1. Chaperone-like protein DAY plays critical roles in photomorphogenesis
- Author
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Sung Hee Lee, Hee-Kyung Ahn, Tak Lee, Insuk Lee, Hyun Sook Pai, Ilyeong Choi, Dong Hye Seo, Ji Woo Kim, Ho Seok Lee, Huikyong Cho, Jae Hee Kim, Julian Bünting, Young Eun Jeon, Youssef Belkhadir, D. Lee, Tae Wuk Kim, Jung Min Lee, Man Ho Oh, Ahn, Hee-Kyung [0000-0002-8884-0156], Lee, Tak [0000-0001-7008-7605], Lee, Du-Hwa [0000-0003-4106-7730], Lee, Insuk [0000-0003-3146-6180], Belkhadir, Youssef [0000-0001-7149-7139], Pai, Hyun-Sook [0000-0003-1345-5840], Apollo - University of Cambridge Repository, Gregor Mendel Institute of Molecular Plant Biology (GMI), Austrian Academy of Sciences (OeAW), Yonsei University Seoul, Korea, University of East Anglia [Norwich] (UEA), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Sainsbury Laboratory Cambridge University (SLCU), University of Cambridge [UK] (CAM), College of Life Sciences and Biotechnology, Korea University, Chungnam National University (CNU), Hanyang University, Vienna Biocenter - VBC [Austria], and Yonsei University
- Subjects
0106 biological sciences ,0301 basic medicine ,Chlorophyll ,Chloroplasts ,Light ,[SDV]Life Sciences [q-bio] ,Arabidopsis ,General Physics and Astronomy ,631/449/2653/1359 ,01 natural sciences ,chemistry.chemical_compound ,38/1 ,Gene Expression Regulation, Plant ,Morphogenesis ,Brassinosteroid ,RNA-Seq ,14/19 ,13/89 ,Multidisciplinary ,biology ,Chemistry ,article ,Plant morphogenesis ,Plant physiology ,food and beverages ,Plants, Genetically Modified ,Recombinant Proteins ,Cell biology ,Chloroplast ,Transmembrane domain ,Gene Knockdown Techniques ,Photomorphogenesis ,38/39 ,Microtubule-Associated Proteins ,Signal Transduction ,Science ,38/90 ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Brassinosteroids ,Etiolation ,Light responses ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Endomembrane system ,14/35 ,631/449/2686 ,Arabidopsis Proteins ,fungi ,Membrane Proteins ,General Chemistry ,HSP40 Heat-Shock Proteins ,030104 developmental biology ,Seedlings ,Chaperone (protein) ,Mutation ,14/63 ,biology.protein ,Protein Kinases ,Biogenesis ,010606 plant biology & botany - Abstract
Photomorphogenesis, light-mediated development, is an essential feature of all terrestrial plants. While chloroplast development and brassinosteroid (BR) signaling are known players in photomorphogenesis, proteins that regulate both pathways have yet to be identified. Here we report that DE-ETIOLATION IN THE DARK AND YELLOWING IN THE LIGHT (DAY), a membrane protein containing DnaJ-like domain, plays a dual-role in photomorphogenesis by stabilizing the BR receptor, BRI1, as well as a key enzyme in chlorophyll biosynthesis, POR. DAY localizes to both the endomembrane and chloroplasts via its first transmembrane domain and chloroplast transit peptide, respectively, and interacts with BRI1 and POR in their respective subcellular compartments. Using genetic analysis, we show that DAY acts independently on BR signaling and chlorophyll biogenesis. Collectively, this work uncovers DAY as a factor that simultaneously regulates BR signaling and chloroplast development, revealing a key regulator of photomorphogenesis that acts across cell compartments., Chloroplast development and brassinosteroid (BR) signaling both regulate plant photomorphogenesis. Here the authors show that DAY, a DnaJ-like domain-containing membrane protein, regulates both processes by binding and stabilizing both BRI1, the BR receptor, and POR, a key enzyme in chlorophyll biosynthesis.
- Published
- 2021
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