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2. Central nervous system infection in a pediatric population in West Java

Author

Alisjahbana, Dewi H., primary, Nurmawati, Syndi, additional, Milanti, Mia, additional, Djauhari, Hofiya, additional, Ledermann, Jeremy P., additional, Antonjaya, Ungke, additional, Dewi, Yora Permata, additional, Johar, Edison, additional, Wiyatno, Ageng, additional, Sriyani, Ida Yus, additional, Alisjahbana, Bachti, additional, Safari, Dodi, additional, Myint, Khin Saw Aye, additional, Powers, Ann M., additional, and Hakim, Dzulfikar DL, additional

Published
2023
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4. Improved Mosquito Housing and Saliva Collection Method Enhances Safety While Facilitating Longitudinal Assessment of Individual Mosquito Vector Competence for Arboviruses.

Author

Ledermann, Jeremy P., Burns, Paul L., Perinet, Lara C., Powers, Ann M., and Byers, Nathaniel M.

Subjects
*MOSQUITO vectors, *MOSQUITOES, *SALIVA, *WEST Nile virus, *ARBOVIRUSES, *MOSQUITO control, *TRADITIONAL farming
Abstract

Background: Assessing the potential for mosquitoes to transmit medically important arboviruses is essential for understanding their threat to human populations. Currently, vector competence studies are typically performed by collecting saliva using a glass capillary tube system which involves sacrificing the mosquito at the time of saliva collection allowing only a single data point. These techniques also require handling infected mosquitoes and glass capillaries, constituting a safety risk. Materials and Methods: To improve the efficiency and safety of assessing vector competence, a novel containment and saliva collection approach for individually housed mosquitoes was developed. The improved housing, allowing longitudinal tracking of individual mosquitoes, consists of a 12-well Corning polystyrene plate sealed with a three-dimensional printed lid that holds organdy netting firmly against the rims of the wells. Results: This method provides excellent mosquito survival for five species of mosquitoes, with at least 79% of each species tested surviving for more than 2 weeks, comparable to the carton survival rates of ≥76%. When the plate housing system was used to assess vector infection, replication of West Nile virus (WNV) in mosquito tissues was similar to traditional containment mosquito housing. Mosquito saliva was collected using either blotting paper pads or traditional glass capillaries to assay viral transmission. The blotting paper collection showed similar or better sensitivity than the capillary method; in addition, longitudinal saliva samples could be collected from individual mosquitoes housed in the 12-well plates. Conclusions: The improved housing and saliva collection technique described herein provides a safer and more informative method for determining vector competence in mosquitoes. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Exposure of Egyptian Rousette Bats (Rousettus aegyptiacus) and a Little Free-Tailed Bat (Chaerephon pumilus) to Alphaviruses in Uganda

Author

Kading, Rebekah C., primary, Borland, Erin M., additional, Mossel, Eric C., additional, Nakayiki, Teddy, additional, Nalikka, Betty, additional, Ledermann, Jeremy P., additional, Crabtree, Mary B., additional, Panella, Nicholas A., additional, Nyakarahuka, Luke, additional, Gilbert, Amy T., additional, Kerbis-Peterhans, Julian C., additional, Towner, Jonathan S., additional, Amman, Brian R., additional, Sealy, Tara K., additional, Miller, Barry R., additional, Lutwama, Julius J., additional, Kityo, Robert M., additional, and Powers, Ann M., additional

Published
2022
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8. Neurological Disease Associated with Chikungunya in Indonesia

Author

Myint, Khin S. A., primary, Mawuntu, Arthur H. P., additional, Haryanto, Sotianingsih, additional, Imran, Darma, additional, Dian, Sofiati, additional, Dewi, Yora P., additional, Ganiem, Ahmad R., additional, Anggreani, Riane, additional, Iskandar, Mirna M., additional, Bernadus, Janno B. B., additional, Maharani, Kartika, additional, Susanto, David, additional, Estiasari, Riwanti, additional, Dewi, Hasna, additional, Kristiani, Amanda, additional, Gaghiwu, Lidia, additional, Johar, Edison, additional, Yudhaputri, Frilasita A., additional, Antonjaya, Ungke, additional, Ledermann, Jeremy P., additional, van Crevel, Reinout, additional, Hamers, Raph L., additional, and Powers, Ann M., additional

Published
2022
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12. Japanese encephalitis virus infection in non-encephalitic acute febrile illness patients

Author

Ma’roef, Chairin Nisa, primary, Dhenni, Rama, additional, Megawati, Dewi, additional, Fadhilah, Araniy, additional, Lucanus, Anton, additional, Artika, I Made, additional, Masyeni, Sri, additional, Lestarini, Asri, additional, Sari, Kartika, additional, Suryana, Ketut, additional, Yudhaputri, Frilasita A., additional, Jaya, Ungke Anton, additional, Sasmono, R. Tedjo, additional, Ledermann, Jeremy P., additional, Powers, Ann M., additional, and Myint, Khin Saw Aye, additional

Published
2020
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13. Isolation of Zika virus from febrile patient, Indonesia

Author

Perkasa, Aditya, Yudhaputri, Frilasita, Haryanto, Sotianingsih, Hayati, Rahma F., Maroef, Chairin Nisa, Antonjaya, Ungke, Yohan, Benediktus, Myint, Khin Saw Aye, Ledermann, Jeremy P., Rosenberg, Ronald, Powers, Ann M., and Sasmono, R. Tedjo

Subjects
Health
Abstract

To the Editor: Arthropodborne viruses (arboviruses) cause substantial human disease worldwide and have a pronounced effect on public health throughout Asia. Zika virus, discovered in Uganda in 1947 (1), is [...]

Published
2016

14. Japanese encephalitis virus infection in non-encephalitic acute febrile illness patients.

Author

Ma'roef, Chairin Nisa, Dhenni, Rama, Megawati, Dewi, Fadhilah, Araniy, Lucanus, Anton, Artika, I Made, Masyeni, Sri, Lestarini, Asri, Sari, Kartika, Suryana, Ketut, Yudhaputri, Frilasita A., Jaya, Ungke Anton, Sasmono, R. Tedjo, Ledermann, Jeremy P., Powers, Ann M., and Myint, Khin Saw Aye

Subjects
JAPANESE encephalitis viruses, VIRUS diseases, ACUTE diseases, SERODIAGNOSIS, DENGUE viruses, NEUROCYSTICERCOSIS, DENGUE hemorrhagic fever
Abstract

Although Japanese encephalitis virus (JEV) is considered endemic in Indonesia, there are only limited reports of JEV infection from a small number of geographic areas within the country with the majority of these being neuroinvasive disease cases. Here, we report cases of JEV infection in non-encephalitic acute febrile illness patients from Bali, Indonesia. Paired admission (S1) and discharge (S2) serum specimens from 144 acute febrile illness patients (without evidence of acute dengue virus infection) were retrospectively tested for anti-JEV IgM antibody and confirmed by plaque reduction neutralization test (PRNT) for JEV infection. Twenty-six (18.1%) patients were anti-JEV IgM-positive or equivocal in their S2 specimens, of which 5 (3.5%) and 8 (5.6%) patients met the criteria for confirmed and probable JEV infection, respectively, based on PRNT results. Notably, these non-encephalitic JE cases were less likely to have thrombocytopenia, leukopenia, and lower hematocrit compared with confirmed dengue cases of the same cohort. These findings highlight the need to consider JEV in the diagnostic algorithm for acute febrile illnesses in endemic areas and suggest that JEV as a cause of non-encephalitic disease has likely been underestimated in Indonesia. Author summary: Japanese encephalitis virus (JEV) is an important cause of central nervous system (CNS) infections in Asia and is considered endemic in Indonesia. However, reports of JEV infection in non-encephalitic disease cases are lacking because diagnosis is difficult to confirm and JEV is rarely considered as a cause of non-encephalitic disease. Here, with robust serological testing, we identified cases of JEV infection in patients presenting at a regency hospital in Bali with fever but without symptoms of CNS infection. This finding supports the need to include JEV in routine clinical diagnostic algorithms for patients with fever in endemic areas. [ABSTRACT FROM AUTHOR]

Published
2020
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15. Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient

Author

Dhenni, Rama, primary, Karyanti, Mulya Rahma, additional, Putri, Nina Dwi, additional, Yohan, Benediktus, additional, Yudhaputri, Frilasita A., additional, Ma'roef, Chairin Nisa, additional, Fadhilah, Araniy, additional, Perkasa, Aditya, additional, Restuadi, Restuadi, additional, Trimarsanto, Hidayat, additional, Mangunatmadja, Irawan, additional, Ledermann, Jeremy P., additional, Rosenberg, Ronald, additional, Powers, Ann M., additional, Myint, Khin Saw Aye, additional, and Sasmono, R. Tedjo, additional

Published
2018
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16. Neutralizing antibodies against flaviviruses, Babanki virus, and Rift Valley fever virus in Ugandan bats

Author

Kading, Rebekah C., primary, Kityo, Robert M., additional, Mossel, Eric C., additional, Borland, Erin M., additional, Nakayiki, Teddie, additional, Nalikka, Betty, additional, Nyakarahuka, Luke, additional, Ledermann, Jeremy P., additional, Panella, Nicholas A., additional, Gilbert, Amy T., additional, Crabtree, Mary B., additional, Peterhans, Julian Kerbis, additional, Towner, Jonathan S., additional, Amman, Brian R., additional, Sealy, Tara K., additional, Nichol, Stuart T., additional, Powers, Ann M., additional, Lutwama, Julius J., additional, and Miller, Barry R., additional

Published
2018
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18. Genomic characterization of Zika virus isolated from Indonesia

Author

Yudhaputri, Frilasita A., primary, Trimarsanto, Hidayat, additional, Perkasa, Aditya, additional, Yohan, Benediktus, additional, Haryanto, Sotianingsih, additional, Wiyatno, Ageng, additional, Soebandrio, Amin, additional, Myint, Khin Saw, additional, Ledermann, Jeremy P., additional, Rosenberg, Ronald, additional, Powers, Ann M., additional, and Sasmono, R. Tedjo, additional

Published
2017
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19. Increased rates of Guillain-Barré syndrome associated with Zika virus outbreak in the Salvador metropolitan area, Brazil

Author

Styczynski, Ashley R., primary, Malta, Juliane M. A. S., additional, Krow-Lucal, Elisabeth R., additional, Percio, Jadher, additional, Nóbrega, Martha E., additional, Vargas, Alexander, additional, Lanzieri, Tatiana M., additional, Leite, Priscila L., additional, Staples, J. Erin, additional, Fischer, Marc X., additional, Powers, Ann M., additional, Chang, Gwong-Jen J., additional, Burns, P. L., additional, Borland, Erin M., additional, Ledermann, Jeremy P., additional, Mossel, Eric C., additional, Schonberger, Lawrence B., additional, Belay, Ermias B., additional, Salinas, Jorge L., additional, Badaro, Roberto D., additional, Sejvar, James J., additional, and Coelho, Giovanini E., additional

Published
2017
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21. Detection of central nervous system viral infections in adults in Manado, North Sulawesi, Indonesia.

Author

Mawuntu, Arthur H. P., Bernadus, Janno B. B., Dhenni, Rama, Wiyatno, Ageng, Anggreani, Riane, Feliana, null, Yudhaputri, Frilasita A., Jaya, Ungke Anton, Ma’roef, Chairin Nisa, Dewantari, Aghnianditya K., Fadhilah, Araniy, Ledermann, Jeremy P., Powers, Ann M., Safari, Dodi, and Myint, Khin Saw Aye

Subjects
DIAGNOSIS of central nervous system diseases, VIRAL disease diagnosis, MORTALITY, HOSPITAL admission & discharge
Abstract

Central nervous system (CNS) viral infections are important causes of morbidity and mortality worldwide but the systematic survey of patients admitted to hospitals with CNS infections in many countries, including Indonesia, is limited. To obtain more information regarding the causes of CNS infections in Indonesia, this study was performed to detect and identify viral agents associated with CNS infections amongst in-patients at a referral hospital in Manado, North Sulawesi, Indonesia. Adult patients admitted to R.D. Kandou General Hospital with presumed CNS infection were enrolled. Cerebrospinal fluid, serum, and throat swab samples were collected and tested using molecular, serological, and virus isolation assays. A confirmed viral etiology was established in three and a probable/possible in 11 out of 74 patients. The most common was herpes simplex virus 1 (7/74, 9.5%), followed by Epstein-Barr virus (2/74, 2.7%), cytomegalovirus (1/74, 1.4%), enterovirus D68 (1/74, 1.4%), rhinovirus A (1/74, 1.4%), dengue virus (1/64, 1.6%), and Japanese encephalitis virus (1/64, 1.6%). There were 20 fatal cases (27.0%) during hospitalization in which eight were associated with viral causes. We identified herpes simplex virus 1 as the most common cause of CNS infection among adults in North Sulawesi with most of the cases remaining undiagnosed. Our study highlights the challenges in establishing the etiology of viral CNS infections and the importance of using a wide range of molecular and serological detection methods to identify CNS viruses. [ABSTRACT FROM AUTHOR]

Published
2018
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22. Aedes hensilli as a Potential Vector of Chikungunya and Zika Viruses

Author

Guillaumot, Laurent and Ledermann, Jeremy P.

Abstract

An epidemic of Zika virus (ZIKV) illness that occurred in July 2007 on Yap Island in the Federated States of Micronesia prompted entomological studies to identify both the primary vector(s) involved in transmission and the ecological parameters contributing to the outbreak. Larval and pupal surveys were performed to identify the major containers serving as oviposition habitat for the likely vector(s). Adult mosquitoes were also collected by backpack aspiration, light trap, and gravid traps at select sites around the capital city. The predominant species found on the island was Aedes (Stegomyia) hensilli. No virus isolates were obtained from the adult field material collected, nor did any of the immature mosquitoes that were allowed to emerge to adulthood contain viable virus or nucleic acid. Therefore, laboratory studies of the probable vector, Ae. hensilli, were undertaken to determine the likelihood of this species serving as a vector for Zika virus and other arboviruses. Infection rates of up to 86%, 62%, and 20% and dissemination rates of 23%, 80%, and 17% for Zika, chikungunya, and dengue-2 viruses respectively, were found supporting the possibility that this species served as a vector during the Zika outbreak and that it could play a role in transmitting other medically important arboviruses.

Published
2014

26. Aedes hensilli as a Potential Vector of Chikungunya and Zika Viruses

Author

Ledermann, Jeremy P., primary, Guillaumot, Laurent, additional, Yug, Lawrence, additional, Saweyog, Steven C., additional, Tided, Mary, additional, Machieng, Paul, additional, Pretrick, Moses, additional, Marfel, Maria, additional, Griggs, Anne, additional, Bel, Martin, additional, Duffy, Mark R., additional, Hancock, W. Thane, additional, Ho-Chen, Tai, additional, and Powers, Ann M., additional

Published
2014
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29. Association and birth prevalence of microcephaly attributable to Zika virus infection among infants in Paraíba, Brazil, in 2015–16: a case-control study

Author

Krow-Lucal, Elisabeth R, de Andrade, Marcia Regina, Cananéa, Juliana Nunes Abath, Moore, Cynthia A, Leite, Priscila Leal, Biggerstaff, Brad J, Cabral, Cibelle Mendes, Itoh, Megumi, Percio, Jadher, Wada, Marcelo Y, Powers, Ann M, Barbosa, Aristides, Abath, Roberta Batista, Staples, J Erin, Coelho, Giovanini Evelim, Araújo, Emerson, Medeiros, Eva Lídia Arcoverde, Brant, Jonas, Cerroni, Matheus, de Barros Moreira Beltrão, Henrique, Fantinato, Francieli Fontana Sutile Tardetti, Lise, Michael Laurence Zini, Ohara, Patrícia Miyuki, Resende, Elionardo, Saad, Eduardo, de St. Maurice, Annabelle, Dieke, Ada, Harrist, Alexia, Kwit, Natalie, Marlow, Mariel, Soke, Gnakub, de Arruda Pessoa, Roseanne, da Silva, Renata Candido, Diniz, Rogéria Chelly, de Araújo Ariette, Micheline César, Lira, Clarice França, Matos, Sandra, Wanderley, Taciana Mendonça Maia, Silva, Vanessa Oliveira Costa, da Silva, Hélio Soares, Carmo, Eduardo Hage, Carvalho, Myrian, Lentini, Nena, Miranda, Raquel, Boland, Erin, Burns, Paul, Fischer, Marc, Ledermann, Jeremy, Coronado, Fatima, Dicent-Taillepierre, Julio, Flannery, Brendan, Macedo de Oliveira, Alexandre, and Arena, José Fernando

Abstract

In 2015, the number of infants born with microcephaly increased in Paraíba, Brazil, after a suspected Zika virus outbreak. We did a retrospective case-control investigation to assess the association of microcephaly and Zika virus.

Published
2018
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32. Probing the attenuation and protective efficacy of a candidate chikungunya virus vaccine in mice with compromised interferon (IFN) signaling

Author

Partidos, Charalambos D., primary, Weger, James, additional, Brewoo, Joseph, additional, Seymour, Robert, additional, Borland, Erin M., additional, Ledermann, Jeremy P., additional, Powers, Ann M., additional, Weaver, Scott C., additional, Stinchcomb, Dan T., additional, and Osorio, Jorge E., additional

Published
2011
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34. Virulence variation among isolates of western equine encephalitis virus in an outbred mouse model

Author

Logue, Christopher H., primary, Bosio, Christopher F., additional, Welte, Thomas, additional, Keene, Kimberley M., additional, Ledermann, Jeremy P., additional, Phillips, Aaron, additional, Sheahan, Brian J., additional, Pierro, Dennis J., additional, Marlenee, Nicole, additional, Brault, Aaron C., additional, Bosio, Catharine M., additional, Singh, Amber J., additional, Powers, Ann M., additional, and Olson, Ken E., additional

Published
2009
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39. Culex Tarsalis Mosquitoes as Vectors of Highlands J Virus.

Author

Borland EM, Ledermann JP, and Powers AM

Subjects
Alphavirus genetics, Animals, Genome, Viral, Phylogeny, Saliva virology, Alphavirus isolation & purification, Culex virology, Mosquito Vectors virology
Abstract

Highlands J virus (HJV) is an alphavirus closely related to western equine encephalitis virus (WEEV) and eastern equine encephalitis virus (EEEV). HJV is an avian pathogen with the potential for disruption of poultry operations, but is not known to cause human or equine disease. HJV has only been identified in the eastern United States and is thought to have a transmission cycle similar to that of EEEV involving Culiseta melanura mosquitoes and birds. However, HJV is more genetically similar to WEEV and it remains unclear if it may be transmitted by Culex species mosquitoes like WEEV. Seven strains of HJV were characterized to assess this potential. Phylogenetic analysis of whole genome sequences revealed four distinct HJV lineages (lineages 1-4), and vector competence studies in Cx. tarsalis with four of the HJV strains from different lineages yielded two distinct infection patterns. Lineage 1 strains had low infection rates, while lineages 2 and 4 had significantly higher infection rates similar to those previously published for WEEV. The average mosquito body viral titer was highest at 8 dpi (6.60-7.26 log10 pfu equivalents/body), and head titers at all time points ranged between 6.01 and 6.80 log10 pfu equivalents/head. Nearly 45% of mosquitoes infected with strain AB-80-9 were able to transmit virus in saliva with an average titer of 5.02 log10 pfu equivalents/saliva. A single amino acid difference between high and low infectivity phenotypes was identified at genome position 8605, in the E2 gene. A nonpolar glycine was present in the low infectivity lineage 1 strains, while an acidic glutamic acid was present in the higher infectivity lineage 2 and 4 strains. This study demonstrates HJV transmission by Cx. tarsalis mosquitoes and clearly identifies the potential for transmission in the western United States. Two infection phenotypes were exhibited, indicating the need for further studies to understand Culex species transmission patterns.

Published
2016
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40. Analysis of CHIKV in Mosquitoes Infected via Artificial Blood Meal.

Author

Ledermann JP and Powers AM

Subjects
Animals, Blood Substitutes, Cells, Cultured, Chlorocebus aethiops, Insect Vectors virology, Saliva virology, Vero Cells, Aedes virology, Animal Feed virology, Chikungunya virus pathogenicity
Abstract

Having a mechanism to assess the transmission dynamics of a vector-borne virus is one critical component of understanding the life cycle of these viruses. Laboratory infection systems using artificial blood meals is one valuable approach for monitoring the progress of virus in its mosquito host and evaluating potential points for interruption of the cycle for control purposes. Here, we describe an artificial blood meal system with Chikungunya virus (CHIKV) and the processing of mosquito tissues and saliva to understand the movement and time course of virus infection in the invertebrate host.

Published
2016
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