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The Italian hare (Lepus corsicanus De Winton, 1898) is endemic to central and southern Italy, where it lives in sympatry with Lepus europaeus in the mainland, while in Sicily L. europaeus is absent. The only report of endoparasites in L. corsicanus in Italy dates back to 2012. After a period of protection, the population density of the Italian hare in Sicily increased. This enabled new parasitological data to be collected on 27 hares which were compared with the data collected on 15 Sicilian hares from a previous study. Trichostrongylus retortaeformis, Paranoplocephala sp. and Cittotaenia (Mosgovoyia) sp. were isolated from the intestine. T. retortaeformis was the most prevalent and abundant parasite. Its abundance was significantly higher in males than in females. No age differences emerged. T. retortaeformis abundance was not related to low body mass and was significantly higher than that calculated from the data of the previous survey. At the same time aggregation, known as a regulatory factor of host-parasite relationship, was also higher in the present study. The possible biases introduced by sampling with different methods prevent any definitive conclusions. However, the relationship between L. corsicanus and its intestinal parasite biocoenosis appears to be stable and has possibly improved, in line with the improved status of the host population.

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A 6-year-old female goose (Branta hutchinsii) from a group of ornamental exotic geese was found dead due to severe respiratory failure, followed by emission of haemorrhagic sputum and blood clots from the beak and nostrils, and then collapse. At necropsy, the cause of death was attributed to a total of 76 helminth parasites found in the trachea and lungs, then identified as Cyathostoma bronchialis. The flock was initially treated by feed with flubendazole (1200 g/1000 kg/feed) for 7 consecutive days but, at the reappearance of the respiratory symptoms 10 days after, the animals were given fenbendazole by drinking water (300 mg/L) for 7 consecutive days, but at the reappearance of the respiratory symptoms 10 days after, the animals were given fenbendazole via drinking water (300 mg/L) for 7 consecutive days. Despite these treatments, the respiratory symptoms continued to relapse 10–15 days after the end of drug administration. In the literature, there are no data regarding drugs for the treatment of C. bronchialis infestations in geese, and the use of these drugs in exotic birds occurs as “off-label” use. This case study provides information on C. bronchialis life cycle, which is still poorly studied and poorly documented today. In particular, the case provides useful suggestions for evaluating an appropriate protocol for the treatment of C. bronchialis in geese.

With the spread of anthelmintic resistance (AR), endoparasite monitoring consolidates its role for a more sustainable targeting of treatments. A survey on endoparasites in dairy goat farms of north-eastern Italy was conducted to test a monitoring approach based on a farm-tailored sample size. Farm management and parasites control practices were investigated in 20 farms through a questionnaire survey. Further, fecal samples were collected (November 2018–September 2019) from 264 animals from 13 farms and were analyzed individually with a modified McMaster method and subsequently pooled to perform a coproculture. Coccidia (78.4%), gastrointestinal strongyles (37.9%), Strongyloides (28.4%), Skrjabinema (18.9%), Trichuris (8.0%) and Nematodirus/Marshallagia (0.4%) were identified. Abundances were higher for coccidia and gastrointestinal strongyles. Haemonchus (71%) was the dominant gastrointestinal nematode. Pasture and age class resulted in the main risk factors at the multivariable analysis through a negative binomial regression model. Results from farm monitoring indicate that our approach can be a cost-effective decision tool to target treatments more effectively, but farmers need to be educated about the importance of parasitological testing, which is currently scarcely implemented, against the risk of AR.

Simple Summary In recent years, the increased presence of wildlife in habitats close to urban settlements has raised concerns about the risk of pathogen transmission from wild animals to humans due to the spread of different parasites. For this reason, a survey aimed at describing the dispersal and parasitism level of two cervid ectoparasites was carried out in the northern Apennines, in central Italy. The presence of two hippoboscids, the autochthonous Lipoptena cervi and allochthonous L. fortisetosa, native to Eastern Asia and recently recorded in Italy, were assessed on their main host species (red deer, fallow deer, and roe deer), considering host sex and age. The alien species L. fortisetosa was found to be widespread in the study area, most likely competing with L. cervi. Moreover, red deer seemed to be the favored host of both flies, with differences in sex and age class preferences. This study demonstrated the importance of regularly monitoring the populations of these parasites, especially the invasive species, due to the risks to human health, as these insects are potential vectors of pathogens. Abstract Lipoptena fortisetosa and L. cervi are hematophagous ectoparasites belonging to the Hippoboscidae family and preferentially living on cervids. In recent years, they have received specific attention due to the great increase in the abundance of their host species, and to their medical and veterinary importance as possible vectors of pathogens harmful to humans and animals. The aim of this study was to investigate the parasitism level of both of these flies on their main hosts in Italy, which are red deer, fallow deer, and roe deer, and to highlight a possible preference for a species, sex, or age class among the hosts. Deer keds were collected by examining 326 cervids hunted in the Tuscan-Emilian Apennines. Outcomes showed that L. fortisetosa has greatly spread throughout the study area, where it competes with the autochthonous L. cervi. Moreover, red deer was the favored host species of both ectoparasites, while different preferences for host sex and age classes were observed in the two hippoboscids. The regular monitoring of deer ked populations, especially the allochthonous L. fortisetosa, which is continuously spreading in Europe, is recommended to expand the knowledge on these parasitic species that are potentially dangerous to public health.

In Northern Italy, a hastening of hare population decline was noticeable from 2008. In the same year hunters reported a sudden increase of hares infected by Taenia sp. larvae, whose morphology was consistent with T. pisiformis cysticerci. The aim of the survey was: i)to identify the parasites through morphological features and molecular techniques; ii)to quantify the prevalence and abundance of cysticerci in hunted hares; iii)to describe pathological aspects of parasite-induced lesions; iv)to evaluate the short-term trend of the infection comparing two different hunting seasons; v)to highlight possible relationship between T. pisiformis infection and hare-related variables. In 2013, 2015 the viscera of 54 and 61 hares legally hunted in agro-ecosystems of the Po Plain were collected. Peritoneum, liver and lungs were examined for cysticercosis; abundance was estimated counting superficial parasites in liver; parasites were microscopically identified by shape and measure of both large and small hooks. One cysticercus from each hare was analized by a PCR targeting Taeniid species and then sequenced. Frozen liver, lungs and gastrointestinal peritoneum were macroscopically observed and, after thawing, representative samples from the available organs were collected for histologic examination to verify parasitic cysts and the subsequent damage of the involved organs. Sex, weight and age class of the animals were recorded. Generalized linear models were used for statistical analysis. T. pisiformis was isolated in 8 hares in 2013 (prevalence 14.8%; abundance range: 0–400; mean abundance 17.8) and in 2 hares in 2015 (prevalence 3.28%; abundance range: 0–180; mean abundance 3.22). Identification was confirmed morphologically and by PCR. The DNA sequencing confirmed T. pisiformis in all samples. The sequences were all identical each-other. Infection was significantly related with adult age class, sampling year and low full-weight. Epidemiological and pathological pattern suggest both a possible role on host population health and a tendency toward host-parasite equilibrium., Highlights • Hastening of hare population decline was noticeable in Northern Italy. • Hunters reported a sudden increase of hares infected by Taenia sp. larvae. • T. pisiformis was isolated. • Observed pattern suggest both a possible role on host population health and a tendency toward host-parasite equilibrium.

The regulation of parasite populations is a very interesting field of study, relevant for both farm and wild host species. Traditionally, the mortality of parasites due to parasite-induced host mortality has been considered the main feature giving rise to the regulation and stability of host–parasite interaction, enhanced by the negative binomial distribution of parasites in host populations. Nevertheless, different possible density-dependent mechanisms of parasite regulation are likely to occur. For small strongyles (Subfamily: Cyathostominae), the most widespread intestinal parasites of equids, one of the mechanisms possibly regulating parasite population is the negative feed-back of luminal adult parasites on mucosal larval stage development. This paper proposes a deterministic model consistent with empirical data collected on donkey small strongyles. Its most innovative aspect is the inclusion of mucosal hypobiotic larvae regulation induced by adult parasite abundance. The explicit calculation of the expected value of the larval development rate, given the negative binomial distribution of adult parasites, was provided. Parasite-induced host mortality was irrelevant in the experimental donkey population studied and not included in the model, while density-dependent mortality of endogenous parasite stages was included. The main aim was to evaluate the possible stabilizing effect of the regulation of larval development. For this purpose, the comparison of the full model with a simplified model in which development rate is a fixed parameter was performed. The behaviour of the models after additional cyclical mortality was also studied. Cyclical parasite mortality can be viewed as the result of regular anthelmintic control measures used in the field. The main result obtained is that parasite extinction due to additional cyclical mortality is more difficult to be obtained if mucosal larvae development is regulated, notwithstanding the small transmission rate of the full model. Ecological regulation of parasite dynamics appears as a significant obstacle to parasite control; in particular the regulatory effect of adult parasites on mucosal larvae development enhances parasite viability allowing parasites to reach high population abundance with lower transmission rate and represents a clear advantage in terms of parasite survival to perturbations.

Intestinal parasites has been traditionally regarded as a possible cause of horse colic (Love, 1992, Equine Vet. J., 24:5-9); however, the evidence of small strongyles causing intestinal disorders has never been clearly demonstrated and the evidence of parasites causing colic in horse was recently questioned (Uhlinger, 2007, Vet. Clin. North Am. Equine Pract., 23:509-517). Aim of the study was the comparison of parasitological status between subjects with or without colic syndrome, with particular regard to small strongyles infections. Quali-quantitative coprological analysis were performed on 86 horses referred to the Department of Veterinary Medical Sciences: 43 horses with colic (22 and 21 treated with medical or surgical management, respectively) and 43 horses referred for different reasons (controls). Coprocolture was performed on positive samples to differentiate small strongyles and S. vulgaris infections. Data were analysed using STATA 11.2. Strongyle egg prevalence was 39.5%, the mean number of eggs per gram of faeces (epg) was 145.34. Four horses (4.6%) had S. vulgaris infection. Negative binomial multiple regression highlighted no influence of horse sex on epg, while there was a negative relationship between age (in month) and epg; the same analysis revealed a significant difference of epg (p

Since the Seventies, a reduction of the European brown hare population (Lepus europaeus) has been observed in most European Countries, including Italy. This reduction suddenly accelerated, at least in some italian areas areas, from 2008 and is still continuing. The studied hares come from the eastern hunting-management district of the Province of Bologna (Emilia-Romagna Region) known as ATCBO2. In ATCBO2, both the hares hunted in the hunting areas and the hares captured in the protected areas severely dropped from 2008-09. For example, about six thousands hares were hunted in 2009-10 while about four thousands were hunted in 2015-16; consistently, captured hares were more than 2 thousands in 2008-09 and about seven hundred in 2015-16. However, the same data suggest a reduced speed of hare decline from 2013-14, when the hare population seems to stabilize at low densities. 54 and 61 hares legally hunted in 2013-14 and 2015-16 hunting season, respectively, were examinded for helminth parasites. Sex and full weight was recordered by hunters; age was estimated observing Stroh’s tubercle. No significant difference in the sampled hare population was found between the two hunting seasons: sex-ratio, age-ratio and full weigh was similar in both years. In both hunting seasons, the intestinal nematode Trichostrongylus retortaeformis and the visceral larval form of the tapeworm Taenia pisiformis (cysticercus) were collected; moreover, lesions ascribed to lung nematode infection were observed in 3+3 hares collected in each hunting season. In the second hunting season only, the nematode Trichuris sp. and the filarial nematode Micipsella numidica were also collected from the intestine and the sierosae, respectively. Parasite biodiversity was, therefore, particularly low in both seasons, but improved in the second one. Significant differences between the two hunting seasons were observed for both T. pisiformis and T. retortaeformis. T. pisiformis infection was less frequent in 2015-16 (3.28%) than in 2013-14 (14.81%); on the contrary, T. retortaeformis abundance was significantly higher in the second hunting season. While T. pisiformis abundance appeared to be negatively related with the weight of the hares in the first hunting season, T. retortaeformis abundance was higher in heavier hosts in both seasons. Both parasites are typical of adult hosts: T. retortaeformis was significantly more abundant in adults and T. pisiformis cysticerci were never isolated in animals younger than 7 month. From 2007-08 hunters (and mass-media) reported a sudden increase of hares infected by T.pisiformis cysticerci, seldom or even never found before in the same area. Previous reports about hare parasites showed quite composite helminth communities, with different helmith species parasitising the gastro-intestinal tract of the hares. The results of the present study reveal an atypical parasite situation, with the strong dominance of one gastro-intestinal helminth species only and the appearance of heavy infections due to cysticerci of T. pisiformis. The difference in parasite infections suggest a modification of the host-parasite relationship over time. The hares examined in the first hunting season show a very critical parasite situation, with T. pisiformis possibly affecting host condition and a strongly depauperated intestinal helminth community dominated by T. retortaeformis alone. The reduction of T. pisiformis infection two years later and the appearance of other helminth species suggest improved host-parasite relationship and could indicate that the period of hare population decline is going to end up. It is impossible to know if parasites played any role in promoting hare population decline, but it is very difficult that T. pisiformis alone could have induced such a dramatic host decline. Hosts and parasites appears, rather, to be tied by a common ecological destiny.

The aim of this retrospective study was to describe the use of simple interrupted suture pattern for the closure of the abdomen after ventral midline celiotomy in horses and to evaluate the prevalence of resulting incisional complications. Seventy horses undergoing abdominal surgery (2006–2015) and surviving at least 30 days were included in the study. Closure technique of the abdominal wall was performed on three layers. Linea alba was closed with a simple interrupted pattern using number two coated braided absorbable polyglycolic acid suture. Follow-up information was recovered at the 90th day postoperatively. Incisional complications considered included drainage, infection, dehiscence, and herniation. The complications observed in the present study using simple interrupted suture pattern were 4.29% (3 of 70), lower than reported in literature with different suture pattern. Simple interrupted suture pattern could be a valid option for the closure of linea alba after ventral midline celiotomy in horses.

Even though dairy cows are known carriers of Arcobacter species and raw or minimally processed foods are recognized as the main sources of human Arcobacter infections in industrialized countries, data on Arcobacter excretion patterns in cows and in milk are scant. This study aimed to identify potentially pathogenic Arcobacter species in a dairy herd and to investigate the routes of Arcobacter transmission among animals and the potential sources of cattle infection and milk contamination. A strategy of sampling the same 50 dairy animals, feed, water, and milk every month for a 10-month period, as well as the sampling of quarter milk, animal teats, the milking environment, and animals living on the farm (pigeons and cats), was used to evaluate, by pulsed-field gel electrophoresis (PFGE), the characteristic patterns in animals, their living environment, and the raw milk they produced. Of the 463 samples collected, 105 (22.6%) were positive for Arcobacter spp. by culture examination. All the matrices except quarter milk and pigeon gut samples were positive, with prevalences ranging from 15 to 83% depending on the sample. Only three Arcobacter species, Arcobacter cryaerophilus (54.2%), A. butzleri (34.2%), and A. skirrowii (32.3%), were detected. PFGE analysis of 370 isolates from positive samples provided strong evidence of Arcobacter circulation in the herd: cattle likely acquire the microorganisms by orofecal transmission, either by direct contact or from the environment, or both. Water appears to be a major source of animal infection. Raw milk produced by the farm and collected from a bulk tank was frequently contaminated (80%) by A. butzleri ; our PFGE findings excluded primary contamination of milk, whereas teats and milking machine surfaces could be sources of Arcobacter milk contamination.

This study aimed to set up a method for the long-term management of Adriatic sturgeon, through the analysis of sex steroids and the genetic profiling of individuals in order to maximize the already reduced genetic variability of this species. Ten A. naccarii adults (nine of captive origin, one captured in the Ticino river and then moved into captivity) and eight subadults located in a semi-natural land-locked pond in Abbiategrasso (Milan, Italy) were analysed. Plasma testosterone differed significantly in the eight analysed subadults allowing their differentiation into two groups: the first group with an average testosterone concentration of 5.42 ± 1.31 ng/ml (probably female) and the second group with an average of 423.14 ± 75.97 ng/ml (probably male), as subsequently confirmed by artificial stripping. The plasma testosterone level was also significantly different between adult males and females (371.37 ± 43.58 vs. 95.34 ± 51.10 ng/ml), while the E2 levels showed no significant differences. Animals were genotyped on the basis of 10 microsatellite loci and their parental relationships were defined: four adults, two females and two males, generated the eight subadults. On the basis of pedigree analyses and genetic distances, 15 unrelated couples were identified for the future breeding seasons. Finally, the adult female captured in the Ticino River showed an interesting genetic profile, widely different from all of the other 17 specimens analysed, and represents a valuable source of genetic diversity.

Doctor fish (Garra rufa) have recently been used for aesthetic purposes and as a medical treatment in patients with psoriasis (ichthyotherapy). For this particular kind of human therapy it is essential to guarantee adequate hygienic conditions for both people and fish. The aim of this study was to test two concentrations of water disinfectants, chloramine T and peracetic acid, on Garra rufa to ascertain possible exposure damage to the epidermis and gills. Fish were exposed to 2 mg/l and 10 mg/l of chloramine T and to 15 μl/l and 45 μl/l of peracetic acid in a 40-minute static bath up to six times a day for one week. The epidermis and gills were checked for histological changes and the number of epidermal mucous cells, club cells and taste buds were quantified; mucous cells were also characterized histochemically to detect alterations in mucin production. No mortality or severe histological changes were found in treated or control fish. Cell count showed a significant increase (p

During the past decades the interest in surveillance and monitoring of wildlife diseases has grown internationally. The main reasons could be the following: a) increased size of many wildlife populations that host pathogens affecting humans; b) the increased economic relevance of some wildlife disease; c) the role played by infections/diseases in the conservation of some wild endangered species. According to the above-described epidemiological situations there is an international need to develop appropriate strategies for the early detection, monitoring and survey of infectious diseases in wildlife. The paper reviews the epidemiological assumptions on which disease surveillance, monitoring and surveys are, or should be, based. The main conclusions are: 1) wildlife disease surveillance and monitoring are long lasting activities that should be implemented when legal bases are available; 2) a wildlife disease introduced in a free area is more likely to be detected early using passive rather than active surveillance; 3) the definition of the "suspect case" largely affects the sensitivity of the whole passive surveillance; thus the suspect case definition should be modulated according to the level of risk; 4) in both active surveillance and monitoring, sampling plays an important role. The sensitivity of any active surveillance/monitoring system is highly dependent from the sampling unit that we define as: "the host target subpopulation, whose size can maintain the pathogen during a defined inter-sampling interval". Such definition merges the ecological, epidemiological and mathematical approaches aimed in controlling or eradicating infections in both livestock and wildlife; 5) When dealing with the conservation-disease interface, a standardized risk assessment procedure including risk mitigation has to become the rule.

Italian hare Lepus corsicanus De Winton, 1898, is a true species living in simpatry with Lepus europaeus in mainland Italy and with Oryctolagus cuniculus in Sicily, where L. europaeus is absent. Up to date, nobody has studied the helminths of this endemic Italian Mammal. Therefore, in order to describe Italian hare gastro-intestinal helminths, gastro-intestinal tract of 29 Italian hares, coming from mainland Italy (#14) and from Sicily (#15) were collected between 1997 and 2009. Twentyfive hares were positive for at least one parasite (86 %). Six parasite species were isolated, 2 cestodes (Cittotaenia pectinata, prevalence 3 %) and Paranoplocephala sp., 3 %) and 4 nematodes (Trichostrongylus retortaeformis, 86 %, Graphidium strigosum, 14 %, Trichuris sp., 10 % and Teladorsagia circumcincta, 7 %). Both Teladorsagia circumcincta circumcincta and Teladorsagia circumcincta trifurcata morphotypes were identified. Comparison with available data regarding L. europaeus and O. cuniculus in Italy is provided. Being not T. circumcincta and Paranoplocephala sp. typical parasites of leporids, a description of the observed specimens is given.

Ixodes festai Rondelli, 1926 is a poorly known bird parasite tick. Its immature forms have not been described yet, while the adult forms only insufficiently, especially the male. In this note the presence of the male of Ixodes festai for the first time in Sardinia (Italy) is reported and a detailed redescription is provided. Morphometric data as well as photographs performed with one optical and one electron microscope (ESEM FEI Quanta 200) are also shown. Ixodes festai Rondelli, 1926 est une espèce mal connue, parasite des oiseaux. Les stades immatures sont toujours actuellement non décrits, et les stades adultes eux-mêmes restent encore très partiellement décrits, notamment le mâle. Dans cet article, les auteurs décrivent le mâle de l’Ixodidae Ixodes festai signalé pour la première fois en Sardaigne (Italie), et une nouvelle description détaillée est fournie. En outre, des données morphométriques et des photographies prises par un microscope optique et par un microscope électronique ESEM FEI Quanta 200 complètent cet article.

Factors affecting the thermophilic Campylobacter fecal shedding in Italian dairy farming conditions have been investigated in a 12-month longitudinal study performed in a dairy farm authorized to sell raw milk in Italy. Fifty animals were randomly selected from 140 adult and young animals, and fecal sampling was performed six times at two month intervals; additionally at each sampling, three trough water samples and two trough feed samples were collected for both adult and young animals. Samples were analyzed by real-time PCR and cultural examination. Overall 33 samples (9.7%) were positive for thermophilic Campylobacter by real-time PCR: 26 out of 280 (9.2%) fecal samples, six out of 36 water points (16.6%) and one of the 24 feed samples (4.2%). Campylobacter jejuni was isolated from 6 out of 280 samples; no other Campylobacter sp. was isolated. A higher but not significant positivity was observed in fecal samples of younger animals (11.33%% versus 6.92% of adult animals) and a higher and significant positivity was observed in water samples collected from the water troughs of young animals. A distinct temporal dynamic trend was observed during the study period for both cows and calves, with two prevalence peaks between November/December and between May/July. Several factors such as calving, housing practices, herd size, management practices forcing together a higher number of animals, variations in feeding or water source, which were previously reported as cause of temporal variation in different farming conditions, could be excluded as cause of the two seasonal peaks in this study. The factors affecting the seasonality of Campylobacter shedding in the dairy herds remain unclear and warrant further investigation. The results of the present study indicate that special attention should be paid to farm hygiene management in farms authorized to produce and sell raw milk with increased surveillance by the authorities in certain periods of the year.

The objective of this study was to analyse over time the evolution of E. coli O157:H7 faecal shedding in a dairy herd producing raw milk for direct human consumption. The study was performed between October 2012 and September 2013 in an average size Italian dairy farm where animals are housed inside the barn all over the year. The farm housed about 140 animals during the study – 70 cows and 70 calves and heifers. Twenty-six animals were randomly selected from both the cows and young animals group, and faecal sampling was performed rectally six times two months apart in each animal. Eleven animals were culled during the study and a total of 285 faecal samples were collected. At each faecal sampling, three trough water samples and two trough feed samples were also collected for a total of 36 water samples and 24 feed samples. Samples were analysed by real time polymerase chain reaction (RT-PCR) and culture. Overall, 16 (5.6%) faecal samples were positive for E. coli O157 by RT-PCR. Cultural examination found 9 (3.1%) samples positive for E. coli O157; all the isolates were positive for stx1, stx 2 and eae genes. One (4.1%) feed sample was positive for E. coli O157 by RT-PCR; none of the water samples was positive for E. coli O157. The model highlighted a general significant reduction of the number of positive samples observed during the study from the first to the sixth sampling (P=0.000) and a positive relation between the presence of positive samples and average environmental temperature (P=0.003). The results of the study showed that in an Italian dairy farm housing animals all year, faecal shedding of E. coli O157 followed the same temporal trend reported for other types of farming. The enhanced faecal shedding during warmer months may have a significant impact on environmental contamination and the safety of raw milk and its byproducts.

Pigs are increasingly used in medical research as transgenic laboratory animals; however, little knowledge is presently available concerning their welfare assessment. The aim of the present study was to investigate some welfare-related parameters of transgenic pigs intended for xenotrasplantation (human decay-accelerating factor (hDAF)) when compared with their conventional (i.e. not transgenic) close relatives (full sibs and half sibs). A total of 14 Large White female transgenic pigs and 10 female non-transgenic (conventional) pigs from four litters were used. All pigs were from the same conventional boar, donor of the semen treated for sperm-mediated gene transfer. During the experiment, BW ranged from 50 to about 80 kg and pigs were weighed at the beginning and at the end of the experiment. Animals were subjected to a set of behavioural tests: a human approach test (HAT), a novel object test (NOT) and an open-door test (ODT). Food preferences were tested through the offer of different foods (banana, apple, carrot, cracker and lemon). During a 4-day period, pigs were diurnally videotaped to study the prevalence of the different behaviours and social interactions (aggressive and non-aggressive interactions). At the end of the trial, cortisol level had been assessed on bristles. No significant differences ( P>0.05) were observed between hDAF transgenic and conventional pigs with respect to growth traits, reactivity towards unexpected situations (HAT, NOT, ODT), food preferences, main behavioural traits, social interactions and hair cortisol.

The main aim of this study was to evaluate the relationship between the social hierarchy of plain zebra, Equus quagga, and the level of parasitism. For the study 141 fecal samples from the same number of animals were collected within the two major populations of E. quagga of Uganda (Lake Mburo Conservation Area and Kidepo Valley National Park). Quantitative (eggs per gram of faeces) and qualitative parasite assessment were performed with standard methods. The relationship between parasite burden and individual host features was analyzed using Generalised Linear Models. Strongyles, cestodes, Strongyloides sp. and oxiurids where present in the examined samples. Social rank and age class significantly affect all parasites’ abundance with dominant individuals being less parasitized than subordinate individuals, regardless of the parasite groups excluding oxiurids. Sex could not been shown to be related with any of the found parasites. Age was positively related with strongyles and oxiurids abundance and negatively related with cestodes and Strongyloides sp. The main result of the present study was the evidence that social status influences parasite level with dominant zebras shedding less parasite eggs than subordinate ones. Social rank appears, therefore, as an important factor giving rise to parasite aggregation in plain zebras.

Little is known about the normal or pathologic yeast flora in healthy and critically ill neonatal foals. The aims of this study were to evaluate the yeast flora colonizing the mucous membranes of the digestive tract (oropharynx and rectum mucous membranes) of healthy and hospitalized foals and to find out risk factors involved in yeast colonization of foals referred to a neonatal intensive care unit. A total of 240 swabs were collected from 21 healthy (group A) and 39 sick (group B) foals. In 14 of the 60 foals, yeast was isolated in at least one sample (23.3%): 3 of the 21 foals (14.3%) were positive in group A and 11 of 39 foals (28.2%) were positive in group B. The yeasts were isolated from rectal swabs obtained from none in healthy foals, whereas 5 of the 39 sick foals were positive; however, this difference was not statistically significant. No significant difference was also detected regarding oropharyngeal swabs between healthy (3/21) and sick (10/39) foals. The risk factors significantly associated with the isolation of yeasts from rectal swabs were female sex, treatment with oral antibiotics, and stressful diagnostic–therapeutic procedures. The only risk factor significantly associated with the isolation of yeast from oropharyngeal swabs was the treatment with antacids and gastroprotectants. The results show that fungi present in the gastrointestinal tract of neonatal foals were mainly environmental yeasts and suggested the absence of a stable fungal colonization. Candida was the genus frequently isolated in hospitalized foals, just as it is isolated in critically ill human neonates.

The influence of host and parasite-related factors on the strongyle infection in 50 horses coming from 6 European countries and slaughtered in Italy for meat production was investigated using a multivariable modelling approach. The study was carried out by examining adult helminths, faecal eggs (identified by culture to the third larval stage) and mucosal larval stages of Cyathostominae. A modified Transmural Illumination technique (TMI) has been performed and Cyathostominae empty mucosal cysts were also evaluated in order to obtain further indications about small strongyles dynamic. All species found in this study were previously reported in European horses. Major differences were detected comparing Hungarian (#24) and Italian (#13) horses. Sex was confirmed as uninfluential, while relations with host age were only partially consistent with the development of acquired resistance. The analysis of both mucosal Cyathostominae larvae (more in Italy) and of the percentage of empty cysts (higher in Hungary) along with lower large strongyle abundance in Hungary allowed to hypothesise a wider use of anthelmintic treatments in Hungarian horses compared to Italian ones. The results regarding adult Cyathostominae (no significant differences nor regarding age or origin) suggested the important role of ecological interactions between larval and adult stages in regulating small strongyle populations.

Anticoagulant poisoning is a common rodent control method in urban areas, but rodents may exhibit versatile feeding behavioural habits in varying environmental conditions. This study has been conducted to determine those environmental factors that could directly influence bait consumption and which therefore may affect an urban rodent control plan carried out with chemical methods. In the city of Bologna (Italy), 2500 bait stations have been monitored in various urban biotopes and the bait consumption studied in relation to several environmental factors, using both bivariate and multivariate analysis. Results reveal that the rodent patch distribution triggers the avoidance of baits placed outside of their home-range, hence the importance on bait placement. The logistic regression model confirms that vegetation, water and harbourage supplies were important variables in determining bait consumption, probably because they may spatially and temporarily facilitate the agoraphobic rodent movement, ensuring undisturbed bait consumption. The role of predators (dogs and cats) and food competitors (synanthropic birds) remains insignificant. However, bait eating invertebrates may really consume part of the bait. For a proper rodent control plan the first real need is to minimize the alternative food sources, which may compete with baits. Weather conditions in which rodents could possibly pass unnoticed (fog and rainfall) may encourage bait consumption, vice versa with a cold climate. The considered environmental factors have satisfactorily explained bait consumption, highlighting the importance of human influence. The study of environmental factors may be useful in categorizing several site-specific conditions where rodent control efforts should be targeted, enhancing any intervention by matching the correct and specific strategy.

Between December 2005 and November 2006, a survey on coccidiosis of wild and captive mouflons living in the Orecchiella Natural Reserve (Tuscany, Italy) was performed on faecal samples collected approximately every two months. A total of 96 samples were collected, 55 of which from wild mouflons and 41 from captive mouflons. On these faecal samples qualitative and quantitative parasitological analyses were performed; moreover, faecal cultures were made in order to identify the involved Eimeria species. Significantly higher prevalence (%) and mean intensity (mean OPG +/- SD) of coccidian infection resulted for captive mouflons (73.17% and 814.6 +/- 1297.2 OPG) comparing to wild mouflons (36.73% and 112.7 +/- 268.7 OPG). Eimeria parva, E ovinoidalis, E. bakuensis, E. ahasata, E. intricata, E. crandallis, E. granulosa, E. faurei and an Eimeria species very similar to the descriptions previously reported for E. arloingi and E. yakimoffmatschoulsky, were isolated.

Beef cattle heads (195 heads, 6 batches) imported for fattening from France to Italy were examined. Coprological qualitative and quantitative tests were performed, and the results analysed in relation to sex, breed, age, date of arrival, geographical origin (French department in which the animal was bred), and gathering centre (French department in which the animal was grouped with the others before travelling to Italy). The following parasites were identified: Eimeriidae (overall prevalence 60.5%); Strongyles (59%); Nematodirus spp. (14.3%); Trichuris spp. (4.1%); Capillaria spp. (2.0%); Paramphistomum spp. (27.6%); Dicrocoelium spp. (3.0%); Moniezia spp. (8.7%). All the observed parasites are widely reported in beef cattle either in Italy or in France. However, the seeming absence of Fasciola hepatica was unexpected, as well as the high prevalence of Paramphistomum spp. The variables that appeared to be more linked to parasite epidemiology were sex, altitude of the geographical origin and season.

Host density is an important and widely accepted factor influencing microparasites epidemiology. In theory, host density would influence also macroparasite dynamic, although it would be achieved indirectly due to the presence of free-living infective stages of parasites. On this basis, it is expected that macroparasite abundance and prevalence would increase as host density increases, due to the higher probability for a new host to acquire infections from the environment. Nevertheless, some surveys indicate a negative relationship between host density and gastrointestinal helminth abundance in alpine chamois. On the basis of data collected from three different chamois populations, the Authors discuss the possibility that ecological factors different from host density should influence parasite biocoenosis dynamic, leading to the pattern observed in natural chamois-parasite systems.

From 1987 to 1993, 89 wolves (Canis lupus) collected throughout the whole Italian range were examined for intestinal helminth parasites. Twelve species were found, including 5 nematodes (Uncinaria stenocephala, Toxocara canis, Ancylostoma caninum, Trichuris vulpis and Toxascaris leonina) and 7 cestodes (Echinococcus granulosus, Taenia hydatigena, T. multiceps, T. pisiformis, T. ovis, Mesocestoides lineatus and Dipylidium caninum). No significant differences were detected between sexes. T. canis showed higher prevalence and numbers in youngs, while E. granulosus and T. vulpis in adults. Interference between U. stenocephala and A. caninum was detected. Parasite biocenosis was stable in respect to geographical and ecological variables.

Gli autori discutono i dati relativi all'offerta formativa riguardante il SSD VET06 nelle 15 Facoltà di Medicina Veterinaria italiane. Le informazioni raccolte hanno permesso di accertare l'estrema variabilità tra le varie sedi. Ad esempio, solo riferendosi al CdL specialistica in MV e agli insegnamenti canonici di Parassitologia e Malattie Parassitarie, hanno potuto notare notevoli differenze per tutti i parametri considerati: crediti formativi, ore per credito, ore totali e anno di attivazione. Gli autori concludono riflettendo sull'eccessiva diversificazione tra le varie sedi e sulle conseguenze che questa ha sulla preparazione degli studenti e sulla cultura parassitologica in senso lato, auspicando un confronto a livello nazionale

A three years survey on gastro-intestinal nematode egg output was carried out in four Alpine chamois herds (Rupicapra rupicapra rupicapra) from Belluno province (Italy). Data were analysed in order to detect the influence of cattle presence and chamois density on egg output, and to evaluate the stability of host-parasite relationship. As expected, variations related to the season were present, with the major peak of egg output observed in the summer. Results highlighted an unexpected inverse influence of host density on egg counts. The results are in relation to host-parasite relationship stability. Parasites seem to be able to self-regulate in the absence of parasite-induced host mortality. No effect due to cattle presence was detected.

Parasitic infections in populations of wild herbivores can affect the individual fitness and population dynamics of their hosts. In this study, the ecology of gastrointestinal (GI) parasites was investigated in an Alpine ibex colony of the Marmolada massif, eastern Alps. Both individual and environmental factors were studied to reveal their relationship with parasite prevalence and burden. In a four-year period, 414 individual faecal samples of ibex were collected on a monthly basis during summer and autumn and were subjected to quali-quantitative examinations for the identification and quantification of oocysts and eggs. Domestic ungulates grazing in the same area were also sampled to investigate the risk of GI parasite transmission. Negative binomial regression models were developed to study the influence of physiological and environmental factors on parasite burdens. The results of the qualitative examination were in agreement with the few data already present in the literature for Alpine ibex, showing high prevalence values for Coccidia and GI strongyles, low values for Cestoda and sporadic presence of whipworms. Higher burdens in kids were found for Coccidia and Cestoda. Analysis of the GI endoparasite community of cattle and sheep suggested a negligible risk of parasite transmission to the Alpine ibex population. The sanitary risk represented by Coccidia and Cestoda in ibex kids and some peculiarities of the endoparasite distribution patterns in the Marmolada ibex population call for future in-depth ecological studies to investigate their influence on the limited growth rate shown by the ibex colony during the last decade.

Veterinary students tend to disregard some disciplines in favour of those directly involved in the medical and clinical practice resulting in low course attendance and poor learning outcomes. To increase discipline’s attractiveness, an experiment of interdisciplinary teaching was developed at the Veterinary Faculty of the University of Bologna in 2016

Since the Seventies, a continual reduction of the European brown hare population (Lepus europaeus) has been observed in most European Countries, including Italy. In the Bologna Province, Emilia Romagna Region, after a recovery phase a sudden acceleration of the hare population decline was noticeable from 2008: the hare captured in protected areas for restocking dropped from about 7,000 in 2007-‘08 to 1,891 in 2014-’15. In 2013, from September 15th to October 5th, 53 hares haunted in agro-ecosystems of the Po Plain (Province of Bologna, ATC BO2) were collected for parasitological analysis. All the helminthes were collected from the stomach and the intestine using standard parasitological techniques, preserved in 70% ethyl alcohol and 5% glycerin and therefore microscopically identified following clarification with 20% lactophenol. The sex of each hare and the weight of 52 of them were recordered by hunters; age class was assigned to 51 hare out of 53 by observing Stroh’s tubercle. The animals examined were 40% adults and 60% juveniles, 57% females and 43% males. Mean weight was 3.4 Kg (range: 2.1-4.5 Kg). Only one helminth species was collected, i.e. the nematode Trichostrongylus retortaeformis. Thirty-eight out of 53 hare hosted T. retortaeformis (prevalence 72%); the mean parasite abundance was 22 helminth/examined-hare. Parasite prevalence was significantly higher in adults (95%) than in juveniles (54.8%) (Fisher exact test, p

Data about the presence of Arcobacter in wild birds are currently lacking. In this study cloacal swabs from 95 collared doves (Streptopelia decaocto), submitted to the Department of Veterinary Medical Sciences (Bologna, Italy) between 2011 and 2013 from various urban and suburban areas of the Emilia-Romagna region (Northern Italy), were tested for the presence of Arcobacter sp. by a rRNA 23S nested Polymerase Chain Reaction (PCR). Eighteen out of 95 (19%) samples showed the expected PCR product. Further cultural and molecular studies are needed to assess the Arcobacter prevalence in wild birds and elucidate their potential epidemiological role as source of animal and human infections.

The sarcoptic mange epizootic affecting chamois in the Dolomites Alps since 1995 has risen considerable concern in a management and conservation perspective, due to its strong impact on chamois and ibex populations. A remarkable amount of data has been collected by different wildlife research and management institutions, in order to analyze mange patterns and develop possible strategies to control the disease. The present study is aimed at providing a population-related figure of the spatial and temporal dynamics of clinical sarcoptic mange in alpine chamois, proposing an approach in which relevant basic concepts and parameters, as the definition of the epidemic front and its spreading speed, can be estimated and framed. The epidemic front was referred to the different mountain massifs, corresponding to well established management units of the chamois in the study area; moreover, the mange-related mortality peak at the massif level was used (in substitution of the index case/s) for temporal analysis of the disease spreading. Two speeds of the front have been estimated: a first raw average speed of about 3.38 km/year, and a second refined speed of 4.64 ± 3.12 km/year, more consistent to the variability in the field. The time series analysis showed that the impact of mange increases over the late winter months, reaching a peak in early spring.Our results strengthen the conclusions of previous studies, proposing a new frame to include other studies in progress on the alpine chamois-Sarcoptes interactions.Download the complete issue.

Aedes albopictus is a competent vector of at least 22 arboviruses, including dengue and chikungunya viruses (Gratz, 2004, Med. Vet. Entomol., 18:215-227). The potential for pathogen transmission by Ae. albopictus has increased the need to understand its ecology and population dynamics. Previous studies have shown that density-dependence can affect larval development (Lord, 1998, J. Med. Entomol., 35:825-829). Density-dependent competition for the available food resources has often been argued as the factor influencing larval development (Walsh et al., 2012, Plos One, 7:1-6). Aim of the study was to assess if larval development time is influenced by the density of laid eggs and the possible consequence of development delay on the efficacy of the larvicidal compound diflubenzuron (DFB), extensively used on a monthly basis in the national territory for tiger mosquitoes control. Ovitraps were positioned in August 2013 for the collection of Ae. albopictus eggs; eggs were counted and 48 ovitraps (24 with high density of eggs: mean 51.6, range 30-93; 24 with low density of eggs: mean 11.9; range 9-15) were placed in tanks with 0.5 liter of water. Within each group, 3 subgroups consisting of 8 ovitraps were formed: 1 control without DFB and 2 with DFB at the concentration of 0.5 ppm and 0.25 ppm, respectively. Eight (4+4) controls without eggs were also added to assess the degradation over time of both DFB concentrations in absence of larvae. The level of water was maintained constant for the duration of the trial. The larvae were fed ad libitum with dried Gammarus pulex. Hatchings, development times, mortality and emerging adults were monitored for 8 weeks. In this period, DFB concentration was assessed every ten days by HPLC UV-DAD method after liquid-liquid extraction with diethyl ether. The number of eggs is significantly related with the last-hatching time and the time of last adult emergence (Negative binomial regression, p