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120 results on '"Lahoz, E."'

4. Preliminar Assessment of Durability for Aluminium Composite Panels

Author

Lahoz, E., Hernández de Olivares, F., Llinares-Millán, Carmen, editor, Fernández-Plazaola, Igor, editor, Hidalgo-Delgado, Francisco, editor, Martínez-Valenzuela, María Montserrat, editor, Medina-Ramón, Francisco Javier, editor, Oliver-Faubel, Inmaculada, editor, Rodríguez-Abad, Isabel, editor, Salandin, Andrea, editor, Sánchez-Grandia, Rafael, editor, and Tort-Ausina, Isabel, editor

Published
2014
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41. Use of puromycin N-acetyltransferase (PAC) as a new reporter gene in transgenic animals

Author

Gómez Lahoz, E, López de Haro, M S, Nieto, A, Esponda, P, and Dirección General de Investigación Científica y Técnica, DGICT (España)

Subjects
Reporter gene, Transgene, Acetyl-CoA, Mice, Transgenic, Transfection, Biology, biology.organism_classification, Molecular biology, Chloramphenicol acetyltransferase, chemistry.chemical_compound, Streptomyces alboniger, Mice, chemistry, Puromycin, Acetyltransferases, Genetics, Animals, Homogenization (biology)
Abstract

1 p.-1 tab., This work was partially supported by grants No. PB87-0236-C02-01 and PB87-0236-C02-02 from the DGICYT, Spain.

Published
1991

47. Tissue-specific and hormonally regulated expression of the puromycin N-acetyltransferase-encoding gene under control of the rabbit uteroglobin promoter in transgenic mice

Author

Dirección General de Investigación Científica y Técnica, DGICT (España), Gómez Lahoz, E., López de Haro, M. S., Esponda, Pedro, Nieto, A., Dirección General de Investigación Científica y Técnica, DGICT (España), Gómez Lahoz, E., López de Haro, M. S., Esponda, Pedro, and Nieto, A.

Abstract

Transgenic mice bearing two fragments of the rabbit uteroglobin 5'-flanking region fused to the new reporter gene (pac) encoding puromycin N-acetyltransferase (PAC) showed a different pattern of expression. Transgenic lines (C0.4) harboring a 404-bp fragment (-396/+8) had a uterus-specific expression slightly inducible by estrogen, lacking detectable expression in other tissues where the uteroglobin-encoding gene is naturally expressed in rabbit. Transgenic lines (C3.2) bearing a longer fragment of 3.2-kb (-3254/+8) showed hormonally regulated expression in the uterus and the male genital tract, and detectable expression in the lung. In addition, the nonstimulated uterine expression of the transgene was higher in C0.4 lines than in C3.2 lines. It could be concluded that all sequences required for uterus-specific expression should be present within the 404-bp fragment, and that other upstream (-396) sequences are responsible for expression in the lung and male genital tract, as well as for a possible down modulation of expression in the uterus.

Published
1992

48. Immunocytochemical localization of phospholipase A2 in hamster spermatozoa

Author

Dirección General de Investigación Científica y Técnica, DGICT (España), Consejo Superior de Investigaciones Científicas (España), Universidad de Chile, Riffo, M., Gómez Lahoz, E., Esponda, Pedro, Dirección General de Investigación Científica y Técnica, DGICT (España), Consejo Superior de Investigaciones Científicas (España), Universidad de Chile, Riffo, M., Gómez Lahoz, E., and Esponda, Pedro

Abstract

Antibodies raised against porcine pancreatic phospholipase A2 (PLA2) react in immunoblottings with both the antigen as well as with one protein band of about 14 kDa from hamster spermatozoa extracts. Immunoblottings of proteins extracted from spermatozoon head and tail fractions also show similar results. Anti-PLA2 purified IgGs were employed for light and electron microscopic immunocytochemistry in order to detect PLA2 in hamster cauda epididymal spermatozoa. When whole mount spread spermatozoa were used under light (employing the PAP complex) or electron microscopy (using anti-rabbit gold conjugated), the acrosomal area of the gametes shows a noticeable labelling; a characteristic which is not observed in samples treated with the pre-immune serum. Immunocytochemistry undertaken in ultrathin sections from spermatozoon samples embedded in Lowicryl, demonstrates that the antigen appears preferentially distributed in the acrosome. Besides, sperm tails showed a scattered distribution of gold granules in the mitochondria of the midpiece. Results suggest that the antibody used recognizes a PLA2 which is preferentially located in the acrosome and mitochondria. On the other hand, the presence of a surface PLA2 in the plasma membrane covering the acrosome is suggested. This surface PLA2 would be probably related to the acrosome reaction phenomenon that occurs in the spermatozoon before penetrating the oocyte.

Published
1992

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