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Research on public policy has been a fast developing fields among social sciences over many decades. In this rapid development, policy analysis reached levels of higher understanding of the policy making process together with the capability of supplying decision makers with reliable and relevant knowledge about urgent economic and social problems. Following Dunn (1981) policy analysis is ���an applied social science discipline which uses multiple methods of inquiry and arguments to produce and transform policy-relevant information that may be utilized in political settings to resolve policy problems.��� And although policy advice is as old as government, modern society faces an increasing complexity that seriously reinforce the decision makers��� need for information. The institutional setting in Italy is ideal for studying public policy, as both the political environment and the labour market undergone dramatic changes over the last 30 years. After almost fifty years of proportional electoral system, in 1991 Italy enter into a period of electoral law reformation. Nevertheless, the debate is ongoing and intense in the attempt to answer the question on how to design an electoral system that is able to express the desired political outcomes in contrast with the rigid structure of the Constitution. Similarly, Italian labour market went through a process of liberalisation since the end of last century when a series of reforms were promoted aiming at fixed-term employment relationships, in the beginning and then moving to targeting open-ended contracts. Alongside, the latest production technologies are characterised by an increased digitalization (Brynjolfsson and McAfee, 2014) that is often included in the political debate with the name of ���Industry 4.0��� and has been related to a significant shift towards the so-called ���smart factory��� of the future. The difficult health crisis, with all its economic and social consequences, has impacted on the country with a wide range of pre-existing structural problems, which it is crucial to maintain the focus on, aside the new paths drawn by the effects of the pandemic. The aim of this thesis is to analyse empirically the Italian institutional setting both in a political competition context and in the occupational structure. In the following chapters, we propose new methods to tackle disputed questions in the literature of political and labour economics: we make use of the traditional Regression Discontinuity Design, as framed by D. S. Lee (2008) with a new randomization tool to address endogeneity; we expand the application of Matrix Completion, a recently developed machine learning techniques to assess deficit in soft skills in the labor market; lastly, we adapt this new Matrix Completion formulation to make predictions on the future trends in the job market and job conditions after the Covid-19 pandemic. The first paper explores the relationship between transfers from central state to political aligned municipalities and the effect of these transfers on local electoral consensus. This study contributes to the empirical literature of the political determinants of spikes in central transfers in pre-electoral periods and of the electoral benefits of pork barrel measures for incumbent politicians. Despite several findings of strong evidence that intergovernmental fiscal transfers rise during election years, in the Italian case researchers investigated little the political incentives that lay behind these increases or the success of these transfers in attracting votes. We focus on the so called swing municipalities, defined as those in which the probability of winning is close to one-half, analysing data of Italian comuni with more than 15 000 inhabitants, in the period 2007-2014. From an empirical perspective, every attempt to estimate the causal impact of political alignment on the amount of federal transfers is clearly complicated by endogeneity issues. Without a credible source of exogenous variation in political alignment, the empirical correlation between alignment and transfers (if any) can be completely driven by socio-economic factors influencing both dimensions. We propose a new model specification to account for the endogeneity issue arising when estimating the causal impact of political alignment on transfers: the unpredicted change in the government occurred in 2011 after the resignation of Silvio Berlusconi and the following appointment of Mario Monti as prime minister. We perform our empirical estimation in two steps: first, we apply the close-race RDD setup (Lee 2008) to assess the impact of political alignment on transfers. Results from the close-race RDD show that aligned municipalities receive more grants, with this effect being stronger before elections. At a second empirical stage, we perform a local linear regression of the reelection probability of the local incumbent on transfers, including the first stage error term to have our coefficient of interest measuring only the effect of politically-driven transfers on electoral outcomes, and we conclude that this probability increases as grants increase. The second paper stems from the observation of the most recent phenomena in the domestic and foreign labour market: technological progress has been associated to a crowdingout of cognitive-skill intensive jobs in favour of jobs requiring soft skills, such as social intelligence, flexibility and creativity. Soft skills can be defined as interpersonal, human, people or behavioural skills necessary for applying technical skills and knowledge in the workplace. The nature of the soft skills make them hardly replaceable by machine work, and Among soft skills, creativity is one of the hardest to define and to codify, therefore, creativity-intensive occupations have been shielded from automation. In our work, we focus on creativity, starting from its definition in order to get significant insights on which occupational profiles in Italy can be considered creative and to explore their dynamics in the labour market. A possible analytical definition of creativity comes from the seminal work of Edward De Bono. According to his pioneering research in the field, lateral thinking is strictly related to creativity and it can be described along four dimensions: 1) fluidity, as the ability of a subject to give the highest possible number of answers to a certain question; 2) flexibility, as the number of categories to which we can bring back these questions; 3) originality: ability of expressing new and innovative ideas; 4) processing: ability of realizing concretely one���s ideas. We apply this definition to the Survey on Occupations (Indagine Campionaria sulle Professioni, ICP hereafter), run by ISTAT and INAPP in 2007 and 2013, the Italian twin of the US O*NET dataset. The Survey on Occupations, in fact, presents a list of skills and competences and workers are asked to identify those they make use of in performing their job. Inside this list, we identify 25 skills associated to creativity and we formulate a Matrix Completion (MC) optimization problem, as discussed theoretically in Mazumder (2010). Matrix Completion is the exercise of reconstructing the missing entries of a matrix, which we generate by obscuring randomly 10%, 25% and 50% of the entries in the columns associated with the creative skills, given a fixed row (occupation). In our analysis, we use a formulation of the problem known as Nuclear Norm Minimization and we solve it with the Soft Impute Algorithm. We conclude our analysis on social skills in our third paper where we analyse the effects of Covid-19 pandemic on soft skills in the context of Italian occupations, operating in about 100 economic sectors. We make use of the information included in the ICP, the Italian O*Net, and we simulate the impact of Covid-19 on those workplace characteristics and working style that were more seriously hit by the lockdown measures and the new sanitary dispositions (physical proximity, face-to-face discussions, working remotely, ecc.). We simulate three possible scenarios based on the intensity of the effects of COVID-19 on some working conditions, such as working from home, keeping physical distance and so on. We then apply matrix completion, a machine learning technique used in recommendation systems, in order to predict the levels of soft skills required for each occupation when working conditions change, as these changes might be persistent in the near future. Professions showing a lower intensity in the use of soft skills, with respect to the predicted one, are exposed to a deficit in their soft-skill endowment, which might ultimately lead to lower productivity or higher unemployment, thus enhancing the negative effects of the pandemic.

Aims: Atrial fibrillation (AF) is the most common type of cardiac arrhythmias, whose incidence is likely to increase with the aging of the population. It is considered a progressive condition, frequently observed as a complication of other cardiovascular disorders. However, recent genetic studies revealed the presence of several mutations and variants linked to AF, findings that define AF as a multifactorial disease. Due to the complex genetics and paucity of models, molecular mechanisms underlying the initiation of AF are still poorly understood. Here we investigate the pathophysiological mechanisms of a familial form of AF, with particular attention to the identification of putative triggering cellular mechanisms, using patient's derived cardiomyocytes (CMs) differentiated from induced pluripotent stem cells (iPSCs). Methods and results: Here we report the clinical case of three siblings with untreatable persistent AF whose whole-exome sequence analysis revealed several mutated genes. To understand the pathophysiology of this multifactorial form of AF we generated three iPSC clones from two of these patients and differentiated these cells towards the cardiac lineage. Electrophysiological characterization of patient-derived CMs (AF-CMs) revealed that they have higher beating rates compared to control (CTRL)-CMs. The analysis showed an increased contribution of the I-f and I-CaL currents. No differences were observed in the repolarizing current I-Kr and in the sarcoplasmic reticulum calcium handling. Paced AF-CMs presented significantly prolonged action potentials and, under stressful conditions, generated both delayed after-depolarizations of bigger amplitude and more ectopic beats than CTRL cells. Conclusions: Our results demonstrate that the common genetic background of the patients induces functional alterations of I-f and I-CaL currents leading to a cardiac substrate more prone to develop arrhythmias under demanding conditions. To our knowledge this is the first report that, using patient-derived CMs differentiated from iPSC, suggests a plausible cellular mechanism underlying this complex familial form of AF.

Aims Atrial fibrillation (AF) is the most common type of cardiac arrhythmias, whose incidence is likely to increase with the aging of the population. It is considered a progressive condition, frequently observed as a complication of other cardiovascular disorders. However, recent genetic studies revealed the presence of several mutations and variants linked to AF, findings that define AF as a multifactorial disease. Due to the complex genetics and paucity of models, molecular mechanisms underlying the initiation of AF are still poorly understood. Here we investigate the pathophysiological mechanisms of a familial form of AF, with particular attention to the identification of putative triggering cellular mechanisms, using patient’s derived cardiomyocytes (CMs) differentiated from induced pluripotent stem cells (iPSCs). Methods and results Here we report the clinical case of three siblings with untreatable persistent AF whose whole-exome sequence analysis revealed several mutated genes. To understand the pathophysiology of this multifactorial form of AF we generated three iPSC clones from two of these patients and differentiated these cells towards the cardiac lineage. Electrophysiological characterization of patient-derived CMs (AF-CMs) revealed that they have higher beating rates compared to control (CTRL)-CMs. The analysis showed an increased contribution of the If and ICaL currents. No differences were observed in the repolarizing current IKr and in the sarcoplasmic reticulum calcium handling. Paced AF-CMs presented significantly prolonged action potentials and, under stressful conditions, generated both delayed after-depolarizations of bigger amplitude and more ectopic beats than CTRL cells. Conclusions Our results demonstrate that the common genetic background of the patients induces functional alterations of If and ICaL currents leading to a cardiac substrate more prone to develop arrhythmias under demanding conditions. To our knowledge this is the first report that, using patient-derived CMs differentiated from iPSC, suggests a plausible cellular mechanism underlying this complex familial form of AF., Graphical Abstract Graphical Abstract

The growing interest in emerging environmental crisis has increased the level of public attention and the willingness to take part in participatory science projects, commonly defined with the term “Citizen Science”. This phenomenon can be recorded globally in many national contexts, with a prevalence in anglophone and more industrialized countries. In the biodiversity sector, the growing demand for public involvement has been declined in many different ways, with solutions aimed at providing cognitive and participatory tools. Among these, one of the best known is the BioBlitz: a 24-hour event held in a specific place with the aim of listing as many living species as possible. This work presents the data collected during the first two BioBlitzes organized by the Maremma Natural History Museum in 2013 and 2014. Both were made in the area of the San Felice Oasis, near the terminal part of the San Leopoldo ditch. During the BioBlitz, data collected attested the presence of 616 different taxonomic entities, of which 507 were identified at the level of species and 22 at the level of subspecies. The rest were identified at higher taxonomic levels. Eleven alien species, one endemic species, 33 species protected by national and international laws and / or directives and 9 species at risk of extinction were identified.

NFIX is a transcription factor involved in the development of various organs among which the brain and the skeletal muscle. In the skeletal muscle, NFIX induces the switch from embryonic to fetal myogenesis and it regulates the regeneration process in the adult mouse. Considering that skeletal and cardiac muscle share several signaling pathways beyond the same mesodermal origin, here we investigated for the first time the expression profile and role of NFIX in the heart. We discovered that Nfix gene is barely expressed at early stages of heart development but its expression increases after birth until postnatal day 7. Histological analysis revealed no apparent morphological alterations Nfix-null hearts when compared to WT littermates. In agreement with these data, no differences in the expression of the sarcomeric genes such as cardiac myosins (Myh6, Myh7, Mlc2v), troponin (cTnI) and of the transcription factor Nkx2.5 were found between WT and Nfix-null mice. In the adult heart, Nfix gene is highly expressed in both atria and ventricles but significantly less expressed in the sinoatrial node (SAN) where the heart rhythm originates. Electrocardiograms recordings in freely moving mice show that Nfix-null mice (523.10�13.25 bpm, n=4) present tachycardia compared to WT mice (466.98�8.42 bpm, n=5). Isolated SAN cardiomyocytes from Nfix-null mice display a higher intrinsic heart rate (577.80�52.80 bpm, n=7) than WT cells (403.80�45.00 bpm, n=7) suggesting that in vivo tachycardia is not caused by a dysregulation of autonomic nervous system. Using RNA interference, we silenced Nfix gene in neonatal rat ventricular cardiomyocytes (NRVCs) and confirmed a +62.2% increase in action potential rate in Nfix-silenced (87.60�7.80 bpm, n=20) compared to scrambled NRVCs (54.00�9.00 bpm, n=19). It is well established the role of pacemaker currents (funny current and calcium currents) in setting action potential rate. We did not find differences in funny current between Nfix-silenced versus control NRVCs and in Nfix-null versus WT SAN cells, but we demonstrated an increase of L-type calcium current in Nfix-silenced NRVCs compared with the control. In line with these functional data, we found a higher expression of Cacna1d-mRNA in Nfix-null SAN than in WT SAN according with a possible increase of CaV1.3 L-type calcium channel density. In conclusion, our data suggest that NFIX acts as negative modulator of the heart rate probably affecting L-type calcium current; however, further analysis are necessary to properly clarify the molecular pathway in which NFIX acts.