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A panel of monoclonal antibodies was used in an avidinbiotin immunoperoxidase technique to provide an immunohistologic analysis of the inflammatory infiltrates in the gingiva of osseointegrated implants. A total of 27 gingival and 13 interimplant specimens was obtained from 13 patients. Eighteen of the gingival specimens were clinically healthy, while nine showed overt signs of clinical inflammation (GI = 2 or 3). All specimens showed some degree of inflammation histologically, although the size of the inflammatory infiltrate was much greater in the clinically inflamed specimens. There was no significant difference in the proportion of T (50% to 60%) or B (40% to 50%) lymphocytes in either group. The CD4:CD8 ratio also showed no significant difference: 1.6:1 in the healthy group and 2.0 in the inflamed group. Similar ratios were also found in the infiltrates of the interimplant tissue. The immunohistologic analysis suggests that the gingival lesion associated with these implants is a stable, well-controlled response. [ABSTRACT FROM AUTHOR]

In the last few years, particular focus has been devoted to the life cycle performance of fastening systems, which is reflected in increasing numbers of publications, standards and large-scale research efforts. Simultaneously, experience shows that in many cases, where fastening systems are implemented – such as industrial facilities – the design of fasteners is governed by fatigue loading under dynamic characteristics. In order to perform an adequate design and to specify the most efficient and appropriate fastening product, the engineer needs to access and process a broad range of technical and commercial information. Building information modelling (BIM), as a data management method in the construction industry, can supply such information and accommodate a comprehensive design and specification process. Furthermore, the application of BIM-based processes, such as the generation of a BIM-model, allows to use the important information for the construction as well as the life cycle management with different actions and time dependencies of the asset and its components. As a consequence, the BIM model offers the potential to correlate different data relevant for achieving the goals of the respective application, in order to ensure a more effective and correct design of the fastening. This paper demonstrates such a BIM-based design framework for an Industry 4.0 case, and in particular, the installation of a factory robot through post-installed anchors under fatigue-relevant loading in concrete.

Close-in extrasolar giant planets are expected to cool their thermospheres by producing H+3 emission in the near-infrared (NIR), but simulations predict H+3 emission intensities that differ in the resulting intensity by several orders of magnitude. We want to test the observability of H+3 emission with CRIRES at the Very Large Telescope (VLT), providing adequate spectral resolution for planetary atmospheric lines in NIR spectra. We search for signatures of planetary H+3 emission in the L' band, using spectra of HD 209458 obtained during and after secondary eclipse of its transiting planet HD 209458 b. We searched for H+3 emission signatures in spectra containing the combined light of the star and, possibly, the planet. With the information on the ephemeris of the transiting planet, we derive the radial velocities at the time of observation and search for the emission at the expected line positions. We also apply a cross-correlation test to search for planetary signals and use a shift and add technique combining all observed spectra taken after secondary eclipse to calculate an upper emission limit. We do not find signatures of atmospheric H+3 emission in the spectra containing the combined light of HD 209458 and its orbiting planet. We calculate the emission limit for the H+3 line at 3953.0 nm [Q(1, 0)] to be 8.32 × 1018 W and a limit of 5.34 × 1018 W for the line at 3985.5 nm [Q(3, 0)]. Comparing our emission limits to the theoretical predictions suggests that we lack 1 to 3 magnitudes of sensitivity to measure H+3 emission in our target object. We show that under more favorable weather conditions the data quality can be improved significantly, reaching 5 × 1016 W for star-planet systems that are close to Earth. We estimate that pushing the detection limit down to 1015 W will be possible with ground-based observations with future instrumentation, for example, the European Extremly Large Telescope. [ABSTRACT FROM AUTHOR]

Graves' disease (GD) and Hashimoto's thyroiditis (HT) are the most common autoimmune thyroid diseases (AITD). MicroRNAs (miRNAs) critically control gene-expression and play an important role in regulating the immune response. The aim of this study was to prove significant variations of key immunoregulatory miRNAs in peripheral blood mononuclear cells (PBMCs) and in CD4 + and CD 8 + T-cells of AITD patients. Selected miRNAs were amplified by a semiquantitative SYBR Green PCR from PBMCs and purified CD4 + and CD 8 + T-cells of 59 patients with GD, HT, and healthy controls. Both GD and HT showed significantly decreased miRNA 200a_1 and miRNA 200a2" in CD4 + -T-cells (mean relative expression 12,57 in HT vs. 19.40 in control group (CG), p = 0.0002; 12,10 in GD vs. 19.40 in CG, p = 0.0002) and in CD8 + -T-cells (13.13 in HT vs. 18,12 in CG, p = 0.02; 11.66 in GD vs. 18.12 in CG, p = 0.0002). GD and HT showed significantly decreased miRNA 155_2 and miRNA 155"_1 in HT in CD8 + -T-cells (10.69 in HT vs. 11.30 in CG, p = 0.01; 10.40 in GD vs. 11.30 in CG, p = 0.005). This study confirms significant variations of miRNA200a and miRNA155 in patients suffering from GD and HT in vivo in CD4 + T-cells and CD8 + T-cells. These data may help to better understand the gene regulations in the causative cells causing these autoimmune processes. They extend our very limited knowledge concerning miRNAs in thyroid diseases. [ABSTRACT FROM AUTHOR]