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2. Changes in DNA methylation contribute to rapid adaptation in bacterial plant pathogen evolution.

Author

Gopalan-Nair, Rekha, Coissac, Aurore, Legrand, Ludovic, Lopez-Roques, Céline, Pécrix, Yann, Vandecasteele, Céline, Bouchez, Olivier, Barlet, Xavier, Lanois, Anne, Givaudan, Alain, Brillard, Julien, Genin, Stéphane, and Guidot, Alice

Subjects
DNA analysis, AGRICULTURE, BACTERIAL adaptation, GENETIC mutation, GENE expression
Abstract

Adaptation is usually explained by beneficial genetic mutations that are transmitted from parents to offspring and become fixed in the adapted population. However, genetic mutation analysis alone is not sufficient to fully explain the adaptive processes, and several studies report the existence of nongenetic (or epigenetic) inheritance that can enable adaptation to new environments. In the present work, we tested the hypothesis of the role of DNA methylation, a form of epigenetic modification, in adaptation of the plant pathogen Ralstonia pseudosolanacearum to the host during experimental evolution. Using SMRT-seq technology, we analyzed the methylomes of 31 experimentally evolved clones obtained after serial passages on 5 different plant species during 300 generations. Comparison with the methylome of the ancestral clone revealed a list of 50 differential methylated sites (DMSs) at the GTWWAC motif. Gene expression analysis of the 39 genes targeted by these DMSs revealed limited correlation between differential methylation and differential expression of the corresponding genes. Only 1 gene showed a correlation, the RSp0338 gene encoding the EpsR regulator protein. The MSRE-qPCR technology, used as an alternative approach for DNA methylation analysis, also found the 2 DMSs upstream RSp0338. Using site-directed mutagenesis, we demonstrated the contribution of these 2 DMSs in host adaptation. As these DMSs appeared very early in the experimental evolution, we hypothesize that such fast epigenetic changes can allow rapid adaptation to the plant stem environment. In addition, we found that the change in DNA methylation upstream RSp0338 remains stable at least for 100 generations outside the host and thus can contribute to long-term adaptation to the host plant. To our knowledge, this is the first study showing a direct link between bacterial epigenetic variation and adaptation to a new environment. Do epigenetic changes contribute to long-term bacterial adaptation? This study shows that changes in DNA methylation enable rapid adaptation of the major agricultural bacterial pathogen Ralstonia pseudosolanacearum to novel hosts, stable over 100 generations. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Odilorhabdins, Antibacterial Agents that Cause Miscoding by Binding at a New Ribosomal Site

Author

Pantel, Lucile, Florin, Tanja, Dobosz-Bartoszek, Malgorzata, Racine, Emilie, Sarciaux, Matthieu, Serri, Marine, Houard, Jessica, Campagne, Jean-Marc, de Figueiredo, Renata Marcia, Midrier, Camille, Gaudriault, Sophie, Givaudan, Alain, Lanois, Anne, Forst, Steve, Aumelas, André, Cotteaux-Lautard, Christelle, Bolla, Jean-Michel, Vingsbo Lundberg, Carina, Huseby, Douglas L., Hughes, Diarmaid, Villain-Guillot, Philippe, Mankin, Alexander S., Polikanov, Yury S., and Gualtieri, Maxime

Published
2018
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4. Flagellar Regulation and Virulence in the Entomopathogenic Bacteria—Xenorhabdus nematophila and Photorhabdus luminescens

Author

Givaudan, Alain, Lanois, Anne, Compans, Richard W, Series editor, Honjo, Tasuku, Series editor, Oldstone, Michael B. A., Series editor, Vogt, Peter K., Series editor, Malissen, Bernard, Series editor, Aktories, Klaus, Series editor, Kawaoka, Yoshihiro, Series editor, Rappuoli, Rino, Series editor, Galan, Jorge E., Series editor, Ahmed, Rafi, Series editor, Palme, Klaus, Series editor, Casadevall, Arturo, Series editor, Garcia-Sastre, Adolfo, Series editor, and ffrench-Constant, Richard H., editor

Published
2017
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5. Evidence for increased fitness of a plant pathogen conferred by epigenetic variation

Author

Gopalan-Nair, Rekha, primary, Coissac, Aurore, additional, Legrand, Ludovic, additional, Lopez-Roques, Celine, additional, Pecrix, Yann, additional, Vandecasteele, Celine, additional, BOUCHEZ, Olivier, additional, Barlet, Xavier, additional, Lanois, Anne, additional, Givaudan, Alain, additional, Brillard, Julien, additional, Genin, Stephane, additional, and GUIDOT, Alice, additional

Published
2023
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11. Stages of infection during the tripartite interaction between Xenorhabdus nematophila, its nematode vector, and insect hosts

Author

Sicard, Mathieu, Brugirard-Ricaud, Karine, Pages, Sylvie, Lanois, Anne, Boemare, Noel E., Brehelin, Michel, and Givaudan, Alain

Subjects
Nematoda -- Research, Insect societies -- Research, Biological sciences
Abstract

The early steps of insect colonization by the bacterial symbiont by constructing a green fluorescent protein (GFP)-labeled Xenorhabdus nematophila strain are described. The extracelluar matrix acts as a particular colonization site for X. nematophila with insects.

Published
2004

14. Role of the Photorhabdus Dam methyltransferase during interactions with its invertebrate hosts

Author

Payelleville, Amaury, Blackburn, Dana, Lanois, Anne, Pages, Sylvie, C Cambon, Marine, Ginibre, Nadège, Clarke, David J., Givaudan, Alain, Brillard, Julien, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Department of Microbiology, University of Washington [Seattle], Evolution et Diversité Biologique (EDB), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, GAIA doctoral school (584), INRA Plant Health and Environment (SPE) division (SPE-IB17-DiscriMet), Académie d'Agriculture de France, French ministry MEAE, French ministry MESRI, ANR-17-CE20-0005,EPI-PATH,Rôle des modifications épigénétiques chez un pathogène de plante au cours de l'adaptation à l'hôte et des changements d'hôte(2017), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), and Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Site-Specific DNA-Methyltransferase (Adenine-Specific), Life Cycles, Insecta, Nematoda, Molecular biology, Psychologie appliquée, Pathogenesis, Pathology and Laboratory Medicine, Photorhabdus luminescens, Biochemistry, bacterial dna, Dam DNA, Larvae, Medicine and Health Sciences, Nematode Infections, bactérie entomopathogène, nématode, DNA methylation, Eukaryota, Sciences bio-médicales et agricoles, Chromatin, symbiosis, Bacterial Pathogens, facteur de pathogénicité, Nucleic acids, Insects, Medical Microbiology, Medicine, Epigenetics, Pathogens, Photorhabdus, DNA modification, symbiose, Biologie, Chromatin modification, Research Article, Chromosome biology, Cell biology, Arthropoda, Science, entomopathogenic bacteria, Nematode infections, DNA construction, Plasmid construction, Microbiology, Bacterial Proteins, Genetics, Parasitic Diseases, Animals, heterorhabditis, Symbiosis, Microbial Pathogens, photorhabdus luminescens, Organisms, Biology and Life Sciences, DNA, Bacterial DNA, Invertebrates, Research and analysis methods, Species Interactions, Molecular biology techniques, Plasmid Construction, adn bactérien, Bacterial pathogens, Gene expression, Dam methyltransferase, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Developmental Biology
Abstract

Photorhabdus luminescens is an entomopathogenic bacterium found in symbiosis with the nematode Heterorhabditis. Dam DNA methylation is involved in the pathogenicity of many bacteria, including P. luminescens, whereas studies about the role of bacterial DNA methylation during symbiosis are scarce. The aim of this study was to determine the role of Dam DNA methylation in P. luminescens during the whole bacterial life cycle including during symbiosis with H. bacteriophora. We constructed a strain overexpressing dam by inserting an additional copy of the dam gene under the control of a constitutive promoter in the chromosome of P. luminescens and then achieved association between this recombinant strain and nematodes. The dam overexpressing strain was able to feed the nematode in vitro and in vivo similarly as a control strain, and to re-associate with Infective Juvenile (IJ) stages in the insect. No difference in the amount of emerging IJs from the cadaver was observed between the two strains. Compared to the nematode in symbiosis with the control strain, a significant increase in LT50 was observed during insect infestation with the nematode associated with the dam overexpressing strain. These results suggest that during the life cycle of P. luminescens, Dam is not involved the bacterial symbiosis with the nematode H. bacteriophora, but it contributes to the pathogenicity of the nemato-bacterial complex., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2019
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15. Selection of Bacterial Mutants in Late Infections: When Vector Transmission Trades Off against Growth Advantage in Stationary Phase

Author

Cambon, Marine, Parthuisot, Nathalie, Pages, Sylvie, Lanois, Anne, Givaudan, Alain, Ferdy, Jean-Baptiste, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Evolution et Diversité Biologique (EDB), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
hôte, Biodiversité et Ecologie, Ecological and Evolutionary Science, Microbiology, Xenorhabdus, Biodiversity and Ecology, steinernema carpocapsae, Rhabditida, Animals, mutant, Selection, Genetic, diversité phénotypique, nématode, pouvoir pathogène, Microbiota, infection bactérienne, within-host evolution, transmission, Genetic Variation, Xenorhabdus nematophila, GASP, QR1-502, Insect Vectors, Biological Variation, Population, Mutation, xenorhabdus nematophilus, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Gram-Negative Bacterial Infections, Research Article
Abstract

Pathogens can evolve inside their host, and the importance of this mutation-fueled process is increasingly recognized. A disease outcome may indeed depend in part on pathogen adaptations that emerge during infection. It is therefore important to document these adaptations and the conditions that drive them. In our study, we took advantage of the possibility to monitor within-host evolution in the insect pathogen X. nematophila. We demonstrated that selection occurring in aged infection favors lrp defective mutants, because these metabolic mutants benefit from a growth advantage in stationary phase (GASP). We also demonstrated that these mutants have reduced virulence and impaired transmission, modifying the infection outcome. Beyond the specific case of X. nematophila, we propose that metabolic mutants are to be found in other bacterial pathogens that stay for many generations inside their host., Bacterial infections are often composed of cells with distinct phenotypes that can be produced by genetic or epigenetic mechanisms. This phenotypic heterogeneity has proved to be important in many pathogens, because it can alter both pathogenicity and transmission. We studied how and why it can emerge during infection in the bacterium Xenorhabdus nematophila, a pathogen that kills insects and multiplies in the cadaver before being transmitted by the soil nematode vector Steinernema carpocapsae. We found that phenotypic variants cluster in three groups, one of which is composed of lrp defective mutants. These mutants, together with variants of another group, have in common that they maintain high survival during late stationary phase. This probably explains why they increase in frequency: variants of X. nematophila with a growth advantage in stationary phase (GASP) are under strong positive selection both in prolonged culture and in late infections. We also found that the within-host advantage of these variants seems to trade off against transmission by nematode vectors: the variants that reach the highest load in insects are those that are the least transmitted.

Published
2019
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16. Salmonella typhimurium cobalamin (vitamin B12) biosynthetic genes: functional studies in S. typhimurium and Escherichia coli

Author

Raux, Evelyne, Lanois, Anne, Levillayer, Florence, Warren, Martin J., Brody, Edward, Ranbach, Alain, and Thermes, Claude

Subjects
Salmonella typhimurium -- Genetic aspects, Vitamin B12 metabolism -- Genetic aspects, Escherichia coli -- Genetic aspects, Biological sciences
Abstract

A study was conducted to investigate the Salmonella typhimurium biosynthetic pathway. The S. typhimurium cob operon was isolated and cloned into Escherichia coli to confer the ability to make cobalamins de novo. Genetic analyses of cobalamin E. coli-producing strains that were constructed on E. coli strains with inactivated cob operon revealed cbiD and cbiG or cobI genes. Mutation analysis showed that cobalt is required for the initial stages during cobalamin synthesis.

Published
1996

17. Spatiotemporal expression of the putative MdtABC efflux pump of Phtotorhabdus luminescens occurs in a protease-dependent manner during insect infection

Author

Abi Khattar, Ziad, Lanois, Anne, Hadchity, Linda, Gaudriault, Sophie, Givaudan, Alain, Laboratoryof Georesources, GeosciencesandEnvironment, Microbiologyteam, Faculty of Science 2, Lebanese University (Lebanese University), Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), University of Montpellier [0836], National Council for Scientific Research - Lebanon (CNRS-L) [02-05-2016], Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), and Lebanese University [Beirut] (LU)

Subjects
Life Cycles, Luminescence, Transcription, Genetic, Biodiversité et Ecologie, Artificial Gene Amplification and Extension, Biochemistry, Polymerase Chain Reaction, Larvae, Fluorescence Microscopy, Nucleic Acids, Medicine and Health Sciences, Nematode Infections, Promoter Regions, Genetic, nématode, Microscopy, Physics, Electromagnetic Radiation, fusion transcriptionnelle, Eukaryota, Light Microscopy, symbiosis, Anti-Bacterial Agents, Insects, Phenotype, Physical Sciences, Medicine, Photorhabdus, symbiose, Research Article, bacterial genes, Arthropoda, Science, gène bactérien, Microbial Sensitivity Tests, entérobactérie, Spodoptera, Research and Analysis Methods, Fluorescence, Biodiversity and Ecology, Bacterial Proteins, Operon, Genetics, Parasitic Diseases, Animals, mutant, Operons, Molecular Biology Techniques, Molecular Biology, photorhabdus luminescens, locusta migratoria, Gene Expression Profiling, fungi, Organisms, Biology and Life Sciences, DNA, Invertebrates, Mutation, transcriptional fusion, Genes, MDR, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Copper, Developmental Biology, Peptide Hydrolases
Abstract

Photorhabdus luminescens is an enterobacterium establishing a mutualistic symbiosis with nematodes, that also kills insects after septicaemia and connective tissue colonization. The role of the bacterial mdtABC genes encoding a putative multidrug efflux system from the resistance/nodulation/cell division family was investigated. We showed that a mdtA mutant and the wild type had similar levels of resistance to antibiotics, antimicrobial peptides, metals, detergents and bile salts. The mdtA mutant was also as pathogenic as the wild-type following intrahaemocoel injection in Locusta migratoria, but had a slightly attenuated phenotype in Spodoptera littoralis. A transcriptional fusion of the mdtA promoter (P-mdtA) and the green fluorescent protein (gfp) encoding gene was induced by copper in bacteria cultured in vitro. The P-mdtA-gfp fusion was strongly induced within bacterial aggregates in the haematopoietic organ during late stages of infection in L. migratoria, whereas it was only weakly expressed in insect plasma throughout infection. A medium supplemented with haematopoietic organ extracts induced the P-mdtA-gfp fusion ex vivo, suggesting that site-specific mdtABC expression resulted from insect signals from the haematopoietic organ. Finally, we showed that protease inhibitors abolished ex vivo activity of the P-mdtA-gfp fusion in the presence of haematopoietic organ extracts, suggesting that proteolysis by-products play a key role in upregulating the putative MdtABC efflux pump during insect infection with P. luminescens.

Published
2019
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18. Inhibition of Spodoptera frugiperda phenoloxidase activity by the products of the Xenorhabdus rhabduscin gene cluster

Author

Eugenia Nuñez-Valdez, Maria, Lanois, Anne, Pagès, Sylvie, Duvic, Bernard, Gaudriault, Sophie, Universidad Autonoma del Estado de Morelos (UAEM), Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), INRA [SPE 2010-1133-01], CONACYT [CB2014-243027], INRA, Universidad Autónoma del Estado de Morelos, and Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)

Subjects
Life Cycles, Hemocytes, Biodiversité et Ecologie, Gene Expression, Pathology and Laboratory Medicine, Biochemistry, Xenorhabdus, White Blood Cells, Larvae, Animal Cells, Nucleic Acids, Invertebrate Genomics, Medicine and Health Sciences, bactérie entomopathogène, Escherichia Coli, Monophenol Monooxygenase, Eukaryota, Genomics, Bacterial Pathogens, Insects, Experimental Organism Systems, Medical Microbiology, Multigene Family, Medicine, Prokaryotic Models, Insect Proteins, spodoptera, Cellular Types, Pathogens, Research Article, Escherichia, Arthropoda, Science, Immune Cells, entomopathogenic bacteria, Immunology, Research and Analysis Methods, Microbiology, Promoter Regions, Biodiversity and Ecology, Model Organisms, Enterobacteriaceae, Genetics, Animals, Gene Regulation, mutant, Pest Control, Biological, Microbial Pathogens, virulence bactérienne, Blood Cells, Bacteria, Gut Bacteria, fungi, Organisms, Biology and Life Sciences, Cell Biology, DNA, Invertebrates, Animal Genomics, Mutation, Animal Studies, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Developmental Biology
Abstract

We evaluated the impact of bacterial rhabduscin synthesis on bacterial virulence and phenoloxidase inhibition in a Spodoptera model. We first showed that the rhabduscin cluster of the entomopathogenic bacterium Xenorhabdus nematophila was not necessary for virulence in the larvae of Spodoptera littoralis and Spodoptera frugiperda. Bacteria with mutations affecting the rhabduscin synthesis cluster (ΔisnAB and ΔGT mutants) were as virulent as the wild-type strain. We then developed an assay for measuring phenoloxidase activity in S. frugiperda and assessed the ability of bacterial culture supernatants to inhibit the insect phenoloxidase. Our findings confirm that the X. nematophila rhabduscin cluster is required for the inhibition of S. frugiperda phenoloxidase activity. The X. nematophila ΔisnAB mutant was unable to inhibit phenoloxidase, whereas ΔGT mutants displayed intermediate levels of phenoloxidase inhibition relative to the wild-type strain. The culture supernatants of Escherichia coli and of two entomopathogenic bacteria, Serratia entomophila and Xenorhabdus poinarii, were unable to inhibit S. frugiperda phenoloxidase activity. Heterologous expression of the X. nematophila rhabduscin cluster in these three strains was sufficient to restore inhibition. Interestingly, we observed pseudogenization of the X. poinarii rhabduscin gene cluster via the insertion of a 120 bp element into the isnA promoter. The inhibition of phenoloxidase activity by X. poinarii culture supernatants was restored by expression of the X. poinarii rhabduscin cluster under the control of an inducible Ptet promoter, consistent with recent pseudogenization. This study paves the way for advances in our understanding of the virulence of several entomopathogenic bacteria in non-model insects, such as the new invasive S. frugiperda species in Africa.

Published
2019
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19. Photorhabdus Dam methyltransferase overexpression impairs virulence of the nemato-bacterial complex in insects

Author

Payelleville, Amaury, Blackburn, Dana, Lanois, Anne, Pages, Sylvie, Cambon, Marine C., Ginibre , Nadège, Clarke, David J., Givaudan, Alain, and Brillard, Julien

Subjects
Biodiversity and Ecology, Biodiversité et Ecologie, entérobactérie, lutte biologique, dam, nematobacterial complex, symbiosis, pathogenicity, bactérie pathogène, nématode, photorhabdus luminescens
Abstract

Photorhabdus luminescens is an entomopathogenic bacterium found in symbiosis with the nematode Heterorhabditis. Dam DNA methylation is involved in the pathogenicity of many bacteria, including P. luminescens, whereas studies about the role of bacterial DNA methylation during symbiosis are scarce. The aim of this study was to determine the role of Dam DNA methylation in P. luminescens symbiosis with H. bacteriophora. We constructed a strain overexpressing dam by inserting an additional copy of the dam gene under the control of a constitutive promoter in the chromosome of P. luminescens and then achieved association between this recombinant strain and nematodes. The dam overexpressing strain was able to feed the nematode in vitro and in vivo similarly as a control strain, and to re-associate with Infective Juvenile (IJ) stages in the insect. No difference in the amount of emerging IJs from the cadaver was observed between the two strains. Compared to the nematode in symbiosis with the control strain, a significant increase in LT50 was observed during insect infestation with the nematode associated with the dam overexpressing strain. These results suggest that the P. luminescens Dam plays a role in the pathogenicity of the nemato-bacterial complex.

Published
2019

25. An antimicrobial peptide-resistant minor subpopulation of Photorhabdus luminescens is responsible for virulence

Author

Mouammine, Annabelle, primary, Pages, Sylvie, additional, Lanois, Anne, additional, Gaudriault, Sophie, additional, Jubelin, Gregory, additional, Bonabaud, Maurine, additional, Cruveiller, Stéphane, additional, Dubois, Emeric, additional, Roche, David, additional, Legrand, Ludovic, additional, Brillard, Julien, additional, and Givaudan, Alain, additional

Published
2017
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27. Pathology survey and comparative genomics in the bacterial insect pathogen Xenorhabdus

Author

Gaudriault, Sophie, Ogier, Jean-Claude, Pages, Sylvie, Bisch, Gaelle, Ginibre, Nadege, Lanois, Anne, Tailliez, Patrick, Givaudan, Alain, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), and Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, entomopathogenic bacteria, xenorhabdus, education, fungi, entérobactérie, lutte biologique, symbiosis, biodiversité, stomatognathic diseases, otorhinolaryngologic diseases, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, symbiose, génomique des populations, bactérie entomopathogène, health care economics and organizations
Abstract

Pathology survey and comparative genomics in the bacterial insect pathogen Xenorhabdus . 5. Congress of European Microbiologists (FEMS 2013)

Published
2013

28. La protéine FliZ de Xenorhabdus nematophila : un régulateur pléiotropique

Author

Lanois, Anne, Gaudriault, Sophie, Jubelin, Grégory, Gouzy, Jerome, Severac, Dany, Rialle, Stéphanie, Longin, Cyrille, Givaudan, Alain, Diversité, Génomes et Interactions Microorganismes-Insectes, Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Unité de Microbiologie (MIC), Institut National de la Recherche Agronomique (INRA), Unité mixte de recherche interactions plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), and Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2012

29. Study of expression heterogeneity of flagellar genes in an insect pathogenic bacterium Xenorhabdus using single cell technologies

Author

Lanois, Anne, Jubelin, Grégory, Pages, Sylvie, Givaudan, Alain, Diversité, Génomes et Interactions Microorganismes-Insectes, Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Unité de Microbiologie (MIC), and Institut National de la Recherche Agronomique (INRA)

Subjects
Biodiversity and Ecology, Biodiversité et Ecologie, xenorhabdus nematophilus, spodoptera, lutte biologique, fluorescence, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, expression du génome, ComputingMilieux_MISCELLANEOUS, biodiversité
Abstract

National audience

Published
2011

30. La fluorescence : Solution pour exploiter les résultats d'expression de gènes bactériens dans une larve d'insecte infectée

Author

Pages, Sylvie, Lanois, Anne, Ginibre, Nadege, Diversité, Génomes et Interactions Microorganismes-Insectes, and Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)

Subjects
GFP - Green Fluorescent Protein, bactéries entomopathogènes, fluorescence, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, larves d’insectes, ComputingMilieux_MISCELLANEOUS, Xenorhabdus
Abstract

National audience

Published
2011

32. Unités de plasticité dans le génome de Photorhabdus : des réarrangements chromosomiques à l’échelle du temps du laboratoire à l’évolution des espèces

Author

Ogier, Jean-Claude, Calteau, Alexandra, Lanois, Anne, Pages, Sylvie, Roche, David, Rouy, Zoé, Teyssier, Corinne, Tailliez, Patrick, Medigue, Claudine, Jumas-Bilak, Estelle, Givaudan, Alain, Gaudriault, Sophie, Diversité, Génomes et Interactions Microorganismes-Insectes, Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Département Microbiologie et Chaîne Alimentaire (MICA), Institut National de la Recherche Agronomique (INRA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Montpellier 1 (UM1), and Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2010

34. The entomopathogenic bacterial endosymbionts Xenorhabdus and Photorhabdus: convergent lifestyles from divergent genomes

Author

Chaston, John M., Suen, Garret, Tucker, Sarah L., Andersen, Aaron W., Bhasin, Archna, Bode, Edna, Bode, Helge Björn, Brachmann, Alexander Oliver, Cowles, Charles E., Cowles, Kimberly N., Darby, Creg, Léon, Limaris de, Drace, Kevin, Du, Zijin, Givaudan, Alain, Tran, Erin E. Herbert, Jewell, Kelsea A., Knack, Jennifer J., Krasomil-Osterfeld, Karina C., Kukor, Ryan, Lanois, Anne, Latreille, Phil, Leimgruber, Nancy K., Lipke, Carolyn M., Liu, Renyi, Lu, Xiaojun, Martens, Eric C., Marri, Pradeep R., Médigue, Claudine, Menard, Megan L., Miller, Nancy M., Morales-Soto, Nydia, Norton, Stacie, Ogier, Jean-Claude, Orchard, Samantha S., Park, Dongjin, Park, Youngjin, Qurollo, Barbara A., Renneckar Sugar, Darby, Richards, Gregory R., Rouy, Zoé, Slominski, Brad, Slominski, Kathryn, Snyder, Holly, Tjaden, Brian C., Hoeven, Ransome van der, Welch, Roy D., Wheeler, Cathy, Xiang, Bosong, Barbazuk, Brad, Gaudriault, Sophie, Goodner, Brad, Slater, Steven C., Forst, Steven, Goldman, Barry S., Goodrich-Blair, Heidi, Chaston, John M., Suen, Garret, Tucker, Sarah L., Andersen, Aaron W., Bhasin, Archna, Bode, Edna, Bode, Helge Björn, Brachmann, Alexander Oliver, Cowles, Charles E., Cowles, Kimberly N., Darby, Creg, Léon, Limaris de, Drace, Kevin, Du, Zijin, Givaudan, Alain, Tran, Erin E. Herbert, Jewell, Kelsea A., Knack, Jennifer J., Krasomil-Osterfeld, Karina C., Kukor, Ryan, Lanois, Anne, Latreille, Phil, Leimgruber, Nancy K., Lipke, Carolyn M., Liu, Renyi, Lu, Xiaojun, Martens, Eric C., Marri, Pradeep R., Médigue, Claudine, Menard, Megan L., Miller, Nancy M., Morales-Soto, Nydia, Norton, Stacie, Ogier, Jean-Claude, Orchard, Samantha S., Park, Dongjin, Park, Youngjin, Qurollo, Barbara A., Renneckar Sugar, Darby, Richards, Gregory R., Rouy, Zoé, Slominski, Brad, Slominski, Kathryn, Snyder, Holly, Tjaden, Brian C., Hoeven, Ransome van der, Welch, Roy D., Wheeler, Cathy, Xiang, Bosong, Barbazuk, Brad, Gaudriault, Sophie, Goodner, Brad, Slater, Steven C., Forst, Steven, Goldman, Barry S., and Goodrich-Blair, Heidi

Abstract

Members of the genus Xenorhabdus are entomopathogenic bacteria that associate with nematodes. The nematode-bacteria pair infects and kills insects, with both partners contributing to insect pathogenesis and the bacteria providing nutrition to the nematode from available insect-derived nutrients. The nematode provides the bacteria with protection from predators, access to nutrients, and a mechanism of dispersal. Members of the bacterial genus Photorhabdus also associate with nematodes to kill insects, and both genera of bacteria provide similar services to their different nematode hosts through unique physiological and metabolic mechanisms. We posited that these differences would be reflected in their respective genomes. To test this, we sequenced to completion the genomes of Xenorhabdus nematophila ATCC 19061 and Xenorhabdus bovienii SS-2004. As expected, both Xenorhabdus genomes encode many anti-insecticidal compounds, commensurate with their entomopathogenic lifestyle. Despite the similarities in lifestyle between Xenorhabdus and Photorhabdus bacteria, a comparative analysis of the Xenorhabdus, Photorhabdus luminescens, and P. asymbiotica genomes suggests genomic divergence. These findings indicate that evolutionary changes shaped by symbiotic interactions can follow different routes to achieve similar end points.

Published
2011

38. The Entomopathogenic Bacterial Endosymbionts Xenorhabdus and Photorhabdus: Convergent Lifestyles from Divergent Genomes

Author

Chaston, John M., primary, Suen, Garret, additional, Tucker, Sarah L., additional, Andersen, Aaron W., additional, Bhasin, Archna, additional, Bode, Edna, additional, Bode, Helge B., additional, Brachmann, Alexander O., additional, Cowles, Charles E., additional, Cowles, Kimberly N., additional, Darby, Creg, additional, de Léon, Limaris, additional, Drace, Kevin, additional, Du, Zijin, additional, Givaudan, Alain, additional, Herbert Tran, Erin E., additional, Jewell, Kelsea A., additional, Knack, Jennifer J., additional, Krasomil-Osterfeld, Karina C., additional, Kukor, Ryan, additional, Lanois, Anne, additional, Latreille, Phil, additional, Leimgruber, Nancy K., additional, Lipke, Carolyn M., additional, Liu, Renyi, additional, Lu, Xiaojun, additional, Martens, Eric C., additional, Marri, Pradeep R., additional, Médigue, Claudine, additional, Menard, Megan L., additional, Miller, Nancy M., additional, Morales-Soto, Nydia, additional, Norton, Stacie, additional, Ogier, Jean-Claude, additional, Orchard, Samantha S., additional, Park, Dongjin, additional, Park, Youngjin, additional, Qurollo, Barbara A., additional, Sugar, Darby Renneckar, additional, Richards, Gregory R., additional, Rouy, Zoé, additional, Slominski, Brad, additional, Slominski, Kathryn, additional, Snyder, Holly, additional, Tjaden, Brian C., additional, van der Hoeven, Ransome, additional, Welch, Roy D., additional, Wheeler, Cathy, additional, Xiang, Bosong, additional, Barbazuk, Brad, additional, Gaudriault, Sophie, additional, Goodner, Brad, additional, Slater, Steven C., additional, Forst, Steven, additional, Goldman, Barry S., additional, and Goodrich-Blair, Heidi, additional

Published
2011
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40. The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens

Author

Duchaud, Eric, primary, Rusniok, Christophe, additional, Frangeul, Lionel, additional, Buchrieser, Carmen, additional, Givaudan, Alain, additional, Taourit, Séad, additional, Bocs, Stéphanie, additional, Boursaux-Eude, Caroline, additional, Chandler, Michael, additional, Charles, Jean-François, additional, Dassa, Elie, additional, Derose, Richard, additional, Derzelle, Sylviane, additional, Freyssinet, Georges, additional, Gaudriault, Sophie, additional, Médigue, Claudine, additional, Lanois, Anne, additional, Powell, Kerrie, additional, Siguier, Patricia, additional, Vincent, Rachel, additional, Wingate, Vincent, additional, Zouine, Mohamed, additional, Glaser, Philippe, additional, Boemare, Noël, additional, Danchin, Antoine, additional, and Kunst, Frank, additional

Published
2003
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45. FliZ Is a Global Regulatory Protein Affecting the Expression of Flagellar and Virulence Genes in Individual Xenorhabdus nematophila Bacterial Cells.

Author

Jubelin, Grégory, Lanois, Anne, Severac, Dany, Rialle, Stéphanie, Longin, Cyrille, Gaudriault, Sophie, and Givaudan, Alain

Subjects
*DNA-binding proteins, *XENORHABDUS nematophilus, *GENE expression in bacteria, *VIRULENCE of bacteria, *BACTERIAL cells
Abstract

Heterogeneity in the expression of various bacterial genes has been shown to result in the presence of individuals with different phenotypes within clonal bacterial populations. The genes specifying motility and flagellar functions are coordinately regulated and form a complex regulon, the flagellar regulon. Complex interplay has recently been demonstrated in the regulation of flagellar and virulence gene expression in many bacterial pathogens. We show here that FliZ, a DNA-binding protein, plays a key role in the insect pathogen, Xenorhabdus nematophila, affecting not only hemolysin production and virulence in insects, but efficient swimming motility. RNA-Seq analysis identified FliZ as a global regulatory protein controlling the expression of 278 Xenorhabdus genes either directly or indirectly. FliZ is required for the efficient expression of all flagellar genes, probably through its positive feedback loop, which controls expression of the flhDC operon, the master regulator of the flagellar circuit. FliZ also up- or downregulates the expression of numerous genes encoding non-flagellar proteins potentially involved in key steps of the Xenorhabdus lifecycle. Single-cell analysis revealed the bimodal expression of six identified markers of the FliZ regulon during exponential growth of the bacterial population. In addition, a combination of fluorescence-activated cell sorting and RT-qPCR quantification showed that this bimodality generated a mixed population of cells either expressing (“ON state”) or not expressing (“OFF state”) FliZ-dependent genes. Moreover, studies of a bacterial population exposed to a graded series of FliZ concentrations showed that FliZ functioned as a rheostat, controlling the rate of transition between the “OFF” and “ON” states in individuals. FliZ thus plays a key role in cell fate decisions, by transiently creating individuals with different potentials for motility and host interactions. [ABSTRACT FROM AUTHOR]

Published
2013
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46. Gnotobiological Study of Infective Juveniles and Symbionts of Steinernema scapterisci:A Model to Clarify the Concept of the Natural Occurrence of Monoxenic Associations in Entomopathogenic Nematodes

Author

Bonifassi, Eliane, Fischer-Le Saux, Marion, Boemare, Noël, Lanois, Anne, Laumond, Christian, and Smart, Grover

Abstract

Gnotobiology of Steinernema scapterisciand bacteriological study of its symbiont confirmed that this nematode harbors a symbiotic species of Xenorhabdus,as do other Steinermenaspecies. Based on phenotypic and 16S rDNA data, this Xenorhabdusstrain UY61 could be distinguished from other Xenorhabdusspecies. Bacteria reported previously as being associated with this nematode and belonging to several other genera were probably contaminating bacteria located in the intercuticular space of the infective juveniles (IJs). These bacteria were detrimental to nematode reproduction in Galleria mellonella.Axenic S. scapterisciand its symbiont Xenorhabdusstrain UY61 alone were not pathogenic to G. mellonella.The combination of both partners reestablished the pathogenicity of the complex toward G. mellonella.This combination also gave the best yields of IJs when produced in this insect and in vitroproduction on artificial diet.

Published
1999
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47. OxyR is required for oxidative stress resistance of the entomopathogenic bacterium Xenorhabdus nematophila and has a minor role during the bacterial interaction with its hosts.

Author

Bientz V, Lanois A, Ginibre N, Pagès S, Ogier JC, George S, Rialle S, and Brillard J

Subjects
Animals, Rhabditida microbiology, Rhabditida genetics, Rhabditida physiology, Larva microbiology, Virulence, Regulon, Gene Expression Profiling, Mutation, Xenorhabdus genetics, Xenorhabdus metabolism, Xenorhabdus physiology, Oxidative Stress, Bacterial Proteins genetics, Bacterial Proteins metabolism, Gene Expression Regulation, Bacterial, Symbiosis
Abstract

Xenorhabdus nematophila is a Gram-negative bacterium, mutualistically associated with the soil nematode Steinernema carpocapsae , and this nemato-bacterial complex is parasitic for a broad spectrum of insects. The transcriptional regulator OxyR is widely conserved in bacteria and activates the transcription of a set of genes that influence cellular defence against oxidative stress. It is also involved in the virulence of several bacterial pathogens. The aim of this study was to identify the X. nematophila OxyR regulon and investigate its role in the bacterial life cycle. An oxyR mutant was constructed in X. nematophila and phenotypically characterized in vitro and in vivo after reassociation with its nematode partner. OxyR plays a major role during the X. nematophila resistance to oxidative stress in vitro . Transcriptome analysis allowed the identification of 59 genes differentially regulated in the oxyR mutant compared to the parental strain. In vivo , the oxyR mutant was able to reassociate with the nematode as efficiently as the control strain. These nemato-bacterial complexes harbouring the oxyR mutant symbiont were able to rapidly kill the insect larvae in less than 48 h after infestation, suggesting that factors other than OxyR could also allow X. nematophila to cope with oxidative stress encountered during this phase of infection in insect. The significantly increased number of offspring of the nemato-bacterial complex when reassociated with the X. nematophila oxyR mutant compared to the control strain revealed a potential role of OxyR during this symbiotic stage of the bacterial life cycle.

Published
2024
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48. Missense Mutations in the CrrB Protein Mediate Odilorhabdin Derivative Resistance in Klebsiella pneumoniae .

Author

Pantel L, Juarez P, Serri M, Boucinha L, Lessoud E, Lanois A, Givaudan A, Racine E, and Gualtieri M

Abstract

NOSO-502 is a preclinical antibiotic candidate of the Odilorhabdin class. This compound exhibits activity against Enterobacteriaceae pathogens, including carbapenemase-producing bacteria and most of the Colistin (CST)-resistant strains. Among a collection of CST-resistant Klebsiella pneumoniae strains harboring mutations on genes pmrAB , mgrB , phoPQ , and crrB , only those bearing mutations in gene crrB were found to be resistant to NOSO-502.CrrB is a histidine kinase which acts with the response regulator CrrA to modulate the PmrAB system, which finally induces the restructuring of the lipopolysaccharide present on the outer membrane and thus leading to CST resistance. Moreover, crrB mutations also enhance the transcription of neighboring genes such as H239_3063, an ABC transporter transmembrane region; H239_3064, a putative efflux pump also known as KexD; and H239_3065, a N -acetyltransferase.To elucidate the mechanism of resistance to NOSO-502 induced by CrrB missense mutations in K. pneumoniae , mutants of NCTC 13442 and ATCC BAA-2146 strains resistant to NOSO-502 and CST with single amino acid substitutions in CrrB (S8N, F33Y, Y34N, W140R, N141I, P151A, P151L, P151S, P151T, F303Y) were selected. Full susceptibility to NOSO-502 was restored in crrA or crrB deleted K. pneumoniae NCTC 13442 CrrB(P151L) mutants, confirming the role of CrrAB in controlling this resistance pathway. Deletion of kexD (but no other neighboring genes) in the same mutant also restored NOSO-502-susceptibility. Upregulation of the kexD gene expression was observed for all CrrB mutants. Finally, plasmid expression of kexD in a K. pneumoniae strain missing the locus crrABC and kexD significantly increased resistance to NOSO-502., (Copyright © 2021 American Society for Microbiology.)

Published
2023
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