1. Glycosyltransferase GLT8D2 Positively Regulates ApoB100 Protein Expression in Hepatocytes
- Author
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Hong-Shan Wei, Fei Zhao, Yu-Tao Zhan, Hong-Lian Wei, and Le-Ping Zhong
- Subjects
Very low-density lipoprotein ,Glycosylation ,Apolipoprotein B ,Lipoproteins, VLDL ,glycosyltransferase ,Catalysis ,Gene Expression Regulation, Enzymologic ,Article ,apoB100 ,lcsh:Chemistry ,Inorganic Chemistry ,chemistry.chemical_compound ,GLT8D2 ,Glycosyltransferase ,Humans ,Secretion ,Physical and Theoretical Chemistry ,Cloning, Molecular ,lcsh:QH301-705.5 ,Molecular Biology ,Spectroscopy ,Triglycerides ,non-alcoholic fatty liver disease ,chemistry.chemical_classification ,Regulation of gene expression ,biology ,Organic Chemistry ,Glycosyltransferases ,General Medicine ,Hep G2 Cells ,In vitro ,Computer Science Applications ,Fatty Liver ,lcsh:Biology (General) ,lcsh:QD1-999 ,chemistry ,Biochemistry ,Apolipoprotein B-100 ,biology.protein ,Hepatocytes ,lipids (amino acids, peptides, and proteins) ,Glycoprotein - Abstract
Non-alcoholic fatty liver disease (NAFLD) is characterized by triglyceride (TG) accumulation in hepatocytes. Very low density lipoprotein (VLDL) is a major secretory product of the liver that transports endogenously synthesized TG. Disrupted VLDL secretion may contribute to the accumulation of TG in hepatocytes. ApoB100 (apolipoprotein B100) is a glycoprotein and an essential protein component of VLDL. Its glycosylation may affect VLDL assembly and secretion. However, which glycosyltransferase catalyzes apoB100 glycosylation is unknown. In this study, we cloned the GLT8D2 (glycosyltransferase 8 domain containing 2) gene from HepG2 cells and generated a series of plasmids for in vitro studies of its molecular functions. We discovered that GLT8D2 was localized in the ER, interacted with apoB100, and positively regulated the levels of apoB100 protein in HepG2 cells. Based on these results, we propose that GLT8D2 is a glycosyltransferase of apoB100 that regulates apoB100 levels in hepatocytes.
- Published
- 2013
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