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Psychological thriving is crucial for the sustainable well-being and continuous growth of employees in the workplace. This study aims to explore the concept, measurement, and heterogeneity of dualistic psychological thriving at work to promote sustainable vitality and learning among employees. In Study 1, we identified psychological thriving at work as a second-order dualistic construct, encompassing the positive psychological state in which individuals experience vitality and learning in their current work and hold high expectations for their future work. This construct consists of two dimensions: psychological thriving of work experience and psychological thriving of work development. In Study 2, we conducted exploratory factor analysis and second-order confirmatory factor analyses using two independent samples, providing empirical evidence for the second-order dualistic structure of psychological thriving at work. Subsequently, we developed a comprehensive scale to measure dualistic psychological thriving at work and assessed its criterion validity by examining its relationship with workplace friendship and work autonomy. In Study 3, we investigated the overall levels and pursuit preferences of dualistic psychological thriving at work among different generations. The findings revealed significant differences in the overall levels and pursuit preferences of dualistic psychological thriving at work across generations. Post-1990s employees exhibited the highest overall level of dualistic psychological thriving at work, while post-1970s employees had the lowest. Moreover, post-1990s employees were more inclined to pursue psychological thriving of work experience, whereas post-1980s employees tended to seek psychological thriving of work development. No significant differences were found among post-1970s employees.

Urine is conventionally used as a specimen to document diazepam-related crimes; however, few reports have described the pharmacokinetics of diazepam and its metabolites in urine.This study aimed to investigate the pharmacokinetics of diazepam and its metabolites, including glucuronide compounds, in the urine of Chinese participants.A total of 28 volunteers were recruited and each participant ingested 5 mg of diazepam orally. Ten milliliters of urine were collected from each participant at post-consumption timepoints of prior (zero), 1, 2, 4, 8, 12, and 24 h and 2, 3, 6, 12, and 15 days. All samples were extracted by solid-phase extraction and analyzed using high-performance liquid chromatography-tandem mass spectrometry. Diazepam and its main metabolites, except for temazepam, were detected in the urine of volunteers. Pharmacokinetic parameters were analyzed using the pharmacokinetic software DAS according to the non-compartment model.Urinary diazepam peaked at 2.38 ng/mL (CThe results regarding diazepam pharmacokinetics in urine would be of great help in forensic science and drug screening.

The high surface energy of nanomaterials endows them a metastable nature, which greatly limits their application. However, in some cases, the degradation process derived from the poor stability of nanomaterials offers an unconventional approach to design and obtain functional nanomaterials. Herein, based on the poor stability of ZnSe-[DETA]0.5 hybrid nanobelts, we developed a new strategy to chemically graphitize and functionalize graphene oxide (GO). When ZnSe[DETA]0.5 hybrid nanobelts encountered a strong acid, they were attacked by H+ cations and could release highly reactive Se2− anions into the reaction solution. Like other common reductants (such as N2H4·H2O), these Se2− anions exhibited an excellent ability to restore the structure of GO. The structural restoration of GO was greatly affected by the reaction time, the volume of HCl, and the mass ratio between GO and ZnSe[DETA]0.5 nanobelts. By carefully controlling the reaction process and the post-processing process, we finally obtained several Se-based reduced GO (RGO) nanocomposites (such as ZnSe/Se-RGO, ZnSe-RGO, and Se-RGO) and various selenide/metal-RGO nanocomposites (such as Ag2Se-RGO, Cu2Se-RGO, and Pt-RGO). Although the original structure and composition of ZnSe[DETA]0.5 nanobelts are destroyed, the procedure presents an unconventional way to chemically graphitize and functionalize GO and thus provides a new material synthesis platform for nanocomposites.

The increases in frequency and intensity of drought worldwide has seriously affected tree growth, and even led to widespread forest mortality. Leaf traits estimated from pressure-volume (PV) curve provide key leaf physiological information that reflects the drought tolerance of trees. However, it is uncertain that which PV parameter performs the best at local scale. Here, we measured five PV traits (including TLP, π全球范围内干旱频率和强度的增加严重影响树木生长,甚至导致森林大面积死亡。压力-容积(PV)曲线能够反映树木对干旱的容忍能力,但在局域尺度上尚未确定哪个PV曲线参数具有最优指示性。通过测定东北温带森林20种主要树种(包括16种被子植物和4种裸子植物)的PV曲线性状,包括质壁分离时的相对含水量(RWC

Recent studies have shown that Transmembrane protein 100 (TMEM100) is a gene at locus 17q32 encoding a 134-amino acid protein with two hypothetical transmembrane domainsa, and first identified as a transcript from the mouse genome. As a downstream target gene of bone morphogenetic protein (BMP)-activin receptor-like kinase 1 (ALK1) signaling, it was activated to participate in inducing arterial endothelium differentiation, maintaining vascular integrity, promoting cell apoptosis, inhibiting metastasis and proliferation of cancer cells. However, evidence for the function of TMEM100 in inflammation is still limited. In this study, we explore the role of TMEM100 in inflammatory cytokine secretion and the role of MAPK signaling pathways in tumor necrosis factor-alpha (TNF-α)-induced TMEM100 expression in LX-2 cells. We found that the expression of TMEM100 was decreased markedly in human liver fibrosis tissues, and its expression was also inhibited in LX-2 cells induced by TNF-α, suggesting that it might be associated with the development of inflammation. Therefore, we demonstrated that overexpression of TMEM100 by transfecting pEGFP-C2-TMEM100 could lead to the down-regulation of IL-1β and IL-6 secretion. Moreover, we found that expression changes of TMEM100 could be involved in inhibition or activation of MAPK signaling pathways accompanied with regulating phosphorylation levels of ERK and JNK protein in response to TNF-α. These results suggested that TMEM100 might play an important role in the secretion of inflammatory cytokines (IL-1β and IL-6) of LX-2 cells induced by TNF-α, and MAPK (ERK and JNK) signaling pathways might participate in its induction of expression.

As of 14 October 2021, coronavirus disease 2019 (COVID-19) has affected more than 246 million individuals and caused more than 4.9 million deaths worldwide. COVID-19 has caused significant damage to the health, economy and lives of people worldwide. Although severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is not as lethal as SARS coronavirus or Middle East respiratory syndrome coronavirus, its high transmissibility has had disastrous consequences for public health and health-care systems worldwide given the lack of effective treatment at present.To clarify the mechanisms by which SARS-CoV-2 caused lung inflammation and injury, from the molecular mechanism to lung damage and tissue repair, from research to clinical practice, and then presented clinical requirements.References for this review were identified through searches '(COVID-19 [Title]) OR (SARS-CoV-2 [Title])' on PubMed, and focused on the pathological damage and clinical practice of COVID-19.We comprehensively reviewed the process of lung inflammation and injury during SARS-CoV-2 infection, including pyroptosis of alveolar epithelial cells, cytokine storm and thrombotic inflammatory mechanisms.This review describes SARS-CoV-2 in comparison to SARS and explores why most people have mild inflammatory responses, even asymptomatic infections, and only a few develop severe disease. It suggests that future therapeutic strategies may be targeted antiviral therapy, the pathogenic pathways in the lung inflammatory response, and enhancing repair and regeneration in lung injury.

An unprecedented base-promoted multi-component one-pot dearomatization of N-alkyl activated azaarenes was developed, which enabled the synthesis of complex and diverse bridged cyclic polycycles with multiple stereocenters in a highly regio- and diastereoselective manner. Besides, we realized the step-controlled dearomative bi- and trifunctionalization of quinolinium salts. These transformations not only achieved the maximization of the reaction sites of pyridinium, quinolinium and isoquinolinium salts to enhance structural complexity and diversity, but also opened up a new reaction mode of these N-activated azaarenes. A unique feature of this strategy is the use of easily accessible and bench-stable N-alkyl activated azaarenes to provide maximum reactive sites for dearomative cascade cyclizations. In addition, the salient characteristics including high synthetic efficiency, short reaction time, mild conditions and simple operation made this strategy particularly attractive., An unprecedented base-promoted multi-component one-pot dearomatization of N-alkyl activated azaarenes was developed to construct complex and diverse bridged cyclic polycycles with multiple stereocenters in a highly regio- and diastereoselective manner.

Objective: To evaluate the diagnostic efficiency of bronchoalveolar lavage fluid (BALF) for MycobacteriumTuberculosis (MTB) infection using laboratory methods. Methods: A retrospective study was conducted in patients diagnosed with active pulmonary tuberculosis (APTB) and lacking sputum quality/quantity. BALF collected during the operation processes of Electric bronchoscopy were tested using Ziehl-Neelsen staining acid-fast bacilli smear microscopy (Z-N-AFB-SM), GeneXpert MTB/RIF（Xpert），loop-mediated isothermal amplification (LAMP), or culturing with BACTEC™ Mycobacterial Growth Indicator Tube™ 960 (MGIT). Chi-square test was used for statistic analysis. Results: 331 suspected APTB patients were enrolled in this study. 224 of them were sputum-scarce. 89 were sputum-sufficient andnegative in both Z-N-AFB-SM and MGIT 960 testing. Of the sputum sufficient patients, BALF-testing confirmed APTB diagnosis in 20.2% (18/89) via Z-N-AFB-SM, and 53.0% (35/89) via MGIT. The total positive rates of BALF testing via four aforementioned methods were 18.2% (57/313), 66.4% (168/253), 61.0% (83/136) and 48.2% (140/290) respectively. The positive rate of MTB discovered in BALF collected by well-trained respiratory physicians are significantly higher than those collected by anesthetists (χ2=22.48, PConclusion: BALF has a similar sensitivity and specificity for APTB laboratory diagnosis. It can be used as a complementary diagnostic method for APTB when sputum availability is poor. The proficiency of BALF collection is an important factor affecting the detection results.

This investigation was conducted by means of personal contact. The interviewees were selected from universities and Internet cafes in Xi'an. In addition to the investigation of crowd characteristics of game players, there are two aspects involved in the investigation of preferences. One is the investigation of game style preferences. and the other is a survey of the habits and preferences of game players. The survey of game style preference includes the preference of drawing style, the preference of overall style, the preference basis of game characteristics and the influencing factors of game experience. According to the analysis of survey data, game players like Chinese style games with good visual perception, strong sense of substitution and easy to use in terms of style preference. In terms of the investigation on the habits of game players, according to the analysis of the investigation data, it is more likely to attract the players when the game starts to design charging items about half an hour; in addition to strengthening the competitiveness in terms of the game content and plot, it is also necessary to strengthen the competitive situation setting in the lower stage of the game, strengthen the sense of bringing in, and design equipment or other items with low price The key point of the design is to attract the players to go deep; the plot design of the game after half an hour and the longer time to attract the players are very important. In 2018, China's game market revenue reached 203.61 billion yuan, with mobile games accounting for 57%. The revenue of mobile games exceeded 100 billion yuan, while the revenue of end games continued to grow, with an increase of 11.4%. The revenue of page games is only 15.6 billion, showing negative growth for two consecutive years. The market revenue of e-games exceeds 70 billion, and the revenue of mobile e-games grows rapidly. IP game revenue reached 74.56 billion, accounting for 64.2% of mobile game revenue. The revenue of plane games reached 15.98 billion with an increase of 45%. In order to know which games will be more attractive to the game players and which embedded consumption items will be more attractive to the game players, a preference survey is carried out for the game players in order to understand the consumption characteristics of the main consumer groups of the game and provide reference for the majority of game companies to make games and design charging items.

In order to solve the shortcomings of the traditional chaotic encryption and problems of low security, the double chaotic image encryption algorithm based on fractional Fourier transform is proposed. In this algorithm, the optimization algorithm is obtained with the help of Henon mapping and fractional Fourier transforms, then the ciphertext image obtained by the optimization algorithm is taken as input, and according to the sequence of the optimal solution, the logistic chaos is synthesized to obtain the final ciphertext image. This algorithm combines chaotic systems and Fourier transforms, which allows the plaintext to be well hidden, and spatial and frequency domain scrambling is achieved. After experiments, the results show that the improved encryption algorithm achieves better encryption effect. It not only has strong sensitivity and large key space, but also can resist attacks effectively. It has certain application value in image information security.

This paper researches constructive generalized synchronization (GS) for bidirectional continuous array of differential systems (BCADS). The results give general representations of GS in BCADS. The effectiveness of the theoretical results has been shown according numerical simulations.

The mammalian target of rapamycin (mTOR) pathway is dysregulated in more than 50% of all human malignancies and is a major target in cancer treatment. In this study, we explored the underlying mechanism involving microRNA-145-3p (miR-145-3p) in the development and progression of non-small cell lung cancer (NSCLC) by targeting PDK1 via the mTOR signaling pathway. NSCLC tissues and adjacent normal tissues were obtained from 83 NSCLC patients. miR-145-3p, PDK1, and mTOR levels were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry. Human NSCLC cell lines A549 and H1299 were transfected with miR-145-3p and siPDK1 to confirm the effect of miR-145-3p and PDK1 on NSCLC cells in vitro. Cell growth was evaluated by a CCK8 assay. Cell motility and chemotaxis analysis were determined by the scratch test and chemotaxis assay, respectively. The protein levels of PDK1 and mTOR were measured using the western blotting. Results showed lower level of miR-145-3p and higher levels of PDK1 and mTOR in NSCLC tissues compared to the adjacent normal tissues. In vitro results showed that cell growth, cell motility, and chemotaxis were all inhibited in cells transfected with miR-145-3p and those transfected with siPDK. Additionally, dual luciferase reporter gene assay helped confirmed that PDK1 is a target of miR-145. Finally, levels of PDK1, mTOR, and phosphorylated-mTOR were lower in cells transfected with miR-145-3p as well as those with siPDK1. These findings indicate that miR-145-3p may inhibit cell growth, motility, and chemotaxis in NSCLC by targeting PDK1 through suppressing the mTOR pathway.

The elevated supersaturation of total dissolved gas (TDG) downstream of high-dam spills may lead to gas bubble disease or mortality in fish. Wind velocity is an important factor in accelerating the dissipation process of TDG. The accurate simulation and assessment of the dissipation process of supersaturated TDG are hampered by the scarcity of a quantitative description of the wind effect on the TDG dissipation process. The wind-driven experiments in this paper quantified the dissipation coefficients of supersaturated TDG under different wind velocities. A dimensionless ratio number, r , which represents the ratio between the dissipation coefficient with wind, Kw, and the coefficient without wind, K0, was introduced. A formula was developed to quantify the relationship between the dimensionless ratio number r and the wind velocity. The quantitative relationship was applied in a three-dimensional simulation to investigate the transportation and dissipation process of TDG in the wind-driven flow. The eddies generated by the wind-driven flow, which greatly contributed to the dissipation of TDG, and the spatial and temporal evolution of supersaturated TDG were obtained and were in good accordance with the measured data. The investigation provides a quantitative basis for the accurate prediction of the dissipation process of supersaturated TDG at different wind velocities.

The present study aims to investigate the effects of microRNA-218 (miR-218) on the proliferation, migration, invasion, and apoptosis of gastric cancer (GC) cells by targeting LIM and SH3 domain protein 1 (LASP1). The GC cells in the logarithmic phase were selected and divided into five groups: the blank group, negative control (NC) group, miR-218 inhibitors group, miR-218 inhibitors + siLASP1 group, and miR-218 mimics + siLASP1 group. The miR-218 expression in each group was also detected by qRT-PCR. The CCK8 assay, Transwell migration, and invasion assays and flow cytometry were performed to determine the effects of miR-218 on cell proliferation, migration, invasion, and apoptosis of GC cells. Western blotting was conducted to measure LASP1 protein expression in GC cells after transfection. The qRT-PCR revealed that the transfection of miR-218 mimics could upregulate the miR-218 expression, and the transfection of miR-218 inhibitors could downregulate the miR-218 expression in the GC cells. Compared with the blank and NC groups, the proliferation, migration, and invasion of GC cells were significantly reduced in the miR-218 mimics, miR-218 inhibitors + siLASP1, and miR-218 mimics + siLASP1 groups but enhanced in the miR-218 inhibitors group. Similarly, compared with the blank and NC groups, the cell apoptosis rates in the miR-218 mimics, miR-218 inhibitors + siLASP1, and the miR-218 mimics + siLASP1 groups were significantly increased, while the miR-218 inhibitors group had a lower apoptosis rate. In conclusion, these results indicate that miR-218 could inhibit the proliferation, migration, and invasion and promote apoptosis of GC cells by downregulating LASP1 expression.

Cadmium (Cd) is a biologically non-essential and toxic heavy metal leaking to the environment via natural emission or anthropogenic activities, thereby contaminating crops and threatening human health. Metallothioneins (MTs) are a group of metal-binding proteins playing critical roles in metal allocation and homeostasis. In this study, we identified a novel function of OsMT1e from rice plants. OsMT1e was dominantly expressed in roots at all developmental stages and, to less extent, expressed in leaves at vegetative and seed filling stages. OsMT1e was mainly targeted to the nucleus and substantially induced by Cd exposure. Expression of OsMT1e in a yeast Cd-sensitive strain ycf1 conferred cellular tolerance to Cd, even though the ycf1 + OsMT1e cells accumulated more Cd than the control cells (ycf1 + pYES2). Both transgenic rice overexpressing (OX) and repressing OsMT1e by RNA interference (RNAi) were developed. Phenotypic analysis revealed that OsMT1e overexpression enhanced the rice growth concerning the increased shoot or root elongation, dry weight and chlorophyll contents, whereas the RNAi lines displayed a sensitive growth phenotype compared to wild-type. Assessment with 0.5, 2 and 10 μM Cd for two weeks revealed that the RNAi lines accumulated less Cd, while the OX lines had an increased Cd accumulation in root and shoot tissues. The contrasting Cd accumulation phenotypes between the OX and RNAi lines were further confirmed by a long-term study with 0.5 μM Cd for one month. Overall, the study unveiled a new function of OsMT1e in rice, which can be potentially used for engineering genotypes for phytoremediation or minimizing Cd in rice crops.

An association between genetic polymorphisms in encoding X-ray repair cross complementing group 1 (XRCC1) and encoding xeroderma pigmentosum group D (XPD) and risks of non-small-cell lung cancer (NSCLC) in East Chinese Han population has been observed. Herein we hypothesized that genetic polymorphisms in these two DNA repair genes are likely to be important in the NSCLC in Chinese nonsmoking female patients. We recruited 327 nonsmoking female patients with NSCLC and 342 individuals with benign lung diseases or healthy controls. Genotype frequencies of XRCC1 T-77C, Arg194Trp, Arg280His and Arg399Gln, Pro206Pro, and XPD Asp312Asn and Lys751Gln were calculated after Polymerase Chain Reaction amplification and sequencing. Generalized multifactor dimensionality reduction (GMDR) was used to detect the interactive effect of XRCC1 and XPD gene polymorphisms. The ratio of cooking oil mist exposure history and soot exposure history, and the gene frequencies of XRCC1 T-77C TC + CC, XRCC1 AG + GG, XRCC1 399Gln/Gln, and XPD 751Gln/Gln were higher in female patients with NSCLC than those with benign lung diseases or healthy controls. The haplotypes of XRCC1 T-Arg-Arg-Gln and XRCC1 C-Arg-Arg-Arg were positively associated with the NSCLC occurrence in nonsmoking female patients. GMDR discovered that there was an interactive model of XRCC1 and XPD genes in multiple gene loci. Logistic regression analysis showed that XRCC1 T-77C, XRCC1 Pro206Pro polymorphism, cooking oil mist and soot exposure history and tumor-node-metastasis (TNM) stage were related to NSCLC occurrence for nonsmoking female patients. Taken together, XRCC1 and XPD polymorphisms, cooking oil mist, and soot exposure history may be interactively correlated with NSCLC incidence for nonsmoking female patients.

Microtubule-targeted drugs play a critical role in various types of cancer therapy worldwide. In our study, a series of novel indole derivatives containing a sulfonamide scaffold were designed, synthesized and biologically evaluated as potential tubulin polymerization inhibitors. Among them, compound 18 displayed the most potent inhibitory effect on antiproliferative activity against four types of human cancer cell lines (IC50 values of 0.24–0.59 μM) and tubulin assembly (IC50 = 1.82 μM). Further investigation demonstrated that compound 18 is a potent inducer of apoptosis in HeLa cells and could cause cell arrest in the G2/M phase of the cell cycle. Molecular docking and confocal microscopy assay results indicate that compound 18 could bind tightly to the colchicine binding site of tubulin and act as a tubulin polymerization inhibitor. A 3D-QSAR model was also built to provide more information which could be applied to the design of new molecules with more potent tubulin inhibitory activity.

The impacts of a low temperature economizer (LTE) on mercury removal across an electrostatic precipitator and influence of load variation on mercury conversion over selective catalytic reduction (SCR) catalysts were determined at two coal-fired boilers. When the LTE was on, the total and elemental mercury removal efficiency increased by 42.87% and 18.85%, respectively, due to the improvement of adsorption and oxidation capacity of the fly ash at lower temperature. Mercury speciation at the inlet and outlet of the SCR system were analyzed, and the impacts of load variation and catalyst aging on Hg0 conversion were discussed. The variable loads resulted in simultaneous changes of the gas hourly space velocity, the ambient temperature, and the oxygen content. The results showed the load ratio was significant for Hg0 conversion by the SCR catalysts and load reduction benefitted Hg0 conversion. When the load ratios were 100%, 75% and 60%, the Hg0 conversion were 61.78%, 65.71% and 72.12%, respectively. Moreover, Hg0 conversion was more significantly affected by the catalyst aging than NOx reduction. Among the three factors, the most important one is the flue gas temperature based on the grey relational analysis.

Ceramic coatings were prepared on the surface of AZ91D magnesium alloy by microarc oxidation technology. The effects of different voltages on morphology, phase composition and thickness of the coatings were characterized by SEM and XRD. The corrosion resistance of the coatings was measured by electrochemical workstation. Results indicated that the microarc oxidation coatings prepared in sodium silicate electrolyte exhibited porous surface and mainly comprised MgO, Mg2SiO4 and a small amount of MgAl2O4. The thickness of the oxide coatings increased rapidly with the increase of voltage. The coating prepared at 400V voltage had good electrochemical corrosion resistance in 3.5wt% NaCl solution.

This case-control study investigated the association of single nucleotide polymorphisms in the PTPN1 gene with susceptibility to esophageal squamous cell carcinoma (ESCC) in Inner Mongolia, China.A total of 302 patients living in Inner Mongolia China who were pathologically diagnosed with ESCC between April 2012 and 2016 were selected for the ESCC group; 373 healthy individuals were selected for the control group. The rs2904268 CG, rs2230605 AG, and rs16995309 CT polymorphisms in the PTPN1 gene were detected by bidirectional polymerase chain reaction amplification of specific alleles. The haplotype frequencies were analyzed by SHEsis software. Binary logistic regression analysis was conducted to analyze risk factors associated with ESCC.Statistical differences between the ESCC and control groups were observed for history of smoking, drinking, and poor eating habits (all p 0.05). Both the rs2904268 CG CG and GG genotype frequencies were markedly higher in the ESCC group relative to the control group (both p 0.05). However, the genotype frequencies of rs2230605 AG and rs16995309 CT were similar between the ESCC and control groups (all p 0.05). Compared with the control group, the ESCC group had notably elevated frequencies of the GGC and GAT haplotypes and significantly reduced frequencies of CGC and GGT haplotypes (all p 0.05). A history of smoking, drinking, poor eating habits, the rs2904268 CG CG+GG genotypes, and the GAT haplotype were all identified as risk factors for ESCC (all p 0.05).These results indicated that the PTPN1 gene polymorphism rs2904268 is associated with susceptibility to ESCC in Inner Mongolia.

In order to overcome the shortcomings of the single chaotic encryption and the problems of simple structure and low security, the hyperchaotic colour image encryption algorithm based on DNA sequences is proposed. Firstly, the colour images are layered and encoded by DNA, then, the hyper-chaotic sequence is used to scramble the DNA matrix, the hyper-chaos is used to generate natural DNA matrix and DNA addition operations is dynamically selected to perform the DNA rule operations, finally, the cipher-text image is obtained. The performance test of the cipher text image shows that the gray scale distribution of the histogram is relatively uniform; the test values of the related index parameters NPCR and UACI are 99.65% and 33.51%, respectively, which are close to the theoretical value through simulation experiments. The algorithm can effectively improve various anti-attack capabilities of the encryption system and has high security.

Purpose:To evaluate the epigenetic risk linked to assisted reproductive technology (ART) at single-embryo level by analyzing the methylation status of imprinted H19, PEG1, and KvDMR1 in human preimplantation embryos.Methods:A total of 254 human day 3 embryos produced by ART procedures were collected. All embryos were normally fertilized but unsuitable for transfer or freezing due to poor quality. Pyrosequencing with confirmatory routine bisulfite sequencing were used to determine the DNA methylation patterns of H19 differentially methylated region (DMR) in 63 embryos, PEG1 DMR in 65 embryos, and KvDMR1 in 67 embryos.Results:Aberrant methylation patterns were found in 8.0% embryos at H19 DMR, 16.9% embryos at PEG1 DMR, and 10.4% embryos at KvDMR1. No methylation errors were found in corresponding sperm samples.Conclusions:We hypothesized that the use of poor-quality embryos may increase the risk of imprinting defects because they might have methylation errors.

The elevated supersaturation of total dissolved gas (TDG) downstream of a high-dam spill has deleterious effects on fish in a large range. A one-dimensional (1-D) longitudinal model is optimal for the prediction of supersaturated TDG dissipation over a long distance. The key issue of the model is to determine the dissipation coefficient accurately. In agreement with field observations and experiment data, dimensional analysis and regression were performed to propose a formula for estimating the dissipation coefficient of supersaturated TDG in various rivers and reservoirs, and it involves the effects of the turbulence intensity, the hydro-pressure and the solid-liquid interface. The friction velocity, water depth, hydraulic radius and Froude number are independent variables in the formula which are easy to determine in practical applications. The 1-D longitudinal model is implemented to calculate the dissipation of TDG in a reach of the Jinsha River. Good agreement is found between the calculated results and field data for both the dissipation coefficient and the dissipation process.

Cloud computing as a new network technology has been widely used in various fields, a important role of cloud computing is the storage and management for massive information data, therefore the cloud computing provides a good platform for data mining. This paper first describes simply on cloud computing, and then expounds and analyzes data mining application which based on cloud computing, finally introduces the data mining application based on cloud computing platform.

Node positioning technology in the Wireless sensor network is a very important issue. This article introduces the ant colony algorithm and its characteristics. It is a good solution to the static and dynamic portfolio optimization problem. The node positioning method in the wireless sensor network can be divided into two categories based ranging and no ranging. In order to get a higher positioning accuracy, and a further improvement the positioning accuracy, this paper introduces the ant colony optimization algorithm based on the first positioning method of the feasibility. The experimental results demonstrate the decrease of the number of transmitting beacon and the optimal mobile path after being optimized.

Wireless sensor networks integrated four technologies including sensor, embedded computing, network technology and wireless communication. It is a new type of non-infrastructure wireless network. In this paper, a data fusion method has been brought forward based on wireless sensor networks, and through an algorithm simulation test, It is proved that the algorithm is effective to reduce the energy consumption of the network, and extend the lifetime of the network.

Lightweight sludge ceramsite was manufactured using dewatered sludge from municipal sewage treatment plants as the main raw material, complemented by fly ash and clay ingredients. The results indicate that the sintering temperature on the ceramsite’s performance impacts the most. Sludge ratio has certain influence on the bulk density of sludge ceramsite. The ratio of sludge in the experiment can be up to 80% and meet the requirements of the light weight

Objective To evaluate the epigenetic risk linked to assisted reproductive technology at oocyte level by analyzing methylation status of imprinted H19, PEG1 and KvDMR1 in human MII oocytes rescue-matured from MI/GV oocytes after long agonist stimulation. Study design 580 MI/GV oocytes from 275 patients receiving an ICSI procedure were additionally cultured for 24 h, and 221 rescue-matured MII oocytes were obtained. Pyrosequencing with confirmatory routine bisulfite sequencing were used to determine the methylation status of H19 DMR in 35 oocytes, PEG1 DMR in 47 oocytes, and KvDMR1 in 34 oocytes. Results Abnormal methylation status were found in 22.9% oocytes at H19 DMR, 17.0% oocytes at PEG1 DMR and 8.8% oocytes at KvDMR1 . Conclusion We hypothesize that the use of MII-rescue oocytes may increase the risk of imprinting defects because they might not have completed full imprinting programme.

This paper expounds the ceramisite concrete fine characteristic, the development history, present situation and the existing problems. Using orthogonal design to study on the internal and external factors of compressive strength and dry apparent density and specific strength of ceramisite concrete. At the same time, using the professional Image analysis software of Image-pro plus to analysis and evaluate the uniformity of ceramisite concrete and its influencing factors were discussed.

The credit risk is introduced into the pricing model of convertible bond in this paper. The main results of paper have three aspects: (1) By modifying the dynamic motion of stock, a defaultable stock process is obtained in neutral risk measure, then the pricing model of convertible bond with finite maturity and credit is proposed. (2) The defaultable binary tree algorithm is proposed, and the convergence of algorithm is proved.

Because MOSS (Microsoft Office SharePoint Server) doesn’t support the association between two heterogeneous entities, we propose a clustering algorithm called MHEA. This algorithm can mine the associations among heterogeneous entities and output entity association matrices. It is based on Extended Name Vectors which is the result of ENV clustering algorithm. ENV is based on k-means, but it selects initial centroid of each clustering according to the identifiers of entities, and the value of k is dynamically changeable in the process of ENV, consequently decrease the iterative times. The accuracy and effectiveness of our proposal have been demonstrated through the performance study using both real and synthetic data sets.

Assisted reproductive technology (ART) including in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) have been shown to be associated with abnormal genomic imprinting, thus increasing the incidence of imprinting disorders such as Beckwith-Wiedemann syndrome (BWS) and Angelman syndrome (AS) in ART-conceived children. Furthermore, a recent study described abnormal DNA methylation in clinically normal children conceived by ART. However, data from different studies are conflicting or inconclusive. This study examined DNA methylation patterns of multiple imprinted genes in children born after ART to primarily evaluate the impact of ART on genomic imprinting. A total of 101 newborns conceived by ART (40 ICSI and 61 IVF) and 60 naturally conceived newborns were involved in our study. After obtaining the approval of the Institutional Ethics Committee, umbilical cord blood was collected from each infant. Genomic DNA was isolated from each blood sample and treated using sodium bisulfite. Subsequently, using methylation-specific PCR (MS-PCR), we analyzed six differentially methylated regions (DMRs) including KvDMR1, SNRPN, MEST, MEG3, TNDM and XIST. Meanwhile, information regarding twin pregnancies, gestational age, and birth weight of the neonates was documented. None of the cases presented with phenotypic abnormalities. Children conceived by ART were more likely to have low birth weight and to be born before term, compared with children conceived spontaneously. However, 7 months to 3 years of clinical follow-up showed that none of the children had clinical symptoms of any imprinting diseases. Furthermore, the MS-PCR results showed that all 161 children had normal DNA methylation patterns at six DMRs despite the different mode of conception. Our data did not indicate a higher risk of DNA-methylation defects in children born after ART. However, further studies using quantitative methods are needed to confirm these results.

Convertible bond gives holder the right to choose a conversion strategy to maximize the bond value, and issuer also has the right to minimize the bond value in order to maximize equity value. When there is default occurring, conversion and calling strategies are invalid. In the framework of reduced form model, we reduce the price of convertible bond to variational inequalities, and the coefficients of variational inequalities are unbounded at the original point. Then the existence and uniqueness of variational inequality are proven. Finally, we prove that the conversion area, the calling area and the holding area are connected subsets of the state space.

PC-1(Prostate and colon gene 1) gene belongs to TPD52 (Tumor Protein D52) gene family. The expression of PC-1 is found to promote androgen-independent progression. This study was conducted to assess the mechnism of promotion of androgen-independent progression in PC-1 gene. The c-myc gene expression was tested by RT-PCR and Western blotting analyses in the LNCaP-pc-1 and LNCaP-zero cell line. After separation of cytoplasm and nulear proteins of the LNCaP-pc-1 and LNCaP-zero cell line, the beta-catenin protein was detected by Western blotting. C4-2 cell line was used to examine the effects of 10058-F4 on the PC-1 gene expression. The results of RT-PCR and Western blotting indicated that PC-1 enhanced c-myc gene expression in prostate cancer cells, PC-1 was also found to enhance beta-catenin expression in nuclear. Furthermore, a small-molecule c-Myc inhibitor, 10058-F4 represses PC-1 gene expression in C4-2 cell line. Our findings suggest that PC-1 enhances c-myc gene expression in prostate cancer cells through the Wnt/beta-catenin pathway. Meanwhile, c-myc plays a feed-forward role in enhancing PC-1 driven c-myc gene expression, and promotes prostate an-drogen-independent progression.

An efficient, clean and facile protocol for the Knoevenagel condensation of aromatic aldehydes with active methylene compounds catalysed by task specific ionic liquid 1,8-diazabicyclo[5.4.0]-undec-7-en-8-ium acetate in an aqueous medium has been developed. The reactions proceed at room temperature and high to excellent yields were afforded. The workup procedure is very simple. The ionic liquid could be recycled for 10 runs without noticeably loss of its catalytic activity.

Lipases are the most attractive enzymes for use in organic chemical processes. In our previous studies, a lipase from Rhizopus chinensis CCTCC M20102 was found to have very high ability of esterification of short-chain fatty acids with ethanol. In this study, we reported the cloning and expression of the lipase gene from R. chinensis in Pichia pastoris and characterization of the recombinant lipase. The lipase gene without its signal sequence were cloned downstream to the alpha-mating factor signal and expressed in P. pastoris GS115 under the control of AOX1 promoter. In the induction phase, two bands of 37 kDa and 30 kDa proteins could be observed. The amino-terminal analysis showed that the 37-kDa protein was the mature lipase (30 kDa) attached with 27 amino acid of the carboxy-terminal part of the prosequence (r27RCL). The pH and temperature optimum of r27RCL and mRCL were pH 8.5 and 40 °C, and pH 8 and 35 °C, respectively. The stability, reaction kinetics and effects of metal ions and other reagents were also determined. The chain length specificity of r27RCL and mRCL showed highest activity toward p -nitrophenyl hexanoate or glyceryl tricaproate (C6) and p -nitrophenyl acetate or glyceryl triacetate (C2), respectively. This property is quite rare among lipases and gives this new lipase great potential for use in the field of biocatalysis.

In this paper, taking into account the shortcomings of wireless sensor networks, LEACH algorithm, the algorithm has been improved on this basis. The design of the E-LEACH algorithm, which extend the network lifetime, improve node energy utilization, and the effectiveness of this algorithm through simulation experiments.

Climate change is one of the most significant factors for the migration of the suitable planted areas of maize. The hyper-resolution data of daily mean precipitation, temperature, soil, and topography that supported by Institute of Soil Science and National Climate Center were collected to assess the effect of climate change on migration of the suitable planted maize areas, and it provides a foundation for macro-management decisions of agriculture. Climate data mentioned above was simulated by regional climate model, which showed the experiments of the twentieth century and the forecast tests of twenty-first century. Based on the factors above, relevant criteria, suitability levels, and their weights for each factor were defined. And the database of climate and soil was established. In this article, we use the multi-criteria evaluation (MCE) approach based on GIS to identify the suitable planting areas for maize in China. The results demonstrated that the most suitable degrees of active accumulated temperature, maximum and minimum temperature in growth period moving north dramatically, and the precipitation moving slightly in the future, which will lead to the planting areas of maize moving northward, and the areas were increased by 4.61 × 105 km2.