17 results on '"Le Clainche I"'
Search Results
2. TOWARD THE DECIPHERING OF CHROMOSOME STRUCTURE IN VITIS VINIFERA
- Author
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Canaguier, A., primary, Le Clainche, I., additional, Bérard, A., additional, Chauveau, A., additional, Vernerey, M.S., additional, Guichard, C., additional, Le Paslier, M.C., additional, Di Gaspero, G., additional, Coriton, O., additional, Brunel, D., additional, and Adam-Blondon, A.-F., additional
- Published
- 2014
- Full Text
- View/download PDF
3. PHYSICAL ANCHORING AND INTEGRATED GENETIC MAPPING AMONG FIVE ELITE CULTIVARS OF VITIS VINIFERA L.
- Author
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Vezzulli, S., primary, Troggio, M., additional, Coppola, G., additional, Jermakow, A., additional, Malacarne, G., additional, Facci, M., additional, Carthwright, D., additional, Doligez, A., additional, Moroldo, M., additional, Pindo, M., additional, Stefanini, M., additional, Scalabrin, S., additional, Le Clainche, I., additional, Paillard, S., additional, Grando, M.S., additional, Adam-Blondon, A.-F., additional, Thomas, M.R., additional, Morgante, M., additional, This, P., additional, and Velasco, R., additional
- Published
- 2009
- Full Text
- View/download PDF
4. Improved PCR-Walking for Large-Scale Isolation of Plant T-DNA Borders
- Author
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Balzergue, S., primary, Dubreucq, B., additional, Chauvin, S., additional, Le-Clainche, I., additional, Le Boulaire, F., additional, de Rose, R., additional, Samson, F., additional, Biaudet, V., additional, Lecharny, A., additional, Cruaud, C., additional, Weissenbach, J., additional, Caboche, M., additional, and Lepiniec, L., additional
- Published
- 2001
- Full Text
- View/download PDF
5. Physical Anchoring and Integrated Genetic Mapping among Five Elite Cultivars of Vitis vinifera L
- Author
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Vezzulli, S., Troggio, M., Coppola, G., Jermakow, A., Giulia Malacarne, Facci, M., Carthwright, D., Doligez, A., Moroldo, M., Pindo, M., Stefanini, M., Scalabrin, S., Le Clainche, I., Paillard, S., Grando, M. S., Adam-Blondon, A-F, Thomas, M. R., Morgante, M., This, P., Velasco, R., Peterlunger, E., Digaspero, G., and Cipriani, G.
6. A physical map of the heterozygous grapevine 'Cabernet Sauvignon' allows mapping candidate genes for disease resistance
- Author
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Scalabrin Simone, Le Clainche Isabelle, Brunaud Veronique, Guichard Cecile, De Berardinis Veronique, Cruaud Corinne, Canaguier Aurelie, Fabrice Legeai, Marconi Raffaella, Paillard Sophie, Moroldo Marco, Testolin Raffaele, Di Gaspero Gabriele, Morgante Michele, and Adam-Blondon Anne-Francoise
- Subjects
Botany ,QK1-989 - Abstract
Abstract Background Whole-genome physical maps facilitate genome sequencing, sequence assembly, mapping of candidate genes, and the design of targeted genetic markers. An automated protocol was used to construct a Vitis vinifera 'Cabernet Sauvignon' physical map. The quality of the result was addressed with regard to the effect of high heterozygosity on the accuracy of contig assembly. Its usefulness for the genome-wide mapping of genes for disease resistance, which is an important trait for grapevine, was then assessed. Results The physical map included 29,727 BAC clones assembled into 1,770 contigs, spanning 715,684 kbp, and corresponding to 1.5-fold the genome size. Map inflation was due to high heterozygosity, which caused either the separation of allelic BACs in two different contigs, or local mis-assembly in contigs containing BACs from the two haplotypes. Genetic markers anchored 395 contigs or 255,476 kbp to chromosomes. The fully automated assembly and anchorage procedures were validated by BAC-by-BAC blast of the end sequences against the grape genome sequence, unveiling 7.3% of chimerical contigs. The distribution across the physical map of candidate genes for non-host and host resistance, and for defence signalling pathways was then studied. NBS-LRR and RLK genes for host resistance were found in 424 contigs, 133 of them (32%) were assigned to chromosomes, on which they are mostly organised in clusters. Non-host and defence signalling genes were found in 99 contigs dispersed without a discernable pattern across the genome. Conclusion Despite some limitations that interfere with the correct assembly of heterozygous clones into contigs, the 'Cabernet Sauvignon' physical map is a useful and reliable intermediary step between a genetic map and the genome sequence. This tool was successfully exploited for a quick mapping of complex families of genes, and it strengthened previous clues of co-localisation of major NBS-LRR clusters and disease resistance loci in grapevine.
- Published
- 2008
- Full Text
- View/download PDF
7. Thirteen New Plastid Genomes from Mixotrophic and Autotrophic Species Provide Insights into Heterotrophy Evolution in Neottieae Orchids.
- Author
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Lallemand F, Logacheva M, Le Clainche I, Bérard A, Zheleznaia E, May M, Jakalski M, Delannoy É, Le Paslier MC, and Selosse MA
- Subjects
- Autotrophic Processes, Biological Evolution, DNA, Plant genetics, Heterotrophic Processes, Orchidaceae classification, Orchidaceae microbiology, Phylogeny, Symbiosis, Genome, Plastid, Orchidaceae cytology, Orchidaceae genetics
- Abstract
Mixotrophic species use both organic and mineral carbon sources. Some mixotrophic plants combine photosynthesis and a nutrition called mycoheterotrophy, where carbon is obtained from fungi forming mycorrhizal symbiosis with their roots. These species can lose photosynthetic abilities and evolve full mycoheterotrophy. Besides morphological changes, the latter transition is associated with a deep alteration of the plastid genome. Photosynthesis-related genes are lost first, followed by housekeeping genes, eventually resulting in a highly reduced genome. Whether relaxation of selective constraints already occurs for the plastid genome of mixotrophic species, which remain photosynthetic, is unclear. This is partly due to the difficulty of comparing plastid genomes of autotrophic, mixotrophic, and mycoheterotrophic species in a narrow phylogenetic framework. We address this question in the orchid tribe Neottieae, where this large assortment of nutrition types occurs. We sequenced 13 new plastid genomes, including 9 mixotrophic species and covering all 6 Neottieae genera. We investigated selective pressure on plastid genes in each nutrition type and conducted a phylogenetic inference of the group. Surprisingly, photosynthesis-related genes did not experience selection relaxation in mixotrophic species compared with autotrophic relatives. Conversely, we observed evidence for selection intensification for some plastid genes. Photosynthesis is thus still under purifying selection, maybe because of its role in fruit formation and thus reproductive success. Phylogenetic analysis resolved most relationships, but short branches at the base of the tree suggest an evolutionary radiation at the beginning of Neottieae history, which, we hypothesize, may be linked to mixotrophy emergence., (© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.)
- Published
- 2019
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- View/download PDF
8. Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei.
- Author
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Dinh Thi VH, Coriton O, Le Clainche I, Arnaud D, Gordon SP, Linc G, Catalan P, Hasterok R, Vogel JP, Jahier J, and Chalhoub B
- Subjects
- Brachypodium classification, Chromosomes, Plant drug effects, Chromosomes, Plant genetics, Colchicine pharmacology, Diploidy, Genetic Engineering methods, Genetic Variation, Models, Genetic, Phenotype, Reproducibility of Results, Species Specificity, Tubulin Modulators pharmacology, Brachypodium genetics, Genome, Plant genetics, Plant Breeding methods, Tetraploidy
- Abstract
Brachypodium hybridum (2n = 30) is a natural allopolyploid with highly divergent sub-genomes derived from two extant diploid species, B. distachyon (2n = 10) and B. stacei (2n = 20) that differ in chromosome evolution and number. We created synthetic B. hybridum allotetraploids by hybridizing various lines of B. distachyon and B. stacei. The initial amphihaploid F1 interspecific hybrids were obtained at low frequencies when B. distachyon was used as the maternal parent (0.15% or 0.245% depending on the line used) and were sterile. No hybrids were obtained from reciprocal crosses or when autotetraploids of the parental species were crossed. Colchicine treatment was used to double the genome of the F1 amphihaploid lines leading to allotetraploids. The genome-doubled F1 plants produced a few S1 (first selfed generation) seeds after self-pollination. S1 plants from one parental combination (Bd3-1×Bsta5) were fertile and gave rise to further generations whereas those of another parental combination (Bd21×ABR114) were sterile, illustrating the importance of the parental lineages crossed. The synthetic allotetraploids were stable and resembled the natural B. hybridum at the phenotypic, cytogenetic and genomic levels. The successful creation of synthetic B. hybridum offers the possibility to study changes in genome structure and regulation at the earliest stages of allopolyploid formation in comparison with the parental species and natural B. hybridum., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2016
- Full Text
- View/download PDF
9. Decoding the oak genome: public release of sequence data, assembly, annotation and publication strategies.
- Author
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Plomion C, Aury JM, Amselem J, Alaeitabar T, Barbe V, Belser C, Bergès H, Bodénès C, Boudet N, Boury C, Canaguier A, Couloux A, Da Silva C, Duplessis S, Ehrenmann F, Estrada-Mairey B, Fouteau S, Francillonne N, Gaspin C, Guichard C, Klopp C, Labadie K, Lalanne C, Le Clainche I, Leplé JC, Le Provost G, Leroy T, Lesur I, Martin F, Mercier J, Michotey C, Murat F, Salin F, Steinbach D, Faivre-Rampant P, Wincker P, Salse J, Quesneville H, and Kremer A
- Subjects
- Models, Genetic, Molecular Sequence Annotation, Phylogeny, Quercus classification, Sequence Analysis, DNA, Genome, Plant, Quercus genetics
- Abstract
The 1.5 Gbp/2C genome of pedunculate oak (Quercus robur) has been sequenced. A strategy was established for dealing with the challenges imposed by the sequencing of such a large, complex and highly heterozygous genome by a whole-genome shotgun (WGS) approach, without the use of costly and time-consuming methods, such as fosmid or BAC clone-based hierarchical sequencing methods. The sequencing strategy combined short and long reads. Over 49 million reads provided by Roche 454 GS-FLX technology were assembled into contigs and combined with shorter Illumina sequence reads from paired-end and mate-pair libraries of different insert sizes, to build scaffolds. Errors were corrected and gaps filled with Illumina paired-end reads and contaminants detected, resulting in a total of 17,910 scaffolds (>2 kb) corresponding to 1.34 Gb. Fifty per cent of the assembly was accounted for by 1468 scaffolds (N50 of 260 kb). Initial comparison with the phylogenetically related Prunus persica gene model indicated that genes for 84.6% of the proteins present in peach (mean protein coverage of 90.5%) were present in our assembly. The second and third steps in this project are genome annotation and the assignment of scaffolds to the oak genetic linkage map. In accordance with the Bermuda and Fort Lauderdale agreements and the more recent Toronto Statement, the oak genome data have been released into public sequence repositories in advance of publication. In this presubmission paper, the oak genome consortium describes its principal lines of work and future directions for analyses of the nature, function and evolution of the oak genome., (© 2015 John Wiley & Sons Ltd.)
- Published
- 2016
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10. Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome.
- Author
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Chalhoub B, Denoeud F, Liu S, Parkin IA, Tang H, Wang X, Chiquet J, Belcram H, Tong C, Samans B, Corréa M, Da Silva C, Just J, Falentin C, Koh CS, Le Clainche I, Bernard M, Bento P, Noel B, Labadie K, Alberti A, Charles M, Arnaud D, Guo H, Daviaud C, Alamery S, Jabbari K, Zhao M, Edger PP, Chelaifa H, Tack D, Lassalle G, Mestiri I, Schnel N, Le Paslier MC, Fan G, Renault V, Bayer PE, Golicz AA, Manoli S, Lee TH, Thi VH, Chalabi S, Hu Q, Fan C, Tollenaere R, Lu Y, Battail C, Shen J, Sidebottom CH, Wang X, Canaguier A, Chauveau A, Bérard A, Deniot G, Guan M, Liu Z, Sun F, Lim YP, Lyons E, Town CD, Bancroft I, Wang X, Meng J, Ma J, Pires JC, King GJ, Brunel D, Delourme R, Renard M, Aury JM, Adams KL, Batley J, Snowdon RJ, Tost J, Edwards D, Zhou Y, Hua W, Sharpe AG, Paterson AH, Guan C, and Wincker P
- Subjects
- Brassica napus cytology, Brassica napus genetics, Chromosome Duplication, Evolution, Molecular, Genome, Plant, Polyploidy, Seeds genetics
- Abstract
Oilseed rape (Brassica napus L.) was formed ~7500 years ago by hybridization between B. rapa and B. oleracea, followed by chromosome doubling, a process known as allopolyploidy. Together with more ancient polyploidizations, this conferred an aggregate 72× genome multiplication since the origin of angiosperms and high gene content. We examined the B. napus genome and the consequences of its recent duplication. The constituent An and Cn subgenomes are engaged in subtle structural, functional, and epigenetic cross-talk, with abundant homeologous exchanges. Incipient gene loss and expression divergence have begun. Selection in B. napus oilseed types has accelerated the loss of glucosinolate genes, while preserving expansion of oil biosynthesis genes. These processes provide insights into allopolyploid evolution and its relationship with crop domestication and improvement., (Copyright © 2014, American Association for the Advancement of Science.)
- Published
- 2014
- Full Text
- View/download PDF
11. A physical map of the heterozygous grapevine 'Cabernet Sauvignon' allows mapping candidate genes for disease resistance.
- Author
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Moroldo M, Paillard S, Marconi R, Fabrice L, Canaguier A, Cruaud C, De Berardinis V, Guichard C, Brunaud V, Le Clainche I, Scalabrin S, Testolin R, Di Gaspero G, Morgante M, and Adam-Blondon AF
- Subjects
- Chromosomes, Plant genetics, Genes, Plant genetics, Heterozygote, Immunity, Innate genetics, Polymerase Chain Reaction, Sequence Analysis, DNA, Signal Transduction genetics, Genome, Plant, Physical Chromosome Mapping methods, Plant Diseases genetics, Vitis genetics
- Abstract
Background: Whole-genome physical maps facilitate genome sequencing, sequence assembly, mapping of candidate genes, and the design of targeted genetic markers. An automated protocol was used to construct a Vitis vinifera 'Cabernet Sauvignon' physical map. The quality of the result was addressed with regard to the effect of high heterozygosity on the accuracy of contig assembly. Its usefulness for the genome-wide mapping of genes for disease resistance, which is an important trait for grapevine, was then assessed., Results: The physical map included 29,727 BAC clones assembled into 1,770 contigs, spanning 715,684 kbp, and corresponding to 1.5-fold the genome size. Map inflation was due to high heterozygosity, which caused either the separation of allelic BACs in two different contigs, or local mis-assembly in contigs containing BACs from the two haplotypes. Genetic markers anchored 395 contigs or 255,476 kbp to chromosomes. The fully automated assembly and anchorage procedures were validated by BAC-by-BAC blast of the end sequences against the grape genome sequence, unveiling 7.3% of chimerical contigs. The distribution across the physical map of candidate genes for non-host and host resistance, and for defence signalling pathways was then studied. NBS-LRR and RLK genes for host resistance were found in 424 contigs, 133 of them (32%) were assigned to chromosomes, on which they are mostly organised in clusters. Non-host and defence signalling genes were found in 99 contigs dispersed without a discernable pattern across the genome., Conclusion: Despite some limitations that interfere with the correct assembly of heterozygous clones into contigs, the 'Cabernet Sauvignon' physical map is a useful and reliable intermediary step between a genetic map and the genome sequence. This tool was successfully exploited for a quick mapping of complex families of genes, and it strengthened previous clues of co-localisation of major NBS-LRR clusters and disease resistance loci in grapevine.
- Published
- 2008
- Full Text
- View/download PDF
12. The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla.
- Author
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Jaillon O, Aury JM, Noel B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C, Vezzi A, Legeai F, Hugueney P, Dasilva C, Horner D, Mica E, Jublot D, Poulain J, Bruyère C, Billault A, Segurens B, Gouyvenoux M, Ugarte E, Cattonaro F, Anthouard V, Vico V, Del Fabbro C, Alaux M, Di Gaspero G, Dumas V, Felice N, Paillard S, Juman I, Moroldo M, Scalabrin S, Canaguier A, Le Clainche I, Malacrida G, Durand E, Pesole G, Laucou V, Chatelet P, Merdinoglu D, Delledonne M, Pezzotti M, Lecharny A, Scarpelli C, Artiguenave F, Pè ME, Valle G, Morgante M, Caboche M, Adam-Blondon AF, Weissenbach J, Quétier F, and Wincker P
- Subjects
- Arabidopsis genetics, DNA, Intergenic genetics, Exons genetics, Genes, Plant genetics, Introns genetics, Karyotyping, MicroRNAs genetics, Molecular Sequence Data, Oryza genetics, Populus genetics, RNA, Plant genetics, RNA, Transfer genetics, Sequence Analysis, DNA, Evolution, Molecular, Genome, Plant genetics, Polyploidy, Vitis classification, Vitis genetics
- Abstract
The analysis of the first plant genomes provided unexpected evidence for genome duplication events in species that had previously been considered as true diploids on the basis of their genetics. These polyploidization events may have had important consequences in plant evolution, in particular for species radiation and adaptation and for the modulation of functional capacities. Here we report a high-quality draft of the genome sequence of grapevine (Vitis vinifera) obtained from a highly homozygous genotype. The draft sequence of the grapevine genome is the fourth one produced so far for flowering plants, the second for a woody species and the first for a fruit crop (cultivated for both fruit and beverage). Grapevine was selected because of its important place in the cultural heritage of humanity beginning during the Neolithic period. Several large expansions of gene families with roles in aromatic features are observed. The grapevine genome has not undergone recent genome duplication, thus enabling the discovery of ancestral traits and features of the genetic organization of flowering plants. This analysis reveals the contribution of three ancestral genomes to the grapevine haploid content. This ancestral arrangement is common to many dicotyledonous plants but is absent from the genome of rice, which is a monocotyledon. Furthermore, we explain the chronology of previously described whole-genome duplication events in the evolution of flowering plants.
- Published
- 2007
- Full Text
- View/download PDF
13. Anchoring of a large set of markers onto a BAC library for the development of a draft physical map of the grapevine genome.
- Author
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Lamoureux D, Bernole A, Le Clainche I, Tual S, Thareau V, Paillard S, Legeai F, Dossat C, Wincker P, Oswald M, Merdinoglu D, Vignault C, Delrot S, Caboche M, Chalhoub B, and Adam-Blondon AF
- Subjects
- Polymerase Chain Reaction, Chromosomes, Artificial, Bacterial, Genetic Markers, Genome, Plant, Vitis genetics
- Abstract
Five hundred and six EST-derived markers, 313 SSR markers and 26 BAC end-derived or SCAR markers were anchored by PCR on a subset of a Cabernet Sauvignon BAC library representing six genome equivalents pooled in three dimensions. In parallel, the 12,351 EST clusters of the grapevine UniGene set (build #11) from NCBI were used to design 12,125 primers pairs and perform electronic PCR on 67,543 nonredundant BAC-end sequences. This in silico experiment yielded 1,140 positive results concerning 638 different markers, among which 602 had not been already anchored by PCR. The data obtained will provide an easier access to the regulatory sequences surrounding important genes (represented by ESTs). In total, 1,731 islands of BAC clones (set of overlapping BAC clones containing at least one common marker) were obtained and 226 of them contained at least one genetically mapped anchor. These assigned islands are very useful because they will link the genetic map and the future fingerprint-based physical map and because they allowed us to indirectly place 93 ESTs on the genetic map. The islands containing two or more mapped SSR markers were also used to assess the quality of the integrated genetic map of the grapevine genome.
- Published
- 2006
- Full Text
- View/download PDF
14. Improved full-length cDNA production based on RNA tagging by T4 DNA ligase.
- Author
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Clepet C, Le Clainche I, and Caboche M
- Subjects
- Arabidopsis genetics, Base Sequence, DNA, Complementary analysis, Molecular Sequence Data, RNA, Messenger genetics, RNA, Plant genetics, RNA, Plant metabolism, Reproducibility of Results, Transcription Initiation Site, Cloning, Molecular methods, DNA Ligases metabolism, DNA, Complementary biosynthesis, DNA, Complementary genetics, Gene Library, RNA, Messenger metabolism
- Abstract
Second-strand cDNA priming is a central problem for full-length characterization of transcripts. A new strategy using bacteriophage T4 DNA ligase and partially degenerate adapters is proposed for grafting a sequence tag to the end of polyribonucleotides. Based on this RNA tagging system and previously described protocols, a new method for full-length cDNA production has been implemented. Validation of the method is shown in Arabidopsis thaliana by the construction of a full-length cDNA library and the analysis of 154 clones and by 5'-RACE-PCR run on a documented experimental system.
- Published
- 2004
- Full Text
- View/download PDF
15. Distribution and phylogeny of Wolbachia inducing thelytoky in Rhoditini and 'Aylacini' (Hymenoptera: Cynipidae).
- Author
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Plantard O, Rasplus JY, Mondor G, Le Clainche I, and Solignac M
- Subjects
- Animals, Base Sequence, DNA, Complementary, Molecular Sequence Data, Phylogeny, Rickettsia genetics, Rickettsia classification, Wasps microbiology
- Abstract
Wolbachia are endosymbiotic bacteria responsible for thelytoky in several parasitoid hymenopteran genera. After finding these micro-organisms in some populations of Diplolepis spinosissimae (Hymenoptera: Cynipidae) where they are responsible for thelytoky through gamete duplication, we searched for Wolbachia spp. using specific PCR primers in nineteen other species of the Rhoditini tribe (rose gallwasps) and eight species of the 'Aylacini' tribe (gallwasps associated with herbaceous plants). Wolbachia were found in twelve Rhoditini species and four 'Aylacini' species. The most infected species have very few males (spanandry) and the thelytoky of infected species/arrhenotoky of uninfected species is confirmed by previous research based on the sex of the offspring of virgin females. Phylogenetic analyses based on the partial Wolbachia ftsZ gene sequences indicate that some strains associated with closely related gallwasps are phylogenetically distant, suggesting that cynipids have been affected by several infection events. In contrast, the five infected European species of Diplolepis harbour the same strain of Wolbachia.
- Published
- 1999
- Full Text
- View/download PDF
16. Molecular phylogeny of the Ceratosolen species pollinating Ficus of the subgenus Sycomorus sensu stricto: biogeographical history and origins of the species-specificity breakdown cases.
- Author
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Kerdelhue C, Le Clainche I, and Rasplus JY
- Subjects
- Animals, Cytochrome b Group genetics, Evolution, Molecular, Female, Geography, Hymenoptera chemistry, Hymenoptera classification, Models, Biological, Molecular Sequence Data, Pollen, Rosales parasitology, Species Specificity, Symbiosis, Trees parasitology, Hymenoptera genetics, Phylogeny, Rosales classification, Trees classification
- Abstract
The 14 species of Ficus of the subgenus Sycomorus (Moraceae) are invariably pollinated by Ceratosolen species (Hym. Chalcidoidea), which in turn reproduce in the fig florets. They are distributed mostly in continental Africa, Madagascar, and the Mascarene and Comoro Islands, but 1 species extends its geographical range all over the Oriental region. Fig-pollinator relationships are usually strictly species specific, but exceptions to the 'one-to-one' rule occur within the group we studied. In order to understand both the biogeographical history of the Ceratosolen species associated with Ficus of the subgenus Sycomorus and the origins of the specificity breakdown cases, we have used cytochrome b sequences to reconstruct a phylogeny of the fig wasps. The results show that the pollinators from the Malagasy region and those from continental Africa form two distinct clades, which probably diverged after the crossing of the Mozambique Channel by an ancestral population. The Oriental wasp species show strong affinities with the African species. The two species-specificity exceptions are due to different evolutionary events. The occurrence of the two West African pollinators associated with F. sur can be explained by successive speciation events of the mutualistic partner without plant radiation. In contrast, we hypothesize that C. galili shifted by horizontal transfer from an unknown, presumably extinct, Ficus species to F. sycomorus after this native Malagasy fig species colonized Africa., (Copyright 1999 Academic Press.)
- Published
- 1999
- Full Text
- View/download PDF
17. Molecular phylogeny of fig wasps Agaonidae are not monophyletic.
- Author
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Rasplus JY, Kerdelhué C, Le Clainche I, and Mondor G
- Subjects
- Animals, Likelihood Functions, Molecular Sequence Data, RNA, Ribosomal, 28S genetics, Sequence Analysis, RNA, Evolution, Molecular, Phylogeny, Wasps genetics
- Abstract
According to the present classification, the family Agaonidae contains all fig pollinators as well as five subfamilies of non-pollinating fig wasps. The molecular phylogeny of the family was reconstructed using partial sequences of the 28S rRNA (D1 and D2 domains). Our results show that the family Agaonidae is not monophyletic. As a consequence, we restrict the famiy to the pollinator clade, and assign the non-pollinating subfamilies to various chalcid families. Sycoecinae, Otitesellinae and Sycoryctinae are included in Pteromalidae, whereas Sycophaginae and Epichrysomallinae are left unclassified and will require more in-depth morphological studies. Moreover, we proved that the fig pollination syndrome evolved only once, early in group history. The resource due to the fig-pollinator mutualism has secondarily been colonized independently by different Chalcid lineages.
- Published
- 1998
- Full Text
- View/download PDF
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