12 results on '"Laneelle MA"'
Search Results
2. Tracking the putative biosynthetic precursors of oxygenated mycolates of Mycobacterium tuberculosis. Structural analysis of fatty acids of a mutant strain deviod of methoxy- and ketomycolates.
- Author
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Dinadayala P, Laval F, Raynaud C, Lemassu A, Laneelle MA, Laneelle G, and Daffe M
- Subjects
- Carbon chemistry, Chromatography, Thin Layer, Magnetic Resonance Spectroscopy, Mass Spectrometry, Models, Chemical, Mutation, Phenotype, Protons, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Fatty Acids metabolism, Mycobacterium tuberculosis metabolism, Mycolic Acids chemistry, Oxygen metabolism
- Abstract
Disruption of the mma4 gene (renamed hma) of Mycobacterium tuberculosis has yielded a mutant strain defective in the synthesis of both keto- and methoxymycolates, with an altered cell-wall permeability to small molecules and a decreased virulence in the mouse model of infection (Dubnau, E., Chan, J., Raynaud, C., Mohan, V. P., Lanéelle, M. A., Yu, K., Quémard, A., Smith, I., and Daffé, M. (2000) Mol. Microbiol. 36, 630-637). Assuming that the mutant would accumulate the putative precursors of the oxygenated mycolates of M. tuberculosis, a detailed structural analysis of mycolates from the hma-inactivated strain was performed using a combination of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, proton NMR spectroscopy, and chemical degradation techniques. These consisted most exclusively of alpha-mycolates, composed of equal amounts of C(76)-C(82) dicyclopropanated (alpha(1)) and of C(77)-C(79) monoethylenic monocyclopropanated (alpha(2)) mycolates, the double bond being located at the "distal" position. In addition, small amounts of cis-epoxymycolates, structurally related to alpha(2)-mycolates, was produced by the mutant strain. Complementation of the hma-inactivated mutant with the wild-type gene resulted in the disappearance of the newly identified mycolates and the production of keto- and methoxymycolates of M. tuberculosis. Introduction of the hma gene in Mycobacterium smegmatis led to the lowering of diethylenic alpha mycolates of the recipient strain and the production of keto- and hydroxymycolates. These data indicate that long-chain ethylenic compounds may be the precursors of the oxygenated mycolates of M. tuberculosis. Because the lack of production of several methyltransferases involved in the biosynthesis of mycolates is known to decrease the virulence of the tubercle bacillus, the identification of the substrates of these enzymes should help in the design of inhibitors of the growth of M. tuberculosis. more...
- Published
- 2003
- Full Text
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3. Characterization of an unusual Mycobacterium: a possible missing link between Mycobacterium marinum and Mycobacterium ulcerans.
- Author
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Chemlal K, Huys G, Laval F, Vincent V, Savage C, Gutierrez C, Laneelle MA, Swings J, Meyers WM, Daffe M, and Portaels F
- Subjects
- Adult, Animals, Bacterial Typing Techniques, Base Sequence, DNA Fingerprinting, DNA Primers genetics, DNA Transposable Elements, DNA, Bacterial genetics, Female, France, Genotype, Humans, Lipids analysis, Molecular Sequence Data, Mycobacterium chemistry, Mycobacterium isolation & purification, Mycobacterium Infections microbiology, Mycobacterium marinum isolation & purification, Mycobacterium ulcerans isolation & purification, Phenotype, Polymerase Chain Reaction, Sequence Homology, Nucleic Acid, Species Specificity, Mycobacterium classification, Mycobacterium genetics, Mycobacterium marinum classification, Mycobacterium marinum genetics, Mycobacterium ulcerans classification, Mycobacterium ulcerans genetics
- Abstract
In an attempt to characterize an unusual mycobacterial isolate from a 44-year-old patient living in France, we applied phenotypic characterizations and various previously described molecular methods for the taxonomic classification of mycobacteria. The results of the investigations were compared to those obtained in a previous study with a set of temporally and geographically diverse Mycobacterium ulcerans (n = 29) and Mycobacterium marinum (n = 29) isolates (K. Chemlal, G. Huys, P.-A. Fonteyne, V. Vincent, A. G. Lopez, L. Rigouts, J. Swings, W. M. Meyers, and F. Portaels, J. Clin. Microbiol. 39:3272-3278, 2001). The isolate, designated ITM 00-1026 (IPP 2000-372), is closely related to M. marinum according to its phenotypic properties, lipid pattern, and partial 16S rRNA sequence. Moreover, fingerprinting by amplified fragment length polymorphism (AFLP) analysis unequivocally classified this strain as a member of the species M. marinum, although it lacked two species-specific AFLP marker bands. However, PCR and restriction fragment length polymorphism analysis based on M. ulcerans-specific insertion sequence IS2404 showed the presence of this element in a low copy number in isolate ITM 00-1026. In conclusion, the designation of this isolate as a transitional species further supports the recent claim by Stinear et al. (T. Stinear, G. Jenkin, P. D. Johnson, and J. K. Davies, J. Bacteriol. 182:6322-6330, 2000) that M. ulcerans represents a relatively recent phylogenetic derivative of M. marinum resulting from the systematic acquisition of foreign DNA fragments. more...
- Published
- 2002
- Full Text
- View/download PDF
4. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier.
- Author
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Camacho LR, Constant P, Raynaud C, Laneelle MA, Triccas JA, Gicquel B, Daffe M, and Guilhot C
- Subjects
- Base Sequence, Cell Membrane Permeability, DNA Primers, Lipids chemistry, Lipids physiology, Molecular Structure, Mutagenesis, Insertional, Mycobacterium tuberculosis metabolism, Reverse Transcriptase Polymerase Chain Reaction, Spectrum Analysis, Cell Wall metabolism, Lipids genetics, Mycobacterium tuberculosis genetics
- Abstract
Among the few characterized genes that have products involved in the pathogenicity of Mycobacterium tuberculosis, the etiological agent of tuberculosis, are those of the phthiocerol dimycocerosate (DIM) locus. Genes involved in biosynthesis of these compounds are grouped on a 50-kilobase fragment of the chromosome containing 13 genes. Analysis of mRNA produced from this 50-kilobase fragment in the wild type strain showed that this region is subdivided into three transcriptional units. Biochemical characterization of five mutants with transposon insertions in this region demonstrated that (i) the complete DIM molecules are synthesized in the cytoplasm of M. tuberculosis before being translocated into the cell wall; (ii) the genes fadD26 and fadD28 are directly involved in their biosynthesis; and (iii) both the drrC and mmpL7 genes are necessary for the proper localization of DIMs. Insertional mutants unable to synthesize or translocate DIMs exhibit higher cell wall permeability and are more sensitive to detergent than the wild type strain, indicating for the first time that, in addition to being important virulence factors, extractable lipids of M. tuberculosis play a role in the cell envelope architecture and permeability. This function may represent one of the molecular mechanisms by which DIMs are involved in the virulence of M. tuberculosis. more...
- Published
- 2001
- Full Text
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5. Overexpression, purification and characterization of Mycobacterium bovis BCG alcohol dehydrogenase.
- Author
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Wilkin JM, Soetaert K, Stélandre M, Buyssens P, Castillo G, Demoulin V, Bottu G, Laneelle MA, Daffe M, and De Bruyn J
- Subjects
- Alcohol Dehydrogenase isolation & purification, Alcohol Dehydrogenase metabolism, Base Sequence, DNA Primers, Kinetics, Phenotype, Phylogeny, Alcohol Dehydrogenase genetics, Mycobacterium bovis enzymology
- Abstract
A previous study of the effect of zinc deprivation on Mycobacterium bovis BCG pointed out the potential importance of an alcohol dehydrogenase for maintaining the hydrophobic character of the cell envelope. In this report, the effect of the overexpression of the M. bovis BCG alcohol dehydrogenase (ADH) in Mycobacterium smegmatis and M. bovis BCG is described. The purification of the enzyme was performed to apparent homogeneity from overexpressing M. bovis BCG cells and its kinetic parameters were determined. The enzyme showed a strong preference for both aromatic and aliphatic aldehydes while the corresponding alcohols were processed 100-1000-fold less efficiently. The best kcat/Km values were found with benzaldehyde > 3-methoxybenzaldehyde > octanal > coniferaldehyde. A phylogenetic analysis clearly revealed that the M. bovis BCG ADH together with the ADHs from Bacillus subtilis and Helicobacter pylori formed a sister group of the class C medium-chain alcohol dehydrogenases, the plant cinnamyl alcohol dehydrogenases (CADs). Comparison of the kinetic properties of our ADH with some related class C enzymes indicated that the mycobacterial enzyme substrate profile resembled that of the CADs involved in plant defence rather than those implicated in lignification. A possible role for the M. bovis BCG ADH in the biosynthesis of the lipids composing the mycobacterial cell envelope is proposed. more...
- Published
- 1999
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6. Lipid composition of leprosy-derived corynebacteria, a distinct group of corynebacteria, and of a reference Corynebacterium.
- Author
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Gailly C, David F, Sandra P, Laneelle MA, and Cocito C
- Subjects
- Cell Wall metabolism, Chemical Fractionation, Chromatography, Thin Layer, Corynebacterium classification, Corynebacterium growth & development, Culture Media, Fatty Acids analysis, Gas Chromatography-Mass Spectrometry, Lipids isolation & purification, Mycolic Acids analysis, Stearic Acids analysis, Corynebacterium chemistry, Leprosy microbiology, Lipids analysis
- Abstract
Leprosy-derived corynebacteria (LDC) are diphtheroid organisms isolated from leprosy patients and previously characterized by DNA and cell wall analysis. Three groups of LDC components of taxonomic value, glycolipids, and phospholipids and cell-wall-bound lipids were analyzed in comparison with those of a reference strain C. hoffmannii (CH). The main CH glycolipid, "cord factor" (trehalose dimycolate), was missing from LDC. Among phospholipids, phosphatidylinositol and phosphatidylglycerol had lowered proportions in LDC, as compared to CH, whereas phosphatidylethanolamine and cardiolipin were absent from both microorganisms. Bound lipids in acidic extracts of delipidated LDC yielded arabinose corynomycolate in lesser quantity with respect to CH. Alkaline hydrolysis of whole cells released fatty acids and mycolic acids, which were analyzed by gas chromatography/mass spectrometry. Reference CH, grown in the absence of serum, yielded C16:0 and C18:1 (major) and C18:0 (minor) fatty acids, as well as C32, C34, and C36 corynomycolic acids. All these components, particularly mycolates, had lowered proportions when this organism was grown in the presence of serum. Dominant LDC components were, in addition to C16:0, C18:0, and CI8:u fatty acids, cholesterol from serum. Very low concentrations of corynomycolic acids with a high degree of unsaturation were found in these organisms, suggesting a dependence of lipid metabolism on growth conditions. The presence in LDC of tuberculostearic acid (C19r:0), a mycobacterial component found in some pathogenic corynebacteria, was carefully explored: Traces of C19r:0 were found in LDC 19 grown in the presence of delipidated serum, but not in LDC 15 nor in C. hoffmannii. Present data, in conjunction with previous studies on DNA and mycolic acids, disclose basic differences in the composition of LDC and conventional corynebacteria. more...
- Published
- 1993
7. Distribution of phthiocerol diester, phenolic mycosides and related compounds in mycobacteria.
- Author
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Daffé M and Laneelle MA
- Subjects
- Fatty Alcohols, Lipids, Mycobacteriaceae metabolism, Mycobacterium bovis analysis, Mycobacterium bovis metabolism, Mycobacterium leprae analysis, Mycobacterium leprae metabolism, Mycobacterium tuberculosis analysis, Mycobacterium tuberculosis metabolism, Mycobacteriaceae analysis, Waxes analysis
- Abstract
Among 28 mycobacterial species studied, only Mycobacterium tuberculosis, M. bovis, M. africanum, M. marinum, M. kansasii, M. gastri and M. ulcerans produced waxes yielding long-chain beta-diol components (called phthiocerol and companions) and polymethyl-branched fatty acids on saponification. The same mycobacterial species also produced diesters of phenol phthiocerol and companions. Fatty acids esterifying these fatty alcohols in M. marinum and M. ulcerans were found to belong to the phthioceranic series (dextrorotatory fatty acids), in contrast to those of the other species (laevorotatory fatty acids called mycocerosic acids), both groups having the same chain length and methyl-branched positions. M. kansasii and M. gastri contained the same waxes with identical structures, as did M. tuberculosis, M. bovis and M. africanum. Neither the type strain of M. tuberculosis, nor that of M. bovis or M. marinum accumulated the strain-specific phenolic glycolipids. more...
- Published
- 1988
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8. Mycolic acid metabolic filiation and location in Mycobacterium aurum and Mycobacterium phlei.
- Author
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Lacave C, Laneelle MA, Daffe M, Montrozier H, and Laneelle G
- Subjects
- Acetates metabolism, Acetic Acid, Carbon Radioisotopes, Kinetics, Mycobacterium growth & development, Mycolic Acids isolation & purification, Species Specificity, Mycobacterium metabolism, Mycobacterium phlei metabolism, Mycolic Acids metabolism
- Abstract
Synchronous cultures of Mycobacterium aurum were used to prove a close relationship between cellular division and active synthesis of mycolic acids (characteristic long-chain 3-hydroxyacids, branched at position 2), confirming previous proposals. Mycolic acid biosynthesis was studied in two species (Mycobacterium phlei and M. aurum) each producing three types of mycolic acids: di-unsatured mycolates, oxomycolates and wax-ester mycolates (ester of dicarboxymycolic acid and 2-icosanol or 2-octadecanol). It was shown that unsaturated mycolates and oxomycolic acids were not directly related, whereas a metabolic filiation was confirmed between oxomycolate and wax ester mycolate: the latter derived from the former by a Baeyer-Villiger oxidation step, as has been proposed on the basis of structural considerations. By observing the labelling of the different mycolate pools in the cell, i.e. the organic-solvent-extractable fraction (essentially containing esters of trehalose and of glycerol) and the cell residue (assumed to be the cell-wall polymers), it was clear that oxomycolates and unsaturated mycolates appeared first in the extractable lipids, then in the wall-linked mycolates while wax-ester mycolates appeared first as wall-linked derivatives. Thus, it is proposed that mycolates could follow separate routes involving differently located enzymes to reach their complex forms either in extractable lipids or in the wall-linked arabino-galactan. more...
- Published
- 1989
- Full Text
- View/download PDF
9. Structural elucidation by field desorption and electron-impact mass spectrometry of the C-mycosides isolated from Mycobacterium smegmatis.
- Author
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Daffe M, Laneelle MA, and Puzo G
- Subjects
- Chemical Phenomena, Chemistry, Electrons, Mass Spectrometry, Glycosides isolation & purification, Mycobacterium analysis
- Abstract
C-mycosides are superficial type-specific glycopeptidolipids of mycobacterial origin. In the present work, we have shown that field desorption mass spectrometry using the cationization method is a useful method for the molecular weight determination of such compounds. Complementary structural information has been obtained by electron-impact mass spectrometry. Combination of both methods has permitted the elucidation of the structure of the C-mycosides of Mycobacterium smegmatis, ATCC 607. This structure is similar to those described elsewhere, but some minor differences are observed in the lipid portion, mainly in the double bond location. more...
- Published
- 1983
- Full Text
- View/download PDF
10. [Comparative study of the lipid composition of seven species of "Micromonospora"].
- Author
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Dassain M, Tiraby G, Laneelle MA, and Asselineau J
- Subjects
- Chemical Phenomena, Chemistry, Chromatography, Thin Layer, Lipids, Micromonospora analysis, Glycolipids analysis, Micromonospora classification, Phospholipids analysis
- Abstract
Analysis of the lipids of seven species of Micromonospora showed the constant presence of five phospholipids and three glycolipids. The phospholipids were identified as cardiolipids, phosphatidylethanolamine, phosphatidyl-inositol, mannosides of phosphatidyl-inositol, and possibly lysocardiolipids. The glycolipids were monoglucosyl-diglycerides, diglucosyl-diglycerides (with a small amount of galactosyl-glucosyl-diglycerides) and esters of fatty acids and trehalose. The simultaneous presence of glucosyl-diglycerides and trehalose esters might be specific of the genus Micromonospora. In the seven species studied, a large amount of the total fatty acids (obtained by direct saponification of the cells) consisted of iso and anteiso fatty acids. Three groups could be distinguished, having respectively iso-C15, iso-C16 and n-C17 fatty acids as the main members. A monounsaturated C17 acid has been identified as heptadec-9,10-enoic acid. more...
- Published
- 1983
11. [STUDIES OF MYCOBACTERIA AND NOCARDIAE. IV. LIPID COMPOSITION OF MYCOBACTERIUM RHODOCROUS, M. PELLEGRINO SP., AND CERTAIN STRAINS OF NOCARDIAE].
- Author
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LANEELLE MA, ASSELINEAU J, and CASTELNUOVO G
- Subjects
- Biochemical Phenomena, Biochemistry, Chemistry Techniques, Analytical, Chloroform, Chromatography, Fatty Acids, Lipids, Mycobacterium, Nocardia, Phospholipids, Research
- Published
- 1965
12. [Etiology of streptothricosis of Chad zebu cattle: nocardiosis or mycobacteriosis? II. Lipid composition].
- Author
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Asselineau J, Laneelle MA, and Chamoiseau G
- Subjects
- Actinomycetales classification, Animals, Cattle, Chad, Lipids analysis, Actinomycosis veterinary, Cattle Diseases microbiology, Mycobacterium Infections veterinary
- Published
- 1969
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