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AbstractNine sampling units for estimating southern pine beetle population within a tree are compared according to unit size, strata size, and number and type of sampling allocation. As a measure of performance of different sampling schemes or different unit sizes the ratio of the variances of the estimate is used, i.e. relative efficiency.Relative efficiency decreases as unit size increases, where equal surface area is sampled. Unequal stratification, which allocates smaller area to the upper and lower strata, results in higher relative efficiencies than equal stratification. Thus, unequal stratification is recommended. Samples should be allocated to these strata according to the optimal sampling ratios.