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Your search keyword '"Lakshmipathy, Dhanurekha"' showing total 21 results
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21 results on '"Lakshmipathy, Dhanurekha"'

2. Role of polymerase chain reaction-based viral detection in pterygia

Author

Krishnan, Janani, Rajagopal, Rama, Lakshmipathy, Dhanurekha, Agarwal, Shweta, Anand, A., Therese, Lily, Thangam, Aishwarya, and Madhavan, Hajib

Subjects
Diagnosis, Usage, Complications and side effects, Causes of, Polymerase chain reaction -- Usage, Pterygium -- Diagnosis -- Causes of, Virus diseases -- Diagnosis -- Complications and side effects
Published
2023

12. Coexistence of Fungal Keratitis in Bilateral Sequential Microsporidial Keratitis – A Rare Case Presentation.

Author

Dhiman, Richa, Agarwal, Shweta, Anand, Appakkudal R., Lakshmipathy, Dhanurekha, Srinivasan, Bhaskar, and Iyer, Geetha

Subjects
FUNGAL keratitis, EYE infections, KERATITIS, MICROSPORIDIOSIS, MYCOSES, POLYMERASE chain reaction
Abstract

To report a case of bilateral microsporidiosis with coexisting fungal infection in one eye. Retrospective interventional case report. A 61-year-old man with uncontrolled diabetes presented with clinical and microbiological features of non-resolving fungal keratitis in the right eye since 3 months and underwent therapeutic penetrating keratoplasty (TPK) for the same. Fungal filaments along with oval bodies suspicious of microconidia were noted on calcofluor stain. A week following TPK, the patient presented with features of viral keratouveitis in the left eye which on microbiology was confirmed as microsporidiosis. Retrospectively, the right eye microbiology slides were reassessed, which confirmed the coexistence of fungus with microsporidiosis by acid-fast stain and polymerase chain reaction. Structural resemblance of microconidia with microsporidial spores can be misleading, thus creating a need for awareness regarding the possible coexistence along with a need to suspect microsporidiosis in nonresponding clinically resembling viral keratitis. [ABSTRACT FROM AUTHOR]

Published
2022
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13. First report of causing fungal keratitis from a tertiary eye hospital in India.

Author

Lily Therese, Kulandai, Lakshmipathy, Meena, and Lakshmipathy, Dhanurekha

Subjects
ANTIFUNGAL agents, HOSPITALS, FUNGI, MYCOSES, EYE infections
Abstract

A young 33 year old male presented with non-resolving corneal infiltrate for 2 month duration in the right eye. KOH/ Calcoflour wet mount revealed sparsely septate fungal hyphae. Post therapeutic penetrating keratoplasty 3 doses of intracameral voriconazole(100μg/0.1ml) was administered suspecting recurrence. Fungal culture revealed non sporulating mould on SDA. PCR based DNA sequencing targeting the ITS region identified the fungal isolate as Mortierella wolfii (M. wolfii) belonging to zygomycetes. To the best of our knowledge, this is the first report of human fungal keratitis caused by M. wolfii. [ABSTRACT FROM AUTHOR]

Published
2020
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20. Nested reverse transcriptase–polymerase chain reactions targeting the messenger RNA of icl2, hspx, and rRNAP1genes to detect viable Mycobacterium tuberculosisdirectly from clinical specimens

Author

Lakshmipathy, Dhanurekha, Kulandai, Lily Therese, Ramasubban, Gayathri, Hajib Narahari Rao, Madhavan, Rathinam, Sridhar, and Narasimhan, Meenakshi

Abstract

There is an urgent need for a rapid and reliable test to detect actively multiplying Mycobacterium tuberculosisdirectly from clinical specimens for an early initiation of the appropriate antituberculous treatment. This study was aimed at the optimization and application of nested reverse transcriptase–PCR (nRT–PCR) targeting the messenger RNA of the icl2, hspx, and rRNAP1genes directly from sputum specimens, and their evaluation against the culture by the BACTEC MicroMGIT mycobacterial culture system. 203 Sputum samples from clinically suspected tuberculosis patients and 30 control specimens (clinically proven viral or bacterial infections other than tuberculosis) were included in this study. The mycobacterial culture was performed by the BACTEC MicroMGIT system following the manufacturer’s instructions. The primers for nRT–PCRs targeting icl2, hspx, and rRNAP1genes were indigenously designed using the Primer-BLAST software, and optimized for sensitivity and specificity. The icl2, hspx, and rRNAP1genes were able to pick up 63.9%, 67.2%, and 58.75%, respectively, of culture-negative sputum specimens collected from clinically suspected tuberculosis patients. However, three (1.4%) were negative for nRT–PCR, but M. tuberculosis culture positive. All the 30 controls were negative for culture by the BACTEC MicroMGIT method and all three nRT–PCR. The novel nRT–PCRs targeting icl2, hspx, and rRNAP1genes developed in this study are rapid and reliable diagnostic tools to detect viable M. tuberculosisdirectly from sputum specimens. However, further study by including a larger number of sputum specimens needs to be carried out to ascertain the diagnostic utility of the novel nRT–PCRs optimized in the study.

Published
2015
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21. Detection of novel and reported mutations in the rpoB, katGand inhAgenes in multidrug-resistant tuberculosis isolates: A hospital-based study

Author

Ramasubban, Gayathri, Therese, Kulandai Lily, Lakshmipathy, Dhanurekha, Sridhar, R., Meenakshi, N., and Madhavan, Hajib N.

Abstract

•This was a Pilot study conducted to find out the mutations targeting rpoB, katGand inhAgene by PCR based DNA technique in M. tuberculosisisolates.•Only 37.5% of RIF-resistant isolates showed the presence of Ser531Leu mutation.•The frequency of mutations associated with INH resistance was 31.5% and 29.6% targeting katGand I gene.

Published
2015
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