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10 results on '"Lee, Shi Mun"'

1. Life stage impact on the human skin ecosystem: lipids and the microbial community.

Author

Pagac, Martin P., Davient, Bala, Plado, Luca Antonio, Lam, Hilbert Yuen In, Lee, Shi Mun, Ravikrishnan, Aarthi, Chua, Wee Ling Esther, Muralidharan, Sneha, Sridharan, Aishwarya, Irudayaswamy, Antony S., Srinivas, Ramasamy, Wearne, Stephen, Mohamed Naim, Ahmad Nazri, Ho, Eliza Xin Pei, Ng, H. Q. Amanda, Kwah, Junmei Samantha, Png, Eileen, Bendt, Anne K., Wenk, Markus R., and Torta, Federico

Published
2025
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2. Systematic evaluation of CRISPR-Cas systems reveals design principles for genome editing in human cells

Author

Wang, Yuanming, Liu, Kaiwen Ivy, Sutrisnoh, Norfala-Aliah Binte, Srinivasan, Harini, Zhang, Junyi, Li, Jia, Zhang, Fan, Lalith, Charles Richard John, Xing, Heyun, Shanmugam, Raghuvaran, Foo, Jia Nee, Yeo, Hwee Ting, Ooi, Kean Hean, Bleckwehl, Tore, Par, Yi Yun Rachel, Lee, Shi Mun, Ismail, Nur Nadiah Binte, Sanwari, Nur Aidah Binti, Lee, Si Ting Vanessa, Lew, Jan, and Tan, Meng How

Published
2018
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8. Effect of zinc pyrithione shampoo treatment on skin commensal Malassezia.

Author

Leong, Cheryl, Wang, Joyce, Toi, Min Jet, Lam, Yuen In, Goh, Joleen PZ, Lee, Shi Mun, and Dawson, Thomas L

Abstract

Malassezia restricta and Malassezia globosa are lipid dependent commensal yeasts associated with dandruff. Antifungal actives such as zinc pyrithione are commonly used in antidandruff shampoos, although their efficacy is not clearly demonstrated. In this study, we assessed the efficacy of antifungal treatments on scalp Malassezia via a combination of culturomic and genomic detection methods. Zinc pyrithione inhibited Malassezia growth at low minimum inhibitory concentrations (MICs). In a longitudinal pilot study, quantitative polymerase chain reaction (qPCR) analysis showed a decrease in M. restricta on the scalp after zinc pyrithione treatment. These findings validate the antifungal efficacy of zinc pyrithione as a dandruff treatment. Lay Abstract Malassezia yeasts are associated with dandruff and seborrheic dermatitis. Zinc pyrithione is effective against Malassezia growth in vitro and when tested on human skin as a shampoo. These findings will be useful for investigating the role of Malassezia in skin microbiome intervention studies. [ABSTRACT FROM AUTHOR]

Published
2021
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9. Optimizing directed evolution of S. pyogenes Cas9

Author

Lee, Shi Mun, Tan Meng How, and School of Chemical and Biomedical Engineering

Subjects
Engineering::Bioengineering [DRNTU]
Abstract

CRISPR/Cas9, since its discovery, has revolutionized the genome editing landscape; with one protein and a programmable RNA sequence, genome targeting has become achievable even in the non-experts’ hands. Despite its easy adaptation of the application, off-target and low efficiency have been issues to push its use in genetic therapy. In this research, attempts to enhance the efficiency of nuclease activity were attempted. Random mutagenesis has been performed in the region of the nuclease domain – RuvCII-HNH-RuvCIII. The enhanced mutants were expected to be isolated from a positive selection system where the survival of the host is linked to the Cas9 ability to severe the suicide plasmid. However, problems had arisen due to high background and toxicity issues; and attempts to optimize the condition to eliminate the problem has become the goal of this project. ​Master of Science (Biomedical Engineering)

Published
2017

10. A Novel Virus Alters Gene Expression and Vacuolar Morphology in MalasseziaCells and Induces a TLR3-Mediated Inflammatory Immune Response

Author

Park, Minji, Cho, Yong-Joon, Kim, Donggyu, Yang, Chul-Su, Lee, Shi Mun, Dawson, Thomas L., Nakamizo, Satoshi, Kabashima, Kenji, Lee, Yang Won, and Jung, Won Hee

Abstract

Malasseziais the most dominant fungal genus on the human skin surface and is associated with various skin diseases including dandruff and seborrheic dermatitis. Among Malasseziaspecies, Malassezia restrictais the most widely observed species on the human skin. In the current study, we identified a novel dsRNA virus, named MrV40, in M. restrictaand characterized the sequence and structure of the viral genome along with an independent satellite dsRNA viral segment. Moreover, expression of genes involved in ribosomal synthesis and programmed cell death was altered, indicating that virus infection affected the physiology of the fungal host cells. Our data also showed that the viral nucleic acid from MrV40 induces a TLR3-mediated inflammatory immune response in bone marrow-derived dendritic cells, indicating that a viral element likely contributes to the pathogenicity of Malassezia. This is the first study to identify and characterize a novel mycovirus in Malassezia.

Published
2020
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