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1. 5-HT4 receptors constitutively promote the non-amyloidogenic pathway of APP cleavage and interact with ADAM10

Author: Maud Cochet, Philippe Marin, Aline Dumuis, Joël Bockaert, Stefan F. Lichtenthaler, Sylvie Claeysen, Elisabeth Cassier, Florence Gaven, Romain Donneger, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), German Research Center for Neurodegenerative Diseases - Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Supported by grants from CNRS, INSERM, the French Minister of Research (ANR Blanc 2006, to AD and SC, ANR Blanc 2012 'ADAMGUARD' to J.B.), the 'Fondation pour la Recherche Médicale' (FRM, to P.M.), the France Alzheimer association (to S.C.), the Soroptimist International (French Union, to S.C.), the Languedoc Roussillon region ('Chercheuse d’Avenir' 2012, to S.C.) and the competence network degenerative dementias (BMBF-KNDD, to S.F.L.). M.C. was a recipient of fellowships from the CNRS, the Languedoc Roussillon region and FRM. E.C. was supported by ANR Blanc 2006., ANR-06-BLAN-0087,GPCR dimers,Molecular and dynamic aspects of G-protein coupled receptor dimers functioning(2006), and ANR-12-BSV4-0008,ADAMGUARD,Les réseaux protéiques associés aux récepteurs 5 HT4 : gardes rapprochées du trafic de l'ADAM10 et de l'APP(2012)
Subjects: Physiology, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, etiology [Alzheimer Disease], ADAM10, Stimulation, Biochemistry, Receptors, G-Protein-Coupled, cytology [Cerebral Cortex], ADAM10 Protein, Amyloid beta-Protein Precursor, 0302 clinical medicine, metabolism [Amyloid beta-Protein Precursor], Amyloid precursor protein, Cyclic AMP, metabolism [Receptors, Serotonin, 5-HT4], physiology [Receptors, Serotonin, 5-HT4], Receptor, Cerebral Cortex, 0303 health sciences, General Medicine, Alzheimer's disease, pharmacology [Benzofurans], serotonin, Neuroprotective Agents, Alpha secretase, ddc:540, metabolism [Cyclic AMP], Agonist, medicine.drug_class, ADAM10 protein, human, Cognitive Neuroscience, physiology [Receptors, G-Protein-Coupled], Biology, Neuroprotection, 03 medical and health sciences, Serotonin 5-HT4 Receptor Agonists, Alzheimer Disease, mental disorders, medicine, Humans, physiology [Amyloid Precursor Protein Secretases], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], 030304 developmental biology, G protein-coupled receptor, Benzofurans, pharmacology [Neuroprotective Agents], Membrane Proteins, Cell Biology, metabolism [Amyloid Precursor Protein Secretases], ADAM Proteins, HEK293 Cells, metabolism [ADAM Proteins], biology.protein, Alpha-secretase, Receptors, Serotonin, 5-HT4, sAPP alpha, prucalopride, Amyloid Precursor Protein Secretases, metabolism [Membrane Proteins], pharmacology [Serotonin 5-HT4 Receptor Agonists], 030217 neurology & neurosurgery
Abstract: International audience; In addition to the amyloidogenic pathway, amyloid precursor protein (APP) can be cleaved by α-secretases, producing soluble and neuroprotective APP alpha (sAPPα) (nonamyloidogenic pathway) and thus preventing the generation of pathogenic amyloid-β. However, the mechanisms regulating APP cleavage by α-secretases remain poorly understood. Here, we showed that expression of serotonin type 4 receptors (5-HT(4)Rs) constitutively (without agonist stimulation) induced APP cleavage by the α-secretase ADAM10 and the release of neuroprotective sAPPα in HEK-293 cells and cortical neurons. This effect was independent of cAMP production. Interestingly, we demonstrated that 5-HT(4) receptors physically interacted with the mature form of ADAM10. Stimulation of 5-HT(4) receptors by an agonist further increased sAPPα secretion, and this effect was mediated by cAMP/Epac signaling. These findings describe a new mechanism whereby a GPCR constitutively stimulates the cleavage of APP by α-secretase and promotes the nonamyloidogenic pathway of APP processing.
Published: 2012
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2. Rapid and transient evolution of local adaptation to seasonal host fruits in an invasive pest fly

Author: Laure Olazcuaga, Julien Foucaud, Candice Deschamps, Anne Loiseau, Jean-Loup Claret, Romain Vedovato, Robin Guilhot, Cyril Sévely, Mathieu Gautier, Ruth A. Hufbauer, Nicolas O. Rode, Arnaud Estoup, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Colorado State University [Fort Collins] (CSU), Department of Agricultural Biology, Colorado State University, Chambre d'Agriculture de l'Hérault (CA 34), Graduate Degree Program in Ecology, LO, MG, JF, and AE were supported by the Languedoc-Roussillon region (France) through the European Union program FEDER FSE IEJ 2014-2020 (project CPADROL) and the INRAE scientific department SPE (AAP-SPE 2016). RAH acknowledges support from the National Science Foundation (DEB-0949619), USA Agriculture and Food Research Initiative award (2014-67013-21594), Hatch project 1012868, the French Agropolis Fondation (LabEx Agro-Montpellier) through the AAP 'International Mobility' (CfP 2015-02), the French programme investissement d'avenir, and the LabEx CEMEB through the AAP 'invited scientist 2016.' NOR acknowledges support from the CeMEB LabEx/University of Montpellier (ANR-10-LABX-0004) and the INRAE scientific department SPE (AAP-SPE 2021PestAdapt)., and ANR-10-LABX-0004,CeMEB,Mediterranean Center for Environment and Biodiversity(2010)
Subjects: Adaptive phenotypic plasticity, [SDV]Life Sciences [q-bio], Drosophila suzukii, Genetics, genetic effects, reciprocal common garden experiment, Ecology, Evolution, Behavior and Systematics, host fruits, local adaptation
Abstract: Both local adaptation and adaptive phenotypic plasticity can influence the match between phenotypic traits and local environmental conditions. Theory predicts that environments stable for multiple generations promote local adaptation, while highly heterogeneous environments favor adaptive phenotypic plasticity. However, when environments have periods of stability mixed with heterogeneity, the relative importance of local adaptation and adaptive phenotypic plasticity is unclear. Here, we used Drosophila suzukii as a model system to evaluate the relative influence of genetic and plastic effects on the match of populations to environments with periods of stability from three to four generations. This invasive pest insect can develop within different fruits, and persists throughout the year in a given location on a succession of distinct host fruits, each one being available for only a few generations. Using reciprocal common environment experiments of natural D. suzukii populations collected from cherry, strawberry and blackberry, we found that both oviposition preference and offspring performance were higher on medium made with the fruit from which the population originated, than on media made with alternative fruits. This pattern, which remained after two generations in the laboratory, was analyzed using a statistical method we developed to quantify the contributions of local adaptation and adaptive plasticity in determining fitness. Altogether, we found that genetic effects (local adaptation) dominate over plastic effects (adaptive phenotypic plasticity). Our study demonstrates that spatially and temporally variable selection does not prevent the rapid evolution of local adaptation in natural populations. The speed and strength of adaptation may be facilitated by several mechanisms including a large effective population size and strong selective pressures imposed by host plants.Impact SummaryNatural populations often exhibit good “fit” to the environment they are in. However, environments change over time and space, and following change, the fit between a population and its environment may be poor. A question of long-standing interest is how do populations track changing environments to maintain fitness? Two main mechanisms are known: (i) genetic shifts in the form of local adaptation, in which traits evolve over time through differences in fitness of individuals harboring different genetic variants, and (ii) plastic shifts, or adaptive phenotypic plasticity, in which traits immediately change in response to environmental change. Adaptation is common when environments change over multiple generations, while plasticity is common when environments change over an individual’s lifetime. However, it remains unclear whether plasticity or adaptation is more vital to maintaining fitness when environments change at an intermediate pace.Drosophila suzukii is well-suited to evaluating the relative importance of plasticity and adaptation in response to an intermediate pace of environmental change. This invasive pest species experiences an environment that shifts every 1-4 generations as host fruits change over time and space. Here, we studied natural populations of D. suzukii collected from different hosts. Using reciprocal common environment experiments, we evaluated their fitness on their source and alternative hosts.Drosophila suzukii populations were most fit on their source host, successfully tracking an intermediate pace of environmental change. We developed a statistical method to quantify the contributions of adaptive plasticity and local adaptation in determining fitness. We found that fitness was mainly maintained through local adaptation to each new host in succession. This study highlights that spatially and temporally variable selection does not prevent local adaptation and, on the contrary, illustrates how rapid the adaptive process can be. It also provides a novel statistical tool that can be applied to other systems
Published: 2022
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3. Low effective population sizes in Amblyomma variegatum, the tropical bont tick

Author: Huber, Karine, Jacquet, S., Rivallan, R., Adakal, H., Vachiery, N., Risterucci, A.M., Chevillon, Christine, Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Evolution of host-microbe communities (MIVEGEC-EVCO), Processus Écologiques et Évolutifs au sein des Communautés (PEEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Transversal project AAP INRA-CIRAD 'Dynamic and genetic diversity of tick populations and transmitted pathogens', Languedoc-Roussillon region, France, Ministere de l'Agriculture et de l'Alimentation, Centre de Cooperation Internationale en Recherche Agronomiquepour le Developpement (CIRAD), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Subjects: Male, Population Density, Polymorphism, Genetic, Microsatellite markers, Ixodidae, Amblyomma variegatum, L73 - Maladies des animaux, L10 - Génétique et amélioration des animaux, Écologie des populations, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Effective population size, Burkina Faso, parasitic diseases, Animals, Female, Marqueur génétique, Population animale, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Microsatellite Repeats
Abstract: International audience; Effective population sizes have rarely been estimated in ticks despite the importance of this parameter for evaluating the evolutionary and adaptive potential of tick populations. The present study was aimed at evaluating the effective population sizes of Amblyomma variegatum, the tropical bont tick, in three villages in Burkina Faso. For this purpose, microsatellites markers were developed. Eight out of 19 assessed markers provided good amplification results with 4 to 24 alleles recorded per marker on 216 genotyped ticks. The within-samples polymorphism was congruent with Hardy-Weinberg expectations at four markers while sex linkage and/or null alleles were observed at the others. As sampling involved two tick generations, effective population sizes were independently estimated by two methods insensitive to heterozygosity: the first one is based on linkage dis-equilibrium analysis within a single cohort while the second uses the changes in allele frequencies across generations. Both methods estimated the number of reproducing ticks ranging from two to a few tens reproductive adults per village and cohort. Such small estimates are congruent with the rarity of records of acaricide resistance in A. variegatum.
Published: 2019
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4. Adapting gravimetric sorption analyzer to estimate water vapor diffusivity in micrometric size cellulose particles

Author: Sébastien Gaucel, Valentin Thoury-Monbrun, Hélène Angellier-Coussy, Nathalie Gontard, Valérie Guillard, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), MALICE project (call 'Chercheur d’avenir 2015'), which is co-financed by the European Regional Development Fund (FEDER), and Languedoc-Roussillon region
Subjects: vapeur d'eau, micrométrie, Materials science, Polymers and Plastics, Population, Analytical chemistry, 02 engineering and technology, 010402 general chemistry, Thermal diffusivity, 01 natural sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, courbe de sorption, Sample preparation, education, Diffusion coefficient, Water vapor, education.field_of_study, Sorption, Quartz crystal microbalance, coefficient de diffusion, 021001 nanoscience & nanotechnology, micrometry, cellulose, 0104 chemical sciences, Gravimetric analysis, Dynamic vapor sorption, 0210 nano-technology, Vegetal particle
Abstract: This study aims at developing a reliable and simple-to-implement methodology for the estimation of apparent water vapor diffusivity at the scale of micrometric size cellulose particles based on gravimetric measurements. The water vapor apparent diffusivity value was evaluated by using a quartz crystal microbalance (QCM) on a cellulose sample of 1 µg was 4 × 10−12 m2 s−1. Water vapor sorption kinetics at successive relative humidity (RH) steps were measured using a dynamic vapor sorption (DVS) microbalance by testing two types of sample preparation, i.e. either a powder bed or a compressed tablet. Water vapor apparent diffusivity was identified at each RH step by employing an analytic solution corresponding to the sample type: plane sheet for both powder bed and tablet considered as porous media and finite cylinder for a population of particles. The impact of the initial cellulose sample mass was also investigated. Water vapor diffusivity values varied from 6 × 10−15 to 4 × 10−10 m2 s−1 depending on the sample mass, sample preparation mode, analytic solution and/or sample porosity. The DVS-based methodology was compared to the value obtained from QCM measurements.
Published: 2019
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5. Assessing the potential of quartz crystal microbalance to estimate water vapor transfer in micrometric size cellulose particles

Author: Valérie Guillard, Vincent Rouessac, Sébastien Gaucel, Hélène Angellier-Coussy, Valentin Thoury-Monbrun, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Génie et Microbiologie des Procédés Alimentaires (GMPA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut Européen des membranes (IEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM), Dauphine Recherches en Management (DRM), Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), MALICE project (call 'Chercheur d’avenir 2015'), European Regional Development Fund (FEDER), Languedoc-Roussillon region, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université Paris Dauphine-PSL
Subjects: Morphological analysis, vapeur d'eau, Materials science, Polymers and Plastics, Quartz Crystal Microbalance, particule végétale, Analytical chemistry, Ingénierie des aliments, 02 engineering and technology, 010402 general chemistry, Thermal diffusivity, 01 natural sciences, Adsorption, microbalance à quartz, Materials Chemistry, Food engineering, [CHIM]Chemical Sciences, Relative humidity, Diffusion coefficient, ComputingMilieux_MISCELLANEOUS, Water vapor, Vegetal particle, Sorption, analyse morphologique, Organic Chemistry, Quartz crystal microbalance, coefficient de diffusion, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Particle, Particle size, 0210 nano-technology, Dispersion (chemistry)
Abstract: International audience; This study aims at assessing the use of a quartz crystal microbalance (QCM) coupled with an adsorption system to measure water vapor transfer properties in micrometric size cellulose particles. This apparatus allows measuring successfully water vapor sorption kinetics at successive relative humidity (RH) steps on a dispersion of individual micrometric size cellulose particles (1 μg) with a total acquisition duration of the order of one hour. Apparent diffusivity and water uptake at equilibrium were estimated at each step of RH by considering two different particle geometries in mass transfer modeling, i.e. sphere or finite cylinder, based on the results obtained from image analysis. Water vapor diffusivity values varied from 2.4 × 10−14 m2.s−1 to 4.2 × 10−12 m2.s−1 over the tested RH range (0 to 80%) whatever the model used. A finite cylinder or spherical geometry could be used equally for diffusivity identification for a particle size aspect ratio lower than 2.
Published: 2018
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6. Dry fractionation of olive pomace as a sustainable process to produce fillers for biocomposites

Author: Sarah Lammi, Hélène Angellier-Coussy, Claire Mayer-Laigle, Djamel Djenane, Abdellatif Barakat, Nathalie Gontard, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), PNE Program (2016–2017, grant number 396 assigned on June 9th, 2016) of the Algerian Ministry of Higher Education and Scientific Research, MALICE project (call 'Chercheur d'avenir 2015'), which is co-financed by the European Regional Development Fund (FEDER), Languedoc-Roussillon region (grant number 2015-005910)., and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Subjects: 020209 energy, General Chemical Engineering, Characterization, Ingénierie des aliments, 02 engineering and technology, Fractionation, fractionnement, engineering.material, broyage, olive, grignon, chemistry.chemical_compound, Olive pomace, [SDV.IDA]Life Sciences [q-bio]/Food engineering, 0202 electrical engineering, electronic engineering, information engineering, écoprocédé, Food engineering, Olive oil extraction, Lignin, fractionation, Cellulose, Effluent, Ball mill, Dry fractionation, Grinding, Sorting, déchet agroindustriel, caractérisation physico chimique, Pulp (paper), Pomace, Pulp and paper industry, grinding, chemistry, biomatériau, engineering
Abstract: Olive pomace (OP) is the agro-industrial residue of olive oil extraction composed of residual pulp and stone. This work aims at exploring the possibility of using dry fractionation (combination of grinding and sorting processes) to produce pulp-rich and stone-rich fractions with the highest purity and yield. The physical-chemical characteristics (composition, thermal stability, color, surface free energy) of the obtained powders were discussed in relation to the applied processes. It was shown that dry fractionation could be successfully used to convert OP into valuable fractions using processes avoiding the consumption of water and the generation of effluents or co-products. Results revealed that the separation of the pulp from the stone using friction solicitations in a ball mill operating in mild conditions (2 min at a frequency of 15 Hz) was as efficient as wet fractionation in terms of powder characteristics, achieving a total yield of 99.4% against only 82.1% in the case of wet fractionation and without using water while a water:biomass ratio of 5:1 was required for wet fractionation. Produced powders exhibited contrasted biochemical composition (either rich in lignin or cellulose) and surface free energy, and were thermally stable up to at least 210 °C. It was concluded that they could be interestingly used as raw resources for the production of fillers that will be further incorporated in polymer matrices to produce a range of biocomposites.
Published: 2018
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7. Effect of storage in glass and polyethylene terephthalate bottles on the sensory characteristics of rosé wine

Author: Caille, Soline, Salmon, Jean-Michel, Samson, Alain, Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Unité expérimentale de Pech-Rouge, Institut National de la Recherche Agronomique (INRA), OSEO (French State Agency for Innovation, Herault, France), FEDER (Fonds Europeen de Developpement Regional), Languedoc Roussillon Region, Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Unité expérimentale de Pech-Rouge (PECH ROUGE)
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, shelf-life, rose wine, caractérisation sensorielle, polyethylene terephthalate (PET) bottle, digestive, oral, and skin physiology, glass bottle, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, vin rose, wine, sensory analysis
Abstract: Background and Aims: We studied the ageing of rosé wine in polyethylene terephthalate (PET) bottles compared to that in the reference glass bottle, as the PET bottle needs less energy for manufacture, transport and recycling, which is of benefit for minimising environmental concerns. Our study focused on the evolution of the sensory characteristics of rosé wine.Methods and Results: Sensory characteristics were monitored over a period that corresponded to the duration of marketing of this type of wine. Several types of PET bottle – recycled, not recycled, with or without oxygen scavengers – were compared with the glass bottle. The wines were subjected to sensory analysis using the methodology of conventional descriptive profile by an expert panel. The impact of PET bottles on the colour and sensory characteristics of rosé wine was significant, which was due mainly to oxygen transfer through the PET bottle. The impact was much less for the taste characteristics.Conclusions: Packaging rosé wine in PET bottles has a significant impact on the evolution of rosé wine.Significance of the Study: These results can guide professionals in the choice of packaging according to the wine.
Published: 2018
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8. APOE ε4 and risk for Alzheimer's disease: Do regionally distributed white matter hyperintensities play a role?

Author: Jennifer J. Manly, Sylvaine Artero, Qolamreza R. Razlighi, Florence Portet, Richard Mayeux, José A. Luchsinger, Nicole Schupf, Adam M. Brickman, Atul Narkhede, Tasnime N. Akbaraly, Frank A. Provenzano, Yaakov Stern, Karen Ritchie, Lyndsey E. Collins-Praino, Taub Institute for Research on Alzheimer's Disease and the Aging Brain [New-York, NY, USA] (College of Physicians & Surgeons), Columbia University [New York], G.H. Sergievsky Center [New-York, NY, USA] (College of Physicians and Surgeons), Department of Neurology [New York, NY, USA], Department of Epidemiology [New York, NY, États-Unis], Mailman School of Public Health [New York, NY, États-Unis], Columbia University [New York]-Columbia University [New York], Department of Psychiatry [New York, NY, USA], Department of Medicine [New-York, NY, USA] (College of Physicians and Surgeons), Neuropsychiatrie : recherche épidémiologique et clinique (PSNREC), Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Epidemiology and Public Health, University College of London [London] (UCL), This work was supported in part by grants from National In-stitutes of Health (AG037212, AG007232, AG029949, andAG034189), regional government of Languedoc-Roussillon(http://www.laregion.fr), the Agence Nationale de la Re-cherche (ANR:http://www.agence-nationale-recherche.fr),and an unconditional grant from Novartis (http://www.novartis.fr). Additional support by France Alzheimer(http://www.francealzheimer.org/). TNA is supported bythe Languedoc-Roussillon Region (Chercheur d’avenirGrant 2011)., Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), and Artero, Sylvaine
Subjects: Male, Apolipoprotein E, Pathology, Epidemiology, Apolipoprotein E4, [SDV.MHEP.PSM] Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, Disease, Neuropsychological Tests, 0302 clinical medicine, Parietal Lobe, Image Processing, Computer-Assisted, Longitudinal Studies, Aged, 80 and over, Neurologic Examination, 0303 health sciences, Health Policy, Parietal lobe, white matter hyperintensities, Magnetic Resonance Imaging, White Matter, 3. Good health, Psychiatry and Mental health, medicine.anatomical_structure, Female, Cerebral amyloid angiopathy, Alzheimer disease, Alzheimer's disease, Psychology, APOE, medicine.medical_specialty, Genotype, behavioral disciplines and activities, Article, White matter, 03 medical and health sciences, Cellular and Molecular Neuroscience, Developmental Neuroscience, mental disorders, medicine, Humans, Dementia, Aged, Retrospective Studies, 030304 developmental biology, Analysis of Variance, medicine.disease, Hyperintensity, [SDV.MHEP.PSM]Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, Linear Models, Neurology (clinical), Geriatrics and Gerontology, 030217 neurology & neurosurgery
Abstract: Background We previously demonstrated that parietal lobe white matter hyperintensities (WMH) increase the risk for Alzheimer's disease (AD). Here, we examined whether individuals with apolipoprotein E gene ( APOE e4) have increased parietal WMH volume. Methods Participants were from the Washington Heights-Inwood Columbia Aging Project (WHICAP; n = 694, 47 with dementia) in northern Manhattan and the Etude Sante Psychologique Prevalence Risques et Traitement study (ESPRIT; n = 539, 8 with dementia) in Montpellier. The association between regional WMH and APOE e4 was examined separately in each group and then in a combined analysis. Results In WHICAP, e4 carriers had higher WMH volume particularly in parietal and occipital lobes. In ESPRIT, e4 carriers had elevated WMH particularly in parietal and temporal lobes. In the combined analysis, e4 carriers had higher WMH in parietal and occipital lobes. Increased WMH volume was associated with increased frequency of dementia irrespective of APOE e4 status; those with the e4 were more likely to have dementia if they also had increased parietal WMH. Conclusions APOE e4 is associated with increased parietal lobe WMH.
Published: 2014
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9. Reduction of leaf area and symptom severity as proxies of disease-induced plant mortality: The example of the Cauliflower mosaic virus infecting two Brassicaceae hosts

Author: Sophie Leblaye, Yannis Michalakis, Rémy Froissart, Juliette Doumayrou, Biologie et Génétique des interactions Plantes-parasites pour la Protection Intégrée, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Université Montpellier 1 (UM1), Evolution Théorique et Expérimentale (MIVEGEC-ETE), Perturbations, Evolution, Virulence (PEV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Emergent Infectious Disease Program of the Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA SPE), Languedoc-Roussillon region, CNRS, and Institut de Recherche pour le Developpement (IRD)
Subjects: 0106 biological sciences, Cancer Research, Veterinary medicine, Time Factors, [SDV]Life Sciences [q-bio], Arabidopsis, Virulence, Disease, 01 natural sciences, 03 medical and health sciences, Caulimovirus, Virology, Botany, Brassica rapa, Arabidopsis thaliana, Mortality proxies, Plant Diseases, 030304 developmental biology, Cauliflower mosaic virus, 0303 health sciences, biology, Host (biology), Mortality rate, Brassicarapa, fungi, food and beverages, Brassicaceae, biology.organism_classification, Survival Analysis, Plant Leaves, Infectious Diseases, Biomarkers, 010606 plant biology & botany
Abstract: BGPI : équipe 2; International audience; Disease induced effects on host survival are important to understand the evolution of parasitic virulence and host resistance/tolerance. Unfortunately, experiments evaluating such effects are in most cases logistically demanding justifying the measurement of survival proxies. For plant hosts commonly used proxies are leaf area and the nature and severity of visual qualitative disease symptoms. In this study we tested whether these traits are indeed correlated to the host mortality rate induced by viral infection. We infected Brassica rapa and Arabidopsis thaliana plants with different natural isolates of Cauliflower mosaic virus (CaMV) and estimated over time the development of symptoms and the relative reduction of leaf area compared to healthy plants and followed plant mortality. We observed that the mortality of infected plants was correlated with the relative reduction of leaf area of both B. rapa and A. thaliana. Measures of mortality were also correlated with the severity of visual qualitative symptoms but the magnitude of the correlations and the time frame at which they were significant depended on the host plant: stronger and earlier correlations were observed on A. thaliana.
Published: 2013
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10. Changing the soil surface management in vineyards: immediate and delayed effects on the growth and yield of grapevine

Author: Florian Celette, Aurélie Metay, Aude Ripoche, Christian Gary, Systèmes de Cultures Bananes, Ananas, et Plantains (Cirad-Es-UPR 26 Systèmes bananes et ananas), Département Environnements et Sociétés (Cirad-ES), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Fonctionnement et conduite des systèmes de culture tropicaux et méditerranéens (UMR SYSTEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre International de Hautes Etudes Agronomiques Méditerranéennes - Institut Agronomique Méditerranéen de Montpellier (CIHEAM-IAMM), Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), ISARA-LYON (ISARA-LYON), Agence Nationale de la Recherche-(ANR, French national research agency) ANR-05-PADD-010, INRA, Languedoc-Roussillon region (France), Systèmes de Cultures Bananes, Ananas, et Plantains (UPR 26 Systèmes bananes et ananas), Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre International de Hautes Etudes Agronomiques Méditerranéennes - Institut Agronomique Méditerranéen de Montpellier (CIHEAM-IAMM), Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and ISARA-Lyon
Subjects: 0106 biological sciences, sol, sol nu, développement végétal, Perennial plant, Vegetative reproduction, F08 - Systèmes et modes de culture, culture associée, F62 - Physiologie végétale - Croissance et développement, rendement, Plant Science, cropping system, 01 natural sciences, VEGETATIVE DEVELOPMENT, Soil management, Cropping system, SOIL SURFACE MANAGEMENT, système de culture, Cover crop, cultivated soil, 2. Zero hunger, biology, 04 agricultural and veterinary sciences, viticulture, Rendement des cultures, festuca, plant development, CROP MANAGEMENT, P33 - Chimie et physique du sol, vignoble, Festuca, Relation plante sol, Soil Science, vineyard, Vineyard, sol cultivé, soil, vitis vinifera, bare soil, fétuque, COVER CROP, PERENNIAL CROP, CULTURES PERENNES, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Croissance, 15. Life on land, biology.organism_classification, grapevine, yields catches, YIELD FORMATION, relay cropping, Agronomy, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, culture intercalaire, vigne, Cropping, 010606 plant biology & botany
Abstract: In a changing context, farmers adapt their cropping systems and, in this respect, the response time of the soil-crop system (changes in the soil resources and in the grapevine vegetative or reproductive development) has to be considered to maintain its agronomic performances. This is particularly true for perennial crops such as grapevine. This paper aims to analyse the short- and long-term effects of changes in soil surface management on grapevine performances. Avineyard in which bare soil inter-rows had been compared with a tall fescue intercrop since 2002 had each of these treatments split into two in 2007, with half of the intercrop being ploughed in and half of the bare soil being sown with fescue, resulting in four treatments whose effects were studied in 2008-2009. Grapevine growth, yield formation and grape quality were assessed. After intercrop destruction, grapevine vegetative growth was enhanced by the mineralization of organic matter from the first year, whereas after intercrop introduction, the competition for soil resources affected growth only in the second year. The grape yield depended on the number of bunches, elaborated the year before. One year after the change in soil surface management, the ranking of yield was as follows: permanent intercrop < intercrop destroyed < intercrop introduced < bare soil. The second year, this ranking altered (still in relation to the number of bunches) to: intercrop introduced < permanent intercrop < bare soil=intercrop destroyed. This study shows that the response times of the processes of grapevine vegetative growth and yield formation to a change in the cropping system vary from one to two years, and therefore, have to be considered carefully to manage the cropping system well.
Published: 2010
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11. Models based on ultraviolet spectroscopy, polyphenols, oligosaccharides and polysaccharides for prediction of wine astringency

Author: Pascale Williams, Thierry Doco, Alain Samson, Jean Marc Souquet, Jean-Claude Boulet, Soline Caille, Véronique Cheynier, Maris-Agnès Ducasse, Corinne Trarieux, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), UMT Qualinnov, Institut Français de la Vigne et du Vin (IFV), Unité expérimentale de Pech-Rouge (PECH ROUGE), Institut National de la Recherche Agronomique (INRA), This work was part of the Pilotype project which was made possible thanks to European, French and Languedoc-Roussillon region fundings., Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Unité expérimentale de Pech-Rouge, and Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Subjects: multiple linear regression, A230, Oligosaccharides, composé phénolique, astringency, sensory, Polysaccharide, Sensory analysis, Analytical Chemistry, sensory analysis, tannin, polyphénol, 0404 agricultural biotechnology, Polysaccharides, bovine serum albumine, [SDV.IDA]Life Sciences [q-bio]/Food engineering, vin, Tannin, oligosaccharide, Bovine serum albumin, wine, Wine, chemistry.chemical_classification, phenolic compound, analyse sensorielle, Chromatography, biology, tanin, Polyphenols, Taste Perception, Serum Albumin, Bovine, 04 agricultural and veterinary sciences, General Medicine, Oligosaccharide, 040401 food science, chemistry, oenologie, Polyphenol, polysaccharide, astringence, biology.protein, Spectrophotometry, Ultraviolet, Wine tasting, Tannins, Food Science
Abstract: Astringency elicited by tannins is usually assessed by tasting. Alternative methods involving tannin precipitation have been proposed, but they remain time-consuming. Our goal was to propose a faster method and investigate the links between wine composition and astringency. Red wines covering a wide range of astringency intensities, assessed by sensory analysis, were selected. Prediction models based on multiple linear regression (MLR) were built using UV spectrophotometry (190-400 nm) and chemical analysis (enological analysis, polyphenols, oligosaccharides and polysaccharides). Astringency intensity was strongly correlated (R(2) = 0.825) with tannin precipitation by bovine serum albumin (BSA). Wine absorbances at 230 nm (A230) proved more suitable for astringency prediction (R(2) = 0.705) than A280 (R(2) = 0.56) or tannin concentration estimated by phloroglucinolysis (R(2) = 0.59). Three variable models built with A230, oligosaccharides and polysaccharides presented high R(2) and low errors of cross-validation. These models confirmed that polysaccharides decrease astringency perception and indicated a positive relationship between oligosaccharides and astringency.
Published: 2016
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12. Contribution de l'imagerie infrarouge thermique infrarouge à haute résolution à un phénotypage sur site à haut débit d'une progéniture de pomme soumise à des contraintes hydriques

Author: Nicolas Virlet, Jean-Luc Regnard, Sébastien Martinez, Evelyne Costes, Audrey Jolivot, Pierre-Eric Lauri, David Gómez-Candón, Sylvain Labbé, Valentine Lebourgeois, Science du sol et environnement (MONTP UMR SOL ENVI), Institut National de la Recherche Agronomique (INRA), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Territoires, Environnement, Télédétection et Information Spatiale (UMR TETIS), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-AgroParisTech-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Montpellier SupAgro, Languedoc-Roussillon Region, Telerieg (Interreg IV) : SOE1/P2/E082, HiriFAP (Agropolis Fondation) : ANR-10-LABX-0001-01, Agreenium EcoHort programme, Consejo Superior de Investigaciones Científicas [Spain] (CSIC), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-AgroParisTech-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)
Subjects: 0106 biological sciences, IMAGERIE, variabilité génétique, 01 natural sciences, high spatial resolution, remote sensing, Evapotranspiration, Image resolution, malus domestica, Vegetal Biology, température de la feuille, [SDE.IE]Environmental Sciences/Environmental Engineering, déficit hydrique, télédétection aéroportée, drought stress, 04 agricultural and veterinary sciences, Vegetation, agriculture de précision, Agricultural sciences, photographie aérienne, Geography, Field trial, imagery, F60 - Physiologie et biochimie végétale, Apple tree, Horticulture, analyse d'image, Normalized Difference Vegetation Index, stress du à la sécheresse, Altitude, [SDV.SA.STA]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture, DEFICIT EN EAU, irrigation à la parcelle, TELEDETECTION, Remote sensing, STRESS HYDRIQUE, TEMPERATURE DE SURFACE, canopy surface temperature, genetic variability, image resolution, Malus × domestica, transpiration, water deficit index, Pixel, INFRAROUGE THERMIQUE, 15. Life on land, HAUTE RESOLUTION SPATIALE, transpiration des feuilles, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, imagerie haute resolution, H50 - Troubles divers des plantes, U30 - Méthodes de recherche, Biologie végétale, Sciences agricoles, 010606 plant biology & botany
Abstract: International audience; The genetic variability of fruit trees in response to drought stress is scarcely studied. As adaptation of scion cultivars to abiotic constraints constitutes a new challenge for fruit production, in particular where water scarcity is likely to occur, development of high-throughput phenotyping strategies applicable in the field to assess the tree response to soil drought among large populations is needed, overcoming the limitations of usual in-planta measurements. In this research, remotely sensed images were acquired by ultra-light aircraft (ULA) and an unmanned aerial vehicle (UAV) during 4 years in a field trial where an apple progeny (122 hybrids) was studied under contrasted summer irrigation regimes. Ortho-images were simultaneously acquired in visible (RGB), near-infrared (NIR) and thermalinfrared (TIR) bands. After rthorectification, georeferencing and mosaicking, RGB and NIR images were used to compute different vegetation indices over the field trial, while TIR imaging allowed extraction of the vegetation surface temperature (Ts), which was calibrated at ground level by using hot and cold reference targets. The Morans'f water deficit index (WDI), which combines the surface minus air temperature (Ts-Ta) and the normalized difference vegetation index (NDVI), was used as a stress phenotypic variable. WDI estimates the ratio of actual to maximal evapotranspiration (WDI=1.ETact/ETmax) in discontinuous plant covers. Like the Ts-Ta variable, it significantly discriminated the tree water statuses and genotypes. On the basis of different plant- and image-based indices, individual tree behaviour trends (isohydric vs. anisohydric) can be distinguished among the progeny, irrespective of tree vigour. This opens potential applications for plant breeding, and genetic bases of apple tree response to water stress are currently investigated through quantitative trait locus (QTL) detection. Making use of ULA with flights performed at 40-60 m altitude made it possible to strongly improve the TIR image resolution ('10 cm) and to limit the number of vegetation/soil mixed pixels. However, it will require careful image posttreatment, possibly including classification and/or segmentation.
Published: 2016
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13. ITK Vigne, a decision-support tool to adapt wine production to climate change, with or without irrigation

Author: Stoop, Philippe, Bsaibes, Aline, Gelly, Marc, Ojeda, Hernan, Lebon, Eric, Jourdan, Christophe, Trambouze, William, Laget, Frédéric, Ruetsch, Gabriel, Debiolles, Loïc, ITK, Unité expérimentale de Pech-Rouge (PECH ROUGE), Institut National de la Recherche Agronomique (INRA), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA), Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Chambre d'Agriculture de l'Hérault (CA 34), Association Climatique de l'Hérault (ACH), Vignobles Foncalieu, Netafim, ITKVigne is an output of Disp’eau, a collaborative project funded by the French 'Fonds Unique Interministériel', the Languedoc-Roussillon region, and FEDER., Unité d'oenologie de Pech-Rouge (MONTP PECH ROUG UE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Institut National de la Recherche Agronomique (INRA)-Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and ProdInra, Archive Ouverte
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDV.SA] Life Sciences [q-bio]/Agricultural sciences
Abstract: Climate change is an important challenge for most wine producing countries, since wine quality is closely linked to terroir, an interaction between soil, climate and training method. Hence a change in climate should induce a change in viticultural techniques, if one wants to keep the typicity of his appellation wines. In the Mediterranean region, the evolution towards warmer and drier summers has a strong and generally unfavourable influence on vineyard productivity (i.e.: yield and quality). Optimal vine water status dynamics have been therefore defined to produce distinctive wine profiles. However, the latter approach is rarely implemented in practice, since classical methods to measure grapevine water status are either too tedious or too expensive to be implemented on a large scale.To overcome those limits, a model-based decision support system (DSS), named iTKVigne, has been developed by a consortium led by ITK Company. The models included in this DSS were specifically adapted from former work done by INRA and CIRAD, and have been tested in the field from 2009 to 2013. After an initial calibration, this DSS has proven to be able to provide a very satisfactory estimation of pre-dawn water potential according to the soil, climate and vineyard training method at significantly lower cost than classical techniques. Unlike physical water status measurements, iTKVigne does not only allow a more accurate and water saving irrigation (irrigation quantities are generally reduced by about 15% when compared to current irrigation practices), it also allows adapting the training method according to the desired production objective, for winegrowers who cannot or do not want to irrigate. It may also be used as a tool to forecast on a production objective basis the future water requirements of new irrigation perimeters.
Published: 2015

14. Impact of the agglomeration process on structure and functional properties of the agglomerates based on the durum wheat semolina

Author: Sylvie Chevallier, Thierry Ruiz, Romain Jeantet, Bernard Cuq, Pierre Schuck, Sandra Mandato, Ines Hafsa, Serge Mejean, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Languedoc-Roussillon Region, which sponsored this study through the Chercheur d’Avenir 2010 project ‘‘Energy, Powder, and Interface’’. The authors would like to thank the Agence Nationale de la Recherche (ANR) for its financial support through the program ‘‘Reactive Powder’’., Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), and Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL)
Subjects: Materials science, Process (engineering), 02 engineering and technology, Starch gelatinization, 0404 agricultural biotechnology, 020401 chemical engineering, [SDV.IDA]Life Sciences [q-bio]/Food engineering, aliment en poudre, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, structure, 0204 chemical engineering, semoule, procédé industriel, business.industry, Economies of agglomeration, 04 agricultural and veterinary sciences, Microstructure, 040401 food science, Biotechnology, Shear rate, agglomération, Chemical engineering, Agglomerate, blé dur, Scientific method, propriété physicochimique, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract: UMR IATE Axe 2 : Structuration sous contraintes des agropolymères et réactivité des poudres; The agglomeration process controls the structure characteristics and end-use properties of the agglomerates.Understanding the mechanisms involved in the structuration of the food agglomerates still representsa real scientific stake. The objective of the present study is to improve the knowledge of theprocess–structure–properties relationships for the agglomeration of the durum wheat semolina. Pneumaticand mechanical agglomeration technologies were selected to generate different hydro–thermo–mechanical stresses during process. The agglomerates were characterized by their structures (electronmicroscopy, compactness, X-ray micro-tomography, starch gelatinization) and functional properties (colors,flowability, swelling, water solubility index). The results demonstrate specific effects of shear rate,process duration, and temperature on the physicochemical mechanisms occurring during processing. Afunctional mechanistic model was proposed to improve the description of the agglomeration mechanismsby considering the reversible and irreversible changes. This offers innovative potential to designnew agglomeration processes by considering the physicochemical reactivity of the food powders.
Published: 2015
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15. Towards a land management approach to ecological restoration toencourage stakeholder participation

Author: Héloïse Gonzalo-Turpin, Nathalie Couix, AGroécologie, Innovations, teRritoires (AGIR), Institut National de la Recherche Agronomique (INRA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Ecovars2 project, INRA-SAD, European Union, French government, Midi-Pyrenees region, Aquitaine region, and Languedoc-Roussillon region
Subjects: Process (engineering), media_common.quotation_subject, Geography, Planning and Development, ecological restoration, Land management, Management, Monitoring, Policy and Law, stakeholders, Perception, 11. Sustainability, Restoration ecology, Nature and Landscape Conservation, media_common, Land use, business.industry, Site manager, Environmental resource management, Pyrenees, Stakeholder, land management, Forestry, 15. Life on land, [SDE.ES]Environmental Sciences/Environmental and Society, interpretive approach, France, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, business
Abstract: International audience; In the field of ecological restoration, many authors call for more stakeholder participation in the processof restoration. In their opinion, when a restoration project is planned, the range of points of view andthe knowledge of local stakeholders need to be taken into account to limit the risk of failure. Althougheffective stakeholder involvement is often cited as a factor of success, in practice, it is far from systematic.To understand the ways in which the stakeholders actually participate in projects and their opinion ofthe projects, we analysed three restoration projects. We interviewed the people who would be affectedby the projects in the French Pyrenean Mountains: inhabitants, livestock farmers, and other users of theterritory, site managers, locally elected officials, experts, and development agents. Our results revealedthat how interviewees viewed the outcome and the success of a restoration project depended on their ownactivity, which also influenced the way they viewed and defined the territory concerned by restoration.Two different perceptions of ecological restoration objectives and approaches coexist in the Pyrenees. Thefirst is highly technical and the aim is simply to restore the original plant cover. In this case, the ‘territory’is limited to the area to be restored and its immediate surroundings. The second perception of restorationtakes into account both past and other possible land uses and consequently concerns a larger territoryand the users of the site to be restored. If the participation of local actors in the restoration process isdesired, we recommend a comprehensive land management approach to ecological restoration, as thisapproach is more likely to arouse the interest of the stakeholders.
Published: 2015
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16. Aromatic evolution of wine packed in virgin and recycled PET bottles

Author: Peggy Rigou, Clara Dombre, Pascale Chalier, Jérémie Wirth, Institut National de la Recherche Agronomique (INRA), Université de Montpellier (UM), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Sciences Pour l'Oenologie (SPO), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Bpi France (Herault, France), FEDER (Fonds Europeen de Developpement Regional), Languedoc-Roussillon Region (France), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Subjects: Rose, Wine ageing, white wine, Wine, Furfural, Analytical Chemistry, storage, chemistry.chemical_compound, Oxygen permeability, sensory characteristic, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Organic chemistry, Recycling, Ethyl pyruvate, Food science, volatile compound, Aroma, biology, Polyethylene Terephthalates, Chemistry, food and beverages, temperature, Sorption, General Medicine, red wine, biology.organism_classification, Chemical evolution, variety, PET, 13. Climate action, Aroma compounds, Odorants, impact, Glass, oxygen, Food Science
Abstract: The evolution of the aromatic profile of a rose wine packed in glass, virgin and recycled PET bottles was studied. Wine stored in PET and glass bottles was clearly differentiated after 5 months of storage but only by a limited number of compounds. More pronounced decrease of oxygen sensitive compounds such as methionol was observed in PET bottles as well as the apparition of oxidative and ageing aroma compounds such as ethyl pyruvate, furfural or dioxanes in higher concentration. Compared to virgin PET bottles, recycled PET bottles induced slight changes favouring the presence of esters and alcohols. The chemical evolution of wine was the most important phenomenon that explains the loss of flavour rather than the sorption into PET. Because of their moderate oxygen permeability, the use of virgin PET and recycled PET bottles could be adapted for short conservation of wine but detrimental to aromatic quality if long conservation is intended.
Published: 2015
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17. Serotonin: a new hope in Alzheimer's disease?

Author: Joël Bockaert, Sylvie Claeysen, Patrizia Giannoni, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Supported by grants from CNRS, INSERM, the French Research National Agency ANR (ANR-12-BSV4-008-01 ADAMGUARD project to J.B.), France Alzheimer Association (to S.C.), Soroptimist International (French Union, to S.C.), the Languedoc Roussillon Region ('Chercheuse d’Avenir'2012, to S.C.), and the Ligue Contre La Maladie d’Alzheimer (L.E.C.M.A., to SC)., and ANR-12-BSV4-0008,ADAMGUARD,Les réseaux protéiques associés aux récepteurs 5 HT4 : gardes rapprochées du trafic de l'ADAM10 et de l'APP(2012)
Subjects: Serotonin, Synaptic cleft, Physiology, Cognitive Neuroscience, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Disease, Biochemistry, Neuroprotection, 5-HT4 receptors, Amyloid beta-Protein Precursor, Serotonin 5-HT4 Receptor Agonists, Alzheimer Disease, medicine, Dementia, Animals, Humans, Nootropic Agents, Clinical Trials as Topic, Cell Biology, General Medicine, medicine.disease, Symptomatic relief, 3. Good health, Receptors, Serotonin, neuroprotection, Receptors, Serotonin, 5-HT4, Serotonin Antagonists, Psychology, 5-HT6 receptors, Neuroscience, Selective Serotonin Reuptake Inhibitors
Abstract: International audience; Alzheimer's disease (AD) is the most common form of dementia affecting 35 million individuals worldwide. Current AD treatments provide only brief symptomatic relief. It is therefore urgent to replace this symptomatic approach with a curative one. Increasing serotonin signaling as well as developing molecules that enhance serotonin concentration in the synaptic cleft have been debated as possible therapeutic strategies to slow the progression of AD. In this Viewpoint, we discuss exciting new insights regarding the modulation of serotonin signaling for AD prevention and therapy.
Published: 2015
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18. Contribution of high-resolution remotely sensed thermal-infrared imagery to high-throughput field phenotyping of an apple progeny submitted to water constraints

Author: Virlet, N., Gomez Candon, D., Lebourgeois, V.., Martinez, S., Jolivot, A., Lauri, P.E., Costes, E., Labbe, S., Regnard, J, Science du sol et environnement (MONTP UMR SOL ENVI), Institut National de la Recherche Agronomique (INRA), Consejo Superior de Investigaciones Científicas [Spain] (CSIC), Territoires, Environnement, Télédétection et Information Spatiale (UMR TETIS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-AgroParisTech-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-AgroParisTech-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Montpellier SupAgro, Languedoc-Roussillon Region, Telerieg (Interreg IV) : SOE1/P2/E082, HiriFAP (Agropolis Fondation) : ANR-10-LABX-0001-01, and Agreenium EcoHort programme
Subjects: TEMPERATURE DE SURFACE, remote sensing, high spatial resolution, INFRAROUGE THERMIQUE, [SDE.IE]Environmental Sciences/Environmental Engineering, [SDV.SA.STA]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture, drought stress, IMAGERIE, DEFICIT EN EAU, TELEDETECTION, imagery, HAUTE RESOLUTION SPATIALE, STRESS HYDRIQUE
Abstract: International audience; The genetic variability of fruit trees in response to drought stress is scarcely studied. As adaptation of scion cultivars to abiotic constraints constitutes a new challenge for fruit production, in particular where water scarcity is likely to occur, development of high-throughput phenotyping strategies applicable in the field to assess the tree response to soil drought among large populations is needed, overcoming the limitations of usual in-planta measurements. In this research, remotely sensed images were acquired by ultra-light aircraft (ULA) and an unmanned aerial vehicle (UAV) during 4 years in a field trial where an apple progeny (122 hybrids) was studied under contrasted summer irrigation regimes. Ortho-images were simultaneously acquired in visible (RGB), near-infrared (NIR) and thermalinfrared (TIR) bands. After rthorectification, georeferencing and mosaicking, RGB and NIR images were used to compute different vegetation indices over the field trial, while TIR imaging allowed extraction of the vegetation surface temperature (Ts), which was calibrated at ground level by using hot and cold reference targets. The Morans'f water deficit index (WDI), which combines the surface minus air temperature (Ts-Ta) and the normalized difference vegetation index (NDVI), was used as a stress phenotypic variable. WDI estimates the ratio of actual to maximal evapotranspiration (WDI=1.ETact/ETmax) in discontinuous plant covers. Like the Ts-Ta variable, it significantly discriminated the tree water statuses and genotypes. On the basis of different plant- and image-based indices, individual tree behaviour trends (isohydric vs. anisohydric) can be distinguished among the progeny, irrespective of tree vigour. This opens potential applications for plant breeding, and genetic bases of apple tree response to water stress are currently investigated through quantitative trait locus (QTL) detection. Making use of ULA with flights performed at 40-60 m altitude made it possible to strongly improve the TIR image resolution ('10 cm) and to limit the number of vegetation/soil mixed pixels. However, it will require careful image posttreatment, possibly including classification and/or segmentation.
Published: 2014
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19. Courtship behavior of the castniid palm borer, Paysandisia archon: Potential roles of male scents and visual cues in aday-flying moth

Author: Brigitte Frérot, Roxane Delle-Vedove, Martine Hossaert-McKey, Laurence Beaudoin-Ollivier, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Physiologie de l'Insecte : Signalisation et Communication (PISC), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National Agronomique Paris-Grignon (INA P-G), Bioagresseurs, analyse et maîtrise du risque (UPR Bioagresseurs), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), 'Languedoc-Roussillon Region,' CIRAD, 'GDR on Chemical Ecology' (CNRS/INEE) [G2827], Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Subjects: Male, 0106 biological sciences, [SDV]Life Sciences [q-bio], Moths, 01 natural sciences, Courtship, Sexual Behavior, Animal, Sex Attractants, mate choice, Mating, Elaeis guineensis, media_common, Introduction d'animaux, Ravageur des plantes, biology, Ecology, Phéromone sexuelle, General Medicine, mating, Mate choice, Papers, [SDE]Environmental Sciences, Pheromone, Female, L20 - Écologie animale, media_common.quotation_subject, Paysandisia archon, behavioral sequences, Zoology, 010603 evolutionary biology, Animals, male pheromones, Sensory cue, Courtship display, Comportement sexuel, Castniidae, Taxonomie, biology.organism_classification, H10 - Ravageurs des plantes, 010602 entomology, Insect Science
Abstract: International audience; The castniid palm borer, Paysandisia archon (Burmeister) (Lepidoptera: Castniidae), is a South American moth that in the last ten years has become a major pest of palm trees in the Mediterranean region. Current knowledge on the reproductive behavior of this diurnal moth suggests the importance of both visual and chemical cues, in particular the production of a male pheromone emitted during a specific scratching behavior. Male-produced scents have diverse functions in lepidopteran sexual communication but generally act during courtship behavior, leading to complex, stereotyped courtship sequences. As a first step to understand the cues involved in mating behavior and the role of male scents in male mating success, we quantified sequences of P. archon courtship behavior using video filming. To distinguish behaviors leading to an approach of both partners from those involved in short-range courtship, sequences were divided into "approach" and "interaction" phases. Quantifications and analyses were first made by NPMANOVA analysis of behavioral event frequencies, followed by flowchart construction using transition matrix probabilities. In 90% of the observations, courting activities led to copulation, but successful sequences were highly variable and could be divided into two categories, "rapid" and "prolonged" courtship sequences. In both categories, approaches were performed by males but depended strongly on female movements, especially on female flights. The significant behavioral differences were observed after the first contact (i.e., interaction phase) where, in rapid sequences, males generally acceded to copulation without displaying scratching behavior. Conversely, in prolonged sequences, the female expressed evading behavior and male scratching frequency increased. The possible roles of male scent emission in female mate choice and the importance of visual cues in the mating behavior of P. archon are discussed.
Published: 2014
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20. SAP97-mediated ADAM10 trafficking from Golgi outposts depends on PKC phosphorylation

Author: Saraceno, C, Marcello, E, Di Marino, D, Borroni, B, Claeysen, S, Perroy, J, Padovani, A, Tramontano, A, Gardoni, F, Di Luca, M, Fondazione Cenci Bolognetti, Istituto Pasteur, BLANC - Les réseaux protéiques associés aux récepteurs 5 HT4 : gardes rapprochées du trafic de l'ADAM10 et de l'APP - - ADAMGUARD2012 - ANR-12-BSV4-0008 - BLANC - VALID, Cell permeable peptides as drug delivery system: a way towards innovative therapeutic strategies for neurodegeneration - CPADS - - EC:FP7:PEOPLE2008-03-01 - 2012-02-29 - 217902 - VALID, Department of Pharmacological Sciences and Biomolecular, Università degli Studi di Milano = University of Milan (UNIMI), Department of Physics [Roma La Sapienza], Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), University of Brescia, Institut de Génomique Fonctionnelle (IGF), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP), Acknowledgement is made to the donors of the ADR, a program of the BrightFocus Foundation, for support of this research. This work was supported by grants from the European Union's Seventh Framework Program (FP7 2007-2013, Grant Agreement no. PIAP-GA-2008-217902) (to MdL), from Fondazione CARIPLO (project no. 2319-2008) (to MdL), from FIRB-Accordi di Programma (project code RBAP11HSZS) (to MdL), from PRIN 2010PWNJXK (to MdL), from Veronesi Foundation Young Investigator Research Programme 2013 (to EM), from the Alzheimer's Association (NIRP-14-304969) (to EM) and an ADR program research fellowship (to EM). SC acknowledges support from the France Alzheimer Association, the Languedoc Roussillon Region and the French Research National Agency ANR (ANR-12-BSV4-008-01 ADAMGUARD project)., ANR-12-BSV4-0008,ADAMGUARD,Les réseaux protéiques associés aux récepteurs 5 HT4 : gardes rapprochées du trafic de l'ADAM10 et de l'APP(2012), European Project: 217902,EC:FP7:PEOPLE,FP7-PEOPLE-2007-3-1-IAPP,CPADS(2008), University of Milan, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), and Dipartimento di Fisica [Roma La Sapienza]
Subjects: Models, Molecular, MESH: Phosphothreonine, Golgi Apparatus, MESH: Amino Acid Sequence, MESH: Synapses, Discs Large Homolog 1 Protein, ADAM10 Protein, Chlorocebus aethiops, MESH: Animals, Phosphorylation, Protein Kinase C, MESH: COS Cells, Phosphothreonine, MESH: HEK293 Cells, COS Cells, MESH: Post-Synaptic Density, Original Article, MESH: Membrane Proteins, MESH: Models, Molecular, Protein Binding, MESH: ADAM Proteins, MESH: Enzyme Activation, MESH: Rats, Molecular Sequence Data, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, MESH: Golgi Apparatus, Alzheimer Disease, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Animals, Humans, MESH: Protein Binding, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Amino Acid Sequence, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Adaptor Proteins, Signal Transducing, MESH: Adaptor Proteins, Signal Transducing, MESH: Humans, MESH: Molecular Sequence Data, MESH: Phosphorylation, Membrane Proteins, Post-Synaptic Density, MESH: Cercopithecus aethiops, MESH: Protein Kinase C, Rats, Enzyme Activation, ADAM Proteins, HEK293 Cells, MESH: Amyloid Precursor Protein Secretases, Synapses, Amyloid Precursor Protein Secretases, Cercopithecus aethiops, MESH: Alzheimer Disease
Abstract: International audience; A disintegrin and metalloproteinase 10 (ADAM10) is the major α-secretase that catalyzes the amyloid precursor protein (APP) ectodomain shedding in the brain and prevents amyloid formation. Its activity depends on correct intracellular trafficking and on synaptic membrane insertion. Here, we describe that in hippocampal neurons the synapse-associated protein-97 (SAP97), an excitatory synapse scaffolding element, governs ADAM10 trafficking from dendritic Golgi outposts to synaptic membranes. This process is mediated by a previously uncharacterized protein kinase C phosphosite in SAP97 SRC homology 3 domain that modulates SAP97 association with ADAM10. Such mechanism is essential for ADAM10 trafficking from the Golgi outposts to the synapse, but does not affect ADAM10 transport from the endoplasmic reticulum. Notably, this process is altered in Alzheimer's disease brains. These results help in understanding the mechanism responsible for the modulation of ADAM10 intracellular path, and can constitute an innovative therapeutic strategy to finely tune ADAM10 shedding activity towards APP.
Published: 2014
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21. Projecting future drought in mediterranean forests: bias correction of climate models matters!

Author: Julien Ruffault, Fabien Goge, Florent Mouillot, Nicolas Martin-StPaul, Carole Duffet, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Ecologie Systématique et Evolution (ESE), Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), and This work is a contribution to the UE 7th FP Env.1.3.1.1 FUME 'Forest fires under climate, social and economic changes in Europe, the Mediterranean and other fire-affected areas of the world', grant agreement no. 243888. A doctoral research grant was provided to JR by the Languedoc-Roussillon region, the Centre National de la Recherche Scientifique, and the ANR project SCION (no. ANR-09-PEXT-006) and a postdoctoral grant to CD by the ANR project MESOEROS (grant agreement no. ANR-06-VULN-012). A postdoctoral grant to NKM-SP was provided by the HUMBOLDT project, which is part of the GIS Climate-Environment-Society consortium.
Subjects: Atmospheric Science, FORET, [SDE.MCG]Environmental Sciences/Global Changes, BILAN HYDRIQUE, Climate change, Forcing (mathematics), METHODE, Water balance, ETUDE COMPARATIVE, Precipitation, DISTRIBUTION SPATIALE, TEMPERATURE, SECHERESSE, Anomaly (natural sciences), PREVISION CLIMATIQUE, ETUDE REGIONALE, Vegetation, 15. Life on land, PREVISION, DEFICIT HYDRIQUE, 13. Climate action, Climatology, PLUIE, PRECIPITATION, CHANGEMENT CLIMATIQUE, MODELE CLIMATIQUE, Common spatial pattern, Environmental science, RADIATION, Climate model, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract: International audience; Global and regional climate models (GCM and RCM) are generally biased and cannot be used as forcing variables in ecological impact models without some form of prior bias correction. In this study, we investigated the influence of the bias correction method on drought projections in Mediterranean forests in southern France for the end of the twenty-first century (2071-2100). We used a water balance model with two different atmospheric climate forcings built from the same RCM simulations but using two different correction methods (quantile mapping or anomaly method). Drought, defined here as periods when vegetation functioning is affected by water deficit, was described in terms of intensity, duration and timing. Our results showed that the choice of the bias correction method had little effects on temperature and global radiation projections. However, although both methods led to similar predictions of precipitation amount, they induced strong differences in their temporal distribution, especially during summer. These differences were amplified when the climatic data were used to force the water balance model. On average, the choice of bias correction leads to 45 % uncertainty in the predicted anomalies in drought intensity along with discrepancies in the spatial pattern of the predicted changes and changes in the year-to-year variability in drought characteristics. We conclude that the choice of a bias correction method might have a significant impact on the projections of forest response to climate change
Published: 2014
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22. Comparative Evolution of Oxygen, Carbon Dioxide, Nitrogen, and Sulfites during Storage of a Rose Wine Bottled in PET and Glass

Author: Jean-Michel Salmon, Marie Toussaint, Jean-Claude Vidal, Unité expérimentale de Pech-Rouge (PECH ROUGE), Institut National de la Recherche Agronomique (INRA), OSEO (French state agency for innovation, Herault, France), ERDF (European Regional Development Funds, European Community), and Languedoc-Roussillon Region
Subjects: MECHANISM, business.product_category, packaging gas balance, chemistry.chemical_element, Shelf life, OXIDATION, 01 natural sciences, nitrogen, Polyethylene Glycols, Bottling line, chemistry.chemical_compound, 0404 agricultural biotechnology, POLYPHENOLS, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Polyethylene terephthalate, Bottle, Sulfites, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, wine, glass, Wine, Polyethylene Terephthalates, 010401 analytical chemistry, Food Packaging, Temperature, carbon dioxide, 04 agricultural and veterinary sciences, General Chemistry, Partial pressure, bottle, Pulp and paper industry, 040401 food science, Nitrogen, 0104 chemical sciences, SULFUR-DIOXIDE, PET, chemistry, Food Storage, 13. Climate action, Carbon dioxide, shelf life, General Agricultural and Biological Sciences, business, oxygen
Abstract: International audience; The management of dissolved and headspace gases during bottling and the choice of packaging are both key factors for the shelf life of wine. Two kinds of 75 cL polyethylene terephthalate (PET) bottles (with or without recycled PET) were compared to glass bottles filled with a rose wine, closed with the same screwcaps and stored upright at 20 degrees C in light or in the dark. Analytical monitoring (aphrometric pressure, headspace volume, O-2, N-2, CO2, and SO2) was carried out for 372 days. After the consumption of O-2 trapped during bottling, the total O-2 content in glass bottles remained stable. A substantial decrease of CO2 and SO2 concentration and an increase of O-2 concentration were observed in the PET bottles after 6 months because of the considerable gas permeability of monolayer PET. Light accelerated O-2 consumption during the early months. Finally, the kinetic monitoring of partial pressures in gas and liquid phases in bottles showed contrasting behavior of O-2 and N-2 in comparison with CO2.
Published: 2014
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23. Description of internal microstructure of agglomerated cereal powders using X-ray microtomography to study of process-structure relationships

Author: Thierry Ruiz, Sylvie Chevallier, Su Jin Kim, Bernard Cuq, Alain Le Bail, Ines Hafsa, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL), and Languedoc-Roussillon Region
Subjects: X-ray microtomography, Morphology (linguistics), Materials science, General Chemical Engineering, Mixing (process engineering), WET GRANULATION, Food agglomerates, TOMOGRAPHY, [SDV.IDA]Life Sciences [q-bio]/Food engineering, PARTICLES, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Composite material, Porosity, BINDER PROPERTIES, Internal microstructure, 2. Zero hunger, Shearing (physics), FOOD POWDERS, Couscous, Economies of agglomeration, POROSITY, Microstructure, OPPORTUNITIES, Crystallography, FLUIDIZED-BED, Agglomerate, MORPHOLOGY, X-ray micro-computed tomography, NUCLEATION
Abstract: International audience; Agglomerated food powders are made from native particles which are assembled to form grains. The present investigation seeks to contribute to a better understanding of the three-dimensional internal morphology of food agglomerates (i.e. different couscous grains made from durum wheat semolina under different agglomeration process conditions) by means of microscopic methods (size, compactness) and X-ray micro-computed tomography (XMT), to bring understanding of the process-structure relationships. The three-dimensional internal morphology of food agglomerates was evaluated by means of X-ray micro-computed tomography (XMT), using a tabletop system with a resolution of several micrometers. XMT was considered to evaluate the internal porosity (or void fraction) as well as the size and spatial distribution of pores inside the grains. Closed porosity values ranged between 0.005 and 0.011. Diameter of internal pores was found to range between 13 and 155 mu m which contribute to control mainly their end-properties. The experimental results were discussed to develop a better knowledge of the process-structure relationships for food agglomerates based on wheat powders, produced by wet agglomeration process. We demonstrated that the shearing conditions during the initial mixing stage significantly contribute to the internal structure characteristics of agglomerates. The cooking treatment is found to largely improve the mechanical strength of the agglomerates.
Published: 2014
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24. Side scatter intensity is highly heterogeneous in undifferentiated pluripotent stem cells and predicts clonogenic self-renewal.: Side scatter establishes a new PSC hierarchy

Author: Ramirez, Jean-Marie, Bai, Qiang, Péquignot, Marie, Becker, Fabienne, Kassambara, Alboukadel, Bouin, Alexandra, Kalatzis, Vasiliki, Dijon-Grinand, Marilyne, de Vos, John, Cellules souches normales et cancéreuses, Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut de recherche en biothérapie (IRB), Université Montpellier 1 (UM1)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut des Neurosciences de Montpellier (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Association Française contre les Myopathies (AFM, Paris), The Languedoc-Roussillon Region (Chercheur d'Avenir 09-13198-01), and Frei, Monique
Subjects: side scatter, [SDV.IMM] Life Sciences [q-bio]/Immunology, clonogenic self-renewal, [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.IMM]Life Sciences [q-bio]/Immunology, pluripotent stem cells, heterogeneity, skin and connective tissue diseases, pluripotency, stem cell hierarchies, [SDV.BDD]Life Sciences [q-bio]/Development Biology
Abstract: International audience; In culture, human pluripotent stem cells (PSCs) are phenotypically (for instance, the SSEA3 expression level) and functionally (capacity to survive after single-cell dissociation) heterogeneous. We report here that the side scatter (SSC) signal measured by flow cytometry, a variable correlated with membrane irregularity and cell granularity, is very high in PSCs, even higher than in blood polymorphonuclear cells, and markedly heterogeneous. Moreover, SSC intensity rapidly and strongly decreases upon PSC differentiation into any of the three germ layers. PSCs with high SSC (HSSC cells) or low SSC (LSSC cells) values both express pluripotency markers, but HSSC cells are characterized by more frequent simultaneous expression of the membrane pluripotency factors SSEA3, SSEA4, TRA-1-81, TRA-1-60, and CD24 and by a higher mitochondrial content. Functionally, HSSC cells are more likely to generate colonies upon single-cell passage than LSSC cells. SSC monitoring might provide a simple, but robust and rapid method to estimate pluripotency variations in culture and unveils a new phenotypic and functional heterogeneity in PSCs.
Published: 2013
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25. Plasticity of Hypothalamic Dopamine Neurons during Lactation Results in Dissociation of Electrical Activity and Release

Author: Stephen J. Bunn, Dave R. Grattan, Anne Guillou, Paul Le Tissier, Patrice Mollard, Sébastien Parnaudeau, Nicola Romanò, David J. Hodson, Siew Hoong Yip, Xavier Bonnefont, François Tronche, Agnès O. Martin, Siobhan E Kirk, Expression des Gènes et comportement adaptatifs = Molecular Genetics, Neurophysiology and Behavior (NPS-15), Neuroscience Paris Seine (NPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), National Agency of Research [DAT-NET 2007], National Institute of Health and Medical Research, National Center of Scientific Research, Montpellier University 1, Montpellier University 2, Foundation for Medical Research, National Network of Genopoles, Federative Research Institutes, Languedoc-Roussillon Region, Neurosciences Paris Seine (NPS), Physiopathologie des Maladies du Système Nerveux Central, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Male, Patch-Clamp Techniques, RNA, Untranslated, Dopamine, Dopamine Plasma Membrane Transport Proteins, [SDV]Life Sciences [q-bio], Action Potentials, Mice, 0302 clinical medicine, Lactation, Benz(a)Anthracenes, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, Neuronal Plasticity, General Neuroscience, Articles, medicine.anatomical_structure, Median eminence, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], hormones, hormone substitutes, and hormone antagonists, medicine.drug, medicine.medical_specialty, endocrine system, Biophysics, Radioimmunoassay, Mice, Transgenic, In Vitro Techniques, Biology, 03 medical and health sciences, Bacterial Proteins, Internal medicine, medicine, Animals, Patch clamp, 030304 developmental biology, Analysis of Variance, Tyrosine hydroxylase, Dopaminergic Neurons, Proteins, Electric Stimulation, Prolactin, Luminescent Proteins, Endocrinology, Hypothalamic Area, Lateral, Vesicular Monoamine Transport Proteins, Neuron, 030217 neurology & neurosurgery
Abstract: International audience; Tuberoinfundibular dopamine (TIDA) neurons are the central regulators of prolactin (PRL) secretion. Their extensive functional plasticity allows a change from low PRL secretion in the non-pregnant state to the condition of hyperprolactinemia that characterizes lactation. To allow this rise in PRL, TIDA neurons are thought to become unresponsive to PRL at lactation and functionally silenced. Here we show that, contrary to expectations, the electrical properties of the system were not modified during lactation and that the neurons remained electrically responsive to a PRL stimulus, with PRL inducing an acute increase in their firing rate during lactation that was identical to that seen in non-pregnant mice. Furthermore, we show a long-term organization of TIDA neuron electrical activity with an harmonization of their firing rates, which remains intact during lactation. However, PRL-induced secretion of dopamine (DA) at the median eminence was strongly blunted during lactation, at least in part attributable to lack of phosphorylation of tyrosine hydroxylase, the key enzyme involved in DA synthesis. We therefore conclude that lactation, rather than involving electrical silencing of TIDA neurons, represents a condition of decoupling between electrical activity at the cell body and DA secretion at the median eminence.
Published: 2013
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26. In-line monitoring of durum wheat semolina wet agglomeration by near infrared spectroscopy for different water supply conditions and water addition levels

Author: Thierry Ruiz, Bernard Cuq, Sandra Mandato, Abderrahmane Ait-Kaddour, Caio Cesar Taliani, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Unite Rech CALITYSS Consommateur, UPSP, Languedoc-Roussillon Region, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)
Subjects: Water flow, [SDV]Life Sciences [q-bio], Analytical chemistry, Principal component analysis, PROTEIN, 02 engineering and technology, GRANULATION PROCESSES, MOISTURE, 01 natural sciences, GRAIN, CHEMOMETRICS, Chemometrics, Multivariate data analysis, Spectroscopy, Water content, Moisture, SECONDARY STRUCTURE, Chemistry, Economies of agglomeration, 010401 analytical chemistry, 021001 nanoscience & nanotechnology, Wet agglomeration, 6. Clean water, PARTICLE-SIZE, 0104 chemical sciences, Agglomerate, Wheat-based food powder, Particle size, 0210 nano-technology, Near infrared spectroscopy, Food Science, NUCLEATION
Abstract: The aim of this work is (i) to study the ability of near infrared (NIR) spectroscopy to follow in-line the agglomeration process of durum wheat semolina using mechanical mixer according to two different water supply conditions (i.e. high versus low water flow rates) and to four different water addition levels, and (ii) to describe the associated structural changes of agglomerates. The results showed that analyses of spectral variations can be useful to discriminate different agglomeration processes, which are associated to different kinetic changes in terms of agglomerate size distribution and water content per size fraction. Principal component analysis (PCA) has been used to qualitatively describe physical and chemical variations occurring during the wet agglomeration mechanisms and to identify specific kinetics and characteristic times. Whatever the water supply condition, kinetic variations of principal components (PCs) on raw and second derivative NIR spectra are observed. Characteristic times have been identified on PC scores and an attempt to link these times to changes of agglomerates physical and chemical properties have been carried out, mainly based on the analysis of associated loading spectra. (C) 2013 Elsevier Ltd. All rights reserved.
Published: 2013
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27. An experimental test of the transmission-virulence trade-off hypothesis in a plant virus

Author: Doumayrou, Juliette, Avellan, Astrid, Froissart, Rémy, Michalakis, Yannis, Biologie et Génétique des interactions Plantes-parasites pour la Protection Intégrée, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Montpellier 2 - Sciences et Techniques (UM2), Centre national de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA SPE), Languedoc-Roussillon Region, CNRS, and Institut de Recherche pour le Developpement (IRD)
Subjects: virulence, CaMV, plant virus, [SDV]Life Sciences [q-bio], transmission, within-host accumulation, trade-off
Abstract: BGPI : équipe 2; International audience; The transmissionvirulence trade-off hypothesis is one of the few adaptive explanations of virulence evolution, and assumes that there is an overall positive correlation between parasite transmission and virulence. The shape of the transmissionvirulence relationship predicts whether virulence should evolve toward either a maximum or to an intermediate optimum. A positive correlation between each of these traits and within-host growth is often suggested to underlie the relationship between virulence and transmission. There are few experimental tests of this hypothesis; this study reports on the first empirical test on a plant pathogen. We infected Brassica rapa plants with nine natural isolates of Cauliflower mosaic virus and then estimated three traits: transmission, virulence, and within-host viral accumulation. As predicted by the trade-off hypothesis, we observed a positive correlation between transmission and virulence, suggestive of the existence of an intermediate optimum. We discovered the unexpected existence of two groups of within-host accumulation, differing by at least an order of magnitude. When accumulation groups were not accounted for, within-host accumulation was correlated neither to virulence nor transmission, although our results suggest that within each group these correlations exist.
Published: 2013
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28. Molecular identification and ecological characteristics of two cryptic lineages within a cosmopolitan aphid pest, Brachycaudus helichrysi (Hemiptera: Aphididae)

Author: Flavie Vanlerberghe-Masutti, Emmanuelle Jousselin, A. Coeur d’acier, Jean-Pierre Rossi, Gwenaëlle Genson, Josephine Piffaretti, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), ANR [JCJC-05], SPE Department of INRA, INRA-SPE, and Languedoc Roussillon region
Subjects: 0106 biological sciences, Entomology, Lineage (evolution), Climate, [SDV]Life Sciences [q-bio], ved/biology.organism_classification_rank.species, Biology, 010603 evolutionary biology, 01 natural sciences, Asexuality, 03 medical and health sciences, Lineage, Botany, 030304 developmental biology, 0303 health sciences, Aphid, PCA, Obligate, ved/biology, Ecology, fungi, food and beverages, Aphididae, biology.organism_classification, Hemiptera, Host plant association, Insect Science, Aphids, PEST analysis, Brachycaudus helichrysi
Abstract: International audience; The leaf-curl plum aphid, Brachycaudus helichrysi (Kaltenbach 1843), is a polyphagous cosmopolitan aphid. This serious pest of plum and peach orchards affects the growth of its host plants by curling infested leaves, and can transmit viruses such as the plum pox virus. Phylogenetic analyses have recently shown that B. helichrysi actually comprises two lineages that are morphologically similar but genetically different. In this study, we developed a PCR-RFLP test for differentiating between these two lineages, based on a mitochondrial DNA fragment. We used this test to investigate the patterns of host plant association and the geographical distribution of these lineages, with a large sample of individuals collected from numerous host plants and locations worldwide. We found that one of the lineages was never present on plum trees, and that individuals from both lineages coexisted on many secondary herbaceous hosts at almost all of the sites studied. We then carried out principal component analysis to determine whether the distribution of the two lineages could be explained by climatic features. We found that the lineage absent from plum trees was not found in regions with harsh winters. This suggests that one of the lineages within B. helichrysi may be an obligate parthenogenetic aphid.
Published: 2013
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29. Description of Culicoides paradoxalis sp nov from France and Portugal (Diptera: Ceratopogonidae)

Author: Ramilo, David, Garros, Claire, Mathieu, Bruno, Benedet, Christophe, Allene, Xavier, Silva, Elisabete, Alexandre-Pires, Graca, Da Fonseca, Isabel Pereira, Simon Carpenter, Radrova, Jana, Delecolle, Jean-Claude, Universidade Técnica de Lisboa, Contrôle des maladies animales exotiques et émergentes (UMR CMAEE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Dynamique des interactions Hôte pathogène, Université de Strasbourg (UNISTRA), Pirbright Institute, Faculty of Science, Suez Canal University. Ismailia. Egypt, Ministere en charge de l'Agriculture (France), convention Chercheur d'Avenir from Languedoc-Roussillon region (France), Defra national laboratory for Culicoides in the United Kingdom, and Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)
Subjects: Identification, Insecta, Arthropoda, [SDV]Life Sciences [q-bio], L73 - Maladies des animaux, Cytochrome c oxydase, Ceratopogonidae, taxonomy, Animalia, Cytochrome Oxidase I, Diptera, fungi, Culicoides, Taxonomie, Biodiversity, biting midges, Espèce nouvelle, L72 - Organismes nuisibles des animaux
Abstract: International audience; A new species, Culicoides paradoxalis Ramilo and Delecolle (Diptera: Ceratopogonidae), is described from specimens collected in France (Corsica and southeast region) and Portugal. This species resembles Culicoides lupicaris Downes and Kettle, and can be distinguished from this species and from Culicoides newsteadi Austen by its wing pattern, in addition to the absence of spines on the tarsomere 4 of female mid leg. In male, the presence of two appendices on the sternite 9 together with the absence of sensilla coeloconica on the flagellomere 11 is also useful to distinguish these three species. Separation from other members of the Culicoides subgenus is confirmed by the analysis of the Cytochrome Oxidase I (COI) mitochondrial marker.
Published: 2013
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30. Rheological and mechanical characterization of wet agglomerates processed in low shear mixer

Author: Thierry Ruiz, Bernard Cuq, Eric Rondet, Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Languedoc-Roussillon Region through the Chercheur d'Avenir project 'Energy, Powder and Interface', Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)
Subjects: Materials science, Mechanical characterization, Plasticity, Rheometer, [SDV]Life Sciences [q-bio], Population, SOLIDS, 02 engineering and technology, PARAMETERS, 020401 chemical engineering, Rheology, BINDER, Cohesion (geology), Porous materials, Internal friction, 0204 chemical engineering, Composite material, education, Shearing (physics), education.field_of_study, Economies of agglomeration, Bulk deformation, 021001 nanoscience & nanotechnology, POWDERS, OPPORTUNITIES, Agglomerate, Strain measurement, COMPRESSION, Wetting, 0210 nano-technology, Food Science, KAWAKITA
Abstract: The mechanical and rheological characterization of structures processed by wet agglomeration is a way to study their structural organization. The aim of this study is to submit and discuss the results of mechanical and rheological characterization of wet agglomerates obtained by wetting cereal powder in low shear mixer. For various conditions of wetting, these properties are obtained thanks to shearing and compression tests led on a powder rheometer (FT4 - Freeman Technology, UK). The analysis of the mechanical and rheological properties of agglomerates obtained after wet agglomeration process has been carried out thanks to an approach based on soil science and granular media. This approach makes it possible to highlight the great influence of water content on the rheological behavior of the agglomerates bed bulk: Its increase results in variations of the friction between grains together with variations in their cohesion. These variations are analyzed regarding the modifications in the population balance induced by the increase in water content. Uniaxial compression tests make it possible to calculate, through three compression models (Kawakita, Hackel and Adams), the agglomerates strength. It is shown that it evolves according to a decreasing power law when water content increases. These results are in accordance with the fractal description of the agglomeration process that support a decrease of the agglomerates solid volume fraction, and thus of their resistance, with water content. (c) 2013 Elsevier Ltd. All rights reserved.
Published: 2013
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31. Pharmacological profile of engineered 5-HT4 receptors and identification of 5-HT4 receptor-biased ligands

Author: Joël Bockaert, Aline Dumuis, Maud Cochet, Emilie Queffeulou, Florence Gaven, Lucie P. Pellissier, Sylvie Claeysen, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), This work was financially supported by grants from CNRS, INSERM, the French Minister of Research (ANR Blanc 2006, to AD and SC), the France Alzheimer association (to SC), the Soroptimist International (French Union, to SC) and the Languedoc Roussillon region ('Chercheuse d'Avenir' 2012, to SC)., and ANR-06-BLAN-0087,GPCR dimers,Molecular and dynamic aspects of G-protein coupled receptor dimers functioning(2006)
Subjects: Agonist, inositol phosphate, G protein, medicine.drug_class, biased ligand, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Biology, Partial agonist, 03 medical and health sciences, 0302 clinical medicine, medicine, cyclic AMP, G protein-coupled receptor, 5-HT4 receptor, Receptor, Molecular Biology, 5-HT receptor, 030304 developmental biology, 0303 health sciences, General Neuroscience, Wild type, Biochemistry, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Neurology (clinical), Signal transduction, 030217 neurology & neurosurgery, Developmental Biology
Abstract: G protein-coupled receptors (GPCRs) can activate simultaneously multiple signaling pathways upon agonist binding. The combined use of engineered GPCRs, such as the receptors activated solely by synthetic ligands (RASSLs), and of biased ligands that activate only one pathway at a time might help deciphering the physiological role of each G protein signaling. In order to find serotonin type 4 receptor (5-HT₄R) biased ligands, we analyzed the ability of several compounds to activate the Gs and G(q/11) pathways in COS-7 cells that transiently express wild type 5-HT₄R, the 5-HT₄R-D(100)A mutant (known also as 5-HT₄-RASSL, or Rs1) or the 5-HT₄R-T(104)A mutant, which modifies agonist-induced 5-HT₄R activation. This analysis allowed completing the pharmacological profile of the two mutant 5-HT₄Rs, but we did not find any biased ligand for the mutant receptors. Conversely, we identified the first biased agonists for wild type 5-HT₄R. Indeed, RS 67333 and prucalopride acted as partial agonists to induce cAMP accumulation, but as antagonists on inositol phosphate production. Moreover, they showed very different antagonist potencies that could be exploited to study the activation of the G(s) pathway, with or without concomitant block of G(q/11) signaling. This article is part of a Special Issue entitled Optogenetics (7th BRES).
Published: 2013
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32. Early administration of RS 67333, a specific 5-HT4 receptor agonist, prevents amyloidogenesis and behavioral deficits in the 5XFAD mouse model of Alzheimer’s disease

Author: Patrizia eGiannoni, Florence eGaven, Dimitri eDe Bundel, Kevin eBaranger, Evelyne eMarchetti-Gauthier, François S Roman, Emmanuel eValjent, Philippe eMarin, Joël eBockaert, Santiago eRivera, Sylvie eClaeysen, Institut de Génomique Fonctionnelle (IGF), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU), Hôpital de la Timone [CHU - APHM] (TIMONE), Neurobiologie des interactions cellulaires et neurophysiopathologie - NICN (NICN), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Supported by grants from CNRS, INSERM, the French Research National Agency ANR (ANR-12-BSV4-008-01 ADAMGUARD project to JB), Fondation pour la Recherche Médicale en France (FRM to DDB), France Alzheimer Association (to SC), Soroptimist International (French Union, to SC), the Languedoc Roussillon Region ('Chercheuse d'Avenir' 2012, to SC) and the Ligue Contre La Maladie d'Alzheimer (L.E.C.M.A., to SC). SR acknowledges support from the ANR (ANR-08-MNPS-042-04 TIMPAD project) France Alzheimer Association and L.E.C.M.A. KB acknowledges support from the French Alzheimer’s Plan., ANR-12-BSV4-0008,ADAMGUARD,Les réseaux protéiques associés aux récepteurs 5 HT4 : gardes rapprochées du trafic de l'ADAM10 et de l'APP(2012), ANR-08-MNPS-0042,TIMPAD,Interactions fonctionnelles entre le système TIMP/MMP et le LRP-1 dans la maladie d'Alzheimer : identification de nouvelles cibles thérapeutiques(2008), Giannoni, Patrizia, BLANC - Les réseaux protéiques associés aux récepteurs 5 HT4 : gardes rapprochées du trafic de l'ADAM10 et de l'APP - - ADAMGUARD2012 - ANR-12-BSV4-0008 - BLANC - VALID, Maladies Neurologiques et Psychiatriques - Interactions fonctionnelles entre le système TIMP/MMP et le LRP-1 dans la maladie d'Alzheimer : identification de nouvelles cibles thérapeutiques - - TIMPAD2008 - ANR-08-MNPS-0042 - MNP - VALID, Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), and Experimental Pharmacology
Subjects: Agonist, medicine.medical_specialty, Aging, medicine.drug_class, amyloid plaques, Cognitive Neuroscience, 5-HT4 receptor, lcsh:RC321-571, Pathogenesis, 03 medical and health sciences, 0302 clinical medicine, 5xFAD mouse, Internal medicine, medicine, Amyloid precursor protein, 5HT4-receptors, Original Research Article, G protein-coupled receptor, Receptor, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, 030304 developmental biology, 0303 health sciences, biology, business.industry, [SCCO.NEUR]Cognitive science/Neuroscience, [SCCO.NEUR] Cognitive science/Neuroscience, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, medicine.disease, Receptor antagonist, Astrogliosis, serotonin, Endocrinology, preventive pharmacotherapy, Alpha secretase, alpha-secretase, behavioral deficits, biology.protein, Alzheimer, sAPP alpha, business, 030217 neurology & neurosurgery, [SDV.NEU.SC] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Neuroscience
Abstract: International audience; Amyloid β (Aβ) accumulation is considered the main culprit in the pathogenesis of Alzheimer’s disease (AD). Recent studies suggest that decreasing Aβ production at very early stages of AD could be a promising strategy to slow down disease progression. Serotonin 5-HT4 receptor activation stimulates α-cleavage of the amyloid precursor protein (APP), leading to the release of the soluble and neurotrophic sAPPα fragment and thus precluding Aβ formation. Using the 5XFAD mouse model of AD that shows accelerated Aβ deposition, we investigated the effect of chronic treatments (treatment onset at different ages and different durations) with the 5-HT4 receptor agonist RS 67333 during the asymptomatic phase of the disease. Chronic administration of RS 67333 decreased concomitantly the number of amyloid plaques and the level of Aβ species. Reduction of Aβ levels was accompanied by a striking decrease in hippocampal astrogliosis and microgliosis. RS 67333 also transiently increased sAPPα concentration in the cerebrospinal fluid and brain. Moreover, a specific 5-HT4 receptor antagonist (RS 39604) prevented the RS 67333-mediated reduction of the amyloid pathology. Finally, the novel object recognition test deficits of 5XFAD mice were reversed by chronic treatment with RS 67333. Collectively, these results strongly highlight this 5-HT4 receptor agonist as a promising disease modifying-agent for AD.
Published: 2013
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33. Adhesion properties of wheat-based particles

Author: Matthieu George, M. Ramonda, A. Duri, M. Saad, Emmanuelle Gastaldi, Bernard Cuq, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Ctr Technol Montpellier, Université Montpellier 2 - Sciences et Techniques (UM2), Languedoc Roussillon Region through the 'Energie, Poudre, Interface' Chercheur d'Avenir project, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Subjects: Materials science, 030309 nutrition & dietetics, Starch, [SDV]Life Sciences [q-bio], Nanotechnology, Adhesion force, Biochemistry, Endosperm, Surface tension, 03 medical and health sciences, chemistry.chemical_compound, 0404 agricultural biotechnology, Endosperm components (starch, Arabinoxylan, Composite material, Chemical composition, 0303 health sciences, Force spectroscopy, food and beverages, 04 agricultural and veterinary sciences, Adhesion, arabinoxylan), 040401 food science, chemistry, Atomic force microscopy (AFM), Wheat, Particle, protein, Food Science
Abstract: Specific AFM analytical methods were tested to the surface evaluation of wheat powders at micrometric scale in dry conditions. The objective was to evaluate the adhesion properties of particles based on the main wheat endosperm components (starch, protein, and arabinoxylan) that are characterized by large diversity in terms of chemical composition and particle characteristics. Experiments were conducted by using the force spectroscopy mode to determine interactions generated by the surfaces of particles that were glued on AFM tips, and two reference flat and smooth surfaces, a glass and a polysine slide. Particular care has been devoted to characterize the surfaces in contact since local contact radius is a key parameter in the interpretation of the interactions. The statistical analysis of the normalized adhesion forces have shown the sensitivity of the AFM technique for hydrophobic wheat components and their correlation with the surface tension of the particles.
Published: 2013
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34. What Scientific Issues in Life Cycle Assessment Applied to Waste and Biomass Valorization? Editorial

Author: Lynda Aissani, Eva Risch, Véronique Bellon-Maurel, Guillaume Junqua, Philippe Roux, Laurent Lardon, Eléonore Loiseau, Cécile Bessou, Centre de Montpellier [IRSTEA], Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Gestion environnementale et traitement biologique des déchets (UR GERE), Performance des systèmes de culture des plantes pérennes (Cirad-Persyst-UPR 34 Système de pérennes), Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA), Laboratoire de Génie de l'Environnement Industriel (LGEI), IMT - MINES ALES (IMT - MINES ALES), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Languedoc Roussillon region, Interreg Sudoe, ELSA Group, EcoSD network, Performance des systèmes de culture des plantes pérennes (UPR Système de pérennes), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Pôle ELSA, Environmental Life Cycle and Sustainability Assessment (ELSA), Université européenne de Bretagne - European University of Brittany (UEB), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), AgroParisTech, and Interreg Sudoe (Ecotech-Sudoe project)
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Engineering, Environmental Engineering, Complex system, [SDV]Life Sciences [q-bio], 020209 energy, Allocation, Decision, Biomass, 02 engineering and technology, 010501 environmental sciences, Life Cycle Assessment, 01 natural sciences, Field (computer science), 12. Responsible consumption, Unit (housing), 0202 electrical engineering, electronic engineering, information engineering, Production (economics), Waste Management and Disposal, Life-cycle assessment, 0105 earth and related environmental sciences, Scope (project management), Waste management, Renewable Energy, Sustainability and the Environment, business.industry, Impact assessment, [SDE.IE]Environmental Sciences/Environmental Engineering, LCA, Q70 - Traitement des déchets agricoles, Spatialization, Risk analysis (engineering), 13. Climate action, Waste, P01 - Conservation de la nature et ressources foncières, business
Abstract: International audience; Whereas Life Cycle Assessment (LCA) is more and more used for assessing the environmental load of waste management systems and of biomass production and valorization systems, various scientific issues are still to be dealt with. The purpose of this paper is to enlighten these scientific issues and to describe the current attempt to overcome them. The method used has been to go through the steps of the LCA standardized framework (ISO 14040) and to outline at each step the points that could be improved and still deserve research efforts. The various identified issues are: in step 1 (goal and scope), the choice of attributional/consequential modelling, the difficult choice of the functional unit due to the highly multi-functional nature of such systems, the allocation choices and the need for spatial differentiation; in step 2 (inventory), the thorny issue of modelling such complex systems and properly estimating field emissions; in step 3 (impact assessment), the lack of appropriate impacts (such as odours) in current LCA impact categories; in step 4 (interpretation and use), research efforts are needed to understand and facilitate the way actors take over and use LCA multi-criteria results. A transversal issue, i.e. uncertainty characterization and reduction, is also analyzed. These various scientific bottlenecks are currently under study; some are handled by this "Waste and Biomass Valorization" special topic, which includes a selection of papers presented in 2011 at the Ecotech&Tools conference (Montpellier, France).
Published: 2012
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35. Alzheimer culprits: cellular crossroads and interplay

Author: Romain Donneger, Sylvie Claeysen, Maud Cochet, Joël Bockaert, Aline Dumuis, Patrizia Giannoni, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), The authors are supported by grants fromthe CNRS, the INSERM, the French Minister of Research (ANR Blanc 2006, to AD and SC), the FranceAlzheimer association (to SC), the Soroptimist International (French Union, to SC) and the Languedoc Roussillon region ('Chercheuse d'Avenir2012', to SC)., ANR-06-BLAN-0087,GPCR dimers,Molecular and dynamic aspects of G-protein coupled receptor dimers functioning(2006), Giannoni, Patrizia, Programme 'blanc' - Molecular and dynamic aspects of G-protein coupled receptor dimers functioning - - GPCR dimers2006 - ANR-06-BLAN-0087 - BLANC - VALID, and Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: secretase, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Tau protein, Disease, amyloid precursor protein, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, 03 medical and health sciences, Amyloid beta-Protein Precursor, 0302 clinical medicine, Alzheimer Disease, Amyloid precursor protein, medicine, Dementia, Animals, Humans, G protein-coupled receptor, 030304 developmental biology, 0303 health sciences, biology, Mechanism (biology), business.industry, [SCCO.NEUR]Cognitive science/Neuroscience, [SCCO.NEUR] Cognitive science/Neuroscience, Cell Membrane, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, P3 peptide, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Cell Biology, Alzheimer's disease, medicine.disease, 3. Good health, Biochemistry of Alzheimer's disease, amyloidogenic pathway, biology.protein, Amyloid Precursor Protein Secretases, business, Neuroscience, Amyloid precursor protein secretase, 030217 neurology & neurosurgery
Abstract: International audience; Alzheimer's disease (AD) is the primary cause of dementia in the elderly and one of the major health problems worldwide. Since its first description by Alois Alzheimer in 1907, noticeable but insufficient scientific comprehension of this complex pathology have been achieved. All the research that has been pursued takes origin from the identification of the pathological hallmarks in the forms of amyloid-b (Ab) deposits (plaques), and aggregated hyperphosphorylated tau protein filaments (named neurofibrillary tangles). Since this discovery, many hypotheses have been proposed to explain the origin of the pathology. The "amyloid cascade hypothesis" is the most accredited theory. The mechanism suggested to be one of the initial causes of AD is an imbalance between the production and the clearance of Ab peptides. Therefore, Amyloid Precursor Protein (APP) synthesis, trafficking and metabolism producing either the toxic Ab peptide via the amyloidogenic pathway or the sAPPa fragment via the non amyloidogenic pathway have become appealing subjects of study. Being able to reduce the formation of the toxic Ab peptides is obviously an immediate approach in the trial to prevent AD. The following review summarizes the most relevant discoveries in the field of the last decades.
Published: 2012
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36. Modelling adaptive management of intercropping in vineyards to satisfy agronomic and environmental performances under Mediterranean climate

Author: Nakié Paré, Anne Biarnès, Roger Martin-Clouaire, Christian Gary, Jean-Pierre Rellier, Aude Ripoche, Fonctionnement et conduite des Systèmes de culture Tropicaux et Méditerranéens, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Unité de Biométrie et Intelligence Artificielle (UBIA), Institut National de la Recherche Agronomique (INRA), Laboratoire d'étude des Interactions Sol - Agrosystème - Hydrosystème (UMR LISAH), Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), This work was carried out with the financial support of the Agence Nationale de la Recherche - (ANR, French national research agency) under the Programme Agriculture et Developpement Durable, ANR-05-PADD-010, GeDuQuE project. The first author received a fellowship from INRA and Languedoc-Roussillon region (France). We thank Gary Burkhart for the linguistic support and the reviewers for their very detailed and helpful comments to improve the manuscript., and ANR-05-PADD-0010,GeDuQue,Innovations agro-écologiques et organisationnelles pour une Gestion Durable de la Qualité de l'Eau dans des régions de monoculture à forts niveaux d'intrants phytosanitaires(2005)
Subjects: 0106 biological sciences, Mediterranean climate, climate variability, Design management, Computer science, F08 - Systèmes et modes de culture, engineering, [SDV]Life Sciences [q-bio], computer science, Agricultural engineering, Facteur climatique, 01 natural sciences, Relation plante eau, Ecosystem services, interdisciplinary applications, [MATH]Mathematics [math], Cover crop, Adaptive management, 2. Zero hunger, biology, Agroforestry, U10 - Informatique, mathématiques et statistiques, Ecological Modeling, Soil surface management, Intercropping, 04 agricultural and veterinary sciences, flexibility, environmental sciences and ecology, [SDE]Environmental Sciences, système d'aide à la décision, grapevine water status, adaptive management, Environmental Engineering, cover crop, Modelling, Culture intercalaire, modelling, Grapevine water status, pluie méditerranéenne, [INFO]Computer Science [cs], Agricultural productivity, Climate variability, Flexibility (engineering), Modèle de simulation, 15. Life on land, biology.organism_classification, soil surface management, 13. Climate action, Vitis vinifera, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Flexibility, ecosystem services, Software, 010606 plant biology & botany, cultures de couverture
Abstract: International audience; In the Mediterranean area, rainfed viticulture is exposed to irregular rainfall distribution. The impacts on production and environment can be mitigated by appropriate management practices like, for instance, the introduction of cover crop in the inter-rows in vineyards. This paper presents the VERDI simulation model created to study various adaptive intercrop management strategies at field scale. The purpose is to design management strategies that are responsive to the water status of the biophysical system (soil - grapevine - intercrop) and the past and current climatic conditions. VERDI realistically reproduces the dynamic interactions between the biophysical system and the decision system in varying Mediterranean rain regime. The decision system works as an interpreter of a management strategy, defined as a set of soil surface management activities (e.g. mechanical weeding of the intercrop) that are linked by temporal constraints (e.g. sequencing, synchronisation) and organisational or programmatic specifications (e.g. iteration). The adaptive capabilities of the strategies are distinguished according to the different sources of flexibility to be exploited at operational, tactical, and strategic levels. A simulation study is reported that involves more or less flexible strategies under different climate scenarios. The simulation results proved that, in case of severe drought, the most flexible strategy yields the best trade-off between agricultural production and environmental services over the years.
Published: 2011
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37. A spatial stochastic algorithm to reconstruct artificial drainage networks from incomplete network delineations

Author: Jean-Stéphane Bailly, Philippe Lagacherie, Florent Levavasseur, Territoires, Environnement, Télédétection et Information Spatiale (UMR TETIS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-AgroParisTech-Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF), Laboratoire d'étude des Interactions Sol - Agrosystème - Hydrosystème (UMR LISAH), Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), The authors are grateful to the INSU-ECCO-PNRH French national program in hydrology for its general support of this study, which is included in the MOBHYDIC project. They are also grateful to the Languedoc-Roussillon region for the funding of this study through a Ph.D program., Laboratoire d'étude des interactions entre sols, agrosystèmes et hydrosystèmes (LISAH), and Institut National de la Recherche Agronomique (INRA)
Subjects: 010504 meteorology & atmospheric sciences, RESEAU DE DRAINAGE, 0207 environmental engineering, 02 engineering and technology, Management, Monitoring, Policy and Law, Network topology, MAPPING, 01 natural sciences, UNCERTAINTIES, CANAL, GRAPHS, REMOTE SENSING, RANDOM WALKS, INCERTITUDE, DITCHES, Lattice (order), Drainage network, Computers in Earth Sciences, Drainage, 020701 environmental engineering, TELEDETECTION, GRAPHIQUE, CARTOGRAPHIE, 0105 earth and related environmental sciences, Earth-Surface Processes, Random graph, Global and Planetary Change, Data processing, CHANNELS, Reconstruction method, CALCUL SCIENTIFIQUE, Geography, RESEAU HYDROGRAPHIQUE, Simulated annealing, SIMULATION, [SDE]Environmental Sciences, SIMULATED ANNEALING, Algorithm
Abstract: Contact: bailly@teledetection.fr, levavasseur@supagro.inra.fr, lagacherie@supagro.inra.fr; A spatial stochastic algorithm that aims to reconstruct an entire artificial drainage network of a cultivated landscape from disconnected reaches of the network is proposed here. This algorithm uses random network initialisation and a simulated annealing algorithm, both of which are based on random pruning or branching processes, to converge the multi-objective properties of the networks; the reconstructed networks are directed tree graphs, conform to a given cumulative length and maximise the proportion of reconnected reaches. This algorithm runs within a directed plot boundaries lattice, with the direction governed by elevation. The proposed algorithm was applied to a 2.6-km2 catchment of a Languedocian vineyard in the south of France. The 24-km-long reconstructed networks maximised the reconnection of the reaches obtained either from a hydrographic database or remote sensing data processing. The distribution of the reconstructed networks compared to the actual networks was determined using specific topographical and topological metrics on the networks. The results show that adding data on disconnected reaches to constrain reconstruction, while increasing the accuracy of the reconstructed network topology, also adds biases to the geometry and topography of the reconstructed network. This network reconstruction method allows the mapping of uncertainties in the representation while integrating most of the available knowledge about the networks, including local data and global characteristics. It also permits the assessment of the benefits of the remote sensing partial detection process in drainage network mapping.
Published: 2011
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38. Genetic mechanisms underlying the methylation level of anthocyanins in grape (Vitis vinifera L.)

Author: Philippe Hugueney, Clotilde Verries, Alexandre Fournier-Level, Agnès Ageorges, Patrice This, Ageorges, Agnes, Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Sciences Pour l'Oenologie (SPO), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), This work was financially supported in part by the European project program FLAVO 2005-51396, by the French Genoplante project, COREGRAPGEN (Trilat 017), by INRA and by the Languedoc-Roussillon region., Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Subjects: 0106 biological sciences, plant sciences, Plant Science, Berry, 01 natural sciences, anthocyanin, Anthocyanins, Hydroxylation, chemistry.chemical_compound, Gene Expression Regulation, Plant, lcsh:Botany, Vitis, Plant Proteins, Genetics, Regulation of gene expression, 0303 health sciences, Vegetal Biology, qtl, Chromosome Mapping, food and beverages, Methylation, pigment concentration, grape, lcsh:QK1-989, oenologie, visual_art, visual_art.visual_art_medium, marqueur moléculaire, Research Article, DNA, Plant, Molecular Sequence Data, Quantitative Trait Loci, Biology, Quantitative trait locus, color, gene expression, protein, enzyme, méthylation des anthocyanes, 03 medical and health sciences, Pigment, vitis vinifera, pigment, méthylationdes anthocyanes, grappe, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, couleur, 030304 developmental biology, Wine, fungi, Methyltransferases, chemistry, Anthocyanin, vigne, Biologie végétale, Transcription Factors, 010606 plant biology & botany
Abstract: Background Plant color variation is due not only to the global pigment concentration but also to the proportion of different types of pigment. Variation in the color spectrum may arise from secondary modifications, such as hydroxylation and methylation, affecting the chromatic properties of pigments. In grapes (Vitis vinifera L.), the level of methylation modifies the stability and reactivity of anthocyanin, which directly influence the color of the berry. Anthocyanin methylation, as a complex trait, is controlled by multiple molecular factors likely to involve multiple regulatory steps. Results In a Syrah × Grenache progeny, two QTLs were detected for variation in level of anthocyanin methylation. The first one, explaining up to 27% of variance, colocalized with a cluster of Myb-type transcription factor genes. The second one, explaining up to 20% of variance, colocalized with a cluster of O-methyltransferase coding genes (AOMT). In a collection of 32 unrelated cultivars, MybA and AOMT expression profiles correlated with the level of methylated anthocyanin. In addition, the newly characterized AOMT2 gene presented two SNPs associated with methylation level. These mutations, probably leading to a structural change of the AOMT2 protein significantly affected the enzyme specific catalytic efficiency for the 3'-O-methylation of delphinidin 3-glucoside. Conclusion We demonstrated that variation in methylated anthocyanin accumulation is susceptible to involve both transcriptional regulation and structural variation. We report here the identification of novel AOMT variants likely to cause methylated anthocyanin variation. The integration of QTL mapping and molecular approaches enabled a better understanding of how variation in gene expression and catalytic efficiency of the resulting enzyme may influence the grape anthocyanin profile.
Published: 2011
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39. The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas

Author: Duperthuy, Marylise, Binesse, Johan, Le Roux, Frederique, Romestand, Bernard, Caro, Audrey, Got, Patrice, Givaudan, Alain, Mazel, Didier, Bachere, Evelyne, Destoumieux Garzon, Delphine, Université Montpellier 2 - Sciences et Techniques (UM2), Ecosystèmes lagunaires : organisation biologique et fonctionnement (ECOLAG), Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Plasticité du Génome Bactérien (PGB), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Génétique et Pathologie (LGP), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Ecologie microbienne des insectes et interactions hôte-pathogène (EMIP), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), This work received financial support from Ifremer and CNRS. It was awarded a 'chercheur(se) d'avenir' grant from the Languedoc-Roussillon region., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Amélioration génétique, du contrôle des performances et de la santé des mollusques marins (AGSAE), and Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
Subjects: MESH: Adhesins, Bacterial, MESH: Vibrio Infections, MESH: Mutation, MESH: Virulence, MESH: Vibrio, MESH: Blood Proteins, Animals, Humans, MESH: Animals, Crassostrea, OmpU, Adhesins, Bacterial, HUITRE CREUSE, Vibrio, MESH: Genetic Complementation Test, MESH: Humans, Virulence, Genetic Complementation Test, MESH: Antimicrobial Cationic Peptides, Blood Proteins, OYSTER, MESH: Crassostrea, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Gene Deletion, Vibrio Infections, Mutation, Gene Deletion, Antimicrobial Cationic Peptides
Abstract: International audience; Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 microM Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. gigas immunity. The resistance to both oyster Cg-Def and Cg-BPI and standard AMPs (polymyxin B, protegrin, human BPI) was dependent on the ompU gene. Indeed, upon ompU inactivation, minimal bactericidal concentrations decreased by up to fourfold. AMP resistance was restored upon ectopic expression of ompU. The susceptibility of bacterial membranes to AMP-induced damages was independent of the ompU-mediated AMP resistance. Besides its role in AMP resistance, ompU proved to be essential for the adherence of V. splendidus LGP32 to fibronectin. Interestingly, in vivo, ompU was identified as a major determinant of V. splendidus pathogenicity in oyster experimental infections. Indeed, the V. splendidus-induced oyster mortalities dropped from 56% to 11% upon ompU mutation (Kaplan-Meier survival curves, P < 0.01). Moreover, in co-infection assays, the ompU mutant was out competed by the wild-type strain with competitive indexes in the range of 0.1-0.2. From this study, ompU is required for virulence of V. splendidus. Contributing to AMP resistance, conferring adhesive properties to V. splendidus, and being essential for in vivo fitness, the OmpU porin appears as an essential effector of the C. gigas/V. splendidus interaction.
Published: 2010
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