8 results on '"Lancheros-Buitrago, Deisy Johana"'
Search Results
2. Seroprevalencia de Leucosis Enzootica Bovina (LEB) y algunos factores de riesgo en bovinos del trópico alto de Boyacá, Colombia
- Author
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González-Bautista, Edgar-Daniel, primary, Bulla-Castañeda, Diana-María, additional, Lancheros-Buitrago, Deisy-Johana, additional, Giraldo-Forero, Julio-César, additional, Castañeda-Sedano, Leneth-Baggerly, additional, and Pulido-Medellín, Martín-Orlando, additional
- Published
- 2024
- Full Text
- View/download PDF
3. Seroprevalencia de Leucosis Enzootica Bovina (LEB) y algunos factores de riesgo en bovinos del trópico alto de Boyacá, Colombia
- Author
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González Bautista, Edgar-Daniel, Ulla, María A., Lancheros Buitrago, Deisy-Johana, Giraldo Forero, Julio César, Castañeda Sedano, Leneth-Baggerly, Pulido Medellín, Martín-Orlando, González Bautista, Edgar-Daniel, Ulla, María A., Lancheros Buitrago, Deisy-Johana, Giraldo Forero, Julio César, Castañeda Sedano, Leneth-Baggerly, and Pulido Medellín, Martín-Orlando
- Abstract
Enzootic Bovine Leukosis (EBL) is a viral disease that affects cattle and has a worldwide distribution, with national prevalences of 13.5-54.6%. Its symptoms can be imperceptible at first, but they manifest in different ways over time depending on the affected organ. The replication mechanism makes the virus virtually impossible to treat, affecting B lymphocytes and monocytes, which predisposes cattle to the incidence of opportunistic pathogenic agents and turns any type of secretion and excretion into a probable contagion factor. The aim of this study was to determine the seroprevalence of EBL and some risk factors in cattle from the high tropic of Boyacá, Colombia. Serological samples from 385 bovine specimens were taken, which were processed using the commercial SERELISA BLV Ab Mono Blocking kit. Prevalence was determined using the Epi Info statistical software, and determining factors were established, calculating the prevalence ratio to identify risk factors. An apparent prevalence (AP) of 19.72% was found. Females were more seropositive (19.94%) than males (14.29%), and the age group from two to four years showed the highest seropositivity for antibodies against the EBL virus (21.21%), followed by individuals aged more than four (20.63%) and animals under two years old (16.6%). Moreover, the Normande breed was the least seropositive (17.4%), while crossbreeds had a higher seroprevalence (22.22%). Although no variable was identified as a risk factor, it is crucial to pay attention to those that showed a significant statistical association, as they may influence the transmission of the virus., La Leucosis Enzoótica Bovina (LEB) es una enfermedad viral que afecta a bovinos y presenta una distribución mundial, con prevalencias de 13.5-54.6 % a nivel nacional. Su sintomatología puede ser imperceptible en un principio, pero con el tiempo se manifiesta de diferentes maneras dependiendo del órgano afectado. El mecanismo de replicación hace que el virus sea prácticamente imposible de tratar, afectando los linfocitos B y los monocitos, lo que predispone al ganado a la incidencia de agentes patógenos oportunistas y hace que cualquier tipo de secreción y excreción se convierta en un probable factor de contagio. El objetivo de este estudio fue determinar la seroprevalencia de LEB y algunos factores de riesgo en bovinos del trópico alto de Boyacá, Colombia. Se tomaron muestras serológicas de 385 ejemplares bovinos, las cuales fueron procesadas utilizando el kit comercial SERELISA BLV Ab Mono Blocking. Se determinó la prevalencia mediante el programa estadístico Epi Info y se establecieron los factores determinantes, calculando la razón de prevalencia para identificar factores de riesgo. Se encontró una prevalencia aparente (PA) del 19.72 %. Las hembras fueron más seropositivas (19.94 %) que los machos (14.29 %), y el grupo etario de 2 a 4 años presentó la mayor seropositividad de anticuerpos contra e virus LEB (21.21%), seguido de los individuos mayores de 4 años (20.63 %) y los animales menores de 2 años (16.6%). Por otra parte, la raza Normando fue la menos seropositiva (17.4%), mientras que los cruces raciales tuvieron mayor seroprevalencia (22.22%). Aunque no se identificó ninguna variable como factor de riesgo, es crucial prestar atención a aquellas que presentaron una asociación estadística significativa, pueden influir en la transmisión del virus.
- Published
- 2024
4. Seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia
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Bulla-Castañeda, Diana María, primary, Lancheros-Buitrago, Deisy Johana, additional, García-Corredor, Diego Jose, additional, Giraldo-Forero, Julio C, additional, and Pulido-Medellin, Martin Orlando, additional
- Published
- 2023
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- View/download PDF
5. Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia)
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Ulla, María A., Ortiz Pineda, Melissa-Camila, Cruz, Nancy Elizabeth, López-Núñez, Henry, Lancheros Buitrago, Deisy-Johana, García, Diego, Pulido, Martín, Giraldo Forero, Julio César, Ulla, María A., Ortiz Pineda, Melissa-Camila, Cruz, Nancy Elizabeth, López-Núñez, Henry, Lancheros Buitrago, Deisy-Johana, García, Diego, Pulido, Martín, and Giraldo Forero, Julio César
- Abstract
Fasciolosis is a worldwide disease caused by the trematode Fasciola hepatica, which affects cattle, sheep, goats, and sometimes humans, causing significant economic and production losses. The objective of this study was to determine the seroprevalence of F. hepatica in sheep from Boavita, Boyacá, Colombia. An observational, descriptive cross-sectional study was carried out with simple random sampling, where the sample size was 297 sheep. The sera were processed with the commercial ELISA BIO K 211-Monoscreen AbELISA F. hepatica kit, following the manufacturer's instructions. The analysis of the results was carried out with the statistical program Epi Info®. A general seroprevalence of 67.34% was determined, where males, sheep older than 3 years, and Creole sheep presented the highest seroprevalence, with 77.78, 83.33, and 64.79%, respectively. A significant statistical association was established between seropositivity to F. hepatica and sheep aged 1 to 3 and individuals older than 3 years. This age group was established as a risk factor for the manifestation of fasciolosis, whereas sheep between 1 to 3 years old were considered as a protective factor. Management variables such as extensive and stake grazing also showed statistical significance, the latter being a protective factor for parasitosis. Our findings show the spread of F. hepatica infections in sheep and confirm the importance of serological methods for the diagnosis of the disease., La fasciolosis es una enfermedad de distribución mundial causada por el trematodo Fasciola hepática, que afecta bovinos, ovinos, caprinos y en ocasiones al ser humano, causando importantes pérdidas económicas y productivas. El objetivo de este estudio fue determinar la seroprevalencia de F. hepatica en ovinos de Boavita, Boyacá, Colombia. Se realizó un estudio observacional y descriptivo de corte (transversal) con muestreo aleatorio simple, en donde el tamaño muestral fue de 297 ovinos. Los sueros fueron procesados con el kit comercial ELISA BIO K 211-Monoscreen AbELISA F. hepatica, siguiendo las instrucciones del fabricante. El análisis de los resultados se realizó con el programa estadístico Epi Info™. Se determinó una seroprevalencia general de 67.34 % en donde los machos, los ovinos mayores de 3 años y las ovejas de raza criolla presentaron la seroprevalencia más alta, con 77.78, 83.33 y 64.79% respectivamente. Se estableció asociación estadística significativa entre la seropositividad a F. hepatica y los ovinos de 1 a 3 años y los individuos mayores 3 años. Este grupo etario se estableció como factor de riesgo para la presentación de fasciolosis, mientras que las ovejas entre 1 y 3 años se consideraron como factor de protección. Las variables de manejo como pastoreo extensivo y de estaca también presentaron significancia estadística, siendo este último un factor de protección para la parasitosis. Nuestros hallazgos evidencian la diseminación de la infección por F. hepatica en ejemplares ovinos y confirman la importancia de los métodos serológicos para el diagnóstico de la enfermedad.
- Published
- 2023
6. Seroprevalence and risk factors associated with the presence of bovine leptospirosis in the municipality of Sotaquirá, Colombia
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Bulla-Castañeda, Diana María, Buitrago, Henry Alexander Lopez, Lancheros-Buitrago, Deisy Johana, Díaz-Anaya, Adriana María, Garcia-Corredor, Diego Jose, Tobón-Torreglosa, Julio Cesar, Ortega, Diego Ortiz, and Pulido-Medellín, Martín Orlando
- Subjects
Cattle ,Leptospira ,Leptospirosis ,Prevalence - Abstract
Background: Bovine leptospirosis is a zoonotic, infectious, and cosmopolitan disease of worldwide distribution, caused by the spirochete Leptospira spp., which has been diagnosed in humans; domestic mammals, such as dogs, sheep, goats, swine, horses and cattle; and wild animals. It is considered a significant cause of economic losses in livestock because it causes infertility, abortion and reduced milk production. Aim: To establish the prevalence and the main risk factors associated with Leptospira spp. in cattle in the municipality of Sotaquirá, Colombia. Methods: An observational, descriptive, cross-sectional study with simple random sampling was carried out. 1,000 cattle of Ayrshire, Holstein, Jersey, Normande, Zebu, and crossbreeds were sampled. Blood samples were taken by coccygeal venipuncture and processed by microscopic agglutination technique; animals were considered positive when titers were ≥1:100. The data obtained were processed with the statistical program EpiInfo®. Results: A general apparent prevalence (AP) of 16% (160/1,000) was established, where the crossbreeds (20.5% AP), the 2–4 years age group (17% AP), and the serovars Leptospira interrogans serogroup Pomona (5.1%) and L. interrogans serogroup Sjroe serovar Hardjo (3.4%) presented the highest seropositivity. The variables barnyard, artificial insemination, and use of certified semen were identified as protective factors against the disease, while diarrhea was considered a risk factor. Conclusion: The prevalence in this study is within the range of those reported at the national level; however, it is essential to establish plans to control and prevent the disease.
- Published
- 2022
7. Serological diagnosis and risk factors associated with bovine paratuberculosis in the municipality of Tuta, Colombia.
- Author
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Cruz-Estupiñan, Sharon Elizabeth, Lancheros-Buitrago, Deisy Johana, María Bulla-Castañeda, Diana, García Corredor, Diego José, and Orlando Pulido-Medellín, Martin
- Subjects
PARATUBERCULOSIS ,SERODIAGNOSIS ,MYCOBACTERIUM avium ,BLOOD sampling ,ENZYME-linked immunosorbent assay - Abstract
Bovine paratuberculosis or Johne's disease is caused by Mycobacterium avium subsp. paratuberculosis (MAP), which affects domestic and wild ruminants around the world. The clinical presentation of MAP infection is characterized by chronic diarrhea unresponsive to treatment. The objective of the present study was to investigate the seroprevalence and risk factors associated with bovine paratuberculosis in cattle herds of Tuta, Boyacá, Colombia. This descriptive cross-sectional study with simple random sampling was performed on 882 blood samples taken from cattle of different racial and age groups. Blood samples were processed using an indirect enzyme-linked immunosorbent assay (PARACHEK ® 2 Kit, Prionics AG, Switzerland). The obtained data were analyzed by the statistical software Epi Info. In this study, a general seroprevalence of 3.1% was found, and seropositivity in females was 3.6%. The highest prevalence of antibodies against MAP was in individuals > 4 years (5%) and the Jersey breed (4.8%). Therefore, the age of over 4 years was identified as a risk factor associated with MAP. Moreover, a statistical association was found between management and biosafety variables, such as pen management (p = 0.012), feeding with concentrate (p = 0.012), and the presence of diarrhea on the farm (p = 0.048). It could be concluded that the disease is present in Tuta, however, considering factors, such as the chronicity of the disease and the diagnostic method used, it is expected that the number of infected animals is much higher than presented in this research. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
8. Equine uterine fluid proteins on fifth day after ovulation
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Lancheros Buitrago, Deisy Johana and Mattos, Rodrigo Costa
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Ovulation ,Proteômica ,Reconhecimento materno da gestação ,Maternal recognition ,mare ,Proteomic ,Ovulação ,Equinos ,Reprodução animal - Abstract
A caracterização do proteoma uterino antes da entrada do embrião no útero, é essencial para conhecer os fatores bioquímicos envolvidos nos eventos fisiológicos da prenhez. O objetivo do estudo foi comparar o perfil proteico do líquido uterino coletado no dia 5 pós-ovulação de éguas cíclicas e inseminadas. Amostras de secreção endometrial foram recuperadas durante o quinto dia pós-ovulação, ao longo de 2 ciclos. O primeiro ciclo constituiu o grupo “cíclico” (n= 10), e no seguinte ciclo, as mesmas éguas foram inseminadas e constituíram o grupo “inseminadas” (n= 10). Todas as amostras foram submetidas à eletroforese bi-dimensional (2D SDS-PAGE). Um total de 107 spots foram visualizados por 2D SDS–PAGE. Três spots com diferencia na abundancia entre as éguas inseminadas e cíclicas e com presença pelo menos em 80% em um dos grupos foram selecionados e identificados. Os spots selecionados foram excisados, digeridos por tripsina e analisados por matrix assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) espectrometria de massa (MS) para identificação proteica. Três proteínas foram identificadas, ceruloplasmina (CP) serotransferrin (ST) e albumina (ALB). As proteínas identificadas neste estudo foram relacionadas com a tolerância imunológica e com o metabolismo do ferro sugerindo que mudanças no perfil proteico durante o dia 5 após à ovulação são provavelmente um mecanismo de sinalização do potencial embrião equino para o sistema imunológico materno para o seu reconhecimento imunológico. Provavelmente, mudanças na abundancia da CP, ALB e TF representam um mecanismo de preparação endometrial para a fixação e desenvolvimento de um potencial embrião equino. Characterization of the uterine proteome before the entry of the conceptus to the uterus is essential to know the factors involved in the physiological events of gestation. The aim of the study was to compare proteomic profile of uterine fluid collected on day 5 post ovulation of cyclic and inseminated mares. Samples of endometrial secretion were recovered over 2 cycles during the fifth day post ovulation. The first cycle constituted the Cyclic group (n = 10) and in the following cycle, the same mares were inseminated and considered as the Inseminated group (n = 10). All the samples were subjected to two-dimensional electrophoresis (2DPAGE). A total of 107 spots were visualized by 2D-PAGE. Three spots with differences in abundance between the inseminated and cyclic mares and with presence in at least 80% in one of the groups were selected and identified. The selected spots were extracted, digested by trypsin and analyzed by matrix assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometry (MS) for protein identification. Three proteins were identified: ceruloplasmin (CP) serotransferrin (TF) and albumin (ALB). The identified proteins in this study were related to iron metabolism and immunological tolerance suggesting that changes in their abundance during the 5 days after ovulation are probably a signaling mechanism of the potential equine conceptus to the maternal immune system for its immunological recognition. Probably changes in abundance of CP, ALB and TF represent a mechanism of endometrial preparation for the maternal recognition, attachment and development of a potential equine embryo.
- Published
- 2019
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