Search

Your search keyword '"Langmuir, M. E."' showing total 14 results
Start Over Author "Langmuir, M. E."
14 results on '"Langmuir, M. E."'

14. A novel fluorescent GSH-adduct binds to the NMDA receptor.

Author

Shaw CA, Pasqualotto BA, Curry K, Kim SU, LeCompte KA, and Langmuir ME

Subjects
2-Amino-5-phosphonovalerate analogs & derivatives, 2-Amino-5-phosphonovalerate metabolism, Animals, Cells, Cultured, Excitatory Amino Acid Antagonists metabolism, Fetus, Humans, Neuroglia, Neurons, Rats, Rats, Sprague-Dawley, Fluorescent Dyes metabolism, Receptors, N-Methyl-D-Aspartate metabolism, Receptors, Peptide metabolism
Abstract

In an attempt to develop various fluorescent probes to label glutathione (GSH) receptors, we have serendipitously synthesized a probe that binds to and antagonizes the NMDA receptor. Probe 1, a GSH adduct, displaces the competitive NMDA antagonist [3H]-CGP 39653 with a higher affinity than NMDA or cysteine in rat synaptic membranes. In recording experiments from a rat cortical 'wedge' preparation, Probe 1 reversibly blocks both NMDA- and cysteine-induced depolarization. In mixed astrocyte-neuron tissue culture preparations, Probe 1 labels parts of both cell bodies as well as processes. The present data suggest that Probe 1 binds to the NMDA receptor and antagonizes channel function.

Published
1999
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results