91 results on '"Leiming, Xu"'
Search Results
2. Intelligent nanovesicle for remodeling tumor microenvironment and circulating tumor chemoimmunotherapy amplification
- Author
-
Manxiu Huai, Yingjie Wang, Junhao Li, Jiaxing Pan, Fang Sun, Feiyu Zhang, Yi Zhang, and Leiming Xu
- Subjects
Pancreatic ductal adenocarcinoma ,Chemoimmunotherapy amplification ,5-FU ,Nanovesicle ,Kyn-AHR axis ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Imperceptible examination and unideal treatment effect are still intractable difficulties for the clinical treatment of pancreatic ductal adenocarcinoma (PDAC). At present, despite 5-fluorouracil (5-FU), as a clinical first-line FOLFIRINOX chemo-drug, has achieved significant therapeutic effects. Nevertheless, these unavoidable factors such as low solubility, lack of biological specificity and easy to induce immunosuppressive surroundings formation, severely limit their treatment in PDAC. As an important source of energy for many tumor cells, tryptophan (Trp), is easily degraded to kynurenine (Kyn) by indolamine 2,3- dioxygenase 1 (IDO1), which activates the axis of Kyn-AHR to form special suppressive immune microenvironment that promotes tumor growth and metastasis. However, our research findings that 5-FU can induce effectively immunogenic cell death (ICD) to further treat tumor by activating immune systems, while the secretion of interferon-γ (IFN-γ) re-induce the Kyn-AHR axis activation, leading to poor treatment efficiency. Therefore, a metal matrix protease-2 (MMP-2) and endogenous GSH dual-responsive liposomal-based nanovesicle, co-loading with 5-FU (anti-cancer drug) and NLG919 (IDO1 inhibitor), was constructed (named as ENP919@5-FU). The multifunctional ENP919@5-FU can effectively reshape the tumor immunosuppression microenvironment to enhance the effect of chemoimmunotherapy, thereby effectively inhibiting cancer growth. Mechanistically, PDAC with high expression of MMP-2 will propel the as-prepared nanovesicle to dwell in tumor region via shedding PEG on the nanovesicle surface, effectively enhancing tumor uptake. Subsequently, the S-S bond containing nanovesicle was cut via high endogenous GSH, leading to the continued release of 5-FU and NLG919, thereby enabling circulating chemoimmunotherapy to effectively cause tumor ablation. Moreover, the combination of ENP919@5-FU and PD-L1 antibody (αPD-L1) showed a synergistic anti-tumor effect on the PDAC model with abdominal cavity metastasis. Collectively, ENP919@5-FU nanovesicle, as a PDAC treatment strategy, showed excellent antitumor efficacy by remodeling tumor microenvironment to circulate tumor chemoimmunotherapy amplification, which has promising potential in a precision medicine approach.
- Published
- 2024
- Full Text
- View/download PDF
3. Association between pesticide exposure and thyroid function: analysis of Chinese and NHANES databases
- Author
-
Leiming Xu, Shengkai Yang, Longqing Wang, Jinxin Qiu, Hai Meng, Lulu Zhang, Wenwen Sun, and Aifeng He
- Subjects
pesticides ,thyroid function ,Bayesian kernel machine regression ,restricted cubic spline curve ,NHANES database ,Public aspects of medicine ,RA1-1270 - Abstract
BackgroundPesticides are widely used in agricultural activities. Although pesticide use is known to cause damage to the human body, its relationship with thyroid function remains unclear. Therefore, this study aimed to investigate the association between pesticide exposure and thyroid function.MethodsThe Chinese database used included 60 patients with pyrethroid poisoning and 60 participants who underwent health checkups between June 2022 and June 2023. The NHANES database included 1,315 adults enrolled from 2007 to 2012. The assessed pesticide and their metabolites included 2,4-dichlorophenoxyacetic acid (2,4-D), 4-fluoro-3-phenoxybenzoic acid (4F3PB), para-nitrophenol (PN), 3-phenoxybenzoic acid (3P), and trans-dichlorovinyl-dimethylcyclopropane carboxylic acid (TDDC). The evaluated indicators of thyroid function were measured by the blood from the included population. The relationship between pesticide exposure and thyroid function indexes was investigated using linear regression, Bayesian kernel machine regression (BKMR), restricted cubic spline (RCS), and weighted quantile sum (WQS) models.ResultsThe Chinese data showed that pesticide exposure was negatively correlated with the thyroid function indicators FT4, TT4, TgAb, and TPOAb (all p
- Published
- 2024
- Full Text
- View/download PDF
4. FIT-based risk-stratification model effectively screens colorectal neoplasia and early-onset colorectal cancer in Chinese population: a nationwide multicenter prospective study
- Author
-
Shengbing Zhao, Shuling Wang, Peng Pan, Tian Xia, Rundong Wang, Quancai Cai, Xin Chang, Fan Yang, Lun Gu, Zixuan He, Jiayi Wu, Qianqian Meng, Tongchang Wang, Qiwen Fang, Xiaomei Mou, Honggang Yu, Jinghua Zheng, Cheng Bai, Yingbin Zou, Dongfeng Chen, Xiaoping Zou, Xu Ren, Leiming Xu, Ping Yao, Guangsu Xiong, Xu Shu, Tong Dang, Li Zhang, Wen Wang, Shengchao Kang, Hongfei Cao, Aixia Gong, Jun Li, Heng Zhang, Yiqi Du, Zhaoshen Li, Yu Bai, and Gastrointestinal Early Cancer Prevention & Treatment Alliance of China (GECA)
- Subjects
Risk-stratification model ,Fecal immunochemical test ,Early-onset colorectal cancer ,Colorectal cancer screening ,Diseases of the blood and blood-forming organs ,RC633-647.5 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract No fully validated risk-stratification strategies have been established in China where colonoscopies resources are limited. We aimed to develop and validate a fecal immunochemical test (FIT)-based risk-stratification model for colorectal neoplasia (CN); 10,164 individuals were recruited from 175 centers nationwide and were randomly allocated to the derivation (n = 6776) or validation cohort (n = 3388). Multivariate logistic analyses were performed to develop the National Colorectal Polyp Care (NCPC) score, which formed the risk-stratification model along with FIT. The NCPC score was developed from eight independent predicting factors and divided into three levels: low risk (LR 0–14), intermediate risk (IR 15–17), and high risk (HR 18–28). Individuals with IR or HR of NCPC score or FIT+ were classified as increased-risk individuals in the risk-stratification model and were recommended for colonoscopy. The IR/HR of NCPC score showed a higher prevalence of CNs (21.8%/32.8% vs. 11.0%, P
- Published
- 2022
- Full Text
- View/download PDF
5. Effect of a probiotic formula on gastrointestinal health, immune responses and metabolic health in adults with functional constipation or functional diarrhea
- Author
-
Yanyi Zheng, Leiming Xu, Silu Zhang, Yanwen Liu, Jiayi Ni, and Guoxun Xiao
- Subjects
functional constipation ,functional diarrhea ,gastrointestinal health ,immune responses ,metabolic health ,probiotics ,Nutrition. Foods and food supply ,TX341-641 - Abstract
ObjectiveOur aim was to determine the efficacy of four-week probiotic supplementation on gastrointestinal health. The secondary objectives were to assess probiotic effects on immune reaction, as well as weight control and metabolic health.MethodsWe conducted two randomized sub-trials, respectively, among subjects who were diagnosed with functional constipation (FC) or functional diarrhea (FDr) according to the Rome IV criteria. In each sub-trial, 70 eligible Chinese adults were randomized to receive a multi-strain probiotic combination or a placebo. Gastrointestinal symptoms, defecation habits, stool characteristics, blood and fecal biochemistry markers, anthropometrics measures, stress-associated responses, and intestinal flora changes were assessed at baseline and after probiotics intervention.ResultsFour weeks of probiotic supplementation reduced overall gastrointestinal symptoms scores in FC participants (p
- Published
- 2023
- Full Text
- View/download PDF
6. Galanin ameliorates liver inflammation and fibrosis in mice by activating AMPK/ACC signaling and modifying macrophage inflammatory phenotype
- Author
-
Lingnan He, Chao Huang, Hui Wang, Naibin Yang, Jianbin Zhang, Leiming Xu, Ting Gu, Zhenghong Li, and Yuanwen Chen
- Subjects
galanin ,non-alcoholic steatohepatitis ,liver fibrosis ,macrophage ,AMPK ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Background and aimsGalanin is a naturally occurring peptide that plays a critical role in regulating inflammation and energy metabolism, with expression in the liver. The exact involvement of galanin in non-alcoholic fatty liver disease and related fibrosis remains controversial.MethodsThe effects of subcutaneously administered galanin were studied in mice with non-alcoholic steatohepatitis (NASH) induced by a high-fat and high-cholesterol diet for 8 weeks, and in mice with liver fibrosis induced by CCl4 for 7 weeks. The underlying mechanism was also studied in vitro on murine macrophage cells (J774A.1 and RAW264.7).ResultsGalanin reduced inflammation, CD68-positive cell count, MCP-1 level, and mRNA levels of inflammation-related genes in the liver of NASH mice. It also mitigated liver injury and fibrosis caused by CCl4. In vitro, galanin had anti-inflammatory effects on murine macrophages, including reduced phagocytosis and intracellular reactive oxygen species (ROS). Galanin also activated AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) signaling.ConclusionGalanin ameliorates liver inflammation and fibrosis in mice, potentially by modifying macrophage inflammatory phenotype and activating AMPK/ACC signaling.
- Published
- 2023
- Full Text
- View/download PDF
7. An attention base U-net for parotid tumor autosegmentation
- Author
-
Xianwu Xia, Jiazhou Wang, Sheng Liang, Fangfang Ye, Min-Ming Tian, Weigang Hu, and Leiming Xu
- Subjects
parotid ,auto-segmentation ,artificial intelligence (AI) ,neoplasms ,diagnosis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
A parotid neoplasm is an uncommon condition that only accounts for less than 3% of all head and neck cancers, and they make up less than 0.3% of all new cancers diagnosed annually. Due to their nonspecific imaging features and heterogeneous nature, accurate preoperative diagnosis remains a challenge. Automatic parotid tumor segmentation may help physicians evaluate these tumors. Two hundred eighty-five patients diagnosed with benign or malignant parotid tumors were enrolled in this study. Parotid and tumor tissues were segmented by 3 radiologists on T1-weighted (T1w), T2-weighted (T2w) and T1-weighted contrast-enhanced (T1wC) MR images. These images were randomly divided into two datasets, including a training dataset (90%) and an validation dataset (10%). A 10-fold cross-validation was performed to assess the performance. An attention base U-net for parotid tumor autosegmentation was created on the MRI T1w, T2 and T1wC images. The results were evaluated in a separate dataset, and the mean Dice similarity coefficient (DICE) for both parotids was 0.88. The mean DICE for left and right tumors was 0.85 and 0.86, respectively. These results indicate that the performance of this model corresponds with the radiologist’s manual segmentation. In conclusion, an attention base U-net for parotid tumor autosegmentation may assist physicians to evaluate parotid gland tumors.
- Published
- 2022
- Full Text
- View/download PDF
8. Multi-Task Refined Boundary-Supervision U-Net (MRBSU-Net) for Gastrointestinal Stromal Tumor Segmentation in Endoscopic Ultrasound (EUS) Images.
- Author
-
Xinyi Li 0002, Yi Guo 0002, Fei Jiang, Leiming Xu, Feng Shen, Zhendong Jin, and Yuanyuan Wang 0001
- Published
- 2020
- Full Text
- View/download PDF
9. Regorafenib-loaded poly (lactide-co-glycolide) microspheres designed to improve transarterial chemoembolization therapy for hepatocellular carcinoma
- Author
-
Xiang Li, Guangwei He, Feng Su, Zhaoxing Chu, Leiming Xu, Yazhong Zhang, Jianping Zhou, and Yang Ding
- Subjects
Regorafenib ,Microspheres ,Transarterial chemoembolization ,Hepatocellular carcinoma ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Transarterial chemoembolization (TACE) has been widely introduced to treat hepatocellular carcinoma (HCC) especially for unresectable patients for decades. However, TACE evokes an angiogenic response due to the secretion of vascular endothelial growth factor (VEGF), resulting in the formation of new blood vessels and eventually tumor recurrence. Thus, we aimed to develop regorafenib (REGO)-loaded poly (lactide-co-glycolide) (PLGA) microspheres that enabled localized and sustained drug delivery to limit proangiogenic responses following TACE in HCC treatment. REGO-loaded PLGA microspheres were prepared using the emulsion-solvent evaporation/extraction method, in which DMF was selected as an organic phase co-solvent. Accordingly, we optimized the proportion of DMF, which the optimal ratio to DCM was 1:9 (v/v). After preparation, the microspheres provided high drug loading capacity of 28.6%, high loading efficiency of 91.5%, and the average particle size of 149 µm for TACE. IR spectra and XRD were applied to confirming sufficient REGO entrapment. The in vitro release profiles demonstrated sustained drug release of microspheres for more than 30 d To confirm the role of REGO-loaded microspheres in TACE, the cell cytotoxic activity on HepG2 cells and anti-angiogenic effects in HUVECs Tube-formation assay were studied in combination with miriplatin. Moreover, the microspheres indicated the potential of antagonizing miriplatin resistance of HepG2 cells in vitro. Pharmacokinetics preliminary studies exhibited that REGO could be sustainably released from microspheres for more than 30 d after TACE in vivo. In vivo anti-tumor efficacy was further determined in HepG2 xenograft tumor mouse model, demonstrating that REGO microspheres could improve the antitumor efficacy of miriplatin remarkably compared with miriplatin monotherapy. In conclusion, the obtained REGO microspheres demonstrated promising therapeutic effects against HCC when combined with TACE.
- Published
- 2020
- Full Text
- View/download PDF
10. Regulating Glucose Metabolism with Prodrug Nanoparticles for Promoting Photoimmunotherapy of Pancreatic Cancer
- Author
-
Fang Sun, Qiurong Zhu, Tianliang Li, Madiha Saeed, Zhiai Xu, Feisheng Zhong, Rundi Song, Manxiu Huai, Mingyue Zheng, Cen Xie, Leiming Xu, and Haijun Yu
- Subjects
glucose metabolism ,immunogenic cell death ,pancreatic cancer ,photoimmunotherapy ,prodrug nanoparticles ,Science - Abstract
Abstract The low immunogenicity, insufficient infiltration of T lymphocytes, and dismal response to immune checkpoint blockade therapy pose major difficulties in immunotherapy of pancreatic cancer. Photoimmunotherapy by photodynamic therapy (PDT) can induce an antitumor immune response by triggering immunogenic cell death in the tumor cells. Notwithstanding, PDT‐driven oxygen consumption and microvascular damage can further aggravate hypoxia to exaggerates glycolysis, leading to lactate accumulation and immunosuppressive tumor microenvironment. Herein, a supramolecular prodrug nanoplatform codelivering a photosensitizer and a prodrug of bromodomain‐containing protein 4 inhibitor (BRD4i) JQ1 for combinatory photoimmunotherapy of pancreatic cancer are demonstrated. The nanoparticles are fabricated by host–guest complexation between cyclodextrin‐grafted hyaluronic acid (HA‐CD) and adamantine‐conjugated heterodimers of pyropheophorbide a (PPa) and JQ1, respectively. HA can achieve active tumor targeting by recognizing highly expressed CD44 on the surface of pancreatic tumors. PPa‐mediated PDT can enhance the immunogenicity of the tumor cells and promote intratumoral infiltration of the cytotoxic T lymphocytes. Meanwhile, JQ1 combats PDT‐mediated immune evasion through inhibiting expression of c‐Myc and PD‐L1, which are key regulators of tumor glycolysis and immune evasion. Collectively, this study presents a novel strategy to enhance photoimmunotherapy of the pancreatic cancer by provoking T cells activation and overcoming adaptive immune resistance.
- Published
- 2021
- Full Text
- View/download PDF
11. Ultrasonic Image Features under the Intelligent Algorithm in the Diagnosis of Severe Sepsis Complicated with Renal Injury
- Author
-
Leiming Xu, Xin Wang, Pu Pu, Suhui Li, Yongzheng Shao, and Yong Li
- Subjects
Article Subject ,General Immunology and Microbiology ,Applied Mathematics ,General Medicine ,Acute Kidney Injury ,Kidney ,Prognosis ,General Biochemistry, Genetics and Molecular Biology ,Intensive Care Units ,ROC Curve ,Artificial Intelligence ,Sepsis ,Modeling and Simulation ,Humans ,Ultrasonics ,Retrospective Studies - Abstract
This research was aimed at analyzing the diagnosis of severe sepsis complicated with acute kidney injury (AKI) by ultrasonic image information based on the artificial intelligence pulse-coupled neural network (PCNN) algorithm and at improving the diagnostic accuracy and efficiency of clinical severe sepsis complicated with AKI. In this research, 50 patients with sepsis complicated with AKI were collected as the observation group and 50 patients with sepsis as the control group. All patients underwent ultrasound examination. The clinical data of the two groups were collected, and the scores of acute physiology and chronic health assessment (APACHE II) and sequential organ failure assessment (SOFA) were compared. The ultrasonic image information enhancement algorithm based on artificial intelligence PCNN is constructed and simulated and is compared with the maximum between-class variance (OSTU) algorithm and the maximum entropy algorithm. The results showed that the PCNN algorithm was superior to the OSTU algorithm and maximum entropy algorithm in the segmentation results of severe sepsis combined with AKI in terms of regional consistency (UM), regional contrast (CM), and shape measure (SM). The acute physiology and chronic health evaluation (APACHE II) and sequential organ failure assessment (SOFA) scores in the observation group were substantially higher than those in the control group ( P < 0.05 ). The interlobular artery resistance index (RI) in the observation group was substantially higher than that in the control group ( P < 0.05 ). Moreover, the mean transit time (mTT) in the observation group was significantly higher than that in the control group ( 4.85 ± 1.27 vs. 3.42 ± 1.04 ), and the perfusion index (PI) was significantly lower than that in the control group ( 134.46 ± 17.29 vs. 168.37 ± 19.28 ), with statistical significance ( P < 0.05 ). In summary, it can substantially increase ultrasonic image information based on the artificial intelligence PCNN algorithm. The RI, mTT, and PI of the renal interlobular artery level in ultrasound images can be used as indexes for the diagnosis of severe sepsis complicated with AKI.
- Published
- 2022
12. Self-Adaptive Nanoregulator to Mitigate Dynamic Immune Evasion of Pancreatic Cancer.
- Author
-
Jiaxing Pan, Yi Lai, Shunan Zhang, Huijuan Zhang, Yiming Shan, Lujia Huang, Feng Wang, Haijun Yu, Leiming Xu, and Zhiai Xu
- Published
- 2023
- Full Text
- View/download PDF
13. Preparation of Highly Efficient 3d Bioi Microspheres for Visible-Light-Induced Photocatalytic Degradation of Tetracycline
- Author
-
Saifeng Zhang, Huimei Duan, Rui Zhang, Qiuyu Zhu, Chu Wang, Xiao Sun, Yangyang Li, Yuanxu Liu, and Leiming Xu
- Published
- 2023
14. Sequentially Activatable Polypeptide Nanoparticles for Combinatory Photodynamic Chemotherapy of Breast Cancer
- Author
-
Huijuan Zhang, Jiaxing Pan, Tingting Wang, Yi Lai, Xiaoying Liu, Fangmin Chen, Leiming Xu, Xiongwei Qu, Xiuli Hu, and Haijun Yu
- Subjects
Mice ,Drug Delivery Systems ,Photosensitizing Agents ,Paclitaxel ,Photochemotherapy ,Polymers ,Cell Line, Tumor ,Neoplasms ,Animals ,Nanoparticles ,General Materials Science ,Peptides ,Micelles - Abstract
Stimuli-activatable nanomaterials hold significant promise for tumor-specific drug delivery by recognizing the internal or external stimulus. Herein, we reported a dual-responsive and biodegradable polypeptide nanoparticle (PPTP@PTX
- Published
- 2022
15. A Distributed Fast Light-path Set-up Protocol for Grid over OBS Networks.
- Author
-
Min Li, Xueyue Cao, Leiming Xu, Ping Zhang, Chi Yuan, Shuping Peng 0001, and Zhengbin Li
- Published
- 2008
- Full Text
- View/download PDF
16. On the Problem of Channel Assignment for Multi-NIC Multihop Wireless Networks.
- Author
-
Leiming Xu, Yong Xiang, and Meilin Shi
- Published
- 2005
- Full Text
- View/download PDF
17. Depletion of Thymosin β4 Promotes the Proliferation, Migration, and Activation of Human Hepatic Stellate Cells
- Author
-
Yongtao Xiao, Chunying Qu, Wensong Ge, Baocan Wang, Jianxin Wu, Leiming Xu, and Yingwei Chen
- Subjects
Proliferation ,Thymosin β4 ,Liver fibrosis ,Hepatic stellate cell ,Migration ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background & Aims: It has recently been reported that thymosin beta-4 (Tβ4) has anti-fibrogenic effects in human hepatic stellate cells (HSCs) in vitro, but the mechanisms underlying these effects remain unclear. The aim of this study was to investigate the roles of Tβ4 in the proliferation, migration, and activation of HSCs. Methods: Enzyme-linked immunosorbent assays (ELISA), immunohistochemistry, and western blot assays were utilized to determine the expression levels of Tβ4 in serum, liver tissues, and LX-2 cells. Tβ4 was depleted in LX-2 cells using small interfering RNAs (siRNAs). Cell proliferation was analyzed using cell counting kit-8 (CCK-8) viability assays, and cell migration was investigated using wound-healing and transwell migration assays. Results: The expression of Tβ4 was significantly reduced during the progression of liver fibrosis. The depletion of Tβ4 significantly promoted the proliferation and migration of LX-2 cells via the activation of the PI3K/Akt signaling pathway. The pro-migratory and pro-proliferative effects of Tβ4 depletion in LX-2 cells can be counteracted by treatment with the Akt inhibitor MK-2206. In addition, Tβ4 depletion was also associated with the activation of HSCs via the enhanced expression of α-smooth muscle actin (α-SMA) and vimentin. Conclusions: Our results suggest that Tβ4 participates in liver fibrosis by inhibiting the migration, proliferation, and activation of HSCs and that Tβ4 may be an effective target in the treatment of liver fibrosis.
- Published
- 2014
- Full Text
- View/download PDF
18. Facile defect construction of TiO2 nanotube for excellent photocatalytic degradation of tetracycline under visible light
- Author
-
Chu Wang, Yuanxu Liu, Yangyang Li, Xiao Sun, Leiming Xu, and Weixin Huang
- Subjects
General Chemical Engineering ,General Physics and Astronomy ,General Chemistry - Published
- 2023
19. A multi-channel medium access control protocol for multicast in mobile ad-hoc network.
- Author
-
Yao Zhao, Yong Xiang, Leiming Xu, and Meilin Shi
- Published
- 2003
- Full Text
- View/download PDF
20. Ad hoc networking based on TD-SCDMA.
- Author
-
Leiming Xu, Bo Pang, and Yong Xiang
- Published
- 2003
- Full Text
- View/download PDF
21. Gastrointestinal stromal tumors diagnosis on multi-center endoscopic ultrasound images using multi-scale image normalization and transfer learning
- Author
-
Chengcheng Liu, Yi Guo, Fei Jiang, Leiming Xu, Feng Shen, Zhendong Jin, and Yuanyuan Wang
- Subjects
Biomaterials ,Machine Learning ,Gastrointestinal Stromal Tumors ,Biomedical Engineering ,Biophysics ,Humans ,Health Informatics ,Bioengineering ,Diagnosis, Computer-Assisted ,Neural Networks, Computer ,Information Systems - Abstract
BACKGROUND: Automated diagnosis of gastrointestinal stromal tumors’ (GISTs) cancerization is an effective way to improve the clinical diagnostic accuracy and reduce possible risks of biopsy. Although deep convolutional neural networks (DCNNs) have proven to be very effective in many image classification problems, there is still a lack of studies on endoscopic ultrasound (EUS) images of GISTs. It remains a substantial challenge mainly due to the data distribution bias of multi-center images, the significant inter-class similarity and intra-class variation, and the insufficiency of training data. OBJECTIVE: The study aims to classify GISTs into higher-risk and lower-risk categories. METHODS: Firstly, a novel multi-scale image normalization block is designed to perform same-size and same-resolution resizing on the input data in a parallel manner. A dilated mask is used to obtain a more accurate interested region. Then, we construct a multi-way feature extraction and fusion block to extract distinguishable features. A ResNet-50 model built based on transfer learning is utilized as a powerful feature extractor for tumors’ textural features. The tumor size features and the patient demographic features are also extracted respectively. Finally, a robust XGBoost classifier is trained on all features. RESULTS: Experimental results show that our proposed method achieves the AUC score of 0.844, which is superior to the clinical diagnosis performance. CONCLUSIONS: Therefore, the results have provided a solid baseline to encourage further researches in this field.
- Published
- 2022
22. Regorafenib-loaded poly (lactide-co-glycolide) microspheres designed to improve transarterial chemoembolization therapy for hepatocellular carcinoma
- Author
-
Leiming Xu, Jianping Zhou, Guangwei He, Yazhong Zhang, Yang Ding, Feng Su, Xiang Li, and Zhaoxing Chu
- Subjects
Hepatocellular carcinoma ,Pharmaceutical Science ,02 engineering and technology ,Pharmacology ,010402 general chemistry ,01 natural sciences ,Transarterial chemoembolization ,chemistry.chemical_compound ,Pharmacokinetics ,In vivo ,Regorafenib ,medicine ,lcsh:RM1-950 ,021001 nanoscience & nanotechnology ,medicine.disease ,In vitro ,Microspheres ,0104 chemical sciences ,Vascular endothelial growth factor ,Original Research Paper ,PLGA ,lcsh:Therapeutics. Pharmacology ,chemistry ,Drug delivery ,0210 nano-technology - Abstract
Transarterial chemoembolization (TACE) has been widely introduced to treat hepatocellular carcinoma (HCC) especially for unresectable patients for decades. However, TACE evokes an angiogenic response due to the secretion of vascular endothelial growth factor (VEGF), resulting in the formation of new blood vessels and eventually tumor recurrence. Thus, we aimed to develop regorafenib (REGO)-loaded poly (lactide-co-glycolide) (PLGA) microspheres that enabled localized and sustained drug delivery to limit proangiogenic responses following TACE in HCC treatment. REGO-loaded PLGA microspheres were prepared using the emulsion-solvent evaporation/extraction method, in which DMF was selected as an organic phase co-solvent. Accordingly, we optimized the proportion of DMF, which the optimal ratio to DCM was 1:9 (v/v). After preparation, the microspheres provided high drug loading capacity of 28.6%, high loading efficiency of 91.5%, and the average particle size of 149 µm for TACE. IR spectra and XRD were applied to confirming sufficient REGO entrapment. The in vitro release profiles demonstrated sustained drug release of microspheres for more than 30 d To confirm the role of REGO-loaded microspheres in TACE, the cell cytotoxic activity on HepG2 cells and anti-angiogenic effects in HUVECs Tube-formation assay were studied in combination with miriplatin. Moreover, the microspheres indicated the potential of antagonizing miriplatin resistance of HepG2 cells in vitro. Pharmacokinetics preliminary studies exhibited that REGO could be sustainably released from microspheres for more than 30 d after TACE in vivo. In vivo anti-tumor efficacy was further determined in HepG2 xenograft tumor mouse model, demonstrating that REGO microspheres could improve the antitumor efficacy of miriplatin remarkably compared with miriplatin monotherapy. In conclusion, the obtained REGO microspheres demonstrated promising therapeutic effects against HCC when combined with TACE., Graphical abstract REGO-loaded PLGA microspheres demonstrated superior anti-angiogenic effect, potential of antagonizing miriplatin resistance and remarkable antitumor efficacy compared with miriplatin monotherapy.Image, graphical abstract
- Published
- 2020
23. Multi-Task Refined Boundary-Supervision U-Net (MRBSU-Net) for Gastrointestinal Stromal Tumor Segmentation in Endoscopic Ultrasound (EUS) Images
- Author
-
Zhendong Jin, Yuanyuan Wang, Xinyi Li, Leiming Xu, Feng Shen, Yi Guo, and Fei Jiang
- Subjects
Endoscopic ultrasound ,General Computer Science ,Computer science ,Stability (learning theory) ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Boundary (topology) ,02 engineering and technology ,03 medical and health sciences ,boundary-supervision ,multi-task ,0202 electrical engineering, electronic engineering, information engineering ,medicine ,General Materials Science ,Segmentation ,030304 developmental biology ,0303 health sciences ,medicine.diagnostic_test ,business.industry ,General Engineering ,Pattern recognition ,tumor segmentation ,Cross entropy ,endoscopic ultrasound ,Path (graph theory) ,020201 artificial intelligence & image processing ,Artificial intelligence ,Noise (video) ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,Gastrointestinal stromal tumor ,business ,Focus (optics) ,lcsh:TK1-9971 - Abstract
The diagnosis of risk level of gastrointestinal stromal tumor (GIST) is of great clinical significance. The morphology of GIST in endoscopic ultrasound (EUS) images has been normally used by radiologists to diagnosis the risk level of GISTs. Hence, accurate segmentation of GISTs in EUS images is a crucial factor to influence the diagnosis. U-net, an elegant network, has been commonly used in medical images. However, due to the plain architecture and complicated up-sampling path of U-net, classical U-net does not perform well in segmenting GISTs in EUS images with diverse size, heavy shadow and ambiguous boundary. Hence, this paper proposes a novel multi-task refined boundary-supervision U-net (MRBSU-net) for GIST segmentation in EUS images. In our network, multi-task refined U-net (RU-net) is set to deal with heavy shadow and diverse size. Boundary cross entropy in loss function of multi-task RU-net boosts the influence of small size tumors and the refinement avoid the noise information in EUS images propagating to the higher resolution layers. Then we design a refined boundary-supervision U-net (RBSU-net) to solve the ambiguous problem. The boundary supervision in RBSU-net leads the network focus on finding boundary in the down-sampling part and segmenting region on the up-sampling path. At last, we put multi-task RU-net in front of the RBSU-net to increase the stability of the network, what is called MRBSU-net. Extensive experiments have been designed to evaluate the performance of the proposed network. The comparison experiments include the results from traditional U-net, generative adversarial network (GAN) and Deep Attentional Features (DAF). The results of our proposed method perform best among all the comparison methods, which proves that the proposed network could be potentially used in clinic.
- Published
- 2020
24. Endoscopic variceal ligation combined with sclerotherapy for management of gastroesophageal variceal bleeding in pediatric patients
- Author
-
Feng Shen, Fang Sun, Ming-Ming Li, Man-Xiu Huai, Leiming Xu, Yi Zhang, and Chun-Ying Qu
- Subjects
medicine.medical_specialty ,Variceal bleeding ,business.industry ,medicine.medical_treatment ,medicine ,Sclerotherapy ,Ligation ,business ,Surgery - Abstract
Background Portal hypertension (PH) frequently gives rise to severe and life-threatening complications, including hemorrhage accompanied by the rupture of esophageal and gastric varices. In contrast to the widely studied guidelines for the management of PH in adults, there remains controversial about the optimal endoscopic management of variceal bleeding in children for secondary prophylaxis. The study aims to determine the efficacy and safety of endoscopic variceal ligation (EVL) and sclerotherapy (EST) to control bleeding in children in our clinical center. Methods The retrospective study included 21 children with gastroesophageal variceal bleeding who were treated by endoscopic variceal ligation or sclerotherapy at Xinhua Hospital, Shanghai Jiaotong University School of Medicine between January 2007 and July 2020. The treatment outcome involving short-term hemostatic rate and long-term rebleeding rate were investigated. Adverse events related to the procedures, such as esophageal ulcer, esophageal stricture, abnormal embolization, pneumonia and perforation were also observed. Results The 21 pediatric patients who were diagnosed as moderate to severe esophageal varices concurrent with gastric varices experienced EVL or EST successfully. Hemostasis was achieved in 45 of 47 (95.7%) episodes of upper gastrointestinal bleeding. The mean volume of each single aliquot of cyanoacrylate injected was 0.3 ± 0.1 mL (range: 0.1–0.5 mL). Twenty-four patients (75%) reach varices eradication in the EVL group with a median number of procedures before eradication of 2 (1–4) and a median time to eradication of 3.40 months (1.10-13.33). Eleven patients (52.4%) developed rebleeding events, with the mean duration of hemostasis being 11.1 ± 11.6 mo (range: 1.0-39.2 mo). No treatment-related complications, for example, distal embolism, were noted with the exception of abdominal pain in one patient (4.8%). Conclusions Endoscopic variceal sclerotherapy or in combination with EVL turns out to be an effective and safe approach to treat variceal hemorrhage in children for secondary prophylaxis.
- Published
- 2021
25. TM4SF1 Promotes Gemcitabine Resistance of Pancreatic Cancer In Vitro and In Vivo.
- Author
-
Jia Cao, Jiachun Yang, Vijaya Ramachandran, Thiruvengadam Arumugam, Defeng Deng, Zhaoshen Li, Leiming Xu, and Craig D Logsdon
- Subjects
Medicine ,Science - Abstract
BACKGROUND:TM4SF1 is overexpressed in pancreatic ductal adenocarcinoma (PDAC) and affects the development of this cancer. Also, multidrug resistance (MDR) is generally associated with tumor chemoresistance in pancreatic cancer. However, the correlation between TM4SF1 and MDR remains unknown. This research aims to investigate the effect of TM4SF1 on gemcitabine resistance in PDAC and explore the possible molecular mechanism between TM4SF1 and MDR. METHODS:The expression of TM4SF1 was evaluated in pancreatic cancer cell lines and human pancreatic duct epithelial (HPDE) cell lines by quantitative RT-PCR. TM4SF1 siRNA transfection was carried out using Hiperfect transfection reagent to knock down TM4SF1. The transcripts were analyzed by quantitative RT-PCR, RT-PCR and western blotting for further study. The cell proliferation and apoptosis were obtained to investigate the sensitivity to gemcitabine of pancreatic cancer cells after silencing TM4SF1 in vitro. We demonstrated that cell signaling of TM4SF1 mediated chemoresistance in cancer cells by assessing the expression of multidrug resistance (MDR) genes using quantitative RT-PCR. In vivo, we used orthotopic pancreatic tumor models to investigate the effect of proliferation after silencing TM4SF1 by a lentivirus-mediated shRNA in MIA PaCa-2 cell lines. RESULTS:The mRNA expression of TM4SF1 was higher in seven pancreatic cancer cell lines than in HPDE cell lines. In three gemcitabine-sensitive cell lines (L3.6pl, BxPC-3, SU86.86), the expression of TM4SF1 was lower than that in four gemcitabine-resistant cell lines (MIA PaCa-2, PANC-1, Hs766T, AsPC-1). We evaluated that TM4SF1 was a putative target for gemcitabine resistance in pancreatic cancer cells. Using AsPC-1, MIA PaCa-2 and PANC-1, we investigated that TM4SF1 silencing affected cell proliferation and increased the percentages of cell apoptosis mediated by treatment with gemcitabine compared with cells which were treated with negative control. This resistance was associated with the expression of multidrug resistance genes including ABCB1 and ABCC1. In vivo, silencing of TM4SF1 in MIA PaCa-2 cell lines increased the effectiveness of gemcitabine-based treatment in orthotopic pancreatic tumor models evaluated using noninvasive bioluminescent imaging. CONCLUSION:These findings suggest that TM4SF1 is a surface membrane antigen that is highly expressed in pancreatic cancer cells and increases the chemoresistance to gemcitabine. Thus, TM4SF1 may be a promising target to overcome the chemoresistance of pancreatic cancer.
- Published
- 2015
- Full Text
- View/download PDF
26. A novel channel assignment algorithm based on topology simplification in multi-radio wirelesss mesh networks.
- Author
-
Leiming Xu, Yong Xiang, and Meilin Shi
- Published
- 2006
- Full Text
- View/download PDF
27. Regulating Glucose Metabolism with Prodrug Nanoparticles for Promoting Photoimmunotherapy of Pancreatic Cancer
- Author
-
Haijun Yu, Qiurong Zhu, Leiming Xu, Mingyue Zheng, Tianliang Li, Madiha Saeed, Man-Xiu Huai, Zhiai Xu, Rundi Song, Fang Sun, Feisheng Zhong, and Cen Xie
- Subjects
photoimmunotherapy ,General Chemical Engineering ,medicine.medical_treatment ,glucose metabolism ,pancreatic cancer ,prodrug nanoparticles ,General Physics and Astronomy ,Medicine (miscellaneous) ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Immune system ,Pancreatic cancer ,immunogenic cell death ,medicine ,Cytotoxic T cell ,General Materials Science ,lcsh:Science ,Tumor microenvironment ,Full Paper ,Chemistry ,General Engineering ,Photoimmunotherapy ,Immunotherapy ,Full Papers ,021001 nanoscience & nanotechnology ,medicine.disease ,Immune checkpoint ,0104 chemical sciences ,Cancer research ,Immunogenic cell death ,lcsh:Q ,0210 nano-technology - Abstract
The low immunogenicity, insufficient infiltration of T lymphocytes, and dismal response to immune checkpoint blockade therapy pose major difficulties in immunotherapy of pancreatic cancer. Photoimmunotherapy by photodynamic therapy (PDT) can induce an antitumor immune response by triggering immunogenic cell death in the tumor cells. Notwithstanding, PDT‐driven oxygen consumption and microvascular damage can further aggravate hypoxia to exaggerates glycolysis, leading to lactate accumulation and immunosuppressive tumor microenvironment. Herein, a supramolecular prodrug nanoplatform codelivering a photosensitizer and a prodrug of bromodomain‐containing protein 4 inhibitor (BRD4i) JQ1 for combinatory photoimmunotherapy of pancreatic cancer are demonstrated. The nanoparticles are fabricated by host–guest complexation between cyclodextrin‐grafted hyaluronic acid (HA‐CD) and adamantine‐conjugated heterodimers of pyropheophorbide a (PPa) and JQ1, respectively. HA can achieve active tumor targeting by recognizing highly expressed CD44 on the surface of pancreatic tumors. PPa‐mediated PDT can enhance the immunogenicity of the tumor cells and promote intratumoral infiltration of the cytotoxic T lymphocytes. Meanwhile, JQ1 combats PDT‐mediated immune evasion through inhibiting expression of c‐Myc and PD‐L1, which are key regulators of tumor glycolysis and immune evasion. Collectively, this study presents a novel strategy to enhance photoimmunotherapy of the pancreatic cancer by provoking T cells activation and overcoming adaptive immune resistance., Supramolecular prodrug nanosystem composed of β‐cyclodextrin‐grafted hyaluronic acid and reduction‐activatable adamantine‐JQ1/adamantine‐Pheophorbide A conjugates is reported. The prodrug nanoparticle induces immunogenic cell death in the pancreatic tumor cells under near‐infrared laser irradiation, and consequently promoted intratumoral infiltration of the cytotoxic T lymphocytes. Meanwhile, JQ1 relieves glycolysis‐mediated immune evasion and photodynamic therapy‐triggered PD‐L1 overexpression for durable regression of the pancreatic tumor.
- Published
- 2021
28. Expression Changes of Long Noncoding RNA in the Process of Endothelial Cell Activation
- Author
-
Wen-Jin Ding, Min Zhou, Leiming Xu, Yun-Feng Wei, Yingwei Chen, Feng Shen, Jun-Yi Zeng, and Chun-Ying Qu
- Subjects
0301 basic medicine ,Databases, Factual ,Microarray ,Physiology ,Cellular differentiation ,Activation ,Biology ,lcsh:Physiology ,lcsh:Biochemistry ,03 medical and health sciences ,lncRNA ,Endothelial cell ,Humans ,lcsh:QD415-436 ,Gene Regulatory Networks ,Gene ,Cells, Cultured ,Oligonucleotide Array Sequence Analysis ,Messenger RNA ,lcsh:QP1-981 ,Microarray analysis techniques ,Endothelial Cells ,Long non-coding RNA ,Fold change ,Cell biology ,Endothelial stem cell ,030104 developmental biology ,Gene Expression Regulation ,RNA, Long Noncoding ,Transcriptome ,Expression changes - Abstract
Background: Endothelial cells have been shown to be in response to a variety of local and systemic stimuli, and are able to transition between quiescent and activated states. Endothelial cell activation is critical for the pathogenesis of various cardiovascular diseases. However, the expression changes of long non-coding RNAs (lncRNAs) are still unknown in the process of endothelial cell activation. Thus, this study was aimed to investigate expression changes of lncRNA before and after endothelial cell activation. Materials and Methods: In an experimental model of peripheral venous congestion, endothelial cells were activated and analyzed with Affymetrix HG-U133 plus2.0 microarray. We analyzed these microarray data and reannotated the microarray probes for lncRNA. Results: According to the definition of absolute fold change>2 and p value
- Published
- 2017
29. The combination of wheat peptides and fucoidan protects against chronic superficial gastritis and alters gut microbiota: a double-blinded, placebo-controlled study
- Author
-
Leiming Xu, Meijiao Cheng, Jun Du, Yumin Liu, Juntao Kan, Liang Chen, Junrui Cheng, Molly Hood, and Dingfu Zhong
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,China ,media_common.quotation_subject ,Placebo-controlled study ,Medicine (miscellaneous) ,Chronic gastritis ,030209 endocrinology & metabolism ,Gut flora ,Placebo ,Gastroenterology ,03 medical and health sciences ,Feces ,0302 clinical medicine ,Bloating ,Double-Blind Method ,Polysaccharides ,Internal medicine ,Surveys and Questionnaires ,medicine ,Humans ,Adverse effect ,Triticum ,media_common ,030109 nutrition & dietetics ,Nutrition and Dietetics ,biology ,business.industry ,Appetite ,biology.organism_classification ,medicine.disease ,Anti-Ulcer Agents ,Gastrointestinal Microbiome ,Gastric Mucosa ,Gastritis ,Chronic Disease ,Female ,Calprotectin ,business ,Peptides - Abstract
Chronic gastritis is observed in almost half world population. Traditional medications against chronic gastritis might produce adverse effects, so alternative nutritional strategies are needed to prevent the aggravation of gastric mucosal damage. The aim of this study is to evaluate the protective effect of the combination of wheat peptides and fucoidan (WPF) on adults diagnosed with chronic superficial gastritis in a randomized, double-blind, placebo-controlled clinical trial. Participants were randomized to receive WPF (N = 53) or placebo (N = 53) once daily for 45 days. Pathological grading of gastric mucosal damage was scored using gastroscopy. Fecal samples were collected for the determination of calprotectin, short chain fatty acids (SCFA) levels and metagenomics analysis. Questionnaires for self-reported gastrointestinal discomforts, life quality and food frequency were collected throughout the study. WPF intervention reduced gastric mucosal damage in 70% subjects (P
- Published
- 2019
30. The distribution of trace elements in granitoids in the Nanling Region of China
- Author
-
Zhongmn, Yuan, Chengyi, Wu, Leiming, Xu, and Yunxiang, Ni
- Published
- 1993
- Full Text
- View/download PDF
31. Galanin receptor 2 mediates antifibrogenic effects of galanin on hepatic stellate cells
- Author
-
Lingnan He, Da Zhou, Zhenghong Li, Leiming Xu, Yuanwen Chen, Jian-Gao Fan, and Yongnian Ding
- Subjects
0301 basic medicine ,endocrine system ,Cancer Research ,medicine.medical_specialty ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,Downregulation and upregulation ,Internal medicine ,medicine ,Galanin ,Receptor ,Gene knockdown ,Cell growth ,digestive, oral, and skin physiology ,hemic and immune systems ,Articles ,General Medicine ,030104 developmental biology ,Endocrinology ,nervous system ,Hepatic stellate cell ,030211 gastroenterology & hepatology ,hormones, hormone substitutes, and hormone antagonists ,Galanin receptor 2 ,Transforming growth factor - Abstract
Galanin is an endogenous factor involved in the negative regulation of the biological effects of leptin in bioenergetic metabolism. Leptin promotes fibrogenic effects in hepatic stellate cells (HSCs), however, little is known about the effects of galanin on HSCs. In the present study, the biological functions of galanin and its receptors (GalRs) in HSCs were investigated using cell culture in vitro. It was found that galanin and GalR3 mRNA are expressed in quiescent and activated HSCs. GalR2 expression was undetectable in quiescent HSCs but was markedly induced in activated HSCs. In the HSC-T6 cell line, which is an activated rat HSC cell line, treatment with 100 nmol/l galanin significantly inhibited cell proliferation. It also inhibited transforming growth factor (TGF)-β1 and α-smooth muscle actin (SMA) expression and upregulated peroxisome proliferator-activated receptor (PPAR)-γ expression. Following the knockdown of GalR2 by specific small interfering RNA, the activation of GalR3 by galanin does not influence these effects of galanin on HSCs. However, activation of GalR2 alone by galanin following the knockdown of GalR3 inhibits HSC proliferation and TGF-β1 and α-SMA expression, in addition to inducing PPAR-γ expression. These data suggest that galanin inhibits HSC activation and suppresses the profibrogenic features of these cells, and these effects might be mediated by GalR2. Thus, galanin is a potential endogenous factor in the inhibition of liver fibrosis.
- Published
- 2016
32. Epithelioid hemangioma of bone: a report of two special cases and a literature review
- Author
-
YanBiao Fu, Liangji Lu, Qin Zhou, Leiming Xu, and KeWei Xiang
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Radiography ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,Scapula ,Intermediate Vascular Neoplasm ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Epithelioid Hemangioma ,medicine.diagnostic_test ,business.industry ,Magnetic resonance imaging ,Swollen lymph nodes ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Hemangioendothelioma, Epithelioid ,Female ,Radiology ,Neoplasm Recurrence, Local ,Differential diagnosis ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Cervical vertebrae - Abstract
Intraosseous epithelioid hemangioma (EH) is a rare intermediate vascular neoplasm, characterized by locally aggressive and rarely metastasizing behavior. Occasionally, EH of bone can behave strangely and may simulate malignant neoplasm. Here, we report two cases of EH of bone. Of interest was the fact that the computed tomography and magnetic resonance images from one case showed an osteolytic lesion in the right scapula, with multiple swollen lymph nodes in the right supraclavicular and axillary areas. Another patient exhibited a local recurrence in the cervical vertebrae. The initial radiological diagnosis of both cases was metastatic tumor. EH should be included in the differential diagnosis of a radiographic osteolytic lesion with an aggressive appearance. Also, we reviewed the literature that reported EH of bone and summarized their radiological appearances. The cases of EH of bone that exhibited involvement of regional or draining lymph nodes were also summarized.
- Published
- 2016
33. Low Cost and Solution Processed Interfacial Layer Based on Poly(2-ethyl-2-oxazoline) Nanodots for Inverted Perovskite Solar Cells
- Author
-
Yudong Zhu, Wei Chen, Leiming Xu, Gui-ning Zhang, Yizhe Yu, and Zhubing He
- Subjects
Materials science ,General Chemical Engineering ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,2-ethyl-2-oxazoline ,0104 chemical sciences ,Solution processed ,Materials Chemistry ,Nanodot ,0210 nano-technology ,Layer (electronics) ,Perovskite (structure) - Published
- 2016
34. Novel Molecular Doping Mechanism for n-Doping of SnO
- Author
-
Bao, Tu, Yangfan, Shao, Wei, Chen, Yinghui, Wu, Xin, Li, Yanling, He, Jiaxing, Li, Fangzhou, Liu, Zheng, Zhang, Yi, Lin, Xiaoqi, Lan, Leiming, Xu, Xingqiang, Shi, Alan Man Ching, Ng, Haifeng, Li, Lung Wa, Chung, Aleksandra B, Djurišić, and Zhubing, He
- Abstract
Molecular doping of inorganic semiconductors is a rising topic in the field of organic/inorganic hybrid electronics. However, it is difficult to find dopant molecules which simultaneously exhibit strong reducibility and stability in ambient atmosphere, which are needed for n-type doping of oxide semiconductors. Herein, successful n-type doping of SnO
- Published
- 2018
35. Effects of Photodynamic Therapy on Pancreatic Cancer by Using a Potential Photosensitizer (17R, 18R)-2-(1-Hexyloxyethyl)-2-Devinyl Chlorine E6 Trisodium Salt and Its Combination with Simvastatin for Alleviating Metastasis in Vitro and in Vivo
- Author
-
YuJie Shen, Jia Cao, Fang Sun, Xiaolei Cai, Mingming Li, Nannan Zheng, Chunying Qu, Yi Zhanga, Feng Shen, Min Zhou, Yingwei Chen, and Leiming Xu
- Published
- 2018
36. Study on Calculation and Compensation Method of Surface Q in Loess Plateau
- Author
-
Lidong, Gao, primary, Daxing, Wang, additional, Xiaoliang, Liu, additional, Leiming, Xu, additional, and Sijian, Cheng, additional
- Published
- 2018
- Full Text
- View/download PDF
37. Evaluation of three-dimensional arterial spin labeling perfusion imaging for the pathological investigation of musculoskeletal tumors
- Author
-
Lingbin Xu, Haidong Zhu, and Leiming Xu
- Subjects
Cancer Research ,Receiver operating characteristic ,medicine.diagnostic_test ,Correlation coefficient ,business.industry ,Area under the curve ,Magnetic resonance imaging ,Perfusion scanning ,General Medicine ,Blood flow ,Articles ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,030220 oncology & carcinogenesis ,medicine ,Immunohistochemistry ,Nuclear medicine ,business ,Pathological - Abstract
The present study aimed to assess the clinical utility of three-dimensional arterial spin labeling (3D-ASL) perfusion imaging in discriminating between benign, intermediate and malignant musculoskeletal tumors, as well as to analyze the correlation between tumor blood flow (TBF) and microvessel density (MVD). 3D-ASL was performed on 44 patients with musculoskeletal tumor using a 3.0-T magnetic resonance system to obtain TBF values prior to surgery. TBF was independently measured by two radiologists. The TBF values of different groups were compared by one-way analysis of variance. A receiver operating characteristic (ROC) curve was applied to assess the threshold and diagnostic reliability of TBF. Immunohistochemical staining of tumor specimens was performed using a cluster of differentiation 34 monoclonal antibody to calculate MVD counts. The correlation between TBF and MVD counts was analyzed using correlative analysis. Pathology results for a total of 44 cases were obtained by surgery. Good interobserver agreement was found for the TBF values independently determined by the two radiologists (intra-class correlation coefficient test, 0.891; P
- Published
- 2017
38. Novel Molecular Doping Mechanism for n‐Doping of SnO 2 via Triphenylphosphine Oxide and Its Effect on Perovskite Solar Cells
- Author
-
Yinghui Wu, Aleksandra B. Djurišić, Wei Chen, Yanling He, Leiming Xu, Zhubing He, Jiaxing Li, Yi Lin, Alan Man Ching Ng, Xingqiang Shi, Bao Tu, Xiaoqi Lan, Zheng Zhang, Fangzhou Liu, Yangfan Shao, Lung Wa Chung, Haifeng Li, and Xin Li
- Subjects
Materials science ,Dopant ,business.industry ,Mechanical Engineering ,Doping ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Delocalized electron ,chemistry.chemical_compound ,Semiconductor ,chemistry ,Mechanics of Materials ,Chemical physics ,General Materials Science ,Work function ,0210 nano-technology ,business ,Tin ,Triphenylphosphine oxide ,Perovskite (structure) - Abstract
Molecular doping of inorganic semiconductors is a rising topic in the field of organic/inorganic hybrid electronics. However, it is difficult to find dopant molecules which simultaneously exhibit strong reducibility and stability in ambient atmosphere, which are needed for n-type doping of oxide semiconductors. Herein, successful n-type doping of SnO2 is demonstrated by a simple, air-robust, and cost-effective triphenylphosphine oxide molecule. Strikingly, it is discovered that electrons are transferred from the R3P+ O- σ-bond to the peripheral tin atoms other than the directly interacted ones at the surface. That means those electrons are delocalized. The course is verified by multi-photophysical characterizations. This doping effect accounts for the enhancement of conductivity and the decline of work function of SnO2 , which enlarges the built-in field from 0.01 to 0.07 eV and decreases the energy barrier from 0.55 to 0.39 eV at the SnO2 /perovskite interface enabling an increase in the conversion efficiency of perovskite solar cells from 19.01% to 20.69%.
- Published
- 2019
39. Synergy Effect of Both 2,2,2-Trifluoroethylamine Hydrochloride and SnF2 for Highly Stable FASnI3−x Clx Perovskite Solar Cells
- Author
-
Fangzhou Liu, Leiming Xu, Yudong Zhu, Zhubing He, Wei Chen, Xusheng Zhang, Yi Lin, Yinghui Wu, Zhenfei He, Aleksandra B. Djurišić, Min Liao, Jie Ding, Bao Tu, Bin-Bin Yu, Weitang Yao, and Jin-Song Hu
- Subjects
chemistry.chemical_compound ,Materials science ,chemistry ,Hydrochloride ,Trifluoroethylamine ,Inorganic chemistry ,Energy Engineering and Power Technology ,Electrical and Electronic Engineering ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,Perovskite (structure) - Published
- 2019
40. Easy and Effective Endoscopic Retrieval of Ingested Sharp Foreign Bodies
- Author
-
null Leiming Xu, null Chao Huang, null Chunying Qu, null Yi Zhang, null Min Zhou, and null Ying Chen
- Subjects
Foreign Bodies - Abstract
Here we reported two cases of successful removal of accidentally ingested sharp foreign body. The swallowed objects were dentures with clasps which increased the risk of gastroesophageal bleeding or perforation in both cases. After several unsuccessful attempts of removal with normal assistant apparatus, the foreign bodies were eventually retrieved by using modified ship-form semitransparent plastic bags without any complications.
- Published
- 2013
41. Effects of arginineglycineaspartic acid peptide conjugated quantum dots induced photodynamic therapy on pancreatic carcinoma in vivo
- Author
-
Leiming Xu
- Published
- 2016
42. MicroRNA-221-3p Plays an Oncogenic Role in Gastric Carcinoma by Inhibiting PTEN Expression
- Author
-
Yuqin Li, Leiming Xu, Yi Zhang, Shengtian Wu, Jianping Shi, and Nuyun Jin
- Subjects
0301 basic medicine ,Oncology ,Male ,Cancer Research ,PTEN ,Cell Cycle Proteins ,medicine.disease_cause ,Metastasis ,Mice ,Phosphatidylinositol 3-Kinases ,0302 clinical medicine ,RNA interference ,3' Untranslated Regions ,biology ,General Medicine ,Prognosis ,miR-221-3p ,Gene Expression Regulation, Neoplastic ,Cell Transformation, Neoplastic ,030220 oncology & carcinogenesis ,Disease Progression ,RNA Interference ,Signal transduction ,Signal Transduction ,medicine.medical_specialty ,Article ,03 medical and health sciences ,Stomach Neoplasms ,Internal medicine ,Cell Line, Tumor ,microRNA ,medicine ,Gene silencing ,Animals ,Humans ,Adaptor Proteins, Signal Transducing ,Cell Proliferation ,Cell growth ,Akt ,PTEN Phosphohydrolase ,Gastric carcinoma ,medicine.disease ,Phosphoproteins ,Xenograft Model Antitumor Assays ,Disease Models, Animal ,MicroRNAs ,030104 developmental biology ,biology.protein ,Cancer research ,Carcinogenesis ,Proto-Oncogene Proteins c-akt - Abstract
Gastric carcinoma is one of the most common malignancies in men, and microRNA plays a critical role in regulating the signaling networks of gastric carcinoma tumorigenesis and metastasis. We first report the functional characteristics of miR-221-3p in gastric carcinoma. Quantification in gastric carcinoma cell lines and tumor samples reveals significantly increasing miR-221-3p expression. Moreover, a high level of miR-221-3p is correlated with a poor prognosis for gastric carcinoma patients. Ectopic miR-221-3p expression significantly promotes gastric carcinoma cell proliferation, invasion, and sphere formation, while silencing miR-221-3p significantly inhibits these abilities in gastric carcinoma cells. Tests in vivo showed that miR-221-3p significantly promotes tumor growth in xenograft mouse models. In this study, we reveal that miR-221-3p targets PTEN mRNA and downregulates PTEN, which is the possible mechanism of miR-221-3p-induced oncogenic properties. Collectively, we reveal a critical role for miR-221-3p in gastric carcinoma development and progression.
- Published
- 2016
43. Metal Acetylacetonate Series in Interface Engineering for Full Low-Temperature-Processed, High-Performance, and Stable Planar Perovskite Solar Cells with Conversion Efficiency over 16% on 1 cm
- Author
-
Wei, Chen, Leiming, Xu, Xiyuan, Feng, Jiansheng, Jie, and Zhubing, He
- Abstract
A series of metal acetylacetonates produced by a full low-temperature (below 100 °C) process are successfully employed to obtain both "multistable" and high-performance planar-inverted perovskite solar cells. All the three kinds of champion cells in small area exhibit over 18% in conversion-efficiency with negligible hysteresis, along with a conversion efficiency above 16% for planar PSCs in an aperture area of over 1 cm
- Published
- 2016
44. NEAT1 regulates pancreatic cancer cell growth, invasion and migration though mircroRNA-335-5p/c-met axis
- Author
-
Jia, Cao, Yi, Zhang, Jiachun, Yang, Sijia, He, Mingming, Li, Shiyan, Yan, Ying, Chen, Chunying, Qu, and Leiming, Xu
- Subjects
Original Article - Abstract
NEAT1 has been reported to affect cancer progression, which was subsequently confirmed in multiple cancers. Hsa-miRNA-335-5p (miR-335-5p) has recently been identified as an anticancer agent in various organs. However, the relationship between NEAT1 and miR-335-5p remains poorly understood. In this study, we investigated the effects of NEAT1 and miR-335-5p on development of pancreatic cancer. The ectopic expression of miR-335-5p in pancreatic cancer cell lines significantly suppressed cell growth by inhibiting c-met. In addition, downregulating NEAT1 upregulates miR-335-5p. Taken together, our results demonstrate that the NEAT1/miR-335-5p/c-met axis plays a pivotal role in pancreatic cancer by regulating the proliferation, metastasis, and apoptosis of pancreatic cancer cells in vivo and in vitro.
- Published
- 2016
45. Robust interface engineering for High-performance and Stable inverted planar perovskite solar cells via both poly(2-ethyl-2-oxazoline) nanodots
- Author
-
Zhubing He, Leiming Xu, and Wei Chen
- Subjects
Materials science ,business.industry ,Optoelectronics ,Work function ,Quantum efficiency ,Nanotechnology ,Heterojunction ,Nanodot ,business ,Solar energy ,Ohmic contact ,Layer (electronics) ,Perovskite (structure) - Abstract
Low temperature, stable and facile solution processes are the typical characters in mass production of next generation solar cells, especially for flexible consumable electronics. In this work, we employed a low cost, chemical stable, solution processing polymer, thermal treatment free poly(2-ethyl-2-oxazoline) (PEOz) nanodots, as an efficient interfacial layer on top of [6,6]-phenyl-C61-butyricacid methyl (PCBM) to fabricate inverted planar heterojunction (PHJ) perovskite solar cells. The performance of perovskite solar cells increased from 9.01% to 14.52% after inserting a thin layer of PEOz between PCBM and Ag. It was demonstrated that PEOz can effectively reduce the work function of silver electrode to form an ohmic contact between PCBM and silver, which dramatically enhance the electron extraction capability from PCBM to silver electrode. This study pays a way for the large scale fabrication of high efficiency perovskite thin film solar cells via low cost solution processing at low temperatures.
- Published
- 2016
46. miR-200b is involved in intestinal fibrosis of Crohn’s disease
- Author
-
Chun-Ying Qu, Mingjie Zhu, Wensong Ge, Yongtao Xiao, Yingwei Chen, Wenzhu Zhang, and Leiming Xu
- Subjects
Crohn’s disease ,medicine.medical_specialty ,diagnosis ,epithelial-mesenchymal transition ,Down-Regulation ,Biology ,Gastroenterology ,Transforming Growth Factor beta1 ,Pathogenesis ,Crohn Disease ,Fibrosis ,Internal medicine ,Genetics ,medicine ,Humans ,Intestinal Mucosa ,Cells, Cultured ,Crohn's disease ,microRNA ,Oncogene ,fibrosis ,Cancer ,Epithelial Cells ,Articles ,General Medicine ,Cell cycle ,medicine.disease ,Molecular medicine ,Intestines ,MicroRNAs ,Immunology ,Transforming growth factor - Abstract
Intestinal fibrosis is one of the major serious complications of Crohn's disease (CD). However, there are no effective antifibrotic drugs to treat intestinal fibrosis in CD. Therefore, it is important to understand the pathogenesis of fibrosis in CD. It has been reported that members of the miR-200 family are essential in the regulation of renal fibrogenesis. In this study, we analyzed the function of miR-200a and miR-200b in intestinal fibrosis, which was induced by transforming growth factor β1 (TGF-β1) in vitro. Furthermore, we detected the expression of miR-200a and miR-200b in CD specimens, which were divided into groups of fibrosis and no-fibrosis. The results of this study showed that administration of miR-200b could partially protect intestinal epithelial cells from fibrogenesis in vitro. Furthermore, we found that miR-200b was overexpressed in the serum of the fibrosis group. The results suggest that miR-200b has potential value for diagnostic and therapeutic applications for CD patients with fibrosis complications.
- Published
- 2012
47. Erratum to: ‘Effects of milk containing only A2 beta casein versus milk containing both A1 and A2 beta casein proteins on gastrointestinal physiology, symptoms of discomfort, and cognitive behavior of people with self-reported intolerance to traditional cows’ milk’
- Author
-
Jianqin, Sun, Leiming, Xu, Lu, Xia, Yelland, Gregory W., Ni, Jiayi, and Clarke, Andrew
- Subjects
Adult ,Male ,Medicine (miscellaneous) ,Feces ,Cognition ,Lactose Intolerance ,Asian People ,Double-Blind Method ,Animals ,Humans ,Aged ,Inflammation ,Cross-Over Studies ,Nutrition and Dietetics ,Caseins ,Middle Aged ,Peptide Fragments ,Gastrointestinal Tract ,Milk ,Cattle ,Digestion ,Female ,Endorphins ,Self Report ,Erratum ,Biomarkers - Abstract
Cows' milk generally contains two types of β-casein, A1 and A2 types. Digestion of A1 type can yield the peptide β-casomorphin-7, which is implicated in adverse gastrointestinal effects of milk consumption, some of which resemble those in lactose intolerance. This study aimed to compare the effects of milk containing A1 β-casein with those of milk containing only A2 β-casein on inflammation, symptoms of post-dairy digestive discomfort (PD3), and cognitive processing in subjects with self-reported lactose intolerance.Forty-five Han Chinese subjects participated in this double-blind, randomized, 2 × 2 crossover trial and consumed milk containing both β-casein types or milk containing only A2 β-casein. Each treatment period was 14 days with a 14-day washout period at baseline and between treatment periods. Outcomes included PD3, gastrointestinal function (measured by smart pill), Subtle Cognitive Impairment Test (SCIT), serum/fecal laboratory biomarkers, and adverse events.Compared with milk containing only A2 β-casein, the consumption of milk containing both β-casein types was associated with significantly greater PD3 symptoms; higher concentrations of inflammation-related biomarkers and β-casomorphin-7; longer gastrointestinal transit times and lower levels of short-chain fatty acids; and increased response time and error rate on the SCIT. Consumption of milk containing both β-casein types was associated with worsening of PD3 symptoms relative to baseline in lactose tolerant and lactose intolerant subjects. Consumption of milk containing only A2 β-casein did not aggravate PD3 symptoms relative to baseline (i.e., after washout of dairy products) in lactose tolerant and intolerant subjects.Consumption of milk containing A1 β-casein was associated with increased gastrointestinal inflammation, worsening of PD3 symptoms, delayed transit, and decreased cognitive processing speed and accuracy. Because elimination of A1 β-casein attenuated these effects, some symptoms of lactose intolerance may stem from inflammation it triggers, and can be avoided by consuming milk containing only the A2 type of beta casein.ClinicalTrials.gov/NCT02406469.
- Published
- 2015
48. Clinical study of autofluorescence imaging combined with narrow band imaging in diagnosing early gastric cancer and precancerous lesions
- Author
-
Jianping, Shi, Nuyun, Jin, Yuqin, Li, Shuangqin, Wei, and Leiming, Xu
- Subjects
Male ,Narrow Band Imaging ,Stomach Neoplasms ,Gastroscopy ,Optical Imaging ,Humans ,Female ,Middle Aged ,Precancerous Conditions ,Aged - Abstract
To evaluate the diagnostic values of autofluorescence imaging (AFI) combined with narrow band imaging (NBI) in diagnosing early gastric cancer and precancerous lesions.140 patients were investigated for lesions such as gastric mucosal roughness, erosion, plaque, abnormal color, bump or pitting by conventional endoscopy. AFI endoscope was used for the observation, and the endoscopic diagnosis was performed according to the fluorescence forms under the endoscopic AFI mode, as well as the changes of gastric mucosal capillaries and gastric pits under the NBI mode. The corresponding lesions were also biopsied for pathological examination.78 patients were diagnosed as gastritis (including superficial and atrophic gastritis), 45 as intestinal metaplasia, 6 as dysplasia, and 11 as early gastric cancer. The sensitivity and specificity of AFI-NBI combination were 88.89 and 91.58% in the diagnosis of intestinal metaplasia; 83.33 and 98.51% for dysplasia; and 90.91 and 99.22% for early gastric cancer. All of them were significantly higher than the simple fluorescence endoscopy.The AFI-NBI combination could improve the detection rate of early gastric cancer and precancerous lesions.
- Published
- 2015
49. Fault growth and linkage: Implications for tectonosedimentary evolution in the Chezhen Basin of Bohai Bay, eastern China
- Author
-
Zhiqiang Wang, Jinbao Su, Jia Wei, Zhiyong Zhang, Wenbin Zhu, Yanfeng Yang, and Leiming Xu
- Subjects
geography ,geography.geographical_feature_category ,Rift ,Tectonostratigraphy ,Energy Engineering and Power Technology ,Geology ,Structural basin ,Fault (geology) ,Fault scarp ,Strike-slip tectonics ,Sedimentary depositional environment ,Paleontology ,Fuel Technology ,Geochemistry and Petrology ,Earth and Planetary Sciences (miscellaneous) ,Paleogene - Abstract
The Chezhen Basin has highly representative structural features of the Jiyang depression, Bohai Bay area. The structural geometry and the boundary fault linkage exert a strong influence on basin development and depositional environment. Based on structural analysis, at least six early fault segments are identified in the northern boundary of the Chezhen Basin. These fault segments are important in controlling stratal architecture and distribution. The antecedent structures controlled subbasin initiation and development. The Cenozoic rift initiated in the early Eocene with the development of six isolated fault segments associated with deposition of the Es4 member. During the deposition of the lower Es3 member, these six fault segments quickly linked and formed the present architecture frame. Fault linkage has not resulted in a redistribution of displacement. With the expansion of the Chezhen Basin, the depocenters of the upper strata were kept in nearly the same sites until the early Miocene, then the activity of the Chengnan fault ceased. Fault linkage is a significant event in basin evolution, and its process may be very rapid. However, the fault linkage exerted considerable control on sedimentation and evolution of the basin. This study demonstrates that it is necessary to integrate structural and stratigraphic data to reconstruct the temporal and spatial evolution of normal fault zones.
- Published
- 2011
50. [A multi-center clinical study of a novel controlled attenuation parameter for assessment of fatty liver]
- Author
-
Feng, Shen, Ruidan, Zheng, Yuqiang, Mi, Junping, Shi, Guofeng, Chen, Jianneng, Chen, Liang, Xu, Qin, Pan, Leiming, Xu, and Jiangao, Fan
- Subjects
Adult ,Male ,China ,Tissue Extracts ,Biopsy ,Middle Aged ,Body Mass Index ,Fatty Liver ,Hepatitis B, Chronic ,ROC Curve ,Area Under Curve ,Multivariate Analysis ,Linear Models ,Bile ,Humans ,Female - Abstract
To evaluate the controlled attenuation parameter (CAP) assessment of fatty liver and choose a cut-off value of hepatic steatosis more than 5%.Consecutive patients, 18 years or older, who had undergone percutaneous liver biopsy and CAP measurement were recruited from five liver healthcare centers in China. All enrollees were categorized as hepatic steatosis grade S0 (5%) or S1 (5%). An M-probe equipped FibroScan 502 was used to capture CAP values. Receiver operating characteristic (ROC) curves were plotted, and the areas under (AU) the curves were calculated to determine the diagnostic efficacy. The CAP cut-off values at the optimal thresholds were defined by maximum Youden indices; sensitivity and specificity were also calculated.A total of 332 patients were enrolled in the study, including 67 patients with non-alcoholic fatty liver disease (NAFLD) and 265 with chronic hepatitis B (CHB) viru: infection. The median age (inter quartile range, IQR) of the study cohort was 39.0 (32.0-50.5) years-old. There were 46 males (68.7%) in the NAFLD group, with a median age of 37.0 (28.0-45.0) years-old, and 182 males (68.7%) in the CHB group; the differences between the two groups in median age and male: female ratio did not reach statistical significance. Multivariate linear regression analysis identified steatosis grade and body mass index (BMI) as independently associated with CAP. The median (IQR) CAP values among patients with S0 and S1 grade steatosis were 215.0 (190.0-241.0) dB/m and 294.0 (255.0-325.5) dB/m (P0.001), respectively. For all patients, when BMI was25 kg/m2, the ability of the AUROC of the CAP to discriminate hepatic steatosis more than or equal to 5% was 0.853, and the optimal cut-off value was 244.5 dB/m; however, when BMI≥25 kg/m2, the AUROC was 0.835 and the optimal cut-off value 269.5 dB/m.CAP can identify hepatic steatosis more than or equal to 5% and is applicable for the diagnosis of fatty liver if it is adjusted for BMI.
- Published
- 2015
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.