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6. Identification of Human SARS-CoV-2 Monoclonal Antibodies from Convalescent Patients Using EBV Immortalization

Author

Valgardsdottir, R, Cattaneo, I, Napolitano, G, Raglio, A, Spinelli, O, Salmoiraghi, S, Castilletti, C, Lapa, D, Capobianchi, M, Farina, C, Golay, J, Valgardsdottir R, Cattaneo I, Napolitano G, Raglio A, Spinelli O, Salmoiraghi S, Castilletti C, Lapa D, Capobianchi MR, Farina C, Golay J, Valgardsdottir, R, Cattaneo, I, Napolitano, G, Raglio, A, Spinelli, O, Salmoiraghi, S, Castilletti, C, Lapa, D, Capobianchi, M, Farina, C, Golay, J, Valgardsdottir R, Cattaneo I, Napolitano G, Raglio A, Spinelli O, Salmoiraghi S, Castilletti C, Lapa D, Capobianchi MR, Farina C, and Golay J

Abstract

We report the isolation of two human IgG1k monoclonal antibodies (mAbs) directed against the SARS-CoV-2 spike protein. These mAbs were isolated from two donors who had recovered from COVID-19 infection during the first pandemic peak in the Lombardy region of Italy, the first European and initially most affected region in March 2020. We used the method of EBV immortalization of purified memory B cells and supernatant screening with a spike S1/2 assay for mAb isolation. This method allowed rapid isolation of clones, with one donor showing about 7% of clones positive against spike protein, whereas the other donor did not produce positive clones out of 91 tested. RNA was extracted from positive clones 39–47 days post-EBV infection, allowing VH and VL sequencing. The same clones were sequenced again after a further 100 days in culture, showing that no mutation had taken place during in vitro expansion. The B cell clones could be expanded in culture for more than 4 months after EBV immortalization and secreted the antibodies stably during that time, allowing to purify mg quantities of each mAb for functional assays without generating recombinant proteins. Unfortunately, neither mAb had significant neutralizing activity in a virus infection assay with several different SARS-CoV-2 isolates. The antibody sequences are made freely available.

Published
2021

8. Prophylactic heparin and risk of orotracheal intubation or death in patients with mild or moderate COVID-19 pneumonia

Author

Vergori, A., Lorenzini, P., Cozzi-Lepri, A., Donno, D. R., Gualano, G., Nicastri, E., Iacomi, F., Marchioni, L., Campioni, P., Schinina, V., Cicalini, S., Agrati, C., Capobianchi, M. R., Girardi, E., Ippolito, G., Vaia, F., Petrosillo, N., Antinori, A., Taglietti, F., The ReCOVeRI Study Group: Abbonizio, M. A., Abdeddaim, A., Agostini, E., Albarello, F., Amadei, G., Amendola, A., Antonica, M. A., Antonini, M., Bartoli, T. A., Baldini, F., Barbaro, R., Bartolini, B., Bellagamba, R., Benigni, M., Bevilacqua, N., Biava, G., Bibas, M., Bordi, L., Bordoni, V., Boumis, E., Branca, M., Buonomo, R., Busso, D., Camici, M., Canichella, F., Capone, A., Caporale, C., Caraffa, E., Caravella, I., Carletti, F., Castilletti, C., Cataldo, A., Cerilli, S., Cerva, C., Chiappini, R., Chinello, P., Cianfarani, M. A., Ciaralli, C., Cimaglia, C., Cinicola, N., Ciotti, V., Colavita, F., Corpolongo, A., Cristofaro, M., Curiale, S., D'Abramo, A., Dantimi, C., De Angelis, A., De Angelis, G., De Palo, M. G., De Zottis, F., Di Bari, V., Di Lorenzo, R., Di Stefano, F., D'Offizi, G., Evangelista, F., Faraglia, F., Farina, A., Ferraro, F., Fiorentini, L., Frustaci, A., Fusetti, M., Fusto, M., Galati, V., Gagliardini, R., Galli, P., Garotto, G., Gaviano, I., Tekle, S. G., Giancola, M. L., Giansante, F., Giombini, E., Granata, G., Greci, M. C., Grilli, E., Grisetti, S., Iaconi, M., Iannicelli, G., Inversi, C., Lalle, E., Lamanna, M. E., Lanini, S., Lapa, D., Lepore, L., Libertone, R., Lionetti, R., Liuzzi, G., Loiacono, L., Lucia, A., Lufrani, F., Macchione, M., Maffongelli, G., Marani, A., Mariano, A., Marini, M. C., Maritti, M., Mastrobattista, A., Mastrorosa, I., Matusali, G., Mazzotta, V., Mencarini, P., Meschi, S., Messina, F., Micarelli, S., Mogavero, G., Mondi, A., Montalbano, M., Montaldo, C., Mosti, S., Murachelli, S., Musso, M., Nardi, M., Navarra, A., Nocioni, M., Noto, P., Noto, R., Oliva, A., Onnis, I., Ottou, S., Palazzolo, C., Pallini, E., Palmieri, F., Palombi, G., Pareo, C., Passeri, V., Pelliccioni, F., Penna, G., Petrecchia, A., Petrone, A., Pianura, E., Pinnetti, C., Pisciotta, M., Piselli, P., Pittalis, S., Pontarelli, A., Proietti, C., Puro, V., Ramazzini, P. M., Rianda, A., Rinonapoli, G., Rosati, S., Rubino, D., Rueca, M., Ruggeri, A., Sacchi, A., Sampaolesi, A., Sanasi, F., Santagata, C., Scarabello, A., Scarcia, S., Scognamiglio, P., Scorzolini, L., Stazi, G., Strano, G., Taibi, C., Taloni, G., Nardi, T., Tonnarini, R., Topino, S., Tozzi, M., Vairo, F., Valli, M. B., Vincenzi, L., Visco-Comandini, U., Vita, S., Vittozzi, P., Zaccarelli, M., Zanetti, A., Zito, S., Vergori, A., Lorenzini, P., Cozzi-Lepri, A., Donno, D. R., Gualano, G., Nicastri, E., Iacomi, F., Marchioni, L., Campioni, P., Schinina, V., Cicalini, S., Agrati, C., Capobianchi, M. R., Girardi, E., Ippolito, G., Vaia, F., Petrosillo, N., Antinori, A., Taglietti, F., Abbonizio, M. A., Abdeddaim, A., Agostini, E., Albarello, F., Amadei, G., Amendola, A., Antonica, M. A., Antonini, M., Bartoli, T. A., Baldini, F., Barbaro, R., Bartolini, B., Bellagamba, R., Benigni, M., Bevilacqua, N., Biava, G., Bibas, M., Bordi, L., Bordoni, V., Boumis, E., Branca, M., Buonomo, R., Busso, D., Camici, M., Canichella, F., Capone, A., Caporale, C., Caraffa, E., Caravella, I., Carletti, F., Castilletti, C., Cataldo, A., Cerilli, S., Cerva, C., Chiappini, R., Chinello, P., Cianfarani, M. A., Ciaralli, C., Cimaglia, C., Cinicola, N., Ciotti, V., Colavita, F., Corpolongo, A., Cristofaro, M., Curiale, S., D'Abramo, A., Dantimi, C., De Angelis, A., De Angelis, G., De Palo, M. G., De Zottis, F., Di Bari, V., Di Lorenzo, R., Di Stefano, F., D'Offizi, G., Evangelista, F., Faraglia, F., Farina, A., Ferraro, F., Fiorentini, L., Frustaci, A., Fusetti, M., Fusto, M., Galati, V., Gagliardini, R., Galli, P., Garotto, G., Gaviano, I., Tekle, S. G., Giancola, M. L., Giansante, F., Giombini, E., Granata, G., Greci, M. C., Grilli, E., Grisetti, S., Iaconi, M., Iannicelli, G., Inversi, C., Lalle, E., Lamanna, M. E., Lanini, S., Lapa, D., Lepore, L., Libertone, R., Lionetti, R., Liuzzi, G., Loiacono, L., Lucia, A., Lufrani, F., Macchione, M., Maffongelli, G., Marani, A., Mariano, A., Marini, M. C., Maritti, M., Mastrobattista, A., Mastrorosa, I., Matusali, G., Mazzotta, V., Mencarini, P., Meschi, S., Messina, F., Micarelli, S., Mogavero, G., Mondi, A., Montalbano, M., Montaldo, C., Mosti, S., Murachelli, S., Musso, M., Nardi, M., Navarra, A., Nocioni, M., Noto, P., Noto, R., Oliva, A., Onnis, I., Ottou, S., Palazzolo, C., Pallini, E., Palmieri, F., Palombi, G., Pareo, C., Passeri, V., Pelliccioni, F., Penna, G., Petrecchia, A., Petrone, A., Pianura, E., Pinnetti, C., Pisciotta, M., Piselli, P., Pittalis, S., Pontarelli, A., Proietti, C., Puro, V., Ramazzini, P. M., Rianda, A., Rinonapoli, G., Rosati, S., Rubino, D., Rueca, M., Ruggeri, A., Sacchi, A., Sampaolesi, A., Sanasi, F., Santagata, C., Scarabello, A., Scarcia, S., Scognamiglio, P., Scorzolini, L., Stazi, G., Strano, G., Taibi, C., Taloni, G., Nardi, T., Tonnarini, R., Topino, S., Tozzi, M., Vairo, F., Valli, M. B., Vincenzi, L., Visco-Comandini, U., Vita, S., Vittozzi, P., Zaccarelli, M., Zanetti, A., and Zito, S.

Subjects
Male, medicine.medical_treatment, Rome, Kaplan-Meier Estimate, 030204 cardiovascular system & hematology, Severity of Illness Index, 0302 clinical medicine, Retrospective Studie, Coagulopathy, Clinical endpoint, Intubation, Respiratory function, 030212 general & internal medicine, Multidisciplinary, Middle Aged, Medicine, Female, Human, medicine.medical_specialty, Patients, medicine.drug_class, Science, Low molecular weight heparin, Risk Assessment, Article, NO, 03 medical and health sciences, Internal medicine, Severity of illness, medicine, Intubation, Intratracheal, Humans, Retrospective Studies, Aged, business.industry, SARS-CoV-2, COVID-19, Thrombocytopenia, Retrospective cohort study, Heparin, Low-Molecular-Weight, medicine.disease, Respiration, Artificial, COVID-19 Drug Treatment, respiratory tract diseases, Pneumonia, Viral infection, business
Abstract

Prophylactic low molecular weight heparin (pLMWH) is currently recommended in COVID-19 to reduce the risk of coagulopathy. The aim of this study was to evaluate whether the antinflammatory effects of pLMWH could translate in lower rate of clinical progression in patients with COVID-19 pneumonia. Patients admitted to a COVID-hospital in Rome with SARS-CoV-2 infection and mild/moderate pneumonia were retrospectively evaluated. The primary endpoint was the time from hospital admission to orotracheal intubation/death (OTI/death). A total of 449 patients were included: 39% female, median age 63 (IQR, 50–77) years. The estimated probability of OTI/death for patients receiving pLMWH was: 9.5% (95% CI 3.2–26.4) by day 20 in those not receiving pLMWH vs. 10.4% (6.7–15.9) in those exposed to pLMWH; p-value = 0.144. This risk associated with the use of pLMWH appeared to vary by PaO2/FiO2 ratio: aHR 1.40 (95% CI 0.51–3.79) for patients with an admission PaO2/FiO2 ≤ 300 mmHg and 0.27 (0.03–2.18) for those with PaO2/FiO2 > 300 mmHg; p-value at interaction test 0.16. pLMWH does not seem to reduce the risk of OTI/death mild/moderate COVID-19 pneumonia, especially when respiratory function had already significantly deteriorated. Data from clinical trials comparing the effect of prophylactic vs. therapeutic dosage of LMWH at various stages of COVID-19 disease are needed.

Published
2021

9. Retention of Neutralizing response against SARS-CoV-2 Omicron variant in Sputnik V vaccinated individuals

Author

Lapa, D, primary, Grousova, DM, additional, Matusali, G, additional, Meschi, S, additional, Colavita, F, additional, Bettini, A, additional, Gramigna, G, additional, Francalancia, M, additional, Garbuglia, AR, additional, Girardi, E, additional, Puro, V, additional, Antinori, A, additional, Kovyrshina, AV, additional, Dolzhikova, IV, additional, Shcheblyakov, DV, additional, Tukhvatulin, AI, additional, Zubkova, OV, additional, Logunov, DY, additional, Naroditsky, BS, additional, Vaia, F, additional, and Gintsburg, AL, additional

Published
2022
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10. Minimally invasive fetal surgery for myelomeningocele: Preliminary report from a single center

Author

Carrabba, G, Macchini, F, Fabietti, I, Schisano, L, Meccariello, G, Campanella, R, Bertani, G, Locatelli, M, Boito, S, Porro, G, Gabetta, L, Picciolini, O, Cinnante, C, Triulzi, F, Ciralli, F, Mosca, F, Lapa, D, Leva, E, Rampini, P, Persico, N, Carrabba G., Macchini F., Fabietti I., Schisano L., Meccariello G., Campanella R., Bertani G., Locatelli M., Boito S., Porro G. A., Gabetta L., Picciolini O., Cinnante C., Triulzi F., Ciralli F., Mosca F., Lapa D. A., Leva E., Rampini P., Persico N., Carrabba, G, Macchini, F, Fabietti, I, Schisano, L, Meccariello, G, Campanella, R, Bertani, G, Locatelli, M, Boito, S, Porro, G, Gabetta, L, Picciolini, O, Cinnante, C, Triulzi, F, Ciralli, F, Mosca, F, Lapa, D, Leva, E, Rampini, P, Persico, N, Carrabba G., Macchini F., Fabietti I., Schisano L., Meccariello G., Campanella R., Bertani G., Locatelli M., Boito S., Porro G. A., Gabetta L., Picciolini O., Cinnante C., Triulzi F., Ciralli F., Mosca F., Lapa D. A., Leva E., Rampini P., and Persico N.

Abstract

OBJECTIVE Recent trials have shown the safety and benefits of fetoscopic treatment of myelomeningocele (MMC). The authors' aim was to report their preliminary results of prenatal fetoscopic treatment of MMC using a biocellulose patch, focusing on neurological outcomes, fetal and maternal complications, neonatal CSF leakage, postnatal hydrocephalus, and radiological outcomes. METHODS Preoperative assessment included clinical examination, ultrasound imaging, and MRI of the fetus. Patients underwent purely fetoscopic in utero MMC repair, followed by postoperative in utero and postnatal MRI. All participants received multidisciplinary follow-up. RESULTS Five pregnant women carrying fetuses affected by MMC signed informed consent for the fetoscopic treatment of the defect. The mean MMC size was 30.4 mm (range 19-49 mm). Defect locations were L1 (2 cases), L5 (2 cases), and L4 (1 case). Hindbrain herniation and ventriculomegaly were documented in all cases. The mean gestational age at surgery was 28.2 weeks (range 27.8-28.8 weeks). Fetoscopic repair was performed in all cases. The mean gestational age at delivery was 33.9 weeks (range 29.3-37.4 weeks). After surgery, reversal of hindbrain herniation was documented in all cases. Three newborns developed signs of hydrocephalus requiring CSF diversion. Neurological outcomes in terms of motor level were favorable in all cases, but a premature newborn died due to CSF infection and sepsis. CONCLUSIONS The authors' preliminary results suggest that fetoscopic treatment of MMC is feasible, reproducible, and safe for mothers and their babies. Neurological outcomes were favorable and similar to those in the available literature. As known, prematurity was the greatest complication.

Published
2019

11. SARS-CoV-2 isolation from ocular secretions of a patient with COVID-19 in Italy with prolonged viral RNA detection

Author

Colavita F., Lapa D., Carletti F., Lalle E., Bordi L., Marsella P., Nicastri E., Bevilacqua N., Giancola M. L., Corpolongo A., Ippolito G., Capobianchi M. R., Castilletti C., Abbonizio M. A., Agrati C., Albarello F., Amadei G., Amendola A., Antonini M., Barbaro R., Bartolini B., Benigni M., Bordoni V., Branca M., Campioni P., Caporale C., Caravella I., Chiappini R., Ciaralli C., Cristofaro M., Curiale S., D'Abramo A., Dantimi C., de Angelis A., de Angelis G., Di Lorenzo R., Di Stefano F., Ferraro F., Fiorentini L., Frustaci A., Galli P., Garotto G., Giansante F., Giombini E., Greci M. C., Lanini S., Lepore L., Lucia A., Lufrani F., Macchione M., Marani A., Marchioni L., Mariano A., Marini M. C., Maritti M., Matusali G., Meschi S., Messina F., Montaldo C., Murachelli S., Noto R., Palazzolo C., Pallini E., Passeri V., Pelliccioni F., Petrecchia A., Petrone A., Petrosillo N., Pianura E., Pisciotta M., Pittalis S., Proietti C., Puro V., Rinonapoli G., Rueca M., Sacchi A., Sanasi F., Santagata C., Scarcia S., Schinina V., Scognamiglio P., Scorzolini L., Stazi G., Vaia F., Vairo F., Valli M. B., Colavita, F., Lapa, D., Carletti, F., Lalle, E., Bordi, L., Marsella, P., Nicastri, E., Bevilacqua, N., Giancola, M. L., Corpolongo, A., Ippolito, G., Capobianchi, M. R., Castilletti, C., Abbonizio, M. A., Agrati, C., Albarello, F., Amadei, G., Amendola, A., Antonini, M., Barbaro, R., Bartolini, B., Benigni, M., Bordoni, V., Branca, M., Campioni, P., Caporale, C., Caravella, I., Chiappini, R., Ciaralli, C., Cristofaro, M., Curiale, S., D'Abramo, A., Dantimi, C., de Angelis, A., de Angelis, G., Di Lorenzo, R., Di Stefano, F., Ferraro, F., Fiorentini, L., Frustaci, A., Galli, P., Garotto, G., Giansante, F., Giombini, E., Greci, M. C., Lanini, S., Lepore, L., Lucia, A., Lufrani, F., Macchione, M., Marani, A., Marchioni, L., Mariano, A., Marini, M. C., Maritti, M., Matusali, G., Meschi, S., Messina, F., Montaldo, C., Murachelli, S., Noto, R., Palazzolo, C., Pallini, E., Passeri, V., Pelliccioni, F., Petrecchia, A., Petrone, A., Petrosillo, N., Pianura, E., Pisciotta, M., Pittalis, S., Proietti, C., Puro, V., Rinonapoli, G., Rueca, M., Sacchi, A., Sanasi, F., Santagata, C., Scarcia, S., Schinina, V., Scognamiglio, P., Scorzolini, L., Stazi, G., Vaia, F., Vairo, F., and Valli, M. B.

Subjects
Isolation (health care), Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Eye, NO, 03 medical and health sciences, 0302 clinical medicine, Internal Medicine, Medicine, Viral rna, 030304 developmental biology, 0303 health sciences, SARS-CoV-2, business.industry, COVID-19, RNA, Pneumonia, General Medicine, Conjunctivitis, medicine.disease, Letters: Observations, Virology, 3. Good health, Viral replication, 030221 ophthalmology & optometry, business, Viral load, COVID-19, Conjunctivitis, Eye, Pneumonia, SARS-CoV-2
Abstract

Background: Coronavirus disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that originated in China in December 2019, was recently recognized as pandemic threat by the World Health Organization, with the potential of rapidly overloading health care systems and causing substantial mortality worldwide (www.who.int/dg/speeches/detail/who-director-general -s-opening-remarks-at-the-media-briefing-on-covid-19 —11-march -2020). Human-to-human transmission occurs mainly through respiratory droplets, but other routes are under investigation, because SARS-CoV-2 has been detected in several body fluids (1). So far, few data are available on ocular samples from patients with COVID-19, although conjunctivitis has been occasionally reported among COVID-19 symptoms, similar to infections caused by other human coronaviruses (2, 3). During the SARS epidemic, eye exposure to infectious fluids was associated with an increased risk for SARS-CoV transmission to health care workers (3, 4). Although SARS-CoV RNA was occasionally found in ocular specimens during the early phase of illness, its infectivity is unknown (2, 3). With regard to COVID-19, unprotected ocular exposure was thought to be responsible for infections that occurred in the Wuhan Fever Clinic in January 2020 (3, 4); in addition, SARS-CoV-2 RNA was detected in conjunctival secretions collected from the only patient with conjunctivitis out of 30 patients with COVID-19 from a hospital in China (5). However, further studies are needed to evaluate the infectious potential of the SARS-CoV-2 RNA detected in the ocular specimens and to determine whether transmission may occur through ocular secretions (3, 4). Objective: To present the early detection of infectious SARS-CoV-2 in ocular fluids from a patient with the first confirmed case of COVID-19 in Italy, who had been hospitalized at the National Institute for Infectious Diseases “L. Spallanzani” (INMI) in Rome. Methods and Findings: The patient, a 65-year-old woman, travelled from Wuhan, China, to Italy on 23 January 2020 and was admitted on 29 January 2020, 1 day after symptom onset. At admission to the high isolation unit at INMI, she presented with nonproductive cough, sore throat, coryza, and bilateral conjunctivitis. She had no fever until day 4, when fever (38 °C), nausea, and vomiting began. Infection with SARS-CoV-2 was confirmed by performing real-time reverse transcription polymerase chain reaction (RT-PCR) assay on sputum samples (cycle threshold value [Ct], 16.1) on the admission day, followed by viral M gene sequencing (GenBank accession number MT008022), and virus isolation on Vero E6 cell line (2019-nCoV/Italy-INMI1). The full genome sequence was obtained from either clinical sample or culture isolate (GISAID accession numbers EPI_ISL_410545 and EPI_ISL_410546). At admission, no other respiratory infections were detected (QIAstat-Dx Respiratory Panel; Qiagen).

Published
2020

15. Reply

Author

Joyeux, L., primary, Danzer, E., additional, De Bie, F., additional, Russo, F. M., additional, Javaux, A., additional, Peralta, C. F. A., additional, De Salles, A. A. F., additional, Pastuszka, A., additional, Olejek, A., additional, Van Mieghem, T., additional, De Coppi, P., additional, Moldenhauer, J., additional, Whitehead, W. E., additional, Belfort, M. A., additional, Lapa, D. A., additional, Acacio, G. L., additional, Devlieger, R., additional, Hirose, S., additional, Farmer, D. L., additional, Van Calenbergh, F., additional, Adzick, N. S., additional, Johnson, M. P., additional, and Deprest, J., additional

Published
2020
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16. Learning curves of open and endoscopic fetal spina bifida closure: systematic review and meta‐analysis

Author

Joyeux, L., primary, De Bie, F., additional, Danzer, E., additional, Russo, F. M., additional, Javaux, A., additional, Peralta, C. F. A., additional, De Salles, A. A. F., additional, Pastuszka, A., additional, Olejek, A., additional, Van Mieghem, T., additional, De Coppi, P., additional, Moldenhauer, J., additional, Whitehead, W. E., additional, Belfort, M. A., additional, Lapa, D. A., additional, Acacio, G. L., additional, Devlieger, R., additional, Hirose, S., additional, Farmer, D. L., additional, Van Calenbergh, F., additional, Adzick, N. S., additional, Johnson, M. P., additional, and Deprest, J., additional

Published
2020
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18. Proceedings of the First Annual Meeting of the International Fetoscopic Myelomeningocele Repair Consortium

Author

Sanz Cortes, M., primary, Lapa, D. A., additional, Acacio, G. L., additional, Belfort, M., additional, Carreras, E., additional, Maiz, N., additional, Peiro, J. L., additional, Lim, F. Y., additional, Miller, J., additional, Baschat, A., additional, Sepulveda, G., additional, Davila, I., additional, Gielchinsky, Y., additional, Benifla, M., additional, Stirnemann, J., additional, Ville, Y., additional, Yamamoto, M., additional, Figueroa, H., additional, Simpson, L., additional, and Nicolaides, K. H., additional

Published
2019
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21. Molecular signature of the Ebola virus associated with the fishermen community outbreak in Aberdeen, Sierra Leone, in February 2015

Author

Capobianchi, M, Gruber, C, Carletti, F, Meschi, S, Castilletti, C, Vairo, F, Biava, M, Minosse, C, Strada, G, Portella, G, Miccio, R, Minardi, V, Rolla, L, Kamara, A, Chillemi, G, Desideri, A, Di Caro, A, Ippolito, G, Cannas, A, Venditti, C, Zaccaro, P, Colavita, F, Mazzarelli, A, Vulcano, A, Chiappini, R, Valli, M, Grassi, G, Lapa, D, Quartu, S, and Coen, S

Subjects
Ebola virus, Settore BIO/11, Outbreak, Subclade, Biology, Disease cluster, Bioinformatics, medicine.disease_cause, Virology, 3. Good health, Sierra leone, Viruses, Genetics, medicine, Molecular Biology, Health worker
Abstract

We report the complete genome sequence of Ebola virus from a health worker linked to a cluster of cases occurring in the fishing community of Aberdeen, Sierra Leone (February 2015), which were characterized by unusually severe presentation. The sequence, clustering in the SL subclade 3.2.4, harbors mutations potentially relevant for pathogenesis.

Published
2015

22. Proceedings of the First Annual Meeting of the International Fetoscopic Myelomeningocele Repair Consortium.

Author

Cortes, M. Sanz, Lapa, D. A., Acacio, G. L., Belfort, M., Carreras, E., Maiz, N., Peiro, J. L., Lim, F. Y., Miller, J., Baschat, A., Sepulveda, G., Davila, I., Gielchinsky, Y., Benifla, M., Stirnemann, J., Ville, Y., Yamamoto, M., Figueroa, H., Simpson, L., and Nicolaides, K. H.

Subjects
*MYELOMENINGOCELE, *UTERINE rupture, *ABORTION, *OBSTETRICS, *ANNUAL meetings, *CONSORTIA
Abstract

Information about the fetoscopic myelomeningocele (MMC) repair techniques that were discussed at the first annual meeting of the International Fetoscopic MMC Repair Consortium is presented. Topics covered include fetal surgery by a totally percutaneous fetoscopic technique, neurosurgical and motor information for infants presented by six most experienced groups, and exteriorized uterus fetoscopic single-/double/triple-layer closure through two or three ports.

Published
2019
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46. Reply.

Author

Lapa, D. A., de Souza Leão, J. V., and coauthors

Subjects
*CEREBROSPINAL fluid shunts, *INTERMITTENT urinary catheterization, *SPINA bifida, *NEWBORN infants, *BLADDER, *QUALITY of work life
Abstract

We thank Prof. Macedo Jr and Dr Leal da Cruz for their comments on our study1. In the conservative approach, expectant management is adopted, with close surveillance based on dynamic ultrasound examination in which, in addition to assessment of the kidneys, bladder thickness, bladder trabeculation and urinary residue are measured. Percutaneous fetoscopic spina bifida repair: effect on ambulation and need for postnatal cerebrospinal fluid diversion and bladder catheterization. [Extracted from the article]

Published
2021
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47. Retrospective screening of solid organ donors in Italy, 2009, reveals unpredicted circulation of West Nile virus

Author

Capobianchi, M R, primary, Sambri, V, additional, Castilletti, C, additional, Pierro, A M, additional, Rossini, G, additional, Gaibani, P, additional, Cavrini, F, additional, Selleri, M, additional, Meschi, S, additional, Lapa, D, additional, Di Caro, A, additional, Grossi, P, additional, De Cillia, C, additional, Venettoni, S, additional, Landini, M P, additional, Ippolito, G, additional, Nanni Costa, A, additional, and on behalf of the Italian Transplant Network, Collective, additional

Published
2010
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49. Retrospective screening of solid organ donors in Italy, 2009, reveals unpredicted circulation of West Nile virus

Author

Capobianchi, Mr, Sambri, V, Castilletti, C, Pierro, Am, Rossini, G, Gaibani, P, Cavrini, F, Selleri, M, Meschi, S, Lapa, D, Di Caro, A, Grossi, P, De Cillia, C, Venettoni, S, Landini, Mp, Ippolito, G, Nanni Costa, A, Italian Transplant Network Collaborators: Scalamogna, M, Famulari, A, Saracino, A, Giacon, B, Mancini, P, Di Florio, E, Ridolfi, L, Peressutti, R, Adorno, D, Castiglione, Ag, Vesconi, S, Testasecca, D, Amoroso, Antonio, Schena, Fp, Carcassi, C, Sparacino, V, Lippi, R, Gabardi, E, Gambelunghe, C, Calabrò, F., Capobianchi M.R., Sambri V., Castilletti C., Pierro A.M., Rossini G., Gaibani P., Cavrini F., Selleri M., Meschi S., Lapa D., Di Caro A., Grossi P., De Cillia C., Venettoni S., Landini M., Ippolito G., and Nanni Costa A.

Subjects
SOLID ORGAN TRANSPLANT, Adult, organ donor, Epidemiology, West Nile virus, animal diseases, viruses, medicine.disease_cause, Virus, Flaviviridae, West nile virus, screening, transplantation, Predictive Value of Tests, Virology, medicine, Humans, Aged, Retrospective Studies, Aged, 80 and over, biology, business.industry, Transmission (medicine), SEROPREVALENCE, Public Health, Environmental and Occupational Health, virus diseases, Retrospective cohort study, Middle Aged, biology.organism_classification, Health Surveys, Tissue Donors, nervous system diseases, Transplantation, Flavivirus, Italy, Immunology, Risk assessment, business, West Nile Fever
Abstract

Since the occurrence of West Nile virus (WNV) infection in humans in 2008 in Italy, concerns have been raised about the potential risks associated with solid organ transplantation (SOT). A nationwide retrospective survey showed that 1.2% of SOT donors in 2009 were WNV-seropositive and demonstrated that human WNV infection is distributed throughout several Italian regions. Transmission of WNV or other arboviruses through SOT is a possibility and risk assessment should be carried out before SOT to avoid infection through transplantation.

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