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173 results on '"Lee, Won-Jung"'

24. Central ANG II-receptor antagonists impair cardiovascular and vasopressin response to hemorrhage in rats

Author

Lee, Won-Jung, Yang, Eun-Ktoung, Ahn, Dong-kuk, Park, Yoon-Yub, Kim, Hyeong-Jin, and Park, Jae-Sik

Subjects
Hemorrhage -- Research, Angiotensin -- Physiological aspects, Biological sciences
Abstract

A study is conducted on conscious spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) and Wistar rats to determine the impairment of vasopressin and cardiovascular response to angiotensin II antagonists. The fall in blood pressure is higher during and after hemorrhage when losartan and saralasin are injected intracerebroventricularly in SHR and Wistar rats. Less vasopressin and more renin are released by intracerebroventricular angiotensin II antagonists after hemorrhage. No changes are observed in WKY rats.

Published
1995

38. DA-9601, a standardized extract of Artemisia asiatica, blocks TNF-α-induced IL-8 and CCL20 production by inhibiting p38 kinase and NF-κB pathways in human gastric epithelial cells

Author

Choi, Suck-Chei, Choi, Eun-Ju, Oh, Hyun-Mee, Lee, SungGa, Lee, Jeong-Kun, Lee, Meung-Su, Shin, Yong-Il, Choi, Suck-Jun, Chae, Jeong-Ryong, Lee, Kang-Min, Lee, Won-Jung, Park, Jae-Sik, Shin, Chang-Yell, Oh, Tae-Young, and Jun, Chang-Duk

Subjects
Chemokine CCL20, Transcription, Genetic, Plant Extracts, Tumor Necrosis Factor-alpha, Interleukin-8, JNK Mitogen-Activated Protein Kinases, NF-kappa B, Epithelial Cells, Macrophage Inflammatory Proteins, p38 Mitogen-Activated Protein Kinases, Basic Research, Artemisia, Gastric Mucosa, Genes, Reporter, Chemokines, CC, Humans, Enzyme Inhibitors, Extracellular Signal-Regulated MAP Kinases, Promoter Regions, Genetic, Signal Transduction
Abstract

To investigate whether, or how, DA-9601, which is a new gastroprotective agent, inhibits TNF-alpha-induced inflammatory signals in gastric epithelial AGS cells.Cell viability was determined by MTT assay. IL-8 and CCL20 promoter activities were determined by a luciferase reporter gene assay. NF-kappaB-dependent transcriptional activity was determined by I-kappaB alpha degradation, NF-kappaB p65 nuclear translocation and a luciferase activity assay. IL-8 and CCL20 gene expression and protein secretion were determined by RT-PCR and an enzyme-linked immunosorbent assay (ELISA). Total and phosphorylated forms of mitogen-activated protein kinases (MAPKs) were determined by Western blot.Treatment of AGS cells with DA-9601 reduced TNF-alpha-induced IL-8 and CCL20 promoter activities, as well as their gene expression and protein release. TNF-alpha also induced NF-kappaB-dependent transcriptional activity in AGS cells. In contrast, in cells treated with DA-9601, TNF-alpha-induced NF-kappaB activity was significantly blocked. Although all three MAP kinase family members were phosphorylated in response to TNF-alpha, a selective inhibitor of p38 kinase SB203580 only could inhibit both NF-kappaB-dependent transcriptional activity and IL-8 and CCL20 production, suggesting a potential link between p38 kinase and NF-kappaB-dependent pathways in AGS cells. Interestingly, DA-9601 also selectively inhibited p38 kinase phosphorylation induced by TNF-alpha.DA-9601 blocked TNF-alpha-mediated inflammatory signals by potentially modulating the p38 kinase pathway and/or a signal leading to NF-kappaB-dependent pathways in gastric epithelial cells.

Published
2006

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