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1. The BMDC model, a performant cell‐based test to assess the sensitizing potential and potency of chemicals including pre/pro‐haptens.

Author

Battais, Fabrice, Langonné, Isabelle, Muller, Samuel, Mathiot, Julianne, Coiscaud, Amélie, Audry, Adrien, Remy, Aurélie Martin, Sponne, Isabelle, and Mourot‐Bousquenaud, Mélanie

Abstract

Background: Chemical‐induced allergies at workplace represent a significant occupational health issue. These substances must be properly identified as sensitizers. In previous studies, an original model using mouse bone marrow‐derived dendritic cells (BMDC) was developed for this purpose. Objectives: The aim of this study was to evaluate the predictive capacity of the BMDC model with a large panel of sensitizers (including pre‐ and pro‐haptens) and non‐sensitizers. Methods: The readout from the BMDC model is based on expression levels of six phenotypic markers measured by flow cytometry. Results: The results indicate that 29 of the 37 non‐sensitizers, and 81 of the 86 sensitizers were correctly classified compared to the Local Lymph Node Assay (LLNA). Statistical analysis revealed the BMDC model to have a sensitivity of 94%, a specificity of 78%, and an accuracy of 89%. The EC2 (Effective Concentration) values calculated with this model allow sensitizers to be categorized into four classes: extreme, strong, moderate and weak. Conclusions: These excellent predictive performances show that the BMDC model discriminates between sensitizers and non‐sensitizers with outstanding precision equal to or better than existing validated alternative models. Moreover, this model allows to predict sensitization potency of chemicals. The BMDC test could therefore be proposed as an additional tool to assess the sensitizing potential and potency of chemicals. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Identification of the allergenic sensitizing potential of bisphenol A substitutes used in the industry.

Author

Mourot‐Bousquenaud, Mélanie, Langonné, Isabelle, Buchheit, Maurane, Muller, Samuel, Coiscaud, Amélie, Mathiot, Julianne, Jacquenet, Sandrine, and Battais, Fabrice

Abstract

Background: Bisphenol (BP‐)A is a chemical used in Europe to produce polycarbonate plastics and epoxy resin or as colour developer in thermal paper. Due to its toxicity, BPA presence was restricted by European regulations. Therefore, substitute chemicals are replacing BPA. Objective: To assess the allergenic sensitizing potential of 27 substitutes to BPA used in the industry. Methods: The expression of two costimulatory molecules and six cytokines were analysed by flow cytometry in mouse bone marrow‐derived dendritic cells (BMDCs) exposed to the chemicals. Results: All substances except one induced overexpression of at least one receptor and were thus identified as having allergenic sensitizing potential. Based on the BMDC model, they were classified as extreme (1 out of 27), strong (20 out of 27) and moderate (5 out of 27) sensitizers. BPA was classified as a moderate sensitizer and BPF was the only substitute classified as a non‐sensitizer. The more potent substitutes induced more than 2‐fold secretion of CCL3, CCL4 and/or CCL5 by dendritic cells. Conclusion: Most of the BPA substitutes tested in this study have an allergenic sensitizing potential; 24 of them being more potent than BPA itself. Only BPE, BPF and 2,4‐BPS appeared to be weaker sensitizers than BPA. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Quand la presse locale s’efface

Author

Olivier Trédan, Philippe Gestin, and Joël Langonné

Subjects
organizational change, economic rationalism, regional daily press, management, France, Communication. Mass media, P87-96
Abstract

The authors investigate how organizational changes based on economic rationalism can affect the process of producing hyperlocal information and impact how things work on the periphery of regional daily press newsrooms in France. They identify new ways of disseminating local news on the fringes of established media and at a microlocal scale. Faced with restrictions imposed by management, key actors in the collection of “small news” are sometimes forced to sidestep newspapers in order to continue doing what they like to do: circulate information in rural areas.

Published
2018
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4. Archéologie du copier-coller

Author

Langonné, Joël, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), and ICAR (CNRS, Université Lyon 2, ENS de Lyon)

Subjects
Copier-coller, matérialité de l'information, [SHS.INFO]Humanities and Social Sciences/Library and information sciences, archéologie des médias, typographie
Abstract

International audience; Le copier-coller est un acte simple qui émaille l’ordinaire de nos pratiques numériques. C’est, si l’on peut dire, un micro-acteur, un intermédiaire technique pratique et discret pour parvenir à faire ce que l’on a à faire. Mais parce qu’il est un entre-deux, le copier-coller est aussi une médiation saisie par un nombre indéfini de gens et de choses. Dans une perspective en archéologie des médias, nous verrons que ce copier-coller-là est capable de traduire des enjeux qui ne le concernent pas a priori : depuis ses débuts analogiques (couper-coller) jusqu’à sa démocratisation numérique, en passant par l’un de ses derniers avatars biotechnologiques (CRISPR/Cas9), le copier-coller est un macro-acteur qui transforme autant qu’il transporte.

Published
2023

5. « Le couper-coller à l’interface de la matérialité du journal : que se passe-t-il donc quand la pince du typographe remplace les ciseaux du SR ? »

Author

Langonné, Joël, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), and Medias 19/ENS Lyon

Subjects
[SHS.INFO]Humanities and Social Sciences/Library and information sciences, atelier de composition, Matérialité de l'écrit, typographes, typographie, Journalisme, secrétaires de rédaction
Abstract

International audience; On sait l’importance de la circulation de textes journalistiques dans l’avènement d'une civilisation du journal au 19e siècle. A mesure que l’on saisit les dimensions matérielles cette viralité, il devient possible de repeupler le monde des journaux de l’ensemble des acteurs qui le composaient alors. Le scissors-and-paste journalism et ses acteurs seraient ainsi cardinaux dans les pratiques généralisées de recopiage. Nous souhaiterions, dans notre communication, déplier encore plus ce journalisme aux ciseaux et décrire ce que devient le couper-coller lorsque l’on se place à l’interface de la matérialité du journal : dans l’atelier de composition. A ce moment-là, le couper-coller est traduit, altére, et devient, si l’on peut dire, un « composer-serrer ». Que se passe-t-il quand la pince du typographe prend le relais des ciseaux du secrétaire de rédaction ? A partir d'un corpus de manuels et d’ouvrages consacrés aux mondes de l’imprimerie et/ou à celui des journaux au 19e siècle, nous découvrons le rôle considérable des typographes des journaux dans les pratiques de recopiage des textes médiatiques.

Published
2023

6. A new cytometry-based method reveals an accumulation of Nrf2 in dendritic cells exposed to two respiratory sensitizers

Author

Fabrice Battais, Isabelle Sponne, Samuel Muller, Amélie Coiscaud, Adrien Audry, Isabelle Langonné, Julianne Mathiot, and Brigitte Leininger

Subjects
Chemistry, Communication, Health, Toxicology and Mutagenesis, Respiratory system, Toxicology, Molecular biology, Cytometry
Abstract

The mechanisms underlying chemical respiratory sensitization are incompletely understood. One of the major cell types involved in this pathology are dendritic cells. In this study, the mechanisms of the NRF2–Keap1 pathway were studied using a bone marrow-derived dendritic cell model exposed to two respiratory sensitizers: ammonium hexachloroplatinate (HCP) and ammonium tetrachloroplatinate (ATCP). Expression levels for two Nrf2-regulated genes, hmox1 and srxn1, were analyzed by real time-quantitative polymerase chain reaction. A flow cytometry-based method was also developed to measure intracellular Nrf2 accumulation in dendritic cells following exposure. Exposure to HCP and ATCP increased both hmox1 and srxn1 gene expression, and was associated with accumulation of Nrf2 protein in cells. Overall, these results show that the respiratory sensitizers, in addition to skin sensitizers, can also induced markers associated with NRF2–Keap1 pathway activation in dendritic cells. This study contributes to a better understanding of the adverse outcome of respiratory sensitization.

Published
2021

9. L’impossible « dernier mot ». La maquette du journal : un outil partagé

Author

Joël Langonné

Subjects
Presse quotidienne régionale, Secrétaires de rédaction, Typographe, Maquette, Théorie de l’acteur-réseau., Journalism. The periodical press, etc., PN4699-5650
Abstract

La maquette du journal occupe une place particulière dans le continuum de fabrication de l’information. Elle est située à l’interface de la matérialité du journal entre la salle de rédaction et le secteur du prépresse. Les discours et les prérogatives dont elle est capable de se charger font qu’elle porte en elle un potentiel stratégique organisationnel important. La maquette du journal demeure pourtant un acteur méconnu de la sociologie des médias. Cette étude, qui se situe au carrefour de la sociologie interactionniste et de la théorie de l’acteur-réseau, propose de donner toute sa place à la maquette et à ceux qui la construisent et l’utilisent au quotidien dans le journal. Nous montrons que c’est entre deux acteurs particuliers et leurs manières différenciées d’être à ce qu’ils font que la maquette est susceptible de révéler tout son potentiel : les journalistes secrétaires de rédaction (SR) et les ouvriers typographes. Nous tentons tout d’abord de saisir pourquoi ces trois acteurs (maquette, SR et typographes) sont demeurés invisibles au sein du journal. Il s’agit donc de repenser leur place et leur rôle, de les révéler au prisme d’une grille d’observation renouvelée, agrandie. Nous sommes ensuite en mesure d’observer la manière dont ces trois acteurs peuvent interagir, peuvent s’attacher au sein du processus de fabrication de l’information. Pour cela, nous développons trois exemples issus d’un travail de terrain au sein d’un titre de presse quotidienne régionale (PQR). Il s’agit en fait de comprendre les manières dont les journalistes SR et les ouvriers typographes sont plus ou moins parvenus — selon les époques retenues, et de manière non linéaire — à se saisir de l’objet maquette, pour faire ce qui est à faire — le journal — au plus près de la manière dont ils souhaitent le faire. The newspaper layout has a special place in the continuum of news production. It is located at the interface between conception and materiality - between newsroom and pre-press area. The discourses and prerogatives for which in can be responsible confer it an important strategic organizational potential. And yet, the newspaper layout remains a little-understood element in the sociology of media. This study, which finds itself at the intersection of interactionist sociology and actor-network theory, is dedicated to the layout and those who construct it and use it daily in the newspaper. We show that it is through their differentiated roles that two agents in particular make it possible for the layout to realize its full potential: editorial secretaries (SR) and typographers. We try first to understand why these three actors (the layout, SR and typographers) have remained invisible within the newspaper. It is therefore a question of rethinking their place and role – of renewing and widening the observation matrix. We can then observe how these three actors interact at the core of the news-production process. To do this, we develop three examples from fieldwork carried out in a regional daily newspaper. In fact, it is an attempt to understand the ways in which copy-editing journalists and typographers were more or less able (depending on the eras selected; and in a non-linear way) to seize control of the layout and create what was necessary – the newspaper – as closely as possible to their vision of how it should be done. A diagramação do jornal ocupa um lugar particular no continuum da produção da informação. Ela está situada na interface da materialidade do jornal, entre a redação e o setor de pré-impressão. Os discursos e as prerrogativas sob a responsabilidade da diagramação garantem a ela um importante potencial estratégico e organizacional. Contudo, a diagramação do jornal continua sendo um ator desconhecido do ponto de vista da sociologia da mídia. Este estudo, que se situa na interseção entre a sociologia interacionista e a teoria do ator-rede, dedica-se ao estudo da diagramação e dos atores que trabalham na construção e na utilização dessa prática no cotidiano do jornal. Destacamos dois atores em particular: os copidesques (em francês: secrétaires de rédaction, SR) e os gráficos. Eles possuem maneiras distintas de ser, o que permite à diagramação revelar todo o seu potencial. Num primeiro momento, buscamos entender em que sentido os três atores (diagramação, copidesques e gráficos) permanecem invisíveis no interior do jornal. Trata-se, portanto, de repensar o seu lugar e o seu papel, de revelá-los ao prisma de uma grelha de observação renovada e ampliada. Em seguida, observamos a forma como esses três atores podem interagir, podem se agrupar em torno do processo de produção da informação. Para isso, desenvolvemos três exemplos oriundos de uma pesquisa de campo realizada no interior de um veículo da impressa regional na França. Trata-se de compreender o modo como os copidesques e os gráficos se utilizam – em momentos específicos e de uma forma não linear – do projeto gráfico para darem conta de suas obrigações – a produção do jornal –, mas buscando aproximar esse processo da forma como eles acreditam que isso deve ser feito.

Published
2014

12. Les petits pas de côté des entreprises de la presse régionale

Author

Joël Langonné, Olivier Trédan, Philippe Gestin, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche Humanités et Sociétés (CHUS), Université Catholique de l'Ouest (UCO), Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
information locale, plateformization, local information, small news, [SHS.INFO]Humanities and Social Sciences/Library and information sciences, Regional Daily Press, Presse locale, GAFAM, informations-services, Plateformisation
Abstract

International audience; This proposal aims to discuss the hypothesis of a reconfiguration of the local information market. By focusing our attention on small pieces of information (information-services), we highlighting some socio-economic issues for the regional daily press and, to a different extent, for the GAFAMs and in particular for Google.; Ce travail vise à discuter de l’hypothèse d’une reconfiguration du marché de l’information de grande proximité, sous l’angle particulier des informations-services, tout en soulignant les enjeux socio-économiques pour la presse quotidienne régionale et, dans une autre mesure, pour les GAFAM et notamment pour Google.

Published
2021

16. Contributions de la Conférence Marsouin 2019 sur la société numérique

Author

Joël Langonné and Nicolas Jullien

Subjects
General Computer Science
Abstract

Marsouin a grandi. Depuis sa creation en 2002, le groupement d’interet scientifique breton accueille et soutient les equipes de recherche en sciences sociales qui travaillent aux carrefours entre les outils numeriques, les gens et leurs environnements. Chemin faisant et sans jamais se departir de son caractere interdisciplinaire et inclusif, le groupement est devenu le plus important pole de recherche francais sur les usages et les innovations numeriques. Chaque printemps, Marsouin reunit uni...

Published
2020

19. A flavivirus protein M-derived peptide directly permeabilizes mitochondrial membranes, triggers cell death and reduces human tumor growth in nude mice

Author

Brabant, Magali, Baux, Ludwig, Casimir, Richard, Briand, Jean Paul, Chaloin, Olivier, Porceddu, Mathieu, Buron, Nelly, Chauvier, David, Lassalle, Myriam, Lecoeur, Hervé, Langonné, Alain, Dupont, Sylvie, Déas, Olivier, Brenner, Catherine, Rebouillat, Dominique, Muller, Sylviane, Borgne-Sanchez, Annie, and Jacotot, Etienne

Published
2009
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20. Dossier M@rsouin 2019 : la société numérique

Author

Langonné, Joël, Jullien, Nicolas, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche Humanités et Sociétés (CHUS), Université Catholique de l'Ouest (UCO), Institut de neurophysiopathologie (INP), and Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SHS]Humanities and Social Sciences
Published
2020

26. In vitrodetection of chemical allergens: an optimized assay using mouse bone marrow-derived dendritic cells

Author

Fabrice Battais, Isabelle Langonné, Cécile Huppert, Samuel Muller, and Isabelle Sponne

Subjects
0301 basic medicine, Chemistry, Local lymph node assay, medicine.medical_treatment, Dermatology, Dendritic cell, Molecular biology, In vitro, respiratory tract diseases, 030207 dermatology & venereal diseases, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Cytokine, Surface marker, medicine, Immunology and Allergy, Potency, Bone marrow, After treatment
Abstract

SummaryBackground Identification of the allergenic potency of chemicals is a key step in the safety assessment process. Predictive assays that require no or few animals are needed. Objectives To develop an alternative in vitro mouse bone marrow-derived dendritic cell (BMDC) assay to determine the allergenic potential of chemicals. Methods BMDCs were exposed to well-known allergens and to non-allergenic chemicals. Surface marker expression and cytokine release of BMDCs were analysed after treatment. Results Eleven tested chemicals showed a significant stimulation index (SI) of >1.5 (accuracy, 75%; sensitivity, 69%). The four non-allergens all showed a SI of 1.5 (accuracy, 92%; sensitivity, 89%), whereas only two respiratory allergens showed a significant SI of >1.5 (accuracy, 60%; sensitivity, 33%). Conclusions The results indicate that the BMDC assay could become a reliable test for assessment of the allergenic potential of chemicals. The next step should include the testing of further chemicals, with the aim of integrating this assay into the toolbox of in vitro methods for the evaluation of the allergenic potential of chemicals.

Published
2017

27. A new cytometry-based method reveals an accumulation of Nrf2 in dendritic cells exposed to two respiratory sensitizers.

Author

Audry, Adrien, Mathiot, Julianne, Muller, Samuel, Coiscaud, Amélie, Langonné, Isabelle, Battais, Fabrice, Leininger, Brigitte, and Sponne, Isabelle

Subjects
ALLERGENS, NUCLEAR factor E2 related factor, POLYMERASE chain reaction
Abstract

The mechanisms underlying chemical respiratory sensitization are incompletely understood. One of the major cell types involved in this pathology are dendritic cells. In this study, the mechanisms of the NRF2–Keap1 pathway were studied using a bone marrow-derived dendritic cell model exposed to two respiratory sensitizers: ammonium hexachloroplatinate (HCP) and ammonium tetrachloroplatinate (ATCP). Expression levels for two Nrf2-regulated genes, hmox1 and srxn1, were analyzed by real time-quantitative polymerase chain reaction. A flow cytometry-based method was also developed to measure intracellular Nrf2 accumulation in dendritic cells following exposure. Exposure to HCP and ATCP increased both hmox1 and srxn1 gene expression, and was associated with accumulation of Nrf2 protein in cells. Overall, these results show that the respiratory sensitizers, in addition to skin sensitizers, can also induced markers associated with NRF2–Keap1 pathway activation in dendritic cells. This study contributes to a better understanding of the adverse outcome of respiratory sensitization. [ABSTRACT FROM AUTHOR]

Published
2021
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28. How Typographers and Subeditors Used to Influence Newspaper Content and Materiality

Author

Joël Langonné, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), and Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materiality (auditing), Actor–network theory, [SHS.INFO]Humanities and Social Sciences/Library and information sciences, Media studies, Sociology, ComputingMilieux_MISCELLANEOUS, Newspaper
Abstract

In this chapter, I discuss two unfamiliar actors of the process of information production in the newspaper: typographers and subeditors. I focus on a particular aspect of the continuum of information production: the prepress. Subeditors and typographers made the newspaper together and in their own respective ways. Traces of these collaborations can be found in the newspaper object – and that is what I am going to try to demonstrate here.

Published
2019

30. Les premiers pas de l’informatique dans les rédactions : l’exemple de journalistes amateurs d’informatique au début des années 1980

Author

Joël Langonné, Olivier Tredan, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SHS.INFO]Humanities and Social Sciences/Library and information sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

35. CD200 expression in human cultured bone marrow mesenchymal stem cells is induced by pro‐osteogenic and pro‐inflammatory cues

Author

Philippe Rosset, Audrey Varin, Mamadou Aliou Ba, Frédéric Deschaseaux, Pierre Charbord, Charalampos Pontikoglou, Alain Langonné, and Luc Sensebé

Subjects
Adult, 0301 basic medicine, Stromal cell, Bone Morphogenetic Protein 7, Interleukin-1beta, Primary Cell Culture, Bone Marrow Cells, Core Binding Factor Alpha 1 Subunit, Bone Morphogenetic Protein 4, Biology, Dexamethasone, Phosphates, 03 medical and health sciences, Antigens, CD, medicine, Humans, Cell Lineage, Progenitor cell, mesenchymal stem/stromal cells, Cell Proliferation, Homeodomain Proteins, Osteoblasts, Tumor Necrosis Factor-alpha, Lineage markers, NF‐κB, Mesenchymal stem cell, NF-kappa B, Cell Differentiation, Mesenchymal Stem Cells, Original Articles, differentiation, Cell Biology, cell cultures, Extracellular Matrix, Cell biology, Bone morphogenetic protein 7, 030104 developmental biology, medicine.anatomical_structure, Bone morphogenetic protein 4, Cell culture, Immunology, Molecular Medicine, Original Article, Bone marrow, heterogeneity, Transcription Factors
Abstract

Similar to other adult tissue stem/progenitor cells, bone marrow mesenchymal stem/stromal cells (BM MSCs) exhibit heterogeneity at the phenotypic level and in terms of proliferation and differentiation potential. In this study such a heterogeneity was reflected by the CD200 protein. We thus characterized CD200(pos) cells sorted from whole BM MSC cultures and we investigated the molecular mechanisms regulating CD200 expression. After sorting, measurement of lineage markers showed that the osteoblastic genes RUNX2 and DLX5 were up-regulated in CD200(pos) cells compared to CD200(neg) fraction. At the functional level, CD200(pos) cells were prone to mineralize the extra-cellular matrix in vitro after sole addition of phosphates. In addition, osteogenic cues generated by bone morphogenetic protein 4 (BMP4) or BMP7 strongly induced CD200 expression. These data suggest that CD200 expression is related to commitment/differentiation towards the osteoblastic lineage. Immunohistochemistry of trephine bone marrow biopsies further corroborates the osteoblastic fate of CD200(pos) cells. However, when dexamethasone was used to direct osteogenic differentiation in vitro, CD200 was consistently down-regulated. As dexamethasone has anti-inflammatory properties, we assessed the effects of different immunological stimuli on CD200 expression. The pro-inflammatory cytokines interleukin-1β and tumour necrosis factor-α increased CD200 membrane expression but down-regulated osteoblastic gene expression suggesting an additional regulatory pathway of CD200 expression. Surprisingly, whatever the context, i.e. pro-inflammatory or pro-osteogenic, CD200 expression was down-regulated when nuclear-factor (NF)-κB was inhibited by chemical or adenoviral agents. In conclusion, CD200 expression by cultured BM MSCs can be induced by both osteogenic and pro-inflammatory cytokines through the same pathway: NF-κB.

Published
2016

37. Human Luteinizing Hormone and Chorionic Gonadotropin Display Biased Agonism at the LH and LH/CG Receptors

Author

Riccetti, Laura, Yvinec, Romain, Klett, Danièle, Langonné, Nathalie, Combarnous, Yves, Reiter, Eric, Simoni, Manuela, Casarini, Livio, Ayoub, Mohammed Akli, Università degli Studi di Modena e Reggio Emilia (UNIMORE), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Le Studium - Loire Valley Institute for Advanced Studies, Région Centre ARD2020 Biomédicaments, Associazione Scientifica in Endocrinologia, Andrologia e Metabolismo, LE STUDIUM® Loire Valley Institute for Advanced Studies and AgreenSkills Plus, European Project: 609398,EC:FP7:PEOPLE,FP7-PEOPLE-2013-COFUND,AGREENSKILLSPLUS(2014), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Modena e Reggio Emilia = University of Modena and Reggio Emilia (UNIMORE), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
steroidogenesis, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Science, Molecular Networks (q-bio.MN), récepteur hormonal, beta arrestine, Article, Mice, steroidogénèse, Cyclic AMP, Animals, Humans, chorionic gonadotropin, Quantitative Biology - Molecular Networks, signal intracellulaire, hormonal receptor, Progesterone, gonadotrophine chorionique, Luteinizing Hormone, Receptors, LH, Recombinant Proteins, HEK293 Cells, beta-Arrestin 1, FOS: Biological sciences, Medicine, hormone recombinante, Autre (Sciences du Vivant)
Abstract

Human luteinizing hormone (LH) and chorionic gonadotropin (hCG) have been considered biologically equivalent because of their structural similarities and their binding to the same receptor; the LH/CGR. However, accumulating evidence suggest that LH/CGR differentially responds to the two hormones triggering differential intracellular signaling and steroidogenesis. The mechanistic basis of such differential responses remains mostly unknown. Here, we compared the abilities of recombinant rhLH and rhCG to elicit cAMP, β-arrestin 2 activation, and steroidogenesis in HEK293 cells and mouse Leydig tumor cells (mLTC-1). For this, BRET and FRET technologies were used allowing quantitative analyses of hormone activities in real-time and in living cells. Our data indicate that rhLH and rhCG differentially promote cell responses mediated by LH/CGR revealing interesting divergences in their potencies, efficacies and kinetics: rhCG was more potent than rhLH in both HEK293 and mLTC-1 cells. Interestingly, partial effects of rhLH were found on β-arrestin recruitment and on progesterone production compared to rhCG. Such a link was further supported by knockdown experiments. These pharmacological differences demonstrate that rhLH and rhCG act as natural biased agonists. The discovery of novel mechanisms associated with gonadotropin-specific action may ultimately help improve and personalize assisted reproduction technologies.

Published
2018

38. A logic-based method to build signaling networks and propose experimental plans

Author

Anne Poupon, Christine Froidevaux, Adrien Rougny, Pascale Crépieux, Nathalie Langonné, Eric Reiter, Pauline Gloaguen, Laboratoire de Recherche en Informatique (LRI), Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Bioinformatique (LRI) (BioInfo - LRI), Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Froidevaux, Christine, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Poupon, Anne, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Biological data, Multidisciplinary, Computer science, Kinase, lcsh:R, lcsh:Medicine, Context (language use), computer.software_genre, Article, Variety (cybernetics), Set (abstract data type), 03 medical and health sciences, 030104 developmental biology, lcsh:Q, Data mining, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], lcsh:Science, computer, ComputingMilieux_MISCELLANEOUS, [INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM]
Abstract

With the dramatic increase of the diversity and the sheer quantity of biological data generated, the construction of comprehensive signaling networks that include precise mechanisms cannot be carried out manually anymore. In this context, we propose a logic-based method that allows building large signaling networks automatically. Our method is based on a set of expert rules that make explicit the reasoning made by biologists when interpreting experimental results coming from a wide variety of experiment types. These rules allow formulating all the conclusions that can be inferred from a set of experimental results, and thus building all the possible networks that explain these results. Moreover, given an hypothesis, our system proposes experimental plans to carry out in order to validate or invalidate it. To evaluate the performance of our method, we applied our framework to the reconstruction of the FSHR-induced and the EGFR-induced signaling networks. The FSHR is known to induce the transactivation of the EGFR, but very little is known on the resulting FSH- and EGF-dependent network. We built a single network using data underlying both networks. This leads to a new hypothesis on the activation of MEK by p38MAPK, which we validate experimentally. These preliminary results represent a first step in the demonstration of a cross-talk between these two major MAP kinases pathways.

Published
2018

39. Activation of T cells by dendritic cells exposed to a reference sensitizer Towards a promising model to assess the allergenic potential of chemicals

Author

Fabrice Battais, Isabelle Sponne, Isabelle Langonné, F. Gagnaire, Christophe Paris, Julianne Mathiot, Hakima Abdessadeq, Samuel Muller, Cécile Huppert, Interactions Gènes-Risques environnementaux et Effets sur la Santé (INGRES), Université de Lorraine (UL), Département Toxicologie et biométrologie (TB), Institut national de recherche et de sécurité (Vandoeuvre lès Nancy) (INRS ( Vandoeuvre lès Nancy)), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )

Subjects
0301 basic medicine, Allergy, medicine.medical_treatment, T cells, Platinum Compounds, Dermatology, Lymphocyte Activation, 03 medical and health sciences, Mice, Chlorides, Surface marker, medicine, Immunology and Allergy, Animals, dendritic cells, Alternative methods, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, Mice, Inbred BALB C, Chemistry, fungi, Limiting, Allergens, medicine.disease, Flow Cytometry, 030104 developmental biology, Cytokine, in vitro assay, chemical allergens, Cancer research, Cytokines, Cell response, Cytokine secretion, Female, Biomarkers
Abstract

International audience; Background - Low molecular weight chemicals constitute one of the major causes of occupational allergies. European legislation on chemicals recommends limiting the use of in vivo models for assessing the sensitizing potential of chemicals, and encourages the development of integrated alternative methods. An in vitro mouse model of bone marrow-derived dendritic cells (BMDCs) that showed good accuracy (75%) and sensitivity (69%) has previously been developed to assess the sensitizing potential of chemicals. Objective - To assess the ability of BMDCs to activate T cells (TCs) in vitro. Methods - BMDCs pre-exposed to the reference sensitizer ammonium hexachloroplatinate (AHCP) were co-cultured with different subpopulations of TCs. TC activation was assessed by surface marker expression, proliferation, and cytokine release. Results - The results showed significant activation of TCs co-cultured with dendritic cells pre-exposed to AHCP as evaluated by CD124 expression, proliferation, and cytokine secretion. Moreover, the response of TCs appeared to be Th2-oriented. Naive TCs were shown to be involved in this response, and the removal of regulatory TCs did not improve the cell response. Conclusions - The BMDCs used in this previously developed model appear to have the ability to activate TCs, confirming that the BMDC model represents a reliable assay for assessing the sensitizing potential of chemicals.

Published
2018

40. Travailler contre les rédacteurs du journal : Des appuis partagés entre pupitreurs et typographes

Author

Joël Langonné, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), and Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SHS.INFO]Humanities and Social Sciences/Library and information sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

42. Penser les mondes du journalisme

Author

Fábio Henrique Pereira, Olivier Tredan, Joël Langonné, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), and Langonné, Joël

Subjects
Cultural Studies, 0508 media and communications, Sociology and Political Science, [SHS.INFO]Humanities and Social Sciences/Library and information sciences, Communication, 05 social sciences, 050801 communication & media studies, 16. Peace & justice, [SHS.INFO] Humanities and Social Sciences/Library and information sciences, ComputingMilieux_MISCELLANEOUS
Abstract

Cet article discute de l’interet de la notion interactionniste de monde social pour les etudes de journalisme. L’approche se veut ancree et souple, et le spectre des acteurs consideres dans l’analyse depasse tres largement les seuls professionnels du journalisme. Les apports beckeriens et straussiens s’averent en effet particulierement feconds pour repeupler les mondes du journalisme de l’ensemble des acteurs qui le composent. Il s’agit en fait de proposer une demarche scientifique particuliere, heritiere d’un reseau de recherche interdisciplinaire et international. Cette proposition tend a saisir les transformations incrementales des mondes du journalisme, et observables selon nous a travers trois notions cles que nous pensons de maniere combinee : les carrieres, les conventions et l’attachement.

Published
2018

43. How typographers and editors used to influence newspaper both content and materiality

Author

Joël Langonné, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SHS.INFO]Humanities and Social Sciences/Library and information sciences, ComputingMilieux_MISCELLANEOUS, [SHS.SCIPO]Humanities and Social Sciences/Political science
Abstract

International audience

Published
2017

44. Des carrières entre deux mondes : journalisme et Légion Etrangère

Author

Joël Langonné, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SHS.INFO]Humanities and Social Sciences/Library and information sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

45. The translatome of the FSH receptor reveals tha several FSH-responsive signaling effectors are co-translated in primary rat Sertoli cells

Author

Trefier, Aurélie, Yvinec, Romain, Bourquard, Thomas, Léon-Huaman, Kelly, Musnier, Astrid, Boulo, Thomas, Morales, Julia, Langonné, Nathalie, Guillou, Florian, Reiter, Eric, Poupon, Anne, Crepieux, Pascale, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), UMR 8227, Centre National de la Recherche Scientifique (CNRS), Sorbonne Universités, Université Pierre et Marie Curie (Paris 6), INRA PHASE Department, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU), Université Pierre et Marie Curie - Paris 6 (UPMC), Université François Rabelais (Tours). Tours, FRA. Centre National de la Recherche Scientifique (CNRS), FRA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Archive Ouverte, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Station biologique de Roscoff [Roscoff] (SBR), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
endocrine system, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], fsh receptor, [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], récepteur hormonal, translatome, G protein coupled receptor, récepteur couplé aux protéines G, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, récepteur fsh, GPCR, signalisation, FSH, cellule de sertoli, hormonal receptor, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, hormones, hormone substitutes, and hormone antagonists
Abstract

The translatome of the FSH receptor reveals tha several FSH-responsive signaling effectors are co-translated in primary rat Sertoli cells. 2. Journées du GdR 3606 Repro

Published
2017

46. L’émergence des pure-players hyper-locaux ou la transformation de la chaîne de production de l’information locale

Author

Joël Langonné, Olivier Tredan, Philippe Gestin, Centre de Recherches sur l'Action Politique en Europe (ARENES), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Rennes (UR)-Institut d'Études Politiques [IEP] - Rennes-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SHS.INFO]Humanities and Social Sciences/Library and information sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

47. A Comprehensive View of the β-Arrestinome

Author

Anne Poupon, Luis Serrano, Pascale Crépieux, Christina Kiel, Eric Reiter, Nathalie Langonné-Gallay, Martin Schaefer, Javier Delgado, Thomas Bourquard, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Dynamiques de populations multi-échelles pour des systèmes physiologiques (MUSCA), Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] (MaIAGE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation, Barcelona Institute of Science and Technology (BIST), Universitat Pompeu Fabra [Barcelona] (UPF), Institució Catalana de Recerca i Estudis Avançats (ICREA), Agence Nationale de la Recherche GPCRnet project, Centre National de la Recherche Scientifique, Institut de la Recherche Agronomique PHASE Department, Région Centre, Spanish Ministerio de Economía y Competitividad, Plan Nacional BIO2012-39754, European Fund for Economic and Regional Development, European Project: 613879, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] (MaIAGE), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Universitat Pompeu Fabra [Barcelona], Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] (MaIAGE)

Subjects
0301 basic medicine, biologie des systèmes, protein/protein interaction network, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Hub proteins, biologie computationnelle, Endocrinology, Diabetes and Metabolism, Systems biology, media_common.quotation_subject, G protein coupled receptor, beta arrestine, transduction du signal, Biology, Interactome, hub proteins, 03 medical and health sciences, Endocrinology, G protein-coupled receptors, Cell surface receptor, Hypothesis and Theory, Protein/protein interaction network, Internalization, Receptor, media_common, G protein-coupled receptor, β-arrestins, Signal transducing adaptor protein, récepteur couplé aux protéines G, systems biology, Cell biology, 030104 developmental biology, Intracellular, Autre (Sciences du Vivant)
Abstract

G protein-coupled receptors (GPCRs) are membrane receptors critically involved in sensing the environment and orchestrating physiological processes. As such, they transduce extracellular signals such as hormone, neurotransmitters, ions, and light into an integrated cell response. The intracellular trafficking, internalization, and signaling ability of ligand-activated GPCRs are controlled by arrestins, adaptor proteins that they interact with upon ligand binding. β-arrestins 1 and 2 in particular are now considered as hub proteins assembling multiprotein complexes to regulate receptor fate and transduce diversified cell responses. While more than 400 β-arrestin interaction partners have been identified so far, much remains to be learnt on how discrimination between so many binding partners is accomplished. Here, we gathered the interacting partners of β-arrestins through database mining and manual curation of the literature to map the β-arrestin interactome (β-arrestinome). We discussed several parameters that determine compatible (AND) or mutually exclusive (XOR) binding of β-arrestin interactors, such as structural constraints, intracellular abundance, or binding affinity. This work was funded by the Agence Nationale de la Recherche GPCRnet project, by the Centre National de la Recherche Scientifique, by the Institut de la Recherche Agronomique PHASE Department, by the Région Centre, and by the European Commission (EC) Framework programme (FP) 7 project SynSignal (contract nr. 613879). LS is supported by the Spanish Ministerio de Economía y Competitividad, Plan Nacional BIO2012-39754, and the European Fund for Economic and Regional Development. LS and CK are particularly grateful for the support of the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013-2017” (SEV-2012-0208).

Published
2017
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48. O discurso da Wan-Ifra: exortação, prescrição e desqualificação dos modelos organizacionais da empresa de informação

Author

Joël Langonné and Magali Prodhomme

Subjects
Modelo organizacional, Convergência, lcsh:Journalism. The periodical press, etc, Communication, lcsh:PN4699-5650, Modelo econômico, Empresa de informação, lcsh:P87-96, lcsh:Communication. Mass media
Abstract

Dentre as várias exortações que permearam o campo do jornalismo, nos últimos dez anos, sobre o papel libertador e até mesmo salvador da era digital, destaca-se uma constante: a crítica aos modelos gerenciais e a dialética dos modelos econômicos ligados às empresas de informação delinearam esferas de ação e produziram discursos sem nunca selar seu destino. Esse destino vem sendo marcado há muitos anos por um processo de desempoderamento dos jornalistas em favor de lógicas gerenciais que adotaram amplamente a “convergência” como uma ferramenta de governança. Este trabalho propõe-se a questionar, dentre as inúmeras mediações que instituem a convergência como modelo estruturador, a materialidade discursiva e ideológica da WAN-IFRA. A essa organização internacional de editores de imprensa atribuiu-se a missão de convencer o mundo da imprensa da necessidade de uma transição para a multiplataforma, para a convergência. Este trabalho interrogará a estabilidade e/ou instabilidade do discurso da WAN-IFRA, bem como sua permeabilidade para absorver outros discursos. Ele indicará, por fim, em meio à nebulosa de discursos prescritivos, aqueles reivindicados por parte dos executivos do campo midiático.

Published
2014

49. The WAN-IFRA discourse: advice, application, and disqualification of organisational models in media

Author

Joël Langonné and Magali Prodhomme

Subjects
Dialectic, Materiality (auditing), business.industry, Aside, Communication, Corporate governance, media_common.quotation_subject, lcsh:Journalism. The periodical press, etc, lcsh:PN4699-5650, Public relations, Organisational model, lcsh:P87-96, Newspaper, lcsh:Communication. Mass media, Political science, Information company, Criticism, Economic model, Journalism, Ideology, business, Convergence, media_common
Abstract

Among the multiple exhortations made on the liberating - even saviour-type - role of the digital era over the past ten years in the field of journalism, one constant has remained: criticism of managerial models and dialectic of economic ones in the media which have defined the spheres of action, resulting in these discourses without ever sealing their fate. A fate that for several years now has been marked by a process in which journalists are being cast aside in favour of a managerial standpoint that broadly integrates 'convergence' as a tool of governance. This paper aims to question (as one of many mediations instituting convergence as a structuring model) WAN-IFRA's discursive and ideological materiality. This international organisation of newspapers and news publishers has set its sights on convincing the print media of the necessity to switching to multiformat; to convergence. This work investigates the stability and/or instability of the WAN-IFRA discourse, as well as its ability to absorb other discourses. Lastly, through a cloud of prescriptive discourse it will indicate those discourses enforced by some managers in the media business.

Published
2014

50. Osteoblastic differentiation and potent osteogenicity of three-dimensional hBMSC-BCP particle constructs

Author

Cordonnier, Thomas, Langonné, Alain, Corre, Pierre, Renaud, Audrey, Sensebé, Luc, Rosset, Philippe, Layrolle, Pierre, Sohier, Jérôme, Jehan, Frederic, Regenerating Bone defects using New biomedical Engineering approaches - REBORNE - - EC:FP7:HEALTH2010-01-01 - 2015-06-30 - 241879 - VALID, Physiopathologie des Adaptations Nutritionnelles (PhAN), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN), Laboratoire d'hématopoïèse [Tours], Université Francois Rabelais [Tours], Etablissement Français du Sang Centre-Atlantique (EA3855), EFS, Regenerative Medicine and Skeleton (RMeS), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Département de Stomatologie et chirurgie maxillo-faciale [CHU Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), Etablissement français du sang [Pyrénées-Méditerranée] (EFS Pyrénées-Méditerranée), Départements de chirurgie orthopédique [CHRU Tours], This work was supported by the French National Research Agency (ATOS project) and partially funded by the Directorate-General for Research of the European Commission (Grant No. 241879) through the REBORNE project., European Project: 241879,EC:FP7:HEALTH,FP7-HEALTH-2009-single-stage,REBORNE(2010), Micro et Nanomédecines Biomimétiques, Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL), Theraptosis Research Laboratory, Theraptosis S.A., Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives, Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), STROMALab, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement Français du Sang-Centre National de la Recherche Scientifique (CNRS), Sarcomes osseux et remodelage des tissus calcifiés - Phy-Os [Nantes] (INSERM U1238), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bretagne Loire (UBL)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Equipe de recherche clinique en Odontologie (ERT1051), Université de Nantes (UN), Micro et Nanomédecines Biomimétiques (MINT), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement Français du Sang-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)-Université Francois Rabelais [Tours], and Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Université Francois Rabelais [Tours]

Subjects
Male, three dimensional constructs, biphasic calcium phosphate, Mice, Nude, Real-Time Polymerase Chain Reaction, Mice, Implants, Experimental, Osteogenesis, Animals, Humans, RNA, Messenger, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, bone tissue engineering, ComputingMilieux_MISCELLANEOUS, Aged, Cell Proliferation, Aged, 80 and over, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Osteoblasts, Tissue Scaffolds, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, glass beads, Cell Differentiation, Mesenchymal Stem Cells, Middle Aged, Microspheres, osteogenicity in nude mice, human bone marrow stromal cells, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Female, Hydroxyapatites, osteoblastic differentiation
Abstract

International audience; Bone tissue engineering usually consists of associating osteoprogenitor cells and macroporous scaffolds. This study investigated the in vitro osteoblastic differentiation and resulting in vivo bone formation induced by a different approach that uses particles as substrate for human bone marrow stromal cells (hBMSCs), in order to provide cells with a higher degree of freedom and allow them to synthesize a three-dimensional (3D) environment. Biphasic calcium phosphate (BCP) particles (35 mg, ~175 µm in diameter) were therefore associated with 4 X 10[5] hBMSCs. To discriminate the roles of BCP properties and cell-synthesized 3D environments, inert glass beads (GBs) of similar size were used under the same conditions. In both cases, high cell proliferation and extensive extracellular matrix (ECM) production resulted in the rapid formation of thick cell-synthesized 3D constructs. In vitro, spontaneous osteoblastic differentiation was observed in the 3D constructs at the mRNA and protein levels by monitoring the expression of Runx2, BMP2, ColI, BSP and OCN. The hBMSC–BCP particle constructs implanted in the subcutis of nude mice induced abundant ectopic bone formation after 8 weeks (~35%, n = 5/5). In comparison, only fibrous tissue without bone was observed in the implanted hBMSC–GB constructs (n = 0/5). Furthermore, little bone formation (~3%, n = 5/5) was found in hBMSC–macroporous BCP discs (diameter 8 X 3 mm). This study underlines the lack of correspondence between bone formation and in vitro differentiation assays. Furthermore, these results highlight the importance of using BCP as well as a 3D environment for achieving high bone yield of interest for bone engineering.

Published
2014
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