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1. L-Arginine and Taurisolo ® Effects on Brain Hypoperfusion–Reperfusion Damage in Hypertensive Rats.

Author

Lapi, Dominga, Tenore, Gian Carlo, Federighi, Giuseppe, Chiurazzi, Martina, Nunziato, Santo, Lonardo, Maria S., Stornaiuolo, Mariano, Colantuoni, Antonio, Novellino, Ettore, and Scuri, Rossana

Subjects
*BLOOD pressure, *CEREBRAL circulation, *LABORATORY rats, *CAROTID artery, *DIETARY supplements, *REPERFUSION
Abstract

Acute and chronic hypertension causes cerebral vasculopathy, increasing the risk of ischemia and stroke. Our study aimed to compare the effects of arterial pressure reduction on the pial microvascular responses induced by hypoperfusion and reperfusion in spontaneously hypertensive Wistar rats, desamethasone-induced hypertensive Wistar rats and age-matched normotensive Wistar rats fed for 3 months with a normal diet or normal diet supplemented with L-arginine or Taurisolo® or L-arginine plus Taurisolo®. At the end of treatments, the rats were submitted to bilateral occlusion of common carotid arteries for 30 min and reperfusion. The microvascular parameters investigated in vivo through a cranial window were: arteriolar diameter changes, permeability increase, leukocyte adhesion to venular walls and percentage of capillaries perfused. Hypoperfusion–reperfusion caused in all rats marked microvascular changes. L-arginine treatment was effective in reducing arterial blood pressure causing vasodilation but did not significantly reduce the damage induced by hypoperfusion–reperfusion. Taurisolo® treatment was less effective in reducing blood pressure but prevented microvascular damage from hypoperfusion–reperfusion. L-arginine plus Taurisolo® maintained blood pressure levels within the physiological range and protected the pial microcirculation from hypoperfusion–reperfusion-induced microvascular injuries. Therefore, the blood pressure reduction is not the only fundamental aspect to protect the cerebral circulation from hypoperfusion–reperfusion damage. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Biological Oscillations of Vascular Origin and Their Meaning: In Vivo Studies of Arteriolar Vasomotion

Author

Colantuoni, Antonio, Lapi, Dominga, Abarbanel, Henry D. I., Series Editor, Braha, Dan, Series Editor, Érdi, Péter, Series Editor, Friston, Karl J., Series Editor, Haken, Hermann, Series Editor, Jirsa, Viktor, Series Editor, Kacprzyk, Janusz, Series Editor, Kaneko, Kunihiko, Series Editor, Kelso, Scott, Founding Editor, Kirkilionis, Markus, Series Editor, Kurths, Jürgen, Series Editor, Menezes, Ronaldo, Series Editor, Nowak, Andrzej, Series Editor, Qudrat-Ullah, Hassan, Series Editor, Reichl, Linda, Series Editor, Schuster, Peter, Series Editor, Schweitzer, Frank, Series Editor, Sornette, Didier, Series Editor, Thurner, Stefan, Series Editor, Stefanovska, Aneta, editor, and McClintock, Peter V. E., editor

Published
2021
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4. The Anti-Inflammatory and Antioxidant Properties of Acebuche Oil Exert a Retinoprotective Effect in a Murine Model of High-Tension Glaucoma

Author

Universidad de Sevilla. Departamento de Fisiología, Italian Ministry of Universities and Research, University of Pisa, Lucchesi, Martina, Marracci, Silvia, Amato, Rosario, Lapi, Dominga, Santana Garrido, Álvaro, Espinosa Martín, Pablo, Vázquez Cueto, Carmen María, Mate Barrero, Alfonso, Dal Monte, Massimo, Universidad de Sevilla. Departamento de Fisiología, Italian Ministry of Universities and Research, University of Pisa, Lucchesi, Martina, Marracci, Silvia, Amato, Rosario, Lapi, Dominga, Santana Garrido, Álvaro, Espinosa Martín, Pablo, Vázquez Cueto, Carmen María, Mate Barrero, Alfonso, and Dal Monte, Massimo

Abstract

Glaucoma is characterized by cupping of the optic disc, apoptotic degeneration of retinal ganglion cells (RGCs) and their axons, and thinning of the retinal nerve fiber layer, with patchy loss of vision. Elevated intraocular pressure (IOP) is a major risk factor for hypertensive glaucoma and the only modifiable one. There is a need to find novel compounds that counteract other risk factors contributing to RGC degeneration. The oil derived from the wild olive tree (Olea europaea var. sylvestris), also called Acebuche (ACE), shows powerful anti-inflammatory, antioxidant and retinoprotective effects. We evaluated whether ACE oil could counteract glaucoma-related detrimental effects. To this aim, we fed mice either a regular or an ACE oil-enriched diet and then induced IOP elevation through intraocular injection of methylcellulose. An ACE oil-enriched diet suppressed glaucoma-dependent retinal glia reactivity and inflammation. The redox status of the glaucomatous retinas was restored to a control-like situation, and ischemia was alleviated by an ACE oil-enriched diet. Notably, retinal apoptosis was suppressed in the glaucomatous animals fed ACE oil. Furthermore, as shown by electroretinogram analyses, RGC electrophysiological functions were almost completely preserved by the ACE oil-enriched diet. These ameliorative effects were IOP-independent and might depend on ACE oil’s peculiar composition. Although additional studies are needed, nutritional supplementation with ACE oil might represent an adjuvant in the management of glaucoma.

Published
2024

5. The Anti-Inflammatory and Antioxidant Properties of Acebuche Oil Exert a Retinoprotective Effect in a Murine Model of High-Tension Glaucoma

Author

Ministero dell'Istruzione, dell'Università e della Ricerca, Università di Pisa, Santana-Garrido, Álvaro [0000-0002-9469-3850], Vázquez, Carmen M. [0000-0002-2999-3582], Mate, Alfonso [0000-0002-2719-8825], Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72], Lucchesi, Martina, Marracci, Silvia, Amato, Rosario, Lapi, Dominga, Santana-Garrido, Álvaro, Espinosa-Martín, Pablo, Vázquez, Carmen M., Mate, Alfonso, Dal Monte, Massimo, Ministero dell'Istruzione, dell'Università e della Ricerca, Università di Pisa, Santana-Garrido, Álvaro [0000-0002-9469-3850], Vázquez, Carmen M. [0000-0002-2999-3582], Mate, Alfonso [0000-0002-2719-8825], Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72], Lucchesi, Martina, Marracci, Silvia, Amato, Rosario, Lapi, Dominga, Santana-Garrido, Álvaro, Espinosa-Martín, Pablo, Vázquez, Carmen M., Mate, Alfonso, and Dal Monte, Massimo

Abstract

Glaucoma is characterized by cupping of the optic disc, apoptotic degeneration of retinal ganglion cells (RGCs) and their axons, and thinning of the retinal nerve fiber layer, with patchy loss of vision. Elevated intraocular pressure (IOP) is a major risk factor for hypertensive glaucoma and the only modifiable one. There is a need to find novel compounds that counteract other risk factors contributing to RGC degeneration. The oil derived from the wild olive tree (Olea europaea var. sylvestris), also called Acebuche (ACE), shows powerful anti-inflammatory, antioxidant and retinoprotective effects. We evaluated whether ACE oil could counteract glaucoma-related detrimental effects. To this aim, we fed mice either a regular or an ACE oil-enriched diet and then induced IOP elevation through intraocular injection of methylcellulose. An ACE oil-enriched diet suppressed glaucoma-dependent retinal glia reactivity and inflammation. The redox status of the glaucomatous retinas was restored to a control-like situation, and ischemia was alleviated by an ACE oil-enriched diet. Notably, retinal apoptosis was suppressed in the glaucomatous animals fed ACE oil. Furthermore, as shown by electroretinogram analyses, RGC electrophysiological functions were almost completely preserved by the ACE oil-enriched diet. These ameliorative effects were IOP-independent and might depend on ACE oil’s peculiar composition. Although additional studies are needed, nutritional supplementation with ACE oil might represent an adjuvant in the management of glaucoma.

Published
2024

9. β3-Adrenoceptor as a new player in the sympathetic regulation of the renal acid--base homeostasis.

Author

Milano, Serena, Saponara, Ilenia, Gerbino, Andrea, Lapi, Dominga, Lela, Ludovica, Carmosino, Monica, Monte, Massimo Dal, Bagnoli, Paola, Svelto, Maria, and Procino, Giuseppe

Subjects
HOMEOSTASIS, NERVE fibers, SALINE waters, CELL membranes, EPITHELIAL cells, ANDROGEN receptors
Abstract

Efferent sympathetic nerve fibers regulate several renal functions activating norepinephrine receptors on tubular epithelial cells. Of the betaadrenoceptors (β-ARs), we previously demonstrated the renal expression of β3-AR in the thick ascending limb (TAL), the distal convoluted tubule (DCT), and the collecting duct (CD), where it participates in salt and water reabsorption. Here, for the first time, we reported β3-AR expression in the CD intercalated cells (ICCs), where it regulates acid--base homeostasis. Co-localization of β3-AR with either proton pump H+-ATPase or Cl--/HCO3 -- exchanger pendrin revealed β3-AR expression in type A, type B, non-A, and non-B ICCs in the mouse kidney. We aimed to unveil the possible regulatory role of β3-AR in renal acid--base homeostasis, in particular in modulating the expression, subcellular localization, and activity of the renal H+-ATPase, a key player in this process. The abundance of H+-ATPase was significantly decreased in the kidneys of β3- AR--/-- compared with those of β3-AR+/+ mice. In particular, H+-ATPase reduction was observed not only in the CD but also in the TAL and DCT, which contribute to acid--base transport in the kidney. Interestingly, we found that in in vivo, the absence of β3-AR reduced the kidneys' ability to excrete excess proton in the urine during an acid challenge. Using ex vivo stimulation of mouse kidney slices, we proved that the β3-AR activation promoted H+-ATPase apical expression in the epithelial cells of β3-AR-expressing nephron segments, and this was prevented by β3-AR antagonism or PKA inhibition. Moreover, we assessed the effect of β3-AR stimulation on H+-ATPase activity by measuring the intracellular pH recovery after an acid load in β3-AR-expressing mouse renal cells. Importantly, β3-AR agonism induced a 2.5-fold increase in H+-ATPase activity, and this effect was effectively prevented by β3-AR antagonism or by inhibiting either H+-ATPase or PKA. Of note, in urine samples from patients treated with a β3-AR agonist, we found that β3-AR stimulation increased the urinary excretion of H+-ATPase, likely indicating its apical accumulation in tubular cells. These findings demonstrate that β3-AR activity positively regulates the expression, plasma membrane localization, and activity of H+-ATPase, elucidating a novel physiological role of β3-AR in the sympathetic control of renal acid--base homeostasis. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Effects of physical exercise associated with a diet enriched with natural antioxidants on cerebral hypoperfusion and reperfusion injury in spontaneously hypertensive rats

Author

Lapi, Dominga, primary, Federighi, Giuseppe, additional, Lonardo, Maria Serena, additional, Chiurazzi, Martina, additional, Muscariello, Espedita, additional, Tenore, Giancarlo, additional, Colantuoni, Antonio, additional, Novellino, Ettore, additional, and Scuri, Rossana, additional

Published
2023
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12. Increased efficacy of dietary supplement containing wax ester-rich marine oil and xanthophylls in a mouse model of dry macular degeneration

Author

Melecchi, Alberto, Amato, Rosario, Lapi, Dominga, Dal Monte, Massimo, Rusciano, Dario, Bagnoli, Paola, and Cammalleri, Maurizio

Subjects
retinal thickness, Pharmacology, gliosis, omega-3 fatty acids, inflammation, calanus oil, carotenoids, oxidative stress, Pharmacology (medical)
Abstract

Age-related macular degeneration (AMD) is nowadays considered among the retinal diseases whose clinical management lacks established treatment approaches, mainly for its atrophic (dry) form. In this respect, the use of dietary patterns enriched in omega-3 and antioxidant xanthophylls has emerged as a promising approach to counteract dry AMD progression although the prophylactic potential of omega-3 of fish origin has been discussed. Whether enriched availability of omega-3 and xanthophylls may increase the effectiveness of diet supplementation in preventing dry AMD remains to be fully established. The present study aims at comparing the efficacy of an existing orally administered formulation based on lutein and fish oil, as a source of omega-3, with a novel formulation providing the combination of lutein and astaxanthin with Calanus oil (COil), which contains omega-3 together with their precursors policosanols. Using a mouse model of dry AMD based on subretinal injection of polyethylene glycol (PEG)-400, we assessed the comparative efficacy of both formulations on PEG-induced major hallmarks including oxidative stress, inflammation, glial reactivity and outer retinal thickness. Dietary supplementation with both mixtures has been found to exert a significant antioxidant and anti-inflammatory activity as reflected by the overall amelioration of the PEG-induced pathological hallmarks. Noteworthy, the formulation based on COil appeared to be more protective than the one based on fish oil, presumably because of the higher bioavailability of omega-3 in COil. These results support the use of dietary supplements combining omega-3 and xanthophylls in the prevention and treatment of AMD and suggest that the source of omega-3 might contribute to treatment efficacy.

Published
2022

13. The Effects of Angiotensin II or Angiotensin 1-7 on Rat Pial Microcirculation during Hypoperfusion and Reperfusion Injury: Role of Redox Stress

Author

Lapi, Dominga, primary, Cammalleri, Maurizio, additional, Dal Monte, Massimo, additional, Di Maro, Martina, additional, Santillo, Maria Rosaria, additional, Belfiore, Anna, additional, Nasti, Gilda, additional, Damiano, Simona, additional, Trio, Rossella, additional, Chiurazzi, Martina, additional, De Conno, Barbara, additional, Serao, Nicola, additional, Mondola, Paolo, additional, Colantuoni, Antonio, additional, and Guida, Bruna, additional

Published
2021
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14. List of Contributors

Author

Agrawal, Abhishek, primary, Arasho, Belachew, additional, Bayat, Mohammad, additional, Bharati, Sachidanand J., additional, Bohluli, Behnam, additional, Bohluli, Pedram, additional, Buchholz, Bruno, additional, Cappellani, Ronald B., additional, Chowdhury, Tumul, additional, Dyavanapalli, Jhansi, additional, Erne, Paul, additional, Sadr-Eshkevari, Pooyan, additional, Gelpi, Ricardo J., additional, Golanov, Eugene V., additional, Goyal, Keshav, additional, Koerbel, Andrei, additional, Laleva, Lili N., additional, Lapi, Dominga, additional, Lemaitre, Frédéric, additional, Mendelowitz, David, additional, Meuwly, Cyrill, additional, Orbach, Darren B., additional, Prabhakhar, Hemanshu, additional, Rajaee, Arezoo, additional, Sandu, Nora, additional, Schaller, Bernhard J., additional, Singh, Gyaninder P., additional, Spiriev, Toma Y., additional, Vahdati, Gelarah, additional, Vahdati, Seyed Aliakbar, additional, Varedi, Payam, additional, and Wang, Xin, additional

Published
2015
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19. Protective effects of Lactobacillus paracasei F19 in a rat model of oxidative and metabolic hepatic injury

Author

Nardone, Gerardo, Compare, Debora, Liguori, Eleonora, Di Mauro, Valentina, Rocco, Alba, Barone, Michele, Napoli, Anna, Lapi, Dominga, Iovene, Maria Rosaria, and Colantuoni, Antonio

Subjects
Lactobacillus -- Health aspects, Oxidative stress -- Physiological aspects, Oxidative stress -- Models, Liver diseases -- Physiological aspects, Liver diseases -- Models, Biological sciences
Abstract

The liver is susceptible to such oxidative and metabolic stresses as ischemia-reperfusion (I/R) and fatty acid accumulation. Probiotics are viable microorganisms that restore the gut microbiota and exert a beneficial effect on the liver by inhibiting bacterial enzymes, stimulating immunity, and protecting intestinal permeability. We evaluated Lactobacillus paracasei F19 (LP-F19), for its potential protective effect, in an experimental model of I/R (30 min ischemia and 60 min reperfusion) in rats fed a standard diet or a steatogen [methionine/choline-deficient (MCD)] diet. Both groups consisted of 7 sham-operated rats, 10 rats that underwent I/R, and 10 that underwent I/R plus 8 wk of probiotic dietary supplementation. In rats fed a standard diet, I/R induced a decrease in sinusoid perfusion (P < 0.001), severe liver inflammation, and necrosis besides an increase of tissue levels of malondialdehyde (P < 0.001), tumor necrosis factor-[alpha] (P < 0.001), interleukin (IL)-1[beta] (P < 0.001), and IL-6 (P < 0.001) and of serum levels of transaminase (P < 0.001) and lipopolysaccharides (P < 0.001) vs. sham-operated rats. I/R also induced a decrease in Bacterioides, Bifidobacterium, and Lactobacillus spps (P < 0.01, P < 0.001, and P < 0.001, respectively) and an increase in Enterococcus and Enterobacteriaceae (P < 0.01 and P < 0.001, respectively) on intestinal mucosa. The severity of liver and gut microbiota alterations induced by I/R was even greater in rats with liver inflammation and steatosis, i.e., MCD-fed animals. LP-F19 supplementation significantly reduced the harmful effects of I/R on the liver and on gut microbiota in both groups of rats, although the effect was slightly less in MCD-fed animals. In conclusion, LP-FI9 supplementation, by restoring gut microbiota, attenuated I/R-related liver injury, particularly in the absence of steatosis. ischemia-reperfusion; Lactobacillus paracasei F19; nonalcoholic fatty liver disease; probiotics doi: 10.1152/ajpgi.00188.2010.

Published
2010

32. Laser Speckle Imaging of Rat Pial Microvasculature during Hypoperfusion-Reperfusion Damage

Author

Mastantuono, Teresa, primary, Starita, Noemy, additional, Battiloro, Laura, additional, Di Maro, Martina, additional, Chiurazzi, Martina, additional, Nasti, Gilda, additional, Muscariello, Espedita, additional, Cesarelli, Mario, additional, Iuppariello, Luigi, additional, D’Addio, Gianni, additional, Gorbach, Alexander, additional, Colantuoni, Antonio, additional, and Lapi, Dominga, additional

Published
2017
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36. Dietary protein intake in sarcopenic obese older women

Author

Muscariello,Espedita, Nasti,Gilda, Siervo,Mario, Di Maro,Martina, Lapi,Dominga, D'Addio,Gianni, Colantuoni,Antonio, Muscariello,Espedita, Nasti,Gilda, Siervo,Mario, Di Maro,Martina, Lapi,Dominga, D'Addio,Gianni, and Colantuoni,Antonio

Abstract

Espedita Muscariello,1 Gilda Nasti,1 Mario Siervo,2 Martina Di Maro,1 Dominga Lapi,1 Gianni D’Addio,3 Antonio Colantuoni1 1Department of Clinical Medicine and Surgery, Federico II University of Naples, Naples, Italy; 2Human Nutrition Research Centre, Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK; 3IRCCS Salvatore Maugeri Foundation, Telese, Italy Objective: To determine the prevalence of sarcopenia in a population of obese older women and to assess the effect of a diet moderately rich in proteins on lean mass in sarcopenic obese older women.  Materials and methods: A total of 1,030 females, >65 years old, body mass index >30 kg/m2, were investigated about their nutritional status. Muscle mass (MM) was estimated according to the Janssen equation (MM =0.401× height2/resistance measured at 50 kHz +3.825× sex -0.071× age +5.102). Sarcopenia was defined according to the MM index, MM/height2 (kg/m2), as two standard deviations lower than the obesity-derived cutoff score (7.3 kg/m2). A food-frequency questionnaire was used to measure participants’ usual food intake during the previous 3 months. Moreover, a group of sarcopenic obese older women (n=104) was divided in two subgroups: the first (normal protein intake [NPI], n=50) administered with a hypocaloric diet (0.8 g/kg desirable body weight/day of proteins), and the second treated with a hypocaloric diet containing 1.2 g/kg desirable body weight/day of proteins (high protein intake [HPI], n=54), for 3 months. Dietary ingestion was estimated according to a daily food diary, self-administered, and three reports of nonconsecutive 24-hour recall every month during the follow-up.  Results: The 104 women were classified as sarcopenic. After dieting, significant reductions in body mass index were detected (NPI 30.7±1.3 vs 32.0±2.3 kg/m2, HPI 30.26±0.90 vs 31.05±2.90&

Published
2016

38. Malvidin’s Effects on Rat Pial Microvascular Permeability Changes Due to Hypoperfusion and Reperfusion Injury

Author

Lapi, Dominga, primary, Chiurazzi, Martina, additional, Di Maro, Martina, additional, Mastantuono, Teresa, additional, Battiloro, Laura, additional, Sabatino, Lina, additional, Ricci, Serena, additional, Di Carlo, Angelina, additional, Starita, Noemy, additional, Guida, Bruna, additional, Santillo, Mariarosaria, additional, and Colantuoni, Antonio, additional

Published
2016
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42. Neuroprotective Effect of VEGF-Mimetic Peptide QK in Experimental Brain Ischemia Induced in Rat by Middle Cerebral Artery Occlusion

Author

Pignataro, Giuseppe, primary, Ziaco, Barbara, additional, Tortiglione, Anna, additional, Gala, Rosaria, additional, Cuomo, Ornella, additional, Vinciguerra, Antonio, additional, Lapi, Dominga, additional, Mastantuono, Teresa, additional, Anzilotti, Serenella, additional, D’Andrea, Luca Domenico, additional, Pedone, Carlo, additional, di Renzo, Gianfranco, additional, Annunziato, Lucio, additional, and Cataldi, Mauro, additional

Published
2015
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