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10 results on '"Lamichhane, Aayam"'

2. MUCOSAL IMMUNOLOGY: Innate lymphoid cells regulate intestinal epithelial cell glycosylation

Author

Goto, Yoshiyuki, Obata, Takashi, Kunisawa, Jun, Sato, Shintaro, Ivanov, Ivaylo I., Lamichhane, Aayam, Takeyama, Natsumi, Kamioka, Mariko, Sakamoto, Mitsuo, Matsuki, Takahiro, Setoyama, Hiromi, Imaoka, Akemi, Uematsu, Satoshi, Akira, Shizuo, Domino, Steven E., Kulig, Paulina, Becher, Burkhard, Renauld, Jean-Christophe, Sasakawa, Chihiro, Umesaki, Yoshinori, Benno, Yoshimi, and Kiyono, Hiroshi

Published
2014
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4. Microbe-dependent CD11b+ IgA+ plasma cells mediate robust early-phase intestinal IgA responses in mice

Author

Izumi Ishikawa, Shizuo Akira, Yoshiyuki Goto, Shiori Shikata, Yosuke Kurashima, Morio Higuchi, Yuji Suzuki, Jun Kunisawa, Casandra Panea, Eri Hashimoto, So Tajiri, Ivaylo I. Ivanov, Taichi Wake, Hiroshi Kiyono, Kiyoshi Takeda, Lamichhane Aayam, Masashi Gohda, and Risa Sumiya

Subjects
Immunoglobulin A, Ovalbumin, T cell, Plasma Cells, Administration, Oral, General Physics and Astronomy, Plasma cell, Article, General Biochemistry, Genetics and Molecular Biology, Mice, Peyer's Patches, medicine, Animals, Cell Proliferation, CD11b Antigen, Multidisciplinary, Bacteria, biology, Cell growth, General Chemistry, Molecular biology, Interleukin-10, Intestines, Interleukin 10, medicine.anatomical_structure, Integrin alpha M, Immunology, biology.protein, Immunization, Antibody
Abstract

Intestinal plasma cells predominantly produce immunoglobulin (Ig) A, however, their functional diversity remains poorly characterized. Here we show that murine intestinal IgA plasma cells can be newly classified into two populations on the basis of CD11b expression, which cannot be discriminated by currently known criteria such as general plasma cell markers, B cell origin and T cell dependence. CD11b+ IgA+ plasma cells require the lymphoid structure of Peyer’s patches, produce more IgA than CD11b− IgA+ plasma cells, proliferate vigorously, and require microbial stimulation and IL-10 for their development and maintenance. These features allow CD11b+ IgA+ plasma cells to mediate early-phase antigen-specific intestinal IgA responses induced by oral immunization with protein antigen. These findings reveal the functional diversity of IgA+ plasma cells in the murine intestine., Intestinal plasma cells contribute to the delicate balance between immunity against pathogens and tolerance of intestinal microflora. Kunisawa et al. identify a subpopulation of plasma cells whose proliferation depends on stimulation by microbes and IL-10, and which mediate early-phase responses to oral antigens.

Published
2013

5. Innate lymphoid cells regulate intestinal epithelial cell glycosylation

Author

UCL - SSS/DDUV - Institut de Duve, Goto, Yoshiyuki, Kiyono, Hiroshi, Benno, Yoshimi, Umesaki, Yoshinori, Sasakawa, Chihiro, Renauld, Jean-Christophe, Becher, Burkhard, Kulig, Paulina, Domino, Steven E., Akira, Shizuo, Uematsu, Satoshi, Imaoka, Akemi, Setoyama, Hiromi, Matsuki, Takahiro, Sakamoto, Mitsuo, Kamioka, Mariko, Takeyama, Natsumi, Lamichhane, Aayam, Ivanov, Ivaylo I., Sato, Shintaro, Kunisawa, Jun, Obata, Takashi, UCL - SSS/DDUV - Institut de Duve, Goto, Yoshiyuki, Kiyono, Hiroshi, Benno, Yoshimi, Umesaki, Yoshinori, Sasakawa, Chihiro, Renauld, Jean-Christophe, Becher, Burkhard, Kulig, Paulina, Domino, Steven E., Akira, Shizuo, Uematsu, Satoshi, Imaoka, Akemi, Setoyama, Hiromi, Matsuki, Takahiro, Sakamoto, Mitsuo, Kamioka, Mariko, Takeyama, Natsumi, Lamichhane, Aayam, Ivanov, Ivaylo I., Sato, Shintaro, Kunisawa, Jun, and Obata, Takashi

Abstract

Fucosylation of intestinal epithelial cells, catalyzed by fucosyltransferase 2 (Fut2), is a major glycosylation mechanism of host-microbiota symbiosis. Commensal bacteria induce epithelial fucosylation, and epithelial fucose is used as a dietary carbohydrate by many of these bacteria. However, the molecular and cellular mechanisms that regulate the induction of epithelial fucosylation are unknown. Here, we show that type 3 innate lymphoid cells (ILC3) induced intestinal epithelial Fut2 expression and fucosylation in mice. This induction required the cytokines interleukin-22 and lymphotoxin in a commensal bacteria-dependent and -independent manner, respectively. Disruption of intestinal fucosylation led to increased susceptibility to infection by Salmonella typhimurium. Our data reveal a role for ILC3 in shaping the gut microenvironment through the regulation of epithelial glycosylation.

Published
2014

6. Innate lymphoid cells regulate intestinal epithelial cell glycosylation

Author

Goto, Yoshiyuki, primary, Obata, Takashi, additional, Kunisawa, Jun, additional, Sato, Shintaro, additional, Ivanov, Ivaylo I., additional, Lamichhane, Aayam, additional, Takeyama, Natsumi, additional, Kamioka, Mariko, additional, Sakamoto, Mitsuo, additional, Matsuki, Takahiro, additional, Setoyama, Hiromi, additional, Imaoka, Akemi, additional, Uematsu, Satoshi, additional, Akira, Shizuo, additional, Domino, Steven E., additional, Kulig, Paulina, additional, Becher, Burkhard, additional, Renauld, Jean-Christophe, additional, Sasakawa, Chihiro, additional, Umesaki, Yoshinori, additional, Benno, Yoshimi, additional, and Kiyono, Hiroshi, additional

Published
2014
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7. Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome

Author

Takemura, Naoki, primary, Kawasaki, Takumi, additional, Kunisawa, Jun, additional, Sato, Shintaro, additional, Lamichhane, Aayam, additional, Kobiyama, Kouji, additional, Aoshi, Taiki, additional, Ito, Junichi, additional, Mizuguchi, Kenji, additional, Karuppuchamy, Thangaraj, additional, Matsunaga, Kouta, additional, Miyatake, Shoichiro, additional, Mori, Nobuko, additional, Tsujimura, Tohru, additional, Satoh, Takashi, additional, Kumagai, Yutaro, additional, Kawai, Taro, additional, Standley, Daron M., additional, Ishii, Ken J., additional, Kiyono, Hiroshi, additional, Akira, Shizuo, additional, and Uematsu, Satoshi, additional

Published
2014
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10. IL-10-producing CD4+ T cells negatively regulate fucosylation of epithelial cells in the gut.

Author

Goto, Yoshiyuki, Lamichhane, Aayam, Kamioka, Mariko, Sato, Shintaro, Honda, Kenya, Kunisawa, Jun, and Kiyono, Hiroshi

Subjects
*T cells, *GLYCOSYLATION, *FUCOSYLATION, *EPITHELIAL cells, *REGENERATION (Biology)
Abstract

Fucosylated glycans on the surface of epithelial cells (ECs) regulate intestinal homeostasis by serving as attachment receptors and a nutrient source for some species of bacteria. We show here that epithelial fucosylation in the ileum is negatively regulated by IL-10-producing CD4+ T cells. The number of fucosylated ECs was increased in the ileum of mice lacking T cells, especially those expressing αβ T cell receptor (TCR), CD4, and IL-10. No such effect was observed in mice lacking B cells. Adoptive transfer of αβTCR+ CD4+ T cells from normal mice, but not IL-10-deficient mice, normalized fucosylation of ECs. These findings suggest that IL-10-producing CD4+ T cells contribute to the maintenance of the function of ECs by regulating their fucosylation. [ABSTRACT FROM AUTHOR]

Published
2015
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