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ABSTRACT Extensive foliar shedding in monoculture soybeans post‐anthesis negatively impacts yield, whereas relay strip intercropping prolongs leaf area duration, enhancing productivity. However, little is known about the causes of leaf shedding in monoculture and its impact on physiological functions and plasticity of source and sink organs, we conducted a 4‐year field experiment and leaf‐removal simulations in relay intercropped soybeans. Results revealed that monoculture soybeans experienced severe self‐shading and defoliation, while relay intercropping maintained better light conditions, supporting higher leaf area, nodule numbers, and carbon allocation. Increasing leaf removal initially increased leaf area but eventually reduced it. Extensive leaf‐removal reduced Rubisco and sucrose phosphate synthase (SPS) activity, as well as sucrose, malate, ATP, and energy charge (EC) in nodules, revealing a trade‐off between leaf growth and nodule development. Moderate leaf‐removal (L30), however, balanced compensation and consumption, increasing total non‐structural carbohydrates (TNC) in roots and N and ureide in leaves and pods. Network analysis showed that L30 improved the synergies of functional traits in leaves and nodules, ultimately benefiting overall plant growth and nutrient accumulation in pods. This study elucidates a mechanism of foliar shedding and highlights how relay strip intercropping optimizes source‐sink coordination to enhance photosynthesis and nitrogen fixation. [ABSTRACT FROM AUTHOR]

Background and Aims Leaf elongation is vital for productivity of Poaceae species, influenced by atmospheric CO2 concentration ([CO2]) and climate-induced water availability changes. Although [CO2] mitigates the effects of drought on reducing transpiration per unit leaf area, it also increases total leaf area and water use. These complex interactions associated with leaf growth pose challenges in anticipating climate change effects. This study aims to assess [CO2] effects on leaf growth response to drought in perennial ryegrass (Lolium perenne), tall fescue (Festuca arundinacea) and wheat (Triticum aestivum). Methods Plants were cultivated in growth chambers with [CO2] at 200 or 800 ppm. At leaf six to seven unfolding, half of the plants were subjected to severe drought treatment. Leaf elongation rate (LER) was measured daily, whereas plant transpiration was continuously recorded gravimetrically. Additionally, water-soluble carbohydrate (WSC) content along with water and osmotic potentials in the leaf growing zone were measured at drought onset, mid-drought and leaf growth cessation. Key Results Elevated [CO2] mitigated drought impacts on LER and delayed growth cessation across species. A positive correlation between LER and soil relative water content (SRWC) was observed. At the same SRWC, perennial grasses exhibited a higher LER with elevated [CO2], probably due to enhanced stomatal regulation. Despite stomatal closure and WSC accumulation, CO2 did not influence nighttime water potential or osmotic potential. The marked increase in leaf area across species resulted in similar (wheat and tall fescue) or higher (ryegrass) total water use by the end of the experiment, under both watered and unwatered conditions. Conclusions Elevated [CO2] mitigates the adverse effects of drought on leaf elongation in three Poaceae species, due to its impact on plant transpiration. Overall, these findings provide valuable insights into CO2 and drought interactions that may help anticipate plant responses to climate change. [ABSTRACT FROM AUTHOR]

The influence of biostimulant treatments on Passiflora species was assessed by observing changes in the growth and leaf functional compounds of plants exposed to humic acids (HAs) and seaweed extracts (SEs) to determine their optimal application doses. Four different concentrations of HA irrigation (0, 144, 192, and 285 mg·L-1 per pot assigned as HI-1, 2, 3, and 4, respectively), HA spraying (0, 96, 128, and 192 mg·L-1 as HS-1, 2, 3, and 4, respectively), SE irrigation (SI-1~4 at 0, 1, 1.33, and 2 g·L21 per pot, respectively), and SE spraying (SS-1~4 at 0, 0.5, 0.67, and 1 g·L21, respectively) treatments were applied to 'Tainung No. 1' and Passiflora suberosa cultivars every week to study their responses in plant growth traits and metabolites. Results show that leaf dry weight (DW) and root DW of 'Tainung No. 1' plants under SS-3 and HI-4 treatments, respectively, were significantly increased compared with controls. Both HI-3 and HS-3 treatments remarkably increased vine length, fresh weight (FW), and DW of leaves and shoots in P. suberosa plants compared with controls. Furthermore, 'Tainung No. 1' and P. suberosa plants respectively subjected to HI-2 and SS-4 treatments contributed to developing biostimulant applications for obtaining higher total phenol and flavonoid content per plant. Both Passiflora species are recommended for treatment with SS-4 and SE to maximize orientin and isovitexin content, and for all their beneficial applications and sustainable use in agriculture. [ABSTRACT FROM AUTHOR]

A major challenge in biodiversity research is the incorporation of species interactions into frameworks describing population and community response to global edfnmental change (GEC). Mutualisms are a type of species interaction especially sensitive to changing environmental conditions, and the breakdown of facilitative species interactions could amplify the negative impacts of novel climate regimes on focal species.Here, we investigate how reciprocal interactions between two coastal foundation species, the eastern oyster (Crassostrea virginica) and eelgrass (Zostera marina) shift in sign and magnitude in response to ocean warming (+1.5°C) and acidification (−0.4 pH) via a manipulative co‐culture experiment in mesocosms.Under ambient environmental conditions, oysters facilitated eelgrass leaf growth and clonal reproduction by 35% and 38%, respectively. Simultaneously, eelgrass decreased the oyster condition index (the ratio of tissue to shell biomass) by 35%, indicating greater allocation of energy to shell growth instead of soft tissues at ambient conditions. Varying sensitivities of each species to ocean warming and/or acidification treatments led to complex shifts in species interactions that were trait dependent. As such, community outcomes under future conditions were influenced by species interactions that amplified and mitigated species response to environmental change.Synthesis: Given that species interaction effect sizes were similar in magnitude to effect sizes of warming or pH treatments, our results underscore the need to identify key species and interaction types that strongly influence community response to GEC. Specifically, for macrophyte‐bivalve interactions, understanding how physiological limitations on growth are impacted by environmental heterogeneity and co‐culture will support the successful restoration of natural populations and the rapid expansion of aquaculture. [ABSTRACT FROM AUTHOR]

Nitrogen (N) is an essential element for plant growth, development, and metabolism. In apple production, the excessive use of N fertilizer may cause high N stress. Whether high N stress can be alleviated by regulating melatonin supply is unclear. The effects of melatonin on root morphology, antioxidant enzyme activity and 13C and 15N accumulation in apple rootstock M9T337 treated with high N were studied by soil culture. The results showed that correctly raising the melatonin supply level is helpful to root development of M9T337 rootstock under severe N stress. Compared with HN treatment, HN+MT treatment increased root and leaf growth by 11.38%, and 28.01%, respectively. Under high N conditions, appropriately increasing melatonin level can activate antioxidant enzyme activity, reduce lipid peroxidation in roots, protect root structural integrity, promote the transport of sorbitol and sucrose to roots, and promote further degradation and utilization of sorbitol and sucrose in roots, which is conducive to the accumulation of photosynthetic products, thereby reducing the inhibitory effect of high N treatment on root growth. Based on the above research results, we found that under high N stress, melatonin significantly promotes nitrate absorption, enhances N metabolism enzyme activity, and upregulates related gene expression, and regulate N uptake and utilization in the M9T337 rootstock. These results presented a fresh notion for improving N application and preserving carbon-nitrogen balance. [ABSTRACT FROM AUTHOR]

ABSTRACT Drought is one of the most devastating causes of yield losses in crops like maize, and the anticipated increases in severity and duration of drought spells due to climate change pose an imminent threat to agricultural productivity. To understand the drought response, phenotypic and molecular studies are typically performed at a given time point after drought onset, representing a steady‐state adaptation response. Because growth is a dynamic process, we monitored the drought response with high temporal resolution and examined cellular and transcriptomic changes after rehydration at 4 and 6 days after leaf four appearance. These data showed that division zone activity is a determinant for full organ growth recovery upon rehydration. Moreover, a prolonged maintenance of cell division by the ectopic expression of PLASTOCHRON1 extends the ability to resume growth after rehydration. The transcriptome analysis indicated that GROWTH‐REGULATING FACTORS (GRFs) affect leaf growth by impacting cell division duration, which was confirmed by a prolonged recovery potential of the GRF1‐overexpression line after rehydration. Finally, we used a multiplex genome editing approach to evaluate the most promising differentially expressed genes from the transcriptome study and as such narrowed down the gene space from 40 to seven genes for future functional characterization. [ABSTRACT FROM AUTHOR]

This study determined production performance, nutrient intake, digestibility, and carcass traits from ostrich chicks fed with barberry (Berberis Vulgaris) leaves (BVL) as a replacement for alfalfa hay (AH). For 150 days, 30 male ostrich (Struthio camelus) (9.10 ± 0.89 kg live weight, mean ± SD) were randomly grouped into five dietary BVL inclusion levels to replace AH: a control diet based on 100% AH (CTRL), and four groups with BVL as a substitute for AH at varying levels including 25% (BVL25), 50% (BVL50), 75% (BVL75), and 100% (BVL100). The average daily feed intakes (ADFI) were recorded and birds were weighed monthly. The nutrient intake and digestibility were measured for the following final 5 days of the trial. The ADFI was higher (p = 0.025) for BVL100 and BVL75 than for CTRL. Ostriches fed on BVL50 had the highest average daily gain (ADG, p = 0.025) and the lowest feed conversation ratio (FCR, p = 0.0001). Return per kg BW gain was also enhanced (p = 0.018) with BVL50 feeding. Dietary BVL levels did not affect the digestibility of OM (p = 0.257), CP (p = 0.260), EE (p = 0.610), CF (p = 0.427), and Ash (p = 0.461). Also, there were no changes in AME (p = 0.180) or AMEn (p = 0.670). The hot carcass weight (HCW, p = 0.331) and dressing BW (p = 0.237) were not affected. Overall, the results suggest that BVL can replace AH in ostrich diets without deleterious impact on performance, however, its partial substitution (for 50% AH of diet) has been associated with improved ADG, FCR, and reduced feeding costs. [ABSTRACT FROM AUTHOR]

In this study, the effects of supplemental or night interruptional blue light (S-BL or NI-BL) positioning on morphological growth, photoperiodic flowering, and expression of floral genes in Chrysanthemum morifolium were investigated. Blue light-emitting diodes (LEDs) at an intensity of 30 μmol·m−2·s−1 photosynthetic photon flux density (PPFD) were used for 4 h either (1) to supplement the white LEDs at the end of the 10 h short-day (SD10 + S-BL4) and 13 h long-day conditions (LD13 + S-BL4), or (2) to provide night interruption in the SD10 (SD10 + NI-BL4) and LD13 (LD13 + NI-BL4). The S-BL4 or NI-BL4 was positioned to illuminate either the shoot tip, the youngest leaf (vigorously growing the third leaf from the shoot tip), or the old leaf (the third leaf from the stem base). In the text, they will be denoted as follows: SD10 + S-BL4-S, -Y, or -O; SD10 + NI-BL4-S, -Y, or -O; LD13 + S-BL4-S, -Y, or -O; LD13 + NI-BL4-S, -Y, or -O. Normally, the LD13 conditions enhanced more vegetative growth than the SD10 periods. The growth of leaves, stems, and branches strongly responded to the S-BL4 or NI-BL4 when it was targeted onto the shoot tip, followed by the youngest leaf. The SD10 + S-BL4 or +NI-BL4 on the old leaf obviously suppressed plant extension growth, resulting in the smallest plant height. Under LD13 conditions, the flowering-related traits were significantly affected when the S-BL4 or NI-BL4 was shed onto the youngest leaf. However, these differences do not exist in the SD10 environments. At the harvest stage, other than the non-flowered LD13 treatment, the LD13 + S-BL4 irradiating the youngest leaf induced the most flowers, followed by the shoot tip and old leaf. Moreover, LD13 + NI-BL4 resulted in the latest flowering, especially when applied to the shoot tip and old leaf. However, the SD10 + S-BL4 or + NI-BL4 irradiated the shoot tip, youngest leaf, or old leaf all significantly earlier and increased flowering compared to the SD10 treatment. Overall: (1) Generally, vegetative growth was more sensitive to photoperiod rather than lighting position, while, during the same photoperiod, the promotion of growth was stronger when the light position of S-BL4 or NI-BL4 was applied to the shoot tip or the youngest leaf. (2) The photoperiodic flowering of these short-day plants (SDPs) comprehensively responded to the photoperiod combined with blue light positioning. Peculiarly, when they were exposed to the LD13 flowering-inhibited environments, the S-BL4 or NI-BL4 shed onto the leaves, especially the youngest leaves, significantly affecting flowering. [ABSTRACT FROM AUTHOR]

The polyketide antibiotic 2,4-diacetylphloroglucinol (DAPG) is a phenolic secondary metabolite produced by Pseudomonas spp. that serves as a biocontrol agent. DAPG is known to inhibit root growth and seed germination in plants. However, the effects of DAPG on leaf growth have not been elucidated yet. In this study, we found that applying DAPG at concentration above 30 µM suppresses the development of Arabidopsis leaves. Using an integrated proteomic analysis approach, we investigated the physio-molecular responses of Arabidopsis leaves under 10–100 µM DAPG treatment. Two-dimensional electrophoresis (2-DE)-based proteomic analysis identified 46 differentially expressed spots with at least a twofold change, corresponding to 37 proteins involved in stress and defense, metabolism, photosynthesis, and carbohydrate metabolism processes. Furthermore, we found that the expressions of stress and defense-related proteins were upregulated in response to DAPG, while those of photosynthesis-related proteins were downregulated. Additionally, RT-PCR result showed that the identified DAPG-responsive proteins in the proteomic analysis were similarly regulated at the transcriptional level. Collectively, our findings provide insights into the molecular mechanism of the response to DAPG in Arabidopsis leaves. [ABSTRACT FROM AUTHOR]

The study of chlorophyll fluorescence parameters is very important for understanding plant photosynthesis. Monitoring cotton chlorophyll fluorescence parameters via spectral technology can aid in understanding the photosynthesis, growth, and stress of cotton fields in real time and provide support for cotton growth regulation and planting management. In this study, cotton plot experiments with different water treatments were set up to obtain the spectral reflectance of the cotton canopy, the maximum photochemical quantum yield (Fv/Fm), and the photochemical quenching coefficient (qP) of leaves at different growth stages. Support vector machine regression (SVR), random forest regression (RFR), and artificial neural network regression (ANNR) were used to establish a fluorescence parameter inversion model of the cotton canopy leaves. The results show that the original spectrum was transformed by multivariate scattering correction (MSC), the standard normal variable (SNV), and continuous wavelet transform (CWT), and the model constructed with Fv/Fm passed accuracy verification. The SNV-SVR model at the budding stage, the MSC-SVR model at the early flowering stage, the SNV-SVR model at the full flowering stage, the MSC-SVR model at the flowering stage, and the CWT-SVR model at the full boll stage had the highest estimation accuracy. The accuracies of the three spectral preprocessing and qP models were verified, and the MSC-SVR model at the budding stage, SNV-SVR model at the early flowering stage, MSC-SVR model at the full flowering stage, SNV-SVR model at the flowering stage, and CWT-SVR model at the full boll stage presented the highest estimation accuracies. [ABSTRACT FROM AUTHOR]

Leaves are the primary harvest portion in forage crops such as alfalfa (Medicago sativa). Delaying leaf senescence is an effective strategy to improve forage biomass production and quality. In this study, we employed transcriptome sequencing to analyze the transcriptional changes and identify key senescence-associated genes under age-dependent leaf senescence in Medicago truncatula, a legume forage model plant. Through comparing the obtained expression data at different time points, we obtained 1057 differentially expressed genes, with 108 consistently up-regulated genes across leaf growth and senescence. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses showed that the 108 SAGs mainly related to protein processing, nitrogen metabolism, amino acid metabolism, RNA degradation and plant hormone signal transduction. Among the 108 SAGs, seven transcription factors were identified in which a novel bZIP transcription factor MtbZIP60 was proved to inhibit leaf senescence. MtbZIP60 encodes a nuclear-localized protein and possesses transactivation activity. Further study demonstrated MtbZIP60 could associate with MtWRKY40, both of which exhibited an up-regulated expression pattern during leaf senescence, indicating their crucial roles in the regulation of leaf senescence. Our findings help elucidate the molecular mechanisms of leaf senescence in M. truncatula and provide candidates for the genetic improvement of forage crops, with a focus on regulating leaf senescence. [ABSTRACT FROM AUTHOR]

Tiller buds of grasses like sorghum form in close contact with the overlying leaf. A prior study showed that tiller bud growth was repressed by mechanical signals generated by contact with the leaf, and that removing the leaf permitted robust bud growth. The current study was conducted to better define the rapid changes in bud growth following leaf removal, and to identify potential hormones and pathways associated with the phenomenon. Time course image analyses showed that bud growth was promoted within 6 h of leaf removal. This rapid growth response was associated with increased JA within 2.5 min and with massive reprogramming of the bud transcriptome within 10 min. A role for ERF transcription factors was supported by overrepresentation of differentially expressed ERF genes in the bud transcriptome data, and by the discovery of an ERF promoter motif in highly induced genes. This study provides insights into both the pathways associated with mechanical signaling, and with pathways involved in coordinating the very rapid hormonal and transcriptional changes underlying tiller bud growth. These findings may suggest targets to improve crop resiliency to mechanical stresses (ie- lodging) and to manipulate tiller development for enhanced productivity or end-use. [ABSTRACT FROM AUTHOR]

Ethephon (2-chloroethylphosphonic acid) is frequently used for flush management in order to maximize flowering in litchi. However, the optimal dosage of ethephon, which balances between flush control effect and the detrimental effect on leaves, is unknown. This study aimed to identify the optimal ethephon dosage and test more efficient ethephon application methods, using a drone for flush control and flowering promotion in litchi. The effects of a single manual full-tree spray of 250, 500 or 1000 mg/L of ethephon in early November on the bud break rate, leaf drop rate, net photosynthetic rate, LcFT1 expression and floral induction (panicle emergence rate and panicle number) in 'Jingganghongnuo' litchi were examined in the season of 2021–2022. In the season of 2022–2023, the effects of drone application of 1000 mg/L of ethephon in early November on bud growth and floral induction were observed. The results showed that the manual ethephon treatments were effective at enforcing bud dormancy and elongating the dormancy period and that the effects were positively dependent on dosage. One manual spray of 1000 mg/L of ethephon in late autumn enabled a dormancy period of 6 weeks. The treatments advanced seasonal abscission of old leaves in winter and caused short-term suppression on photosynthesis within 2 weeks after treatment. Ethephon treatments, especially at 1000 mg/L, enhanced the expression of LcFT1 in the mature leaves and promoted floral induction reflected by earlier panicle emergence and increased panicle emergence rate and number in the terminal shoot. The floral promotion effect was also positively dosage dependent. The cumulative chilling hours below 15 °C from the date of treatment to the occurrence of a 20% panicle emergence rate were lowered in ethephon treatments. A drone spray of 1000 mg/L of ethephon solution consumed a sixth of the manual spray solution volume and was two thirds less effective in suppressing bud break compared with manual spraying. However, it achieved a significant flowering promotion effect comparable to traditional manual spraying. The results suggest that ethephon application enhanced flowering responsiveness to chilling as well as enforced bud dormancy. The application of ethephon with a drone proved to be an efficient method for flush control and flower promotion. [ABSTRACT FROM AUTHOR]

The hydrogen isotopic compositions of leaf‐wax n‐alkanes (δDalk) and n‐alkanoic acids (δDacid) reflect ambient climatic conditions (including precipitation δD values, δDprecip). However, the understanding of climatic conditions of exactly which period (i.e., early or entire period of the leaf's lifespan) these biomarkers preserve is still evolving. Previous studies on the δDalk and δDacid values, done only in extra‐tropical regions, mostly indicate that δDalk values are biased toward the early growing season whereas δDacid values are not biased toward any season. To decipher the seasonal bias in δDalk and δDacid records from tropics, we conducted a long‐duration experiment wherein deciduous and evergreen species were grown using normal water (δD = −2‰) during early stages of the leaf growth and later using isotopically labeled water (δD = 1,000‰). Our experiment revealed (a) in deciduous and evergreen species, δDalk and δDacid values reflect δDprecip during early stages of the leaf growth, (b) synchronous synthesis of n‐alkanes and n‐alkanoic acids, and (c) in deciduous species, minor incorporation of the previous year's photosynthates in leaf wax pool of the current year's mature leaves. Our study suggests that δDalk and δDacid records in the tropics are biased toward the climatic conditions prevailing during early stages of leaf growth. This bias should be considered while comparing the δDprecip values generated from leaf wax proxy records and isotope‐enabled atmospheric circulation models. Plain Language Summary: The hydrogen isotopic composition of leaf wax n‐alkanes and n‐alkanoic acids preserved in sediments have been used extensively for reconstructing past climate variability. However, whether these records reflect a climate of a specific season or an entire year is not known. In our experiment, we grew various tropical plants using normal water during early growing season and heavy water during later growing season. Lack of isotopic signal associated with the heavy water in leaf wax compounds suggested that the bulk of leaf wax production occurred during the early growing season of the leaves. As the hydrogen isotopic composition of the leaf wax compounds reflects ambient climatic conditions, our results suggested that the hydrogen isotope record of tropical leaf wax compounds reflects the climatic conditions prevailing during the early stages of leaf growth. Key Points: Hydrogen isotope records of leaf wax n‐alkane and n‐alkanoic acid from the tropics reflect climatic conditions during the early leaf growthBoth deciduous and evergreen leaf wax hydrogen isotope records are biased toward the early stages of the leaf growthThis bias should be considered while comparing the hydrogen isotope of precipitation generated from climate models and leaf wax records [ABSTRACT FROM AUTHOR]

Nursery material intended for establishing intensive apple orchards should be characterised by a dominant and straight leader with an appropriate number of shoots that develop at the right height and are regularly spaced along the leader. The use of well-branched trees can lead to fruiting in the first year after planting. However, many apple varieties have difficulty forming lateral shoots due to strong apical dominance. The aim of the study was to assess the effectiveness of treatments stimulating the branching of maiden apple trees of the 'Gloster' variety. The research was carried out in 2017–2019 at a private nursery farm located in eastern Poland. The studied trees were subjected to a mechanical branching stimulation treatment, which consisted of pinching off 4–5 of the youngest leaves located below the growth cone, and chemical branching stimulation treatments, which consisted of applying growth regulator mixtures in the form of an aqueous solution, i.e., BA+GA3 and BA+GA4+7. The conducted studies showed that the type of branching-stimulating treatment had a significant effect on the height and trunk diameter of the maiden trees, the number of lateral shoots, the average length of one shoot and the sum of the lengths of all sylleptic shoots. The maiden trees treated with BA+GA3 were characterised by the best quality among the analysed combinations. Maiden apple trees treated with BA+GA3 were the tallest (2017—167.7 cm; 2018—175.3 cm; 2019—164.4 cm), produced the largest number of shoots (2017—6.5 pcs; 2018—6.8 pcs; 2019—6.3 pcs) and had the largest sum of lateral shoot lengths (2017—148.0 cm; 2018—155.4 cm; 2019—140.6 cm) among the evaluated combinations. The number of treatments and the concentration of applied growth regulators had a significant effect on the structure of the crown of the maiden apple trees of the 'Gloster' cultivar. [ABSTRACT FROM AUTHOR]

Elucidating plant functions and identifying crop productivity bottlenecks requires the accurate quantification of their performance. This task has been attained through photosynthetic models. However, their traditional focus on the leaf's capacity to uptake CO2 is becoming increasingly restrictive. Advanced bioengineering of C3 plants has made it possible to increase rates of CO2 assimilation by packing photosynthetic structures more densely within leaves. The operation of mechanisms that concentrate CO2 inside leaves can boost rates of assimilation while requiring a lower investment in carboxylating enzymes. Therefore, whether in the context of spontaneous plants or modern manipulation, considering trade‐offs in resource utilization efficiency emerges as a critical necessity. I've developed a concise and versatile analytical model that simulates concurrent leaf and root growth by balancing instantaneous fluxes of carbon and nitrogen. Carbon is made available by leaf photosynthesis, encompassing all types of biochemistries, while nitrogen is either taken up by roots or remobilized after senescence. The allocation of leaf nitrogen between light or carbon reactions was determined using a fitting algorithm: growth maximisation was the only reliable fitting goal. Both the leaf nitrogen pool and the root‐to‐leaf ratio responded realistically to various environmental drivers (CO2 concentration, light intensity, soil nitrogen), replicating trends typically observed in plants. Furthermore, modifying the strength of CO2 concentrating mechanisms proved sufficient to alter the root‐to‐leaf ratio between C3 and C4 types. This direct and mechanistic one‐to‐one link convincingly demonstrates, for the first time, the functional dependence of a morphological trait on a single biochemical property. [ABSTRACT FROM AUTHOR]

Paphiopedilum primulinum and Paphiopedilum glaucophyllum have unique labellum colour and shaped like lady's slippers. These orchids are from the Cochlopetalum section, which is exclusively found in Sumatra and Java. There are so many people that desire to collect these plants illegally. Due to extensive commercial exploitation, Paphiopedilum is in danger of going extinct. Tissue culture techniques are utilised to conserve threatened orchid germplasm in a short time. The success of the in vitro culture depends on the accuracy of the basic media composition used. The Ambon Lumut banana (ALB) can accelerate plant growth and cell division. Banana added to the culture medium was prepared by mashing the ripe flesh (3.5 months old) using a mortar. This research aims to investigate the effect of banana homogenate supplemented media for the orchids P. primulinum and P. glaucophyllum based on the parameters of difference of plant height (calculated from the base of the stem to the tip of the plant stem), number of leaves, and number of roots. The measurement method was carried out using a ruler with a centimetre scale. Observations and documentation were carried out once a week for 7 weeks after planting (WAP) for P. primulinum and P. glaucophyllum. The results showed that V Murashige and Skoog (MS) + ALB homogenate is a better medium for P. primulinum and P. glaucophyllum growth than media without banana homogenate. The highest values of plant height, leaf growth and root growth of P. primulinum with banana homogenate were 0.44 cm, 0.63 leaves, and 0.50 roots, respectively. The highest values of plant height and leaf growth of P. glaucophyllum were 0.75 cm and 1.90 leaves, respectively. Culture medium added banana homogenate was able to support the propagation of plants, some of which are returned to nature and others used for industrial purposes (conventionally cultivated by the community). [ABSTRACT FROM AUTHOR]

Catharanthus roseus (C. roseus) can produce over 150 types of monoterpenoid indole alkaloids (MIAs), including vinblastine and vincristine, which are currently the primary sources of these alkaloids. Exploring the complex regulatory mechanisms of C. roseus is significant for resolving MIA biosynthesis. The Lateral Organ Boundaries Domain (LBD) is a plant-specific transcription factor family that plays crucial roles in the physiological processes of plant growth, stress tolerance, and specialized metabolism. However, the LBD gene family has not been extensively characterized in C. roseus, and whether its members are involved in MIA biosynthesis is still being determined. A total of 34 C. roseus LBD (CrLBD) genes were identified. RNA-Seq data were investigated to examine the expression patterns of CrLBD genes in various tissues and methyl jasmonate (MeJA) treatments. The results revealed that the Class Ia member CrLBD4 is positively correlated with iridoid biosynthetic genes (p < 0.05, r ≥ 0.8); the Class IIb member CrLBD11 is negatively correlated with iridoid biosynthetic genes (p < 0.05, r ≤ −0.8). Further validation in leaves at different growth stages of C. roseus showed that CrLBD4 and CrLBD11 exhibited different potential expression trends with iridoid biosynthetic genes and the accumulation of vindoline and catharanthine. Yeast one-hybrid (Y1H) and subcellular localization assays demonstrated that CrLBD4 and CrLBD11 could bind to the "aattatTCCGGccgc" cis-element and localize to the nucleus. These findings suggest that CrLBD4 and CrLBD11 may be potential candidates for regulating MIA biosynthesis in C. roseus. In this study, we systematically analyzed the CrLBD gene family and provided insights into the roles of certain CrLBDs in the MIA biosynthesis of C. roseus. [ABSTRACT FROM AUTHOR]

Silicon is crucial in the process of absorbing and transporting nutrients to the plants, which in turn helps to decrease the toxicity of iron (Fe), aluminium (Al), and manganese (Mn). Plants can better endure drought and salt stress because it strengthens their tissues. Seaweed extracts serve as biostimulants and are considered vital nutrients for sustainable agriculture due to their organic nature and ability to decompose naturally. The present study aimed to compare the nutritional content and growth rate of papaya cv. Red Lady after being treated with silicon and seaweed extract. The papaya plants were moved to new spots 1.5m x 1.5m apart. A Factorial Randomised Block Design with nine treatments and three replications was used for the field experiments. At 6, 7, 8, and 9 months after replanting, silicon (potassium silicate at 0.3% and 0.5%) and seaweed extract at 3% and 5% were made through foliar application. The study showed that the plant's height (278.66 cm), stem width (174.8 cm), number of leaves per plant (33.17), petiole length (127.3 cm), and leaf length (123 cm) were all higher in T8 (SE 5%+PS 0.5%) than in T7 (SE 5%+PS 0.3%) or T5 (SE 3%+PS 0.5%). A study of leaf minerals in synchrony showed that the highest levels of N, P, K, S, Ca, and Mg were found in the leaves nine months after the crop was transplanted. Thus, using modest concentrations of silicon and seaweed extracts topically enhances growth, nutritional content in leaves, and resistance to biotic and abiotic stress, as well as Papaya Ring Spot Virus (PRSV), collar rot, and anthracnose. [ABSTRACT FROM AUTHOR]

Rice is one of the major grain crops in the world. It is of great significance to accurately inverse the chlorophyll content during growth and development in the field. However, there are some deficiencies using conventional interpretability that are driven by data inversion of the physical and chemical parameters. The leaf radiative transfer model can be expected to effectively simulate the spectral information of rice leaves, and then describe the effects of leaf parameters on spectral reflectance. A strong mechanism model can be used for the inversion of chlorophyll content of rice leaves, according to the physically driven method. The PIOSL (PROSPECT considering the internal optical structure of the leaves) model can be utilized to assume that the internal structure of the leaf blade is composed of the superposition of two layers of optical properties, which is much more in line with the actual growth of the plant. This study aims to verify the feasibility of retrieving rice leaf chlorophyll content by the PIOSL model. Firstly, the sensitivity analysis was carried out on each input parameter into the PIOSL model. The parameters Cab and Cab12 were determined as the high sensitivity in the 400-750 nm band. The inversion of chlorophyll content (Cab) was then implemented. The projection (SPA) was used to extract the features of spectral data. Five characteristic bands were sensitive to chlorophyll: 490, 575, 645, 675, and 725 nm. A multiple regression model was constructed to realize the inversion of rice leaf chlorophyll. The inversion performance of the mechanism model was then evaluated using the PIOSL model. The chlorophyll content of rice leaves was also inverted to construct an initial lookup table. LSE (least squares estimate) was used to screen the simulated sample data in the initial lookup table closer to the measured spectra. A classification prediction model was constructed using a support vector machine (SVM), in order to determine whether the combination of the screened sample parameters was in line with the actual growth of the leaves. The improved lookup table was randomly split into the training and test sets in the ratio of 7:3. The hierarchical inversion of chlorophyll content of rice leaves was performed to construct the WOA-ELM (whale optimization, WOA; extreme learning machine, ELM) inversion model. A comparison was made on the commonly-used PROSPECT model with the same data. An improved lookup table was then obtained using the PROSPECT model. The WOA-ELM model was used for the chlorophyll inversion. The results showed that the R² and RMSE of the inversion model using PIOSL-WOA-ELM were 0.977 and 2.356 μg/cm2, respectively, and the inversion accuracies of both models were above 0.9, compared with the PROSPECT-WOA-ELM model. According to the numerically driven inversion model and the physically-driven radiative transfer model, the inversion accuracy of the PIOSLWOA-ELM model was higher than that of the multivariate regression with continuous projection. Therefore, it is feasible for a more effective inversion with the radiative transfer mechanism. The finding can provide new ideas for the accurate inversion of the distribution of rice chlorophyll in the leaf, further effectively retrieving the crop physicochemical parameters. [ABSTRACT FROM AUTHOR]

Penicillium citrinum is one of the most prevalent tobacco spoilage fungi. However, the mechanisms underlying fungal growth on tobacco leaves remain largely unknown. In this study, transcriptomic analyses were performed to reveal the genome-wide expression profiles of P. citrinum growing on tobacco leaves. First, a comparative analysis was conducted between two sets of transcriptomic data from P. citrinum growing on chemically defined media and tobacco leaves. Enrichment analyses showed that differentially regulated genes were mainly associated with carbohydrate degradation (e.g., cellulose, pectin, and xylan) and the catabolism of fatty acids and aromatic compounds. Comparative transcriptomic analyses between different time points indicated that the fungal transcriptome varied dynamically during the spoilage process, and the enriched terms were associated with small-molecule degradation and fungal development. Enrichment analyses indicated that more up-regulated genes appeared in all enriched Gene Ontology terms. Notably, more organelles significantly contributed to further fungal growth on tobacco leaves. In conclusion, P. citrinum activates a comprehensive transcriptome that changes dynamically when causing tobacco mildew. [ABSTRACT FROM AUTHOR]

BACKGROUND: Bamboo leaf flavonoids (BLF) are the main bioactive ingredients in bamboo leaves. They have antioxidant, anti‐inflammatory, antibacterial, and other effects. In this study, the effects of dietary BLF on growth performance, immune response, antioxidant capacity, and intestinal microbiota of broilers were investigated. A total of 288 broilers were divided into three groups with eight replicates and 12 birds in each replicate. Broilers were fed a basic diet or the basic diet supplemented with 1000 or 2000 mg kg−1 BLF for 56 days. RESULTS: The results showed that supplementation of BLF increased body weight (BW) and average daily weight gain (ADG), and reduced average daily feed intake (ADFI) (P < 0.05). The serum immunoglobulin A (IgA), immunoglobulin M (IgM), and interleukin 10 (IL‐10) content of broilers in the BLF1000 group was increased and the interleukin 1β (IL‐1β) and tumor necrosis factor‐α (TNF‐α) content was decreased (P < 0.05). The levels of IgM and IL‐10 in jejunum mucosa were found to be enhanced by BLF (P < 0.05). The BLF1000 group exhibited a significant reduction in the concentration of TNF‐α (P < 0.05). Serum and jejunum mucosa total antioxidant capacity (T‐AOC) levels in the BLF1000 group were increased (P < 0.05). The serum catalase (CAT) and glutathione peroxidase (GSH‐Px) effects of the BLF1000 group and serum CAT effects of BLF2000 group were increased (P < 0.05). The CON group demonstrated a lower relative abundance of Christensenellaceae_R‐7_group and Oscillibacter than the BLF group (P < 0.05). CONCLUSION: Dietary BLF inclusion enhanced the growth performance, immune, and antioxidant functions, improved the intestinal morphology, and ameliorated the intestinal microflora structure in broiler. Adding 1000 mg kg−1 BLF to the broiler diet can be considered as an effective growth promoter. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

In open fields, plants experience dynamic changes in environment, particularly radiation, temperature, wind, and humidity but their short-term responses have not been adequately characterized under natural conditions. In this study, we assessed causal effects of rapid radiation fluctuation on seven plant parameters in open fields of cotton and sweet corn canopies. The parameters are evapotranspiration (ET), canopy photosynthesis indicated by CO2 flux from air to canopy (FCO2), sensible heat energy flux (H), canopy conductance for water vapor (gcw), canopy surface temperature (Ts), shoot and leaf elongation rate, and stem diameter change. The energy and CO2 fluxes were measured with Bowen ratio/energy balance/CO2 gradient (BREB+) technique, using averaging time of 5 min. Shoot + leaf elongation and stem diameter change were monitored with position transducers using averaging time of 1 min. All parameters were all found to respond to change in radiation and transpiration within minutes or sooner. While radiation effects on canopy gas exchanges are expected, illuminating are the indirect but immediate effects on shoot + leaf growth and stem diameter change through radiation effects on transpiration and plant water status. A novel finding is that gcw also responded within minutes or sooner to radiation fluctuations and that FCO2 was related almost linearly to gcw. Results are discussed in terms of soil-plant-atmosphere continuum, and interpreted in terms of dynamic interactions between transpiration and plant water status. The clear inverse relationship between ET and elongation rate or stem diameter changes provides additional evidence supporting the validity of 5-min averaging for the BREB + technique. [ABSTRACT FROM AUTHOR]

Productivity in strawberry is dependent on carbon assimilation in the leaves. The main scenarios for climate change include an increase in the concentration of CO2 in the atmosphere and an increase in temperature. This review examined the relationship between leaf growth and temperature in strawberry. Leaf growth follows a linear, sigmoid or Gaussian pattern over the season or is variable depending on the weather. There was a linear increase in leaf growth with temperatures below 26°C, and a linear decrease in growth with temperatures above 26°C. In other studies, the mean lower optimum for 90% of maximum growth was 18.3° ± 3.9°C, the median was 19.6°C, and the range was from 7.2° to 22.0°C (N = 11). The mean higher optimum was 27.3° ± 3.6°C, the median was 27.4°C, and the range was from 21.1° to 33.5°C (N = 12). Leaf growth was higher under 30°C and lower above 30°C. Limited studies suggest that elevated CO2 will not counteract the impact of high temperatures on growth. Climate change will increase leaf growth in cool areas and decrease growth in warm areas. The decrease in leaf growth in locations with temperatures above 30°C will contribute to lower yields under global warming. [ABSTRACT FROM AUTHOR]

Pinus yunnanensis (Franch), a species endemic to southwestern China, provides significant ecological and economic benefits. The quality of afforestation can be enhanced by promoting high-quality sprout growth. This study analyzed the effects of six fertilization treatments following top pruning: T1 (N: 0 g/plant−1; P: 0 g/plant−1), T2 (N: 0 g/plant−1; P: 2 g/plant−1), T3 (N: 0 g/plant−1; P: 4 g/plant−1), T4 (N: 0.6 g/plant−1; P: 0 g/plant−1), T5 (N: 0.6 g/plant−1; P: 2 g/plant−1), and T6 (N: 0.6 g/plant−1; P: 4 g/plant−1). The accumulation and allocation of aboveground biomass in roots, stems, and leaves of P. yunnanensis were measured, as well as changes in biomass per plant at 90 days (early stage), 180 days (middle stage), and 270 days (late stage) post-fertilization. At 90 days, root biomass accumulation in T3 was significantly higher, by 13.31%, compared to that in T1 (p < 0.05). The growth rates of stem and plant biomass followed the order T6 > T1 > T3 > T5 > T4 > T2. The biomass of sprouts and individual plants exhibited allometric growth under T1, T5, and T6 treatments. At 180 days, needle biomass allocation in T1 and T4 increased by 7.47% and 8.6%, respectively, compared to 90 days. Combined nitrogen–phosphorus fertilization significantly influenced aboveground biomass allocation, promoting growth more effectively than other treatments. By 270 days, the stem and individual biomass in T2 and T3 treatments showed significant differences (p < 0.01) compared to other treatments. Overall, root, stem, and sprouts were primarily influenced by phosphorus fertilization, while nitrogen fertilization notably promoted needle and leaf growth in later stages. The aboveground components were more affected by phosphorus fertilization. The combination of nitrogen and phosphorus fertilizers enhanced early-stage stem and sprouts, as well as late-stage root development. [ABSTRACT FROM AUTHOR]

Plant wearable sensors have shown exceptional promise in continuously monitoring plant health. However, the potential adverse effects of these sensors on plant growth remain unclear. This study systematically quantifies wearable sensors' interference with plant growth using two ornamental species, Peperomia tetraphylla and Epipremnum aureum. We evaluated the impacts of four common disturbances—mechanical pressure, hindrance of gas exchange, hindrance of light acquisition, and mechanical constraint—on leaf growth. Our results indicated that the combination of light hindrance and mechanical constraint demonstrated the most significant interference. When the sensor weight was no greater than 0.6 g and the coverage was no greater than 5% of the leaf area, these four disturbances resulted in slight impacts on leaf growth. Additionally, we fabricated a minimally interfering wearable sensor capable of measuring the air temperature of the microclimate of the plant while maintaining plant growth. This research provides valuable insights into optimizing plant wearable sensors, balancing functionality with minimal plant interference. [ABSTRACT FROM AUTHOR]

Al3+-toxicity in acidic soils is among the main abiotic stress factors that generate adverse effects in plant growth; in leaves, it affects several physiological parameters such as photosynthesis and ROS balance, leading to limited crop production. On the other hand, sulfur is a macronutrient that has a key role against oxidative stress and improves plant growth in acidic soils; however, the implication of sulfate nutritional status in the modulation of short-term Al3+-toxicity tolerance mechanisms in plant leaves are barely reported. This study is focused on the role of sulfate on the leaf response of an Al3-sensitive perennial ryegrass (Lolium perenne cv. Jumbo) after 48 h of exposure. Lolium perenne cv. Jumbo seeds were cultivated in hydroponic conditions with modified Taylor Foy solutions supplemented with 120, 240, and 360 μM sulfate in the presence or absence of Al3+-toxicity. The L. perenne cv. Jumbo leaves were collected after 48 h of Al3+-toxicity exposure and processed to evaluate the effects of sulfate on Al3+ toxicity, measuring total proteins, mineral uptake, photosynthesis modulation, and ROS defense mechanism activation. The plants exposed to Al3+-toxicity and cultivated with a 240 µM sulfate amendment showed a recovery of total proteins and Ca2+ and Mg2+ concentration levels and a reduction in TBARS, along with no changes in the chlorophyll A/B ratio, gene expression of proteins related to photosynthesis (Rubisco, ChlAbp, and Fered), or ROS defense mechanism (SOD, APX, GR, and CAT) as compared with their respective controls and the other sulfate conditions (120 and 360 µM). The present study demonstrates that adequate sulfate amendments have a key role in regulating the physiological response against the stress caused by Al3+ toxicity. [ABSTRACT FROM AUTHOR]

The 'RD6' glutinous rice (Oryza sativa L.), developed from the popular aromatic rice 'KDML105' through gamma irradiation, is a key economic cultivar widely consumed in Thailand and exported throughout Asia. However, cultivation of the 'RD6' faces challenges due to its low grain yield and growth performance. The objective of this study was to use biostimulant chitosan and brassinosteroids to promote growth performance, nutrition, and yield of the 'RD6' rice. Chitosan (2.5, 5.0, 7.5, and 10.0 mg L-1) and brassinosteroids (0.00005, 0.0005, 0.001, and 0.002 mg L-1) were sprayed twice at 40- and 75-d-old rice plants in paddy fields. The results demonstrated that foliar spraying with 7.5 mg L-1 chitosan can promote leaf growth of 'RD6' and improve grain yield by 24% as compared with the control. More pronounced effects were observed with 0.002 mg L-1 brassinosteroid, improving not only growth but also 'RD6' yield by 41.8% compared to the control. Moreover, 0.002 mg L-1 brassinosteroids can enhance antioxidant capacities (total phenolic contents), elemental K and Ca contents, and the aroma compound 2-acetyl-1-pyrroline (2AP) contents in the rice grains. Furthermore, the use of 0.002 mg L-1 brassinosteroids proved to be a financially sound investment, yielding superior net returns and an advantageous benefit-cost ratio compared with 7.5 mg L-1 chitosan. Therefore, the use of exogenous brassinosteroids on glutinous rice in this study offers promising advantages for promoting the growth and physiological performance of rice plants. [ABSTRACT FROM AUTHOR]

This study investigated the effects of Jatropha gossypiifolia leaf extract (JGE) on the performance and immune response of African sharptooth catfish. Fish (6.43 ± 0.26 g, n = 450, 30/replicate) were fed diets supplemented with 0, 0.5, 1.0, 1.5, or 2.0 g JGE/kg in triplicate for 84 days. Thereafter, the fish were injected with Aeromonas hydrophila and observed for 14 days. Fish fed 1.0–1.5 g JGE had higher (P < 0.05) weight gain, protein efficiency ratio, and villi absorption area, with highest performance at 1.0 g JGE. All the fish fed JGE-supplemented diets had enhanced (P < 0.05) activities of glutathione-s-transferase and glutathione peroxidase, compared to fish fed control diet, while the variations in erythrocyte and leucocyte counts were insignificant (P > 0.05). The post-challenge fish fed JGE-supplemented diets had higher lysozyme, phagocytic, and respiratory burst activities and survival than the control treatment. [ABSTRACT FROM AUTHOR]

Inner Mongolia, a major region in China for growing sugar beet, faces challenges caused by unscientific water and fertilizer management. This mismanagement restricts the improvement of sugar beet yield and quality and exacerbates water waste and environmental pollution. This study aims to evaluate the effects of reduced water and fertilizer on the growth and physiological metabolism of sugar beet taproot. Field experiments were conducted in Ulanqab, Inner Mongolia, in 2022 and 2023, using a split-plot design with three levels each of fertilization and irrigation. The study analyzed the effects of reduced water and fertilizer treatments on fresh taproot weight, respiration rate, energy metabolism, respiratory enzyme activity, and gene expression in sugar beet taproot. It was found that a 10% reduction in fertilizer significantly increased the beet taproot fresh weight. Further research revealed that during the rapid leaf growth phase and the taproot and sugar growth period, a 10% reduction in fertilizer upregulated HK and IDH gene expression and downregulated G6PDH gene expression in the beet taproot. This increased HK and IDH activities, decreased G6PDH activity, enhanced the activity of the EMP-TCA pathway, and inhibited the PPP. Taproot weight was positively correlated with the respiration rate, ATP content, EC, and ATPase, HK, and IDH activities, thereby increasing the taproot growth rate and taproot fresh weight, with an average increase of 4.0% over two years. These findings introduce a novel method for optimizing fertilizer use, particularly beneficial in water-scarce regions. Implementing this strategy could help farmers in western Inner Mongolia and similar areas improve crop yield and sustainability. This study offers new insights into resource-efficient agricultural practices, highlighting the importance of customized fertilization strategies tailored to local environmental conditions. [ABSTRACT FROM AUTHOR]

To investigate the dynamics of stomata, transpiration, and photosynthesis under varying light intensities and CO2 conditions during leaf development, the light response and CO2 response of stomatal conductance (gsw), transpiration rate (Tr), and net photosynthetic rate (Pn) were observed for rice leaves at different days after leaf emergence (DAE). The results showed that (1) as photosynthetically active radiation (PAR) increased, leaf gsw, Tr, and Pn initially increased rapidly and linearly, followed by a more gradual rise to maximum values, and then either stabilized or showed a declining trend. The maximum gsw, Tr, and Pn were smaller and occurred earlier for old leaves than for young leaves. The gsw, Tr, and Pn all exhibited a linear decreasing trend with increasing DAE, and the rate of decrease slowed down with the reduction in PAR; (2) as the CO2 concentration (Ca) increased, gsw and Tr decreased gradually to a stable minimum value, while Pn increased linearly and slowly up to the maximum and then kept stable or decreased. The gsw, Tr, and Pn values initially kept high and then decreased with the increase of DAE. These results contribute to understanding the dynamics in gsw, Tr, and Pn during rice leaf growth and their response to varied light and CO2 concentration conditions and provide mechanistic support to estimate dynamic evapotranspiration and net ecosystem productivity at field-scale and a larger scale in paddy field ecosystems through the upscaling of leaf-level stomatal conductance, transpiration, and photosynthesis. [ABSTRACT FROM AUTHOR]

This study was conducted to investigate both fruit and different levels of leaf supplementation on the growth performance, organ indices and intestinal microflora of rats. Twenty-five healthy male Sprague--Dawley rats were randomly divided into five groups. The rats in the control (NC) and positive control (PC) groups were fed by gavage a basal diet and a basal diet with 4 g/ kg of L. barbarum fruit homogenate, respectively. The test (LD, MD, and HD) groups were fed basal diets with additional 2, 4, and 8 g/kg of L. barbarum leaf homogenate, respectively. The feeding period was 35 d. The result revealed that the rats in the LD group had the highest average weight gain (p < 0.05). The cardiac and renal indexes in the LD and MD groups were significantly higher than in NC group, respectively (p < 0.05). Diversity analysis revealed that adding low concentrations of L. barbarum leaf homogenates markedly reduced the Shannon index of the rats cecum (p < 0.05). The relative abundance of Verrucomicrobiota was higher in the LD group than those in other groups (p < 0.05). The relative abundance of Actinobacteriota was found significantly higher in PC group than others (p < 0.05). The relative abundance of Akkermansia in LD group was the highest (p < 0.05). The relative abundance of Romboutsia in the PC group was considerably higher than that in other groups. The relative abundance of Candidatus_Saccharimonas in the supplementation groups was appreciably lower than those found in other groups. The relative abundance of Alloprevotella was significantly lower in PC, LD, and MD groups than in NC and HD groups (p < 0.05). The relative abundance of Oscillibacter was significantly higher in HD group than in other groups (p < 0.05). Thus, L. barbarum leaf homogenate fed to rats could increase their growth performance, internal organ weights and additionally enhance the relative abundance of beneficial bacteria. Therefore, based on the obtained data in the current study, a dose of L. barbarum leaf homogenate supplemented with 2 g/kg in diet is recommended, however, further studies are required to confirm, especially in animals. [ABSTRACT FROM AUTHOR]

Foliar development involves successive phases of cell proliferation and expansion that determine the final leaf size, and is characterized by an early burst of reactive oxygen species generated in the photosynthetic electron transport chain (PETC). Introduction of the alternative PETC acceptor flavodoxin in tobacco chloroplasts led to a reduction in leaf size associated to lower cell expansion, without affecting cell number per leaf. Proteomic analysis showed that the biogenesis of the PETC proceeded stepwise in wild-type leaves, with accumulation of light-harvesting proteins preceding that of electron transport components, which might explain the increased energy and electron transfer to oxygen and reactive oxygen species build-up at this stage. Flavodoxin expression did not affect biogenesis of the PETC but prevented hydroperoxide formation through its function as electron sink. Mature leaves from flavodoxin-expressing plants were shown to contain higher levels of transcripts encoding components of the proteasome, a key negative modulator of organ size. Proteome profiling revealed that this differential accumulation was initiated during expansion and led to increased proteasomal activity, whereas a proteasome inhibitor reverted the flavodoxin-dependent size phenotype. Cells expressing plastid-targeted flavodoxin displayed lower endoreduplication, also associated to decreased organ size. These results provide novel insights into the regulation of leaf growth by chloroplast-generated redox signals, and highlight the potential of alternative electron shuttles to investigate the link(s) between photosynthesis and plant development. [ABSTRACT FROM AUTHOR]

Plants, especially on the leaves, have various bioactive compounds capable of becoming natural growth promoters. Plant leaf extracts have been widely studied for their ability as an antibiotic substitute for broiler chickens. This meta-analysis study was aimed to assess the effectiveness of supplementations with leaf extract on the growth performance of broiler chickens, using average daily feed intake (ADFI), average daily gain (ADG), final body weight (FBW), and feed conversion ratio (FCR) as responses observed criteria. The meta-analysis study was based on the articles published from 2006 to recent years as several countries started to ban in-feed antibiotics. Databases (PubMed, Scopus, Directory of Open Access Journals [DOAJ], and ScienceDirect) were searched for peer-reviewed randomised controlled trials (RCTs) published in English. The meta-analysis included 19 research papers that met the criteria. Overall results showed a significant increase (P < 0.001) in ADFI by 0.56 g/day (95% confidence interval [CI] = 0.02 to 1.11), in ADG by 1.57 g/day (95% CI = 0.77 to 2.36), and in FBW by 2.28 (95% CI = 1.40 to 3.16). At the same time, the FCR was reduced (P < 0.001) by -1.25 (95% CI = -1.76 to -0.73) relative to controls taking cognisance of publication bias and heterogeneity. Results in the current meta-analysis study indicated that herbal feed additives were proven to be effective as growth promoters in broiler chickens. [ABSTRACT FROM AUTHOR]

The leaf is a determinate organ with a final size under genetic control. Numerous factors that regulate the final leaf size have been identified in Arabidopsis thaliana ; although most of these factors play their roles during the growth of leaf primordia, much less is known about leaf initiation and its effects on the final leaf size. In this study, we characterized oligocellula6-D (oli6-D), a semidominant mutant of A. thaliana with smaller leaves than the wild type (WT) due to its reduced leaf cell numbers. A time-course analysis showed that oli6-D had approximately 50% fewer leaf cells even immediately after leaf initiation; this difference was maintained throughout leaf development. Next-generation sequencing showed that oli6-D had chromosomal duplications involving 2-kb and 3-Mb regions of chromosomes 2 and 4, respectively. Several duplicated genes examined had approximately 2-fold higher expression levels, and at least one gene acquired a new intron/exon structure due to a chromosome fusion event. oli6-D showed reduced auxin responses in leaf primordia, primary roots and embryos, as well as reduced apical dominance and partial auxin-resistant root growth. CRISPR-associated protein-9-mediated genome editing enabled the removal of a 3-Mb duplicated segment, the largest targeted deletion in plants thus far. As a result, oli6-D restored the WT leaf phenotypes, demonstrating that oli6-D is a gain-of-function mutant. Our results suggest a new regulatory point of leaf size determination that functions at a very early stage of leaf development and is negatively regulated by one or more genes located in the duplicated chromosomal segments. [ABSTRACT FROM AUTHOR]

A two-year field experiment was conducted in two consecutive seasons to evaluate the response of wheat to pre-emergence (PRE) and early post-emergence (EPOST) herbicides. The herbicides prosulfocarb (3200 g ai ha−1) and chlorotoluron plus diflufenican (1380 + 92 g ai ha−1) were applied PRE, whereas the herbicides flufenacet plus diflufenican (240 + 120 g ai ha−1) and flufenacet plus diflufenican plus metribuzin (119.7 + 119.7 + 44.8 g ai ha−1) were applied EPOST at the second leaf growth stage of wheat. Heavy rainfalls that followed the PRE treatments and cold temperatures that occurred during the EPOST applications resulted in crop injuries and reduced yields in prosulfocarb, chlorotoluron plus diflufenican and flufenacet plus diflufenican the first and the second year, respectively. Weather conditions such as heavy rainfalls and low temperatures that may occur during and after herbicide treatments should be considered to avoid crop injuries and increase crop safety. [ABSTRACT FROM AUTHOR]

The polar auxin transport is required for proper plant growth and development. D6 PROTEIN KINASE (D6PK) is required for the phosphorylation of PIN‐FORMED (PIN) auxin efflux carriers to regulate auxin transport, while the regulation of D6PK stabilization is still poorly understood. Here, we found that Cytosolic ABA Receptor Kinases (CARKs) redundantly interact with D6PK, and the interactions are dependent on CARKs' kinase activities. Similarly, CARK3 also could interact with paralogs of D6PK, including D6PKL1, D6PKL2, and D6PKL3. The genetic analysis shows that D6PK acts the downstream of CARKs to regulate Arabidopsis growth, including hypocotyl, leaf area, vein formation, and the length of silique. Loss‐of‐function of CARK3 in overexpressing GFP‐D6PK plants leads to reduce the level of D6PK protein, thereby rescues plant growth. In addition, the cell‐free degradation assays indicate that D6PK is degraded through 26 S proteasome pathway, while the phosphorylation by CARK3 represses this process in cells. In summary, D6PK stabilization by the CARK family is required for auxin‐mediated plant growth and development. Summary statement: The AGC VIII kinase D6 PROTEIN KINASE (D6PK) phosphorylates PIN‐FORMED (PIN) auxin efflux carriers, which is essential for auxin transport in plant cells. This study uncovers a new mechanism for D6PK stability through Cytosolic ABA Receptor Kinases‐mediated phosphorylation to regulate auxin‐controlled Arabidopsis growth and development. [ABSTRACT FROM AUTHOR]

Auxin Response Factors (ARFs) make up a plant-specific transcription factor family that mainly couples perception of the phytohormone, auxin, and gene expression programs and plays an important and multi-faceted role during plant growth and development. Lemongrass (Cymbopogon flexuosus) is a representative Cymbopogon species widely used in gardening, beverages, fragrances, traditional medicine, and heavy metal phytoremediation. Biomass yield is an important trait for several agro-economic purposes of lemongrass, such as landscaping, essential oil production, and phytoremediation. Therefore, we performed gene mining of CfARFs and identified 26 and 27 CfARF-encoding genes in each of the haplotype genomes of lemongrass, respectively. Phylogenetic and domain architecture analyses showed that CfARFs can be divided into four groups, among which groups 1, 2, and 3 correspond to activator, repressor, and ETTN-like ARFs, respectively. To identify the CfARFs that may play major roles during the growth of lemongrass plants, RNA-seq was performed on three tissues (leaf, stem, and root) and four developmental stages (3-leaf, 4-leaf, 5-leaf. and mature stages). The expression profiling of CfARFs identified several highly expressed activator and repressor CfARFs and three CfARFs (CfARF3, 18, and 35) with gradually increased levels during leaf growth. Haplotype-resolved transcriptome analysis revealed that biallelic expression dominance is frequent among CfARFs and contributes to their gene expression patterns. In addition, co-expression network analysis identified the modules enriched with CfARFs. By establishing orthologous relationships among CfARFs, sorghum ARFs, and maize ARFs, we showed that CfARFs were mainly expanded by whole-genome duplications, and that the duplicated CfARFs might have been divergent due to differential expression and variations in domains and motifs. Our work provides a detailed catalog of CfARFs in lemongrass, representing a first step toward characterizing CfARF functions, and may be useful in molecular breeding to enhance lemongrass plant growth. [ABSTRACT FROM AUTHOR]

Although seagrasses are expected to thrive in future acidified oceans by overcoming low CO2 diffusion into plant tissues, the co‐occurrence of environmental stressors may affect their growth. Volcanic CO2 vents are often associated with toxic gases and metal‐rich fluids representing ideal sites to assess the effects of multiple stressors. We evaluated the response of Posidonia oceanica growing near shallow CO2 vents characterized by H2S spill‐out by comparing meadow structure and phenology to an area with no gas emissions. Seagrass descriptors at meadow, shoot and leaf level indicated that P. oceanica experienced stressful conditions at the vent area, in clear contrast to the flourishing features of P. oceanica previously described at CO2 vents with no evidence of toxic inputs. Furthermore, the reduction in both leaf δ34S and growth at the vent area indicates that sulfide intrusion occurs and affects seagrass growth performance, dampening the expected beneficial effects of high CO2 levels. [ABSTRACT FROM AUTHOR]

Phosphorus (P) deficiency is one of the main reasons limiting plant production of Brassica napus L. Exploring the dynamics of leaf intracellular substances and the correlations with photosynthesis and growth helps to understand the response mechanisms of B. napus L. to P deficiency. This study conducted experiments on B. napus L. plants by measuring the leaf electrophysiological parameters, leaf structure, elastic modulus (Em), photosynthesis, and growth indices under different P treatment conditions. The dynamics of leaf intracellular water and nutrients of B. napus L. were calculated and analyzed by using the electrophysiological parameters, and the plant tolerance threshold to low-P stress was discovered. The results indicated that the status of the leaf intracellular water and nutrients remained stable when the P concentration was not lower than 0.250 mmol·L−1, but maximized the photosynthesis and growth at a P level of 0.250 mmol·L−1. The 0.125 mmol·L−1 P concentration significantly decreased the mesophyll cell volume, and the palisade–sponge ratio and tightness degree of leaf tissue structure were remarkably increased. This led to an increase in cell elastic modulus, and significantly improved the water retention capacity of leaf cells. At the same time, the intracellular water use efficiency and total nutrient transport capacity of leaves remained stable. As a result, the photosynthesis and growth of plants were maintained at the same level as that of the control group. However, photosynthesis and growth were clearly inhibited with a further decrease in P concentration. Therefore, 0.125 mmol·L−1 was the tolerance threshold of B. napus L. to low P. With the help of electrophysiological information, the effects of the dynamics of intracellular substances on photosynthesis and growth of B. napus L. under low-P stress can be investigated, and the plant's adaptive response can be revealed. However, the findings of the current hydroponic study are not directly applicable to field conditions with naturally P-deficient soils. [ABSTRACT FROM AUTHOR]

This study examines the growth patterns, leaf morpho-anatomical traits and physiological characteristics of three ornamental taxa of Alocasia: A. melo, A reginae and Alocasia × morfontanensis 'Bambino'. Using optical and scanning electron microscopy, the research obtained anatomical details of the leaves. Alocasia × morfontanensis 'Bambino' exhibited the highest leaf growth rate among the taxa studied, showing significant leaf number increases from the 8th to the 12th week after planting. Alocasia melo produced 2–3 new basal shoots after 20 weeks, whereas the other two taxa did not produce any. Alocasia melo displayed the greatest relative water content and leaf thickness, while A. reginae showed the highest chlorophyll stability. This study also revealed variations in the adaxial and abaxial leaf color, stomatal width, epidermal thickness, and elemental composition among the taxa involved, with A. melo containing rhodium and high levels of calcium on their leaf surface. These findings contribute to the understanding of the Alocasia leaf morpho-anatomy and growth, which is useful for optimizing Alocasia cultivation and production across diverse ecosystems. [ABSTRACT FROM AUTHOR]

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Although sorghum (Sorghum bicolor L.), a C4 grass plant, requires a higher iron (Fe) concentration in its leaves for sufficient growth compared to C3 grass plants, there are differences in Fe-deficiency tolerance among sorghum genotypes. C4 plant sorghum localizes Fe in bundle sheath (BS) cells as a major site for CO2 assimilation; however, the response of chloroplasts in BS cells to Fe deficiency remains unknown. We compared the allocation of Fe and proteins involved in the photosystems between Fe-deficiency-tolerant and Fe-deficiency-susceptible sorghum genotypes. The distribution patterns of the Fe-requiring core proteins of photosystems I and II were the same in both genotypes, whereas the tolerant genotype, line D100, allocated more Fe to the BS thylakoid membranes than the susceptible genotype, regardless of Fe nutritional status and leaf Fe content. These results suggest that the tolerant D100 sorghum line has mechanisms to supply sufficient Fe to the BS thylakoid membranes. [ABSTRACT FROM AUTHOR]

Sodium chloride (NaCl) can cause oxidative stress in plants, which represents a potential obstacle to the development of monocultures worldwide. Onion (Allium cepa L.) is a famous vegetable consumed and used in world cuisine. In the present study, we analyzed the influence of soil physicochemical profile and the remedial capacity of linalool on seed emergence, roots, and leaf growth in onions subjected to salt stress, as well as its in vivo and in vitro antioxidant potential, Fe2+chelating activity, and reducing power of Fe3+. The outcome of the soil analysis established the following order of abundance: sulfur (S) > calcium (Ca) > potassium (K) > magnesium (Mg) > sodium (Na). NaCl (150 mM) significantly reduced the emergence speed index (ESI), leaf and root length, while increasing the peroxidation content. The length of leaves and roots significantly increased after treatment with linalool (300 and 500 μg/mL). Our data showed negative correlations between seed emergence and K+ concentration, which was reversed after treatments. Linalool (500 μg/mL) significantly reduced oxidative stress, but increased Fe2+ concentration and did not show potential to reduce Fe3+. The in vivo antioxidant effect of linalool is thought to primarily result from an enzymatic activation process. This mechanism underscores its potential as a therapeutic agent for oxidative stress–related conditions. Further investigation into this process could unveil new avenues for antioxidant therapy. [ABSTRACT FROM AUTHOR]

[Objective] Using Chrysanthemum morifolium HETAI, a characteristic medicinal and edible plant in Zhaoqing, Guangdong, as the material, to explore the growth characteristics and nutritional changes of leaves and inflorescences. [Method] The growth of leaves and floral organs from Chrysanthemum morifolium was measured in the field, and corresponding colorimetric methods were used to explore the content changes of effective components. [Result] The Chrysanthemum morifolium HETAI developed from young leaves to mature leaves for about 168 days, with significant morphological changes during the leaf development process. The mature leaves were oval shaped, feathered with shallow or deep cracks, and the leaf edges were round serrated with notches. The growth cycle of flower organs was about 55 days, and it lasted for about 20 days from the initial flowering period to the full-bloom period. The tongue shaped and tubular flower clusters were fully developed; the flower diameter could reach 4.67-4.89 cm. During the development of flower organs, the content of soluble proteins generally showed a downward trend, but the content of total flavonoids and soluble sugars continued to increase, both contents maintain high levels during the development stage of 3140 days. Combined with the growth characteristics of leaves and inflorescences, the initial flowering period and early peak flowering period (31-40 days) were the suitable harvest time for this variety of chrysanthemum. [Conclusion] This study provides a basis for the cultivation standards of advantageous varieties in the characteristic agriculture (Nanyao) science and technology park of Gaoyao District, Guangdong Province [ABSTRACT FROM AUTHOR]

Summary: Non‐structural carbohydrates (NSCs), as the labile fraction and dominant carbon currency, are essential mediators of plant adaptation to environments. However, whether and how NSC coordinates with plant economic strategy frameworks, particularly the well‐recognized leaf economics spectrums (LES) and root economics space (RES), remains unclear.We examined the relationships between NSC and key plant economics traits in leaves and fine roots across 90 alpine coniferous populations on the Tibetan Plateau, China.We observed contrasting coordination of NSC with economics traits in leaves and roots. Leaf total NSC and soluble sugar aligned with the leaf economic spectrum, conveying a trade‐off between growth and storage in leaves. However, NSC in roots was independent of the root economic spectrum, but highly coordinated with root foraging, with more starch and less sugar in forage‐efficient, thinner roots. Further, NSC‐trait coordination in leaves and roots was, respectively, driven by local temperature and precipitation.These findings highlight distinct roles of NSC in shaping the above‐ and belowground multidimensional economics trait space, and NSC‐based carbon economics provides a mechanistic understanding of how plants adapt to heterogeneous habitats and respond to environmental changes. [ABSTRACT FROM AUTHOR]

Background and Aims Intra- and transgenerational plasticity may provide substantial phenotypic variation to cope with environmental change. Since assessing the unique contribution of the maternal environment to the offspring phenotype is challenging in perennial, outcrossing plants, little is known about the evolutionary and ecological implications of transgenerational plasticity and its persistence over the life cycle in these species. We evaluated how intra- and transgenerational plasticity interplay to shape the adaptive responses to drought in two perennial Mediterranean shrubs. Methods We used a novel common garden approach that reduced within-family genetic variation in both the maternal and offspring generations by growing the same maternal individual in two contrasting watering environments, well-watered and drought, in consecutive years. We then assessed phenotypic differences at the reproductive stage between offspring reciprocally grown in the same environments. Key Results Maternal drought had an effect on offspring performance only in Helianthemum squamatum. Offspring of drought-stressed plants showed more inflorescences, less sclerophyllous leaves and higher growth rates in both watering conditions, and heavier seeds under drought, than offspring of well-watered maternal plants. Maternal drought also induced similar plasticity patterns across maternal families, showing a general increase in seed mass in response to offspring drought, a pattern not observed in the offspring of well-watered plants. In contrast, both species expressed immediate adaptive plasticity, and the magnitude of intragenerational plasticity was larger than the transgenerational plastic responses. Conclusions Our results highlight that adaptive effects associated with maternal drought can persist beyond the seedling stage and provide evidence of species-level variation in the expression of transgenerational plasticity. Such differences between co-occurring Mediterranean species in the prevalence of this form of non-genetic inheritance may result in differential vulnerability to climate change. [ABSTRACT FROM AUTHOR]

The aim of this work was to investigate the effect of synthesized magnesium oxide nanoparticles of Jatropha tajonensis leaf extract on the growth and yield of cowpea (Vigna unguiculata (L.) Walp.). Materials and Methods. The preparation and planting of the cowpea seeds; The extraction of extract of Jatropha tajonensis leaves in aqueous solution. The synthesis of MgO nanoparticles from the extract, followed by characterization to confirm the formation - UV-VIS, FTIR, SEM-EDX and PXRD. The effects of MgONPs on cowpea (Vigna unguiculata (L.) Walp.) plants were surveyed under field conditions to assess its uses in improving growth and yield of cowpea. Results. The results showed that different doses of MgONPs applied to cowpea plant significantly affected all measured parameters of cowpea plantlets under the field condition in a positive way. The best results in growth, yield and the phonological parameters were cowpea plants treated with high MgONP applications (100 mg/L). It has been observed that different MgONPs applications have significant effects on vegetative growth and yield parameters of cowpea. A significant increase in the number of vegetative parameters was observed in the pots with different doses of nano-20, 40, 60, 80 and 100/MgONPs applications compared to the control. Different MgO (with or without NPs) treatments led to significant differences in shoot formation (P < 0.01). According to the effect of different doses of magnesium NPs applied to the cowpea, plant height varied between 18.88 ± 2.51 and 21.35 ± 3.25. The highest value in the height was obtained from nano-100 mg/L MgONPs application with 21.35 ± 3.25 and the lowest value was obtained from the salt 17.48 ± 3.83 mg/L MgONPs application. Conclusion. This study found that MgONPs greatly influenced the plantlets' growth parameters and other measured traits; in addition. There was an indication that the efficiency of growth and yield of cowpea could be improved by increased application of MgO in the form of nanoparticles. Also, highlighted was the possibility of using MgONPs in increasing another crop yield to cater for the evergrowing world population. [ABSTRACT FROM AUTHOR]

A feeding trial was conducted to discern the influence of dietary incorporation of Moringa oleifera leaf meal on growth performance and nutrient utilization of Japanese quail for 6 weeks. A total 288 day-old Japanese quail chicks were randomly distributed into eight treatments of 36 Japanese quail chicks per treatment with three replicates of 12 quail in each. Japanese quail of treatment T1 (control group) were fed a basal diet (starter and finisher), whereas in treatment group T2 basal diet was incorporated with Vitamin C @200 mg/kg, T3 basal diet was incorporated with Vitamin E @10 IU/kg, and in diets T4, T5, T6, T7, and T8, the basal diet was incorporated with 1.0%, 3.0%, 5.0%, 7.0% and 9.0% Moringa oleifera leaf meal, respectively. Results showed that incorporation of 3.0% Moringa oleifera leaf meal improved the performance of Japanese quail in terms of body weight gain and performance index during the overall period. The maximum crude protein utilization was seen in 3.0% Moringa oleifera leaf meal incorporated group of Japanese quail followed by 1.0%. However, maximum ether extract utilization was seen with 1.0% Moringa oleifera leaf meal while ether extract and crude protein utilization was lower in 9.0% Moringa oleifera leaf meal incorporated group. Average feed cost per kg weight gain differed in Japanese quail offered various dietary treatment. It can be concluded that 3.0 % Moringa oleifera leaf meal can be incorporated in feed for improvement in growth performance of Japanese quail. [ABSTRACT FROM AUTHOR]

Exploring the growth and physiological response mechanisms of rice under continuous drought stress circumstances can provide a significant scientific foundation and technological assistance for meeting drought difficulties, improving drought resistance and rice (Oryza sativa L.) output, and ensuring food security. In this study, a rice field experiment was conducted under a rain shelter with five different treatments set up: P1 (drought stress from tillering stage), P2 (drought stress from jointing–booting stage), P3 (drought stress from heading–flowering stage), P4 (drought stress from grain filling stage), and CK (adequate water management throughout the growth stage). Continuous drought stress from different growth stages with four levels (mild, medium, moderate, and severe). The results showed that the effects of different drought stress treatments on rice growth varied significantly. Compared with the CK treatment, plant height was reduced by 12.10%, 8.14%, 3.83%, and 1.06% in the P1, P2, P3, and P4 treatments, respectively, and the number of tillers was reduced by 23.83%, 18.91%, 13.47%, and 8.68%, respectively. With the increase in drought stress levels, SPAD values and Rubisco activity of rice leaf continued to decrease; SOD activity showed a decreasing trend, but the decreasing trend of POD and CAT activities was not significant, while MDA content showed an increasing trend. For yield components, continuous drought stress significantly reduced spike length of rice by an average of 3.5%, effective number of spikes by 18.9%, thousand grain weight by 3.7%, grain number per spike by 11.6%, and fruiting rate by 1.8%, respectively, compared to CK treatments during the growth period. In general, continuous drought stress during the early growth period affected the effective spike number and the grain number per spike. Continuous drought stress after the grain filling stage had the least effect on yield (17.62% of yield reduction), and water use efficiency (1.76 kg m−3) was much higher than other treatments. These researchers' findings provide insight into how rice physiology and growth react to continuous drought stress, which is significant for agricultural operations. [ABSTRACT FROM AUTHOR]