling axis integrates conicting environmental drought and heat signals to control stomatal aperture in plants

Author

De Smet, Ive, Praat, Myrthe, Pizzio, Gaston, Jiang, Zhang, Driever, Steven, Wang, Ren, Leonhardt, Nathalie, Kinoshita, Toshinori, Vanneste, Steffen, Rodriguez, Pedro, Van Zanten, Martijn, De Smet, Ive, Praat, Myrthe, Pizzio, Gaston, Jiang, Zhang, Driever, Steven, Wang, Ren, Leonhardt, Nathalie, Kinoshita, Toshinori, Vanneste, Steffen, Rodriguez, Pedro, and Van Zanten, Martijn

Abstract

Plants continuously respond to changing environmental conditions to prevent damage and maintain optimal performance. To regulate gas exchange with the environment and to control abiotic stress relief, plants have pores in their leaf epidermis, called stomata 1. Multiple environmental signals affect the opening and closing of these stomata 2. Heat promotes stomatal opening (to cool down) and drought induces stomatal closing (to prevent water loss). Coinciding stress conditions, however, may evoke conflicting stomatal responses, but the cellular mechanisms to resolve these conflicts are unknown. Here, we demonstrate that the high temperature-associated kinase TARGET OF TEMPERATURE 3 (TOT3) directly controls the activity of plasma membrane H+-ATPases to induce stomatal opening. This TOT3 activity is directly antagonized by OPEN STOMATA 1 (OST1), to prevent water loss during drought stress. This signaling axis harmonizes conflicting heat and drought signals to regulate stomatal aperture. In the context of global climate change, understanding how conflicting stress signals converge on stomatal regulation allows the development of climate change-ready crops.

Published
2023

22. A conserved signaling axis integrates conicting environmental drought and heat signals to control stomatal aperture in plants

Author

Sub Plant Stress Resilience, Plant Stress Resilience, De Smet, Ive, Praat, Myrthe, Pizzio, Gaston, Jiang, Zhang, Driever, Steven, Wang, Ren, Leonhardt, Nathalie, Kinoshita, Toshinori, Vanneste, Steffen, Rodriguez, Pedro, Van Zanten, Martijn, Sub Plant Stress Resilience, Plant Stress Resilience, De Smet, Ive, Praat, Myrthe, Pizzio, Gaston, Jiang, Zhang, Driever, Steven, Wang, Ren, Leonhardt, Nathalie, Kinoshita, Toshinori, Vanneste, Steffen, Rodriguez, Pedro, and Van Zanten, Martijn

Published
2023

23. Insight into physiological and biochemical determinants of salt stress tolerance in Tetraploid citrus

Author

Bonnin, Marie, Favreau, Bénédicte, Soriano, Alexandre, Leonhardt, Nathalie, Oustric, Julie, Lourkisti, Radia, Ollitrault, Patrick, Morillon, Raphaël, Berti, Liliane, Santini, Jérémie, Bonnin, Marie, Favreau, Bénédicte, Soriano, Alexandre, Leonhardt, Nathalie, Oustric, Julie, Lourkisti, Radia, Ollitrault, Patrick, Morillon, Raphaël, Berti, Liliane, and Santini, Jérémie

Abstract

Citrus are classified as salt-sensitive crops. However, a large diversity has been observed regarding the trends of tolerance among citrus. In the present article, physiological and biochemical studies of salt stress tolerance were carried out according to the level of polyploidy of different citrus genotypes. We particularly investigated the impact of tetraploidy in trifoliate orange (Poncirus trifoliata (L.) Raf.) (PO4x) and Cleopatra mandarin (Citrus reshni Hort. Ex Tan.) (CL4x) on the tolerance to salt stress compared to their respective diploids (PO2x and CL2x). Physiological parameters such as gas exchange, ions contents in leaves and roots were analyzed. Roots and leaves samples were collected to measure polyphenol, malondialdehyde (MDA), ascorbate and H2O2 contents but also to measure the activities of enzymes involved in the detoxification of active oxygen species (ROS). Under control conditions, the interaction between genotype and ploidy allowed to discriminate different behavior in terms of photosynthetic and antioxidant capacities. These results were significantly altered when salt stress was applied when salt stress was applied. Contrary to the most sensitive genotype, that is to say the diploid trifoliate orange PO2x, PO4x was able to maintain photosynthetic activity under salt stress and had better antioxidant capacities. The same observation was made regarding the CL4x genotype known to be more tolerant to salt stress. Our results showed that tetraploidy may be a factor that could enhance salt stress tolerance in citrus.

Published
2023

24. PlantACT! – how to tackle the climate crisis

Author

Hirt, Heribert, Al-Babili, Salim, Almeida-Trapp, Marilia, Martin, Antoine, Aranda, Manuel, Bartels, Dorothea, Bennett, Malcolm, Blilou, Ikram, Boer, Damian, Boulouis, Alix, Bowler, Chris, Brunel-Muguet, Sophie, Chardon, Fabien, Colcombet, Jean, Colot, Vincent, Daszkowska-Golec, Agata, Dinneny, Jose R., Field, Ben, Froehlich, Katja, Gardener, Catherine H., Gojon, Alain, Gomès, Eric, Gomez-Alvarez, Eva María, Gutierrez, Crisanto, Havaux, Michel, Hayes, Scott, Heard, Edith, Hodges, Michael, Alghamdi, Amal Khalaf, Laplaze, Laurent, Lauersen, Kyle J., Leonhardt, Nathalie, Johnson, Xenie, Jones, Jonathan, Kollist, Hannes, Kopriva, Stanislav, Krapp, Anne, Masson, Mauricio Lopez Portillo, McCabe, Matthew F., Merendino, Livia, Molina, Antonio, Moreno Ramirez, Jose L., Mueller-Roeber, Bernd, Nicolas, Michael, Nir, Ido, Orduna, Izamar Olivas, Pardo, Jose M., Reichheld, Jean Philippe, Rodriguez, Pedro L., Rouached, Hatem, Saad, Maged M., Schlögelhofer, Peter, Singh, Kirti A., De Smet, Ive, Stanschewski, Clara, Stra, Alice, Tester, Mark, Walsh, Catherine, Weber, Andreas P.M., Weigel, Detlef, Wigge, Philip, Wrzaczek, Michael, Wulff, Brande B.H., Young, Iain M., Hirt, Heribert, Al-Babili, Salim, Almeida-Trapp, Marilia, Martin, Antoine, Aranda, Manuel, Bartels, Dorothea, Bennett, Malcolm, Blilou, Ikram, Boer, Damian, Boulouis, Alix, Bowler, Chris, Brunel-Muguet, Sophie, Chardon, Fabien, Colcombet, Jean, Colot, Vincent, Daszkowska-Golec, Agata, Dinneny, Jose R., Field, Ben, Froehlich, Katja, Gardener, Catherine H., Gojon, Alain, Gomès, Eric, Gomez-Alvarez, Eva María, Gutierrez, Crisanto, Havaux, Michel, Hayes, Scott, Heard, Edith, Hodges, Michael, Alghamdi, Amal Khalaf, Laplaze, Laurent, Lauersen, Kyle J., Leonhardt, Nathalie, Johnson, Xenie, Jones, Jonathan, Kollist, Hannes, Kopriva, Stanislav, Krapp, Anne, Masson, Mauricio Lopez Portillo, McCabe, Matthew F., Merendino, Livia, Molina, Antonio, Moreno Ramirez, Jose L., Mueller-Roeber, Bernd, Nicolas, Michael, Nir, Ido, Orduna, Izamar Olivas, Pardo, Jose M., Reichheld, Jean Philippe, Rodriguez, Pedro L., Rouached, Hatem, Saad, Maged M., Schlögelhofer, Peter, Singh, Kirti A., De Smet, Ive, Stanschewski, Clara, Stra, Alice, Tester, Mark, Walsh, Catherine, Weber, Andreas P.M., Weigel, Detlef, Wigge, Philip, Wrzaczek, Michael, Wulff, Brande B.H., and Young, Iain M.

Abstract

Greenhouse gas (GHG) emissions have created a global climate crisis which requires immediate interventions to mitigate the negative effects on all aspects of life on this planet. As current agriculture and land use contributes up to 25% of total GHG emissions, plant scientists take center stage in finding possible solutions for a transition to sustainable agriculture and land use. In this article, the PlantACT! (Plants for climate ACTion!) initiative of plant scientists lays out a road map of how and in which areas plant scientists can contribute to finding immediate, mid-term, and long-term solutions, and what changes are necessary to implement these solutions at the personal, institutional, and funding levels.

Published
2023

26. Xylem K+ loading modulates K+ and Cs+ absorption and distribution in Arabidopsis under K+-limited conditions.

Author

Satomi Kanno, Martin, Ludovic, Vallier, Natacha, Chiarenza, Serge, Tatsuya Nobori, Furukawa, Jun, Nussaume, Laurent, Vavasseur, Alain, and Leonhardt, Nathalie

Subjects
PLANT translocation, CESIUM, XYLEM, PLANT cells & tissues, ARABIDOPSIS, GENETIC transcription regulation, STABLE isotopes
Abstract

Potassium (K+) is an essential macronutrient for plant growth. The transcriptional regulation of K+ transporter genes is one of the key mechanisms by which plants respond to K+ deficiency. Among the HAK/KUP/KT transporter family, HAK5, a high-affinity K+ transporter, is essential for root K+ uptake under low external K+ conditions. HAK5 expression in the root is highly induced by low external K+ concentration. While the molecular mechanisms of HAK5 regulation have been extensively studied, it remains unclear how plants sense and coordinates K+ uptake and translocation in response to changing environmental conditions. Using skor mutants, which have a defect in root-to-shoot K+translocation, we have been able to determine how the internal K+ status affects the expression of HAK5. In skor mutant roots, under K+ deficiency, HAK5 expression was lower than in wild-type although the K+ concentration in roots was not significantly different. These results reveal that HAK5 is not only regulated by external K+ conditions but it is also regulated by internal K+ levels, which is in agreement with recent findings. Additionally, HAK5 plays a major role in the uptake of Cs+ in roots. Therefore, studying Cs+in roots and having more detailed information about its uptake and translocation in the plant would be valuable. Radioactive tracing experiments revealed not only a reduction in the uptake of 137Cs+ and 42K+ in skor mutants compared to wild-type but also a different distribution of 137Cs+ and 42K+ in tissues. In order to gain insight into the translocation, accumulation, and repartitioning of both K+and Cs+ in plants, long-term treatment and split root experiments were conducted with the stable isotopes 133Cs+ and 85Rb+. Finally, our findings show that the K+ distribution in plant tissues regulates root uptake of K+ and Cs+ similarly, depending on HAK5; however, the translocation and accumulation of the two elements are different. [ABSTRACT FROM AUTHOR]

Published
2023
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27. Arabidopsis hydathodes are sites of intense auxin metabolism and nutrient scavenging

Author

Routaboul, Jean-Marc, primary, Bellenot, Caroline, additional, Clément, Gilles, additional, Citerne, Sylvie, additional, Remblière, Céline, additional, Charvin, Magali, additional, Franke, Lars, additional, Chiarenza, Serge, additional, Vasselon, Damien, additional, Jardinaud, Marie-Françoise, additional, Carrère, Sébastien, additional, Nussaume, Laurent, additional, Laufs, Patrick, additional, Leonhardt, Nathalie, additional, Navarro, Lionel, additional, Schattat, Martin, additional, and Noël, Laurent D., additional

Published
2022
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47. AtHMA3, a [P.sub.1B]-ATPase allowing Cd/Zn/Co/Pb vacuolar storage in Arabidopsis

Author

Morel, Melanie, Crouzet, Jereme, Gravot, Antoine, Auroy, Pascaline, Leonhardt, Nathalie, Vavasseur, Alain, and Richaud, Pierre

Subjects
Adenosine triphosphatase -- Research, Cobalt -- Research, Arabidopsis thaliana -- Physiological aspects, Arabidopsis thaliana -- Genetic aspects, Vacuoles -- Research, Biological sciences, Science and technology
Published
2009

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