Search

Your search keyword '"Leigh, Roger"' showing total 362 results
Start Over Author "Leigh, Roger"
362 results on '"Leigh, Roger"'

1. Bringing Open Data to Whole Slide Imaging

Author

Besson, Sébastien, Leigh, Roger, Linkert, Melissa, Allan, Chris, Burel, Jean-Marie, Carroll, Mark, Gault, David, Gozim, Riad, Li, Simon, Lindner, Dominik, Moore, Josh, Moore, Will, Walczysko, Petr, Wong, Frances, Swedlow, Jason R., Hutchison, David, Editorial Board Member, Kanade, Takeo, Editorial Board Member, Kittler, Josef, Editorial Board Member, Kleinberg, Jon M., Editorial Board Member, Mattern, Friedemann, Editorial Board Member, Mitchell, John C., Editorial Board Member, Naor, Moni, Editorial Board Member, Pandu Rangan, C., Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Terzopoulos, Demetri, Editorial Board Member, Tygar, Doug, Editorial Board Member, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Reyes-Aldasoro, Constantino Carlos, editor, Janowczyk, Andrew, editor, Veta, Mitko, editor, Bankhead, Peter, editor, and Sirinukunwattana, Korsuk, editor

Published
2019
Full Text
View/download PDF

2. The role of soluble growth factors and inhibitors in the vascularisation of lymphoid organs

Author

Leigh, Roger, Coles, Mark C., and O' Toole, Peter

Subjects
571.96
Abstract

Lymph nodes contain complex vascular networks composed of lymphatic and blood vessels. The blood vasculature contains specialised high endothelial venules functioning to permit the efficient entry of blood-borne lymphocytes into the node, while the lymphatics contain antigen-presenting cells draining from tissues. In contrast to the well understood cellular interactions and signalling mechanisms driving development of the stromal networks upon which immune cell interactions occur, the processes by which the complex vascular networks develop are poorly characterised. This study aimed to determine the mechanisms by which vascularisation of lymph node anlagen occurs during development. The structure of developing lymph nodes was studied using confocal microscopy to observe the organisation of basement membranes and the different cell types involved in the process. Expression of angiogenesis-related genes was studied using quantitative real-time PCR and microarrays. No evidence for vascularisation of the anlagen was found, though the markers used may not have stained nascent vessels. The lymph sac surrounding the anlagen was shown to exist as a two-layered structure composed of anastomosing blood and lymphatic endothelium. In vitro models of vasculogenesis and angiogenesis were developed utilising human umbilical vein endothelial cells in three dimensional collagen gels, with and without smooth muscle coverage. A spheroid-based model of angiogenesis was used to study the net angiogenic environment in developing E14.5–E17.5 anlagen, which determined that a net anti-angiogenic environment existed at all timepoints in the lymph nodes and thymus, but not skin. Additionally, tensional forces were observed to affect angiogenic sprouting in addition to soluble growth factors. As a consequence of the double-layered lymph sac observed in vitro, and the influence of anti-angiogenic factors and tensional forces observed ex vivo, a model of lymph node development involving anlagen patterning and vascularisation as a result of condensation-induced tensional forces was proposed, complementary to soluble growth factor-driven angiogenesis.

Published
2011

4. Calcium Compartmentation in Arabidopsis Mesophyll Cells, A Mechanism to Regulate Apoplastic Calcium, Photosynthetic Rates and Growth, Involves Low-affinity, High-capacity Ca2+/H+ Antiporters

Author

Conn, Simon J, Gilliham, Matthew, Tyerman, Stephen, Kaiser, Brent, and Leigh, Roger

Subjects
Calcium, Arabidopsis, SiCSA, Photosynthesis, Homeostasis
Abstract

The way calcium (Ca) is stored in plants impacts upon plant, human and animal nutrition. An X-ray microanalysis study of over 40 angiosperm species has highlighted conserved accumulation patterns for Ca across different plant families; Ca is often stored in specific leaf cell-types. For instance, in grass monocots Ca accumulation occurs within vacuoles of epidermal cells whereas in the majority of eudicots Ca is predominantly stored within mesophyll cell vacuoles. To correlate gene expression with Ca accumulation profiles we micropipetted RNA from epidermal and mesophyll cells of the eudicot Arabidopsis thaliana. Cell specific RNA libraries were analysed by microarray and qPCR revealing a number of candidate membrane transporters with greater relative expression in the Ca-rich mesophyll. Knockout mutagenesis of one candidate AtCAX1, a tonoplast-localized Ca2+/H+-antiporter, resulted in no mutant phenotype. When the expression of AtCAX3, a complementing family member was also abolished, this resulted in a reduction in total leaf [Ca] and perturbance of the Ca distribution pattern. The double knockout plant had a 3-fold higher apoplastic [Ca] which correlated with reduced stomatal conductance, photosynthetic rate and consequently growth. Apoplastic [Ca], stomatal conductance and growth rate could be recovered to wild-type levels by reducing [Ca] in the growth medium. We implicate CAX1 and its homologues as major regulators of Ca distribution and apoplastic [Ca] in leaves within and between plant orders. Such information will be helpful for the manipulation of Ca within specific cell types of leafy vegetables as a tool for improving human and animal nutrition.

Published
2009

6. Bringing Open Data to Whole Slide Imaging

Author

Besson, Sébastien, primary, Leigh, Roger, additional, Linkert, Melissa, additional, Allan, Chris, additional, Burel, Jean-Marie, additional, Carroll, Mark, additional, Gault, David, additional, Gozim, Riad, additional, Li, Simon, additional, Lindner, Dominik, additional, Moore, Josh, additional, Moore, Will, additional, Walczysko, Petr, additional, Wong, Frances, additional, and Swedlow, Jason R., additional

Published
2019
Full Text
View/download PDF

13. Ion Homeostasis

Author

Amtmann, Anna, Leigh, Roger, Pareek, Ashwani, editor, Sopory, S.K., editor, and Bohnert, Hans J., editor

Published
2010
Full Text
View/download PDF

14. Publishing and sharing multi-dimensional image data with OMERO

Author

Burel, Jean-Marie, Besson, Sébastien, Blackburn, Colin, Carroll, Mark, Ferguson, Richard K., Flynn, Helen, Gillen, Kenneth, Leigh, Roger, Li, Simon, Lindner, Dominik, Linkert, Melissa, Moore, William J., Ramalingam, Balaji, Rozbicki, Emil, Tarkowska, Aleksandra, Walczysko, Petr, Allan, Chris, Moore, Josh, and Swedlow, Jason R.

Published
2015
Full Text
View/download PDF

19. Preface

Author

White, Philip J., Leigh, Roger A., and Pyke, Kevin

Published
2007

Catalog

Books, media, physical & digital resources
See catalog results