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131 results on '"Lekcharoensuk, Chalermpol"'

7. In Vitro Anti-Inflammatory and Regenerative Effects of Autologous Conditioned Serum from Dogs with Osteoarthritis

Author

Soontararak, Sirikul, primary, Ardaum, Piyathida, additional, Senarat, Napaporn, additional, Yangtara, Sarawut, additional, Lekcharoensuk, Chalermpol, additional, Putchong, Iyarath, additional, Kashemsant, Narudee, additional, Vijarnsorn, Monchanok, additional, Chow, Lyndah, additional, Dow, Steven, additional, and Lekcharoensuk, Porntippa, additional

Published
2022
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16. Genetic characterization of porcine circovirus type 2 in piglets from PMWS-affected and -negative farms in Thailand

Author

Lekcharoensuk Chalermpol, Urairong Kitcha, Poolperm Pariwat, Boonsoongnern Alongkot, Jantafong Tippawan, and Lekcharoensuk Porntippa

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract Porcine circovirus type 2 (PCV2) is the major swine pathogen associated with Porcine circovirus associated disease (PCVAD) including post-weaning multisystemic wasting syndrome (PMWS). Currently, there are 4 subtypes of PCV2 (PCV2a, b, c and d) and some epidemiological evidences demonstrated that virulence of PCV2 may relate to its subtypes. Recently, PMWS was observed more frequently in swine farms in Thailand; however, the information regarding to PCV2 subtype involved was limited. Therefore, this study was aimed to determine the association between occurrence of PMWS and PCV2 subtypes as well as genetically characterize PCV2 in Thailand. PCV2 DNA was isolated from faecal swabs and whole blood of piglets from PMWS-affected and -negative farms. The full length ORF2 sequences were compared using multiple alignment. The results showed that PCV2 DNA was detected more frequently in PMWS-affected farms. The nucleotide identities of the ORF2 from 9 PCV2 isolates representing each PMWS-affected farm and one from the negative farm ranged from 92.4 to 99.5% suggesting that there is some genetic variation of PCV2 in Thai swine. The 10 PCV2 isolates were classified into 2 clusters, in which the 7 isolates from PMWS-positive farms were in PCV2b cluster 1 A/B. The remaining isolates were separated in the new subtype called PCV2e. The results suggest the presence of new PCV2 subtypes in addition to PCV2a and PCV2b in Asian swine population. However, correlation between subtypes and virulence of PCV2 infection is not conclusive due to limited number of the PCV2 sequences from PMWS negative farms.

Published
2011
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47. Epidemiology of Canine Calcium Oxalate Uroliths

Author

Lulich, Jody P., primary, Osborne, Carl A., additional, Thumchai, Rosama, additional, Lekcharoensuk, Chalermpol, additional, Ulrich, Lisa K., additional, Koehler, Lori A., additional, Bird, Kathleen A., additional, Swanson, Laura L., additional, and Nakagawa, Yasushi, additional

Published
1999
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49. Development of an inactivated 3Cpro-3ABC (mu3ABC) ELISA to differentiate cattle infected with foot and mouth disease virus from vaccinated cattle

Author

Srisombundit, Vasinee, Tungthumniyom, Nattarat, Linchongsubongkoch, Wilai, Lekcharoensuk, Chalermpol, Sariya, Ladawan, Ramasoota, Pongrama, and Lekcharoensuk, Porntippa

Subjects
*ENZYME-linked immunosorbent assay, *FOOT & mouth disease, *CATTLE vaccination, *COMMUNICABLE diseases, *ENDEMIC animals, *IMMUNE response, *GENE expression
Abstract

Abstract: Foot and mouth disease, a highly contagious disease of cloven-hoofed animals, is still endemic in Asia, Africa, and a few countries in South America. Subclinical and persistent infections usually occur in vaccinated cattle exposed to FMDV. Successful control and eradication measures need a diagnostic assay that can distinguish between immune responses to infection and vaccination. The non-structural 3ABC ELISA is the most reliable differential diagnostic assay. However, expression of the native 3ABC gene in insect cells yielded truncated versions of the proteins; thus, a monoclonal antibody to capture digested proteins is needed to develop the assay. The purpose of this study was to develop a simple indirect 3ABC ELISA using complete 3ABC protein. The full-length mutated 3ABC protein with inactive 3Cpro (mu3ABC) gene was constructed. The histidine-tagged mu3ABC protein was produced in insect cells for easy purification and measuring. This permits simple assay design and reproducible assay development. mu3ABC ELISA had diagnostic specificity and sensitivity of 96.6% and 84%, respectively, compared to Ceditest® FMDV-NS. Agreement of both assays was excellent with κ value of 0.823 (p <0.05). The mu3ABC ELISA could distinguish infected from vaccinated animals. These factors are necessary for the successful development of an in-house NSP-based ELISA. Availability of a reliable assay with acceptable costs would facilitate successful disease control and the establishment of disease-free zones. Expansion of such zones may ultimately decrease the risk of introducing FMDV into disease-free countries, thus accelerating global FMD control. [Copyright &y& Elsevier]

Published
2013
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50. Screening for lead compounds and herbal extracts with potential anti-influenza viral activity.

Author

Klaywong K, Khutrakul G, Choowongkomon K, Lekcharoensuk C, Petcharat N, Leckcharoensuk P, and Ramasoota P

Subjects
Polymerase Chain Reaction, Antiviral Agents pharmacology, Estradiol pharmacology, Influenza A Virus, H5N1 Subtype drug effects, Influenza A Virus, H5N1 Subtype metabolism, Lead pharmacology, Plant Extracts pharmacology, RNA, Double-Stranded metabolism, RNA, Viral metabolism, Veratridine pharmacology, Viral Nonstructural Proteins metabolism
Abstract

Nonstructural protein 1 (NS1) of the highly pathogenic avian influenza virus (H5N1) contains a conserved RNA binding domain (RBD) that inhibits antiviral functions of host-innate immune response. Dimerization of NS1 forms a central groove and binds to double stranded (ds) RNA. This region might serve as a potential drug target. In this study, three dimensional structure model of NS1 RBD protein was constructed and virtual screening was performed to identify lead compounds that bound within and around the central groove. The virtual screening showed that 5 compounds bound within the central groove with binding energy ranging between -16.05 and -17.36 Kcal/mol. Two commercially available compounds, estradiol and veratridine, were selected for using in an in vitro screening assay. The results showed that neither of the compounds could inhibit the association between dsRNA and NS1 RBD protein. In addition, 34 herbal extracts were examined for their inhibitory effects. Five of them were able to inhibit association between NS1 RBD and dsRNA in electrophoresis mobility shift assay. Four herbs, Terminalia belirica, Salacia chinensis, Zingiber montanum and Peltophorum pterocarpum, could reduce > 50% of infectivity of H5N1 in a cell-based assay, and it is worth further studying their potential use as source of antiviral drugs.

Published
2014

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