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13 results on '"Lembo-Fazio, Luigi"'

4. Role of a fluid-phase PRR in fighting an intracellular pathogen: PTX3 in Shigella infection

Author

Ciancarella, Valeria, primary, Lembo-Fazio, Luigi, additional, Paciello, Ida, additional, Bruno, Anna-Karin, additional, Jaillon, Sébastien, additional, Berardi, Sara, additional, Barbagallo, Marialuisa, additional, Meron-Sudai, Shiri, additional, Cohen, Dani, additional, Molinaro, Antonio, additional, Rossi, Giacomo, additional, Garlanda, Cecilia, additional, and Bernardini, Maria Lina, additional

Published
2018
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5. Bradyrhizobium Lipid A: Immunological Properties and Molecular Basis of Its Binding to the Myeloid Differentiation Protein-2/Toll-Like Receptor 4 Complex

Author

Lembo-Fazio, Luigi, primary, Billod, Jean-Marc, additional, Di Lorenzo, Flaviana, additional, Paciello, Ida, additional, Pallach, Mateusz, additional, Vaz-Francisco, Sara, additional, Holgado, Aurora, additional, Beyaert, Rudi, additional, Fresno, Manuel, additional, Shimoyama, Atsushi, additional, Lanzetta, Rosa, additional, Fukase, Koichi, additional, Gully, Djamel, additional, Giraud, Eric, additional, Martín-Santamaría, Sonsoles, additional, Bernardini, Maria-Lina, additional, and Silipo, Alba, additional

Published
2018
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12. Bradyrhizobium Lipid A: Immunological Properties and Molecular Basis of Its Binding to the Myeloid Differentiation Protein-2/Toll-Like Receptor 4 Complex

Author

Luigi Lembo-Fazio, Jean-Marc Billod, Flaviana Di Lorenzo, Ida Paciello, Mateusz Pallach, Sara Vaz-Francisco, Aurora Holgado, Rudi Beyaert, Manuel Fresno, Atsushi Shimoyama, Rosa Lanzetta, Koichi Fukase, Djamel Gully, Eric Giraud, Sonsoles Martín-Santamaría, Maria-Lina Bernardini, Alba Silipo, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), Centro de Investigaciones Biológicas (CIB), Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant’Angelo, University of Naples Federico II = Università degli studi di Napoli Federico II, Diomune SL, Partenaires INRAE, Flanders Institute for Biotechnology, Department of Biomedical Molecular Biology, Universiteit Gent = Ghent University (UGENT), Osaka University, Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Spanish MINECO [CTQ2014-57141-R, CTQ2017-88353-R], European Project: 642157,H2020,H2020-MSCA-ITN-2014,TOLLerant(2015), Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Università degli studi di Napoli Federico II, Universiteit Gent = Ghent University [Belgium] (UGENT), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Lembo-Fazio, Luigi, Billod, Jean-Marc, Di Lorenzo, Flaviana, Paciello, Ida, Pallach, Mateusz, Vaz-Francisco, Sara, Holgado, Aurora, Beyaert, Rudi, Fresno, Manuel, Shimoyama, Atsushi, Lanzetta, Rosa, Fukase, Koichi, Gully, Djamel, Giraud, Eric, Martín-Santamaría, Sonsole, Bernardini, Maria-Lina, and Silipo, Alba

Subjects
0301 basic medicine, INTERFERON-BETA, Lipopolysaccharide, AIRWAY INFLAMMATION, inflammatory cytokines, Biodiversité et Ecologie, myeloid differentiation protein-2/toll-like receptor 4, Lipid A, ACTIVATION, chemistry.chemical_compound, Medicine and Health Sciences, Immunology and Allergy, Bradyrhizobium lipid A, Inflammatory cytokines, Innate immunity, Molecular modeling, Myeloid differentiation protein-2/toll-like receptor 4, Immunology, Receptor, innate immunity, Original Research, Toll-like receptor, PROPOSED STRUCTURE, biology, lipopolysaccharide, Biochemistry, differentiation protein-2toll-like receptor 4, lipids (amino acids, peptides, and proteins), myeloid, réponse immunitaire innée, lcsh:Immunologic diseases. Allergy, LPS, Inflammatory cytokine, 030106 microbiology, BIOLOGICAL-ACTIVITIES, Bradyrhizobium, Biodiversity and Ecology, 03 medical and health sciences, Immune system, propriété immunologique, molecular modeling, differentiation protein-2/toll-like receptor 4, modélisation, ANTAGONIST, Innate immune system, bradyrhizobium, lps, RECOGNITION, Biology and Life Sciences, biology.organism_classification, 030104 developmental biology, chemistry, TLR4, FORCE-FIELD, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, lcsh:RC581-607
Abstract

International audience; Lipopolysaccharides (LPS) are potent activator of the innate immune response through the binding to the myeloid differentiation protein-2 (MD-2)/toll-like receptor 4 (TLR4) receptor complexes. Although a variety of LPSs have been characterized so far, a detailed molecular description of the structure-activity relationship of the lipid A part has yet to be clarified. Photosynthetic Bradyrhizobium strains, symbiont of Aeschynomene legumes, express distinctive LPSs bearing very long-chain fatty acids with a hopanoid moiety covalently linked to the lipid A region. Here, we investigated the immunological properties of LPSs isolated from Bradyrhizobium strains on both murine and human immune systems. We found that they exhibit a weak agonistic activity and, more interestingly, a potent inhibitory effect on MD-2/TLR4 activation exerted by toxic enterobacterial LPSs. By applying computational modeling techniques, we also furnished a plausible explanation for the Bradyrhizobium LPS inhibitory activity at atomic level, revealing that its uncommon lipid A chemical features could impair the proper formation of the receptorial complex, and/or has a destabilizing effect on the pre-assembled complex itself.

Published
2018
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13. Chemistry and biology of the potent endotoxin from a Burkholderia dolosa clinical isolate from a cystic fibrosis patient.

Author

Lorenzo FD, Sturiale L, Palmigiano A, Lembo-Fazio L, Paciello I, Coutinho CP, Sá-Correia I, Bernardini M, Lanzetta R, Garozzo D, Silipo A, and Molinaro A

Subjects
Animals, Burkholderia cepacia complex isolation & purification, Cytokines metabolism, Endotoxins isolation & purification, Endotoxins pharmacology, Female, HEK293 Cells, Humans, Lipopolysaccharide Receptors genetics, Lipopolysaccharide Receptors metabolism, Lipopolysaccharides chemistry, Lipopolysaccharides isolation & purification, Lipopolysaccharides pharmacology, Lymphocyte Antigen 96 genetics, Lymphocyte Antigen 96 metabolism, Macrophages cytology, Macrophages drug effects, Macrophages metabolism, Mice, Mice, Inbred C57BL, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Toll-Like Receptor 4 genetics, Toll-Like Receptor 4 metabolism, Transfection, Burkholderia cepacia complex metabolism, Cystic Fibrosis microbiology, Endotoxins chemistry
Abstract

This is the first report of the chemical and biological properties of the lipooligosaccharide (LOS) endotoxin isolated from Burkholderia dolosa IST4208, an isolate recovered from a cystic fibrosis (CF) patient in a Portuguese CF center. B. dolosa is a member of the Burkholderia cepacia complex, a group of closely related species that are highly problematic and opportunistic pathogens in CF. B. dolosa infection leads to accelerated loss of lung function and decreased survival. The structural determination of its endotoxin was achieved using a combination of chemistry and spectroscopy, and has revealed a novel endotoxin structure. The purified LOS was tested for its immunostimulatory activity on human HEK 293 cells expressing TLR-4, MD-2, and CD-14. In these assays, the LOS showed strong proinflammatory activity., (Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2013
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