9 results on '"Lee, Huseong"'
Search Results
2. Effect of residual methane emission on physiological characteristics and carcass performance in Japanese Black cattle
- Author
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Kim, Minji, primary, Masaki, Tatsunori, additional, Oikawa, Kohei, additional, Ashihara, Akane, additional, Ikuta, Kentaro, additional, Iwamoto, Eiji, additional, Lee, Huseong, additional, Haga, Satoshi, additional, Uemoto, Yoshinobu, additional, Roh, Sanggun, additional, Terada, Fuminori, additional, and Nonaka, Itoko, additional
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- 2024
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3. PSII-8 Comparison of Different Fattening Stages on Rumen Microbiota and Physiological Characteristics in Japanese Black Cattle
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Lee, Huseong, primary, Kim, Minji, additional, Masaki, Tatsunori, additional, Ikuta, Kentaro, additional, Iwamoto, Eiji, additional, Nishihara, Koki, additional, Hirai, Makoto, additional, Uemoto, Yoshinobu, additional, Terada, Fuminori, additional, and Roh, Sanggun, additional
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- 2023
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4. Characteristics of Physiological Parameters of Japanese Black Calves Relate to Carcass Weight
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Arakawa, Shotaro, primary, Kim, Minji, additional, Aonuma, Tatsuya, additional, Takagi, Michihiro, additional, Watanabe, Satoshi, additional, Lee, Huseong, additional, Nishihara, Koki, additional, Haga, Satoshi, additional, Uemoto, Yoshinobu, additional, and Roh, Sanggun, additional
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- 2023
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5. The impact of different diets and genders on fecal microbiota in Hanwoo cattle
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Sim, Seunghyeun, primary, Lee, Huseong, additional, Yoon, Sang, additional, Seon, Hyeonsu, additional, Park, Cheolju, additional, and Kim, Minseok, additional
- Published
- 2022
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6. Physiological roles and regulation of hepatic angiopoietin-like protein 3 in Japanese Black cattle (Bos taurus)during the fattening period
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Shikida, Rika, Kim, Minji, Futohashi, Makoto, Nishihara, Koki, Lee, Huseong, Suzuki, Yutaka, Baek, Yeolchang, Masaki, Tatsunori, Ikuta, Kentaro, Iwamoto, Eiji, Uemoto, Yoshinobu, Haga, Satoshi, Terada, Fuminori, and Roh, Sanggun
- Abstract
Angiopoietin-like protein 3 (ANGPTL3) is expressed predominantly in the liver and plays a major role in regulating the circulating triglyceride and lipoprotein fraction concentrations by inhibiting lipoprotein lipase (LPL) activity. Given these physiological roles, ANGPTL3 may play an important role in metabolic changes related to fat accumulation during the fattening period in Japanese Black. This study aimed to reveal the physiological roles of hepatic ANGPTL3 in Japanese Black steers (Bos taurus) during the fattening period and investigate the regulatory effects of hepatic ANGPTL3. To investigate the gene expression and protein localization of ANGPTL3, 18 tissue samples were collected from tree male Holstein bull calves aged 7 wk. Biopsied liver tissues and blood samples were collected from 21 Japanese Black steers during the early (T1; 13 mo of age), middle (T2; 20 mo), and late fattening phases (T3; 28 mo). Relative mRNA expression, blood metabolite concentrations, hormone concentrations, growth, and carcass traits were analyzed. To identify the regulatory factors of hepatic ANGPTL3, primary bovine hepatocytes collected by two Holstein calves aged 7 wk were incubated with insulin, palmitate, oleate, propionate, acetate, or beta-hydroxybutyric acid (BHBA). The ANGPTL3gene was most highly expressed in the liver, with minor expression in the renal cortex, lungs, reticulum, and jejunum in Holstein bull calves. In Japanese Black steers, relative ANGPTL3mRNA expressions were less as fattening progressed, and blood triglyceride, total cholesterol, and nonesterified fatty acid (NEFA) concentrations increased. Relative ANGPTL8and Liver X receptor alpha (LXRα)mRNA expressions decreased in late and middle fattening phases, respectively. Furthermore, relative ANGTPL3mRNA expression was positively correlated with ANGPTL8(r= 0.650; P< 0.01) and ANGPTL4(r= 0.540; P< 0.05) in T3 and T1, respectively, and LXRαshowed no correlation with ANGPTL3. Relative ANGTPL3mRNA expression was negatively correlated with total cholesterol (r= −0.434; P< 0.05) and triglyceride (r= −0.645; P< 0.01) concentrations in T3 and T1, respectively; There was no significant correlation between ANGTPL3and carcass traits. Relative ANGTPL3mRNA expression in cultured bovine hepatocytes was downregulated in oleate treatment. Together, these findings suggest that ANGPTL3downregulation in late fattening phases is associated with the changes in lipid metabolism.Physiological roles of angiopoietin-like protein 3 (ANGPTL3) are involved with the lipid metabolism in fattening Japanese Black steers, and the relative mRNA expression of genes in the ANGPTLfamily and blood lipid metabolites seem to be associated with each other.The role of angiopoietin-like protein 3 (ANGPTL3) in various animal species under different physiological conditions remains largely unknown. We evaluated the physiological roles of hepatic ANGPTL3 in Japanese Black steers (Bos taurus) during the fattening period and investigated the expressional regulation of ANGPTL3in bovine hepatocytes. Relative ANGPTL3mRNA expression decreased late in the fattening phases. Relative ANGPTL3mRNA expression was positively correlated with ANGPTL4and ANGPTL8and was negatively correlated with blood triglyceride concentrations in early fattening phases. Relative ANGPTL3mRNA expression in cultured bovine hepatocytes was downregulated in oleate treatment. Fatty acids may influence ANGPTL3expression in cultured bovine hepatocytes through possible regulatory factors. Our findings suggest that the physiological roles of ANGPTL3 are associated with the changes of lipid metabolism during the fattening period, and the ANGPTL family seem to be associated with blood lipid metabolites.
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- 2023
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7. Dynamics of Blood Taurine Concentration and its Correlation with Nutritional and Physiological Status during the Fattening Period of Japanese Black Cattle.
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Takai S, Lee H, Kim M, Torii S, Koki N, Oh J, Masaki T, Ikuta K, Iwamoto E, Masuda K, Uemoto Y, Terada F, Haga S, and Roh S
- Abstract
Taurine, biosynthesized from methionine or cysteine in the liver, plays a crucial regulatory role in bile acid conjugation, antioxidant effects, and glucose and cholesterol metabolism. This may influence the metabolic changes associated with fat accumulation in beef cattle. However, the physiological role of taurine in this species has not been fully elucidated. In this study, we explored the physiological role of taurine in Japanese Black steers (Bos taurus) in different phases during the fattening period. To examine the correlation among plasma taurine concentrations, various physiological parameters, and genes related to taurine synthesis in the liver, we used biopsied liver tissues, blood samples, and rumen fluids collected from 21 steers at three different stages, i.e., early (T1; 13 months of age), middle (T2; 20 months of age), and late (T3; 28 months of age) phases. Additionally, to investigate the regulatory mechanisms underlying the expression profile of taurine synthesis genes, primary bovine hepatocytes obtained from 4-week-old Holstein calves were treated with palmitate, oleate, acetate, propionate, or β-hydroxybutyrate (BHBA). Plasma taurine and cholesterol concentrations significantly (P < 0.001) increased in the T2 phase, which is potentially attributable to increased energy intake and assimilation induced by increased intake of concentrated feed. Cysteine sulfinic acid decarboxylase (CSAD) expression significantly increased (P < 0.01) in T2 than in other phases. The expression levels of cysteine dioxygenase type 1 (CDO1) and cholesterol 7 alpha-hydroxylase (CYP7A1) were significantly higher (P < 0.001) in T2 than in T3; moreover, the CDO1/glutamate-cysteine ligase catalytic subunit (GCLC) ratio was higher (P < 0.05) in T2 than in T1. Plasma taurine concentrations were positively correlated with plasma methionine (r = 0.51; P < 0.05) and total cholesterol (r = 0.56; P < 0.05) concentrations at T2. Relative CDO1 mRNA expression was upregulated in cultured bovine hepatocytes treated with oleate and propionate, whereas it was downregulated upon acetate treatment. These findings indicate that the increase in plasma taurine concentrations in the T2 phase is associated with changes in lipid and methionine metabolism in Japanese Black steers., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.)
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- 2024
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8. Effects of diets for three growing stages by rumen inocula donors on in vitro rumen fermentation and microbiome.
- Author
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Kang R, Lee H, Seon H, Park C, Song J, Park JK, Kim YK, Kim M, and Park T
- Abstract
Hanwoo and Jeju Black cattle (Jeju Black) are native breeds of Korean cattle. Jeju Black cattle are recognized as natural monuments and are known to exhibit slower growth rates compared to Hanwoo. While several studies have analyzed the genetic characteristics of these cattle, there has been limited research on the differences in their microbiome. In this study, rumen fluid was obtained from three Hanwoo steers and three Jeju Black steers, and three different diets (total mixed rations [TMRs] for growing, early fattening, and late fattening periods) were used as substrates for in vitro fermentation. The in vitro incubation was conducted for 3 h and 24 h following a 2 × 3 factorial arrangement. After both incubation periods, fermentation characteristics were analyzed, and ruminal microbiome analysis was performed using 16S rRNA gene sequencing, employing both QIIME2 and PICRUSt2. The results revealed significant differences in the ruminal microbiota due to the inoculum effect. At the phylum level, Patescibacteria and Synergistota were found to be enriched in the Jeju Black inoculum-treated group. Additionally, using different inocula also affected the relative abundance of major taxa, including Ruminococcus, Pseudoramibacter, Ruminococcaceae CAG-352, and the [ Eubacterium ] ruminantium group. These microbial differences induced by the inoculum may have originated from varying levels of domestication between the two subspecies of donor animals, which mainly influenced the fermentation and microbiome features in the early incubation stages, although this was only partially offset afterward. Furthermore, predicted commission numbers of microbial enzymes, some of which are involved in the biosynthesis of secondary metabolites, fatty acids, and alpha amylase, differed based on the inoculum effect. However, these differences may account for only a small proportion of the overall metabolic pathway. Conversely, diets were found to affect protein biosynthesis and its related metabolism, which showed differential abundance in the growing diet and were potentially linked to the growth-promoting effects in beef cattle during the growing period. In conclusion, this study demonstrated that using different inocula significantly affected in vitro fermentation characteristics and microbiome features, mainly in the early stages of incubation, with some effects persisting up to 24 h of incubation., Competing Interests: No potential conflict of interest relevant to this article was reported., (© Copyright 2024 Korean Society of Animal Science and Technology.)
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- 2024
- Full Text
- View/download PDF
9. Physiological roles and regulation of hepatic angiopoietin-like protein 3 in Japanese Black cattle (Bos taurus) during the fattening period.
- Author
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Shikida R, Kim M, Futohashi M, Nishihara K, Lee H, Suzuki Y, Baek Y, Masaki T, Ikuta K, Iwamoto E, Uemoto Y, Haga S, Terada F, and Roh S
- Subjects
- Animals, Cattle, Male, Angiopoietin-like Proteins genetics, Angiopoietin-like Proteins metabolism, Cholesterol, Liver metabolism, RNA, Messenger metabolism, Triglycerides metabolism, Angiopoietin-Like Protein 3, Oleic Acid
- Abstract
Angiopoietin-like protein 3 (ANGPTL3) is expressed predominantly in the liver and plays a major role in regulating the circulating triglyceride and lipoprotein fraction concentrations by inhibiting lipoprotein lipase (LPL) activity. Given these physiological roles, ANGPTL3 may play an important role in metabolic changes related to fat accumulation during the fattening period in Japanese Black. This study aimed to reveal the physiological roles of hepatic ANGPTL3 in Japanese Black steers (Bos taurus) during the fattening period and investigate the regulatory effects of hepatic ANGPTL3. To investigate the gene expression and protein localization of ANGPTL3, 18 tissue samples were collected from tree male Holstein bull calves aged 7 wk. Biopsied liver tissues and blood samples were collected from 21 Japanese Black steers during the early (T1; 13 mo of age), middle (T2; 20 mo), and late fattening phases (T3; 28 mo). Relative mRNA expression, blood metabolite concentrations, hormone concentrations, growth, and carcass traits were analyzed. To identify the regulatory factors of hepatic ANGPTL3, primary bovine hepatocytes collected by two Holstein calves aged 7 wk were incubated with insulin, palmitate, oleate, propionate, acetate, or beta-hydroxybutyric acid (BHBA). The ANGPTL3 gene was most highly expressed in the liver, with minor expression in the renal cortex, lungs, reticulum, and jejunum in Holstein bull calves. In Japanese Black steers, relative ANGPTL3 mRNA expressions were less as fattening progressed, and blood triglyceride, total cholesterol, and nonesterified fatty acid (NEFA) concentrations increased. Relative ANGPTL8 and Liver X receptor alpha (LXRα) mRNA expressions decreased in late and middle fattening phases, respectively. Furthermore, relative ANGTPL3 mRNA expression was positively correlated with ANGPTL8 (r = 0.650; P < 0.01) and ANGPTL4 (r = 0.540; P < 0.05) in T3 and T1, respectively, and LXRα showed no correlation with ANGPTL3. Relative ANGTPL3 mRNA expression was negatively correlated with total cholesterol (r = -0.434; P < 0.05) and triglyceride (r = -0.645; P < 0.01) concentrations in T3 and T1, respectively; There was no significant correlation between ANGTPL3 and carcass traits. Relative ANGTPL3 mRNA expression in cultured bovine hepatocytes was downregulated in oleate treatment. Together, these findings suggest that ANGPTL3 downregulation in late fattening phases is associated with the changes in lipid metabolism., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2023
- Full Text
- View/download PDF
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