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13. Supplemental_material – Supplemental material for Amnion-derived mesenchymal stem cells improve viability of endothelial cells exposed to shear stress in ePTFE grafts

Author

Pfeiffer, Dagmar, Wankhammer, Karin, Stefanitsch, Christina, Hingerl, Kerstin, Huppertz, Berthold, Dohr, Gottfried, Desoye, Gernot, and Lang, Ingrid

Subjects
FOS: Biological sciences, 69999 Biological Sciences not elsewhere classified
Abstract

Supplemental material, Supplemental_material for Amnion-derived mesenchymal stem cells improve viability of endothelial cells exposed to shear stress in ePTFE grafts by Dagmar Pfeiffer, Karin Wankhammer, Christina Stefanitsch, Kerstin Hingerl, Berthold Huppertz, Gottfried Dohr, Gernot Desoye and Ingrid Lang in The International Journal of Artificial Organs

Published
2018
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14. Preeclampsia: Analysis of subcellular fragments released from the placental trophoblast and their effect on adult endothelial cells utilizing a novel flow-system

Author

Kupper, Nadja, primary, Güttler, Jacqueline, additional, Fuchs, Julia, additional, Brislinger, Dagmar, additional, Lang, Ingrid, additional, Mueller, Marc, additional, Glasmacher, Birgit, additional, Wernitznig, Stefan, additional, Kolb-Lenz, Dagmar, additional, Hirschmugl, Birgit, additional, and Huppertz, Berthold, additional

Published
2019
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15. Correction

Author

Brook, Adam, primary, Hoaksey, Annie, additional, Gurung, Rekha, additional, Yoong, Edward E. C., additional, Sneyd, Rosanna, additional, Baynes, Georgia C., additional, Bischof, Helen, additional, Jones, Sarah, additional, Higgins, Lucy E., additional, Jones, Carolyn, additional, Greenwood, Susan L., additional, Jones, Rebecca L., additional, Gram, Magnus, additional, Lang, Ingrid, additional, Desoye, Gernot, additional, Myers, Jenny, additional, Schneider, Henning, additional, Hansson, Stefan R., additional, Crocker, Ian P., additional, and Brownbill, Paul, additional

Published
2019
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19. Cell free hemoglobin in the fetoplacental circulation: a novel cause of fetal growth restriction?

Author

Brook, Adam, primary, Hoaksey, Annie, additional, Gurung, Rekha, additional, Yoong, Edward E. C., additional, Sneyd, Rosanna, additional, Baynes, Georgia C., additional, Bischof, Helen, additional, Jones, Sarah, additional, Higgins, Lucy E., additional, Jones, Carolyn, additional, Greenwood, Susan L., additional, Jones, Rebecca L., additional, Gram, Magnus, additional, Lang, Ingrid, additional, Desoye, Gernot, additional, Myers, Jenny, additional, Schneider, Henning, additional, Hansson, Stefan R., additional, Crocker, Ian P., additional, and Brownbill, Paul, additional

Published
2018
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20. Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds

Author

Ertl, Juliane, primary, Pichlsberger, Melanie, additional, Tuca, Alexandru-Cristian, additional, Wurzer, Paul, additional, Fuchs, Jakob, additional, Geyer, Stefan H., additional, Maurer-Gesek, Barbara, additional, Weninger, Wolfgang J., additional, Pfeiffer, Dagmar, additional, Bubalo, Vladimir, additional, Parvizi, Daryousch, additional, Kamolz, Lars-Peter, additional, and Lang, Ingrid, additional

Published
2018
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21. Endothelial indoleamine 2,3-dioxygenase-1 regulates the placental vascular tone and is deficient in intrauterine growth restriction and pre-eclampsia

Author

Zardoya-Laguardia, Pablo, primary, Blaschitz, Astrid, additional, Hirschmugl, Birgit, additional, Lang, Ingrid, additional, Herzog, Sereina A., additional, Nikitina, Liudmila, additional, Gauster, Martin, additional, Häusler, Martin, additional, Cervar-Zivkovic, Mila, additional, Karpf, Eva, additional, Maghzal, Ghassan J., additional, Stanley, Chris P., additional, Stocker, Roland, additional, Wadsack, Christian, additional, Frank, Saša, additional, and Sedlmayr, Peter, additional

Published
2018
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22. A novel tryptophan-based mechanism regulating the placental vascular tone – potential contribution to intrauterine growth restriction and preeclampsia

Author

Zardoya-Laguardia, Pablo, primary, Blaschitz, Astrid, additional, Hirschmugl, Birgit, additional, Lang, Ingrid, additional, Herzog, Sereina, additional, Nikitina, Liudmila, additional, Gauster, Martin, additional, Häusler, Martin, additional, Cervar-Zivkovic, Mila, additional, Karpf, Eva, additional, Maghzal, Ghassan, additional, Stanley, Chris, additional, Stocker, Roland, additional, Wadsack, Christian, additional, Frank, Sasa, additional, and Sedlmayr, Peter, additional

Published
2017
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24. Comparison of Matrigel and Matriderm as a carrier for human amnion-derived mesenchymal stem cells in wound healing

Author

Tuca, Alexandru-Cristian, primary, Ertl, Juliane, additional, Hingerl, Kerstin, additional, Pichlsberger, Melanie, additional, Fuchs, Jakob, additional, Wurzer, Paul, additional, Pfeiffer, Dagmar, additional, Bubalo, Vladimir, additional, Parvizi, Daryousch, additional, Kamolz, Lars-Peter, additional, and Lang, Ingrid, additional

Published
2016
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26. Phospholipid transfer protein is expressed in cerebrovascular endothelial cells and involved in HDL biogenesis and remodeling at the blood- brain barrier

Author

Chirackal Manavalan, Anil Paul, Kober, Alexandra, Metso, Jari, Lang, Ingrid, Becker, Tatjana, Hasslitzer, Karin, Zandl, Martina, Fanaee-Danesh, Elham, Briesh Pippal, Jyotsna, Sachdev, Vinay, Kratky, Dagmar, Štefulj, Jasminka, Jauhiainen, Matti, and Panzenboeck, Ute

Subjects
A-beta, blood-brain barrier, brain, cholesterol, cholesterol efflux, endothelium, HDL, LXRs, nuclear receptors, pre-beta HDL, lipids (amino acids, peptides, and proteins)
Abstract

Phospholipid transfer protein (PLTP) is a key protein involved in biogenesis and remodeling of plasma HDL. Several neuro-protective properties have been ascribed to HDL. We reported earlier that liver-X receptor (LXR) activation promotes cellular cholesterol efflux and formation of HDL-like particles in an established in vitro model of the blood-brain barrier (BBB) consisting of primary, porcine brain capillary endothelial cells (pBCEC). Here we report PLTP synthesis, regulation, and its key role in HDL metabolism at the BBB. We demonstrate that PLTP is highly expressed and secreted by pBCEC. In a polarized in vitro model mimicking the BBB, pBCEC secreted phospholipid-transfer active PLTP preferentially to the basolateral (brain parenchymal compartment. PLTP expression levels and phospholipid transfer activity were enhanced (up to 2.5-fold) by LXR activation using 24(S)-hydroxycholesterol (a cerebral cholesterol metabolite) or TO901317 (a synthetic LXR agonist). TO901317 administration elevated PLTP activity in BCEC from C57/BL6 mice. Pre-incubation of HDL3 with human plasma-derived, active PLTP resulted in the formation of smaller and larger HDL particles and enhanced the capacity of the generated HDL particles to remove cholesterol from pBCEC by up to 3-fold. Preβ-HDL, detected by two-dimensional crossed immunoelectrophoresis, was generated from HDL3 in pBCEC-derived supernatants and their generation was markedly enhanced (1.9-fold) upon LXR activation. Furthermore, RNA interference-mediated PLTP silencing (up to 75 %) reduced both apoA-I-dependent (67 %) and HDL3-dependent (30 %) cholesterol efflux from pBCEC. Based on these findings, we propose that PLTP is actively involved in lipid transfer, cholesterol efflux, HDL genesis and remodeling at the BBB.

Published
2014

27. A novel tryptophan-based mechanism regulating the tonus of the placental vascular bed is impaired in intrauterine growth restriction and preeclampsia

Author

Zardoya-Laguardia, Pablo, primary, Blaschitz, Astrid, additional, Hirschmugl, Birgit, additional, Lang, Ingrid, additional, Gauster, Martin, additional, Häusler, Martin, additional, Cervar-Zivkovic, Mila, additional, Karpf, Eva, additional, Wadsack, Christian, additional, Frank, Saša, additional, and Sedlmayr, Peter, additional

Published
2016
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31. Placental mesenchymal stromal cells derived from blood vessels or avascular tissues: what is the better choice to support endothelial cell function?

Author

König, Julia, Weiss, Gregor, Rossi, Daniele, Wankhammer, Karin, Reinisch, Andrea, Kinzer, Manuela, Huppertz, Berthold, Pfeiffer, Dagmar, Parolini, Ornella, Lang, Ingrid, Parolini, Ornella (ORCID:0000-0002-5211-6430), König, Julia, Weiss, Gregor, Rossi, Daniele, Wankhammer, Karin, Reinisch, Andrea, Kinzer, Manuela, Huppertz, Berthold, Pfeiffer, Dagmar, Parolini, Ornella, Lang, Ingrid, and Parolini, Ornella (ORCID:0000-0002-5211-6430)

Abstract

Mesenchymal stromal cells (MSCs) are promising tools for therapeutic revascularization of ischemic tissues and for support of vessel formation in engineered tissue constructs. Recently, we could show that avascular-derived MSCs from placental amnion release soluble factors that exhibit survival-enhancing effects on endothelial cells (ECs). We hypothesize that MSCs derived from placental blood vessels might have even more potent angiogenic effects. Therefore, we isolated and characterized MSCs from placental chorionic blood vessels (bv-MSCs) and tested their angiogenic potential in comparison to amnion-derived avascular MSCs (av-MSCs). bv-MSCs express a very similar surface marker profile compared with av-MSCs and could be differentiated toward the adipogenic and osteogenic lineages. bv-MSCs exert immunosuppressive properties on peripheral blood mononuclear cells, suggesting that they are suitable for cell transplantation settings. Conditioned medium (Cdm) from av-MSCs and bv-MSCs significantly enhanced EC viability, whereas only Cdm from bv-MSCs significantly increased EC migration and network formation (Matrigel assay). Angiogenesis array analysis of av- and bv-MSC-Cdm revealed a similar secretion pattern of angiogenic factors, including angiogenin, interleukins-6 and -8, and tissue inhibitors of matrix metalloproteinase-1 and 2. Enzyme-linked immunosorbent assay analysis showed that, in contrast to av-MSCs, bv-MSCs secreted vascular endothelial growth factor. In direct coculture with bv-MSCs, ECs showed a significantly increased formation of vessel-like structures compared with av-MSCs. With regard to therapeutic treatment, bv-MSCs and particularly their Cdm might be valuable to stimulate angiogenesis especially in ischemic tissues. av-MSCs and their Cdm could be beneficial in conditions when it is required to promote the survival and stabilization of blood vessels without the risk of unmeant angiogenesis

Published
2015

32. Filmverbote in Vorarlberg zwischen 1955 und 1989

Author

Lang, Ingrid

Abstract

Diese Arbeit beschäftigt sich mit den Filmverboten in Vorarlberg zwischen 1955 und 1989. Basierend auf einem Lichtspielgesetz, das 1928 in Kraft trat, wurden ab ebendiesem Zeitpunkt Filme für die gesamte Bevölkerung verboten. Ab 1955 wurden diese Verbote im Amtsblatt für Vorarlberg und/oder im Landesgesetzblatt veröffentlicht. Das letzte Filmverbot findet sich 1989, was den Endpunkt des Untersuchungszeitraumes markiert. Nach Bestimmung der Verbotsbegriffe und der Zensur folgt ein historischer Überblick über die Filmzensur in Österreich bis 1955 mit besonderer Berücksichtigung von Vorarlberg. Im Hauptteil dieser Arbeit wird kurz die allgemeine Verbotssituation in Vorarlberg thematisiert. Nach der Frage des Einflusses des Filmes, insbesondere der Darstellung von Gewalt und Sexualität, beschäftigt sich diese Arbeit mit den Filmverboten von 1955 bis 1989, ihrer Veröffentlichung, den Verbotszahlen und bringt sie in Zusammenhang mit Filmtrends. Es werden die Auswirkungen der Verbote auf die Situation der Kinos und der Einfluss neuer Medien wie Fernsehen, Kabel und Video auf die Verbote untersucht. Die Landtagsdebatten über die Abschaffung des Verbotsparagraphen von 1970 und 1983 werden betrachtet und verglichen. Schließlich wird noch kurz auf die Abschaffung dieses Paragraphen eingegangen.

Published
2011
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35. Feto-placental endothelium in pregnancy disease

Author

Desoye, Gernot, primary, Lang, Ingrid, additional, Wadsack, Christian, additional, Scholler, Monika, additional, Panzenboeck, Ute, additional, Gauster, Martin, additional, Lassance, Luciana, additional, Tokic, Silvija, additional, Loegl, Jelena, additional, Huppertz, Berthold, additional, Nussbaumer, Erika, additional, and Hiden, Ursula, additional

Published
2015
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37. Placental Mesenchymal Stromal Cells Derived from Blood Vessels or Avascular Tissues : What Is the Better Choice to Support Endothelial Cell Function?

Author

König, Julia, Weiss, Gregor, Rossi, Daniele, Wankhammer, Karin, Reinisch, Andreas, Kinzer, Manuela, Huppertz, Berthold, Pfeiffer, Dagmar, Parolini, Ornella, Lang, Ingrid, König, Julia, Weiss, Gregor, Rossi, Daniele, Wankhammer, Karin, Reinisch, Andreas, Kinzer, Manuela, Huppertz, Berthold, Pfeiffer, Dagmar, Parolini, Ornella, and Lang, Ingrid

Abstract

Mesenchymal stromal cells (MSCs) are promising tools for therapeutic revascularization of ischemic tissues and for support of vessel formation in engineered tissue constructs. Recently, we could show that avascular-derived MSCs from placental amnion release soluble factors that exhibit survival-enhancing effects on endothelial cells (ECs). We hypothesize that MSCs derived from placental blood vessels might have even more potent angiogenic effects. Therefore, we isolated and characterized MSCs from placental chorionic blood vessels (bv-MSCs) and tested their angiogenic potential in comparison to amnion-derived avascular MSCs (av-MSCs). bv-MSCs express a very similar surface marker profile compared with av-MSCs and could be differentiated toward the adipogenic and osteogenic lineages. bv-MSCs exert immunosuppressive properties on peripheral blood mononuclear cells, suggesting that they are suitable for cell transplantation settings. Conditioned medium (Cdm) from av-MSCs and bv-MSCs significantly enhanced EC viability, whereas only Cdm from bv-MSCs significantly increased EC migration and network formation (Matrigel assay). Angiogenesis array analysis of av- and bv-MSC-Cdm revealed a similar secretion pattern of angiogenic factors, including angiogenin, interleukins-6 and -8, and tissue inhibitors of matrix metalloproteinase-1 and 2. Enzyme-linked immunosorbent assay analysis showed that, in contrast to av-MSCs, bv-MSCs secreted vascular endothelial growth factor. In direct coculture with bv-MSCs, ECs showed a significantly increased formation of vessel-like structures compared with av-MSCs. With regard to therapeutic treatment, bv-MSCs and particularly their Cdm might be valuable to stimulate angiogenesis especially in ischemic tissues. av-MSCs and their Cdm could be beneficial in conditions when it is required to promote the survival and stabilization of blood vessels without the risk of unmeant angiogenesis.

Published
2014
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41. Phospholipid Transfer Protein Is Expressed in Cerebrovascular Endothelial Cells and Involved in High Density Lipoprotein Biogenesis and Remodeling at the Blood-Brain Barrier

Author

Chirackal Manavalan, Anil Paul, primary, Kober, Alexandra, additional, Metso, Jari, additional, Lang, Ingrid, additional, Becker, Tatjana, additional, Hasslitzer, Karin, additional, Zandl, Martina, additional, Fanaee-Danesh, Elham, additional, Pippal, Jyotsna Brijesh, additional, Sachdev, Vinay, additional, Kratky, Dagmar, additional, Stefulj, Jasminka, additional, Jauhiainen, Matti, additional, and Panzenboeck, Ute, additional

Published
2014
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42. Soluble factors of amnion-derived cells in treatment of inflammatory and fibrotic pathologies

Author

Silini, Antonietta, Parolini, Ornella, Huppertz, Berthold, Lang, Ingrid, Parolini, Ornella (ORCID:0000-0002-5211-6430), Silini, Antonietta, Parolini, Ornella, Huppertz, Berthold, Lang, Ingrid, and Parolini, Ornella (ORCID:0000-0002-5211-6430)

Abstract

Inflammation is a complex defense mechanism characterized by leukocyte migration from the vasculature into damaged tissues and subsequent deposition of extracellular matrix resulting in tissue repair. The inflammatory process is generally categorized into an acute, rapid response, and a persistent but slowly evolving chronic condition, which may progress into inflammatory diseases. An excessive deposition of extracellular matrix leads to overgrowth, hardening, and/or scarring of tissues, defined as fibrosis. The amnion has been used as biomaterial in medicine for over 100 years and has been proven valuable for the treatment of different pathological conditions including wound healing. In light of recent reports, this article will review the effects of the amnion and its cellular components within the inflammatory-fibrotic scenario and the factors described so far that could be involved in these immunomodulatory actions. As proof of principles, we will also discuss pre-clinical and clinical applications of the amnion where advantage has been taken of its anti-inflammatory and anti-fibrotic properties. It is conceivable that the local host environment in which the amnion is placed may have a profound role in influencing the production and function of soluble factors and the shift towards different steps in triggering healing. The healing effect depends on time, dosage, and location of cytokine/growth factor production by the amnion, together with the influence of the host microenvironment. Indeed, determining the specific cascade of events that may define the role of the amnion in a given clinical situation remains a challenge.

Published
2013

44. Amnion-derived mesenchymal stromal cells show angiogenic properties but resist differentiation into mature endothelial cells

Author

König, Julia, Huppertz, Berthold, Desoye, Gernot, Parolini, Ornella, Fröhlich, Julia D, Weiss, Gregor, Dohr, Gottfried, Sedlmayr, Peter, Lang, Ingrid, König, Julia, Huppertz, Berthold, Desoye, Gernot, Parolini, Ornella, Fröhlich, Julia D, Weiss, Gregor, Dohr, Gottfried, Sedlmayr, Peter, and Lang, Ingrid

Abstract

Mesenchymal stromal cells derived from the human amnion (hAMSC) currently play an important role in stem cell research, as they are multipotent cells that can be isolated using noninvasive methods and are immunologically tolerated in vivo. The objective of this study was to evaluate their endothelial differentiation potential with regard to a possible therapeutic use in vascular diseases. hAMSC were isolated from human term placentas and cultured in Dulbecco's modified Eagle's medium (DMEM) (non-induced hAMSC) or endothelial growth medium (EGM-2) (induced hAMSC). Induced hAMSC changed their fibroblast-like toward an endothelial-like morphology, and were able to take up acetylated low-density lipoprotein and form endothelial-like networks in the Matrigel assay. However, they did not express the mature endothelial cell markers von Willebrand factor and vascular endothelial-cadherin. Gene expression analysis revealed that induced hAMSC significantly downregulated pro-angiogenic genes such as tenascin C, Tie-2, vascular endothelial growth factor A (VEGF-A), CD146, and fibroblast growth factor 2 (FGF-2), whereas they significantly upregulated anti-angiogenic genes such as serpinF1, sprouty1, and angioarrestin. Analysis of protein expression confirmed the downregulation of FGF-2 and Tie-2 (27%±8% and 13%±1% of non-induced cells, respectively) and upregulation of the anti-angiogenic protein endostatin (226%±4%). Conditioned media collected from hAMSC enhanced viability of endothelial cells and had a stabilizing effect on endothelial network formation as shown by lactate dehydrogenase and Matrigel assay, respectively. In summary, endothelial induced hAMSC acquired some angiogenic properties but resisted undergoing a complete differentiation into mature endothelial cells by upregulation of anti-angiogenic factors. Nevertheless, they had a survival-enhancing effect on endothelial cells that might be useful in a variety of cell therapy or tissue-engineering approaches., Sponsored byFranz-Lanyar-Foundation, grant no 339349 Medical University of Graz

Published
2012
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