1. Chapter 11 Release of ATP and UTP from astrocytoma cells
- Author
-
Lazarowski Er and Harden Tk
- Subjects
chemistry.chemical_classification ,P2Y receptor ,Astrocytoma ,Stimulation ,Metabolism ,Biology ,medicine.disease ,Cell biology ,Paracrine signalling ,chemistry ,Biochemistry ,medicine ,Nucleotide ,Autocrine signalling ,Receptor - Abstract
Publisher Summary The characterization of the pharmacological selectivity of the five cloned mammalian P2Y receptors (that is, the P2Y1, P2Y2, P2Y4, P2Y6, and P2Y11, receptors) has required that the metabolism and interconversion of nucleotides by the ectoenzymes expressed by 1321N1 cells should be addressed. The studies of P2Y receptors expressed in 1321N1 cells, discussed in the chapter, have led to the discovery that mild mechanical stimulation of these cells causes non-lytic release of both ATP and UTP. As such, these cells provide an excellent model for the study of the autocrine/paracrine roles that nucleotides may play. They may also prove invaluable in determining the mechanism, whereby release of nucleotides occurs from non-excitable cells. The chapter discusses the results that describe nucleotide release from 1321N1 cells. Results obtained with P2Y receptor-expressing 1321N1 cells (which were identical to those observed with control cells) are also described in the chapter.
- Published
- 1999