Search

Your search keyword '"Leclercq, Céline"' showing total 124 results
Start Over Author "Leclercq, Céline"
124 results on '"Leclercq, Céline"'

10. Personality assessment with Temperament and Character Inventory in Parkinson's disease

Author

Moreau, Caroline, Defebvre, Pr Luc, Carriere, Dr Nicolas, Grolez, Dr Guillaume, Baille, Dr Guillaume, Kreisler, Dr, Jean-Pierre Pruvo, Pr, Leclerc, Pr, Lopes, Dr Renaud, Viard, Dr Romain, Kuchcinski, Dr Gregory, Dumont, Mr Julien, Dujardin, Pr Kathy, Delliaux, Mme M., Brion, Mrs M., Touzet, Dr Gustavo, Reyns, Pr Nicolas, Delval, Pr Arnaud, Santraine, Mrs Valerie, Pleuvret, Mrs Marie, Dautrevaux, Mrs Nolwen, Laugeais, Mr Victor, Ouk, Thavarak, Potey, Camille, Leclercq, Celine, Gers, Elise, Corvol, Jean-Christophe, Marie-Vidailhet, Hainque, Elodie, Welter, Marie-Laure, Lacomblez, Lucette, Grabli, David, Roze, Emmanuel, Worbe, Yulia, Delorme, Cécile, You, Hana, Ihle, Jonas, Guimeraes-Costa, Raquel, Cormier-Dequaire, Florence, Méneret, Aurélie, Hartmann, Andréas, Mariani, Louise-Laure, Lehericy, Stéphane, Czernecki, Virginie, Pineau, Fanny, Bozon, Frédérique, Huiban, Camille, Benchetrit, Eve, Karachi, Carine, Navarro, Soledad, Cornu, Philippe, Welaratne, Arlette, Dongmo-Kenfack, Carole, Mantisi, Lise, Jarry, Nathalie, Aix, Sophie, Lefort, Carine, Rouaud, Dr Tiphaine, Damier, Pr Philippe, Derkinderen, Pr Pascal, Anne-Gaelle Corbille, Dr, Calvier-Auffray, Dr Elisabeth, Rocher, Mrs Laetitia, Anne-Laure Deruet, Mrs, Sylvie, Dr Raoul, Vincent, Dr Roualdes, Le Dily Séverine, Mrs, Marques, Dr Ana, Debilly, Dr Berangere, Durif, Pr Franck, Derost, Dr Philippe, Beal, Dr Charlotte, Chassain, Carine, Delaby, Laure, Vidal, Tiphaine, Jacques Lemaire, Pr Jean, Rieu, Isabelle, Durand, Elodie, Eusebio, Pr Alexandre, Jean-Philippe Azulay, Pr, Witjas, Dr Tatiana, Fluchère, Dr Frédérique, Grimaldi, Dr Stephan, Girard, Pr Nadine, Delfini, Marie, Carron, Dr Romain, Regis, Pr Jean, Spatola, Dr Giorgio, Magnaudet, Camille, Solène, Dr Ansquer, Isabelle, Dr Benatru, Olivier, Dr Colin, Houeto Jl, Pr, Remy, Pr Guillevin, Anne, Mrs Fradet, Manssouri, Mrs Anziza, Sophie, Mrs Blondeau, Philippe, Dr Richard, Philippe, Dr Cam, Philippe, Dr Page, Benoit, Pr Bataille, Emilie, Mrs Rabois, Annie, Mrs Guillemain, Sophie, Dr Drapier, Leh, Dr Frédérique, Bonnet, Dr Alexandre, Vérin, Pr Marc, Jean-Christophe Ferré, Dr, François Houvenaghel, Mr Jean, Haegelen, Pr Claire, Kestens, Mrs Francoise, Ory, Mrs Solenn, Burbaud, Pr Pierre, Damon-Perriere, Dr Nathalie, Meissner, Pr Wassilios, Tison, Pr Francois, Bannier, Dr Stéphanie, Krim, Dr Elsa, Guehl, Pr Dominique, Molinier-Blossier, Sandrine, Ollivier, Morgan, Lacoste, Marion, Auzou, Nicolas, Bonnet, Marie, Cuny, Pr Emmanuel, Engelhardt, Dr Julien, Branchard, Olivier, Huet, Clotilde, Blanchard, Julie, Olivier, Pr Rascol, Brefel Courbon, Dr Christine, Ory Magne, Dr Fabienne, Simonetta Moreau, Dr Marion, Arbus, Pr Christophe, Bonneville, Pr Fabrice, Albert Lotterie, Dr Jean, Sarrail, Marion, Scotto d’Apollonia, Charlotte, Chaynes, Pr Patrick, Caire, Pr François, Harroch, Estelle, Maltete, Pr David, Lefaucheur, Dr Romain, Fetter, Dr Damien, Magne, Dr Nicolas, Bioux, Mrs Sandrine, Loubeyre, Mrs Maud, Bliaux, Mrs Evangéline, Pouliquen, Mrs Dorothée, Derrey, Pr Stéphane, Vernon, Mrs Linda, Ziegler, Dr Frédéric, Anheim, Mathieu, Lagha-Boukbiza, Ouhaid, Tranchant, Christine, Gebus, Odile, Montaut, Solveig, Kremer, S., Longato, Nadine, Phillips, Clélie, Voirin, Jimmy, Santin, Marie des Neiges, Chaussemy, Dominique, Mengin, Dr Amaury, Giordana, Dr Caroline, Marsé, Dr Claire, Mondot, Lydiane, Giordana, Bruno, Kardous, Robin, Bailet, Bernadette, Joly, Héloise, Fontaine, Denys, Leplus, Dr Aurélie, Faustini, Amélie, Ferrier, Vanessa, Krystkowiak, Pr Pierre, Tir, Dr Mélissa, Jean-Marc Constans, Pr, Wannepain, Sandrine, Seling, Audrey, Lefranc, Dr Michel, Blin, Stéphanie, Schuler, Béatrice, Thobois, Pr Stephane, Danaila, Dr Teodor, Laurencin, Dr Chloe, Berthezene, Pr Yves, Ameli, Dr Roxana, Klinger, Helene, Polo, Dr Gustavo, Mertens, Patrick, Nunes, A., Metereau, Elise, Hopes, Dr Lucie, Frismand, Dr Solène, Schmitt, Dr Emmanuelle, Meyer, Mrs Mylène, Dillier, Mrs Céline, Colnat, Pr Sophie, Chatelain, Mrs Anne, Philippe Brandel, Dr Jean, Hubsch, Dr Cécile, Karsenti, Dr Patte, Lebouteux, Dr Marie, Ziegler, Dr Marc, Delmaire, Dr Christine, Savatowky, Dr Julien, Vrillac, Mrs Juliette, Nakache, Mrs Claire, D'Hardemare, Dr Vincent, Belamri, Mr Lhaouas, Mesnage, Dr Valérie, Bonnet, Dr Cécilia, Correa Lino, Dr Jarbas, Jr., Decrocq, Dr Camille, Boulin, Dr Anne, Barre, Mrs Inès, Manouvrier, Mrs Jordane, Gardel, Dr Bérénice, Jarraya, Pr Béchir, Ziz, Mrs Catherine, Prette, Mrs Lydie, Douzane, Mr Hassen, Gay, David, Bonicel, Robin, El Mountassir, Fouzia, Fischer, Clara, Mangin, Jean-François, Chupin, Marie, Cointepas, Yann, Accart, Bertrand, Gelé, Patrick, Fievet, Florine, Chabel, Matthieu, Derenaucourt, Virginie, Facon, Loïc, Njosse, Yanick Tchantchou, Deplanque, Dominique, Duhamel, Alain, Djemmane, Lynda, Duflot, Florence, Boussac, Mathilde, Arbus, Christophe, Colin, Olivier, Laurencin, Chloé, Eusebio, Alexandre, Corvol, Jean Christophe, Versace, Nathalie, Rascol, Olivier, Rousseau, Vanessa, Ory-Magne, Fabienne, Fabbri, Margherita, Rolland, Anne-Sophie, Jarraya, Béchir, Maltête, David, Drapier, Sophie, Marques, Ana-Raquel, Wirth, Thomas, Meyer, Mylène, Tir, Mélissa, Rouaud, Tiphaine, Devos, David, and Brefel-Courbon, Christine

Published
2022
Full Text
View/download PDF

16. Validity and Reliability of the French Short Version of the Questionnaire of Olfactory Disorders-Negative Statements (sQOD-NS).

Author

Leclercq, Céline, Chiesa-Estomba, Carlos M., Horoi, Mihaela, Le Bon, Serge D., Hans, Stephane, Distinguin, Lea, Chekkoury-Idrissi, Younes, Circiu, Marta P., Khalife, Mohamad, Saussez, Sven, and Lechien, Jérôme R.

Subjects
*EXPERIMENTAL design, *STATISTICS, *STATISTICAL reliability, *RESEARCH methodology, *RESEARCH methodology evaluation, *VISUAL analog scale, *MANN Whitney U Test, *SEVERITY of illness index, *CRONBACH'S alpha, *QUESTIONNAIRES, *QUALITY of life, *SMELL disorders, *DESCRIPTIVE statistics, *DATA analysis software, *DATA analysis, *STATISTICAL correlation, *FRENCH people, RESEARCH evaluation
Abstract

Objective: To develop a French Short Version of the Questionnaire of Olfactory Disorders-Negative Statements (Fr-sQOD) to assess the quality of life impairments of patients with olfactory dysfunction (OD). Methods: Patients with OD and controls were enrolled from 2 academic centers. Individuals completed the Fr-sQOD, an OD visual analog scale severity, and the French version of the sinonasal outcome tool-22 (SNOT-22). Cronbach α was used to measure the internal consistency of Fr-sQOD. The reliability and the external validity of Fr-sQOD were assessed through a test–retest approach and by correlating Fr-sQOD with SNOT-22 scores, respectively. The external validity was assessed by correlation analysis between Fr-sQOD and the result of an assessment of the severity of OD on a visual analog scale. Results: Eighty patients completed the evaluations. The internal consistency was adequate (Cronbach α.96), and the test–retest reliability was high in the entire cohort (rs = 0.877, P <.001). The correlation between Fr-sQOD total scores and the severity of OD was moderate but significant (rs = −0.431; P =.001) supporting an acceptable external validity. Patients with OD had a significantly higher score of Fr-sQOD than healthy individuals (P <.001), indicating a high internal validity. Conclusion: The Fr-sQOD is a reliable and valid self-administered tool in the evaluation of the impact of OD on quality of life of French-speaking patients. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

18. Trial of Deferiprone in Parkinson’s Disease

Author

Devos, David, Labreuche, Julien, Dušek, Petr, Post, Bart, Bloem, Bastiaan R, Berg, Daniela, Maetzler, Walter, Otto, Markus, Habert, Marie-Odile, Lehericy, Stéphane, Ferreira, Joaquim, Dodel, Richard, Rascol, Olivier, Tranchant, Christine, Eusebio, Alexandre, Thobois, Stéphane, Marques, Ana-Raquel, Meissner, Wassilios G, Ory-Magne, Fabienne, Walter, Uwe, de Bie, Rob M A, Gago, Miguel, Vilas, Dolores, Corvol, Jean-Christophe, Kulisevsky, Jaime, Januario, Cristina, Coelho, Miguel V S, Behnke, Stefanie, Worth, Paul, Seppi, Klaus, Ouk, Thavarak, Potey, Camille, Leclercq, Céline, Viard, Romain, Duhamel, Alain, Kuchcinski, Gregory, Lopes, Renaud, Pruvo, Jean-Pierre, Pigny, Pascal, Garçon, Guillaume, Simonin, Ophélie, Carpentier, Jessica, Rolland, Anne-Sophie, Nyholm, Dag, Scherfler, Christoph, Guyon Delannoy, Pauline, Mangin, Jean-François, Chupin, Marie, Bordet, Régis, Dexter, David T, Fradette, Caroline, Spino, Michael, Tricta, Fernando, Ayton, Scott, Bush, Ashley I, Devedjian, Jean-Christophe, Poewe, Werner, Duce, James A, Cabantchik, Ioav, Defebvre, Luc, Deplanque, Dominique, Moreau, Caroline, Group, FAIRPARK-II Study, Compta, Yaroslau, Pavese, Nicola, Růžička, Evžen, Basenau, Sandra, Beliveau, Vincent, Benchetrit, Eve, Best, Laura, Bloem, Bas, Bonicel, Robin, Boraud, T., Bordet, Regis, Bouca, Raquel, Bourdain, Frédéric, Bouzas, Jimena, Brefel-Courbon, Christine, Bubenheim, Michael, Burn, David, Bush, Ashley I, Cabantchik, Ioav, Calvas, Fabienne, Cámara, Ana, Campolongo, Antonia, Carrière, Nicolas, Chaigneau, Véronique, Matthieu, Collin, Compta, Yaroslau, Connelly, John, Cormier-Dequaire, Florence, Cranston, Amy, Dean, Rory, De Marzi, Roberto, Degos, Bertrand, Demotes, Jacques, Dellapina, Estelle, Deplanque, Dominique, Devedjian, Jean-Christophe, Devos, David, Dexter, David, Dodel, Richard, Dongmo, Carole, Duce, James, Duhamel, Alain, Dupouy, Julia, Dusek, Petr, El Mountassir, Fouzia, Eyvrard, Frédéric, Fernández, Manel, Ferreira, Joaquim, Forni, Gian Luca, Foster, Victoria, Foubert-Samier, Alexandra, Fradette, Caroline, Fréville, Laëtitia, Galitzky, Monique, Gaudebout, Cecile, Gelé, Patrick, Giladi, Nir, Grabli, David, Gleixner, Franck, Grolez, Guillaume, Guyon, Pauline, Habert, Marie-Odile, Harroch, Estelle, Hartmann, Andreas, Hirsch, Denise, Hisbergues, Michael, Hobert, Markus A, Hopfner, Franziska, Jurado, Camille, Kaiser, Andreas, Keen, Gill, Klaus, Seppi, Kouassi, Nadège, Labreuch, Julien, Lacomblez, Lucette, Lagha Boukbiza, Ouhaid, Lanthaler, Barbara, Lechatellier, Gilles, Le Forestier, Nadine, Lehmann, Fred, Lloret, Teresa, Le Naour, J., Le Toullec, Benjamin, Locatelli, Maxime, Löhle, Matthias, Lomeña, F., Longato, Nadine, Lützen, Ulf, McNichol, Ann, Maetzler, Corina, Maetzler, Walter, Mahlknecht, Philipp, Mangone, Graziella, Marín-Lahoz, Juan, Mariani, Louise-Laure, Marques, Ana, Matei, Mihaela, Matthias, Löhle, Maucourt Bacchi, Emilie, Meissner, Wassilios, Michon, Amelie, Moreau, Caroline, Nardocci, Nardo, Nosal, Florence, Nyholm, Dag, Oeckl, Patrick, Oravska, Irena, Ory, Fabienne, Otto, Markus, Ouk, Thavarak, Pagonabarraga, Javier, Pascual-Sedano, Berta, Peball, Marina, Phillips, Clélie, Pineau, Fanny, Planellas, Lluís, Pop-Ilieva, Chiesi, Rabier, Aurélie, Olivier, D., Riedel, Christian, Rodrigues, Maura, Roullet-Solignac, Isabelle, Rose, Christian, Rozova, Anna, Růžička, Evžen, Salis, Alexandra, Schäffer, Eva, Scherfler, Christoph, Schiefermeier, Natalia, Seppi, Klaus, Smagghe, Delphine, Silva, Tânia, Silva, Pedro, Socha, Julie, Souyris, Corinne, Spampinato, Umberto, Spino, Michael, Steel, Alison, Sweta, Bajaj, Thalamas, Claire, Teodor, Danaila, Teresa, Anna, Tison, François, Tolosa, Eduardo, Tricta, Fernando, Trifirò Trifirò, Gianluca, Vidailhet, Marie, Wang, Yi, Werkmann, Mario, Yilmas, Rezzak, You, Hana, Zeuner, Kirsten, Defebvre, Luc, Rascol, Olivier, Tranchant, Christine, Eusebio, Alexandre, Thobois, Stéphane, Corvol, Jean-Christophe, Durif, Franck, Meissner, Wassilos, Yaroslau, Compta, Vilas, Dolores, Kulisevsky, Jaime, Poewe, Werner, Ruzicka, Evzen, Gago, Miguel, Januario, Cristina, Vilhena Soares Coelho, Miguel, Berg, Daniela, Behnke, Stefanie, Walter, Uwe, Worth, Paul, Pavese, Nicola, Post, Bart, de Bie, Rob M A, Abbruzzese, Giovanni, Accart, Bertrand, Allain, Marie-Anne, Anheim, Mathieu, Ardigo, Diego, Aracil-Bolaños, Ignacio, Baba, Paul, Bakker, Martijn, Balzer-Geldsetzer, Monika, Bargalló, Núria, Barone, Paolo, Neurology, ANS - Neurodegeneration, and APH - Aging & Later Life

Subjects
administration & dosage [Deferiprone], Dopamine, Dopamine Agents, Medizin, Administration, Oral, Levodopa, Antiparkinson Agents, therapeutic use [Dopamine Agents], therapeutic use [Deferiprone], Deferiprone, metabolism [Iron], adverse effects [Deferiprone], Geriatrics/Aging, Parkinson Disease, General Medicine, Disorders of movement Donders Center for Medical Neuroscience [Radboudumc 3], Hematology/Oncology, chemistry [Substantia Nigra], adverse effects [Dopamine Agents], Disease Progression, administration & dosage [Dopamine Agents], physiopathology [Parkinson Disease], adverse effects [Iron Chelating Agents], Neutropenia, analysis [Iron], administration & dosage [Antiparkinson Agents], Iron, metabolism [Parkinson Disease], Neurology/Neurosurgery, therapeutic use [Levodopa], Iron Chelating Agents, pharmacology [Iron Chelating Agents], Double-Blind Method, adverse effects [Antiparkinson Agents], Genetics, diagnostic imaging [Substantia Nigra], metabolism [Substantia Nigra], Humans, ddc:610, diagnostic imaging [Brain], therapeutic use [Antiparkinson Agents], Brain Chemistry, pharmacology [Antiparkinson Agents], therapeutic use [Iron Chelating Agents], Hematology/Oncology General, chemically induced [Neutropenia], pharmacology [Deferiprone], drug therapy [Parkinson Disease], administration & dosage [Iron Chelating Agents], Geriatrics/Aging General, pharmacology [Dopamine Agents], drug effects [Substantia Nigra], Neuroscience
Abstract

Contains fulltext : 287484.pdf (Publisher’s version ) (Open Access) BACKGROUND: Iron content is increased in the substantia nigra of persons with Parkinson's disease and may contribute to the pathophysiology of the disorder. Early research suggests that the iron chelator deferiprone can reduce nigrostriatal iron content in persons with Parkinson's disease, but its effects on disease progression are unclear. METHODS: We conducted a multicenter, phase 2, randomized, double-blind trial involving participants with newly diagnosed Parkinson's disease who had never received levodopa. Participants were assigned (in a 1:1 ratio) to receive oral deferiprone at a dose of 15 mg per kilogram of body weight twice daily or matched placebo for 36 weeks. Dopaminergic therapy was withheld unless deemed necessary for symptom control. The primary outcome was the change in the total score on the Movement Disorder Society-sponsored revision of the Unified Parkinson's Disease Rating Scale (MDS-UPDRS; range, 0 to 260, with higher scores indicating more severe impairment) at 36 weeks. Secondary and exploratory clinical outcomes at up to 40 weeks included measures of motor and nonmotor disability. Brain iron content measured with the use of magnetic resonance imaging was also an exploratory outcome. RESULTS: A total of 372 participants were enrolled; 186 were assigned to receive deferiprone and 186 to receive placebo. Progression of symptoms led to the initiation of dopaminergic therapy in 22.0% of the participants in the deferiprone group and 2.7% of those in the placebo group. The mean MDS-UPDRS total score at baseline was 34.3 in the deferiprone group and 33.2 in the placebo group and increased (worsened) by 15.6 points and 6.3 points, respectively (difference, 9.3 points; 95% confidence interval, 6.3 to 12.2; P

Published
2022

20. Nanoporous Quercetin-Loaded Silicon-Stabilized Hybrid Lipid Nanoparticles Alleviate Salt Stress in Tomato Plants

Author

Guerriero, Gea, primary, Sutera, Flavia Maria, additional, Hoffmann, Jonas, additional, Leclercq, Céline C., additional, Planchon, Sébastien, additional, Berni, Roberto, additional, Hausman, Jean-Francois, additional, Renaut, Jenny, additional, Torabi-Pour, Nissim, additional, Pennington, Holly Cherise, additional, Ahmed, Mukhtar, additional, Welsh, Michael, additional, Dehsorkhi, Ashkan, additional, and Saffie-Siebert, Suzanne, additional

Published
2023
Full Text
View/download PDF

22. Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant Haberlea rhodopensis

Author

Mladenov, Petko, primary, Zasheva, Diana, additional, Planchon, Sébastien, additional, Leclercq, Céline C., additional, Falconet, Denis, additional, Moyet, Lucas, additional, Brugière, Sabine, additional, Moyankova, Daniela, additional, Tchorbadjieva, Magdalena, additional, Ferro, Myriam, additional, Rolland, Norbert, additional, Renaut, Jenny, additional, Djilianov, Dimitar, additional, and Deng, Xin, additional

Published
2022
Full Text
View/download PDF

24. P22. The effect of dietary Laminaria digitata and CAZyme supplementation on the digestive physiology of the post-weaning piglet: A multi-disciplinary approach

Author

Miguel Ribeiro, David, primary, Filipa Pires Carvalho, Daniela, additional, Falcão Martins, Cátia, additional, Leclercq, Céline, additional, Pinho, Mário, additional, Renaut, Jenny, additional, Martinho de Almeida, André, additional, Pedro Bengala Freire, João, additional, and António Mestre Prates, José, additional

Published
2022
Full Text
View/download PDF

25. Genotype and water availability on chickpea seed yield

Author

Ribeiro, Isa C, Leclercq, Céline, Planchon, Sébastien, Simoes, Nuno, Toureiro, António, Duarte, Isabel, Costa, Joaquim Miguel, Freire, João P. B., Chaves, Manuela, Renaut, Jenny, and Pinheiro, Carla

Abstract

Grain legume seeds are staple foods with a balanced and rich nutritional composition, constituting essential sources of protein, calories, minerals and vitamins. Chickpea (Cicer arietinum L.) has one of the best nutritional compositions and ranks 3rd in world legume production. Limited water availability during seed development was proven to be deleterious to seed yield and composition, yet its effect on seed nutritional quality is unknown. Chickpea contains a number of bioactive substances with potential health-beneficial effects, still, it is considered one major food allergen in Mediterranean countries and India. We aim to evaluate the effect of the growing conditions (water availability) on the bioactive potential of chickpea seed proteins, i.e. with the potential to modulate human health when ingested.

Published
2022
Full Text
View/download PDF

26. Effect of water availability on chickpea seed yield and bioactive protein content

Author

Ribeiro, Isa C., Leclercq, Céline, Planchon, Sébastien, Simoes, Nuno, Duarte, Isabel, Freire, João P. B., Chaves, Manuela, Renaut, Jenny, and Pinheiro, Carla

Abstract

Chickpea seeds of the Elixir genotype (Portuguese elite cultivar) were produced under field conditions (INIAV breeding station, Elvas), in three consecutive but very distinct years in what concerns rainfall. Two regimes were considered – rainfed and irrigated to meet 100% of the crop water requirements. Seeds were submitted to boiling and in vitro digestion in order to simulate the potential human gastro intestinal digestion. Seed residue, total protein digestibility, nutritional quality and protein profiles were evaluated. Protein profiles from boiled and digested seed residues were obtained by 2D gel electrophoresis and RuBP staining.

Published
2022
Full Text
View/download PDF

27. Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant Haberlea Rhodopensis

Author

Mladenov, Petko Vasilev, primary, Zasheva, Diana, additional, Planchon, Sébastien, additional, Leclercq, Céline, additional, Falconet, Denis, additional, Moyet, Lucas, additional, Brugière, Sabine, additional, Moyankova, Daniela, additional, Tchorbadjieva, Magdalena, additional, Ferro, Myriam, additional, Rolland, Norbert, additional, Renaut, Jenny, additional, Djilianov, Dimitar, additional, and Deng, Xin, additional

Published
2022
Full Text
View/download PDF

30. Validity and Reliability of the French Short Version of the Questionnaire of Olfactory Disorders-Negative Statements (sQOD-NS)

Author

Leclercq, Céline, primary, Chiesa-Estomba, Carlos M., additional, Horoi, Mihaela, additional, Le Bon, Serge D., additional, Hans, Stephane, additional, Distinguin, Lea, additional, Chekkoury-Idrissi, Younes, additional, Circiu, Marta P., additional, Khalife, Mohamad, additional, Saussez, Sven, additional, and Lechien, Jérôme R., additional

Published
2021
Full Text
View/download PDF

31. Plant Extracellular Vesicles and Nanovesicles: Focus on Secondary Metabolites, Proteins and Lipids with Perspectives on Their Potential and Sources

Author

Woith, Eric, Guerriero, Gea, Hausman, Jean-Francois, Renaut, Jenny, Leclercq, Céline C., Weise, Christoph, Legay, Sylvain, Weng, Alexander, and Melzig, Matthias F.

Subjects
plant nanovesicles, in vitro plant cell culture, Proteome, Cell Culture Techniques, Secondary Metabolism, metabolomics, Article, lipids, lcsh:Chemistry, exosome-like nanoparticles, Extracellular Vesicles, Magnoliopsida, proteomics, lcsh:Biology (General), lcsh:QD1-999, 500 Naturwissenschaften und Mathematik::570 Biowissenschaften, Biologie::575 Einzelne Teile von und physiologische Systeme bei Pflanzen, Craterostigma, lcsh:QH301-705.5, Cells, Cultured, Phospholipids
Abstract

While human extracellular vesicles (EVs) have attracted a big deal of interest and have been extensively characterized over the last years, plant-derived EVs and nanovesicles have earned less attention and have remained poorly investigated. Although a series of investigations already revealed promising beneficial health effects and drug delivery properties, adequate (pre)clinical studies are rare. This fact might be caused by a lack of sources with appropriate qualities. Our study introduces plant cell suspension culture as a new and well controllable source for plant EVs. Plant cells, cultured in vitro, release EVs into the growth medium which could be harvested for pharmaceutical applications. In this investigation we characterized EVs and nanovesicles from distinct sources. Our findings regarding secondary metabolites indicate that these might not be packaged into EVs in an active manner but enriched in the membrane when lipophilic enough, since apparently lipophilic compounds were associated with nanovesicles while more hydrophilic structures were not consistently found. In addition, protein identification revealed a possible explanation for the mechanism of EV cell wall passage in plants, since cell wall hydrolases like 1,3-β-glucosidases, pectinesterases, polygalacturonases, β-galactosidases and β-xylosidase/α-L-arabinofuranosidase 2-like are present in plant EVs and nanovesicles which might facilitate cell wall transition. Further on, the identified proteins indicate that plant cells secrete EVs using similar mechanisms as animal cells to release exosomes and microvesicles.

Published
2021

32. Molecular insights into plant desiccation tolerance: transcriptomics, proteomics and targeted metabolite profiling in Craterostigma plantagineum

Author

Xu, Xuan, primary, Legay, Sylvain, additional, Sergeant, Kjell, additional, Zorzan, Simone, additional, Leclercq, Céline C., additional, Charton, Sophie, additional, Giarola, Valentino, additional, Liu, Xun, additional, Challabathula, Dinakar, additional, Renaut, Jenny, additional, Hausman, Jean‐Francois, additional, Bartels, Dorothea, additional, and Guerriero, Gea, additional

Published
2021
Full Text
View/download PDF

34. Risk factors for Coronavirus Disease 2019 (COVID-19) severity and mortality among solid cancer patients and impact of the disease on anticancer treatment: A French nationwide cohort study (GCO-002 CACOVID-19)

Author

Lièvre, Astrid, primary, Turpin, Anthony, additional, Ray-Coquard, Isabelle, additional, Le Malicot, Karine, additional, Thariat, Juliette, additional, Ahle, Guido, additional, Neuzillet, Cindy, additional, Paoletti, Xavier, additional, Bouché, Olivier, additional, Aldabbagh, Kais, additional, Michel, Pierre, additional, Debieuvre, Didier, additional, Canellas, Anthony, additional, Wislez, Marie, additional, Laurent, Lucie, additional, Mabro, May, additional, Colle, Raphael, additional, Hardy-Bessard, Anne-Claire, additional, Mansi, Laura, additional, Colomba, Emeline, additional, Bourhis, Jean, additional, Gorphe, Philippe, additional, Pointreau, Yoann, additional, Idbaih, Ahmed, additional, Ursu, Renata, additional, Di Stefano, Anna Luisa, additional, Zalcman, Gérard, additional, Aparicio, Thomas, additional, Moulin, Solenne, additional, Leleu, Olivier, additional, Leparree, Sylvie, additional, Goasdoue, Henri, additional, Piprot, Christine, additional, Tourneur, Gerald, additional, Bayart, Vincent, additional, Lignier, Delphine, additional, Lachaier, Emma, additional, Khamari, Marwa, additional, Coutte, Alexandre, additional, Siembida, Nicolas, additional, Houessinon, Aline, additional, Regimbeau, Jean Marc, additional, Chauffert, Bruno, additional, Moreira, Aurélie, additional, Hautefeuille, Vincent, additional, Hee, Christine, additional, Boone, Mathieu, additional, Bihan, Céline, additional, Chive, Emilie, additional, Poulet-Potriquier, Stéphane, additional, Fahem, Rachida, additional, Luet, Dominique, additional, Roquin, Guillaume, additional, Vitellius, Carole, additional, Cornet-Trichereau, Nathanaëlle, additional, Caroli-Bosc, François-Xavier, additional, Thirot-Bidault, Anne, additional, Ropert, Stanislas, additional, Gachet - Masson, Julie, additional, Dehais, Mélanie, additional, L'helgoualc'h, Gwen-Ael, additional, Ali-Mahamadou, Ibrahim, additional, Talfi, Safia, additional, Belmont, Laure, additional, Kilendo, Dieudonné, additional, Benrezzak, Nasro, additional, Dubief, Emeline, additional, Conroy, Guillaume, additional, Delique, Laurence, additional, Basso, Maud, additional, Pons, Isabelle, additional, Salignon, Karine, additional, Villing, Anne-Laure, additional, Mougenot, Emmanuelle, additional, Porebski, Cassandra, additional, Guiatni, Asma, additional, Cloarec, Nicolas, additional, Mineur, Laurent, additional, Bouchaud, Marie, additional, David, Céleste, additional, Peytier, Annie, additional, Greletty, Thomas, additional, Audemar, Franck, additional, Vignes, Emanuelle, additional, Minne, Floriane, additional, Goldzak, Guillaume, additional, Huysman, Fabienne, additional, Hocine, Fayçal, additional, Lakkis, Zaher, additional, Meynard, Guillaume, additional, Almotlak, Hamadi, additional, Klajer, Elodie, additional, Sun, Xu-Shan, additional, Wasselin, Julie, additional, Catala, Pascale, additional, Mazuy, Claire, additional, Vandamme, Hélène, additional, Prevost, Jean-Briac, additional, Fadin, Aurélie, additional, Basson, Laurent, additional, Huguet, Jean-Baptiste, additional, Dos Santos, Emmanuelle, additional, Jany, Bérangère, additional, Saad, Alain, additional, Goutorbe, Frédéric, additional, Oziol, Eric, additional, Ramdani, Mohamed, additional, Kadiri, Ouafae, additional, Garbay, Delphine, additional, Huet, Clotilde, additional, Giroux Leprieur, Etienne, additional, Teng, Wen, additional, Monvoisin, Justine, additional, Arnaud Coffin, Patrick, additional, Roux, Sylvie, additional, Orfeuvre, Hubert, additional, Chagros, Mélanie, additional, Pillon, Didier, additional, Rassoul, Agathe, additional, Poureau, Pierre Guillaume, additional, Novello, Cécile, additional, Ducray, François, additional, Trouba, Cécile, additional, Bastit, Vianney, additional, Babin, Emmanuel, additional, Leon, Vincent, additional, Courtecuisse, Anne-Catherine, additional, Vambre, Julie, additional, Tack, Vincent, additional, Desauw, Christophe, additional, Meniai, Fatima, additional, Peres, Christina, additional, Esparcieux, Aurélie, additional, Perrier, Hervé, additional, Doux, Nathalie, additional, Kaphan, Régis, additional, Roques, Bertrand, additional, Rebischung, Christine, additional, Mille, Dominique, additional, Fernandes, Gaëlle, additional, Abdelli, Naceur, additional, Jousset, Natacha, additional, Combe, Pierre, additional, Jonveaux, Eric, additional, Dumont, Patrick, additional, Kanaan, Marc, additional, Berthelot Gras, Corinne, additional, Panis, Valérie, additional, Kaluzinski, Laure, additional, Venant-Valery, Marjolène, additional, Lam, You-Heng, additional, Vallee, Laura, additional, Riviere, Frédéric, additional, Durand, Muriel, additional, Benghadid, Dihya, additional, Villeneuve, Emilie, additional, Hentic Dhome, Olivia, additional, Bounouar, Zedjiga, additional, De Mestier, Louis, additional, Dubois, Jacqueline, additional, Eyriey, Magali, additional, Moreau, Lionel, additional, Baihas, Dib, additional, Aldabbagh, Kaïs, additional, Degriffolet, Dominique, additional, Sebbagh, Virginie, additional, Seghezzi, Jean-Christophe, additional, Lozach-Brugirard, Marion, additional, Mandrou, Julie, additional, Mavier, Loubna, additional, Hennetier, Florence, additional, Wagner, Jean-Philippe, additional, Carola, Elisabeth, additional, Chandirakumaran, Karthiga, additional, Loutski, Sandrine, additional, Cojean-Zelek, Isabelle, additional, Bouras, Amina, additional, Lacour, Sandrine, additional, Froura, Fahem, additional, Ben Nadji, Hadjer, additional, Cattelain, Sophie, additional, Darloy, Franck, additional, Jolimoy Boilleau, Geneviève, additional, Maissiat, Cyrielle, additional, Darut-Jouve, Ariane, additional, Lorgis, Véronique, additional, Charifi-Alaoui, Ikram, additional, Ghiringhelli, François, additional, Drouillard, Antoine, additional, Chaix, Marie, additional, Manfredi, Sylvain, additional, Lepage, Côme, additional, Gagnaire, Alice, additional, Latournerie, Marianne, additional, jourdan, Sofia, additional, Perrot, Nora, additional, folia, Mireille, additional, Minello, Anne, additional, Jouve, Jean-Louis, additional, Fery, Marielle, additional, Landau, Alain, additional, Evrard, Diane, additional, Valenza, Bruno, additional, Paitel, Jean-François, additional, Chablais, Laetitia, additional, Kreitmann, Thomas, additional, Lancry-Lecomte, Laurence, additional, Monard, Adrien, additional, Faugeras, Eve, additional, Boucheret, Paul, additional, Glommeau, Cécile, additional, Tchikladze, Christine, additional, Garnier Tixidre, Claire, additional, Long, Jérôme, additional, Zaidi, Manel, additional, Delabarre, Véronique, additional, Meyzenc, Juliette, additional, Ferrand, Loïc, additional, Moro-Sibilot, Denis, additional, Bouheret, Paul, additional, Leyronnas, Cécile, additional, Herve, Camille, additional, Thoor, Audrey, additional, Jacquet, Emanuelle, additional, Roth, Gaël, additional, Madapathage-Senanyake, Videsheka, additional, Chupeau, Peggy, additional, Bieber, Elsa, additional, Rosso, Maud, additional, Lepage, Isabelle, additional, Priou, Frank, additional, Laly, Margot, additional, Aprelon, Sylvie, additional, Sobolak, Natacha, additional, Homokos, Helen, additional, Watelle, Fabienne, additional, Pham-Becker, Alice, additional, Lauridant, Géraldine, additional, Dujardin, Charlotte, additional, Lenglin, Etienne, additional, Nienguet Tsota, Aimée, additional, Dominguez, Sophie, additional, Forestier, Alexandra, additional, Nouvel, Franck, additional, Lerooy, Justine, additional, Ratajczak, Céline, additional, Romano, Olivier, additional, Brzyski, Dorothéee, additional, Barriere, Aurélien, additional, Genet, Dominique, additional, Tisse, Julien, additional, Zasadny, Xavier, additional, Grelet, Adeline, additional, Hennion-Imbault, Amélie, additional, Haustraete, Eglantine, additional, Louafi, Samy, additional, Awad, Manal, additional, Zekri, Younes, additional, Cheneau, Caroline, additional, Leissen, Nolwen, additional, Egreteau, Joëlle, additional, Breant, Alexandra, additional, Sarabi, Matthieu, additional, Labonne, Stéphanie, additional, Forestier, Julien, additional, Leclercq, Céline, additional, Prunier-Bossion, Florence, additional, Ray Coquard, Isabelle, additional, Guillet, Marielle, additional, Theillaumas, Aurélie, additional, Prome, Emilie, additional, Walter, Thomas, additional, Philouze, Pierre, additional, Lawo, Melody, additional, De Talhouet, Solène, additional, Beuvelot, Johanne, additional, Molin, Yann, additional, Bellecoste Martin, Marie, additional, Saussereau, Maud, additional, Agnelli, Lauren, additional, Fakhry, Nicolas, additional, Laplace, Christophe, additional, Norguet Monnereau, Emmanuelle, additional, Boucard, Céline, additional, Djenad, Kahina, additional, Fontaine, Catherine, additional, Seitz, Jean-François, additional, Dahan, Laétitia, additional, Sigrand, Julie, additional, Duluc, Muriel, additional, Locher, Christophe, additional, Fleury, Marjory, additional, Brou Marie, Ange, additional, Berkane, Ramdane, additional, Poupblanc, Séverine, additional, Auby, Dominique, additional, Petran, Daniela, additional, Texereau, Patrick, additional, Guerineau, Elodie, additional, Andre, Morgan, additional, Mahjoubi, Linda, additional, Sarrazin, Fanny, additional, Jeanson, Sonia, additional, Gschwend, Anthony, additional, Birr, Virginie, additional, Fore, Mathieu, additional, Noirclerc, Monique, additional, Dahou, Sihem, additional, Spaeth, Dominique, additional, Lambotin, Mélanie, additional, Lelu, Thomas, additional, Linot, Benjamin, additional, Hugon, Nathalie, additional, Rousseau, Dominique, additional, Castanie, Hélène, additional, Lenne, Carole, additional, Lortholary, Alain, additional, Cessot, Anatole, additional, Merzoug, Messaouda, additional, Naudin, Cécile, additional, Vannetzel, Jean-Michel, additional, Aziz, Ghina, additional, Hadj Arab, Yacine, additional, Pernes, Stéphanie, additional, Roche-Lachaise, Isabelle, additional, Fiteni, Frédéric, additional, Yahiaoui, Hadjer, additional, Marel Lopez, Gwendoline, additional, Oddoz, Jeanne, additional, Peira, Fabienne, additional, Michel, Olivier, additional, Meunier, Jérôme, additional, Ouahrani, Brahim, additional, Roger, Antoine, additional, Branco, Sonia, additional, Nguyen, Van, additional, Gisselbrecht, Mathilde, additional, Hammad, Ghania, additional, Mordant, Pierre, additional, Stroksztejn, Magda, additional, Pocard, Marc, additional, Nlo Meyengue, Luc, additional, Sacco, Emmanuelle, additional, Simon Anne, Sophie, additional, Fabre-Guillevin, Elizabeth, additional, Slim, Marine, additional, Zaanan, Aziz, additional, Cadranel, Jacques, additional, Pluvy, Johan, additional, Ursu, Rénata, additional, Geraldo, Amyrath, additional, Lihi, Rime, additional, Vo, Maryline, additional, Brouk, Zohra, additional, Colle, Raphaël, additional, Bennamoun, Mostefa, additional, Lacan, Fabrice, additional, Louvet, Christophe, additional, Mebarki, Soraya, additional, Veyri, Marianne, additional, Paillaud, Elena, additional, Lucas, Christelle, additional, Dubreuil, Olivier, additional, Lyamani, Jamila, additional, Agguini, Hanane, additional, Soularue, Emilie, additional, Jourdaine, Clément, additional, Verillaud, Benjamin, additional, Herzine, Hakima, additional, Raymond, Eric, additional, Mathiot, Nathalie, additional, Palmieri, Lola Jade, additional, Epanya, Christian, additional, Taieb, Julien, additional, Bertrand, Eliane, additional, Goujon, Gaël, additional, Namour, Céline, additional, Gazeau, Benoit, additional, Zafirova, Biljana, additional, Mirghani, Haitham, additional, Belin, Catherine, additional, Belkhir, Kahina, additional, Gharib, Myriam, additional, Vozy, Aurore, additional, Amrane, Karim, additional, Spano, Jean-Philippe, additional, Wassermann, Johanna, additional, Feuvret, Loic, additional, Bachet, Jean-Baptiste, additional, Philonenko, Sara, additional, Guillot, Laetitia, additional, Zabbe, Marion, additional, Gibiat, Stéphanie, additional, Baylot, Camille, additional, Jouinot, Aude, additional, Leduc, Nicolas, additional, Vieillot, Sabine, additional, James, Laurie, additional, Ducerf, Camille, additional, Blanc, Jean-Frédéric, additional, Falandry Leger, Claire, additional, Wautot, Virginie, additional, Chauvenet, Marion, additional, Vincent, Aude, additional, Tougeron, David, additional, Goulvent, Sandrine, additional, Suc, Etienne, additional, Laurenty, Anne-Pascale, additional, Marquis, Eric, additional, Bonnaire, Margaux, additional, Dewolf, Maxime, additional, Brenet, Esteban, additional, Billard, Delphine, additional, Litre, Claude-Fabien, additional, Dumazet, Antoine, additional, Botsen, Damien, additional, Vazel, Marion, additional, Carlier, Claire, additional, Bonnerave, David, additional, Marchand-Crety, Charles, additional, Bouche, Olivier, additional, Fosse, Patricia, additional, Sefrioui, David, additional, Watson, Sarah, additional, Torche, Fatah, additional, Muron, Thierry, additional, Natur, Stéphane, additional, Desgrippes, Romain, additional, Bihel, Véronique, additional, Ferrand, François-Régis, additional, Leiterer, Caroline, additional, Lavole, Julie, additional, Moquet, Claire, additional, Pressoir, Nathalie, additional, Dziukala, Catherine, additional, Ligeza Poisson, Catherine, additional, Naji, Abdelhalim, additional, Williet, Nicolas, additional, Phelip, Jean-Marc, additional, Di Palma, Fabrice, additional, Kherrour Mehdi, Amina, additional, Langrand-Escure, Julien, additional, Fournel, Pierre, additional, Pigne, Grégoire, additional, Saban-Roche, Léa, additional, Magne, Nicolas, additional, Vassal, Cécile, additional, Jacquin, Jean-Philippe, additional, Ramirez, Carole, additional, Vallard, Alexis, additional, Collard, Olivier, additional, Rivoirard, Romain, additional, Graber, Ivan, additional, Trager Maury, Stéphanie, additional, Duboisset, Elodie, additional, Ayllon Ugarte, Jorge, additional, Rami, Dalilia, additional, Saler, Christine, additional, Reinbolt, Manon, additional, Le Fevre, Clara, additional, Ben Abdelghani, Meher, additional, Dourthe, Louis-Marie, additional, Perruisseau-Carrier, Joffrey, additional, Nguimpi-Tambou, Marlène, additional, Barret, Flavie, additional, Di Stefano Anna, Luisa, additional, Balthazard, Annie, additional, Vassord-Dang, Camille, additional, Le Marchand, Mathilde, additional, Vergniol, Julien, additional, Pripon, Iulia, additional, Daemaegdt, Axelle, additional, Latry, Vanessa, additional, Larrieu, Muna, additional, Landry, Gaëlle, additional, Touihri Maximin, Laetitia, additional, Del Piano, Francesco, additional, Barlet, Agnès, additional, Vernisse, Mylène, additional, Lafond, Sophie, additional, Genin, Charline, additional, Sibertin-Blanc, Camille, additional, Chabrillac, Emilien, additional, Gregoire, Caroline, additional, Vergez, Sébastien, additional, Panouille, Quentin, additional, Guimbaud, Rosine, additional, Richa, Floriane, additional, Lebellec, Loïc, additional, Gounin, Sophie, additional, Buiret, Guillaume, additional, Baudin, Marine, additional, Hamon, Hervé, additional, Deshorgue, Anne-Claire, additional, Barrascout, Eduardo, additional, Legrand, Stéphanie, additional, Houlze, Morgane, additional, Cambula, Linda, additional, Lopez, Anthony, additional, Fouquet, Guillaume, additional, Touabi, Kahina, additional, GermaIn, Adeline, additional, Godbert, Benoit, additional, Voivret, Florence, additional, Perrin, Julie, additional, Da Silva, Rosa, additional, and Bernichon, Emilie, additional

Published
2020
Full Text
View/download PDF

35. An apoplastic fluid extraction method for the characterization of grapevine leaves proteome and metabolome from a single sample

Author

Figueiredo, Joana, primary, Cavaco, Ana Rita, additional, Guerra‐Guimarães, Leonor, additional, Leclercq, Céline, additional, Renaut, Jenny, additional, Cunha, Jorge, additional, Eiras‐Dias, José, additional, Cordeiro, Carlos, additional, Matos, Ana Rita, additional, Sousa Silva, Marta, additional, and Figueiredo, Andreia, additional

Published
2020
Full Text
View/download PDF

36. Safety of P28GST, a Protein Derived from a Schistosome Helminth Parasite, in Patients with Crohn’s Disease: A Pilot Study (ACROHNEM)

Author

Capron, Monique, primary, Béghin, Laurent, additional, Leclercq, Céline, additional, Labreuche, Julien, additional, Dendooven, Arnaud, additional, Standaert, Annie, additional, Delbeke, Marie, additional, Porcherie, Adeline, additional, Nachury, Maria, additional, Boruchowicz, Arnaud, additional, Dupas, Jean-Louis, additional, Fumery, Mathurin, additional, Paupard, Thierry, additional, Catteau, Sylviane, additional, Deplanque, Dominique, additional, Colombel, Jean-Frederic, additional, and Desreumaux, Pierre, additional

Published
2019
Full Text
View/download PDF

37. Tobacco use and related behaviors among staff and students in a university hospital: A large cross-sectional survey.

Author

Grolleau, Emmanuel, Fonteille, Véronique, Lebourgeois, Caroline, Darrason, Marie, Michel, Philippe, Ragonnet, Delphine, Freyer, Gilles, Deculty, Amélie, Gippet, Carine, Leclercq, Céline, Neugnot, Carole, Malek, Radoudja, Perdriolat, Odile, Rigaud, Michele, Torrecillas, Séverine, Barral, Maud-Catherine, Souquet, Pierre-Jean, Fassier, Jean-Baptiste, Tanguy, Lénaïck, and Rolland, Benjamin

Subjects
TOBACCO use, PSYCHOLOGY of students, UNIVERSITY hospitals, CROSS-sectional method, ELECTRONIC cigarettes
Abstract

INTRODUCTION Smoking prevalence in the overall population in France was 27% in 2017. There are few data about smoking prevalence in hospital workers. The aim of this study was to assess prevalence of current smoking in student and staff populations at Lyon University Hospital. Secondary objectives were to identify main variables associated with current smoking and willingness to quit. METHODS We designed a single center, cross-sectional survey, using printed questionnaires. During one day, all registered staff and students were surveyed. We used optical reading to extract information from questionnaires. We performed univariate and multivariate analysis adjusted on most relevant factors. RESULTS We analyzed 9712 questionnaires. The participating rates were high: 40.6% in the student cohort and 51.5% in the staff cohort. The proportion of current cigarette users was 26% in students and 25% in staff. In multivariate analysis, current smoking was significantly associated with: younger age, male sex, occupation type (e.g. logistical staff, and paramedical students), overnight work, and e-cigarette use. Among smokers, 53% reported a willingness to quit. In multivariate analysis, number of quit attempts, and feeling symptoms from tobacco were associated with willingness to quit. CONCLUSIONS Current smoking is less frequent in our cohorts of hospital staff and students than in the general French population. However, there are deep disparities in current smoking prevalence underlining a heterogeneous population. Among smokers, the majority reported a willingness to quit and some predictive factors may help to target this audience. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

38. Optimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots

Author

Abdallah Cosette, Sergeant Kjell, Guillier Christelle, Dumas-Gaudot Eliane, Leclercq Céline C, and Renaut Jenny

Subjects
Sample preparation, Membrane proteomics, Gel-free proteomics, OFFGEL peptide fractionation, iTRAQ labelling, Medicago truncatula, Cytology, QH573-671
Abstract

Abstract Background Shotgun proteomics represents an attractive technical framework for the study of membrane proteins that are generally difficult to resolve using two-dimensional gel electrophoresis. The use of iTRAQ, a set of amine-specific isobaric tags, is currently the labelling method of choice allowing multiplexing of up to eight samples and the relative quantification of multiple peptides for each protein. Recently the hyphenation of different separation techniques with mass spectrometry was used in the analysis of iTRAQ labelled samples. OFFGEL electrophoresis has proved its effectiveness in isoelectric point-based peptide and protein separation in solution. Here we describe the first application of iTRAQ-OFFGEL-LC-MS/MS on microsomal proteins from plant material. The investigation of the iTRAQ labelling effect on peptide electrofocusing in OFFGEL fractionator was carried out on Medicago truncatula membrane protein digests. Results In-filter protein digestion, with easy recovery of a peptide fraction compatible with iTRAQ labelling, was successfully used in this study. The focusing quality in OFFGEL electrophoresis was maintained for iTRAQ labelled peptides with a higher than expected number of identified peptides in basic OFFGEL-fractions. We furthermore observed, by comparing the isoelectric point (pI) fractionation of unlabelled versus labelled samples, a non-negligible pI shifts mainly to higher values. Conclusions The present work describes a feasible and novel protocol for in-solution protein digestion in which the filter unit permits protein retention and buffer removal. The data demonstrates an impact of iTRAQ labelling on peptide electrofocusing behaviour in OFFGEL fractionation compared to their native counterpart by the induction of a substantial, generally basic pI shift. Explanations for the occasionally observed acidic shifts are likewise presented.

Published
2012
Full Text
View/download PDF

39. Additional file 1: of Insights into the molecular regulation of monolignol-derived product biosynthesis in the growing hemp hypocotyl

Author

Behr, Marc, Sergeant, Kjell, Leclercq, Céline, Planchon, Sébastien, Guignard, Cédric, Lenouvel, Audrey, Renaut, Jenny, Jean-Francois Hausman, Lutts, Stanley, and Guerriero, Gea

Subjects
body regions, nervous system, fungi
Abstract

provides the protein sequences used for the phylogenetic analysis (Fig. 3). (PDF 22 kb)

Published
2018
Full Text
View/download PDF

40. An apoplastic fluid extraction method for the characterization of grapevine leaves proteome and metabolome from a single sample.

Author

Figueiredo, Joana, Cavaco, Ana Rita, Guerra‐Guimarães, Leonor, Leclercq, Céline, Renaut, Jenny, Cunha, Jorge, Eiras‐Dias, José, Cordeiro, Carlos, Matos, Ana Rita, Sousa Silva, Marta, and Figueiredo, Andreia

Subjects
VITIS vinifera, GRAPES, MASS analysis (Spectrometry), BIOLOGICAL systems, WOODY plants, MOLECULAR spectra
Abstract

The analysis of complex biological systems keeps challenging researchers. The main goal of systems biology is to decipher interactions within cells, by integrating datasets from large scale analytical approaches including transcriptomics, proteomics and metabolomics and more specialized 'OMICS' such as epigenomics and lipidomics. Studying different cellular compartments allows a broader understanding of cell dynamics. Plant apoplast, the cellular compartment external to the plasma membrane including the cell wall, is particularly demanding to analyze. Despite our knowledge on apoplast involvement on several processes from cell growth to stress responses, its dynamics is still poorly known due to the lack of efficient extraction processes adequate to each plant system. Analyzing woody plants such as grapevine raises even more challenges. Grapevine is among the most important fruit crops worldwide and a wider characterization of its apoplast is essential for a deeper understanding of its physiology and cellular mechanisms. Here, we describe, for the first time, a vacuum‐infiltration‐centrifugation method that allows a simultaneous extraction of grapevine apoplastic proteins and metabolites from leaves on a single sample, compatible with high‐throughput mass spectrometry analyses. The extracted apoplast from two grapevine cultivars, Vitis vinifera cv 'Trincadeira' and 'Regent', was directly used for proteomics and metabolomics analysis. The proteome was analyzed by nanoLC‐MS/MS and more than 700 common proteins were identified, with highly diverse biological functions. The metabolome profile through FT‐ICR‐MS allowed the identification of 514 unique putative compounds revealing a broad spectrum of molecular classes. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

43. Additional file 1: Figure S1. of Effects of silver nanoparticles and ions on a co-culture model for the gastrointestinal epithelium

Author

Georgantzopoulou, Anastasia, Serchi, Tommaso, Cambier, Sébastien, Leclercq, Céline, Renaut, Jenny, Shao, Jia, Kruszewski, Marcin, Lentzen, Esther, Grysan, Patrick, Santhana Eswara, Jean-Nicolas Audinot, Contal, Servane, Ziebel, Johanna, Guignard, Cédric, Hoffmann, Lucien, AlberTinka Murk, and Gutleb, Arno

Abstract

Mucus layer characterization (Alcian blue staining). Figure S2. Mucus layer characterization (Toluidine blue staining and TEM). Figure S3. Mucus layer characterization (Toluidine blue staining, top view). Figure S4. Cell monolayer integrity evaluation (TEER). Figure S5. Cell-free DCFH-DA assay. Figure S6. TEM images of cells in co-culture exposed to Ag particles. Figure S7. Hierarchical clustering. Table S1. Detailed information on protein identification. Table S2. Cellular Ag content determination. Table S3. KEGG enrichment analysis. (DOCX 1.17 mb)

Published
2016
Full Text
View/download PDF

45. Effects of silver nanoparticles and ions on a co-culture model for the gastrointestinal epithelium

Author

Georgantzopoulou, Anastasia, primary, Serchi, Tommaso, additional, Cambier, Sébastien, additional, Leclercq, Céline C., additional, Renaut, Jenny, additional, Shao, Jia, additional, Kruszewski, Marcin, additional, Lentzen, Esther, additional, Grysan, Patrick, additional, Eswara, Santhana, additional, Audinot, Jean-Nicolas, additional, Contal, Servane, additional, Ziebel, Johanna, additional, Guignard, Cédric, additional, Hoffmann, Lucien, additional, Murk, AlberTinka J., additional, and Gutleb, Arno C., additional

Published
2015
Full Text
View/download PDF

46. Target Tracking by Flying Drone with Fixed Camera

Author

Baccialone, Guilhem, Delaunay, Nicolas, Juan-Diego Gonzales, Leclercq, Céline, Leroux, Adrien, and Pallier, Santa

Subjects
visual servoing, stabilization, student project, Quadrotor, ComputerApplications_COMPUTERSINOTHERSYSTEMS, image processing, Unmanned Aerial Vehicles
Abstract

This paper presents the software conception of a quadrotor UAV, named SKYWATCHER, which is able to follow a target. This capacity can at a long turn time permit to follow another drone and combine their performance in order to military missions for example. From a low-cost architecture constructed by five students we implemented a software and added a camera to create a visual servoing. This project demonstrates the possibility to associate the technology of stabilization and the technology of visual enslavement., {"references":["Thesis of Celine Teuliere. « Approches deterministes et bayesiennes pour un suivi robuste: application a l'asservissement visuel d'un drone » soutenu en decembre 2010.","Henry de Plinval (ONERA). « Asservissement visuel pour drones helicopteres ».","Thesis of Ardabzgiir, «Design of control systems for a quadrotor flight vehicle equipped with inertial sensors» MecatroniqueDepartment, Atilim University.","M. Chen, M. Huzmezan, «A combined MBPC/2 DOF Hoo controller for a quadrotor UAV», Universite de la Colombie-Britannique Vancouver.","Amidi 0, Kanade T, Miller R. Vision-based autonomous helicopter research at Carnegie Mellon Robotics Institute 1991-1997: Proceedings of American Helicopter Society International Conference, Gifu, Japan, Paper no. T73, 1998.","Saripalli S, Montgomery J F, Sukhatme G S. Visually-guided landing of \nan unmanned aerial vehicle (J). IEEE Transactions on Robotics and \nAutomation, 2003, 19: 371-380.","Sharp C S, Shakenia O, Sastry S. A vision system for landing an \nunmanned aerial vehicle: Proceedings of IEEE International Conference \non Robotics and Automation, Seoul, Korea, 2001.","Jeffrey St. Clair, \"Flying Blind. The Problem with the Predator,\" \nCounterpunch (October 30, 2001).","OcéaneZubeldia, « L'armée française et l'utilisation des drones dans les \nmissions de reconnaissance, de 1960 au conflit du Kosovo », Revue \nhistorique des armées, 261 | 2010, (En ligne), mis en ligne le 10 \nnovembre 2010."]}

Published
2013
Full Text
View/download PDF

48. Effects of silver nanoparticles and ions on a co-culture model for the gastrointestinal epithelium.

Author

Georgantzopoulou, Anastasia, Serchi, Tommaso, Cambier, Sébastien, Leclercq, Céline C., Renaut, Jenny, Shao, Jia, Kruszewski, Marcin, Lentzen, Esther, Grysan, Patrick, Eswara, Santhana, Audinot, Jean-Nicolas, Contal, Servane, Ziebel, Johanna, Guignard, Cédric, Hoffmann, Lucien, Murk, AlberTinka J., and Gutleb, Arno C.

Subjects
SILVER nanoparticles, EPITHELIUM, TOLUIDINE blue, IONS, PROTEOMICS
Abstract

Background: The increased incorporation of silver nanoparticles (Ag NPs) into consumer products makes the characterization of potential risk for humans and other organisms essential. The oral route is an important uptake route for NPs, therefore the study of the gastrointestinal tract in respect to NP uptake and toxicity is very timely. The aim of the present study was to evaluate the effects of Ag NPs and ions on a Caco-2/TC7:HT29-MTX intestinal co-culture model with mucus secretion, which constitutes an important protective barrier to exogenous agents in vivo and may strongly influence particle uptake. Methods: The presence of the mucus layer was confirmed with staining techniques (alcian blue and toluidine blue). Mono and co-cultures of Caco-2/TC7 and HT29-MTX cells were exposed to Ag NPs (Ag 20 and 200 nm) and AgNO3 and viability (alamar blue), ROS induction (DCFH-DA assay) and IL-8 release (ELISA) were measured. The particle agglomeration in the media was evaluated with DLS and the ion release with ultrafiltration and ICP-MS. The effects of the Ag NPs and AgNO3 on cells in co-culture were studied at a proteome level with two-dimensional difference in gel electrophoresis (2D-DIGE) followed by Matrix Assisted Laser Desorption Ionization - Time Of Flight/ Time Of Flight (MALDI-TOF/TOF) mass spectrometry (MS). Intracellular localization was assessed with NanoSIMS and TEM. Results: The presence of mucus layer led to protection against ROS and decrease in IL-8 release. Both Ag 20 and 200 nm NPs were taken up by the cells and Ag NPs 20 nm were mainly localized in organelles with high sulfur content. A dose- and size-dependent increase in IL-8 release was observed with a lack of cytotoxicity and oxidative stress. Sixty one differentially abundant proteins were identified involved in cytoskeleton arrangement and cell cycle, oxidative stress, apoptosis, metabolism/detoxification and stress. Conclusions: The presence of mucus layer had an impact on modulating the induced toxicity of NPs. NP-specific effects were observed for uptake, pro-inflammatory response and changes at the proteome level. The low level of overlap between differentially abundant proteins observed in both Ag NPs and AgNO3 treated co-culture suggests size-dependent responses that cannot only be attributed to soluble Ag. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results