1,692 results on '"Lebon P"'
Search Results
2. Li-decorated BC3 nanopores: Promising materials for hydrogen storage
- Author
-
Cabria, I., Lebon, A., Torres, M. B., Gallego, L. J., and Vega, A.
- Subjects
Condensed Matter - Materials Science ,Condensed Matter - Mesoscale and Nanoscale Physics - Abstract
In the quest of new absorbent for hydrogen storage, we investigate the capacities of slit pores formed by two BC3 sheets decorated with Li atoms. Their hydrogen storage capacities are determined using density-functional theory in conjunction with a quantum-thermodynamic model that allows to simulate real operating conditions, i.e., finite temperatures and different loading and depletion pressures applied to the adsorbent in the charge-delivery cycles. We show that the capacities of the adsorbed hydrogen phase of Li-decorated BC3 slit pores are larger than those reported recently for graphene and Li-decorated borophene slit pores. On the other hand, the usable volumetric and gravimetric capacities of Li-decorated BC3 slit pores can meet the targets stipulated by the U.S. Department of Energy (DOE) for onboard hydrogen storage at moderate temperatures and loading pressures well below those used in the tanks employed in current technology. In particular, the usable volumetric capacity for pore widths of about 10 {\AA} meets the DOE target at a loading pressure of 6.6 MPa when depleting at ambient pressure. Our results highlight the important role played by the rotational degree of freedom of the H2 molecule in determining the confining potential within the slip pores and their hydrogen storage capacities., Comment: Article itself: 26 pages, 12 figures, 13 equations, 1 table, 78 cited articles; Supporting information: 8 pages, 6 figures, 6 equations
- Published
- 2023
3. A theoretical perspective on action consequences in action imagery: internal prediction as an essential mechanism to detect errors: a commentary on Rieger et al. 2023
- Author
-
Lebon, Florent
- Published
- 2024
- Full Text
- View/download PDF
4. Modeling of the Sump Profile During DC and MC-DC Casting Based on a Functional Packing Fraction
- Author
-
Yu, Kangcai, Wu, Yongfu, Lebon, G. S. Bruno, Patel, Jayesh B., Fan, Zhongyun, and Li, Hu-Tian
- Published
- 2024
- Full Text
- View/download PDF
5. Role of inflammation in a rat model of radiation retinopathy
- Author
-
Lebon, Cécile, Malaise, Denis, Rimbert, Nicolas, Billet, Manon, Ramasamy, Gabriel, Villaret, Jérémie, Pouzoulet, Frédéric, Matet, Alexandre, and Behar-Cohen, Francine
- Published
- 2024
- Full Text
- View/download PDF
6. Brain 18 F-FDG PET reveals cortico-subcortical hypermetabolic dysfunction in juvenile neuropsychiatric systemic lupus erythematosus
- Author
-
Rodrigo, Sebastian, Costi, Stefania, Ellul, Pierre, Aubart, Melodie, Boddaert, Nathalie, Auvin, Stephane, Elmaleh, Monique, Ntorkou, Alexandra, Bader-Meunier, Brigitte, Lebon, Vincent, Melki, Isabelle, and Chiron, Catherine
- Published
- 2024
- Full Text
- View/download PDF
7. Phototoxicity of low doses of light and influence of the spectral composition on human RPE cells
- Author
-
Françon, Anaïs, Delaunay, Kimberley, Jaworski, Thara, Lebon, Cécile, Picard, Emilie, Youale, Jenny, Behar-Cohen, Francine, and Torriglia, Alicia
- Published
- 2024
- Full Text
- View/download PDF
8. Scoping review on the role of the family doctor in the prevention and care of patients with foetal alcohol spectrum disorder
- Author
-
Leruste, Sébastien, Doray, Bérénice, Maillard, Thierry, Lebon, Christophe, Marimoutou, Catherine, and Spodenkiewicz, Michel
- Published
- 2024
- Full Text
- View/download PDF
9. Role of inflammation in a rat model of radiation retinopathy
- Author
-
Cécile Lebon, Denis Malaise, Nicolas Rimbert, Manon Billet, Gabriel Ramasamy, Jérémie Villaret, Frédéric Pouzoulet, Alexandre Matet, and Francine Behar-Cohen
- Subjects
Radiation retinopathy ,Microglia ,Inflammation ,Macrophages ,Blood retinal barrier ,Microangiopathy ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Radiation retinopathy (RR) is a major side effect of ocular tumor treatment by plaque brachytherapy or proton beam therapy. RR manifests as delayed and progressive microvasculopathy, ischemia and macular edema, ultimately leading to vision loss, neovascular glaucoma, and, in extreme cases, secondary enucleation. Intravitreal anti-VEGF agents, steroids and laser photocoagulation have limited effects on RR. The role of retinal inflammation and its contribution to the microvascular damage occurring in RR remain incompletely understood. To explore cellular and vascular events after irradiation, we analyzed their time course at 1 week, 1 month and 6 months after rat eyes received 45 Gy X-beam photons. Müller glial cells, astrocytes and microglia were rapidly activated, and these markers of retinal inflammation persisted for 6 months after irradiation. This was accompanied by early cell death in the outer retina, which persisted at later time points, leading to retinal thinning. A delayed loss of small retinal capillaries and retinal hypoxia were observed after 6 months, indicating inner blood‒retinal barrier (BRB) alteration but without cell death in the inner retina. Moreover, activated microglial cells invaded the entire retina and surrounded retinal vessels, suggesting the role of inflammation in vascular alteration and in retinal cell death. Radiation also triggered early and persistent invasion of the retinal pigment epithelium by microglia and macrophages, contributing to outer BRB disruption. This study highlights the role of progressive and long-lasting inflammatory mechanisms in RR development and demonstrates the relevance of this rat model to investigate human pathology.
- Published
- 2024
- Full Text
- View/download PDF
10. STIM2 is involved in the regulation of apoptosis and the cell cycle in normal and malignant monocytic cells
- Author
-
Stefan Djordjevic, Raphaël Itzykson, Frédéric Hague, Delphine Lebon, Julien Legrand, Hakim Ouled‐Haddou, Guillaume Jedraszak, Juliette Harbonnier, Louison Collet, Etienne Paubelle, Jean‐Pierre Marolleau, Loïc Garçon, and Thomas Boyer
- Subjects
apoptosis ,calcium ,genomic stress ,leukemia ,monocytic cells ,SOCE ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Calcium is a ubiquitous messenger that regulates a wide range of cellular functions, but its involvement in the pathophysiology of acute myeloid leukemia (AML) is not widely investigated. Here, we identified, from an analysis of The Cancer Genome Atlas and genotype‐tissue expression databases, stromal interaction molecule 2 (STIM2) as being highly expressed in AML with monocytic differentiation and negatively correlated with overall survival. This was confirmed on a validation cohort of 407 AML patients. We then investigated the role of STIM2 in cell proliferation, differentiation, and survival in two leukemic cell lines with monocytic potential and in normal hematopoietic stem cells. STIM2 expression increased at the RNA and protein levels upon monocyte differentiation. Phenotypically, STIM2 knockdown drastically inhibited cell proliferation and induced genomic stress with DNA double‐strand breaks, as shown by increased levels of phosphorylate histone H2AXγ (p‐H2AXγ), followed by activation of the cellular tumor antigen p53 pathway, decreased expression of cell cycle regulators such as cyclin‐dependent kinase 1 (CDK1)–cyclin B1 and M‐phase inducer phosphatase 3 (CDC25c), and a decreased apoptosis threshold with a low antiapoptotic/proapoptotic protein ratio. Our study reports STIM2 as a new actor regulating genomic stability and p53 response in terms of cell cycle and apoptosis of human normal and malignant monocytic cells.
- Published
- 2024
- Full Text
- View/download PDF
11. Brain 18 F-FDG PET reveals cortico-subcortical hypermetabolic dysfunction in juvenile neuropsychiatric systemic lupus erythematosus
- Author
-
Sebastian Rodrigo, Stefania Costi, Pierre Ellul, Melodie Aubart, Nathalie Boddaert, Stephane Auvin, Monique Elmaleh, Alexandra Ntorkou, Brigitte Bader-Meunier, Vincent Lebon, Isabelle Melki, and Catherine Chiron
- Subjects
Fluorodeoxyglucose ,Positron emission tomography ,Juvenile systemic lupus erythematosus ,Neuropsychiatric systemic lupus erythematosus ,Children ,Statistical parametric mapping ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
Abstract Background In juvenile systemic lupus erythematosus (j-SLE) with neuropsychiatric (NP) symptoms, there is a lack of diagnostic biomarkers. Thus, we study whether PET-FDG may identify any metabolic dysfunction in j-NPSLE. Methods A total of 19 18FDG-PET exams were consecutively performed using PET-MRI system in 11 non-sedated patients presenting with j-NPSLE (11-18y) for less than 18 months (m) and without any significant lesion at MRI. Psychiatric symptoms were scored from 0 (none) to 3 (severe) at PET time. PET images were visually analyzed and voxel-based analyses of cerebral glucose metabolism were performed using statistical parametric mapping (spm) with an age-matched control group, at threshold set > 50 voxels using both p
- Published
- 2024
- Full Text
- View/download PDF
12. Enhancing car damage repair cost prediction: Integrating ontology reasoning with regression models
- Author
-
Hamid Ahaggach, Lylia Abrouk, and Eric Lebon
- Subjects
Cost prediction ,Regression models ,SWRL ,Ontology reasoning ,Named entity recognition ,Relation extraction ,Cybernetics ,Q300-390 ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
The estimation of repair costs for car damage is a critical yet challenging task for insurance companies and repair shops. Accurate and the rapid predictions are essential for providing reliable cost estimates to customers. Traditional methods in this domain face multiple challenges, including manual processes and inaccuracies in repair cost estimation, as outlined in our article.This paper introduces a novel approach that combines regression models with ontology reasoning to enhance the accuracy of car damage repair cost predictions. An Ontology for Car Damage (OCD)11 industryportal.enit.fr/ontologies/OCD. ,22 github.com/OntologyCarDamage/OCD. has been developed, which is meticulously structured and populated using Named Entity Recognition (NER) and Relation Extraction (RE) techniques. This ontology provides a comprehensive framework for organizing and understanding the complex domain of car damage, capturing essential semantic relationships and variables that significantly influence repair costs. By integrating OCD with seven regression models, such as Random Forest and Decision Tree, we have proposed a hybrid methodology that leverages both structured data and semantic understanding. Our approach not only accounts for typical variables such as the type and severity of damage, and labor costs but also identifies novel features through the use of SWRL (Semantic Web Rule Language) rules, enhancing the model’s predictive capabilities.The performance of our models was evaluated using a substantial real-world dataset comprising over 300,000 records. This evaluation used metrics such as mean absolute error (MAE), root mean squared error (RMSE), and R-squared. The results indicate that our hybrid approach, which incorporates ontology reasoning, significantly outperforms traditional regression models.The Random Forest model, especially when combined with the OCD ontology, showcased superior performance, exhibiting a minimal average deviation from the actual repair costs and achieving a low MAE.This study’s findings demonstrate the potential of combining ontology reasoning with machine learning techniques for precise cost prediction in the automotive repair industry. Our methodology offers a robust tool for insurance companies and repair shops to generate more accurate, reliable, and automated cost estimates, ultimately benefiting both businesses and customers.
- Published
- 2024
- Full Text
- View/download PDF
13. Phototoxicity of low doses of light and influence of the spectral composition on human RPE cells
- Author
-
Anaïs Françon, Kimberley Delaunay, Thara Jaworski, Cécile Lebon, Emilie Picard, Jenny Youale, Francine Behar-Cohen, and Alicia Torriglia
- Subjects
Medicine ,Science - Abstract
Abstract Light is known to induce retinal damage affecting photoreceptors and retinal pigment epithelium. For polychromatic light, the blue part of the spectrum is thought to be the only responsible for photochemical damage, leading to the establishment of a phototoxicity threshold for blue light (445 nm). For humans it corresponds to a retinal dose of 22 J/cm2. Recent studies on rodents and non-human primates suggested that this value is overestimated. In this study, we aim at investigating the relevance of the current phototoxicity threshold and at providing new hints on the role of the different components of the white light spectrum on phototoxicity. We use an in vitro model of human induced pluripotent stem cells (hiPSC)-derived retinal pigment epithelial (iRPE) cells and exposed them to white, blue and red lights from LED devices at doses below 22 J/cm2. We show that exposure to white light at a dose of 3.6 J/cm2 induces an alteration of the global cellular structure, DNA damage and an activation of cellular stress pathways. The exposure to blue light triggers DNA damage and the activation of autophagy, while exposure to red light modulates the inflammatory response and inhibits autophagy.
- Published
- 2024
- Full Text
- View/download PDF
14. Scoping review on the role of the family doctor in the prevention and care of patients with foetal alcohol spectrum disorder
- Author
-
Sébastien Leruste, Bérénice Doray, Thierry Maillard, Christophe Lebon, Catherine Marimoutou, and Michel Spodenkiewicz
- Subjects
Family doctor ,Foetal alcohol spectrum disorder ,Prevention ,Care ,Scoping review ,Medicine (General) ,R5-920 - Abstract
Abstract Background Foetal alcohol spectrum disorder (FASD) is the leading preventable cause of nongenetic mental disability. Given the patient care pathway, the General Practitioner (GP) is in the front line of prevention and identification of FASD. Acknowledging the importance of the prevalence of FASD, general practitioners are in the front line both for the detection and diagnosis of FASD and for the message of prevention to women of childbearing age as well as for the follow-up. Objectives The main objective of the scoping review was to propose a reference for interventions that can be implemented by a GP with women of childbearing age, their partners and patients with FASD. The final aim of this review is to contribute to the improvement of knowledge and quality of care of patients with FASD. Methods A scoping review was performed using databases of peer-reviewed articles following PRISMA guidelines. The search strategy was based on the selection and consultation of articles on five digital resources. The advanced search of these publications was established using the keywords for different variations of FASD: "fetal alcohol syndrome," "fetal alcohol spectrum disorder," "general medicine," "primary care," "primary care"; searched in French and English. Results Twenty-three articles meeting the search criteria were selected. The interventions of GPs in the management of patients with FASD are multiple: prevention, identification, diagnosis, follow-up, education, and the role of coordinator for patients, their families, and pregnant women and their partners. FASD seems still underdiagnosed. Conclusion The interventions of GPs in the management of patients with FASD are comprehensive: prevention, identification, diagnosis, follow-up, education, and the role of coordinator for patients, their families, and pregnant women and their partners. Prevention interventions would decrease the incidence of FASD, thereby reducing the incidence of mental retardation, developmental delays, and social, educational and legal issues. A further study with a cluster randomized trial with a group of primary care practitioners trained in screening for alcohol use during pregnancy would be useful to measure the impact of training on the alcohol use of women of childbearing age and on the clinical status of their children.
- Published
- 2024
- Full Text
- View/download PDF
15. Seizures in Children with Influenza during the 2022–2023 Winter Season, a Case Series
- Author
-
Francesca Peranzoni, Carine Martins, Sébastien Lebon, Pierre Alex Crisinel, and Marie-Helena Perez
- Subjects
neurotropism ,influenza ,pediatric ,seizures ,Medicine (General) ,R5-920 - Abstract
Influenza is a viral infection presenting with general symptoms such as fever, headache, fatigue, and involvement of airways or the gastrointestinal tract. The nervous system may be involved, but less frequently. These neurological complications remain challenging to diagnose; moreover, no guidelines for management and treatment exist. Therefore, when presenting with neurological symptoms, patients undergo invasive diagnostic procedures and empirical treatments before making the correct diagnosis. During the winter of 2022–2023, four children between nine months and nine years of age were admitted to the Lausanne University Hospital, Switzerland, complaining of influenza and neurological complications. This report presents the symptoms of neurological manifestation and the treatment management of the four patients. All the legally authorized representatives gave their written informed consent before study inclusion.
- Published
- 2024
- Full Text
- View/download PDF
16. Novice Principal Burnout: Exploring Secondary Trauma, Working Conditions, and Coping Strategies in an Urban District. Publication
- Author
-
Texas Education Research Center, DeMatthews, David E., Reyes, Pedro, Carrola, Paul, Edwards, Wesley, and James, Lebon
- Abstract
Steady school leadership can support student achievement and equity-related outcomes, but the principalship is becoming more stressful with increasing demands, duties, and expectations. Burnout is one of several factors that contribute to principal turnover which often destabilizes a school community. Individual and organizational factors contribute to principal burnout, but remain relatively unexamined. The purpose of this mixed-methods study was to analyze the factors that contribute to burnout among novice principals in one large urban school district in the Southwestern U.S. Findings highlight the impact of secondary trauma, working conditions, and coping strategies on principal burnout.
- Published
- 2021
17. Novice Principal Burnout: Exploring Secondary Trauma, Working Conditions, and Coping Strategies in an Urban District
- Author
-
DeMatthews, David E., Reyes, Pedro, Carrola, Paul, Edwards, Wesley, and James, Lebon
- Abstract
Steady school leadership can support student achievement and equity-related outcomes, but the principalship is becoming more stressful with increasing demands, duties, and expectations. Burnout is one of several factors that contribute to principal turnover which often destabilizes a school community. Individual and organizational factors contribute to principal burnout, but remain relatively unexamined. The purpose of this mixed-methods study was to analyze the factors that contribute to burnout among novice principals in one large urban school district in the Southwestern U.S. Findings highlight the impact of secondary trauma, working conditions, and coping strategies on principal burnout.
- Published
- 2023
- Full Text
- View/download PDF
18. Accelerated aging procedure of epoxy structural adhesive for marine offshore applications
- Author
-
Marco Lamberti, Aurélien Maurel-Pantel, and Frédéric Lebon
- Subjects
Hygro-thermal environment ,Adhesive connections ,Ageing ,Mechanical properties ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Knowledge of the long-term performance of adhesive connections is undoubtedly of paramount importance to enable their deployment in civil, mechanical, and other engineering applications. Over time, adverse environmental conditions can strongly influence the performance of adhesive joints leading to a progressive deterioration of their initial mechanical properties. The use of adhesive connections for secondary structures in offshore applications is a technology that allows for the rapid creation of structural members that, however, cannot ignore the influence of hydrothermal effects on mechanical performance due to environmental conditions. In this context, the investigation of the hygrothermal durability of adhesive connections was undertaken through an extensive experimental programme. More specifically, 130 cylindrical steel joints bonded with a commercially epoxy resin for structural applications were tested in Mode I using an Arcan-modified device. Prior to test, the specimens were placed in climatic ovens capable of combining the effects of temperature and humidity for approximately 320 days. In addition, the glass transition temperature, Tg, was assessed by employing the differential scanning calorimeter (DSC) technique to correctly define the experimental ageing conditions. The experimental results show how ageing conditions influence the mechanical properties of the epoxy resin investigated. Finally, some predictive formulations are proposed to calculate the loss of strength of adhesive joints over time.
- Published
- 2024
- Full Text
- View/download PDF
19. Isotopic niches reveal the trophic structure of the cetacean community in the oceanic waters around the Azores
- Author
-
Myriam Lebon, Ana Colaço, Rui Prieto, Irma Cascão, Cláudia Oliveira, Marta Tobeña, Yann Planque, Jérôme Spitz, and Mónica A. Silva
- Subjects
stable isotopes ,marine mammals ,trophic niches ,trophic guild ,foraging ,Azores ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
IntroductionThe oceanic waters around the Azores host a high diversity of cetaceans, with 28 species of toothed and baleen whales present year-round or seasonally. This high cetacean biodiversity likely plays an important role in the structure, functioning and productivity of the ecosystem, and may increase trophic redundancy, thus contributing to food web resilience to disturbances.MethodsHere we used stable isotope (δ13C and δ15N) analysis to characterize trophic niches, assess niche overlap, describe the trophic structure and discuss potential redundancy in the cetacean community. Using 407 samples from 12 species, we estimated Standard Ellipse Areas and overlaps between species and used a hierarchical clustering analysis to identify trophic guilds.Results and discussionδ13C and δ15N values ranged from -20.53 to -15.46‰ and from 7.78 to 14.41‰ respectively, suggesting the use of diverse habitats and resources among cetacean species. Clustering analysis revealed that species were grouped into four trophic guilds, segregated mainly by trophic position (TP): a low-TP guild with three zooplanktivore baleen whales, a mid-TP guild with micronektivores, a high-TP guild with micronekton and nekton consumers, and a cluster with only Pseudorca crassidens. There was significant isotopic niche overlap between one pair of species within each guild, indicating some potential for trophic redundancy in the community. Yet, these pairs also showed some form of spatial or temporal partitioning, suggesting that mechanisms promoting species coexistence could play a key role in structuring the cetacean community in the region and in its ecological role.
- Published
- 2024
- Full Text
- View/download PDF
20. Optimizing Immuno-PET Imaging of Tumor PD-L1 Expression: Pharmacokinetic, Biodistribution, and Dosimetric Comparisons of 89Zr-Labeled Anti-PD-L1 Antibody Formats.
- Author
-
Bouleau, Alizée, Nozach, Hervé, Dubois, Steven, Kereselidze, Dimitri, Chevaleyre, Céline, Wang, Cheng-I, Evans, Michael J, Lebon, Vincent, Maillère, Bernard, and Truillet, Charles
- Subjects
Biomedical and Clinical Sciences ,Clinical Sciences ,Oncology and Carcinogenesis ,Immunology ,Biomedical Imaging ,Bioengineering ,Cancer ,Clinical Research ,Good Health and Well Being ,Animals ,B7-H1 Antigen ,Carcinoma ,Non-Small-Cell Lung ,Cell Line ,Tumor ,Humans ,Immunoglobulin G ,Lung Neoplasms ,Mice ,Mice ,Nude ,Positron Emission Tomography Computed Tomography ,Positron-Emission Tomography ,Tissue Distribution ,Zirconium ,PET ,programmed cell death ligand 1 ,PD-L1 ,immuno-therapy ,pharmacokinetics ,non-small cell lung cancer ,NSCLC ,immunotherapy ,non–small cell lung cancer ,Nuclear Medicine & Medical Imaging ,Clinical sciences - Abstract
PET imaging of programmed cell death ligand 1 (PD-L1) may help to noninvasively predict and monitor responses to anti-programmed cell death 1/anti-PD-L1 immunotherapies. In this study, we compared the imaging characteristics of 3 radioligands derived from the anti-PD-L1 IgG1 complement 4 (C4). In addition to the IgG C4, we produced a fragment antigen-binding (Fab) C4, as well as a double-mutant IgG C4 (H310A/H435Q) with minimal affinity for the murine neonatal Fc receptor. Methods: The pharmacokinetics, biodistribution, and dosimetry of the 3 89Zr-labeled C4 ligands were compared by longitudinal PET/CT imaging in nude mice bearing subcutaneous human non-small cell lung cancer xenografts with positive (H1975 model) or negative (A549 model) endogenous PD-L1 expression. Results: The C4 radioligands substantially accumulated in PD-L1-positive tumors but not in PD-L1-negative tumors or in blocked PD-L1-positive tumors, confirming their PD-L1-specific tumor targeting. 89Zr-Fab C4 and 89Zr-IgG C4 (H310A/H435Q) were rapidly eliminated compared with 89Zr-IgG C4. Consequently, maximal tumor-to-muscle ratios were obtained earlier, at 4 h after injection for 89Zr-Fab C4 (ratio, ∼6) and 24 h after injection for 89Zr-IgG C4 (H310A/H435Q) (ratio, ∼9), versus 48 h after injection for 89Zr-IgG C4 (ratio, ∼8). Background activity in nontumor tissues was low, except for high kidney retention of 89Zr-Fab C4 and persistent liver accumulation of 89Zr-IgG C4 (H310A/H435Q) compared with 89Zr-IgG C4. Dosimetry estimates suggested that the C4 radioligands would yield organ-absorbed doses tolerable for repeated clinical PET imaging studies. Conclusion: This study highlights the potential of designing radioligands with shorter pharmacokinetics for PD-L1 immuno-PET imaging in a preclinical model and encourages further clinical translation of such radioligands.
- Published
- 2022
21. French Retrospective Database Analysis of Patient Characteristics and Treatment Patterns in Patients with R/R FLT3-Mutated AML: A Registry-Based Cohort Study
- Author
-
Garnham, Andy, Bruon, Franck, Berthon, Céline, Lebon, Delphine, Parimi, Mounika, Polya, Rosalind, Makhloufi, Kahina M., and Dramard-Goasdoue, Marie-Hélène
- Published
- 2023
- Full Text
- View/download PDF
22. Early prediction of ventricular fibrillation using electrocardiographic characteristics in prehospital suspected ST-segment elevation myocardial infarction: a case–control study
- Author
-
Tanguay, Alain, Lebon, Johann, and Hébert, Denise
- Published
- 2023
- Full Text
- View/download PDF
23. First Description of a Large Clinical Series of Fetal Alcohol Spectrum Disorders Children and Adolescents in Reunion Island, France
- Author
-
Laëtitia Sennsfelder, Susie Guilly, Sonia Henkous, Christophe Lebon, Sébastien Leruste, Pauline Beuvain, Fanny Ferroul, Stéphanie Benard, Frédérique Payet, Meissa Nekaa, Maité Bagard, Magaly Lauret, Virginie Hoareau, Aurélie Caillier, Stéphanie Robin, Justine Lanneaux, Léa Etchebarren, Michel Spodenkiewicz, Jean-Luc Alessandri, Godelieve Morel, and Bérénice Roy-Doray
- Subjects
fetal alcohol spectrum disorders ,clinical description ,Reunion Island ,Pediatrics ,RJ1-570 - Abstract
Background: Despite several diagnostic guidelines, Fetal Alcohol Spectrum Disorders (FASDs) remain underdiagnosed or misdiagnosed, delaying the care of these patients and support for families. Objective: This study aims to help professionals caring for these children and their families to suspect this diagnosis earlier and to provide the most appropriate follow-up. Methods: A retrospective chart review with monocentric recruitment was performed at the Genetics Unit of the University Hospital of Reunion Island. A total of 147 children and adolescents with FASDs were included. Results: Prenatal alcohol exposure was associated with paternal alcohol consumption in 42.9%, and a high rate of prematurity (33.3%) was observed. Sixty percent of children or adolescents were placed in foster families. Learning difficulties without cognitive deficits were found in 65.8% of cases (50/76). Postural control and fine motor skills disabilities were described, respectively, in 54.7% (35/64) and 72.5% (50/69) of cases. A systematic genetic assessment was carried out, identifying in these FASD patients an associated Copy Number Variation (CNVs) in 22.6% of cases. Conclusion: Children with FASDs combine significant vulnerabilities, associating exposure to alcohol during the preconception and/or the prenatal period, prematurity, complex familial and sociocultural living conditions, and a genetic anomaly in almost a quarter of cases.
- Published
- 2024
- Full Text
- View/download PDF
24. Relevance of 18F-DOPA visual and semi-quantitative PET metrics for the diagnostic of Parkinson disease in clinical practice: a machine learning-based inference study
- Author
-
Iep, Alex, Chawki, Mohammad B., Goldfarb, Lucas, Nguyen, Loc, Brulon, Vincent, Comtat, Claude, Lebon, Vincent, and Besson, Florent L.
- Published
- 2023
- Full Text
- View/download PDF
25. What are the characteristics of disposable electronic cigarettes users in Switzerland? A quantitative study among 14–25 year olds
- Author
-
Luc Lebon, Karin Zürcher, Lorraine Chok, Diana Fernandes-Palhares, Jérémy Cros, Alexandre Dubuis, Cathy Berthouzoz, and Yara Barrense-Dias
- Subjects
Public aspects of medicine ,RA1-1270 - Abstract
Introduction Disposable electronic cigarettes (DEC) appeared in Switzerland in 2020. The aim of this study is to determine the prevalence of DEC use among youth (14–25 year olds) living in French-speaking Switzerland and their characteristics.Methods Data were obtained from a web‐based survey conducted in August 2022. Participants were divided into four DEC user groups according to and were compared on sociodemographic data, substance use, family substance use and advertising viewing. Additional questions such as purchase methods and motivations were asked to the user groups.Results Among the 1362 participants, 41.2% had never used a DEC (NEVER), 14.4% once (ONCE), 15.1% several times but not in the last 30 days (PAST) and 29.2% several times in the last 30 days (CURRENT). At the multivariate level, compared with the NEVER group, participants in the CURRENT group were more likely to have used cigarettes and cannabis in the last 30 days, to report family electronic cigarettes use and to have seen online advertising for DEC. Among DEC users, compared with the ONCE group, those in the CURRENT group were less likely to be cisgender males, to have acquired their last DEC through peers or family and to use DEC out of curiosity.Conclusions Although most youth do not consume DEC regularly, these results raise concerns about growing exposure of young people to highly addictive products. They underline the importance of monitoring products availability, marketing and prevalence. They also confirm the necessity to inform the public, denormalise use and strengthen regulations.
- Published
- 2024
- Full Text
- View/download PDF
26. Genetic diversity of endosymbiotic bacteria Wolbachia infecting two mosquito species of the genus Eretmapodites occurring in sympatry in the Comoros archipelago
- Author
-
Yann Gomard, Sarah Hafsia, Cyrille Lebon, Patrick Rabarison, Ambdoul-bar Idaroussi, Amina Yssouf, Philippe Boussès, Patrick Mavingui, and Célestine Atyame
- Subjects
Wolbachia ,Eretmapodites quinquevittatus ,Eretmapodites subsimplicipes ,mitochondrial genetic diversity ,Comoros archipelago ,Microbiology ,QR1-502 - Abstract
IntroductionThe influence of Wolbachia on mosquito reproduction and vector competence has led to renewed interest in studying the genetic diversity of these bacteria and the phenotypes they induced in mosquito vectors. In this study, we focused on two species of Eretmapodites, namely Eretmapodites quinquevittatus and Eretmapodites subsimplicipes, from three islands in the Comoros archipelago (in the Southwestern Indian Ocean).MethodsUsing the COI gene, we examined the mitochondrial genetic diversity of 879 Eretmapodites individuals from 54 sites. Additionally, we investigated the presence and genetic diversity of Wolbachia using the wsp marker and the diversity of five housekeeping genes commonly used for genotyping through Multiple Locus Sequence Typing (MLST).Results and discussionOverall, Er. quinquevittatus was the most abundant species in the three surveyed islands and both mosquito species occurred in sympatry in most of the investigated sites. We detected a higher mitochondrial genetic diversity in Er. quinquevittatus with 35 reported haplotypes (N = 615 specimens, Hd = 0.481 and π = 0.002) while 13 haplotypes were found in Er. subsimplicipes (N = 205 specimens, Hd = 0.338 and π = 0.001), this difference is likely due to the bias in sampling size between the two species. We report for the first time the presence of Wolbachia in these two Eretmapodites species. The prevalence of Wolbachia infection varied significantly between species, with a low prevalence recorded in Er. quinquevittatus (0.8%, N = 5/627) while infection was close to fixation in Er. subsimplicipes (87.7%, N = 221/252). Both male and female individuals of the two mosquito species appeared to be infected. The analysis of MLST genes revealed the presence of two Wolbachia strains corresponding to two new strain types (STs) within the supergroups A and B, which have been named wEretA and wEretB. These strains were found as mono-infections and are closely related, phylogenetically, to Wolbachia strains previously reported in Drosophila species. Finally, we demonstrate that maternal transmission of Wolbachia is imperfect in Er. subsimplicipes, which could explain the presence of a minority of uninfected individuals in the field.
- Published
- 2024
- Full Text
- View/download PDF
27. An experimental investigation of vapor compression refrigeration cooling and energy performance for CPU thermal management
- Author
-
F.M. Naduvilakath-Mohammed, M. Lebon, G. Byrne, and A.J. Robinson
- Subjects
Electronics cooling ,Vapor compression refrigeration ,Liquid cooling ,Power optimization ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
This case study experimentally investigates a R134a charged Vapor Compression Refrigeration (VCR) assisted liquid cooling of high-powered CPUs. In this hybrid system, water-based liquid cooling of the CPU is achieved in a pumped loop with a commercial cold plate affixed to the simulated CPU. The heat is then transferred to the evaporator heat exchanger of a miniature VCR system that rejects the energy to ambient air. Tests are performed at room temperature over a range of operating conditions, including CPU heat loads (12 W/cm2 - 75 W/cm2), liquid volumetric flow rates (0.5 Litre/min - 8 Litre/min), fan air speeds (2.1 m/s – 4.2 m/s), and compressor speeds (2540 rpm–6500 rpm). Both cooling performance and additional energy consumption implications are considered. Overall, it is shown that the miniature hybrid VCR system can cool the primary liquid to below ambient temperature, even at the highest tested heat load. As a result, cooling levels that far exceed both a conventional Fan-Fin Air Cooled heat exchanger and a Hybrid Liquid-Air Cooled system are achieved. Finally, optimal combinations of liquid flow rate and compressor speed are identified for minimum energy consumption of the cooling system to maintain a setpoint CPU die temperature. It is shown that management and control of pump, compressor and fan speeds can result in significant improvement in the overall system Coefficient of Performance (COP).
- Published
- 2024
- Full Text
- View/download PDF
28. Expression of placental CD146 is dysregulated by prenatal alcohol exposure and contributes in cortical vasculature development and positioning of vessel-associated oligodendrocytes
- Author
-
Camille Sautreuil, Maryline Lecointre, Jessica Dalmasso, Alexis Lebon, Matthieu Leuillier, François Janin, Matthieu Lecuyer, Soumeya Bekri, Stéphane Marret, Annie Laquerrière, Carole Brasse-Lagnel, Sophie Gil, and Bruno J. Gonzalez
- Subjects
fetal alcohol syndrome ,angiogenesis ,neurovascular development ,neuroplacentology ,biomarker ,diagnosis ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Recent data showed that prenatal alcohol exposure (PAE) impairs the “placenta–brain” axis controlling fetal brain angiogenesis in human and preclinical models. Placental growth factor (PlGF) has been identified as a proangiogenic messenger between these two organs. CD146, a partner of the VEGFR-1/2 signalosome, is involved in placental angiogenesis and exists as a soluble circulating form. The aim of the present study was to investigate whether placental CD146 may contribute to brain vascular defects described in fetal alcohol spectrum disorder. At a physiological level, quantitative reverse transcription polymerase chain reaction experiments performed in human placenta showed that CD146 is expressed in developing villi and that membrane and soluble forms of CD146 are differentially expressed from the first trimester to term. In the mouse placenta, a similar expression pattern of CD146 was found. CD146 immunoreactivity was detected in the labyrinth zone and colocalized with CD31-positive endothelial cells. Significant amounts of soluble CD146 were quantified by ELISA in fetal blood, and the levels decreased after birth. In the fetal brain, the membrane form of CD146 was the majority and colocalized with microvessels. At a pathophysiological level, PAE induced marked dysregulation of CD146 expression. The soluble form of CD146 decreased in both placenta and fetal blood, whereas it increased in the fetal brain. Similarly, the expression of several members of the CD146 signalosome, such as VEGFR2 and PSEN, was differentially impaired between the two organs by PAE. At a functional level, targeted repression of placental CD146 by in utero electroporation (IUE) of CRISPR/Cas9 lentiviral plasmids resulted in (i) a decrease in cortical vessel density, (ii) a loss of radial vascular organization, and (iii) a reduced density of oligodendrocytes. Statistical analysis showed that the more the vasculature was impaired, the more the cortical oligodendrocyte density was reduced. Altogether, these data support that placental CD146 contributes to the proangiogenic “placenta–brain” axis and that placental CD146 dysfunction contributes to the cortical oligo-vascular development. Soluble CD146 would represent a promising placental biomarker candidate representative of alcohol-induced neurovascular defects in neonates, as recently suggested by PlGF (patents WO2016207253 and WO2018100143).
- Published
- 2024
- Full Text
- View/download PDF
29. French Retrospective Database Analysis of Patient Characteristics and Treatment Patterns in Patients with R/R FLT3-Mutated AML: A Registry-Based Cohort Study
- Author
-
Andy Garnham, Franck Bruon, Céline Berthon, Delphine Lebon, Mounika Parimi, Rosalind Polya, Kahina M. Makhloufi, and Marie-Hélène Dramard-Goasdoue
- Subjects
Real-world evidence ,Treatment patterns ,Relapsed or refractory acute myeloid leukemia ,FLT3 mutations ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Introduction There is a dearth of evidence to document treatment of FMS-like tyrosine kinase 3 (FLT3)-mutated acute myeloid leukemia (AML) in real-world settings before the introduction of FLT3 inhibitors. A retrospective cohort study was conducted to understand treatment practices prior to the availability of FLT3 inhibitors in patients with FLT3-mutated AML from two registries in France. Methods Patient data from January 1, 2009 to December 31, 2017 were collected from the Hauts-de-France and Midi-Pyrénées registries. Patients aged ≥ 18 years at diagnosis with FLT3-mutated AML were included. Demographic and disease characteristics of patients with FLT3-mutated AML and relapsed or refractory (R/R) FLT3-mutated AML were documented. Treatment regimens, overall survival (OS), and event-free survival were assessed in patients with R/R FLT3-mutated AML who did not participate in clinical trials. Results Overall, 819 and 1244 adult patients with AML from the Midi-Pyrénées and Hauts-de-France cohorts, respectively, underwent FLT3 mutation testing; 172 (21.0%) and 263 (21.1%) patients, respectively, had a FLT3 mutation. Primary R/R status was identified in 41.3% (n = 71/172) of the Midi-Pyrénées and 34.6% (n = 91/263) of the Hauts-de-France cohorts. Before R/R AML diagnosis, 82.0% and 97.5% of patients in the Midi-Pyrénées and Hauts-de-France cohorts, respectively, achieved complete remission (CR) or CR with incomplete hematologic recovery (CRi) following induction chemotherapy; after diagnosis of R/R AML, CR/CRi rates with salvage therapy were 33.3% and 28.1%, respectively. Median OS (interquartile range) in patients receiving salvage therapy (n = 49, n = 78) was 5.2 (2.3–11.1) and 6.1 (2.5–35.2) months, in the Midi-Pyrénées and Hauts-de-France cohorts, respectively. Across both cohorts, patients with R/R FLT3-mutated AML had low rates of CR/CRi with salvage therapy and a median OS of approximately 6 months. Conclusion Before FLT3 inhibitor availability, real-world treatment patterns and outcomes in French patients with R/R FLT3-mutated AML were consistent with clinical trial data, highlighting a poor prognosis and unmet need for effective treatment.
- Published
- 2023
- Full Text
- View/download PDF
30. Real-world use of defibrotide for veno-occlusive disease/sinusoidal obstruction syndrome: the DEFIFrance Registry Study
- Author
-
Mohty, Mohamad, Blaise, Didier, Peffault de Latour, Régis, Labopin, Myriam, Bourhis, Jean Henri, Bruno, Benedicte, Ceballos, Patrice, Detrait, Marie, Gandemer, Virginie, Huynh, Anne, Izadifar-Legrand, Faezeh, Jubert, Charlotte, Labussière-Wallet, Hélène, Lebon, Delphine, Maury, Sébastien, Paillard, Catherine, Pochon, Cécile, Renard, Cecile, Rialland, Fanny, Schneider, Pascale, Sirvent, Anne, Asubonteng, Kobby, Guindeuil, Gwennaëlle, Yakoub-Agha, Ibrahim, and Dalle, Jean-Hugues
- Published
- 2023
- Full Text
- View/download PDF
31. The Role of Galanin in Cerebellar Granule Cell Migration in the Early Postnatal Mouse during Normal Development and After Injury
- Author
-
Komuro, Yutaro, Galas, Ludovic, Morozov, Yury M, Fahrion, Jennifer K, Raoult, Emilie, Lebon, Alexis, Tilot, Amanda K, Kikuchi, Shin, Ohno, Nobuhiko, Vaudry, David, Rakic, Pasko, and Komuro, Hitoshi
- Subjects
Biomedical and Clinical Sciences ,Neurosciences ,Stem Cell Research - Nonembryonic - Non-Human ,Stem Cell Research ,Physical Injury - Accidents and Adverse Effects ,Neurodegenerative ,2.1 Biological and endogenous factors ,Aetiology ,Neurological ,Animals ,Animals ,Newborn ,Brain ,Brain Injuries ,Cell Movement ,Cells ,Cultured ,Cerebellum ,Dose-Response Relationship ,Drug ,Female ,Galanin ,Male ,Mice ,cerebellum ,galanin ,granule cell ,live cell imaging ,mice ,neuronal migration ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Neurology & Neurosurgery - Abstract
Galanin, one of the most inducible neuropeptides, is widely present in developing brains, and its expression is altered by pathologic events (e.g., epilepsy, ischemia, and axotomy). The roles of galanin in brain development under both normal and pathologic conditions have been hypothesized, but the question of how galanin is involved in fetal and early postnatal brain development remains largely unanswered. In this study, using granule cell migration in the cerebellum of early postnatal mice (both sexes) as a model system, we examined the role of galanin in neuronal cell migration during normal development and after brain injury. Here we show that, during normal development, endogenous galanin participates in accelerating granule cell migration via altering the Ca2+ and cAMP signaling pathways. Upon brain injury induced by the application of cold insults, galanin levels decrease at the lesion sites, but increase in the surroundings of lesion sites. Granule cells exhibit the following corresponding changes in migration: (1) slowing down migration at the lesion sites; and (2) accelerating migration in the surroundings of lesion sites. Experimental manipulations of galanin signaling reduce the lesion site-specific changes in granule cell migration, indicating that galanin plays a role in such deficits in neuronal cell migration. The present study suggests that manipulating galanin signaling may be a potential therapeutic target for acutely injured brains during development.SIGNIFICANCE STATEMENT Deficits in neuronal cell migration caused by brain injury result in abnormal development of cortical layers, but the underlying mechanisms remain to be determined. Here, we report that on brain injury, endogenous levels of galanin, a neuropeptide, are altered in a lesion site-specific manner, decreasing at the lesion sites but increasing in the surroundings of lesion sites. The changes in galanin levels positively correlate with the migration rate of immature neurons. Manipulations of galanin signaling ameliorate the effects of injury on neuronal migration and cortical layer development. These results shed a light on galanin as a potential therapeutic target for acutely injured brains during development.
- Published
- 2021
32. On the Durability Performance of Two Adhesives to Be Used in Bonded Secondary Structures for Offshore Wind Installations
- Author
-
Khaoula Idrissa, Aurélien Maurel-Pantel, Frédéric Lebon, and Noamen Guermazi
- Subjects
adhesives ,thermal aging ,hygrothermal aging ,durability ,mechanical properties ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
The development of offshore wind farms requires robust bonding solutions that can withstand harsh marine conditions for the easy integration of secondary structures. This paper investigates the durability performance of two adhesives: Sikadur 30 epoxy resin and Loctite UK 1351 B25 urethane-based adhesive for use in offshore wind environments. Tensile tests on adhesive samples and accelerated aging tests were carried out under a variety of temperatures and environmental conditions, including both dry and wet conditions. The long-term effects of aging on adhesive integrity are investigated by simulating the operational life of offshore installations. The evolution of mechanical properties, studied under accelerated aging conditions, provides an important indication of the longevity of structures under normal conditions. The results show significant differences in performance between the two adhesives, highlighting their suitability for specific operating parameters. It should also be noted that for both adhesives, their exposure to different environments (seawater, distilled water, humid climate) over a prolonged period showed that (i) Loctite adhesive has a slightly faster initial uptake than Sikadur adhesive, but the latter reaches an asymptotic plateau with a lower maximum absorption rate than Loctite adhesive; and (ii) a progressive deterioration in the tensile properties occurred following an exponential function. Therefore, aging behavior results showed a clear correlation with the Arrhenius law, providing a predictive tool for the aging process and the aging process of the two adhesives followed Arrhenius kinetics. Ultimately, the knowledge gained from this study is intended to inform best practice in the use of adhesives, thereby improving the reliability and sustainability of the offshore renewable energy infrastructure.
- Published
- 2024
- Full Text
- View/download PDF
33. Steering G protein activation by mGlu heterodimer
- Author
-
Lebon, Guillaume
- Published
- 2023
- Full Text
- View/download PDF
34. Impressive extramedullary plasmacytoma response in refractory multiple myeloma treated with teclistamab
- Author
-
Intissar Ben Salah, Lydiane Mordier, Charlotte Leleux, Clément Gourguechon, Delphine Lebon, and Lydia Montes
- Subjects
Diseases of the blood and blood-forming organs ,RC633-647.5 - Published
- 2024
- Full Text
- View/download PDF
35. Non-interventional Study Evaluating the Mobilization of Stem Cells by Plerixafor Before Salvage Autologous Stem Cell Transplant in Relapsed Multiple Myeloma (IFM-2015-03)
- Author
-
van de Wyngaert, Zoe, Malard, Florent, Hulin, Cyrille, Caillot, Denis, Mariette, Clara, Facon, Thierry, Touzeau, Cyrille, Perrot, Aurore, Moreau, Philippe, Hebraud, Benjamin, Kanouni, Tarik, Heshmati, Farhad, Lebon, Delphine, Mohty, Mohamad, and Chabannon, Christian
- Published
- 2023
- Full Text
- View/download PDF
36. Targeting the brain 5-HT7 receptor to prevent hypomyelination in a rodent model of perinatal white matter injuries
- Author
-
Bokobza, Cindy, Jacquens, Alice, Guenoun, David, Bianco, Blandine, Galland, Anne, Pispisa, Maxime, Cruz, Alexandra, Zinni, Manuela, Faivre, Valérie, Roumier, Anne, Lebon, Sophie, Vitalis, Tania, Csaba, Zsolt, Le Charpentier, Tifenn, Schwendimann, Leslie, Young-Ten, Pierrette, Degos, Vincent, Monteiro, Patricia, Dournaud, Pascal, Gressens, Pierre, and Van Steenwinckel, Juliette
- Published
- 2023
- Full Text
- View/download PDF
37. Dipalmitoyl-phosphatidylserine-filled cationic maltodextrin nanoparticles exhibit enhanced efficacy for cell entry and intracellular protein delivery in phagocytic THP-1 cells
- Author
-
Brinkhuizen Clément, Shapman Damien, Lebon Alexis, Bénard Magalie, Tardivel Meryem, Dubuquoy Laurent, Galas Ludovic, and Carpentier Rodolphe
- Subjects
nanoparticle ,vaccine ,phospholipids ,efferocytosis ,targeting ,Biology (General) ,QH301-705.5 - Abstract
Vaccination through the upper respiratory tract is a promising strategy, and particulate antigens, such as antigens associated with nanoparticles, triggered a stronger immune response than the sole antigens. Cationic maltodextrin-based nanoparticles loaded with phosphatidylglycerol (NPPG) are efficient for intranasal vaccination but non-specific to trigger immune cells. Here we focused on phosphatidylserine (PS) receptors, specifically expressed by immune cells including macrophages, to improve nanoparticle targeting through an efferocytosis-like mechanism. Consequently, the lipids associated with NPPG have been substituted by PS to generate cationic maltodextrin-based nanoparticles with dipalmitoyl-phosphatidylserine (NPPS). Both NPPS and NPPG exhibited similar physical characteristics and intracellular distribution in THP-1 macrophages. NPPS cell entry was faster and higher (two times more) than NPPG. Surprisingly, competition of PS receptors with phospho-L-serine did not alter NPPS cell entry and annexin V did not preferentially interact with NPPS. Although the protein association is similar, NPPS delivered more proteins than NPPG in cells. On the contrary, the proportion of mobile nanoparticles (50%), the movement speed of nanoparticles (3 µm/5 min), and protein degradation kinetics in THP-1 were not affected by lipid substitution. Together, the results indicate that NPPS enter cells and deliver protein better than NPPG, suggesting that modification of the lipids of cationic maltodextrin-based nanoparticles may be a useful strategy to enhance nanoparticle efficacy for mucosal vaccination.
- Published
- 2023
- Full Text
- View/download PDF
38. Strontium isotope evidence for Pre-Islamic cotton cultivation in Arabia
- Author
-
Saskia E. Ryan, Eric Douville, Arnaud Dapoigny, Pierre Deschamps, Vincent Battesti, Abel Guihou, Matthieu Lebon, Jérôme Rohmer, Vladimir Dabrowski, Patricia Dal Prà, Laïla Nehmé, Antoine Zazzo, and Charlène Bouchaud
- Subjects
cotton (Gossypium arboreum/herbaceum) ,strontium isotopes ,provenance ,Nabatean Kingdom ,Hegra ,Dadan ,Science - Abstract
With a view to understanding the dynamics of ancient trade and agrobiodiversity, archaeobotanical remains provide a means of tracing the trajectories of certain agricultural commodities. A prime example is cotton in Arabia, a plant that is non-native but has been found in raw seed and processed textile form at Hegra and Dadan, in the region of al-ʿUlā, north-western Saudi Arabia—sites of critical importance given their role in the trans-Arabian trading routes during Antiquity. Here, we demonstrate that the measurement of strontium isotopes from pre-cleaned archaeological cotton is methodologically sound and is an informative addition to the study of ancient plant/textile provenance, in this case, putting forward evidence for local production of cotton in oasis agrosystems and possible external supply. The presence of locally-grown cotton at these sites from the late 1st c. BCE–mid 6th c. CE is significant as it demonstrates that cotton cultivation in Arabia was a Pre-Islamic socio-technical feat, while imported cotton highlights the dynamism of trade at that time.
- Published
- 2023
- Full Text
- View/download PDF
39. Local and distant response to intratumoral immunotherapy assessed by immunoPET in mice
- Author
-
Aurélien Marabelle, Louis Meyblum, Lambros Tselikas, Thierry de Baere, Sandrine Susini, Fréderic Deschamps, Baptiste Bonnet, Céline Chevaleyre, Benoit Jego, Dimitri Kereselidze, Vincent Lebon, and Charles Truillet
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background Despite the promising efficacy of immune checkpoint blockers (ICB), tumor resistance and immune-related adverse events hinder their success in cancer treatment. To address these challenges, intratumoral delivery of immunotherapies has emerged as a potential solution, aiming to mitigate side effects through reduced systemic exposure while increasing effectiveness by enhancing local bioavailability. However, a comprehensive understanding of the local and systemic distribution of ICBs following intratumoral administration, as well as their impact on distant tumors, remains crucial for optimizing their therapeutic potential.To comprehensively investigate the distribution patterns following the intratumoral and intravenous administration of radiolabeled anti-cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and to assess its corresponding efficacy in both injected and non-injected tumors, we conducted an immunoPET imaging study.Methods CT26 and MC38 syngeneic colorectal tumor cells were implanted subcutaneously on both flanks of Balb/c and C57Bl/6 mice, respectively. Hamster anti-mouse CTLA-4 antibody (9H10) labeled with zirconium-89 ([89Zr]9H10) was intratumorally or intravenously administered. Whole-body distribution of the antibody was monitored by immunoPET imaging (n=12 CT26 Balb/c mice, n=10 MC38 C57Bl/6 mice). Tumorous responses to injected doses (1–10 mg/kg) were correlated with specific uptake of [89Zr]9H10 (n=24). Impacts on the tumor microenvironment were assessed by immunofluorescence and flow cytometry.Results Half of the dose was cleared into the blood 1 hour after intratumoral administration. Despite this, 7 days post-injection, 6–8% of the dose remained in the intratumoral-injected tumors. CT26 tumors with prolonged ICB exposure demonstrated complete responses. Seven days post-injection, the contralateral non-injected tumor uptake of the ICB was comparable to the one achieved through intravenous administration (7.5±1.7% ID.cm–3 and 7.6±2.1% ID.cm–3, respectively) at the same dose in the CT26 model. This observation was confirmed in the MC38 model. Consistent intratumoral pharmacodynamic effects were observed in both intratumoral and intravenous treatment groups, as evidenced by a notable increase in CD8+T cells within the CT26 tumors following treatment.Conclusions ImmunoPET-derived pharmacokinetics supports intratumoral injection of ICBs to decrease systemic exposure while maintaining efficacy compared with intravenous. Intratumoral-ICBs lead to high local drug exposure while maintaining significant therapeutic exposure in non-injected tumors. This immunoPET approach is applicable for clinical practice to support evidence-based drug development.
- Published
- 2023
- Full Text
- View/download PDF
40. G protein-coupled receptor 17 is regulated by WNT pathway during oligodendrocyte precursor cell differentiation
- Author
-
Marta Boccazzi, Giulia Macchiarulo, Sophie Lebon, Justyna Janowska, Tifenn Le Charpentier, Valérie Faivre, Jennifer Hua, Davide Marangon, Davide Lecca, Marta Fumagalli, Shyamala Mani, Maria P. Abbracchio, Pierre Gressens, Anne-Laure Schang, and Juliette Van Steenwinckel
- Subjects
White matter injury ,Oligodendrocytes ,GPR17 ,GPR17 promoter ,WNT ,ID2 ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
G protein-coupled receptor 17 (GPR17) and the WNT pathway are critical players of oligodendrocyte (OL) differentiation acting as essential timers in developing brain to achieve fully-myelinating cells. However, whether and how these two systems are related to each other is still unknown. Of interest, both factors are dysregulated in developing and adult brain diseases, including white matter injury and cancer, making the understanding of their reciprocal interactions of potential importance for identifying new targets and strategies for myelin repair. Here, by a combined pharmacological and biotechnological approach, we examined regulatory mechanisms linking WNT signaling to GPR17 expression in OLs. We first analyzed the relative expression of mRNAs encoding for GPR17 and the T cell factor/Lymphoid enhancer-binding factor-1 (TCF/LEF) transcription factors of the canonical WNT/β-CATENIN pathway, in PDGFRα+ and O4+ OLs during mouse post-natal development. In O4+ cells, Gpr17 mRNA level peaked at post-natal day 14 and then decreased concomitantly to the physiological uprise of WNT tone, as shown by increased Lef1 mRNA level. The link between WNT signaling and GPR17 expression was further reinforced in vitro in primary PDGFRα+ cells and in Oli-neu cells. High WNT tone impaired OL differentiation and drastically reduced GPR17 mRNA and protein levels. In Oli-neu cells, WNT/β-CATENIN activation repressed Gpr17 promoter activity through both putative WNT response elements (WRE) and upregulation of the inhibitor of DNA-binding protein 2 (Id2). We conclude that the WNT pathway influences OL maturation by repressing GPR17, which could have implications in pathologies characterized by dysregulations of the OL lineage including multiple sclerosis and oligodendroglioma.
- Published
- 2023
- Full Text
- View/download PDF
41. Well-posedness for the coupling of a random heat equation with a multiplicative stochastic Barenblatt equation
- Author
-
Bauzet, Caroline, Lebon, Frédéric, Maitlo, Asghar Ali, and Zimmermann, Aleksandra
- Subjects
Mathematics - Analysis of PDEs - Abstract
In this contribution, a stochastic nonlinear evolution system under Neumann boundary conditions is investigated. Precisely, we are interested in finding an existence and uniqueness result for a random heat equation coupled with a Barenblatt's type equation with a multiplicative stochastic force in the sense of It\^o. In a first step we establish well-posedness in the case of an additive noise through a semi-implicit time discretization of the system. In a second step, the derivation of continuous dependence estimates of the solution with respect to the data allows us to show the desired existence and uniqueness result for the multiplicative case.
- Published
- 2019
42. Non-interventional Study Evaluating the Mobilization of Stem Cells by Plerixafor Before Salvage Autologous Stem Cell Transplant in Relapsed Multiple Myeloma (IFM-2015-03)
- Author
-
Zoe van de Wyngaert, Florent Malard, Cyrille Hulin, Denis Caillot, Clara Mariette, Thierry Facon, Cyrille Touzeau, Aurore Perrot, Philippe Moreau, Benjamin Hebraud, Tarik Kanouni, Farhad Heshmati, Delphine Lebon, Mohamad Mohty, and Christian Chabannon
- Subjects
Plerixafor ,Salvage autologous transplant ,Relapsed multiple myeloma ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Abstract Introduction Despite the implementation of new therapeutic agents, management of relapsed multiple myeloma (MM) remains a challenge. Salvage autologous hematopoietic cell transplant (AHCT) remains a valid therapeutic option for eligible patients who achieve prolonged response after a first AHCT. However, a second graft is not always available, and these patients may need a second mobilization. Patients and Methods This prospective, non-interventional, multicenter study aimed to collect data on the feasibility of salvage AHCT using a plerixafor-based hematopoietic cell mobilization in relapsed MM, according to the plerixafor label in France. Adult patients with relapsed MM eligible for a second AHCT and mobilized using granulocyte- colony stimulating factor (G-CSF) and plerixafor were included. Results Of the 23 patients, 17 achieved a successful hematopoietic cell mobilization and 13 were able to proceed to a second AHCT. Median age was 62.9 years (min–max 51–71). Ten patients (77%) were male. Eleven (85%) received AHCT as a third-line treatment or more. Median time between first and second AHCT was 5.4 years (range, 2.6–16.3). Among 18 evaluable patients, mobilization was successful for 17 (94%) of them [95% CI 84–100], with no reported side effects. Among the 13 patients who underwent salvage AHCT, the median time to engraftment was 14 days (min–max 11–29). One-year progression-free and overall survival were 88.9% [95% CI 43.3–98.4] and 100%, respectively. Conclusion This study demonstrated that plerixafor allows safe and efficient mobilization in relapsed MM patients who are candidates for a salvage AHCT. Trial Registration NCT02439476 Registered 8 May 2015, https://clinicaltrials.gov/ct2/show/NCT02439476 .
- Published
- 2023
- Full Text
- View/download PDF
43. Relevance of 18F-DOPA visual and semi-quantitative PET metrics for the diagnostic of Parkinson disease in clinical practice: a machine learning-based inference study
- Author
-
Alex Iep, Mohammad B. Chawki, Lucas Goldfarb, Loc Nguyen, Vincent Brulon, Claude Comtat, Vincent Lebon, and Florent L. Besson
- Subjects
Fluorodopa F 18 ,Parkinson's disease ,Machine learning ,Positron-emission tomography ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
Abstract Purpose To decipher the relevance of visual and semi-quantitative 6-fluoro-(18F)-L-DOPA (18F-DOPA) interpretation methods for the diagnostic of idiopathic Parkinson disease (IPD) in hybrid positron emission tomography (PET) and magnetic resonance imaging. Material and methods A total of 110 consecutive patients (48 IPD and 62 controls) with 11 months of median clinical follow-up (reference standard) were included. A composite visual assessment from five independent nuclear imaging readers, together with striatal standard uptake value (SUV) to occipital SUV ratio, striatal gradients and putamen asymmetry-based semi-quantitative PET metrics automatically extracted used to train machine learning models to classify IPD versus controls. Using a ratio of 70/30 for training and testing sets, respectively, five classification models—k-NN, LogRegression, support vector machine, random forest and gradient boosting—were trained by using 100 times repeated nested cross-validation procedures. From the best model on average, the contribution of PET parameters was deciphered using the Shapley additive explanations method (SHAP). Cross-validated receiver operating characteristic curves (cv-ROC) of the most contributive PET parameters were finally estimated and compared. Results The best machine learning model (k-NN) provided final cv-ROC of 0.81. According to SHAP analyses, visual PET metric was the most important contributor to the model overall performance, followed by the minimum between left and right striatal to occipital SUV ratio. The 10-time cv-ROC curves of visual, min SUVr or both showed quite similar performance (mean area under the ROC of 0.81, 0.81 and 0.79, respectively, for visual, min SUVr or both). Conclusion Visual expert analysis remains the most relevant parameter to predict IPD diagnosis at 11 months of median clinical follow-up in 18F-FDOPA. The min SUV ratio appears interesting in the perspective of simple semi-automated diagnostic workflows.
- Published
- 2023
- Full Text
- View/download PDF
44. Retelling the Story of the 2017 French Presidential Election: The contribution of Approval Voting
- Author
-
Baujard, Antoinette and Lebon, Isabelle
- Published
- 2022
- Full Text
- View/download PDF
45. Fitness costs associated with a GABA receptor mutation conferring dieldrin resistance in Aedes albopictus
- Author
-
Gomard, Yann, Alout, Haoues, Lebon, Cyrille, Latreille, Anne, Benlali, Aude, Mavingui, Patrick, Tortosa, Pablo, and Atyame, Célestine
- Published
- 2022
- Full Text
- View/download PDF
46. A pharmacological imaging challenge based on 11C-buprenorphine PET-MRI to explore the response to opioids in humans
- Author
-
Leroy, Claire, Goutal, Sébastien, Breuil, Louise, Gervais, Philippe, Cherkaoui, Hamza, Ciuciu, Philippe, Auvity, Sylvain, Vodovar, Dominique, Comtat, Claude, Lebon, Vincent, Bottlaender, Michel, and Tournier, Nicolas
- Published
- 2023
- Full Text
- View/download PDF
47. Comparison of two Phaeodactylum tricornutum ecotypes under nitrogen starvation and resupply reveals distinct lipid accumulation strategies but a common degradation process
- Author
-
Victor Murison, Josiane Hérault, Martine Côme, Sabrina Guinio, Alexis Lebon, Christophe Chamot, Magalie Bénard, Ludovic Galas, Benoît Schoefs, Justine Marchand, Muriel Bardor, and Lionel Ulmann
- Subjects
diatom ,Phaeodactylum ,lipid droplet ,lipid catabolism ,chloroplast ,stress ,Plant culture ,SB1-1110 - Abstract
IntroductionPhaeodactylum tricornutum is a model species frequently used to study lipid metabolism in diatoms. When exposed to a nutrient limitation or starvation, diatoms are known to accumulate neutral lipids in cytoplasmic lipid droplets (LDs). Those lipids are produced partly de novo and partly from the recycle of plastid membrane lipids. Under a nitrogen resupply, the accumulated lipids are catabolized, a phenomenon about which only a few data are available. Various strains of P. tricornutum have been isolated around the world that may differ in lipid accumulation patterns.MethodsTo get further information on this topic, two genetically distant ecotypes of P. tricornutum (Pt1 and Pt4) have been cultivated under nitrogen deprivation during 11 days followed by a resupply period of 3 days. The importance of cytoplasmic LDs relative to the plastid was assessed by a combination of confocal laser scanning microscopy and cell volume estimation using bright field microscopy pictures.Results and discussionWe observed that in addition to a basal population of small LDs (0.005 μm3 to 0.7 μm3) present in both strains all along the experiment, Pt4 cells immediately produced two large LDs (up to 12 μm3 after 11 days) while Pt1 cells progressively produced a higher number of smaller LDs (up to 7 μm3 after 11 days). In this work we showed that, in addition to intracellular available space, lipid accumulation may be limited by the pre-starvation size of the plastid as a source of membrane lipids to be recycled. After resupplying nitrogen and for both ecotypes, a fragmentation of the largest LDs was observed as well as a possible migration of LDs to the vacuoles that would suggest an autophagic degradation. Altogether, our results deepen the understanding of LDs dynamics and open research avenues for a better knowledge of lipid degradation in diatoms.
- Published
- 2023
- Full Text
- View/download PDF
48. P776: ATGAM EFFICACY AND SAFETY IN MODERATE AND SEVERE ACQUIRED APLASTIC ANEMIA: OUTCOME OF A LARGE MULTICENTER COHORT OF 634 CHILDREN AND ADULTS FROM THE FRENCH AUTHORIZATION FOR TEMPORARY USE SURVEILLANCE PROGRAM
- Author
-
Flore Sicre de Fontbrune, Mony Fahd, Edouard Forcade, Suzanne Tavitian, Cécile Moluçon-Chabrot, Fiorenza Barraco, Yosr Hicheri, Delphine Lebon, Sébastien Maury, Anne-Lise Menard, Barbara Możejko-Pastewka, Kevin Wolter, Bruno Valtier, Thierry Leblanc, and Régis Peffault de Latour
- Subjects
Diseases of the blood and blood-forming organs ,RC633-647.5 - Published
- 2023
- Full Text
- View/download PDF
49. PB1769: STIM2, A CALCIUM CENSOR POTENTIALLY INVOLVED IN THE PATHOPHYSIOLOGY OF ACUTE MYELOID LEUKEMIA
- Author
-
Stefan Djordjevic, Delphine Lebon, Raphael Itzykson, Hakim Ouled-Haddou, Nicolas Duployer, Juliette Harbonnier, Louison Collet, Etienne Paubelle, Guillaume Jedraszak, Jean Pierre Marolleau, Thomas Boyer, and Loic Garcon
- Subjects
Diseases of the blood and blood-forming organs ,RC633-647.5 - Published
- 2023
- Full Text
- View/download PDF
50. P1104: THE PROGNOSTIC ROLE OF DEPTH OF RESPONSE DEPENDS ON THE TIME OF ASSESSMENT AFTER FIRST-LINE IMMUNOCHEMOTHERAPY IN PATIENTS WITH SYMPTOMATIC WALDENSTROM MACROGOBULINEMIA (WM).
- Author
-
Lydia Montes, Caroline Delette, Daniela Robu, Delphine Lebon, Etienne Paubelle, Jean Pierre Marolleau, and Pierre Morel
- Subjects
Diseases of the blood and blood-forming organs ,RC633-647.5 - Published
- 2023
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.