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This paper examines the impact of entrepreneurial initiatives within universities on scientific careers. Based on the career accounts of university-based bioscientists involved in a government-sponsored entrepreneurship training initiative, the paper explores the concept of academic entrepreneurialism. Three groups were identified in the data. First, academic entrepreneurs, who tended to be more experienced scientists and were now able to capitalise on their science. Second, those interested in technology transfer, who saw their career path taking them away from science, and finally a group of younger scientists who were trying to develop their career capital but were unsure what direction their career would take. The implications of these different groups for the management of universities and the development of knowledge are considered.

The nuclear enzyme poly(ADP-ribose) polymerase has been purified about 9200-fold from pig thymus nuclei with a 46% yield. An aqueous organic solvent system was used for the isolation of the polymerase from nuclei and for its purification by chromatography at sub-zero temperatures. Electrophoretic analysis under both denaturing and non-denaturing conditions revealed a single protein band suggesting that the preparation was homogeneous and that the enzyme is composed of one polypeptide chain. The molecular weight estimated from sodium dodecyl sulphate-/polyacrylamide gel electrophoresis was 63500 and from gel filtration through columns of Sephadex G-100, 58000. The enzyme preparation was free from poly(ADP-ribose)-degrading enzymes and from DNA. The purified polymerase showed an absolute requirement for both DNA and histones. The maximal specific activity of the homogeneous preparation measured by the standardized assay, was 20.7 μmol NAD+ incorporated × min-1 × mg-1 of protein at 37 °C. Amino-terminal group analysis with dansyl chloride did not reveal a terminal amino acid suggesting that the amino-terminal group may be blocked. In the presence of histones, the Km for NAD+ was 23 μM. [ABSTRACT FROM AUTHOR]