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1. Modulation of Bronchial Epithelial Barrier Integrity by Low Molecular Weight Components from Birch Pollen.

Author

Sudharson, Srinidhi, Kalic, Tanja, Eckl-Dorna, Julia, Lengger, Nina, Breiteneder, Heimo, and Hafner, Christine

Subjects
MOLECULAR weights, POLLEN, EPITHELIAL cells, CELL analysis, BIRCH
Abstract

Pollen, in addition to allergens, comprise low molecular weight components (LMC) smaller than 3 kDa. Emerging evidence indicates the relevance of LMC in allergic immune responses. However, the interaction of birch pollen (BP)-derived LMC and epithelial cells has not been extensively studied. We investigated epithelial barrier modifications induced by exposure to BP LMC, using the human bronchial epithelial cell line 16HBE14o-. Epithelial cell monolayers were apically exposed to the major BP allergen Bet v 1, aqueous BP extract or BP-derived LMC. Barrier integrity after the treatments was monitored by measuring transepithelial electrical resistance at regular intervals and by using the xCELLigence Real-Time Cell Analysis system. The polarized release of cytokines 24 h following treatment was measured using a multiplex immunoassay. Epithelial barrier integrity was significantly enhanced upon exposure to BP LMC. Moreover, BP LMC induced the repair of papain-mediated epithelial barrier damage. The apical release of CCL5 and TNF-α was significantly reduced after exposure to BP LMC, while the basolateral release of IL-6 significantly increased. In conclusion, the results of our study demonstrate that BP-derived LMC modify the physical and immunological properties of bronchial epithelial cells and thus regulate airway epithelial barrier responses. [ABSTRACT FROM AUTHOR]

Published
2024
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8. The major birch pollen allergen Bet v 1 induces different responses in dendritic cells of birch pollen allergic and healthy individuals

Author

Smole, Ursula, Radauer, Christian, Lengger, Nina, Svoboda, Martin, Rigby, Neil, Bublin, Merima, Gaier, Sonja, Hoffmann-Sommergruber, Karin, Jensen-Jarolim, Erika, Mechtcheriakova, Diana, and Breiteneder, Heimo

Subjects
Adult, Male, Transcription, Genetic, Science, Binding, Competitive, Monocytes, Th2 Cells, Hypersensitivity, Humans, Phosphorylation, Betula, Receptors, IgE, Dendritic Cells, Allergens, Antigens, Plant, Immunoglobulin E, Endocytosis, Enzyme Activation, Kinetics, Cross-Linking Reagents, Gene Expression Regulation, Case-Control Studies, Pollen, Medicine, Female, Mitogen-Activated Protein Kinases, Research Article
Abstract

Dendritic cells play a fundamental role in shaping the immune response to allergens. The events that lead to allergic sensitization or tolerance induction during the interaction of the major birch pollen allergen Bet v 1 and dendritic cells are not very well studied. Here, we analyzed the uptake of Bet v 1 and the cross-reactive celery allergen Api g 1 by immature monocyte-derived dendritic cells (iMoDCs) of allergic and normal donors. In addition, we characterized the allergen-triggered intracellular signaling and transcriptional events. Uptake kinetics, competitive binding, and internalization pathways of labeled allergens by iMoDCs were visualized by live-cell imaging. Surface-bound IgE was detected by immunofluorescence microscopy and flow cytometry. Allergen- and IgE-induced gene expression of early growth response genes and Th1 and Th2 related cytokines and chemokines were analyzed by real-time PCR. Phosporylation of signaling kinases was analyzed by Western blot. Internalization of Bet v 1 by iMoDCs of both donor groups, likely by receptor-mediated caveolar endocytosis, followed similar kinetics. Bet v 1 outcompeted Api g 1 in cell surface binding and uptake. MoDCs of allergic and healthy donors displayed surface-bound IgE and showed a pronounced upregulation of Th2 cytokine- and NFκB-dependent genes upon non-specific Fcε receptor cross-linking. In contrast to these IgE-mediated responses, Bet v 1-stimulation increased transcript levels of the Th2 cytokines IL-4 and IL-13 but not of NFκB-related genes in MoDCs of BP allergic donors. Cells of healthy donors were either unresponsive or showed elevated mRNA levels of Th1-promoting chemokines. Moreover, Bet v 1 was able to induce Erk1/2 and p38 MAPK activation in BP allergics but only a slight p38 activation in normal donors. In conclusion, our data indicate that Bet v 1 favors the activation of a Th2 program only in DCs of BP allergic individuals.

Published
2015

9. A Cross-Reactive Human Single-Chain Antibody for Detection of Major Fish Allergens, Parvalbumins, and Identification of a Major IgE-Binding Epitope

Author

Bublin, Merima, Kostadinova, Maria, Fuchs, Julian E., Ackerbauer, Daniela, Moraes, Adolfo H., Almeida, Fabio C. L., Lengger, Nina, Hafner, Christine, Ebner, Christof, Radauer, Christian, Liedl, Klaus R., Valente, Ana Paula, and Breiteneder, Heimo

Subjects
musculoskeletal, neural, and ocular physiology, lcsh:R, Fishes, lcsh:Medicine, Allergens, Cross Reactions, Immunoglobulin E, Epitopes, Parvalbumins, nervous system, mental disorders, Animals, Humans, lcsh:Q, lcsh:Science, Epitope Mapping, Food Hypersensitivity, Research Article, Single-Chain Antibodies
Abstract

Fish allergy is associated with moderate to severe IgE-mediated reactions to the calcium binding parvalbumins present in fish muscle. Allergy to multiple fish species is caused by parvalbumin-specific cross-reactive IgE recognizing conserved epitopes. In this study, we aimed to produce cross-reactive single chain variable fragment (scFv) antibodies for the detection of parvalbumins in fish extracts and the identification of IgE epitopes. Parvalbumin-specific phage clones were isolated from the human ETH-2 phage display library by three rounds of biopanning either against cod parvalbumin or by sequential biopanning against cod (Gad m 1), carp (Cyp c 1) and rainbow trout (Onc m 1) parvalbumins. While biopanning against Gad m 1 resulted in the selection of clones specific exclusively for Gad m 1, the second approach resulted in the selection of clones cross-reacting with all three parvalbumins. Two clones, scFv-gco9 recognizing all three parvalbumins, and scFv-goo8 recognizing only Gad m 1 were expressed in the E. coli non-suppressor strain HB2151 and purified from the periplasm. scFv-gco9 showed highly selective binding to parvalbumins in processed fish products such as breaded cod sticks, fried carp and smoked trout in Western blots. In addition, the scFv-gco9-AP produced as alkaline phosphatase fusion protein, allowed a single-step detection of the parvalbumins. In competitive ELISA, scFv-gco9 was able to inhibit binding of IgE from fish allergic patients' sera to all three β-parvalbumins by up to 80%, whereas inhibition by scFv-goo8 was up to 20%. 1H/15N HSQC NMR analysis of the rGad m 1:scFv-gco9 complex showed participation of amino acid residues conserved among these three parvalbumins explaining their cross-reactivity on a molecular level. In this study, we have demonstrated an approach for the selection of cross-reactive parvalbumin-specific antibodies that can be used for allergen detection and for mapping of conserved epitopes.

Published
2015

10. 6th International Symposium on Molecular Allergology (ISMA)

Author

Hilger, Christiane, primary, Swiontek, Kyra, additional, Fischer, Jörg, additional, Hentges, François, additional, Lehners, Christiane, additional, Morisset, Martine, additional, Eberlein, Bernadette, additional, Biedermann, Tilo, additional, Ollert, Markus, additional, Wildner, Sabrina, additional, Stemeseder, Teresa, additional, Freier, Regina, additional, Briza, Peter, additional, Lang, Roland, additional, Batanero, Eva, additional, Villalba, Mayte, additional, Lidholm, Jonas, additional, Hawranek, Thomas, additional, Ferreira, Fatima, additional, Brandstetter, Hans, additional, Gadermaier, Gabriele, additional, Moingeon, Philippe, additional, Groeme, Rachel, additional, Bouley, Julien, additional, Bordas, Véronique, additional, Le Mignon, Maxime, additional, Bussières, Laetitia, additional, Lautrette, Aurélie, additional, Mascarell, Laurent, additional, Lombardi, Vincent, additional, Baron-Bodo, Véronique, additional, Chabre, Henri, additional, Batard, Thierry, additional, Nony, Emmanuel, additional, De Amicis, Karine Marafigo, additional, Watanabe, Alexandra Sayuri, additional, Figo, Daniele Danella, additional, Dos Santos-Pinto, José Roberto Aparecido, additional, Palma, Mario Sergio, additional, Castro, Fabio Fernandes Morato, additional, Kalil, Jorge, additional, Wohlschlager, Therese, additional, Ferreira-Briza, Fatima, additional, Santos, Keity Souza, additional, Faber, Margaretha, additional, Van Gasse, Athina, additional, Sabato, Vito, additional, Hagendorens, Margo M., additional, Bridts, Chris H., additional, De Clerck, Luc S., additional, Perales, Araceli Diaz, additional, Ebo, Didier, additional, Zavadakova, Petra, additional, Buchwalder, Aurélie, additional, Rebeaud, Fabien, additional, Märki, Iwan, additional, Gepp, Barbara, additional, Lengger, Nina, additional, Möbs, Christian, additional, Pfützner, Wolfgang, additional, Radauer, Christian, additional, Bohle, Barbara, additional, Galvao, Clovis Eduardo, additional, Santos-Pinto, Jose Roberto Aparecido, additional, Schwager, Christian, additional, Kull, Skadi, additional, Schocker, Frauke, additional, Behrends, Jochen, additional, Becker, Wolf-Meinhard, additional, Jappe, Uta, additional, Mastrorilli, Carla, additional, Tripodi, Salvatore, additional, Caffarelli, Carlo, additional, Asero, Riccardo, additional, Dondi, Arianna, additional, Ricci, Giampaolo, additional, Dascola, Carlotta Povesi, additional, Calamelli, Elisabetta, additional, Di Rienzo Businco, Andrea, additional, Bianchi, Annamaria, additional, Frediani, Tullio, additional, Verga, Carmen, additional, Iacono, Iride Dello, additional, Peroni, Diego, additional, Pingitore, Giuseppe, additional, Bernardini, Roberto, additional, Matricardi, Paolo Maria, additional, Hofer, Heidi, additional, Asam, Claudia, additional, Hauser, Michael, additional, Himly, Martin, additional, Ebner, Christof, additional, Lemoine, Pierrick, additional, Jain, Karine, additional, Abiteboul, Kathy, additional, Arvidsson, Monica, additional, Rak, Sabina, additional, Mota, Inês, additional, Garcia, Filipe Benito, additional, Gaspar, Angela, additional, Arêde, Cristina, additional, Piedade, Susana, additional, Sampaio, Graça, additional, Pires, Graça, additional, Borrego, Luís Miguel, additional, Santa-Marta, Cristina, additional, Morais-Almeida, Mário, additional, Popescu, Florin-Dan, additional, Vieru, Mariana, additional, Secureanu, Florin-Adrian, additional, Fernandes, Rosa Anita Rodrigues, additional, Carrapatoso, Isabel, additional, Gomes, Raquel, additional, Pereira, Celso, additional, Todo-Bom, Ana, additional, De Basoa, María Cecilia Martín Fernández, additional, Regio, Javier Barrios, additional, De Castro Cordova, Juan, additional, Ferreiro, Antón Fernández, additional, Tsilochristou, Olympia, additional, Perna, Serena, additional, Schwarz, Alina, additional, Rohrbach, Alexander, additional, Cappella, Antonio, additional, Hatzler, Laura, additional, Bauer, Carl-Peter, additional, Hoffmann, Ute, additional, Forster, Johannes, additional, Zepp, Fred, additional, Schuster, Antje, additional, D’amelio, Raffael, additional, Wahn, Ulrich, additional, Keil, Thomas, additional, Lau, Susanne, additional, Apoil, Pol André, additional, Mailhol, Claire, additional, Broué-Chabbert, Anne, additional, Juchet, Agnès, additional, Didier, Alain, additional, Carrer, Elodie, additional, Lanot, Thomas, additional, Blancher, Antoine, additional, Kurtaj, Almedina, additional, Hillebrand, Christoph, additional, Fichtinger, Gerda, additional, Danzer, Martin, additional, Gabriel, Christian, additional, Thalhamer, Theresa, additional, Scheiblhofer, Sandra, additional, Thalhamer, Josef, additional, Weiss, Richard, additional, Wolf, Martin, additional, Pichler, Ulrike, additional, Twaroch, Teresa, additional, Yokoi, Hidenori, additional, Takai, Toshiro, additional, Didierlaurent, Alain, additional, Mari, Adriano, additional, Behrendt, Heidrun, additional, Neubauer, Angela, additional, Stolz, Frank, additional, Ferreira, Fátima, additional, Wallner, Michael, additional, Carvalho, Sara, additional, Lourenço, Tatiana, additional, Cosme, Joana, additional, Duarte, Fátima Cabral, additional, Santos, Amélia Spínola, additional, Costa, Ana Célia, additional, Barbosa, Manuel Pereira, additional, Klinglmayr, Eva, additional, Schweidler, Bettina, additional, Lueftenegger, Lisa, additional, Moser, Stephanie, additional, Doppler, Patrick, additional, Oostingh, Gertie J., additional, Bathke, Arne, additional, Zumbach, Joerg, additional, Panzner, Petr, additional, Vachova, Martina, additional, Vlas, Tomas, additional, Maly, Marek, additional, Posa, Daniela, additional, Hofmaier, Stephanie, additional, Stock, Philippe, additional, Grabenhenrich, Linus, additional, Chen, Kuan-Wei, additional, Resch, Yvonne, additional, Vrtala, Susanne, additional, Valenta, Rudolf, additional, Abramidze, Tamar, additional, Lomidze, Nino, additional, Gotua, Maia, additional, Dapkeviciute, Austeja, additional, Einikyte, Ruta, additional, Norkuniene, Jolita, additional, Skrickiene, Laima, additional, Miskiniene, Asta, additional, Kvedariene, Violeta, additional, Schiener, Maximilian, additional, Moreno-Aguilar, Carmen, additional, Pietsch, Gunilla, additional, Intyre, Mareike Mc, additional, Schwarze, Lea, additional, Rußkamp, Dennis, additional, Spillner, Edzard, additional, Darsow, Ulf, additional, Schmidt-Weber, Carsten, additional, Blank, Simon, additional, Longé, Cyril, additional, Brazdova, Andrea, additional, Brunet, Jean-Louis, additional, Schwartz, Claire, additional, Girodet, Bruno, additional, Lavaud, François, additional, Birnbaum, Joelle, additional, Thi, Nhân Pham, additional, Duchateau, Magalie, additional, Chamot-Rooke, Julia, additional, Guilloux, Laurence, additional, Selva, Marie-Ange, additional, Couderc, Rémy, additional, Sénéchal, Hélène, additional, Sutra, Jean-Pierre, additional, Poncet, Pascal, additional, Augustin, Steffen, additional, Pump, Linda, additional, Wald, Martin, additional, Eichhorn, Thomas, additional, Fischer, Frank, additional, Willers, Christoph, additional, Miehe, Michaela, additional, Plum, Melanie, additional, Wolf, Sara, additional, Jabs, Frederic, additional, Raiber, Tim, additional, Bantleon, Frank, additional, Seismann, Henning, additional, Jakob, Thilo, additional, Apostolovic, Danijela, additional, Tran, Anh Thu, additional, Sanchez-Vidaurre, Sara, additional, Velickovic, Tanja Cirkovic, additional, Starkhammar, Maria, additional, Hamsten, Carl, additional, Van Hage, Marianne, additional, Dubiela, Pawel, additional, Humeniuk, Piotr, additional, Pfeifer, Sabine, additional, Bublin, Merima, additional, Borowski, Tomasz, additional, Hoffmann-Sommergruber, Karin, additional, Verschuren, Martie C. M., additional, Bastiaan-Net, Shanna, additional, Depoortere, Defien, additional, Foetisch, Kay, additional, Scheurer, Stephan, additional, Wichers, Harry J, additional, Noij, Theo, additional, Van Uden, Nikki M.E., additional, Vandenberghe, Karel, additional, Wichers, Harry J., additional, Noij, Theo H. M., additional, Roulias, Anargyros, additional, Parigiani, Maria Alejandra, additional, Ahammer, Linda, additional, Grutsch, Sarina, additional, Tollinger, Martin, additional, Moya, Raquel, additional, López-Matas, Mª Angeles, additional, Reyes, Raquel, additional, Carnés, Jerónimo, additional, Larré, Colette, additional, Rogniaux, Hélène, additional, Lupi, Roberta, additional, Denery-Papini, Sandra, additional, Pablos, Isabel Maria, additional, Eichhorn, Stephanie, additional, Machado, Yoan, additional, Park, Jung-Won, additional, Arora, Naveen, additional, Vieths, Stefan, additional, Tanaka, Charlene, additional, Pineau, Florence, additional, Drouet, Martine, additional, Beaudouin, Etienne, additional, Altenbach, Susan, additional, Mameri, Hamza, additional, Brossard, Chantal, additional, Gaudin, Jean Charles, additional, Moneret-Vautrin, Denise Anne, additional, Paty, Evelyne, additional, Tranquet, Olivier, additional, Masci, Stefania, additional, Moneret-Vautrin, Denise-Anne, additional, Petersen, Arnd, additional, Böttger, Marisa, additional, Rennert, Sandra, additional, Krause, Susanne, additional, Ernst, Martin, additional, Gutsmann, Thomas, additional, Bauer, Johann, additional, Lindner, Buko, additional, Koppelman, Stef, additional, Jayasena, Shyamali, additional, Luykx, Dion, additional, Schepens, Erik, additional, De Jong, Govardus, additional, Isleib, Tom, additional, Nordlee, Julie, additional, Baumert, Joe, additional, Taylor, Steve, additional, Maleki, Soheila, additional, Palladino, Chiara, additional, Sirvent, Sofía, additional, Angelina, Alba, additional, Eiwegger, Thomas, additional, Palomares, Oscar, additional, Breiteneder, Heimo, additional, Claude, Mathilde, additional, Bouchaud, Grégory, additional, Bodinier, Marie, additional, Korte, Robin, additional, Bräcker, Julia, additional, Brockmeyer, Jens, additional, Satoh, Rie, additional, Teshima, Reiko, additional, Tscheppe, Angelika, additional, Palmberger, Dieter, additional, Grabherr, Reingard, additional, Raith, Marianne, additional, Sonnleitner, Linda, additional, Zach, Doris, additional, Woroszylo, Konrad, additional, Focke-Tejkl, Margit, additional, Wank, Herbert, additional, Graf, Thorsten, additional, Kuehn, Annette, additional, Swoboda, Ines, additional, Huber, Sara, additional, Gay-Crosier, Fabienne, additional, Polak, Dominika, additional, Nagl, Birgit, additional, Kitzmüller, Claudia, additional, Samadi, Nazanin, additional, Geyeregger, Rene, additional, Jahn-Schmid, Beatrice, additional, Gomez, Ariel, additional, Haka, Jaana, additional, Hattara, Liisa, additional, Heikkinen, Marika, additional, Niemi, Merja H, additional, Rouvinen, Juha, additional, Saviranta, Petri, additional, Mattila, Pekka, additional, Takkinen, Kristiina, additional, Laukkanen, Marja-Leena, additional, Pablos, Isabel, additional, Kastner, Bianca, additional, Silar, Mira, additional, Selb, Julij, additional, Kogovsek, Rok, additional, Kosnik, Mitja, additional, Korosec, Peter, additional, Pestana, Leticia, additional, Melo, Alcinda Campos, additional, Mendes, Ana, additional, Pedro, Maria Elisa, additional, Santos, Maria Conceição Pereira, additional, Bienvenu, Françoise, additional, Goursaud, Claire, additional, Garnier, Lorna, additional, Jacquenet, Sandrine, additional, Degaud, Michaël, additional, Viel, Sébastien, additional, Barre, Annick, additional, Rougé, Pierre, additional, Bienvenu, Jacques, additional, Vitte, Joana, additional, Bensalah, Amel, additional, Cleach, Isabelle, additional, Mousseau, Laurent, additional, Agabriel, Chantal, additional, Liabeuf, Valérie, additional, Birnbaum, Joëlle, additional, Mège, Jean-Louis, additional, Gardner, James, additional, Gandhi, Minal, additional, Kariyawasam, Harsha, additional, Rotiroti, Giuseppina, additional, Regateiro, Frederico, additional, Faria, Emília, additional, Schmid, Johannes Martin, additional, Dahl, Ronald, additional, Hoffmann, Hans Juergen, additional, Pestana, Letícia, additional, Silva, Diana, additional, Vieira, Teresa, additional, Pereira, Ana Maria, additional, Moreira, André, additional, Delgado, Luís, additional, Prates, Sara, additional, Alves, Cátia, additional, Finelli, Elena, additional, Pinto, Paula Leiria, additional, Cardoso, Bárbara Kong, additional, Cruz, Cíntia, additional, Semedo, Filipa, additional, Tomaz, Elza, additional, Inácio, Filipe, additional, Maity, Santanu, additional, Baricevic-Jones, Ivona, additional, Marsh, Justin T., additional, Johnson, Phil E., additional, Balasundaram, Anuradha, additional, Hope, Anya-May, additional, Taekema, Aafke, additional, Simpson, Angela, additional, Semic-Jusufagic, Aida, additional, Clare Mills, E. N., additional, Nelly, Gourdon Dubois, additional, Laetitia, Sellam, additional, Bruno, Pereira, additional, Elodie, Michaud, additional, Khaled, Messaoudi, additional, Bertrand, Evrard, additional, Jean-Luc, Fauquert, additional, Goodman, Richard E., additional, Plata, Elena Rodríguez, additional, Amaral, Luis, additional, Bartolomé, Borja, additional, Coimbra, Alice, additional, Placido, Jose L, additional, Ganea, Carmen Saviana, additional, Costello, Carol Ann, additional, Sorensen, Martin, additional, Mills, Clare, additional, Rogers, Adrian, additional, Otherhals, Aage, additional, Kalic, Tanja, additional, Ellinger, Isabella, additional, Waltl, Eva, additional, Niederberger-Leppin, Verena, additional, Szczepankiewicz, Dawid, additional, Pruszynska-Oszmalek, Ewa, additional, Skrzypski, Marek, additional, Nowak, Krzysztof W., additional, Szczepankiewicz, Aleksandra, additional, Jang, Gwang-Cheon, additional, Markovic, Iva, additional, Borowski, Andreas, additional, Vetter, Tina, additional, Wohlmann, Andreas, additional, Kuepper, Michael, additional, Friedrich, Karlheinz, additional, Gracia, Ibon Eguiluz, additional, Bosco, Anthony, additional, Dollner, Ralph, additional, Melum, Guro Reinholt, additional, Jones, Anya C, additional, Lexberg, Maria, additional, Holt, Patrick G, additional, Bækkevold, Espen Sønderaal, additional, Jahnsen, Frode Lars, additional, Sobkowiak, Paulina, additional, Rachel, Marta, additional, Narozna, Beata, additional, Jenerowicz, Dorota, additional, Swiatowy, Witold, additional, Breborowicz, Anna, additional, Nestelbacher, Reinhard, additional, and Fukui, Hiroyuki, additional

Published
2016
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16. Chimeras of Bet v 1 and Api g 1 reveal heterogeneous IgE responses in patients with birch pollen allergy

Author

Gepp, Barbara, Lengger, Nina, Bublin, Merima, Hemmer, Wolfgang, Breiteneder, Heimo, and Radauer, Christian

Subjects
Api g 1.0101, IgE epitope mapping, Immunology, Immunology and Allergy, chemical and pharmacologic phenomena, hemic and immune systems, Bet v 1.0101, chimera-based technology, patient-specific IgE repertoire, birch pollen allergy
Abstract

BackgroundCharacterization of IgE-binding epitopes of allergens and determination of their patient-specific relevance is crucial for the diagnosis and treatment of allergy.ObjectiveWe sought to assess the contribution of specific surface areas of the major birch pollen allergen Bet v 1.0101 to binding IgE of individual patients.MethodsFour distinct areas of Bet v 1 representing in total 81% of its surface were grafted onto the scaffold of its homolog, Api g 1.0101, to yield the chimeras Api-Bet-1 to Api-Bet-4. The chimeras were expressed in Escherichia coli and purified. IgE binding of 64 sera from Bet v 1–sensitized subjects with birch pollen allergy was determined by using direct ELISA. Specificity was assessed by means of inhibition ELISA.ResultsrApi g 1.0101, Api-Bet-1, Api-Bet-2, Api-Bet-3, and Api-Bet-4 bound IgE from 44%, 89%, 80%, 78%, and 48% of the patients, respectively. By comparing the amount of IgE binding to the chimeras and to rApi g 1.0101, 81%, 70%, 75%, and 45% of the patients showed significantly enhanced IgE binding to Api-Bet-1, Api-Bet-2, Api-Bet-3, and Api-Bet-4, respectively. The minority (8%) of the sera revealed enhanced IgE binding exclusively to a single chimera, whereas 31% showed increased IgE binding to all 4 chimeras compared with rApi g 1.0101. The chimeras inhibited up to 70% of IgE binding to rBet v 1.0101, confirming the specific IgE recognition of the grafted regions.ConclusionThe Bet v 1–specific IgE response is polyclonal, and epitopes are spread across the entire Bet v 1 surface. Furthermore, the IgE recognition profile of Bet v 1 is highly patient specific.

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18. Identification of probable pectinesterase as a major allergen of pollen of the Asian white birch (Betula platyphylla) in northern China.

Author

Xu Y, Gepp B, Lengger N, Yin J, and Breiteneder H

Abstract

Background: Pollen of the Asian white birch (Betula platyphylla) is a major source of allergens in spring in northern China, yet little research on its pollen allergens has been done so far., Objective: To analyze the B. platyphylla pollen allergen profile of patients from northern China and to identify the major pollen allergens in this patient cohort., Methods: Sera from 35 Chinese patients with birch pollinosis were collected for this study. The IgE-binding proteins in B. platyphylla pollen extract were analyzed by IgE immunoblots. A novel major allergen was purified by cation exchange chromatography and affinity chromatography. Its IgE reactivity was evaluated by ELISA. The protein was excised from a 2D electrophoresis gel and subjected to ESI-QUAD-TOF., Results: In our study cohort, the prevalence of IgE specific for Bet v 1 was 68.8% (n = 22/32) as measured by ImmunoCAP. In immunoblots, two major bands at around 17 kDa and 70 kDa were detected by IgE from 68.5% (24/35) and 65.7% (23/35) of sera, respectively. The 17 kDa band was identified as Bet v 1 by a monoclonal antibody to Bet v 1 and inhibition experiments with recombinant Bet v 1. The 70 kDa band was a polymorphic glycoprotein and 7 isoforms were found. The protein was identified as probably pectinesterase with a molecular weight of 66 kDa and a PI of 5.7., Conclusions: A 66 kDa protein, probably belonging to pectinesterase family, was identified as a novel major allergen of B. platyphylla pollen in patients from northern China.

Published
2023
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