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21 results on '"Lengert, André van Helvoort"'

3. Analysis of the Mutational Landscape of Osteosarcomas Identifies Genes Related to Metastasis and Prognosis and Disrupted Biological Pathways of Immune Response and Bone Development

Author

Pires, Sara Ferreira, primary, Barros, Juliana Sobral de, additional, Costa, Silvia Souza da, additional, Carmo, Gabriel Bandeira do, additional, Scliar, Marília de Oliveira, additional, Lengert, André van Helvoort, additional, Boldrini, Érica, additional, Silva, Sandra Regini Morini da, additional, Vidal, Daniel Onofre, additional, Maschietto, Mariana, additional, and Krepischi, Ana Cristina Victorino, additional

Published
2023
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4. DNA methylation patterns suggest the involvement of DNMT3B and TET1 in osteosarcoma development

Author

Pires, Sara Ferreira, primary, Barros, Juliana Sobral de, additional, Costa, Silvia Souza da, additional, Scliar, Marília de Oliveira, additional, Lengert, André van Helvoort, additional, Boldrini, Erica, additional, Silva, Sandra Regina Morini da, additional, Tasic, Ljubica, additional, Vidal, Daniel Onofre, additional, Krepischi, Ana, additional, and Maschietto, Mariana, additional

Published
2023
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5. Effects of genetic polymorphisms on antioxidant status and concentrations of the metals in the blood of riverside Amazonian communities co-exposed to Hg and Pb

Author

Barcelos, Gustavo Rafael Mazzaron, Souza, Marilesia Ferreira de, Oliveira, Andréia Ávila Soares de, Lengert, André van Helvoort, Oliveira, Marcelo Tempesta de, Camargo, Rossana Batista de Oliveira Godoy, Grotto, Denise, Valentini, Juliana, Garcia, Solange Cristina, Braga, Gilberto Úbida Leite, Cólus, Ilce Mara de Syllos, Adeyemi, Joseph, and Barbosa, Fernando, Jr.

Published
2015
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6. Potential New Therapeutic Approaches for Cisplatin-Resistant Testicular Germ Cell Tumors

Author

Lengert, André van Helvoort, primary, Pereira, Leticia do Nascimento Braga, primary, Cabral, Eduardo Ramos Martins, primary, Gomes, Izabela Natalia Faria, primary, Jesus, Lais Machado de, primary, Gonçalves, Maria Fernanda Santiago, primary, Rocha, Aline Oliveira da, primary, Tassinari, Tiago Alexandre, primary, Silva, Luciane Sussuchi da, primary, Laus, Ana Carolina, primary, Vidal, Daniel Onofre, primary, Pinto, Mariana Tomazini, primary, Reis, Rui Manuel, primary, and Lopes, Luiz Fernando, primary

Published
2022
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8. Tumores de células germinativas não-seminomatosos: insights da transição epitelial-mesenquimal

Author

Ramos, Lorrayne Pereira, primary, Rosa, Marcela Nunez, additional, Lengert, André Van Helvoort, additional, Cabral, Eduardo Ramos Martins, additional, Gomes, Izabela Natalia Faria, additional, Galvão, Janaina Mello Soares, additional, Evangelista, Adriane Feijó, additional, Lopes, Luiz Fernando, additional, Reis, Rui Manuel Vieira, additional, and Pinto, Mariana Tomazini Pinto, additional

Published
2022
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9. Efficacy of Combined Use of Everolimus and Second-Generation Pan-EGRF Inhibitors in KRAS Mutant Non-Small Cell Lung Cancer Cell Lines

Author

da Silva-Oliveira, Renato José, primary, Gomes, Izabela Natalia Faria, additional, da Silva, Luciane Sussuchi, additional, Lengert, André van Helvoort, additional, Laus, Ana Carolina, additional, Melendez, Matias Eliseo, additional, Munari, Carla Carolina, additional, Cury, Fernanda de Paula, additional, Longato, Giovanna Barbarini, additional, and Reis, Rui Manuel, additional

Published
2022
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10. Polymorphisms in glutathione-related genes modify mercury concentrations and antioxidant status in subjects environmentally exposed to methylmercury

Author

Barcelos, Gustavo Rafael Mazzaron, Grotto, Denise, de Marco, Kátia Cristina, Valentini, Juliana, Lengert, André van Helvoort, Oliveira, Andréia Ávila Soares de, Garcia, Solange Cristina, Braga, Gilberto Úbida Leite, Schläwicke Engström, Karin, Cólus, Ilce Mara de Syllos, Broberg, Karin, and Barbosa, Fernando, Jr.

Published
2013
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11. The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death

Author

Ribeiro, Diego Luis, primary, Tuttis, Katiuska, additional, Oliveira, Larissa Cristina Bastos de, additional, Serpeloni, Juliana Mara, additional, Gomes, Izabela Natalia Faria, additional, Lengert, André van Helvoort, additional, Rocha, Cláudia Quintino da, additional, Reis, Rui Manuel, additional, Cólus, Ilce Mara de Syllos, additional, and Antunes, Lusânia Maria Greggi, additional

Published
2022
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12. Detection of Alterations in the Gut Microbiota and Intestinal Permeability in Patients With Hashimoto Thyroiditis

Author

Cayres, Leonardo César de Freitas, primary, de Salis, Larissa Vedovato Vilela, additional, Rodrigues, Guilherme Siqueira Pardo, additional, Lengert, André van Helvoort, additional, Biondi, Ana Paula Custódio, additional, Sargentini, Larissa Donadel Barreto, additional, Brisotti, João Luiz, additional, Gomes, Eleni, additional, and de Oliveira, Gislane Lelis Vilela, additional

Published
2021
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13. Abstract B41: Genomic landscape of somatic mutations in osteosarcomas

Author

Pires, Sara F., primary, Costa, Silvia S., additional, Vidal, Daniel O., additional, Lengert, André van Helvoort, additional, Boldrini, Érica, additional, da Silva, Sandra R.M., additional, Rosenberg, Carla, additional, Lopes, Luiz F., additional, Maschietto, Mariana, additional, and Krepischi, Ana, additional

Published
2020
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14. Characterization of the in vitro cytotoxic effects of brachydins isolated from Fridericia platyphylla in a prostate cancer cell line

Author

Nunes, Higor Lopes, primary, Tuttis, Katiuska, additional, Serpeloni, Juliana Mara, additional, Nascimento, Jessyane Rodrigues do, additional, da Rocha, Claudia Quintino, additional, Silva, Viviane Aline Oliveira, additional, Lengert, André van Helvoort, additional, Reis, Rui Manuel, additional, and de Syllos Cólus, Ilce Mara, additional

Published
2020
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15. The Brazilian TP53 mutation (R337H) and sarcomas

Author

Volc, Sahlua Miguel, primary, Ramos, Cíntia Regina Niederauer, additional, Galvão, Henrique de Campos Reis, additional, Felicio, Paula Silva, additional, Coelho, Aline Silva, additional, Berardineli, Gustavo Noriz, additional, Campacci, Natalia, additional, Sabato, Cristina da Silva, additional, Abrahao-Machado, Lucas Faria, additional, Santana, Iara Viana Vidigal, additional, Campanella, Nathalia, additional, Lengert, André van Helvoort, additional, Vidal, Daniel Onofre, additional, Reis, Rui Manuel, additional, Dantas, Caio F., additional, Coelho, Robson C., additional, Boldrini, Erica, additional, Serrano, Sergio Vicente, additional, and Palmero, Edenir Inêz, additional

Published
2020
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16. Screening and characterization of BRCA2 c.156_157insAlu in Brazil: Results from 1380 individuals from the South and Southeast

Author

Felicio, Paula Silva, Alemar, Barbara, Coelho, Aline Silva, Berardinelli, Gustavo Noriz, Melendez, Matias Eliseo, Lengert, André Van Helvoort, Miche lli, Rodrigo Depieri, Reis, Rui M., Fernandes, Gabriela Carvalho, Ewald, Ingrid Petroni, Bittar, Camila Matzenbacher, Netto, Cristina Brinckmann Oliveira, Artigalas, Osvaldo, Peixoto, Ana, Pinheiro, Manuela, Teixeira, Manuel R., Vargas, Fernando Regla, dos Santos, Anna Cláudia Evangelista, Moreira, Miguel Angelo Martins, Ashton-Prolla, Patricia, and Palmero, Edenir Inêz

Published
2018
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17. Characterization of the invitro cytotoxic effects of brachydins isolated from Fridericia platyphylla in a prostate cancer cell line.

Author

Nunes, Higor Lopes, Tuttis, Katiuska, Serpeloni, Juliana Mara, Nascimento, Jessyane Rodrigues do, da Rocha, Claudia Quintino, Silva, Viviane Aline Oliveira, Lengert, André van Helvoort, Reis, Rui Manuel, and de Syllos Cólus, Ilce Mara

Subjects
DIMETHYL sulfoxide, CRYOPROTECTIVE agents, ETHYLENEDIAMINETETRAACETIC acid, CELL lines, NUCLEAR magnetic resonance, CANCER cells, MICROSATELLITE repeats, CELL cycle
Abstract

Brachydins (Br) A, B, and C are flavonoids extracted from Fridericia platyphylla (Cham.) L.G. Lohmann roots (synonym Arrabidaea brachypoda), whose extract previously exhibited cytotoxic and antitumor activity. In vitro cell culture of human prostate tumor cell line (PC-3) was used to determine cell viability as evidenced by MTT, neutral red, and LDH release using nine concentrations (0.24 to 30.72 µM) of each brachydin. A triple-fluorescent staining assay assessed the mechanism resulting in cell death. Genomic instability and protein expression were evaluated using comet assay and western blot analysis, respectively. The pro-oxidant status was analyzed using the5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) probe. The IC50 values for brachydins BrA, BrB, and BrC were 23.41, 4.28, and 4.44 µM, respectively, and all compounds induced apoptosis and necrosis. BrB and BrC increased p21 levels indicating a possible G1 cell cycle arrest. BrA (6 µM) and BrB (3.84 µM) decreased phospho-AKT (AKT serine/threonine kinase) expression, which also influenced cell cycle and proliferation. BrA, BrB, and BrC elevated cleaved PARP (poly (ADP-ribose) polymerase), a protein related to DNA repair and induction of apoptotic processes. Therefore, this study determined the IC50 values of brachydins in the PC-3 cell line as well as the influence on cell proliferation and cell death processes, such as apoptosis and necrosis, indicating the proteins involved in these processes. ANOVA: Analysis of Variance; BrA: Brachydin A; BrB: Brachydin B; BrC: Brachydin C; CGEN: Genetic Heritage Management Council; CID: Compound identification number; CM-H2DCFDA, 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate, acetyl ester; CO2: Carbon dioxide; DMSO: Dimethyl sulfoxide; DNA: Deoxyribonucleic acid; DTT: Dithiothreitol; DXR: Doxorubicin; ECL: Chemiluminescence; EDTA: Ethylenediaminetetraacetic acid; FBS: Fetal bovine serum; H2O2: Hydrogen peroxide; HRMS: High-Resolution Mass Spectrometry; IC50: Half maximal inhibitory concentration; LDH: Lactate dehydrogenase; MTT, 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide; Na3VO4: Sodium Orthovanadate; NaOH: Sodium hydroxide; NCBI: National Center for Biotechnology Information; NMR: Nuclear Magnetic Resonance; PBS: Phosphate buffer saline; PCR: Polymerase chain reaction; PSMF: Phenylmethylsulfonyl fluoride; RPMI: Roswell Park Memorial Institute Medium; SDS-PAGE: Sodium Dodecyl Sulfate-Polyacrylamide gel electrophoresis; STR: Short tandem repeat; TBS-T: Tris-buffered saline and Polysorbate 20; UPHLC: Ultra-Performance Liquid Chromatography [ABSTRACT FROM AUTHOR]

Published
2020
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19. High Expression of HULC Is Associated with Poor Prognosis in Osteosarcoma Patients

Author

Uzan, Vanessa Regina Maciel, primary, Lengert, André van Helvoort, additional, Boldrini, Érica, additional, Penna, Valter, additional, Scapulatempo-Neto, Cristovam, additional, Scrideli, Carlos Alberto, additional, Filho, Alberto Paiva de Moraes, additional, Cavalcante, Carlos Eduardo Bezerra, additional, de Oliveira, Cleyton Zanardo, additional, Lopes, Luiz Fernando, additional, and Vidal, Daniel Onofre, additional

Published
2016
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20. Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population

Author

de Oliveira, Andréia Ávila Soares, primary, Souza, Marilesia Ferreira de, additional, Lengert, André van Helvoort, additional, de Oliveira, Marcelo Tempesta, additional, Camargo, Rossana Batista de Oliveira Godoy, additional, Braga, Gilberto Úbida Leite, additional, Cólus, Ilce Mara de Syllos, additional, Barbosa, Fernando, additional, and Barcelos, Gustavo Rafael Mazzaron, additional

Published
2014
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21. Cisplatin‑resistant germ cell tumor models: An exploration of the epithelial‑mesenchymal transition regulator SLUG .

Author

Cardoso IIV, Rosa MN, Moreno DA, Tufi LMB, Ramos LP, Pereira LAB, Silva L, Galvão JMS, Tosi IC, Lengert AVH, Da Cruz MC, Teixeira SA, Reis RM, Lopes LF, and Pinto MT

Subjects
Adult, Animals, Humans, Male, Mice, Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use, Cell Line, Tumor, Disease Models, Animal, Gene Expression Regulation, Neoplastic drug effects, Xenograft Model Antitumor Assays, Cisplatin pharmacology, Cisplatin therapeutic use, Drug Resistance, Neoplasm, Epithelial-Mesenchymal Transition drug effects, Neoplasms, Germ Cell and Embryonal metabolism, Neoplasms, Germ Cell and Embryonal pathology, Neoplasms, Germ Cell and Embryonal genetics, Neoplasms, Germ Cell and Embryonal drug therapy, Snail Family Transcription Factors metabolism, Snail Family Transcription Factors genetics, Testicular Neoplasms metabolism, Testicular Neoplasms pathology, Testicular Neoplasms genetics, Testicular Neoplasms drug therapy
Abstract

Germ cell tumors (GCTs) constitute diverse neoplasms arising in the gonads or extragonadal locations. Testicular GCTs (TGCTs) are the predominant solid tumors in adolescents and young men. Despite cisplatin serving as the primary therapeutic intervention for TGCTs, 10‑20% of patients with advanced disease demonstrate resistance to cisplatin‑based chemotherapy, and epithelial‑mesenchymal transition (EMT) is a potential contributor to this resistance. EMT is regulated by various factors, including the snail family transcriptional repressor 2 ( SLUG ) transcriptional factor, and, to the best of our knowledge, remains unexplored within TGCTs. Therefore, the present study investigated the EMT transcription factor SLUG in TGCTs. In silico analyses were performed to investigate the expression of EMT markers in TGCTs. In addition, a cisplatin‑resistant model for TGCTs was developed using the NTERA‑2 cell line, and a mouse model was also established. Subsequently, EMT was assessed both in vitro and in vivo within the cisplatin‑resistant models using quantitative PCR and western blot analyses. The results of the in silico analysis showed that the different histologies exhibited distinct expression profiles for EMT markers. Seminomas exhibited a lower expression of EMT markers, whereas embryonal carcinomas and mixed GCT demonstrated high expression. Notably, patients with lower SLUG expression had longer median progression‑free survival (46.4 months vs. 28.0 months, P=0.022). In the in vitro analysis, EMT‑associated genes [fibronectin; vimentin ( VIM ); actin, α2, smooth muscle; collagen type I α1; transforming growth factor‑β1; and SLUG ] were upregulated in the cisplatin‑resistant NTERA‑2 (NTERA‑2R) cell line after 72 h of cisplatin treatment. Consistent with this finding, the NTERA‑2R mouse model demonstrated a significant upregulation in the expression levels of VIM and SLUG. In conclusion, the present findings suggested that SLUG may serve a crucial role in connecting EMT with the development of cisplatin resistance, and targeting SLUG may be a putative therapeutic strategy to mitigate cisplatin resistance.

Published
2024
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