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As the global population of older persons increases, age-related medical conditions will have a greater impact on public health. DXA-derived bone and soft tissue metrics are associated with adverse clinical events in aging persons. This study aims to investigate the regional body composition of the appendices by whole-body DXA scans, and the age-related relationships between measures of bone and soft tissue in healthy Caucasian females of a Greek origin residing in the Mediterranean area. Body composition of the legs and the arms was analyzed, and lean mass (LM) and fat mass (FM) metrics were calculated in 330 women aged 20–85 years, using DXA. Peak bone mineral density (BMD) of the legs and arms was achieved between ages 20–30 and 41–50 years, respectively. The overall BMD reduction with age was for the legs 43% and the arms 32.2% (p < 0.001). Peak %LM of the legs and the arms was achieved between ages 20–30. The overall reduction of %LM with age was for the legs 22.5% (p < 0.001) and arms 6.6% (p < 0.05). Peak %FM of the legs and arms was attained between ages 31–40 and 61–70, respectively. The overall %FM reduction with age was for the legs and arms 7.5% and 1.9% (p > 0.05). In appendicular sites, Greek women reach peak values of bone mass in the legs first, in early adulthood. Bone loss predominates in the legs as women age. Also, with advancing age Greek women show preferential significant decreases of %LM and %FM in the legs as opposed to the arms. Although variation in appendicular bone and soft tissue metrics is present, the implications of variable biological crosstalks among the tissue components as women age may ultimately lay the foundation for future clinical trials aimed at healthy aging. [ABSTRACT FROM AUTHOR]

Background: Musculoskeletal disorders (MSDs) among children are common globally and in India with the prevalence ranging from 4% to 15%. Musculoskeletal symptoms can be due to trauma or growing pains or symptoms of potentially life-threatening conditions such as malignancy, sepsis, and autoimmune disorders. With their indefinite onset and long latent period, early detection of these disorders and prompt treatment reduce the severity of outcomes; hence, screening is necessary. Objective: The objective of this study was to estimate the occurrence and describe the morbidity patterns of MSDs among school children in a selected school cluster of urban Bengaluru using the pediatric Gait, Arms, Legs, and Spine (pGALS) screening tool. Methods: A cross-sectional study was done, which included all the students of one randomly selected school cluster in one ward of urban Bengaluru. The total number of students was 260; 37 students did not get their parental consent, and 29 students were chronically absent. The pGALS tool has demonstrated an excellent sensitivity (around 97%) and specificity (around 98%) at all joints with high acceptability among children and parent/guardian and across cultures. Results: The overall occurrence of MSDs based on the pGALS tool among school children was 46.9% (91/194). Males were the most affected than females (males: 51.6% and females: 48.4%). Children in the age group of 10–15 years were most affected by MSDs (51.6%). Conclusion: The prevalence of MSDs is high among school-going children based on the pGALS screening tool, and this may be due to a recent sports event.

Introduction: Historically, difficult-to-treat areas in psoriasis included face, scalp, folds, genitalia, nails and palmoplantar region. Recent studies have found that lower limbs behave like a “new” difficult-to-treat area, as it can be the only site of residual disease even in patients undergoing biologic therapies. Objectives: We aimed to evaluate whether legs had different response rates and response times to treatment with a new biologic drug, risankizumab, than other body sites. Methods: We conducted a real-life, observational, retrospective, multicentric study, including patients affected by moderate-to-severe psoriasis with legs involvement and undergoing biological therapy with risankizumab for more than 16 weeks. The Psoriasis Area Severity Index (PASI) and Leg-PASI were collected at T0 and at week 16, 28, 40, 52, 64 and 76. Statistical analysis using T student test and linear regression analysis were performed. Results: A total of 124 patients were included. The difference between the improvement percentage respect to baseline was statistically significant at week 16 and 28, demonstrating that Leg-PASI improved less than PASI. From the linear regression it was deduced that the slope is statistically less steep for Leg-PASI than for overall PASI, confirming that this site responds more slowly to the therapy. Conclusions: Leg response to risankizumab appears to differ significantly from other body-sites in the first weeks of treatment, even if after 28 weeks statistical significance is lost. Our preliminary finding suggests that risankizumab can be considered an effective treatment for legs psoriasis, but with longer response times than other areas demonstrating the relative nature of resistance to treatment of this district.

The first winged insects evolved from a wingless ancestor, but details of the transition to a fully-winged morphology remain unclear. Studying extant pterygotes with partial wings, such as the stick insects (order Phasmatodea), may help us to understand such a transition. Here, we address how a series of flight-related morphological parameters may correlate with flight evolution by studying different phasmids representing a volancy continuum ranging from miniaturized to full-sized wings. Variation in phasmid wing shape, venation, wing mass and the mass of flight muscle can be described by specific scaling laws referenced to wing length and wing loading. Also, the mass distribution of the body-leg system is conserved in spite of a wide range of variation in body shape. With reduced wing size and increased wing loading, the longitudinal position of the wing-bearing thoracic segments is shifted closer to the insects' centre of body mass. These results demonstrate complex reconfiguration of the flight system during wing morphological transitions in phasmids, with various anatomical features potentially correlated with reduced flight performance attained with partial wings. Although these data represent phasmid-specific features of the flight apparatus and body plan, the associated scaling relationships can provide insight into the functionality of intermediate conditions between wingless and fully-winged insects more generally. [ABSTRACT FROM AUTHOR]

The wings, legs, and tail in Darwin's finches show many clear adaptations to different types of locomotion used during foraging. We use size scaling to analyse how various characters vary with body mass to clarify dimensional relationships. The selective advantage of a character is judged in terms of energy savings. The wing aspect ratio (4.6–4.9) is very low, so the energy costs for flight are high. Low body mass , low wing loading , and short arm wings in the warbler finch, small tree finch, and small ground finch promote agility and manoeuvrability among vegetation, along with short wings in the warbler finch. Evolution towards a shorter arm wing seems to be favoured in the smaller finch species. Long legs , long toes , and long curved claws are adaptations for climbing/clinging locomotion without tail support (woodpecker finch, small and large tree finches, cactus finch but having short legs). Selection for longer legs seems to act towards a lengthening of the tarsometatarsus. The climbing technique in the woodpecker finch is described. We discuss how the diversification in the beaks relates to the locomotion organs. [ABSTRACT FROM AUTHOR]

Our study was focused on the external features differentiation of two adult species of turtles, including a land tortoise (Testudo graeca graeca) and a freshwater turtle (Trachemys scripta elegans), by gross morphological description of head, carapace, plastron, and tail. Generally, turtles show gross morphological differences according to the ecology and the environment. The carapace and legs are the fascinating noticeable parts showing significant differences between tortoise and turtle as T. graeca graeca shows a small carapace length with noticeable convexity. In contrast, the freshwater turtle has a flattened broad carapace adapted to swimming. At the same time, the legs of T. graeca show multiple keratinized spurs with rigid claws, but T. scripta has legs covered with soft, moist skin and thin claws. The plastron is relatively the same in both species, while the head is unique with a large size and red flap in T. scripta. We conclude that the flat body and legs with broad head are a modification for swimming, while the rigid claws with keratinized outer leg skin are signs of land walking adaptation. [ABSTRACT FROM AUTHOR]

Objective: This study aimed to evaluate the effectiveness of DentalVibe in pain reduction during local anesthetic injection compared to traditional injection in pediatric patients. Method and materials: This cross-over randomized controlled clinical trial included a sample of 60 children, aged 5 to 7 years, who were selected based on the need for local anesthesia for bilateral mandibular pulpotomy treatment. They were randomly allocated into two groups. Each group received two mandibular nerve block injections, with a 2-week interval as the washing out period. At first appointment, mandibular nerve block injection was performed either with vibration using DentalVibe at the injection site or benzocaine gel 20% applied before local anesthetic injection; the alternative technique was used at the second appointment. In each visit subjective pain was evaluated using the Wong-Baker FACES Pain Rating Scale and objective pain was evaluated using the FLACC (Face, Legs, Activity, Cry, Consolability) scale. Results: Assessment using the Wong-Baker FACES Pain Rating Scale showed that the mean pain levels in DentalVibe and traditional injection groups were 0.80 ± 1.34 and 2.60 ± 3.22, respectively. The mean pain levels according to the FLACC scale were 2.20 ± 2.04 and 3.13 ± 2.30 in the DentalVibe and traditional injection groups, respectively. Both scales showed statistically significant differences between the two groups in favor of DentalVibe (P < .001). A positive significant correlation between the two scales in the two interventions was recorded, where the Spearman rho was 0.41 for the DentalVibe group, and 0.52 for traditional injection group (P < .001). Conclusion: Compared to the traditional approach, DentalVibe reduced pain sensation during mandibular nerve block injection in pediatric patients. [ABSTRACT FROM AUTHOR]

To report efficacy and safety of percutaneous electrochemotherapy (ECT) in patients with radiotherapy-resistant metastatic epidural spinal cord compression (MESCC). This retrospective study analyzed all consecutive patients treated with bleomycin-based ECT between February-2020 and September-2022 in a single tertiary referral cancer center. Changes in pain were evaluated with the Numerical Rating Score (NRS), in neurological deficit with the Neurological Deficit Scale, and changes in epidural spinal cord compression were evaluated with the epidural spinal cord compression scale (ESCCS) using an MRI. Forty consecutive solid tumour patients with previously radiated MESCC and no effective systemic treatment options were eligible. With a median follow-up of 5.1 months [1–19.1], toxicities were temporary acute radicular pain (25%), prolonged radicular hypoesthesia (10%), and paraplegia (7.5%). At 1 month, pain was significantly improved over baseline (median NRS: 1.0 [0–8] versus 7.0 [1.0–10], P <.001) and neurological benefits were considered as marked (28%), moderate (28%), stable (38%), or worse (8%). Three-month follow-up (21 patients) confirmed improved over baseline (median NRS: 2.0 [0–8] versus 6.0 [1.0–10], P <.001) and neurological benefits were considered as marked (38%), moderate (19%), stable (33.5%), and worse (9.5%). One-month post-treatment MRI (35 patients) demonstrated complete response in 46% of patients by ESCCS, partial response in 31%, stable disease in 23%, and no patients with progressive disease. Three-month post-treatment MRI (21 patients) demonstrated complete response in 28.5%, partial response in 38%, stable disease in 24%, and progressive disease in 9.5%. This study provides the first evidence that ECT can rescue radiotherapy-resistant MESCC. • Radiotherapy-resistant metastatic spinal cord compression is an unmet medical need. • Electrochemotherapy (ECT) improves neurological in this advanced cancer setting. • ECT also results in significant pain decrease at 1-month and 3-months. • At MRI, 77% of patients had an objective response at 1 month and 66.5% at 3 months. • Complications were overall minor, with the exception of paraplegia in 7.5% of patients. [ABSTRACT FROM AUTHOR]

Simple Summary: Insects of the Nepomorpha infraorder live in various environments—some are associated strictly with water and some are mostly terrestrial. In this study, we examined specimens from the aquatic Corixidae and two riparian families—Ochteridae and Gelastocoridae. Apart from the differences in general leg morphology (legs adapted for swimming in Corixidae and for walking in Ochteroidea), there were eight types of sensilla found on the legs. From those, mechanosensilla displayed the most observed variability. The leg sensilla of Nepomorpha had not been studied until now. The aim of this study was to analyze morphological types and arrangement of the leg sensilla of Corixidae, Ochteridae and Gelastocoridae, in relation to their various habitats. The leg sensilla of four species of Corixidae, six of Gelastocoridae and two of Ochteridae were studied. Eight main types of sensilla with six subtypes of sensilla trichodea and four subtypes of sensilla chaetica were found and described. The greatest variability was observed among mechanoreceptive sensilla. The study showed differences in the shape of the legs between strictly aquatic and terrestrial taxa. It is the first attempt to describe leg sensilla among nepomorphan taxa. [ABSTRACT FROM AUTHOR]

Yu-Chuan Chuang,1 Po-Yu Liu,2– 4 Kuo-Lung Lai,5,&ast; Chien-Hao Tseng2,&ast; 1Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan; 2Division of Infectious Disease, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan; 3Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan; 4Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung, Taiwan; 5Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan&ast;These authors contributed equally to this workCorrespondence: Chien-Hao Tseng, Division of Infectious Disease, Department of Internal Medicine, Taichung Veterans General Hospital, No. 1650, Sec. 4, Taiwan Blvd., Xitun Dist, Taichung City, 407219, Taiwan, Tel +886 4 2359 2525 Extension 3081, Email tsengchienhao1116@gmail.comAbstract: Bilateral lower limbs cellulitis is a rare clinical condition, which has been overlooked for a long time. In daily clinical practice, bilateral cellulitis is a sporadically encountered condition; however, it remains a clinical challenge. There is a broad differential diagnosis for this clinical entity, and there is a lack of accepted international diagnostic criteria. Unnecessary antibiotic prescription is common, which has led to an emerging problem. In this review, we summarize case reports of bilateral lower limbs cellulitis and common pathogens that have been documented.Keywords: cellulitis, bilateral, legs, lower limbs, lower extremities

Objective: To bridge the technical gaps in reserve athletes in China’s national boxing program and to rapidly improve the overall level of boxing, the Wushu Sports Administration Center of the General Administration of Sports of China has sent outstanding Sanda players to boxing events through cross-border talent transfer. This was done to widely improve the strengths and resources in various fields to prepare for the Tokyo Olympic Games. In view of this, we analyzed and compared differences in biomechanical parameters of the lead straight punch and index of force developments of the lower extremities between Sanda and boxing. The results provide information and insights to bridge the technical gaps in cross-border talent transfer from Sanda to boxing. Methods: We employed a Vicon infrared 3D motion capture system, two Kistler platforms, a Kistler target, and a synchronous instrument. Eleven boxers and sixteen Sanda athletes were recruited, and their lead straight punch techniques were compared and analyzed. Three indexes of punch velocity, six indexes of strength measurement, and four indexes of lower extremity strength were analyzed. Results: Significant differences in the peak punch velocity and contact velocity were found between the two groups. Furthermore, significant differences were noted in the peak impulse, peak strength, relative strength, and the rate of force development (RFD). Among the kinetics indexes of lower limbs, the front leg strength index was higher in the boxing group than in the Sanda group, namely the RFD index and RFD/ body mass. Conclusion: Based on the disparity in the effects of the lead straight punch and biomechanical parameters of both lower extremities, we can conclude that, compared to the boxers, most Sanda athletes lack standard punching technique. Therefore, it is advised that coaches and practitioners carefully consider selecting Sanda athletes with higher technical standards of punching. [ABSTRACT FROM AUTHOR]

Abstract Background Insecticide-treated bed nets and indoor residual spraying comprise the major control measures against Anopheles gambiae sl, the dominant vector in sub-Saharan Africa. The primary site of contact with insecticide is through the mosquitoes’ legs, which represents the first barrier insecticides have to bypass to reach their neuronal targets. Proteomic changes and leg cuticle modifications have been associated with insecticide resistance that may reduce the rate of penetration of insecticides. Here, we performed a multiple transcriptomic analyses focusing on An. coluzzii legs. Results Firstly, leg-specific enrichment analysis identified 359 genes including the pyrethroid-binder SAP2 and 2 other chemosensory proteins, along with 4 ABCG transporters previously shown to be leg enriched. Enrichment of gene families included those involved in detecting chemical stimuli, including gustatory and ionotropic receptors and genes implicated in hydrocarbon-synthesis. Subsequently, we compared transcript expression in the legs of a highly resistant strain (VK7-HR) to both a strain with very similar genetic background which has reverted to susceptibility after several generations without insecticide pressure (VK7-LR) and a lab susceptible population (NG). Two hundred thirty-two differentially expressed genes (73 up-regulated and 159 down-regulated) were identified in the resistant strain when compared to the two susceptible counterparts, indicating an over-expression of phase I detoxification enzymes and cuticular proteins, with decrease in hormone-related metabolic processes in legs from the insecticide resistant population. Finally, we analysed the short-term effect of pyrethroid exposure on An. coluzzii legs, comparing legs of 1 h-deltamethrin-exposed An. coluzzii (VK7-IN) to those of unexposed mosquitoes (VK7-HR) and identified 348 up-regulated genes including those encoding for GPCRs, ABC transporters, odorant-binding proteins and members of the divergent salivary gland protein family. Conclusions The data on An. coluzzii leg-specific transcriptome provides valuable insights into the first line of defense in pyrethroid resistant and short-term deltamethrin-exposed mosquitoes. Our results suggest that xenobiotic detoxification is likely occurring in legs, while the enrichment of sensory proteins, ABCG transporters and cuticular genes is also evident. Constitutive resistance is primarily associated with elevated levels of detoxification and cuticular genes, while short-term insecticide-induced tolerance is linked with overexpression of transporters, GPCRs and GPCR-related genes, sensory/binding and salivary gland proteins.

Among the different micro- and nanostructures located on cuticular surfaces, grooming devices represent fundamental tools for insect survival. The present study describes the grooming microstructures of the damselfly Ischnura elegans (Odonata, Coenagrionidae) at the adult stage. These structures, situated on the foreleg tibiae, were observed using scanning electron microscopy, and the presence and distribution of resilin, an elastomeric protein that enhances cuticle flexibility, were analyzed using confocal laser scanning microscopy. Eye and antennal grooming behavior were analyzed to evaluate the particle removal efficiency in intact insects and in insects with ablated grooming devices. The grooming devices are constituted of long setae from which a concave cuticular lamina develops towards the medial side of the leg. Each seta shows a material gradient of resilin from its basal to the distal portion and from the seta to the cuticular lamina. The removal of the grooming devices induces a strong increase in the contaminated areas on the eyes after grooming. Further studies on insect grooming can provide valuable data on the functional morphology of insect micro- and nanostructures and can represent a starting point to develop advanced biomimetic cleaning tools., Competing Interests: All the authors declare that they have no conflict of interest., (Copyright © 2024, Piersanti et al.)

Penelitian ini bertujuan untuk mengetahui kontribusi kekuatan otot lengan, daya tahan otot tungkai, dan koordinasi mata tangan terhadap kemapmuan passing bawah dalam permainan bolavoli. Penelitian ini termasuk jenis penelitian deskriptif. Populasi penelitian ini adalah seluruh siswa laki-laki SMK Negeri 2 Watangsoppeng Kabupaten Soppeng dengan jumlah sampel penelitian 40 orang yang dipilih secara random sampling. Teknik analisis data yang digunakan adalah teknik analisis korelasi dengan menggunakan sistem SPSS Versi 22.00 pada taraf signifikan 95% atau a0,05. Bertolak dari hasil analisis data, maka penelitian ini menyimpulkan bahwa: (1) Kekuatan otot lengan memiliki kontribusi terhadap kemampuan passing bawah dalam permainan bolavoli pada siswa SMK Negei 2 Watang Soppeng Kabupaten Soppeng sebesar 53,8%; (2) Daya tahan otot tungkai memiliki kontribusi terhadap kemampuan passing bawah dalam permainan bolavoli pada siswa SMK Negei 2 Watang Soppeng Kabupaten Soppeng sebesar 80,9%; (3) Koordinasi mata tangan memiliki kontribusi terhadap kemampuan passing bawah dalam permainan bolavoli pada siswa SMK Negei 2 Watang Soppeng Kabupaten Soppeng sebesar 52,1%; dan (4) Kekuatan otot lengan, daya tahan otot tungkai dan koordinasi mata tangan memiliki kontribusi terhadap kemampuan passing bawah dalam permainan bolavoli pada siswa SMK Negei 2 Watang Soppeng Kabupaten Soppeng sebesar 81,6%.

Hymenoptera evolved structures on the legs which are able to remove particles from their antennae through grooming behaviour. The antenna cleaner (strigil) consists of an apical and modified protibial spur (calcar, composed of a trunk and a velum) and a modified basitarsus including a fine comb made up of setae and a notched inner surface. In “Terebrantia”, the non-aculeate Apocrita, large comparative studies of strigil are scarce, especially within lineages. Here, we studied in detail this structure in Cynipoidea, a group of wasps including parasitoids (Ibaliidae, Liopteridae and Figitidae), gall-inducers (Cynipidae) and gall-inquilines (Cynipidae), through a SEM analysis. We found some traits quite conserved across species and lineages. For example, the shape of protibia is almost invariably broadening towards apex, and one single, straight rather than curved, dorso-apical socketed spur on apical margin of protibia occurs in almost all species. Other characters roughly differentiate families. For example, thick and long setae on the protibia are arranged in one row especially in Figitidae, Ibaliidae and Liopteridae, while they more often occur in more than one rows in Cynipidae, which was the family with the shortest calcar. Figitidae have shorter basitarsal notch, but had longer and denser setae on the notch, compared with Cynipidae and Ibaliidae. Further characters were extremely variable across and within lineages. The observed morphological variation did not seem to reflect the phylogeny of Cynipoidea, and a role of life-history traits on such variation was not suggested, at least with the methodological approach used here. [ABSTRACT FROM AUTHOR]

Abstract Background Emphasizing the active use of the arms and coordinating them with the stepping motion of the legs may promote walking recovery in patients with impaired lower limb function. Yet, most approaches use seated devices to allow coupled arm and leg movements. To provide an option during treadmill walking, we designed a rope-pulley system that physically links the arms and legs. This arm-leg pulley system was grounded to the floor and made of commercially available slotted square tubing, solid strut channels, and low-friction pulleys that allowed us to use a rope to connect the subject’s wrist to the ipsilateral foot. This set-up was based on our idea that during walking the arm could generate an assistive force during arm swing retraction and, therefore, aid in leg swing. Methods To test this idea, we compared the mechanical, muscular, and metabolic effects between normal walking and walking with the arm-leg pulley system. We measured rope and ground reaction forces, electromyographic signals of key arm and leg muscles, and rates of metabolic energy consumption while healthy, young subjects walked at 1.25 m/s on a dual-belt instrumented treadmill (n = 8). Results With our arm-leg pulley system, we found that an assistive force could be generated, reaching peak values of 7% body weight on average. Contrary to our expectation, the force mainly coincided with the propulsive phase of walking and not leg swing. Our findings suggest that subjects actively used their arms to harness the energy from the moving treadmill belt, which helped to propel the whole body via the arm-leg rope linkage. This effectively decreased the muscular and mechanical demands placed on the legs, reducing the propulsive impulse by 43% (p

Painless legs and moving toes (PoLMT) is a rare variant of painful legs and moving toes (PLMT). The difference is the absence of pain, which is usually hard to manage in PLMT. An involuntary movement in the toes is a compound of both. Although there are many medication options for this disease, most fail to treat it successfully. Here, we report on a 62-year-old female with involuntary movements in her toes for five years. The rarity and unclear etiology of the disease are the reasons we wanted to present it.

Introduction: Physical postures at work and occupational conditions are among the important risk factors that increase the prevalence of varicose veins. These include prolonged standing and tedious postures that are unavoidable in the hairdressing profession. This study aimed to investigate the relationship between the prevalence of foot varicose veins and occupational and demographic factors in hairdressers. Materials and Methods: This cross-sectional-correlational study was conducted on 90 hairdressers in Gonabad in 2015. The participants were selected using a random method. After completing the demographic information questionnaire, the samples were examined according to CEAP criteria (clinical findings, etiology, anatomical findings, and pathophysiology). The collected data were analyzed using Chi-square, Fisher, and independent t-tests using SPSS software version 20 at a significance level of p

The aim of this study was to analyze morphological types and arrangement of the leg sensilla of Corixidae, Ochteridae and Gelastocoridae, in relation to their various habitats. The leg sensilla of four species of Corixidae, six of Gelastocoridae and two of Ochteridae were studied. Eight main types of sensilla with six subtypes of sensilla trichodea and four subtypes of sensilla chaetica were found and described. The greatest variability was observed among mechanoreceptive sensilla. The study showed differences in the shape of the legs between strictly aquatic and terrestrial taxa. It is the first attempt to describe leg sensilla among nepomorphan taxa.

Background: Insecticide-treated bed nets and indoor residual spraying comprise the major control measures against Anopheles gambiae sl, the dominant vector in sub-Saharan Africa. The primary site of contact with insecticide is through the mosquitoes' legs, which represents the first barrier insecticides have to bypass to reach their neuronal targets. Proteomic changes and leg cuticle modifications have been associated with insecticide resistance that may reduce the rate of penetration of insecticides. Here, we performed a multiple transcriptomic analyses focusing on An. coluzzii legs. Results: Firstly, leg-specific enrichment analysis identified 359 genes including the pyrethroid-binder SAP2 and 2 other chemosensory proteins, along with 4 ABCG transporters previously shown to be leg enriched. Enrichment of gene families included those involved in detecting chemical stimuli, including gustatory and ionotropic receptors and genes implicated in hydrocarbon-synthesis. Subsequently, we compared transcript expression in the legs of a highly resistant strain (VK7-HR) to both a strain with very similar genetic background which has reverted to susceptibility after several generations without insecticide pressure (VK7-LR) and a lab susceptible population (NG). Two hundred thirty-two differentially expressed genes (73 up-regulated and 159 down-regulated) were identified in the resistant strain when compared to the two susceptible counterparts, indicating an over-expression of phase I detoxification enzymes and cuticular proteins, with decrease in hormone-related metabolic processes in legs from the insecticide resistant population. Finally, we analysed the short-term effect of pyrethroid exposure on An. coluzzii legs, comparing legs of 1 h-deltamethrin-exposed An. coluzzii (VK7-IN) to those of unexposed mosquitoes (VK7-HR) and identified 348 up-regulated genes including those encoding for GPCRs, ABC transporters, odorant-binding proteins and members of the divergent salivary gland protein family. Conclusions: The data on An. coluzzii leg-specific transcriptome provides valuable insights into the first line of defense in pyrethroid resistant and short-term deltamethrin-exposed mosquitoes. Our results suggest that xenobiotic detoxification is likely occurring in legs, while the enrichment of sensory proteins, ABCG transporters and cuticular genes is also evident. Constitutive resistance is primarily associated with elevated levels of detoxification and cuticular genes, while short-term insecticide-induced tolerance is linked with overexpression of transporters, GPCRs and GPCR-related genes, sensory/binding and salivary gland proteins. [ABSTRACT FROM AUTHOR]

Since various kind of receptors in insects are mainly located on their legs. We estimate the influence of ultraviolet-C on the leg morphology and trichoid sensilla of Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae), zero-day-old pupae were irradiated with UV-C at a distance of 35 cm for 1, 2, 4, 8, 16, 32, and 64 min. Changes in leg morphologies and trichoid sensilla were determined using light and scanning electron microscopes. UV-C radiation caused the abnormalities of an adult's legs that were classified into three levels; mild, moderate, and strong. Legs of the resulting adults were developed into malformed structures that lacked clear segmental identity. The severity of leg abnormalities was recorded in a dosedependent manner. UV-C significantly decreased the length and width of the femur, tibia, and tarsus. In the strongly affected level, the femur was reduced in size while the tibia and tarsus were completely deleted, and there was no evidence of claws. Scanning electron microscopy showed the wrinkled cuticle on the leg segment, and the sensilla of the resulting adults appeared less in number. After UV-C radiation, the trichoid sensilla on the femur became longer and wider, but the trichoid sensilla on the tibia were shorter and narrower. Taken together, UV-C irradiation during pupal stage interfered development of legs and brought about specific malformation features. [ABSTRACT FROM AUTHOR]

Illumina-based transcriptome sequencing of chemosensory organs has become a standard in deciphering the molecular bases of chemical senses in insects, especially in non-model species. A plethora of antennal transcriptomes is now available in the literature, describing large sets of chemosensory receptors and binding proteins in a diversity of species. However, little is still known on other organs such as mouthparts, legs and ovipositors, which are also known to carry chemosensory sensilla. This is the case of the noctuid Spodoptera littoralis, which has been established as a model insect species in molecular chemical ecology thanks to the description of many—but not all—chemosensory genes. To fulfill this gap, we present here an unprecedented transcriptomic survey of chemosensory tissues in this species. RNAseq from male and female proboscis, labial palps, legs and female ovipositors allowed us to annotate 115 putative chemosensory gene transcripts, including 30 novel genes in this species. Especially, we doubled the number of candidate gustatory receptor transcripts described in this species. We also evidenced ectopic expression of many chemosensory genes. Remarkably, one third of the odorant receptors were found to be expressed in the proboscis. With a total of 196 non-overlapping chemosensory genes annotated, the S. littoralis repertoire is one of the most complete in Lepidoptera. We further evaluated the expression of transcripts between males and females, pinpointing sex-specific transcripts. We identified five female-specific transcripts, including one odorant receptor, one gustatory receptor, one ionotropic receptor and one odorant-binding protein, and one male-specific gustatory receptor. Such sex-biased expression suggests that these transcripts participate in sex-specific behaviors, such as host choice for oviposition in females and/or mating partner recognition in both sexes.

Background: Emphasizing the active use of the arms and coordinating them with the stepping motion of the legs may promote walking recovery in patients with impaired lower limb function. Yet, most approaches use seated devices to allow coupled arm and leg movements. To provide an option during treadmill walking, we designed a rope-pulley system that physically links the arms and legs. This arm-leg pulley system was grounded to the floor and made of commercially available slotted square tubing, solid strut channels, and low-friction pulleys that allowed us to use a rope to connect the subject's wrist to the ipsilateral foot. This set-up was based on our idea that during walking the arm could generate an assistive force during arm swing retraction and, therefore, aid in leg swing.Methods: To test this idea, we compared the mechanical, muscular, and metabolic effects between normal walking and walking with the arm-leg pulley system. We measured rope and ground reaction forces, electromyographic signals of key arm and leg muscles, and rates of metabolic energy consumption while healthy, young subjects walked at 1.25 m/s on a dual-belt instrumented treadmill (n = 8).Results: With our arm-leg pulley system, we found that an assistive force could be generated, reaching peak values of 7% body weight on average. Contrary to our expectation, the force mainly coincided with the propulsive phase of walking and not leg swing. Our findings suggest that subjects actively used their arms to harness the energy from the moving treadmill belt, which helped to propel the whole body via the arm-leg rope linkage. This effectively decreased the muscular and mechanical demands placed on the legs, reducing the propulsive impulse by 43% (p < 0.001), which led to a 17% net reduction in the metabolic power required for walking (p = 0.001).Conclusions: These findings provide the biomechanical and energetic basis for how we might reimagine the use of the arms in gait rehabilitation, opening the opportunity to explore if such a method could help patients regain their walking ability.Trial Registration: Study registered on 09/29/2018 in ClinicalTrials.gov (ID-NCT03689647). [ABSTRACT FROM AUTHOR]

To understand the principles of taste coding, it is necessary to understand the functional organization of the taste organs. Although the labellum of the Drosophila melanogaster head has been described in detail, the tarsal segments of the legs, which collectively contain more taste sensilla than the labellum, have received much less attention. We performed a systematic anatomical, physiological, and molecular analysis of the tarsal sensilla of Drosophila. We construct an anatomical map of all five tarsal segments of each female leg. The taste sensilla of the female foreleg are systematically tested with a panel of 40 diverse compounds, yielding a response matrix of ∼500 sensillum-tastant combinations. Six types of sensilla are characterized. One type was tuned remarkably broadly: it responded to 19 of 27 bitter compounds tested, as well as sugars; another type responded to neither. The midleg is similar but distinct from the foreleg. The response specificities of the tarsal sensilla differ from those of the labellum, as do n-dimensional taste spaces constructed for each organ, enhancing the capacity of the fly to encode and respond to gustatory information. We examined the expression patterns of all 68 gustatory receptors (Grs). A total of 28 Gr-GAL4 drivers are expressed in the legs. We constructed a receptor-to-sensillum map of the legs and a receptor-to-neuron map. Fourteen Gr-GAL4 drivers are expressed uniquely in the bitter-sensing neuron of the sensillum that is tuned exceptionally broadly. Integration of the molecular and physiological maps provides insight into the underlying basis of taste coding.

Context: Effective post-operative pain assessment is mandatory to exclude overdose of analgesics and avoid adverse effects. Patients in PACU have impaired ability to communicate making pain assessment challenging. This study aims to establish agreement between two pain scales, CPOT (Criticalcare Pain Observation Tool) and FLACC (Face, Legs, Activity, Cry, Consolability) and to find out specificity of combination of scales. Methods: Taking sample size of 50 patients of either sex, aged 18-80 years, ASA-PS I-III, undergoing elective surgeries were chosen, study period being June-September 2017. Adequately reversed, extubated patients not receiving sedatives, analgesics and local anaesthetics within 15 minutes before end of operation were included while patients with ASA-PS more than III or on ventilator were excluded. Assessment was done upto 2 hours at 30 minutes interval using CPOT tool and FLACC scale simultaneously by two observers, both being blinded about study. Results: Combination of two scales show high odds ratio (41%) and kappa coefficient (0.78) suggesting excellent agreement. Specificity of combination of scales is very high (95.2%) than individual test. Conclusion: CPOT and FLACC scale together has excellent agreement and their combination are more specific to assess the severity of post-operative pain than when used individually.

Understanding how sensorimotor cortex (SMC) organization relates to limb loss has major clinical implications, as cortical activity associated with phantom hand movements has been shown to predict phantom pain reports. Critically, earlier studies have largely focused on upper limb amputees; far less is known regarding SMC activity in lower limb amputees, despite the fact that this population comprises the majority of major limb loss cases. We aimed to characterize BOLD fMRI responses associated with phantom and sound limb movements to test the hypothesis that SMC organization is preserved in individuals with lower limb loss. Individuals with unilateral or bilateral lower limb loss underwent fMRI scans as they performed simple movements of their sound or phantom limbs. We observed that voluntary movements of the sound and phantom ankles were associated with BOLD signal changes in medial and superior portions of the precentral and postcentral gyri. In both hemispheres, contralateral limb movements were associated with greater signal changes compared to ipsilateral limb movements. Hand and mouth movements were associated with distinct activation patterns localized to more lateral SMC regions. We additionally tested whether activations associated with phantom movements related to self‐report assessments indexing phantom pain experiences, nonpainful phantom sensations and phantom movement capabilities. We found that responses during phantom ankle movements did not correlate with any of the composite phantom limb indices in our sample. Our collective results reveal that SMC representations of the amputated limb persist and that traditional somatotopic organization is generally preserved in individuals suffering from lower limb loss. [ABSTRACT FROM AUTHOR]

Painless legs and moving toes (PoLMT) is a rare variant of painful legs and moving toes (PLMT). The difference is the absence of pain, which is usually hard to manage in PLMT. An involuntary movement in the toes is a compound of both. Although there are many medication options for this disease, most fail to treat it successfully. Here, we report on a 62-year-old female with involuntary movements in her toes for five years. The rarity and unclear etiology of the disease are the reasons we wanted to present it. [ABSTRACT FROM AUTHOR]

Miniaturization strongly affects functional morphology. Whereas some anatomical structures are barely affected by scaling, others can fundamentally change as the body becomes ever smaller. No prior study has focused on the effect of miniaturization on grooming and attachment structures in Hymenoptera, which can be highly diverse and complex. Through comparative description of the legs of the extremely small wasps of the families Mymaridae and Trichogrammatidae, we evaluate the functional and phylogenetic patterns concerning possible functional effects of miniaturization. On the one hand, the studied species retain some features characteristic of other Chalcidoidea, while on the other, they display some parallelisms associated with miniaturization in leg structure. These observations support a two‐stage morphocline of miniaturization, wherein the first stage is characterized by the preservation of structural complexity and retention of all basic functions, as for instance in examined Megaphragma and the females of Dicopomorpha. The second stage is characterized by a significant simplification, with the loss of redundant non‐essential functions, as observed for the males of Dicopomorpha, which have grossly reduced leg structures, including total loss cleaning devices. Whether these stages are ordered or unordered should be evaluated in future study. Functional optimization of attachment in male Dicopomorpha is indicated by the highly derived mushroom‐shaped tarsi, complemented by novel grappling spurs on the hindfeet, possibly for copulation. Our observations underline adaptive trade‐offs in the expression of complex and multifunctional leg structures at extreme scales. [ABSTRACT FROM AUTHOR]

Background: Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods: To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results: Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions: The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases. [ABSTRACT FROM AUTHOR]