1. High-throughput screening of encapsulated islets using wide-field lens-free on-chip imaging
- Author
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Zhang, Yibo, Alexander, Michael, Yang, Sam, Bian, Yinxu, Botvinick, Elliot, Lakey, Jonathan R. T., and Ozcan, Aydogan
- Subjects
Physics - Instrumentation and Detectors ,Electrical Engineering and Systems Science - Image and Video Processing ,Physics - Applied Physics ,78A10 - Abstract
Islet microencapsulation is a promising solution to diabetes treatment, but its quality control based on manual microscopic inspection is extremely low-throughput, highly variable and laborious. This study presents a high-throughput islet-encapsulation quality screening system based on lens-free on-chip imaging with a wide field-of-view of 18.15 cm^2, which is more than 100 times larger than that of a lens-based optical microscope, enabling it to image and analyze ~8,000 microcapsules in a single frame. Custom-written image reconstruction and processing software provides the user with clinically important information, such as microcapsule count, size, intactness, and information on whether each capsule contains an islet. This high-throughput and cost-effective platform can be useful for researchers to develop better encapsulation protocols as well as perform quality control prior to transplantation.
- Published
- 2018
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