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5 results on '"Larry, Blyn"'

1. AOAC SMPR 2016.010:Standard Method Performance Requirements (SMPRs) for DNA-Based Methods of Detecting Burkholderia pseudomallei in Field-Deployable, Department of Defense Aerosol Collection Devices

Author

Frank W. Schaefer, Apichai Tuanyok, Jennifer S. Arce, Thomas R. Blank, Jay E. Gee, Malcolm Johns, Paul Keim, Francisco F. Roberto, Bart J. Currie, Jason W. Sahl, Matthew Davenport, Larry Blyn, Stephen A. Morse, Katalin Kiss, David DeShazer, Matthew Lesho, Ryan Cahall, Nancy J. Lin, Linda Beck, Rich Ozanich, Pejman Naraghi-Arani, Amanda J. Clark, Herbert P. Schweizer, David A. Rozak, Steven E. Schutzer, Shanmuga Sozhamannan, Kenneth Damer, and Scott G Coates

Subjects
Pharmacology, biology, Burkholderia pseudomallei, 030231 tropical medicine, 030206 dentistry, Computational biology, biology.organism_classification, Analytical Chemistry, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Environmental Chemistry, Agronomy and Crop Science, Food Science
Published
2017
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2. AOAC SMPR 2016.012:Standard Method Performance Requirements (SMPRs) for Detection and Identification of Variola Virus

Author

Frank W. Schaefer, Kenneth Damer, Scott G Coates, Pejman Naraghi-Arani, Amanda J. Clark, Jennifer S. Arce, Mohamed Sofi Ibrahim, Paul J. Jackson, Ryan Cahall, Linda Beck, Elizabeth A. Vitalis, Larry Blyn, Katalin Kiss, Mark Scheckelhoff, Shanmuga Sozhamannan, Hermann Meyer, Victoria A. Olson, and Denise Pettit

Subjects
Pharmacology, Information retrieval, Computer science, Environmental Chemistry, Agronomy and Crop Science, Food Science, Analytical Chemistry
Published
2017
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3. AOAC SMPR 2016.010

Author

Jay, Gee, Jennifer, Arce, Linda C, Beck, Thomas R, Blank, Larry, Blyn, Ryan, Cahall, Amanda J, Clark, Bart, Currie, Kenneth, Damer, Matthew, Davenport, David, DeShazer, Malcolm, Johns, Paul S, Keim, Katalin, Kiss, Matthew, Lesho, Nancy, Lin, Stephen A, Morse, Pejman, Naraghi-Arani, Rich, Ozanich, Frank, Roberto, David, Rozak, Jason, Sahl, Frank, Schaefer, Steven, Schutzer, Herbert P, Schweizer, Shanmuga, Sozhamannan, Apichai, Tuanyok, and Scott, Coates

Published
2017

4. AOAC SMPR 2016.012

Author

Victoria, Olson, Jennifer, Arce, Linda C, Beck, Larry, Blyn, Ryan, Cahall, Amanda J, Clark, Kenneth, Damer, Mohamed Sofi, Ibrahim, Paul J, Jackson, Katalin, Kiss, Hermann, Meyer, Pejman, Naraghi-Arani, Denise, Pettit, Frank, Schaefer, Mark, Scheckelhoff, Shanmuga, Sozhamannan, Elizabeth, Vitalis, and Scott G, Coates

Published
2017

5. Interaction between pap-encoded pilin and adhesin gene products of uropathogenic Escherichia coli

Author

Larry Blyn and David A. Low

Subjects
Microbiology (medical), Enzyme-Linked Immunosorbent Assay, medicine.disease_cause, Pilus, Bacterial Adhesion, Microbiology, Plasmid, Gene cluster, medicine, Escherichia coli, Humans, Gene, Escherichia coli Infections, Adhesins, Escherichia coli, biology, Molecular biology, Immunohistochemistry, Precipitin Tests, Bacterial adhesin, Transformation (genetics), Microscopy, Electron, Infectious Diseases, Genes, Bacterial, Pilin, Fimbriae, Bacterial, Urinary Tract Infections, biology.protein, Fimbriae Proteins, Bacterial Outer Membrane Proteins, Plasmids
Abstract

Uropathogenic Escherichia coli are major causative agents of cystitis and pyelonephritis. Most E. coli pyelonephritis isolates express pili encoded by the pyelonephritis-associated pili (pap) gene cluster. The pap DNA sequence encodes pilin monomers that are assem- bled into pili fibers; pap also encodes adhesins that recent results suggest might be lo- cated at the tips of the pilus fibers. The study presented here is a status report of work that has two major goals: to determine (1) if any Pap proteins associate with pili and (2) if Pap proteins not required for pili assembly affect levels of pili-cell surface expres- sion. To address the first aim, antisera to pili were used to precipitate pili from detergent extracts containing 35S-labeled Pap proteins. The results suggested that a protein of 16- kilodaltons apparent molecular mass associated with pili. Other interpretations of the data are discussed. The second aim was addressed by constructing E. coli strains that contained different pap regions. With the use of electron microscopy and a pili ELISA, it was found that E. coli containing a 6.5-kilobase-pair region of pap expressed low levels of pili, but no P-adhesin was detected. Transformation of this E. coli strain with a plasmid containing an additional 3.5-kilobase-pair pap DNA sequence resulted in an eightfold increase in pili expression as well as expression of P adhesin. These results indicated that pili expression was affected by Pap proteins not required for pili assembly.

Published
1988

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