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Background: Staphylococcus pseudintermedius, commonly involved in canine pyoderma, can be classified as meticillin-susceptible S. pseudintermedius (MSSP) or meticillin-resistant S. pseudintermedius (MRSP). MRSP infections may be difficult to treat due to broad β-lactam resistance of MRSP and typically additional multidrug-resistance. Topical antibacterial treatment is the preferred treatment modality for surface and superficial skin infections. HYPOTHESIS⁄OBJECTIVES: Mangosteen crude extract containing the antibacterial compound α-mangostin will have in vitro activity against MSSP and MRSP isolated from canine pyoderma., Bacterial Isolates: Twenty-three samples, MSSP (n = 12) and MRSP (n = 11), isolated from canine pyoderma., Methods and Materials: Minimum inhibitory concentrations (MICs) were determined for mangosteen crude extract by broth microdilution. High-performance liquid chromatography (HPLC) analysis was used to determine the amount of α-mangostin in mangosteen crude extract. A time-kill assay was performed at 30 min and 2 h after exposure to a high concentration of crude extract (100× MIC). Antibacterial activity for α-mangostin was calculated according to HPLC results., Results: The concentration of α-mangostin was 17.72 ± 1.42% w/w. The mean MIC of α-mangostin towards MSSP was 0.53 ± 0.35 μg/mL, whereas the mean value for MRSP was 0.47 ± 0.27 μg/mL. There was no difference between the mean MIC of MRSP and MSSP (P = 0.84). After a 30 min exposure to 100× MIC of the crude extract, a 95% reduction in colony forming units was found., Conclusions and Clinical Importance: The results showed that α-mangostin in mangosteen crude extract was effective in inhibiting S. pseudintermedius (both MRSP and MSSP). Clinical studies are needed to investigate this effectiveness further in vivo., (© 2019 ESVD and ACVD.)

Background: Generalized demodicosis in dogs can be challenging to manage, especially in adult-onset cases., Hypothesis/objectives: This study evaluated the efficacy of oral fluralaner at the dose of 25-50 mg/kg for the treatment of canine generalized demodicosis., Animals: Client-owned dogs from Bangkok, Thailand, diagnosed with generalized demodicosis according to published criteria., Methods: Deep skin scrapings were performed at three to five affected areas to evaluate numbers of mites. Repeat examinations were performed monthly until parasitological cure; dogs were then followed up for two to 12 months. Parasitological cure was defined as two negative skin scrapings achieved one month apart., Results: One hundred and fifteen dogs were included in the study, 73 with adult-onset demodicosis and 42 with juvenile-onset demodicosis. Twenty one dogs were lost to follow-up and 27 had one negative skin scraping but did not return. Sixty seven dogs (21 with juvenile-onset and 46 with adult-onset disease) reached parasitological cure, which occurred after two, three and four months in 63%, 85% and 100% (respectively) of dogs with adult-onset demodicosis, cumulatively, and after two and three months in 81% and 100% of dogs with juvenile-onset demodicosis. Underlying causes associated with adult-onset demodicosis included atopic dermatitis, neoplasia, metabolic diseases and idiopathy. No adverse effects of fluralaner were observed in any of the dogs., Conclusion: Fluralaner given at the label dose for flea and tick prevention is effective for the treatment of canine generalized demodicosis., (© 2018 ESVD and ACVD.)

This is a case report of concurrent of blastomycosis and pseudomycetoma in a 3 year-old Persian cat from Bangkok, Thailand. Histopathology from antemortem and postmortem samples revealed blastomycosis and dermatophyte pseudomycetoma. The PCR analysis of the formalin-embedded tissue of antemortem sample confirmed that blastomycosis was caused by Blastomyces dermatitidis. Dermatophyte infection was caused by Microsporum canis . According to the author's knowledge, this is the first case of Blastomyces dermatitidis and dermatophyte pseudomycetoma in South-East Asia.