10 results on '"LeBlanc, AM"'
Search Results
2. world
- Author
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Monsinjon, JR, Wyneken, J, Rusenko, K, Lopez-Mendilaharsu, M, Lara, P, Santos, A, dei Marcovaldi, MAG, Fuentes, MMPB, Kaska, Y, Tucek, J, Nel, R, William, KL, LeBlanc, AM, Rostal, D, Guillon, JM, and Girondot, M more...
- Subjects
Climate change ,Phenotypic plasticity ,Phenology ,Hatching success ,Sex ,Caretta caretta ,ratio ,Embryonic development ,Incubation ,Thermal tolerance ,Reptile - Abstract
Phenological shifts, by initiating reproductive events earlier, in response to advanced seasonal warming is one of the most striking effects currently observed in wild populations. For sea turtles, phenological adjustment to warming conditions could be the most effective short-term adaptation option against climate change. We calculated future phenological changes required in seven important loggerhead (Caretta caretta) nesting populations to continue achieving a high hatching success and a sex ratio that lies within current ranges. Considering temperature-mediated phenological changes, we found that most populations (six out of seven) will not be able to keep pace with a warming climate. Under an optimistic climate warming scenario (RCP4.5), these populations will face a climatic debt, that is, a difference between required and expected phenological changes, and warming will substantially reduce hatching success and induce a feminization of hatchlings, which may jeopardize their reproductive sustainability. Our approach offers the possibility to quantify the efficiency of phenological shifts in oviparous reptiles by considering physiological, developmental and phenological processes. C1 [Monsinjon, Jonathan R.; Guillon, Jean-Michel; Girondot, Marc] Univ Paris Saclay, Univ Paris Sud, Lab Ecol Systemat Evolut, CNRS,AgroParisTech, F-91405 Orsay, France. [Wyneken, Jeanette] Florida Atlantic Univ, Dept Biol Sci, Boca Raton, FL 33431 USA. [Rusenko, Kirt] Gumbo Limbo Nat Ctr, 1801 N Ocean Blvd, Boca Raton, FL 33432 USA. [Lopez-Mendilaharsu, Milagros; Lara, Paulo; Santos, Alexsandro; dei Marcovaldi, Maria A. G.] Fundacao Pro Tamar, Rua Rubens Guelli,134 Sala 307, Salvador, BA, Brazil. [Fuentes, Mariana M. P. B.] Florida State Univ, Dept Earth Ocean & Atmospher Sci, Marine Turtle Res Ecol & Conservat Grp, North Woodward Ave, Tallahassee, FL 32306 USA. [Kaska, Yakup] Pamukkale Univ, Sea Turtle Res Ctr DEKAMER, Denizli, Turkey. [Tucek, Jenny; Nel, Ronel] Nelson Mandela Univ, Dept Zool, ZA-6031 Port Elizabeth, South Africa. [William, Kristina L.] Caretta Res Project, POB 9841, Savannah, GA 31412 USA. [LeBlanc, Anne-Marie; Rostal, David] Georgia Southern Univ, Dept Biol, Statesboro, GA 30460 USA. more...
- Published
- 2019
Catalog
3. Temperature-dependent sex determination in the Kemp’s ridley sea turtle: effects of incubation temperatures on sex ratios
- Author
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LeBlanc, AM, primary, Wibbels, T, additional, Shaver, D, additional, and Walker, JS, additional
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- 2012
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4. Unlocking
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LEBLANC, AMY and LEBLANC, AMY
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- 2021
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5. Adaptation of sea turtles to climate warming: Will phenological responses be sufficient to counteract changes in reproductive output?
- Author
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Fuentes MMPB, Santos AJB, Abreu-Grobois A, Briseño-Dueñas R, Al-Khayat J, Hamza S, Saliba S, Anderson D, Rusenko KW, Mitchell NJ, Gammon M, Bentley BP, Beton D, Booth DTB, Broderick AC, Colman LP, Snape RTE, Calderon-Campuzano MF, Cuevas E, Lopez-Castro MC, Flores-Aguirre CD, Mendez de la Cruz F, Segura-Garcia Y, Ruiz-Garcia A, Fossette S, Gatto CR, Reina RD, Girondot M, Godfrey M, Guzman-Hernandez V, Hart CE, Kaska Y, Lara PH, Marcovaldi MAGD, LeBlanc AM, Rostal D, Liles MJ, Wyneken J, Lolavar A, Williamson SA, Manoharakrishnan M, Pusapati C, Chatting M, Mohd Salleh S, Patricio AR, Regalla A, Restrepo J, Garcia R, Santidrián Tomillo P, Sezgin C, Shanker K, Tapilatu F, Turkozan O, Valverde RA, Williams K, Yilmaz C, Tolen N, Nel R, Tucek J, Legouvello D, Rivas ML, Gaspar C, Touron M, Genet Q, Salmon M, Araujo MR, Freire JB, Castheloge VD, Jesus PR, Ferreira PD, Paladino FV, Montero-Flores D, Sozbilen D, and Monsinjon JR more...
- Subjects
- Animals, Temperature, Climate Change, Reproduction, Sex Ratio, Turtles physiology
- Abstract
Sea turtles are vulnerable to climate change since their reproductive output is influenced by incubating temperatures, with warmer temperatures causing lower hatching success and increased feminization of embryos. Their ability to cope with projected increases in ambient temperatures will depend on their capacity to adapt to shifts in climatic regimes. Here, we assessed the extent to which phenological shifts could mitigate impacts from increases in ambient temperatures (from 1.5 to 3°C in air temperatures and from 1.4 to 2.3°C in sea surface temperatures by 2100 at our sites) on four species of sea turtles, under a "middle of the road" scenario (SSP2-4.5). Sand temperatures at sea turtle nesting sites are projected to increase from 0.58 to 4.17°C by 2100 and expected shifts in nesting of 26-43 days earlier will not be sufficient to maintain current incubation temperatures at 7 (29%) of our sites, hatching success rates at 10 (42%) of our sites, with current trends in hatchling sex ratio being able to be maintained at half of the sites. We also calculated the phenological shifts that would be required (both backward for an earlier shift in nesting and forward for a later shift) to keep up with present-day incubation temperatures, hatching success rates, and sex ratios. The required shifts backward in nesting for incubation temperatures ranged from -20 to -191 days, whereas the required shifts forward ranged from +54 to +180 days. However, for half of the sites, no matter the shift the median incubation temperature will always be warmer than the 75th percentile of current ranges. Given that phenological shifts will not be able to ameliorate predicted changes in temperature, hatching success and sex ratio at most sites, turtles may need to use other adaptive responses and/or there is the need to enhance sea turtle resilience to climate warming., (© 2023 The Authors. Global Change Biology published by John Wiley & Sons Ltd.) more...
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- 2024
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6. Systematic Optimization of the iMALDI Workflow for the Robust and Straightforward Quantification of Signaling Proteins in Cancer Cells.
- Author
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Froehlich BC, Popp R, Sobsey CA, Ibrahim S, LeBlanc AM, Mohammed Y, Aguilar-Mahecha A, Poetz O, Chen MX, Spatz A, Basik M, Batist G, Zahedi RP, and Borchers CH
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- Cell Line, Tumor, Humans, Limit of Detection, Time Factors, Neoplasm Proteins metabolism, Signal Transduction, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Workflow
- Abstract
Purpose: Immuno-MALDI (iMALDI) combines immuno-enrichment of biomarkers with MALDI-MS for fast, precise, and specific quantitation, making it a valuable tool for developing clinical assays. iMALDI assays are optimized for the PI3-kinase signaling pathway members phosphatase and tensin homolog (PTEN) and PI3-kinase catalytic subunit alpha (p110α), with regard to sensitivity, robustness, and throughput. A standardized template for developing future iMALDI assays, including automation protocols to streamline assay development and translation, is provided., Experimental Design: Conditions for tryptic digestion and immuno-enrichment (beads, bead:antibody ratios, incubation times, direct vs. indirect immuno-enrichment) are rigorously tested. Different strategies for calibration and data readout are compared., Results: Digestion using 1:2 protein:trypsin (wt:wt) for 1 h yielded high and consistent peptide recoveries. Direct immuno-enrichment (antibody-bead coupling prior to antigen-enrichment) yielded 30% higher peptide recovery with a 1 h shorter incubation time than indirect enrichment. Immuno-enrichment incubation overnight yielded 1.5-fold higher sensitivities than 1 h incubation. Quantitation of the endogenous target proteins is not affected by the complexity of the calibration matrix, further simplifying the workflow., Conclusions and Clinical Relevance: This optimized and automated workflow will facilitate the clinical translation of high-throughput sensitive iMALDI assays for quantifying cell-signaling proteins in individual tumor samples, thereby improving patient stratification for targeted treatment., (© 2020 The Authors. Proteomics - Clinical Applications published by Wiley-VCH GmbH.) more...
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- 2020
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7. Multiplexed MRM-based assays for the quantitation of proteins in mouse plasma and heart tissue.
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Percy AJ, Michaud SA, Jardim A, Sinclair NJ, Zhang S, Mohammed Y, Palmer AL, Hardie DB, Yang J, LeBlanc AM, and Borchers CH
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- Animals, Biomarkers blood, Chromatography, Liquid methods, Isotope Labeling, Mass Spectrometry methods, Mice, Mice, Inbred C57BL, Myocardium chemistry, Blood Proteins analysis, Myocardium metabolism
- Abstract
The mouse is the most commonly used laboratory animal, with more than 14 million mice being used for research each year in North America alone. The number and diversity of mouse models is increasing rapidly through genetic engineering strategies, but detailed characterization of these models is still challenging because most phenotypic information is derived from time-consuming histological and biochemical analyses. To expand the biochemists' toolkit, we generated a set of targeted proteomic assays for mouse plasma and heart tissue, utilizing bottom-up LC/MRM-MS with isotope-labeled peptides as internal standards. Protein quantitation was performed using reverse standard curves, with LC-MS platform and curve performance evaluated by quality control standards. The assays comprising the final panel (101 peptides for 81 proteins in plasma; 227 peptides for 159 proteins in heart tissue) have been rigorously developed under a fit-for-purpose approach and utilize stable-isotope labeled peptides for every analyte to provide high-quality, precise relative quantitation. In addition, the peptides have been tested to be interference-free and the assay is highly multiplexed, with reproducibly determined protein concentrations spanning >4 orders of magnitude. The developed assays have been used in a small pilot study to demonstrate their application to molecular phenotyping or biomarker discovery/verification studies., (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) more...
- Published
- 2017
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8. Butyrylcholinesterase activity in multiple sclerosis neuropathology.
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Darvesh S, Leblanc AM, Macdonald IR, Reid GA, Bhan V, Macaulay RJ, and Fisk JD
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- Acetylcholinesterase metabolism, Adult, Aged, Aged, 80 and over, Brain metabolism, Case-Control Studies, Female, Humans, Immunohistochemistry, Inflammation enzymology, Inflammation metabolism, Inflammation pathology, Male, Microglia enzymology, Microglia pathology, Middle Aged, Multiple Sclerosis metabolism, Myelin Sheath enzymology, Myelin Sheath pathology, Brain enzymology, Brain pathology, Butyrylcholinesterase metabolism, Multiple Sclerosis enzymology, Multiple Sclerosis pathology
- Abstract
Butyrylcholinesterase (BuChE) is an enzyme capable of hydrolysing a wide variety of esters including acetylcholine, a molecule involved in neurotransmission and modulation of immune cell activity. In the brain, BuChE is expressed in white matter and certain populations of neurons and glia. Multiple sclerosis (MS) is an autoimmune disease affecting white matter characterized by neuroinflammation and neurodegeneration in the central nervous system. Here we demonstrate alterations in BuChE activity in MS white matter lesions, including diminished enzyme activity associated with myelin and an increased activity in cells with microglial morphology. Increased BuChE activity within MS lesions could contribute to the pro-inflammatory immune responses through hydrolysis of acetylcholine and to demyelination through hydrolytic deacylation of myelin proteins such as proteolipid protein. This suggests that BuChE could be a potential target for novel disease-modifying strategies for MS., (Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved.) more...
- Published
- 2010
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9. Effect of daily water treatment on hatchling sex ratios in a turtle with temperature-dependent sex determination.
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Leblanc AM and Wibbels T
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- Animals, Sex Differentiation drug effects, Sex Ratio, Temperature, Turtles physiology, Water pharmacology
- Abstract
Some previous studies indicate that the local hydric environment may influence sex determination in turtles with temperature-dependent sex determination. In this study, the effect of a daily application of 0.77 mL of ddH(2)0 per egg using an incubation temperature of 29.1 degrees C was examined during the temperature-sensitive period for two consecutive nesting seasons. This regimen yielded sex ratios of 11.8 and 11.1% male in control groups not receiving water supplementation, whereas daily water treatments resulted in sex ratios of 86.7 and 45.7% male during the 2006 and 2007 nesting seasons, respectively. The results indicate that daily water treatments significantly influenced sex ratios (P<0.001). In addition to providing insight on the physiology of sex determination, these results could have implications for studies predicting sex ratios from nests on natural nesting beaches that are periodically exposed to rain., (2008 Wiley-Liss, Inc) more...
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- 2009
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10. Conjugated linoleic acids, atherosclerosis, and hepatic very-low-density lipoprotein metabolism.
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McLeod RS, LeBlanc AM, Langille MA, Mitchell PL, and Currie DL
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- Animals, Dietary Supplements, Humans, Liver drug effects, Receptors, Cytoplasmic and Nuclear, Transcription Factors, Arteriosclerosis prevention & control, Linoleic Acids, Conjugated administration & dosage, Linoleic Acids, Conjugated pharmacology, Lipoproteins, VLDL metabolism, Liver metabolism
- Abstract
Conjugated linoleic acids (CLAs) are isomeric forms of the 18:2 fatty acid that contain conjugated sites of unsaturation. Although CLAs are minor components of the diet, they have many reported biological activities. For nearly a decade, the potential for CLA to modify the atherosclerotic process has been examined in animal models, and studies of supplementation of the human diet with CLA were started with the anticipation that such an intervention could also reduce the risk of cardiovascular disease. Central to the hypothesis is the expectation that dietary modification could alter plasma lipid and lipoprotein metabolism toward a more cardioprotective profile. This review examines the evidence in support of the hypothesis and the mechanistic studies that lend support for a role of CLA in hepatic lipid and lipoprotein metabolism. Although there are still limited studies in strong support of a role for CLA in the reduction of early atherosclerotic lesions, there has been considerable progress in defining the mechanisms of CLA action. CLA could primarily modulate the metabolism of fatty acids in the liver. The tools are now available to examine isomer-specific effects of CLA on hepatic lipid and lipoprotein metabolism and the potential of CLA to modify hepatic gene expression patterns. Additional animal and cell culture studies will increase our understanding of these unusual fatty acids and their potential for health benefits in humans. more...
- Published
- 2004
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