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Background/Objectives Carbonic anhydrase 1 (CA1)/kininogen and selenoprotein W (SelW)/14-3-3η signal transduction orchestrate oxidative stress, which can also be regulated by nitric oxide (NO). The mutated caveolin-1 (Cav-1F92A) gene may enhance NO production. This study explored the effect of Cav-1F92A-modified rat bone marrow mesenchymal stem cells (rBMSC/Cav-1F92A) on oxidative stress regulation through CA1/kininogen and SelW/14-3-3η signal transduction in a rat model of monocrotaline- (MCT-) induced pulmonary arterial hypertension (PAH). Method PAH was induced in rats through the subcutaneous injection of MCT. Next, rBMSC/Vector (negative control), rBMSC/Cav-1, rBMSC/Cav-1F92A, or rBMSC/Cav-1F92A+L-NAME were administered to the rats. Changes in pulmonary hemodynamic and vascular morphometry and oxidative stress levels were evaluated. CA1/kininogen and SelW/14-3-3η signal transduction, endothelial nitric oxide synthase (eNOS) dimerization, and eNOS/NO/sGC/cGMP pathway changes were determined through real-time polymerase chain reaction, Western blot, or immunohistochemical analyses. Results In MCT-induced PAH rats, rBMSC/Cav-1F92A treatment reduced right ventricular systolic pressure, vascular stenosis, and oxidative stress; downregulated CA1/kininogen signal transduction; upregulated SelW/14-3-3η signal transduction; and reactivated the NO pathway. Conclusions In a rat model of MCT-induced PAH, rBMSC/Cav-1F92A reduced oxidative stress by regulating CA1/kininogen and SelW/14-3-3η signal transduction.

Pulmonary arterial hypertension (PAH) is characterised by remodelling in vascular smooth muscles, and switching from contractile (differentiated) to synthetic (dedifferentiated) phenotype. This study aimed to investigate the effect of a mutated caveolin-1 (Cav1Human pulmonary smooth muscle cells (HPASMCs) were treated with monocrotaline (MCT,1μM), and co-cultured with Cav1Cav1rBMSCs/Cav1

This thesis regards black tea as the analysis target. Basing on the analysis of plenty of black tea added with different food additives, the visual spectral analysis technology is used to scan the absorption spectrum of the tea, and the MATLAB software is applied to optimize the data, then ultimately we can get the absorption spectrum curves of several groups of tea added with different food additives. Through the analysis and comparison of the absorption spectrum curves of the tea before and after added with food additives, we can observe the effects of different additives on the tea intuitively. This study provides a new way for analyzing the effects of food additives on black tea and the composition of tea beverages with the usage of visual spectral analysis technology.

A kind of different thermal radiation source which consists of photonic crystals (PCs) containing negative-index materials (NIM) coated atop SiC substrate was investigated theoretically by a transfer matrix method. The photonic crystals were inserted into a defect layer，and the wavelength range of them was tuned to approximately match with the wavelength range of the phonon absorption band of polar material SiC. So the structure can only radiate the light with same wavelength as the gap defect mode. By the calculation, the structure shows very sharp emissivity peak in a narrow wavelength band. The position of the emissivity peak can be effectively controlled by tuning the thickness of the defect layer. Due to the novel characteristics of the defect mode of gap, this kind thermal emission is predicated to have promising radiative properties.

Composite material has extensive application in aeronautics, astronautics and other fields due to the properties of high intensity, light weight and anti-fatigue. So controlling and rapid repairing of damaged composite material has become one of the most popular research subjects. In this paper, the common composite material for aircraft is used as test-object; The light wave of 675nm transmitted by optical fiber is used as repairing light source; special repairable reagent which can be stimulated by the light is adopted, and by the light measure technology, the effect of the vibration property in the composite material is studied by light repairing after the damage of the composite material. Based on this study, the results and analysis are shown. The research result shows that this technology is feasible in the repair of composite material and offers an experimental basis on instant test and controlling for composite material.

Composites, as a type of new material with typical characteristics, has been applied in aerospace, space, buildings, bridges and many other areas. Rapid repair from damaged composites has become a hot research project. This paper proposed a technique of light repair on composites, which is often used as specimen in aerospace composites. The research has taken advantage of modern optical measurement to study the influence of light remediation on the structural bending of composites, put forth a law of deflection variation, and proven that light remediation is feasible. The result of this research has laid the foundation that light remediation can be further studied and applied in many fields.

Corticosteroids are an option in the treatment of community-acquired pneumonia (CAP). However, the benefits and adverse effects of corticosteroids, especially in severe CAP, have not been well assessed.PubMed, Embase, and Cochrane library databases from inception to May 2015 were searched. Randomized controlled trials (RCTs) and cohort studies that evaluated use of corticosteroids in adult patients with CAP were included. The quality of outcomes was evaluated using Grading of Recommendations Assessment, Development and Evaluation methodology. The Mantel-Haenszel method with random-effects modeling was used to calculate pooled relative risks (RRs) and 95% CIs.Nine eligible RCTs (1,667 patients) and six cohort studies (4,095 patients) were identified. The mean corticosteroid dose and treatment duration were 30 mg/day methylprednisolone for 7 days. Corticosteroids did not have a statistically significant effect on mortality (RR, 0.72; 95% CI, 0.43-1.21; evidence rank, low) in patients with CAP and patients with severe CAP (RCTs: RR, 0.72; 95% CI, 0.43-1.21; evidence rank, low; cohort studies: RR, 1.00; 95% CI, 0.86-1.17 ). Corticosteroids treatment was associated with a decreased risk of ARDS (RR, 0.21; 95% CI, 0.08-0.59) and may reduce lengths of hospital and ICU stay, duration of IV antibiotic treatment, and time to clinical stability. Corticosteroids were not associated with increased rates of adverse events.Short-term treatment with corticosteroids is safe and may reduce the risk of ARDS, shortening the length of the disease in patients with CAP.

The fluorescence spectrum was studied for the chlorothalonil (0.2928 mg x mL(-1)) using spectrofluorophotometer. The experiment results showed that the characteristic peaks (352 and 366 nm) are found in the spectrum of chlorothalonil standard solution when the excitation wavelength is 320 nm. And it was found that the shoulder peak gradually disappeared at 366 nm, while the fluorescence peak is stable at 352 nm with the decline of the solution concentration The exponential functional relationship between the concentration of chlorothalonil and fluorescence intensity at 352 nm was obtained, and its correlation coefficient is 0.999. The experimental results are consistent with the theoretical formula about fluorescence intensity and concentration The prediction model functions were also obtained through the liner fitting to the chlorothalonil solution of low concentration, and the correlation coefficient is 0. 995. The limit of detection (LOD) is 0.0188 microg x mL(-1), the limit of quantification (LOQ) is 0.0627 microg x mL(-1), and the linear range is 0.0627-28.45 microg x mL(-1). And fluorescence spectra were studied for the mixed system of astragalus, medlar and chlorothalonil. It was found that the fluorescence intensity of chlorothalonil solution is all declined with the addition of two kinds of Chinese Herbal Medicines, which indicates that there is an interaction between them. The decay rate of fluorescence intensity was obtained which is 88.5% and 99.7%, respectively. Then the model functions were established between fluorescence intensity and the volume of addition, and the correlation coefficient is 0.994 and 0.997, respectively. This study provides the experimental foundation for the detection of chlorothalonil residues using fluorescence spectrum. It is shown that it is possible to detect pesticide residues of chlorothalonil using fluorescence spectra directly, and the relevant parameter value satisfied the requirement of testing standard. Therefore there is an important value for further detecting the pesticide residues in fruit juice using fluorescence spectrum. It was also found that the fluorescence intensity of chlorothalonil is decreased with the addition of astragalus or medlar, which provides the new research approach to studying the pesticide degradation using medicinal and edible Chinese Herbal Medicines.

Oxidative stress plays a role in diabetic retinopathy. L-Carnitine is an endogenous mitochondrial membrane compound.To elucidate the protective effects of L-carnitine on high glucose-induced oxidative stress in retinal ganglion cells (RGCs).Hoechst 33258 staining was used to estimate cell loss. Mitochondrial function was predicted by mitochondrial membrane potential (ΔΨm) measurement. The expression of apoptosis-related protein was measured by Western blotting. Assays for reactive oxygen species (ROS) accumulation, lipid peroxidation, total antioxidative capacity (T-AOC) and antioxidant defense enzymes were completed to explain the antioxidative capacity of L-carnitine.L-Carnitine (12 h) inhibited high glucose-mediated cell loss and restored mitochondrial function including a reversion of ΔΨm loss and cytochrome c release. Cell apoptosis triggered by high glucose was also inhibited by L-carnitine, characterized by the downregulation of caspase-9, caspase-3 and Bax/Bcl-2. Furthermore, L-carnitine inhibited high glucose-induced ROS production and lipid peroxidation and promoted endogenous antioxidant defense components including superoxide dismutase, glutathione peroxidase, catalase and T-AOC in a concentration-dependent manner.L-Carnitine may protect RGCs from high glucose-induced injury through the inhibition of oxidative damage, mitochondrial dysfunction and, ultimately, cell apoptosis.

Intravenous infusion of prostaglandin E1 (PGE1) has been used to treat pulmonary arterial hypertension (PAH); however, the efficacy and safety of inhaled PGE1 is unclear.To investigate the effect of inhaled PGE1 on PAH following corrective surgery for congenital heart disease.Sixty patients with postoperative residual PAH following corrective surgery for congenital heart disease were randomly assigned to a control group, a PGE1 infusion group (intravenous PGE1 infusion; 30 ng/kg/min daily for 10 days) or a PGE1 inhalation group (100 μg nebulized PGE1 every 8 h for 10 days). Systolic blood pressure, mean pulmonary arterial pressure, arterial oxygen pressure, oxygen saturation and serum endothelin-1 level were measured before and after the treatment.At the end of the study, the mean pulmonary arterial pressure in the two PGE1 groups were lower than in the control group (P0.01), whereas the mean arterial oxygen pressure was higher (P0.01). Compared with the PGE1 infusion group, the mean pulmonary arterial pressure in the PGE1 inhalation group was lower (P0.01) whereas the arterial oxygen pressure was higher (P0.01). The mean endothelin-1 levels in the two PGE1 groups were lower than in the control group (P0.01), but there was no statistically significant difference in endothelin-1 levels between the PGE1 inhalation and infusion groups (P0.05).In pediatric patients with PAH, PGE1 inhalation was associated with a reduction in pulmonary arterial pressure and improvement in arterial blood oxygen levels.

Absract Chronic heart failure is one of the most common heart diseases and is a leading cause of morbidity and mortality. There has been a rapid growth in knowledge and understanding of the pathogenesis of chronic heart failure in recent years. Tumor necrosis factor (TNF-α), a pro-inflammatory cytokine, is found to play an important role on the development and progression of heart failure. Recent evidence suggests that testosterone improves cardiac function and clinical symptoms in patients with chronic heart failure. Recent experimental studies show that testosterone suppresses the biosynthesis of TNF-α while increasing the levels of anti-inflammatory faction, interleukin-10. Therefore, we hypothesize that testosterone may also suppress the biosynthesis of TNF-α and re-balancing the pro- and anti-inflammatory mechanisms in human hearts, leading to significant improvement in cardiac function and symptoms in patients with chronic heart failure.

To investigate the effect of ischemic postconditioning on the expression of matrix metalloproteinase (MMP)-2 during ischemia-reperfusion of myocardium in a rabbit model.Thirty-six male New Zealand white rabbits were randomly divided into sham, ischemia-reperfusion and ischemic postconditioning groups. Myocardial ischemia-reperfusion was created by ligating the left anterior descending coronary artery for 30 min followed by 3 h of reperfusion. Myocardial infarction sizes were determined by dual staining with triphenyltetrazolium chloride and trypan blue. Plasma levels of MMP-2 were measured using ELISA. Myocardial MMP-2 messenger RNA was analyzed by reverse transcription polymerase chain reaction.The mean (± SD) infarct size in the ischemic postconditioning group was significantly smaller compared with the ischemia-reperfusion group (37.1±3.8% versus 57.5±1.9%; P=0.02). The incidence of ventricular tachycardia in the ischemic postconditioning group was also lower than in the ischemia-reperfusion group (8.5% versus 75%; P=0.003). MMP-2 messenger RNA expression in the ischemic postconditioning group was significantly lower compared with the ischemia-reperfusion group (0.4944±0.0476 versus 0.6989±0.0694; P=0.02).Ischemic postconditioning reduces myocardial ischemia-reperfusion injury, possibly by inhibiting the expression of MMP-2.

Peripartum cardiomyopathy (PPCM) is a potentially fatal form of heart failure and the recognition of its risk factors is important for prevention and treatment.To explore the clinical characteristics and the risk factors for PPCM.Echocardiographic was used to examine the left ventricular ejection fraction (LVEF). Blood level of troponin I (cTNI), high sensitive C-reaction protein (hs-CRP), NT-proBNP was measured. All PPCM occurred within weeks following delivery.Fifty-two PPCM patients and 52 normal delivery subjects (control group) were included in this study. Compared with the control group, PPCM patients were older, with a higher level of blood pressure, and a higher rate of suspected respiratory infection. The level of leucocytes, hs-CRP, cTNI and NT-proBNP in PPCM patients were higher than in the control. Multivariate logistic regression analysis showed that elevated plasma hs-CRP (OR =1.86, p0.05), respiratory infection (OR = 2.87, p0.01), and hypertension (OR =1.68, p0.05) were independent risk factors for PPCM. During the follow up of 21.6±5.4 d, one patient (1.9%) died probably of heart failure but other patients remained well.Hypertension, respiratory infection, and elevated plasma hs-CRP seem to be associated with the pathogenesis of peripartum cardiomyopathy in this patient population.

The outcome of surgical treatment of patients with intrahepatic cholangiocarcinoma (ICC) is poor. This study was designed to analyze prognostic factors following surgical treatment for ICC.A structured telephone interview was conducted in 132 patients who were surgically treated for ICC. Fifteen clinical and pathological factors that may influence post-operative survival were analyzed by using Cox proportional hazards model.The accumulative 1-, 3-, 5-year survival rate of the 132 patients was 51.3%, 21.6%, and 11.8% respectively. The mean survival time in patients with elevated serum carbohydrate antigen (CA) 19-9 at the time of the operation was shorter than in patients with normal serum CA19-9 (9.6 +/- 24.7 vs 16.1 +/- 6 months, P0.01). The median survival time in patients with well-differentiated carcinoma was longer than in those with poorly differentiated ICC (23.9 +/- 7.8 vs. 11.2 +/- 5.0 months, P0.01). Patients who were treated with hepatectomy and lymph node dissection had a longer survival time than those treated with hepatectomy only (16.0 +/- 5.8 vs 10.2 +/- 3.6 months, P0.01). Multivariate analysis showed that mode of surgical treatment, lymph node metastasis, serum level of CA 19-9 and pathological differentiation grade of ICC predicted postoperative survival.Hepatectomy with lymph node dissection is associated with an improved survival for patients with ICC. This strategy may be recommended for the surgical treatment of ICC.

More than 50% of patients with heart failure die from sudden cardiac death as a result of malignant arrhythmia. T wave alternans (TWA) is a convenient, noninvasive, and inexpensive testing modality, with a higher sensitivity and specificity for sudden cardiac death. Its prediction value for malignant arrhythmia may even exceed electrophysiologic study. Generally, the algorithms of TWA can be divided into frequency-domain and time-domain methods, and the latter has a stronger anti-interference ability. So far, a unified measuring formula and diagnostic criteria about TWA measurements have been created. Large clinical studies in recent years strongly suggest that TWA can predict sudden cardiac death, which can be used as a guide for the implanting of implantable cardioverter-defibrillator. This article reviews the current literature on recording techniques and clinical implications of TWA.

To evaluate the effects of educational and psychological intervention on the quality of life and psychological status of patients with asthma.Asthmatic patients were randomized to study (n = 228) and control (n = 146) groups. Both groups received conventional pharmacotherapy for asthma. The study group also received education and psychological counseling. We assessed quality of life, mood states, and asthma knowledge before and after the educational and psychological interventions.The study group's mean quality-of-life score was higher than that of the control group immediately after the intervention (130.2 +/- 25.1 vs 111.6 +/- 27.4, P.001) and 3 months after the intervention (144.4 +/- 16.9 vs 121.5 +/- 25.6, P.001). The study group's mean asthma-knowledge score was higher immediately after the intervention (9.0 +/- 1.2 vs 7.5 +/- 1.8, P.001) and 3 months after the intervention (9.4 +/- 0.8 vs 7.5 +/- 1.5, P.001). Two weeks after the intervention the mean increment of the asthma-knowledge score in the study group was greater than that in the control group (1.8 +/- 1.6 vs 0.6 +/- 1.7, P = or.01). The study group's mean Profile of Mood States score was lower than that of the control group immediately after the intervention (12.0 +/- 18.5 vs 23.0 +/- 22.7, P.001) and 3 months following the intervention (10.2 +/- 7.5 vs 22.8 +/- 11.4, P.001).Education and psychological counseling improves the quality of life and alleviates the psychological distress in patients with asthma. These interventions also enhance patient's understanding of this chronic disease.

The spectral analysis technology applied to some blood diseases diagnosis is convenient and speedy. The experimental result shows that the line type and peak value of the fluorescence spectra of serum excited by ultraviolet radiation with different wavelength remain the same, but the fluorescence peak value changes with the wavelength of the blazed light. The present paper studied how the serum fluorescence spectrum changes with the serum concentration, adopting Shimadzu Corporation (Japan) fluorescence photometer RF5301, and provided the experiment basis for disease diagnosis by hemanalysis. The experimental result shows that the fluorescence spectrum of serum is different under the excitation of different monochromatic light, the relative fluorescence intensity of serum increases with the serum concentration when excitated with 220, 230 and 310-420 nm monochromatic light, but the serum fluorescence intensity decreases while the serum concentration increases when the excitation monochromatic light is between 240 nm and 420 nm. The experimental research found that the serum concentration quenching and absorption effect is not obvious when the experimental sample is excitated by 220, 230 and 310 to 420 nm monochromatic light, and the fluorescence stimulation plays the main role. The serum concentration quenching and absorbing effect is obvious, so the fluorescence intensity becomes weaker and weaker with serum concentration increasing when the sample is excitated by 240 nm to 300 nm monochromatic light. This work provides the experimental basis for the choice of serum concentration in the study of serum fluorescence spectrum.

This study was designed to investigate whether there was any difference in blood pressure (BP) readings between sitting and supine positions in diabetic and non-diabetic subjects.BP in sitting and supine position was measured in 356 patients with type 2 diabetes (study group) and in 356 age- and sex-matched non-diabetic subjects (control group).The systolic and diastolic BP in the supine position was significantly higher than in the sitting position in both groups (P0.001). The BP increment in the supine position was similar in the study and control groups (P0.05). Multivariate logistic regression analysis of the study group showed that age was an independent predictor for the systolic pressure increment (P=0.001), whereas body mass index was an independent predictor for the increment in diastolic BP (P=0.04). The levels of sitting BP were inversely correlated with the pressure increments in the supine position in the diabetic patients (P0.001).In diabetic and non-diabetic subjects, BP in a supine position is higher than in the sitting position. In diabetic patients, age, body mass index and the levels of sitting BP seem to have significant impact on the pressure increment from a sitting to the supine position.

In the present paper the fluorescence spectra of the blood serum excited with different wavelength were measured with the fluorescence photometer RF5301 (SHIMADZU) made in Japan. The relationship between the fluorescence spectra of the serum and the wavelength of the excitation light was studied during the experiment. The experimental results show that the linetype and peak wavelength of the fluorescence spectra of serum excited by ultraviolet radiation with different wavelength are almost the same, and they do not depend on the excitation wavelength. But the fluorescence peak value changes with the excitation wavelength. There are two high intensity emission intervals in the fluorescence spectra. One of these is from about 300 nm to 410 nm, and the other is below 310 nmr The fluorescence spectra are mostly centralized in the first interval, and the wavelengths of fluorescence peaks were found around two locations: one is near 330 nm and the other is near 370 nm. At this time the strife phenomena occur. When the excitation wavelength is about 250 nm or higher, the fluorescence peak only occurs at 330 nm, and the optimal excitation wavelength is 300 nm. While the wavelength is greater than 320 nm, the fluorescence intensity of the first interval begins to fall, while that of the other augments. And at this time the peak of wavelength of fluorescence is 452 nm. This study provides the experimental foundations for advanced study and applications of the characteristics of fluorescence spectrum of blood serum, and also offers the references to the wavelength selection of excitation light in the application of the photo-induced fluorescence spectra diagnostic technology.

The present paper puts forward a method of disease diagnosis by using the technology of spectrum analysis of human blood serum. The generation mechanism of absorption spectrum is explained and the absorption spectra of the normal blood serum and the sick blood serum are listed from the experiments of absorption spectrometry. Though the value of absorbency of the sick blood serum is almost equal to that of the normal blood serum in the most absorption spectra, there are some differences around 278 nm in the absorption spectrum. The absorbency of the blood serum with hyperglycemia is greater than that of the normal blood serum at 285 nm in the spectrum, and besides, there comes a peak shift of absorption with hyperglycemia. In the absorption spectrum of the blood serum with hypercholesterolemia, there is a clear absorption peak at 414 nm. However there is not any peak at that wavelength in the absorption spectrum of the normal blood serum. Through comparing the characters of the spectrum, we can judge if the blood sample is or not, and this blood analysis is a new method for the diagnosis of disease. Compared with other methods of blood measurements, the method of absorption spectrum analysis of blood serum presented in this paper, is more convenient for measurement, simpler for analysis, and easier to popularize.

Blood plays an important role in the clinical diagnosis and treatment; many diseases may lead to changes in the blood composition or characteristic properties. Therefore, the analysis of blood sugar will be of great importance. The present paper studies the difference of fluorescence intensity stimulated by light wave between normal blood serum and hyperglycemia blood serum. The result shows that the average grey level of fluorescence image at 365 nm increases obviously with the changes in the concentration of glucose; however, the average grey changes little at 353, 369 and 405 nm. This shows that the main effect of serum glucose on the fluorescence intensity peak appears at 365 nm. Because the fluorescence intensity has something to do with the concentration of the fluorescence material, the blood glucose concentration influences directly the fluorescence intensity at 365 nm. Therefore, it is possible to judge blood sugar content, and to make sure whether the content exceeds the normal value or not by comparing the fluorescence intensity of the blood serum measured at 365 nm. This technique of optical measurement provides an experimental basis for the diagnosis of blood diseases.

To evaluate the efficacy of prolonged intra-aortic balloon pumping (IABP) support in patients with cardiogenic shock following acute myocardial infarction (AMI).Thirty-nine patients with cardiogenic shock after AMI were treated with percutaneous coronary intervention which was supported by IABP. After 72 hours of IABP, the patients who attained the criteria of IABP withdrawal were randomly divided into two groups. The control group ceased IABP whereas the study group continued IABP for additional seven days.After IABP, mean arterial pressure, cardiac index, left ventricle ejection fraction and arterial oxygen saturation were significantly elevated in all patients whereas pulmonary capillary wedge pressure and heart rate were decreased. The improvement of cardiac index, left ventricular ejection fraction and pulmonary capillary wedge pressure in the study group was greater than the control group (P0.05). After 12-month follow-up, the 6-min walking test and left ventricular ejection fraction in the study group were significantly higher than those of the control group (P0.05). No significant differences were noted between the two groups in the incidence ventricular aneurysm and mortality rate.Prolonged use of IABP for up to 10 days offers additional long term benefit in left ventricular function and exercise tolerance.

The analysis technique of fluorescence is adopted to study the intensity of excitation fluorescence of blood in the present paper. The theoretical analysis and differences of normal and abnormal blood (hyperglycemia, hyperlipemia) are presented. The theoretical analysis was proved by experiment results. It was discovered that blood sugar consistency has an effect on blood fluorescence. In other words, with the same excitation wavelength and with blood sugar consistency increasing, the fluorescence intensity increases gradually. It is obvious that blood sugar is also a kind of fluorescein, and its consistency has an effect on fluorescence intensity, which is identical with the theoretical analysis, indicating that the experiment is successful, and it is possible to distinguish blood sugar consistency by comparing the fluorescence intensity in blood. It was also discovered that the higher the cholesterin content, the more intense the fluorescence. When excitation wavelength is especially 435 nm, the phenomenon is very obvious. The study paves a new way for the blood quick check and diagnosis of diseases.

To assess the feasibility, safety and effectiveness of video-assisted thoracoscopic sympathectomy (VATS) for the treatment of congenital long QT syndrome.Under general anaesthesia, pleural cavity was entered via two or three small incisions in the left intercostal space. The left thoracic sympathetic chain was identified and resected from T2 approximately T5. The lower one at the third of the left stellate ganglion was also resected.VATS resulted in a significant shortening in corrected QT intervals in three patients. The average QT interval of the four patients was 537.5 ms before VATS and 512.5 ms after VATS. The heart rate of the patients remained unchanged. There were no major peri-operative complications apart from mild ptosis of the left upper eyelid in one patient who recovered in the following days. The syndrome recurred in one patient in syncopal events in four months after VATS.VATS is a safe as well as an effective technique for the treatment of congenital long QT syndromes.

The technology of infrared absorption spectrum is a branch of optical ment measurement technology, and the research on the application of infrared spectrum plays an important role in the development of technology of optical measurement. In this paper, the analysis technology of blood infrared absorption spectrum is presented. By comparison, the difference of the spectra between normal and abnormal blood samples was obtained. The infrared absorption spectra of normal blood sample and abnormal blood sample were detected, and the differences between the spectra are presented. And the analysis results of the infrared absorption spectra of normal whole blood, serum and hyperglycemia are presented also. All of these provide an experimental basis for the diagnosis of diseases, which is valuable for application. This technology features easy operation, convenient analysis and suitability for advanced experiment. The work offers a new way in the research on the application of infrared absorption spectrum.

To investigate the effect of beta-blockers on spatial dispersion of ventricular repolarization following acute myocardial ischemia.Twenty sheep were randomized into control (normal saline,iv) and atenolol (1.5 mg/kg,iv) group. Acute myocardial ischemia was induced by occlusion of the obtuse marginal coronary artery. Unipolar ECG was simultaneously acquired from 64 epicardial sites in both ischemic and non-ischemic regions. Activation-recovery intervals (ARI), an index of ventricular repolarization, was determined from the epicardial ECG. The difference between the longest and shortest ARI was defined as ARI dispersion.Ischemic zone in atenolol group was less than that of control group (13 %+/-2 % vs 19 %+/-3 %, P=0.04). In the control group, pooled ARI dispersion was increased by (18+/-21), (27+/-21), and (16+/-10) ms at 30, 60, and 90 min of coronary artery occlusion respectively (P0.01), whereas in the atenolol group, ARI dispersion was only increased by (6+/-4), (6+/-7), and (2+/-7) ms respectively (P0.05).These findings indicate that atenolol suppresses ischemia-induced increase in spatial dispersion of ventricular repolarization, which may explain the antiarrhythmic effect of beta-blockers.