Search

The infiltration of immune cells into sites of inflammation is one key feature of immune mediated inflammatory diseases. A detailed assessment of the in vivo dynamics of relevant cell subtypes could booster the understanding of this disease and the development of novel therapies. We show in detail how advanced X-ray fluorescence imaging enables such quantitative in vivo cell tracking, offering solutions that could pave the way beyond what other imaging modalities provide today. The key for this achievement is a detailed study of the spectral background contribution from multiple Compton scattering in a mouse-scaled object when this is scanned with a monochromatic pencil X-ray beam from a synchrotron. Under optimal conditions, the detection sensitivity is sufficient for detecting local accumulations of the labelled immune cells, hence providing experimental demonstration of in vivo immune cell tracking in mice. [ABSTRACT FROM AUTHOR]

The article focuses on how the identity of apoptotic cells influences macrophage responses to Interleukin 4 (IL-4). Topics discussed include the diverse functional responses of macrophages to apoptotic neutrophils, hepatocytes, and thymocytes, as well as the role of phagocytic receptors AXL and MERTK in this process.

A series of forty-six 5,6-annulated 2-arylthieno [2,3- d pyrimidin-4(3 H)-ones were prepared as potentially pleiotropic anticancer drugs with variance in the tubulin-binding trimethoxyphenyl motif at C-2 of a thieno [2,3- d pyrimidine fragment, enlarged by additional rings of different size and substitution. By assessing their cytotoxicity against various cancer cells, their influence on the polymerization of neat tubulin and the dynamics of microtubule and F-actin cytoskeletons, and their vascular-disrupting and anti-angiogenic activities in vitro and in vivo , structure-activity relations were identified which suggest the 3-iodo-4,5-dimethoxyphenyl substituted thienopyrimidine 2e as a promising anticancer drug candidate for further research. 2020 Elsevier Ltd. All rights reserved. Madeleine Gold, Leonhard Köhler, Clarissa Lanzloth, Ion Andronache, Shrikant Anant, Prasad Dandawate, Bernhard Biersack, Rainer Schobert. Image 1 [ABSTRACT FROM AUTHOR]