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The ability to read and compose original scientific literature is critical to educating informed citizens, yet may be severely lacking in undergraduate curricula. We developed a linked course offering in fall 2017 consisting of a 16-student group taking an introductory biology course paired with a first-year composition course. The composition course focused specifically on scientific literature composed in the Introduction, Methods, Results, and Discussion (IMRaD) structure. In the composition course, instruction focused on helping students conceptualize and compose proposals, poster presentations, and manuscripts based on their lab activities. We surveyed for prior experience with scientific literature and self-reported reading and writing confidence. Participants in the linked courses showed an improvement in self-reported ability and confidence in writing compared to a control group that did not participate. We assembled a rubric assessing correct IMRaD structure and used it to assess composition before and after the linked courses. Adherence to IMRaD improved significantly for the Methods section. Our experiences indicate that learning communities can be useful pedagogies to provide students with confidence and learning gains that facilitate use of information and communication.

Microorganisms are diverse, minute, simple life-forms that generally cannot be seen by the naked eye and require the use of a microscope to be visualized. They have a great impact on all other life-forms. Their tiny size conceals them from us, engendering misunderstanding and fear due to the diseases caused by only a tiny minority of them. We conceptualized and installed an art exhibition called "Tiny Enormous" with the intent to educate our campus community and correct misconceptions about microorganisms. "Tiny Enormous" utilized a variety of artistic media, including paint, sculpture, video, and preserved plates, to display the diversity and ubiquity of microbes, and a series of infographics to illustrate key concepts and correct misconceptions. We surveyed visitors at the opening and closing receptions to examine their knowledge about, and perceptions and attitudes toward, microorganisms prior to and after visiting the exhibition. Respondents who had viewed "Tiny Enormous" demonstrated better knowledge of microbiological terms and concepts and self-reported increased knowledge about microorganisms compared to those who had not. Perceptions that microorganisms were harmful did not differ between subjects prior to and after visiting "Tiny Enormous," possibly because of the exhibition's information about "superbugs." Our results suggest that artistic representations of microorganisms are effective educational tools for both academic and nonacademic audiences.

Insects are the most abundant and diverse animals on planet Earth and rely on their diverse microbiomes to be so. The insect gut microbiome is vital in the growth and development of many insect species. Trichoplusia ni, the cabbage looper, is a generalist herbivore, but little is known about its microbiome. In this study, a metagenomic analysis of fecal samples was used to determine the effect of diet on the microbiome of T. ni larvae. Larvae were reared on six plant species, the microbiome was sampled from fecal material, and the phyllosphere was sampled from leaves the larvae ate. Bacteria were identified using 16S ribosomal RNA gene sequences, and diversity was quantified. Similar phyla, classes, and families were found in both types of samples, suggesting T. ni larvae primarily obtain their gut microbiome from their diet. However, the gut microbiome of T. ni larvae is not identical to the phyllosphere, particularly in relative abundances, suggesting that other factors in the insect gut environment may further modify the diversity of the microbiome. This study adds to the growing body of evidence about the implications of diet for the insect gut microbiome. Keywords: 16S rRNA gene, Illumina sequencing, metagenomics, Trichoplusia ni, INTRODUCTION Insects are members of the most abundant and diverse animal clade on planet Earth (Basset et al. 2012). The evolutionary success of insects depends partly on their symbiotic relationship [...]

This article discusses the importance of promoting entomology and conservation interests among at-risk children through arthropod workshops. The decline of arthropod populations worldwide poses a threat to ecosystems, and public support for conservation is crucial. At-risk children, who often face educational disadvantages, can become environmental advocates if they are exposed to the importance of arthropods and their ecosystem services. The article presents the results of field testing workshops conducted with at-risk children, showing that their attitudes towards insects and careers in biology can be positively influenced through engagement and education. Further research is needed to explore the long-term effects of these attitude changes and how knowledge and engagement impact participation in conservation activities. [Extracted from the article]

Diverse communities of arthropods and microbes provide humans with essential ecosystem goods and services. Arthropods are the most diverse and abundant macroscopic animals on the planet, and many remain to be discovered. Much less is known about microbial diversity, despite their importance as free-living species and as symbionts. We created "Bugs on Bugs" as an inquiry-based research project in which students investigate both arthropod and microbe diversity by collecting arthropods and culturing their symbionts. "Bugs on Bugs" was developed as a multiple-course project in which students from different disciplines specialize in parts of the project and collaborate in project design and data analysis. We provide instructions for use of "Bugs on Bugs" in active-learning courses, share experiences in which a biodiversity course and a microbiology course completed "Bugs on Bugs" together at our institution, and share suggestions for implementation based on our experiences.

Invasive species, defined as exotic species that reach pest status, are major threats to global biodiversity. Although invasive species can belong to any taxonomic group, general characteristics such as rapid growth and reproduction are shared by many invasive species. "Invented Invaders" is a collaborative activity in which students invent and draw the "perfect" invasive species, made up of a combination of characteristics of actual invasive species in their geographic region. Students then list sustainable ways to control or manage the invented invader. This activity engages students and educates them about a continuing global problem while encouraging creativity and advocacy for the environment.

Fragmented landscapes are a mosaic of ecosystems containing diverse communities of plants and herbivorous arthropods. Plant responses to fire in fire-prone ecosystems are well documented, but less is known about how plant-herbivorous arthropod interactions respond to fires. This study compared the responses of plant communities and their interactions with herbivorous arthropods to fire in a highly fragmented fire-prone glade system. Due to the mosaic landscape of the study site, three habitat types were chosen to delineate communities based on plant species composition, geology, and proximity to each other: small enclosed glade, large open glade, mixed hardwood forest, and pine savanna. From 2016–2019, we observed the interspecific interactions between plant and arthropod communities in the Ketona dolomitic glades of Bibb County in central Alabama. We identified plants to genus or species and recorded evidence of herbivory by seven herbivore guilds of arthropods. We used non-Metric Multidimensional Scaling and Analysis of Similarity to determine patterns of change between the habitats and across years for both plant species and interactions with herbivorous arthropods. Plant communities return to pre-burn species diversity within two years. Plant community composition was grouped strongly by habitat type and year, while interactions with herbivorous arthropods were homogenous across habitats but grouped strongly by year. Tracking herbivorous arthropod guilds using plant association evidence is useful for rapidly and temporally determining overall responses in herbivorous arthropod guild composition. However, it is too coarse to determine changes and responses in herbivorous arthropod guild composition at finer spatial scales. [ABSTRACT FROM AUTHOR]

"Biology Myth-Killers" is an activity designed to identify and correct common misconceptions for high school and college introductory biology courses. Students identify common myths, which double as biology misconceptions, and use appropriate sources to share the "truth" about the myths. This learner-centered activity is a fun and engaging way to correct misconceptions and prepare students to overcome other important misconceptions.

Sequestration of defensive compounds acquired from the environment can be costly to herbivorous insects. The catalpa sphinx, Ceratomia catalpae Boisduval (Lepidoptera: Sphingidae) sequesters the iridoid glycoside catalpol, which it acquires from Catalpa spp. (Lamiales: Bignoniaceae) host plants, at concentrations above those in host plant leaves. Previous research has shown that C. catalpae larvae are avoided by predatory arthropods; however, catalpol sequestration is negatively associated with the larval immune response, suggesting a cost to sequestration. By measuring the mass of C. catalpae larvae, their frass, and their diet, as well as respiration rate and catalpol sequestration, this study aimed to determine whether sequestration is also associated with reduced growth and nutritional efficiency. I found that respiration rate was negatively correlated with the amount of catalpol sequestered and positively correlated with the concentration, or proportion dry mass of catalpol sequestered, and that the amount of catalpol sequestered was positively correlated with three of four measures of nutritional efficiency. Larger larvae that were closer to pupation sequestered very low proportions dry mass of catalpol and had a reduced respiratory rate per unit mass. These results suggest that sequestration has some costs for expenditure of energy, but is positively correlated with several growth and nutrition-related processes. Instead, the immunological cost of sequestration measured in previous studies could be due to direct effects on immune system components. Further research on the potential metabolic costs of chemical defense in other specialist species is recommended. [ABSTRACT FROM AUTHOR]

1. Plant defensive chemistry is predicted to have a more negative effect on generalist herbivores and their parasitoids than on specialist herbivores and their parasitoids. 2. This prediction was examined by comparing the effects of the wild parsnip ( Pastinaca sativa L.) toxin, xanthotoxin, on a generalist herbivore-parasitoid association [the cabbage looper, Trichoplusia ni Hübner, and its polyembryonic parasitoid, Copidosoma floridanum (Ashmead)] and a specialist herbivore-parasitoid association [the parsnip webworm, Depressaria pastinacella (Duponchel), and its polyembryonic parasitoid, Copidosoma sosares (Walker)]. 3. Copidosoma floridanum brood sizes were smaller and experienced lower survivorship when reared in a host feeding on an artificial diet containing a low concentration of xanthotoxin. No T. ni hosts, parasitised or unparasitised, survived on a diet high in xanthotoxin. In contrast, C. sosares brood size and survivorship were unaffected by the presence of low levels of xanthotoxin in the host diet. Copidosoma sosares experienced reduced brood size and survivorship only when its host consumed a diet containing 15 times the level of xanthotoxin as the diet adversely affecting its congener. 4. The differences in response to xanthotoxin exhibited by C. floridanum and C. sosares are explained partly by a differential reduction in host quality and partly by differential exposure to xanthotoxin in host haemolymph. Unlike D. pastinacella, T. ni experienced reduced pupal weight and survivorship and prolonged developmental time on a low-xanthotoxin diet. More xanthotoxin passed unmetabolised into the haemolymph of T. ni than into the haemolymph of D. pastinacella. [ABSTRACT FROM AUTHOR]

Diet of herbivorous insects can influence both the herbivores and their natural enemies. We examined the direct and indirect effects of diet on the interactions between the polyphagous herbivore Trichoplusia ni Hübner (Lepidoptera: Noctuidae) and its polyembryonic parasitoid Copidosoma floridanum Ashmead (Hymenoptera: Encyrtidae). To determine how host plant species and host plant iridoid glycoside content affect host caterpillars and their parasitoids, parasitized and unparasitized T. ni were given leaves of either Plantago lanceolata L., which contains the iridoid glycosides aucubin and catalpol, Plantago major L. (Plantaginaceae), which contains only aucubin, or Taraxacum officinale F.H. Wigg (Asteraceae), which contains neither. Survival of unparasitized T. ni was much lower when fed P. major compared with the other two host plants, whereas pupae were smallest when fed T. officinale and developed most slowly when fed P. lanceolata as larvae. Neither aucubin nor catalpol were detected in intact Plantago-fed T. ni larvae or their hemolymph, and only trace amounts of aucubin were detected in frass, suggesting that these compounds are mostly metabolized in the midgut and are not encountered by the parasitoid. Copidosoma floridanum clutch size was almost doubled when reared from P. lanceolata-fed T. ni compared with T. officinale-fed larvae and tripled compared with P. major-fed larvae, although the percent of parasitoids surviving to adulthood was uniformly high regardless of host diet. The observed variation in C. floridanum fitness among host diets is likely mediated by the effect of the diets on host quality, which in turn may be influenced more by other factors in the host plants than their iridoid glycoside profiles. Interactions between plant metabolites, generalist herbivores like T. ni, and their parasitoids may be predominantly indirect. [ABSTRACT FROM AUTHOR]