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281 results on '"Laurincik J"'

1. 3D-organization and spatial localization of chromatin and epigenetic marks in relation to nucleolar activity in porcine oocytes

Author

Fenner, Merle Friederike, Benc, M., Rosenbaum Bartkova , A., Pihl, Maria, Chebrout, M., Freude, Kristine, Strejeck, F., Hyttel, Poul, Lucas-Hahn, A., Laurincik, J., Bonnet-Garnier, A, Fenner, Merle Friederike, Benc, M., Rosenbaum Bartkova , A., Pihl, Maria, Chebrout, M., Freude, Kristine, Strejeck, F., Hyttel, Poul, Lucas-Hahn, A., Laurincik, J., and Bonnet-Garnier, A

Published
2023

16. The role of RNA polymerase I transcription and embryonic genome activation in nucleolar development in bovine preimplantation embryos

Author

Østrup, Olga, Strejcek, F., Petrovicova, I., Avery, Birthe Margit, Pedersen, Hanne Gervi, Lucas-Hahn, A., Niemann, H., Laurincik, J., Maddox-Hyttel, Poul, Østrup, Olga, Strejcek, F., Petrovicova, I., Avery, Birthe Margit, Pedersen, Hanne Gervi, Lucas-Hahn, A., Niemann, H., Laurincik, J., and Maddox-Hyttel, Poul

Abstract

Udgivelsesdato: JUL, The aim of the present study was to investigate the role of RNA polymerase I (RPI) transcription in nucleolar development during major transcriptional activation (MTA) in cattle. Late eight-cell embryos were cultured in the absence (control group) or presence of actinomycin D (AD) (RPI inhibition, Ad 0.2 µg/ml; total transcriptional inhibition, AD 2.0 µg/ml). Late four-cell embryos were cultured to late eight-cell stage in 0.2 µg/ml AD (MTA prevention, ADLT (long-term total transcriptional inhibition group). Embryos were processed for autoradiography, transmission electron microscopy, fluorescent in situ hybridization (ribosomal RNA, rRNA), silver staining (nucleolar proteins), and immunofluorescence (RPI). Control embryos displayed extranucleolar and nucleolar transcription, functional nucleoli, and distinct RPI localization. Nuclei (97%) showed large rRNA clusters, in 94.1% co-localized with nucleolar proteins deposits. In AD 0.2 group, only extranucleolar transcription was detected. Segregated dense-fibrillar and granular components, but no fibrillar centers, were observed. RPPI was dispersed. Nuclei (55%) presented rRNA clusters, in 38.8% co-localized with silver-stained deposits. AD 2.0 and ADLT groups displayed no transcription and disintegrating nucleolar precursors. AD 2.0 (34%) and 14% (ADLT) of nuclei presented clusters of maternally inherited rRNA. In AD 2.0 group, RPI was dispersed, but 17.2% of nuclei showed colocalization of rRNA with nucleolar proteins. In ADLT group, RPI was lacking and clustering of nucleolar proteins was hampered. In conclusion, rDNA transcription is not required for targeting of rRNA processing proteins, rRNA is maternally inherited and target to rDNA independent of transcription, and de novo transcription is required for proper nucleologenesis in cattle.

Published
2008

26. Morphological assessment of preimplantation embryo quality in cattle

Author

Maddox-Hyttel, P., Gjørret, J.O., Vajta, G., Alexopoulos, N.I., Lewis, I., Trounson, A., Viuff, D., Laurincik, J., Müller, M., Tveden-Nyborg, P., Thomsen, P.D., Maddox-Hyttel, P., Gjørret, J.O., Vajta, G., Alexopoulos, N.I., Lewis, I., Trounson, A., Viuff, D., Laurincik, J., Müller, M., Tveden-Nyborg, P., and Thomsen, P.D.

Published
2003

27. Nucleologenesis and RNA transcription reactivation in early embryos

Author

J. Pivko ... [et al.], null, Laurincik, J., Svarcova, O., Strejcek, F., Petrovicova, I., Rosenkranz, C., Niemann, H., Schellander, K., Müller, M., Mlyncek, M., Maddox-Hyttel, P., J. Pivko ... [et al.], null, Laurincik, J., Svarcova, O., Strejcek, F., Petrovicova, I., Rosenkranz, C., Niemann, H., Schellander, K., Müller, M., Mlyncek, M., and Maddox-Hyttel, P.

Published
2003

30. Nucleolar proteins and ultrastructure in bovine in vivo developed, in vitro produced, and parthenogenetic cleavage-stage embryos

Author

Laurincik, J., Schmoll, F., Mahabir, E., Schneider, H., Stojkovic, M., Zakhartchenko, V., Prelle, K., Hendrixen, P.J.M., Voss, P.L.A.M., Moeszlacher, G.G., Avery, B., Dieleman, S.J., Besenfelder, U., Müller, M., Ochs, R.L., Wolf, E., Schellander, K., Maddox-Hyttel, P., Laurincik, J., Schmoll, F., Mahabir, E., Schneider, H., Stojkovic, M., Zakhartchenko, V., Prelle, K., Hendrixen, P.J.M., Voss, P.L.A.M., Moeszlacher, G.G., Avery, B., Dieleman, S.J., Besenfelder, U., Müller, M., Ochs, R.L., Wolf, E., Schellander, K., and Maddox-Hyttel, P.

Published
2003

35. Ribosomal RNA gene expression and chromosome aberrations in bovine oocytes and preimplantation embryos

Author

Hyttel, P., Viuff, D., Fair, T., Laurincik, J., Dybdahl Thomsen, P., Callesen, H., Vos, P. L. A. M., Hendriksen, P. J. M., Dieleman, S. J., Schellander, K., Besenfelder, U., Greve, T., Hyttel, P., Viuff, D., Fair, T., Laurincik, J., Dybdahl Thomsen, P., Callesen, H., Vos, P. L. A. M., Hendriksen, P. J. M., Dieleman, S. J., Schellander, K., Besenfelder, U., and Greve, T.

Published
2001

38. Collection of bovine embryos at different tubal stages by transvaginal endoscopy

Author

Shille, V. M., Mosslacher, G., Besenfelder, U., Schmoll, F., Mahabir, E., Schneider, M., Koster, M., Hyttel, P., Laurincik, J., Brem, G., Schellander, K., Shille, V. M., Mosslacher, G., Besenfelder, U., Schmoll, F., Mahabir, E., Schneider, M., Koster, M., Hyttel, P., Laurincik, J., Brem, G., and Schellander, K.

Published
2001

39. Nucleolar proteins and ultrastructure in bovine embryos in vivo

Author

Shille, V. M., Hyttel, P., Laurincik, J., Viuff, D., Hendriksen, P. J. M., Dieleman, S. J., Besenfelder, U., Schellander, K., Shille, V. M., Hyttel, P., Laurincik, J., Viuff, D., Hendriksen, P. J. M., Dieleman, S. J., Besenfelder, U., and Schellander, K.

Published
2001

44. Risks of in-vitro production of cattle and swine embryos:aberrations in chromosome numbers, ribosomal RNA gene activation and perinatal physiology

Author

Hyttel, P., Viuff, D., Laurincik, J., Schmidt, M., Dybdahl Thomsen, P., Avery, B., Callesen, H., Rath, D., Niemann, H., Rosenkrantz, C., Schellander, K., Ochs, R. L., Greve, T., Hyttel, P., Viuff, D., Laurincik, J., Schmidt, M., Dybdahl Thomsen, P., Avery, B., Callesen, H., Rath, D., Niemann, H., Rosenkrantz, C., Schellander, K., Ochs, R. L., and Greve, T.

Published
2000

46. Activation of ribosomal RNA genes in preimplantation cattle and swine embryos

Author

Hyttel, P., Laurincik, J., Viuff, D., Fair, T., Zakhartchenko, V., Rosenkranz, C., Avery, B., Rath, D., Niemann, H., Dybdahl Thomsen, P., Shellander, K., Callesen, H., Wolf, E., Ochs, R. L., Greve, T., Hyttel, P., Laurincik, J., Viuff, D., Fair, T., Zakhartchenko, V., Rosenkranz, C., Avery, B., Rath, D., Niemann, H., Dybdahl Thomsen, P., Shellander, K., Callesen, H., Wolf, E., Ochs, R. L., and Greve, T.

Published
2000

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