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9. About the mechanism of interference of silver staining with peptide mass spectrometry

Author

Richert, Sophie, Luche, Sylvie, Chevallet, Mireille, Van Dorsselaer, Alain, Leize-Wagner, Emmanuelle, and Rabilloud, Thierry

Subjects
Quantitative Biology - Genomics
Abstract

The mechanism by which silver staining of proteins in polyacrylamide gels interferes with mass spectrometry of peptides produced by proteolysis has been investigated. It was demonstrated that this interference increases with time between silver staining and gel processing, although the silver image is constant. This suggested an important role of the formaldehyde used in silver staining development in this interference process. Consequently, a formaldehyde-free staining protocol has been devised, using carbohydrazide as the developing agent. This protocol showed much increased peptide coverage and retained the sensitivity of silver staining. These results were however obtained at the expense of an increased background in the stained gels and of a reduced staining homogeneity.

Published
2012
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10. Power and limitations of electrophoretic separations in proteomics strategies

Author

Rabilloud, Thierry, Vaezzadeh, Ali R, Potier, Noelle, Lelong, Cécile, Leize-Wagner, Emmanuelle, and Chevallet, Mireille

Subjects
Quantitative Biology - Genomics
Abstract

Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry. These techniques, dealing with proteins or peptides, can rely on chromatography or electrophoresis. In this review, the electrophoretic techniques are under scrutiny. Their principles are recalled, and their applications for peptide and protein separations are presented and critically discussed. In addition, the features that are specific to gel electrophoresis and that interplay with mass spectrometry (i.e., protein detection after electrophoresis, and the process leading from a gel piece to a solution of peptides) are also discussed.

Published
2009
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11. Organoplatinum‐Bridged Cyclotribenzylene Dimers

Author

Zhang, Jing, primary, Aribot, Frédéric, additional, Chambron, Jean‐Claude, additional, Zorn, Nathalie, additional, Leize‐Wagner, Emmanuelle, additional, Jean, Marion, additional, Vanthuyne, Nicolas, additional, Espinosa, Enrique, additional, and Aubert, Emmanuel, additional

Published
2023
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12. Oxidative stress response: a proteomic view

Author

Rabilloud, Thierry, Chevallet, Mireille, Luche, Sylvie, and Leize-Wagner, Emmanuelle

Subjects
Quantitative Biology - Genomics
Abstract

The oxidative stress response is characterized by various effects on a range of biologic molecules. When examined at the protein level, both expression levels and protein modifications are altered by oxidative stress. While these effects have been studied in the past by classic biochemical methods, the recent onset of proteomics methods has allowed the oxidative stress response to be studied on a much wider scale. The input of proteomics in the study of oxidative stress response and in the evidence of an oxidative stress component in biologic phenomena is reviewed in this paper.

Published
2008
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14. Improved mass spectrometry compatibility is afforded by ammoniacal silver staining

Author

Chevallet, Mireille, Diemer, Hélène, Luche, Sylvie, van Dorsselaer, Alain, Rabilloud, Thierry, and Leize-Wagner, Emmanuelle

Subjects
Quantitative Biology - Genomics
Abstract

Sequence coverage in MS analysis of protein digestion-derived peptides is a key issue for detailed characterization of proteins or identification at low quantities. In gel-based proteomics studies, the sequence coverage greatly depends on the protein detection method. It is shown here that ammoniacal silver detection methods offer improved sequence coverage over standard silver nitrate methods, while keeping the high sensitivity of silver staining. With the development of 2D-PAGE-based proteomics, another burden is placed on the detection methods used for protein detection on 2-D-gels. Besides the classical requirements of linearity, sensitivity, and homogeneity from one protein to another, detection methods must now take into account another aspect, namely their compatibility with MS. This compatibility is evidenced by two different and complementary aspects, which are (i) the absence of adducts and artefactual modifications on the peptides obtained after protease digestion of a protein detected and digested in - gel, and (ii) the quantitative yield of peptides recovered after digestion and analyzed by the mass spectrometer. While this quantitative yield is not very important per se, it is however a crucial parameter as it strongly influences the S/N of the mass spectrum and thus the number of peptides that can be detected from a given protein input, especially at low protein amounts. This influences in turn the sequence coverage and thus the detail of the analysis provided by the mass spectrometer., Comment: website publisher http://www.interscience.wiley.com

Published
2006
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15. Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view

Author

Chevallet, Mireille, Lescuyer, Pierre, Diemer, Hélène, van Dorsselaer, Alain, Leize-Wagner, Emmanuelle, and Rabilloud, Thierry

Subjects
Quantitative Biology - Genomics
Abstract

The proper functioning of mitochondria requires that both the mitochondrial and the nuclear genome are functional. To investigate the importance of the mitochondrial genome, which encodes only 13 subunits of the respiratory complexes, the mitochondrial rRNAs and a few tRNAs, we performed a comparative study on the 143B cell line and on its Rho-0 counterpart, i.e., devoid of mitochondrial DNA. Quantitative differences were found, of course in the respiratory complexes subunits, but also in the mitochondrial translation apparatus, mainly mitochondrial ribosomal proteins, and in the ion and protein import system, i.e., including membrane proteins. Various mitochondrial metabolic processes were also altered, especially electron transfer proteins and some dehydrogenases, but quite often on a few proteins for each pathway. This study also showed variations in some hypothetical or poorly characterized proteins, suggesting a mitochondrial localization for these proteins. Examples include a stomatin-like protein and a protein sharing homologies with bacterial proteins implicated in tyrosine catabolism. Proteins involved in apoptosis control are also found modulated in Rho-0 mitochondria., Comment: website publisher: http://www3.interscience.wiley.com/

Published
2006
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16. About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis

Author

Luche, Sylvie, Diemer, Hélène, Tastet, Chistophe, Chevallet, Mireille, Van Dorsselaer, Alain, Leize-Wagner, Emmanuelle, and Rabilloud, Thierry

Subjects
Quantitative Biology - Genomics
Abstract

The influence of thiol blocking on the resolution of basic proteins by two-dimensional electrophoresis was investigated. Cysteine blocking greatly increased resolution and decreased streaking, especially in the basic region of the gels. Two strategies for cysteine blocking were found to be efficient: classical alkylation with maleimide derivatives and mixed disulfide exchange with an excess of a low molecular weight disulfide. The effect on resolution was significant enough to allow correct resolution of basic proteins with in-gel rehydration on wide gradients (e.g. 3-10 and 4-12), but anodic cup-loading was still required for basic gradients (e.g. 6-12 or 8-12). These results demonstrate that thiol-related problems are not solely responsible for streaking of basic proteins on two-dimensional gels., Comment: web publisher http://www.interscience.wiley.com

Published
2006
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20. Nucleos’ID: A New Search Engine Enabling the Untargeted Identification of RNA Post-transcriptional Modifications from Tandem Mass Spectrometry Analyses of Nucleosides

Author

Gosset-Erard, Clarisse, primary, Didierjean, Mévie, additional, Pansanel, Jérome, additional, Lechner, Antony, additional, Wolff, Philippe, additional, Kuhn, Lauriane, additional, Aubriet, Frédéric, additional, Leize-Wagner, Emmanuelle, additional, Chaimbault, Patrick, additional, and François, Yannis-Nicolas, additional

Published
2023
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24. Contributors

Author

Beck, Alain, primary, Breadmore, Michael C., additional, Bressan, Lucas P., additional, Chagas, Cyro L.S., additional, Coltro, Wendell K.T., additional, d’Orlyé, Fanny, additional, da Silva, José A.F., additional, de Jesus, Dosil P., additional, de Jong, Gerhardus, additional, Desiderio, Claudia, additional, François, Yannis-Nicolas, additional, Gahoual, Rabah, additional, García-Campaña, Ana M., additional, Giorgetti, Jérémie, additional, Grochocki, Wojciech, additional, Hage, David S., additional, Hernández-Mesa, Maykel, additional, Hirokawa, Takeshi, additional, Höcker, Oliver, additional, Jones, Laurel, additional, Jooß, Kevin, additional, Kašička, Václav, additional, Kenndler, Ernst, additional, Lara, Francisco J., additional, Leize-Wagner, Emmanuelle, additional, Li, Shao-Ping, additional, Maier, Norbert M., additional, Marina, María Luisa, additional, Markuszewski, Michal J., additional, Martelli, Claudia, additional, Mikšík, Ivan, additional, Moreira, Roger C., additional, Moreno-González, David, additional, Neusüß, Christian, additional, Pyell, Ute, additional, Quirino, Joselito P., additional, Ramírez-García, Gonzalo, additional, Römer, Jennifer, additional, Sánchez-López, Elena, additional, Sänger-van de Griend, Cari E., additional, Shimura, Kiyohito, additional, Sola, Laura, additional, Štěpánová, Sille, additional, Thormann, Wolfgang, additional, Trapiella-Alfonso, Laura, additional, Van Schepdael, Ann, additional, Varenne, Anne, additional, Wang, Yan, additional, Xue, Yun, additional, Yan, Chao, additional, Yang, Feng-Qing, additional, Zhang, Chenhua, additional, Zhang, Qian, additional, and Zhao, Jing, additional

Published
2018
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27. Comprehensive multilevel characterization of biologics using sheathless capillary electrophoresis hyphenated to tandem mass spectrometry

Author

Gahoual, Rabah, Biacchi, Michael, Busnel, Jean-Marc, Beck, Alain, Francois, Yannis-Nicolas, and Leize-Wagner, Emmanuelle

Subjects
Biological products -- Research -- Properties, Mass spectrometry -- Usage -- Methods, Electrophoresis -- Research, Chemistry
Abstract

An innovative coupling of capillary electrophoresis and tandem mass spectrometry (CE-MS-MS) provides excellent results for the characterization of the primary structure of biologics. Using a single 200-fmol injection, it is [...]

Published
2015

31. Additional file 5 of Insights into metazoan evolution from alvinella pompejana cDNAs

Author

Gagnière, Nicolas, Jollivet, Didier, Boutet, Isabelle, Brélivet, Yann, Busso, Didier, Da Silva, Corinne, Gaill, Françoise, Higuet, Dominique, Hourdez, Stéphane, Knoops, Bernard, Lallier, François, Leize-Wagner, Emmanuelle, Mary, Jean, Moras, Dino, Perrodou, Emmanuel, Jean-François Rees, Segurens, Béatrice, Shillito, Bruce, Tanguy, Arnaud, Thierry, Jean-Claude, Weissenbach, Jean, Wincker, Patrick, Zal, Franck, Poch, Olivier, and Lecompte, Odile

Abstract

Additional file 5: Figure S5. Amino acid composition across model taxa. (PDF 40 KB)

Published
2021
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32. Additional file 2 of Insights into metazoan evolution from alvinella pompejana cDNAs

Author

Gagnière, Nicolas, Jollivet, Didier, Boutet, Isabelle, Brélivet, Yann, Busso, Didier, Da Silva, Corinne, Gaill, Françoise, Higuet, Dominique, Hourdez, Stéphane, Knoops, Bernard, Lallier, François, Leize-Wagner, Emmanuelle, Mary, Jean, Moras, Dino, Perrodou, Emmanuel, Jean-François Rees, Segurens, Béatrice, Shillito, Bruce, Tanguy, Arnaud, Thierry, Jean-Claude, Weissenbach, Jean, Wincker, Patrick, Zal, Franck, Poch, Olivier, and Lecompte, Odile

Abstract

Additional file 2: Figure S2. Distribution of complete protein lengths. (PDF 92 KB)

Published
2021
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33. Additional file 10 of Insights into metazoan evolution from alvinella pompejana cDNAs

Author

Gagnière, Nicolas, Jollivet, Didier, Boutet, Isabelle, Brélivet, Yann, Busso, Didier, Da Silva, Corinne, Gaill, Françoise, Higuet, Dominique, Hourdez, Stéphane, Knoops, Bernard, Lallier, François, Leize-Wagner, Emmanuelle, Mary, Jean, Moras, Dino, Perrodou, Emmanuel, Jean-François Rees, Segurens, Béatrice, Shillito, Bruce, Tanguy, Arnaud, Thierry, Jean-Claude, Weissenbach, Jean, Wincker, Patrick, Zal, Franck, Poch, Olivier, and Lecompte, Odile

Subjects
Data_FILES
Abstract

Authors’ original file for figure 5

Published
2021
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34. Additional file 4 of Insights into metazoan evolution from alvinella pompejana cDNAs

Author

Gagnière, Nicolas, Jollivet, Didier, Boutet, Isabelle, Brélivet, Yann, Busso, Didier, Da Silva, Corinne, Gaill, Françoise, Higuet, Dominique, Hourdez, Stéphane, Knoops, Bernard, Lallier, François, Leize-Wagner, Emmanuelle, Mary, Jean, Moras, Dino, Perrodou, Emmanuel, Jean-François Rees, Segurens, Béatrice, Shillito, Bruce, Tanguy, Arnaud, Thierry, Jean-Claude, Weissenbach, Jean, Wincker, Patrick, Zal, Franck, Poch, Olivier, and Lecompte, Odile

Abstract

Additional file 4: Figure S4. Example of Alvinella proteins mapped on a KEGG pathway. (PDF 82 KB)

Published
2021
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35. Additional file of Insights into metazoan evolution from alvinella pompejana cDNAs

Author

Gagnière, Nicolas, Jollivet, Didier, Boutet, Isabelle, Brélivet, Yann, Busso, Didier, Da Silva, Corinne, Gaill, Françoise, Higuet, Dominique, Hourdez, Stéphane, Knoops, Bernard, Lallier, François, Leize-Wagner, Emmanuelle, Mary, Jean, Moras, Dino, Perrodou, Emmanuel, Jean-François Rees, Segurens, Béatrice, Shillito, Bruce, Tanguy, Arnaud, Thierry, Jean-Claude, Weissenbach, Jean, Wincker, Patrick, Zal, Franck, Poch, Olivier, and Lecompte, Odile

Abstract

Additional file of Insights into metazoan evolution from alvinella pompejana cDNAs

Published
2021
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36. Additional file 6 of Insights into metazoan evolution from alvinella pompejana cDNAs

Author

Gagnière, Nicolas, Jollivet, Didier, Boutet, Isabelle, Brélivet, Yann, Busso, Didier, Da Silva, Corinne, Gaill, Françoise, Higuet, Dominique, Hourdez, Stéphane, Knoops, Bernard, Lallier, François, Leize-Wagner, Emmanuelle, Mary, Jean, Moras, Dino, Perrodou, Emmanuel, Jean-François Rees, Segurens, Béatrice, Shillito, Bruce, Tanguy, Arnaud, Thierry, Jean-Claude, Weissenbach, Jean, Wincker, Patrick, Zal, Franck, Poch, Olivier, and Lecompte, Odile

Subjects
Data_FILES
Abstract

Authors’ original file for figure 1

Published
2021
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37. Additional file 9 of Insights into metazoan evolution from alvinella pompejana cDNAs

Author

Gagnière, Nicolas, Jollivet, Didier, Boutet, Isabelle, Brélivet, Yann, Busso, Didier, Da Silva, Corinne, Gaill, Françoise, Higuet, Dominique, Hourdez, Stéphane, Knoops, Bernard, Lallier, François, Leize-Wagner, Emmanuelle, Mary, Jean, Moras, Dino, Perrodou, Emmanuel, Jean-François Rees, Segurens, Béatrice, Shillito, Bruce, Tanguy, Arnaud, Thierry, Jean-Claude, Weissenbach, Jean, Wincker, Patrick, Zal, Franck, Poch, Olivier, and Lecompte, Odile

Subjects
Data_FILES
Abstract

Authors’ original file for figure 4

Published
2021
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38. Additional file 7 of Insights into metazoan evolution from alvinella pompejana cDNAs

Author

Gagnière, Nicolas, Jollivet, Didier, Boutet, Isabelle, Brélivet, Yann, Busso, Didier, Da Silva, Corinne, Gaill, Françoise, Higuet, Dominique, Hourdez, Stéphane, Knoops, Bernard, Lallier, François, Leize-Wagner, Emmanuelle, Mary, Jean, Moras, Dino, Perrodou, Emmanuel, Jean-François Rees, Segurens, Béatrice, Shillito, Bruce, Tanguy, Arnaud, Thierry, Jean-Claude, Weissenbach, Jean, Wincker, Patrick, Zal, Franck, Poch, Olivier, and Lecompte, Odile

Subjects
Data_FILES
Abstract

Authors’ original file for figure 2

Published
2021
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39. Ruthenium(II) complexes with improved photophysical properties based on planar 4'-(2-pyrimidinyl)-2,2':6',2'-terpyridine ligands

Author

Yuan-Qing Fang, Taylor, Nicholas J., Laverdiere, Francois, Hana, Garry S., Nierengarten, Helene, Leize-Wagner, Emmanuelle, Van Dorssealaer, Alain, Loiseau, Frederique, Nastasi, Francesco, and Campagna, Sebastino

Subjects
Ruthenium -- Optical properties, Ruthenium -- Chemical properties, Ligands -- Optical properties, Ligands -- Chemical properties, Excited state chemistry -- Research, Chemistry
Abstract

The synthesis and characterization of the new family of ligand and their Ru(II) complexes is described and also the effect of 2-pyrimidinyl subunits on the photophysical properties of the [[Ru[(tpy).sub.2]].sup.2} chromophore is investigated. The metal complexes obtained exhibit improved photophysical property due to an enhanced metal-to-ligand charge transfer (MLCT)-metal centered (MC) energy gap and an increased delocalization in the acceptor ligand of the MLCT emitting excited states.

Published
2007

40. NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods

Author

De Leoz, Maria Lorna A., Duewer, David L, Fung, Adam, Liu, Lily, Kei Yau, Hoi, Potter, Oscar, Staples, Gregory O, Furuki, Kenichiro, Frenkel, Ruth, Hu, Yunli, Socic, Zoran, Zhang, Peiqing, Altmann, Friedrich, Grunwald-Grube, Clemens, Shao, Chun, Zaia, Joseph, Evers, Waltraud, Pengelley, Stuart, Detlev, Suckau, Wiechmann, Anja, Resemann, Anja, Jabs, Wolfgang, Beck, Alain, Froehlich, John W, Huang, Chuncui, Yan, Li, Yaming, Liu, Sun, Shiwei, Wang, Yaojun, Seo, Youngsuk, An, Hyun Joo, Reichardt, Niels-Christian, Ruiz, Juan Echevarria, Archer-Hartmann, Stephanie, Azadi, Parastoo, Bell, Len, Lakos, Zsuzsanna, An, Yanming, Cipollo, John F, Pucic-Bakovic, Maja, Stambuk, Jerko, Lauc, Gordan, Li, Xu, Wang, Peng George, Bock, Andreas, Hennig, Rene, Rapp, Erdmann, Creskey, Marybeth, Cyr, Terry D, Nakano, Miyako, Sugiyama, Taiki, Leung, Pui-King Amy, Link-Lenczowski, Pawel, Jaworek, Jolanta, Yoo, Jong Shin, Kim, Sa-Rang, Suh, Soo-Kyung, de Haan, Noortje, Falck, David, Lageveen-Kammeijer, Guinevere S. M., Wuhrer, Manfred, Emery, Robert J, Liew, Li Phing, Royle, Louise, Urbanowicz, Paulina A, Packer, Nicolle H, Song, Xiaomin, Everest-Dass, Arun, Lattova, Erika, Cajic, Samanta, Alagesan, Kathirvel, Kolarich, Daniel, Kasali, Toyin, Lindo, Viv, Chen, Yuetian, Goswami, Kudrat, Gau, Brian, Amunugama, Ravi, Jones, Richard, Stroop, Corné J M, Kato, Koichi, Yagi, Hirokazu, Kondo, Sachiko, Yuen, C T, Harazono, Akira, Shi, Xiafeng, Magnelli, Paula, Kasper, Brian T, Mahai, Lara, Harvey, David J, O'Flaherty, Roisin, Rudd, Pauline M., Saldova, Radka, Hecht, Elizabeth S, Muddiman, David C, Kang, Jichao, Bhoskar, Prachi, Menard, Daniele, Saati, Andrew, Merle, Christine, Mast, Steven, Tep, Sam, Truong, Jennie, Nishikaze, Takashi, Sekiya, Sadanori, Shafer, Aaron, Funaoka, Sohei, Toyoda, Masaaki, de Vreugd, Peter, Caron, Cassie, Pradhan, Pralima, Tan, Niclas Chiang, Mechref, Yehia, Patil, Sachin, Rohrer, Jeffrey S, Chakrabarti, Ranjan, Dadke, Disha, Lahori, Mohammedazam, Zou, Chunxia, Cairo, Christopher, Reiz, Béla, Whittal, Randy M, Lebrilla, Carlito B, Wu, Lauren, Guttman, Andras, Szigeti, Marton, Kremkow, Benjamin G, Lee, Kevin H, Sihlbom, Carina, Adamczyk, Barbara, Jin, Chunsheng, Karlsson, Niclas G, Omros, Jessica, Larson, Goran, Nilsson, Jonas, Meyer, Bernd, Alena, Wiegandt, Komatsu, Emy, Perreault, Helene, Bodnar, Edward D, Said, Nassur, Francois, Yannis-Nicolas, Leize-Wagner, Emmanuelle, Maier, Sandra, Zeck, Anne, Heck, Albert J R, Yang, Yang, Haselberg, Rob, Qing Yu, Ying, Alley, William, Leone, Joseph W, Yuan, Hua, Stein, Stephen E, De Leoz, Maria Lorna A., Duewer, David L, Fung, Adam, Liu, Lily, Kei Yau, Hoi, Potter, Oscar, Staples, Gregory O, Furuki, Kenichiro, Frenkel, Ruth, Hu, Yunli, Socic, Zoran, Zhang, Peiqing, Altmann, Friedrich, Grunwald-Grube, Clemens, Shao, Chun, Zaia, Joseph, Evers, Waltraud, Pengelley, Stuart, Detlev, Suckau, Wiechmann, Anja, Resemann, Anja, Jabs, Wolfgang, Beck, Alain, Froehlich, John W, Huang, Chuncui, Yan, Li, Yaming, Liu, Sun, Shiwei, Wang, Yaojun, Seo, Youngsuk, An, Hyun Joo, Reichardt, Niels-Christian, Ruiz, Juan Echevarria, Archer-Hartmann, Stephanie, Azadi, Parastoo, Bell, Len, Lakos, Zsuzsanna, An, Yanming, Cipollo, John F, Pucic-Bakovic, Maja, Stambuk, Jerko, Lauc, Gordan, Li, Xu, Wang, Peng George, Bock, Andreas, Hennig, Rene, Rapp, Erdmann, Creskey, Marybeth, Cyr, Terry D, Nakano, Miyako, Sugiyama, Taiki, Leung, Pui-King Amy, Link-Lenczowski, Pawel, Jaworek, Jolanta, Yoo, Jong Shin, Kim, Sa-Rang, Suh, Soo-Kyung, de Haan, Noortje, Falck, David, Lageveen-Kammeijer, Guinevere S. M., Wuhrer, Manfred, Emery, Robert J, Liew, Li Phing, Royle, Louise, Urbanowicz, Paulina A, Packer, Nicolle H, Song, Xiaomin, Everest-Dass, Arun, Lattova, Erika, Cajic, Samanta, Alagesan, Kathirvel, Kolarich, Daniel, Kasali, Toyin, Lindo, Viv, Chen, Yuetian, Goswami, Kudrat, Gau, Brian, Amunugama, Ravi, Jones, Richard, Stroop, Corné J M, Kato, Koichi, Yagi, Hirokazu, Kondo, Sachiko, Yuen, C T, Harazono, Akira, Shi, Xiafeng, Magnelli, Paula, Kasper, Brian T, Mahai, Lara, Harvey, David J, O'Flaherty, Roisin, Rudd, Pauline M., Saldova, Radka, Hecht, Elizabeth S, Muddiman, David C, Kang, Jichao, Bhoskar, Prachi, Menard, Daniele, Saati, Andrew, Merle, Christine, Mast, Steven, Tep, Sam, Truong, Jennie, Nishikaze, Takashi, Sekiya, Sadanori, Shafer, Aaron, Funaoka, Sohei, Toyoda, Masaaki, de Vreugd, Peter, Caron, Cassie, Pradhan, Pralima, Tan, Niclas Chiang, Mechref, Yehia, Patil, Sachin, Rohrer, Jeffrey S, Chakrabarti, Ranjan, Dadke, Disha, Lahori, Mohammedazam, Zou, Chunxia, Cairo, Christopher, Reiz, Béla, Whittal, Randy M, Lebrilla, Carlito B, Wu, Lauren, Guttman, Andras, Szigeti, Marton, Kremkow, Benjamin G, Lee, Kevin H, Sihlbom, Carina, Adamczyk, Barbara, Jin, Chunsheng, Karlsson, Niclas G, Omros, Jessica, Larson, Goran, Nilsson, Jonas, Meyer, Bernd, Alena, Wiegandt, Komatsu, Emy, Perreault, Helene, Bodnar, Edward D, Said, Nassur, Francois, Yannis-Nicolas, Leize-Wagner, Emmanuelle, Maier, Sandra, Zeck, Anne, Heck, Albert J R, Yang, Yang, Haselberg, Rob, Qing Yu, Ying, Alley, William, Leone, Joseph W, Yuan, Hua, and Stein, Stephen E

Abstract

Glycosylation is a topic of intense current interest in the development of biopharmaceuticals because it is related to drug safety and efficacy. This work describes results of an interlaboratory study on the glycosylation of the Primary Sample (PS) of NISTmAb, a monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, and hospital sectors in Europe, North America, Asia, and Australia submitted a total of 103 reports on glycan distributions. The principal objective of this study was to report and compare results for the full range of analytical methods presently used in the glycosylation analysis of mAbs. Therefore, participation was unrestricted, with laboratories choosing their own measurement techniques. Protein glycosylation was determined in various ways, including at the level of intact mAb, protein fragments, glycopeptides, or released glycans, using a wide variety of methods for derivatization, separation, identification, and quantification. Consequently, the diversity of results was enormous, with the number of glycan compositions identified by each laboratory ranging from 4 to 48. In total, one hundred sixteen glycan compositions were reported, of which 57 compositions could be assigned consensus abundance values. These consensus medians provide community-derived values for NISTmAb PS. Agreement with the consensus medians did not depend on the specific method or laboratory type. The study provides a view of the current state-of-the-art for biologic glycosylation measurement and suggests a clear need for harmonization of glycosylation analysis methods.

Published
2020

45. Alkynylgold(I) C3‐Chiral Concave Complexes: Aggregation and Luminescence.

Author

Zhang, Jing, Schaly, Astrid, Chambron, Jean‐Claude, Vincent, Bruno, Zorn, Nathalie, Leize‐Wagner, Emmanuelle, Jean, Marion, and Vanthuyne, Nicolas

Subjects
LUMINESCENCE, ENANTIOMERS, MIXTURES, FLUORESCENCE, LIGANDS (Chemistry), SOLVENTS, PHOSPHORESCENCE, PHOSPHORESCENCE spectroscopy
Abstract

Chiral gold(I) acetylide trinuclear complexes 1–3 based on the cyclotribenzylene platform and terminal PR3 ligands (R=Ph, Et, and Cy, respectively), were characterized and their light emission studied. They exhibited long‐lived blue phosphorescence in CHCl3 and a weak fluorescence in the UV. In MeOH/CHCl3 mixtures of >1:1 volume ratio, 1 and 2 exhibited a new emission band at ca. 540 nm that developed at the expense of the UV emission. DLS studies demonstrated the presence of molecular aggregates of Ø 30–80 nm. The green emission observed in MeOH‐rich solvent mixtures was therefore induced by aggregation, and could originate from Au⋅⋅⋅Au interactions. The AIE spectrum of 3 was observed only in solutions containing 99 % of MeOH, and correlated with its solid state emission. The AIE profiles of the enantiomers of 1 differed from that of rac‐1, suggesting that the latter is a true racemate. [ABSTRACT FROM AUTHOR]

Published
2022
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49. NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods

Author

De Leoz, Maria Lorna A., primary, Duewer, David L., additional, Fung, Adam, additional, Liu, Lily, additional, Yau, Hoi Kei, additional, Potter, Oscar, additional, Staples, Gregory O., additional, Furuki, Kenichiro, additional, Frenkel, Ruth, additional, Hu, Yunli, additional, Sosic, Zoran, additional, Zhang, Peiqing, additional, Altmann, Friedrich, additional, Grunwald-Grube, Clemens, additional, Shao, Chun, additional, Zaia, Joseph, additional, Evers, Waltraud, additional, Pengelley, Stuart, additional, Suckau, Detlev, additional, Wiechmann, Anja, additional, Resemann, Anja, additional, Jabs, Wolfgang, additional, Beck, Alain, additional, Froehlich, John W., additional, Huang, Chuncui, additional, Li, Yan, additional, Liu, Yaming, additional, Sun, Shiwei, additional, Wang, Yaojun, additional, Seo, Youngsuk, additional, An, Hyun Joo, additional, Reichardt, Niels-Christian, additional, Ruiz, Juan Echevarria, additional, Archer-Hartmann, Stephanie, additional, Azadi, Parastoo, additional, Bell, Len, additional, Lakos, Zsuzsanna, additional, An, Yanming, additional, Cipollo, John F., additional, Pucic-Bakovic, Maja, additional, Štambuk, Jerko, additional, Lauc, Gordan, additional, Li, Xu, additional, Wang, Peng George, additional, Bock, Andreas, additional, Hennig, René, additional, Rapp, Erdmann, additional, Creskey, Marybeth, additional, Cyr, Terry D., additional, Nakano, Miyako, additional, Sugiyama, Taiki, additional, Leung, Pui-King Amy, additional, Link-Lenczowski, Paweł, additional, Jaworek, Jolanta, additional, Yang, Shuang, additional, Zhang, Hui, additional, Kelly, Tim, additional, Klapoetke, Song, additional, Cao, Rui, additional, Kim, Jin Young, additional, Lee, Hyun Kyoung, additional, Lee, Ju Yeon, additional, Yoo, Jong Shin, additional, Kim, Sa-Rang, additional, Suh, Soo-Kyung, additional, de Haan, Noortje, additional, Falck, David, additional, Lageveen-Kammeijer, Guinevere S.M., additional, Wuhrer, Manfred, additional, Emery, Robert J., additional, Kozak, Radoslaw P., additional, Liew, Li Phing, additional, Royle, Louise, additional, Urbanowicz, Paulina A., additional, Packer, Nicolle H., additional, Song, Xiaomin, additional, Everest-Dass, Arun, additional, Lattová, Erika, additional, Cajic, Samanta, additional, Alagesan, Kathirvel, additional, Kolarich, Daniel, additional, Kasali, Toyin, additional, Lindo, Viv, additional, Chen, Yuetian, additional, Goswami, Kudrat, additional, Gau, Brian, additional, Amunugama, Ravi, additional, Jones, Richard, additional, Stroop, Corné J.M., additional, Kato, Koichi, additional, Yagi, Hirokazu, additional, Kondo, Sachiko, additional, Yuen, C.T., additional, Harazono, Akira, additional, Shi, Xiaofeng, additional, Magnelli, Paula E., additional, Kasper, Brian T., additional, Mahal, Lara, additional, Harvey, David J., additional, O'Flaherty, Roisin, additional, Rudd, Pauline M., additional, Saldova, Radka, additional, Hecht, Elizabeth S., additional, Muddiman, David C., additional, Kang, Jichao, additional, Bhoskar, Prachi, additional, Menard, Daniele, additional, Saati, Andrew, additional, Merle, Christine, additional, Mast, Steven, additional, Tep, Sam, additional, Truong, Jennie, additional, Nishikaze, Takashi, additional, Sekiya, Sadanori, additional, Shafer, Aaron, additional, Funaoka, Sohei, additional, Toyoda, Masaaki, additional, de Vreugd, Peter, additional, Caron, Cassie, additional, Pradhan, Pralima, additional, Tan, Niclas Chiang, additional, Mechref, Yehia, additional, Patil, Sachin, additional, Rohrer, Jeffrey S., additional, Chakrabarti, Ranjan, additional, Dadke, Disha, additional, Lahori, Mohammedazam, additional, Zou, Chunxia, additional, Cairo, Christopher, additional, Reiz, Béla, additional, Whittal, Randy M., additional, Lebrilla, Carlito B., additional, Wu, Lauren, additional, Guttman, Andras, additional, Szigeti, Marton, additional, Kremkow, Benjamin G., additional, Lee, Kelvin H., additional, Sihlbom, Carina, additional, Adamczyk, Barbara, additional, Jin, Chunsheng, additional, Karlsson, Niclas G., additional, Örnros, Jessica, additional, Larson, Göran, additional, Nilsson, Jonas, additional, Meyer, Bernd, additional, Wiegandt, Alena, additional, Komatsu, Emy, additional, Perreault, Helene, additional, Bodnar, Edward D., additional, Said, Nassur, additional, Francois, Yannis-Nicolas, additional, Leize-Wagner, Emmanuelle, additional, Maier, Sandra, additional, Zeck, Anne, additional, Heck, Albert J.R., additional, Yang, Yang, additional, Haselberg, Rob, additional, Yu, Ying Qing, additional, Alley, William, additional, Leone, Joseph W., additional, Yuan, Hua, additional, and Stein, Stephen E., additional

Published
2020
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